Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.58260561
Arnt 2 interacted with AhR and mouse Sim as efficiently as Arnt , and the Arnt 2 AhR complex recognized and bound specifically the xenobiotic responsive element ( XRE ) sequence . 0.58260561^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :1.0089184
In addition , comparisons between AhR and ARNT transcripts isolated from the liver and splenocytes indicated a greater number of ARNT transcripts as compared with AhR in both preparations . 1.0089184^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.59588674
The aryl hydrocarbon ( or dioxin ) receptor ( AhR ) is a ligand activated basic helix loop helix ( bHLH ) protein that heterodimerizes with the bHLH protein AhR nuclear translocator ( ARNT ) to form a complex that binds to xenobiotic regulatory elements in the enhancers of target genes . 0.59588674^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.76362442
The purpose of this study was to determine whether covalent modifications of ARNT occur between ARNT existing in the monomeric form and after heterodimerization with the AhR and subsequent binding to DNA . 0.76362442^^^ The results indicated that ARNT complexed with the AhR in the nucleus has an isoform pattern shifted toward the basic end , with the predominant isoform having a pI of 6 . 8 . 0.53160189^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.57792588
This indicates that complex formation of AhR with Arnt on DNA is an important and critical step in transforming AhR into a high affinity receptor for TCDD . 0.57792588^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :1.111758
In an earlier study , murine ARNT was shown to be a phosphoprotein , to display charge heterogeneity , and to have a shift in its predominant isoforms after heterodimerization with the AhR . 1.111758^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.55818542
In the present study , we showed that in U 937 cells and human macrophages AhR , with its partner cofactor Arnt , is expressed and CYP1A1 mRNA expression is induced in the presence of AhR ligand 3MC . 0.55818542^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.6094961
The interaction of AhR with ARNT was both necessary and sufficient for the recruitment of AhR to transcription sites . 0.6094961^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.58712415
In this study , we wanted to determine if another bHLH PAS transcription factor , ARNT 2 , which has high amino acid sequence identity to ARNT and has been shown to dimerize with the TCDD activated AHR , is involved in mediating TCDD ' s effect on lymphocyte development . 0.58712415^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.58585848
ARNT bound AHR or PML , whereas the sumoylated form of ARNT associated with AHR , but not with PML , resulting in a reduced effect of PML on transactivation by ARNT . 0.58585848^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.57999366
We produced Saccharomyces cerevisiae coexpressing mouse AhR and aryl hydrocarbon receptor nuclear translocator ( Arnt ) protein in accordance with Miller 3 ' s method for constructing yeasts with human Ahr and Arnt [ Toxicol . 0.57999366^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.51834552
When in vitro synthesized ARNT was added concomitantly with TCDD to C cytosolic extracts , it associated with the Ah receptor and restored Ah receptor dependent XRE binding activity to the extracts . 0.51834552^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.60229376
In the presence of 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin ( TCDD ) and related chemicals , the Ah receptor nuclear translocator ( Arnt ) forms a heterodimeric complex with the ligand bound Ah receptor , leading to recognition of dioxin responsive elements within the enhancer of the CYP1A1 gene and transcription activation by an unknown mechanism . 0.60229376^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.64938695
ARNT dimerizes with several PAS superfamily members , including the ligand activated aryl hydrocarbon receptor ( AHR ) , forming a complex that alters transcription by binding specific elements within the promoters of target genes . 0.64938695^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.55105928
The aryl hydrocarbon nuclear translocator ( Arnt ) is a basic helix loop helix ( per / Arnt / Ahr / sim ) PAS containing protein that can heterodimerize with the aryl hydrocarbon receptor ( AhR ) , the hypoxia inducible factor 1 alpha , and other PAS containing proteins to form transcriptionally active complexes . 0.55105928^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.55626778
The aryl hydrocarbon receptor ( AHR ) interacts with the aryl hydrocarbon receptor nuclear translocator ( ARNT ) to form a heterodimer that binds to promoters in target genes to alter transcription in response to xenobiotics such as 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin ( TCDD ) . 0.55626778^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor ( AHR ) mediates dioxin ( 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin ) induced transcriptional activation of a battery of genes by interaction with a cofactor , called aryl hydrocarbon receptor nuclear translocator ( ARNT ) protein . ^^^ Both AHR and ARNT belong to a family of proteins that includes the Drosophila circadian rhythm protein and `` single minded ' ' protein . ^^^ In addition to the PAS domain , both AHR and ARNT contain basic helix loop helix ( bHLH ) and glutamine ( Q ) rich domains . ^^^ By using the yeast two hybrid system with the N terminal half of AHR as a probe , which contains the bHLH and PAS regions , to screen cDNA libraries prepared from human lymphocytes and C57BL mouse liver for clones encoding proteins capable of binding to these regions , we isolated a partial ARNT cDNA clone . ^^^ These results demonstrated that the N terminal half of AHR is capable of interacting with ARNT in yeast ( probably through the bHLH motif ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The ligand mediated dissociation of Ahr from the 90 kDa heat shock protein ( HSP 90 ) does not require the aryl hydrocarbon receptor nuclear translocator ( Arnt ) , but it is slightly enhanced by this protein . ^^^ In order to minimize any artificial steric hindrances to dimerization and XRE binding , each Ahr mutant was also tested with an equivalently deleted Arnt mutant . ^^^ Biol . 14 , 6075 6086 ) compatible with the notion that equivalent regions of Ahr and Arnt associate with each other . ^^^ Deletion of the carboxyl terminal half of Ahr does not affect dimerization or XRE binding but , in contrast to an equivalent deletion of Arnt , eliminates biological activity as assessed by an in vivo transcriptional activation assay , suggesting that this region of Ahr plays a more prominent role in transcriptional activation of the cyp1a1 gene than the corresponding region of Arnt . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The genomic clones of human aryl hydrocarbon receptor ( Ahr ) and Ahr nuclear translocator ( Arnt ) were isolated , and the structures of exon intron junctions of these genes were partially determined . ^^^ Based on the sequence information , a quantitative RT PCR analysis was developed , and the expression of these genes was studied in various human tissues . mRNAs for Ahr and Arnt were widely expressed in human tissues and abundantly in lung . ^^^ Individual difference in expression levels of Ahr and Arnt mRNA was observed in liver , lung and blood . ^^^ In order to examine whether expression levels of Ahr and Arnt were associated with those of CYP1A1 , we studied the expression of these mRNAs in blood among 20 healthy subjects , taking account of individuals ' cigarette smoking habits . ^^^ We found that the expression levels of CYP1A1 appeared to associate with those of Ahr and Arnt mRNAs ( P < 0 . 06 ) , and also that the expression of Ahr and Arnt was influenced by cigarette smoking . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
We show that both HIF 1 subunits are basic helix loop helix proteins containing a PAS domain , defined by its presence in the Drosophila Per and Sim proteins and in the mammalian ARNT and AHR proteins . ^^^ HIF 1 beta is a series of ARNT gene products , which can thus heterodimerize with either HIF 1 alpha or AHR . ^^^ HIF 1 beta is a series of ARNT gene products , which can thus heterodimerize with either HIF 1 alpha or AHR . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Individual difference in expression levels of Ahr and Arnt mRNAs was observed , and the expression levels of CYP1A1 appeared to associate with those of transcriptional factors . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The Ah receptor ( AHR ) , the Ah receptor nuclear translocator protein ( ARNT ) , and single minded protein ( SIM ) are members of the basic helix loop helix PAS ( bHLH PAS ) family of regulatory proteins . ^^^ Comparison of this in vitro data with sites known to be bound in vivo suggests that the high affinity half site recognition sequences for the AHR , SIM , and ARNT are T ( C / T ) GC , GT ( G / A ) C ( 5 ' half sites ) , and GTG ( 3 ' half sites ) , respectively . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Binding of polycyclic or halogenated aromatic hydrocarbon ( PAH and HAH ) ligand causes release of AHR , which then associates with the AHR nuclear translocator protein ( ARNT ) to generate the heterodimeric `` transformed ' ' AHRC . ^^^ AHR and ARNT belong to a novel subclass of basic helix loop helix containing transcription factors . ^^^ Current research includes investigations directed towards delineating the pathways of HAH pathogenesis , ascertaining whether AHR can mediate signal transduction independently of DNA binding , understanding the mechanism of transcriptional activation , and investigating the potential roles of AHR and ARNT in development . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aromatic hydrocarbon receptor nuclear translocator ( ARNT ) is a basic helix loop helix PAS protein which forms a heterodimer with aromatic hydrocarbon receptor ( AHR ) , this heterodimer mediating the signal transduction in response to the various xenobiotics such as 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin and directly interacting with target genes by binding to xenobiotic responsive elements . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Both components of the transformed AHR complex [ the ligand binding AHR monomer and the AHR nuclear translocator ( ARNT ) ] directly contact the XRE . ^^^ The binding sites of AHR and ARNT on the asymmetric XRE were determined using nuclear extracts of 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin treated Hepa 1c1c7 cells and a panel of double stranded oligonucleotides containing XRE 1 of the CYP1A1 gene ( 5 ' TTGCGTGAGAA 3 ' ) , in which all combinations of three , two , or one of the thymines indicated were substituted by the photoreactive thymine analog 5 bromodeoxyuracil . ^^^ Covalent cross linking analysis and immunoprecipitation with antibodies specific for AHR or ARNT demonstrated that ARNT directly contacts the 3 ' most thymine position , that AHR directly contacts the second thymine position , and that neither protein contacts the 5 ' most thymine position . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor ( AhR ) and the AhR nuclear translocator protein ( ARNT ) are basic helix loop helix PAS ( HLH ) proteins involved in transcriptional regulation . ^^^ This complex dissociates following ligand binding and then the ligand bound AhR binds ARNT . ^^^ The ligand AhR ARNT complex interacts with a specific , nuclear DNA sequence , the dioxin response element ( DRE ) , altering transcription of a regulated gene . ^^^ In this study , AhR and ARNT were localized immunohistochemically in human embryonic palatal cells and specific patterns of expression were seen for each protein . ^^^ A double staining protocol revealed that epithelial cells expressed both AhR and ARNT , but in mesenchyme and nasal spine cartilage individual cells were identified which expressed either AhR or ARNT . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Analyses of deletion mutants indicate that the carboxyl half of AhR contains several types of transactivation domain , which function independently of domains that mediate TCDD recognition , DNA binding , and heterodimerization with the Ah receptor nuclear translocator ( Arnt ) protein . ^^^ The inhibitory domain displays specificity , in that it blocks the transactivating functions of AhR and Arnt , but not that of the herpes simplex protein VP 16 . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Then the expression levels of AhR mRNA in various hematopoietic cell lines were examined together with those of Arnt and P450IA1 . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In mouse hepatoma cells , the environmental contaminant 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin ( TCDD , or dioxin ) induces Cyp1A1 gene transcription , a process that requires two basic helix loop helix regulatory proteins , the aromatic hydrocarbon receptor ( AhR ) and the aromatic hydrocarbon receptor nuclear translocator ( Arnt ) . ^^^ Dioxin induced binding of the AhR / Arnt heteromer to enhancer chromatin is associated with a localized ( about 200 bp ) alteration in chromatin structure that is manifested by increased accessibility of the DNA ; these changes probably reflect direct disruption of a nucleosome by AhR / Arnt . ^^^ Dioxin induces analogous AhR / Arnt dependent changes in chromatin structure and accessibility at the Cyp1A1 promoter . ^^^ However , the changes at the promoter must occur by a different , more indirect mechanism , because they are induced from a distance and do not reflect a local effect of AhR / Arnt binding . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Two years have passed since the Ah receptor ( AhR ) and the AhR nuclear translocator ( Arnt ) were revealed to constitute a novel ligand activated transcription factor by molecular biological studies on cloned cDNAs . ^^^ Many interesting properties of the AhR / Arnt system have begun to be elucidated . ^^^ We present here a brief summary of the molecular biology of AhR and Arnt . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Arnt ( Ah receptor nuclear translocator ) is a member of a transcription factor family having characteristic motifs designated bHLH ( basic helix loop helix ) and PAS and was originally found as a factor forming a complex with Ah receptor ( AhR ) to bind the specific xenobiotic responsive element ( XRE ) sequence for induction of drug metabolizing P4501A1 . ^^^ We have examined interaction of Arnt with other PAS proteins Drosophila Per , Sim , and AhR by the coimmunoprecipitation method . ^^^ The Arnt homodimer binds the sequence of adenovirus major late promoter ( MLP ) with the E box core sequence CACGTG , suggesting that the CAC half of the XRE , CACGCN ( A / T ) , recognized by the AhR Arnt heterodimer is a target for Arnt . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In the presence of ligand , AhR interacts with its dimerization partner , Arnt , and the heteromeric complex is able to bind DNA . ^^^ In the present study , we test the effects of single base pair substitutions of site D on the ability of the AhR / Arnt heteromer to recognize this response element using an in vitro transcription system . ^^^ Synthetic oligodeoxyribonucleotides corresponding to the wild type sequence of site D , or single base pair mutations of that sequence , were used to compete for AhR / Arnt binding with the transcription template . ^^^ Using this competition assay , the sequence of the core recognition motif 5 ' GCGTG 3 ' was shown to be critical for AhR / Arnt binding , and the importance of the position and orientation of the G : C and A : T base pairs of this sequence was determined . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In an effort to facilitate the structural and biochemical analyses of the Ah receptor ( AHR ) and the Ah receptor nuclear translocator ( ARNT ) , a baculovirus system was developed to express microgram milligram quantities of the human version of these proteins . ^^^ Coexpression of the AHR and ARNT in Sf 9 cells resulted in the in vivo generation of heterodimers that bound the DRE in the absence of ligand . ^^^ Studies with the nickel nitriloacetic acid purified recombinant proteins demonstrated that the AHR and ARNT could bind DRE only when reconstituted with a heat sensitive factor ( s ) present in soluble extracts from a variety of cell types . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The ligand binding component is the AhR protein , a cytosolic receptor encoded by the Ahr gene , which , upon ligand binding , translocates to the nucleus in a heterodimeric complex with the ARNT ( Ah receptor nuclear translocator ) component . ^^^ This protein was not AhR nor was it ARNT , since it was found in receptorless ( Ahr ) and in nuclear translocation defective ( Arnt ) cells , as well as in cells that had not been exposed to 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin ( TCDD ; dioxin ) , a potent inducer of Cyp1a1 expression . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In an effort to provide a more powerful system to study the Ah receptor ( AHR ) signaling pathway , we expressed the AHR , its dimerization partner ARNT , and a beta galactosidase ( lacZ ) reporter gene , driven by two dioxin responsive enhancers , in the yeast Saccharomyces cerevisiae . ^^^ To accomplish this in a manner that was independent of the ARNT protein , we constructed a chimeric receptor in which the DNA binding and primary dimerization domains of the AHR were swapped with analogous domains from the LexA protein . ^^^ Coexpression of this AHR LexA chimera and a lacZ reporter gene driven by eight LexA operator sites in a yeast strain with regulatable levels of hsp 90 , yielded pharmacology that closely mirrored that of the AHR / ARNT / dioxin responsive enhancer system described above , but only when hsp 90 levels were held near their wild type levels . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The Ah receptor ( AHR ) is a ligand activated transcription factor that is structurally related to its dimerization partner , the Ah receptor nuclear translocator ( ARNT ) , and two Drosophila proteins , SIM and PER . ^^^ In addition , the AHR , ARNT , and SIM harbor a basic region helix loop helix motif in their N termini , whereas PER does not . ^^^ To identify domains involved in transactivation of target genes , we generated chimeras of AHR / ARNT deletion mutants with the DNA binding region of the yeast Gal 4 protein , transiently expressed these in COS 1 cells , and monitored their capacity to activate the chloramphenicol acetyltransferase reporter gene under the control of a minimal promoter driven by enhancer elements recognized by Gal 4 . ^^^ Extensive analysis of these fusions revealed that the AHR and ARNT harbor potent transactivation domains within their C termini . ^^^ Importantly , the amino terminal halves of both the AHR and ARNT were found to be devoid of transactivation activity . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The activated aryl hydrocarbon receptor ( AHR ) and the AHR nuclear translocator ( ARNT ) bind DNA as a heterodimer . ^^^ Both proteins represent a novel class of basic helix loop helix ( bHLH ) containing transcription factors in that ( 1 ) activation of AHR requires the binding of ligand ( e . g . , 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin [ TCDD ] ) , ( 2 ) the xenobiotic responsive element ( XRE ) recognized by the AHR / ARNT heterodimer differs from the recognition sequence for nearly all other bHLH proteins , and ( 3 ) both proteins contain a PAS homology region , which in the Drosophila PER and SIM proteins functions as a dimerization domain . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Upon binding agonists , the AHR dimerizes with a structurally related protein known as ARNT and this heterodimer then binds cognate enhancer elements and activates the expression of target genes . ^^^ In this report we describe the cloning of the rat AHR cDNA and a fragment of the rat ARNT cDNA for use as probes in ribonuclease protection analysis . ^^^ The rat AHR and ARNT mRNAs were expressed in a largely coordinate manner across the eight tissues examined with the exception of the placenta where AHR levels were relatively low compared to ARNT . ^^^ In vitro expression studies demonstrated that in contrast to the full length AHR , the splice variant did not bind ligand nor did it bind to a cognate enhancer element in the presence of ARNT . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor ( AhR ) and AhR nuclear translocator ( Arnt ) protein were evaluated in the Hepa 1c1c7 ( Hepa 1 ) cell line by indirect immunofluorescence microscopy and Western blot analysis . ^^^ Detailed evaluation by immunoelectron microscopy of the AhR and Arnt present in the nuclear compartment of WT cells shows that both proteins are uniformly distributed and do not appear to be associated with nuclear pores , membranes , or nucleoli . ^^^ Western blot analysis of nuclei isolated from WT Hepa 1 cells fractionated with Nonidet P 40 shows that minimal levels of AhR or Arnt are retained in the nuclear fraction after TCDD treatment . ^^^ Collectively , these results indicate that the unliganded AhR resides in the cytoplasm , Arnt is localized to the nucleus , and Hepa 1 cells defective in Arnt expression exhibit TCDD mediated nuclear accumulation of the AhR . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
However , suppression was reestablished following transfection of the human Ah receptor nuclear translocator ( ARNT ) complementary DNA expression vector into C 4 cells and the mouse Ah receptor ( AhR ) complementary DNA expression vector into C 12 cells . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The original observations about genetic differences in CYP1A1 ( cytochrome P 1 450 ) induction by TCDD or benzo [ a ] pyrene in the mouse have led to an appreciation for a similar polymorphism in the human and the recent cloning of the murine Ah receptor ( Ahr ) and human Ah receptor nuclear translocator ( ARNT ) genes . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In this report , we describe a rabbit reticulocyte system that allows functional expression of both the AHR and its dimeric partner , the AHR nuclear translocator protein ( ARNT ) . ^^^ By using this in vitro system , we were able to reconstitute agonist binding to the AHR and agonist induced AHR ARNT recognition of a cognate DNA enhancer sequence . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Both functions require heterodimerization between AhR and the AhR nuclear translocator ( Arnt ) . ^^^ Our findings also indicate that heterodimerization activates AhR ' s latent transactivation function and silences that of Arnt . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Experiments using a GST fusion protein demonstrated that mSim heterodimerizes with Arnt ( Ah receptor nuclear translocator ) , even more efficiently than AhR ( Ah receptor ) does with Arnt . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Restoration of Ah responsiveness was investigated by co transfecting Hs578T cells with pRNH11c or pMCAT5 . 12 and plasmids that express the ER ( hER ) , Ah receptor ( AhR ) and AhR nuclear translocator ( Arnt ) proteins . ^^^ Expression of the AhR or Arnt proteins did not alter basal or inducible CAT activity . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor ( AhR ) and the AhR nuclear translocator protein ( ARNT ) are basic helix loop helix ( bHLH ) proteins involved in transcriptional regulation . ^^^ The AhR is a ligand activated partner of the ARNT protein . ^^^ ARNT must be complexed to AhR to permit binding to the regulatory DNA sequence . ^^^ AhR and ARNT are expressed in human embryonic palatal cells and AhR was recently shown to have a specific developmental pattern of expression in the mouse embryo . ^^^ These specific patterns of ARNT expression , which differ in certain tissues from the expression of AhR , suggest that ARNT may have additional roles in normal embryonic development . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor ( AHR ) and the aryl hydrocarbon receptor nuclear translocator ( ARNT ) belong to a novel subclass of basic helix loop helix transcription factors . ^^^ Amino acid positions in these AHR regions and in the ARNT basic region less adversely affected by substitution are also identified . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Induction requires two basic helix loop helix proteins , the ligand binding aromatic hydrocarbon receptor ( AhR ) and its heterodimerization partner , the AhR nuclear translocator ( Arnt ) . ^^^ The AhR / Arnt heterodimer induces transcription by binding to dioxin responsive elements ( DREs ) within an enhancer upstream of the CYP1A1 gene . ^^^ The basic regions of AhR and Arnt are crucial for DRE binding . ^^^ We have mutated these regions in order to analyze the relationship between DRE binding ( determined in vitro using an electrophoretic mobility shift assay ) and induction of CYP1A1 transcription ( determined in vivo by genetic complementation of AhR defective and Arnt defective mouse hepatoma cells , using an RNase protection assay to measure mRNA accumulation ) . ^^^ Our findings reveal the amino acids in the basic regions of AhR / Arnt that are important for both DRE binding and induction of transcription . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Inducible activation of the CYP1A1 promotor is mediated by a ligand dependent transcription factor dimer complex including the aryl hydrocarbon receptor ( AHR ) and the AHR nuclear translocator ( ARNT ) proteins . ^^^ RT PCR analyses did not indicate any changes in mRNA level of AHR and ARNT in the co transfection studies . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor ( AHR ) is a ligand activated transcription factor that binds DNA in the form of a heterodimer with the AHR nuclear translocator protein ( ARNT ) . ^^^ ARNT binds to the side of the xenobiotic responsive element ( XRE ) that resembles an E box ( the sequence recognized by the majority of other basic helix loop helix proteins ) , whereas AHR binds to the side of the XRE that does not conform to the E box sequence . ^^^ The basic region of ARNT closely resembles those of other E box binding proteins , whereas the `` nominal basic region ' ' of AHR ( amino acids 27 39 ) , although required for XRE binding , deviates from this consensus . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Western blot analysis and indirect immunofluorescence microscopy were used to evaluate the fate of the aryl hydrocarbon receptor ( AhR ) and aryl hydrocarbon receptor nuclear translocator ( Arnt ) protein in culture cell models exposed to 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin ( TCDD ) . ^^^ These results indicate that the liganded AhR is rapidly depleted within the nuclear compartment of hepatic and nonhepatic cells in a manner independent of the Arnt protein . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The trans acting factor on the XRE is a heterodimer consisting of arylhydrocarbon receptor ( AhR ) and AhR nuclear translocator ( Arnt ) , while Sp 1 acts as a regulatory factor on the BTE . ^^^ Both in vivo transfection assays using Drosophila Schneider line 2 ( SL 2 ) cells , which is devoid of endogenous Sp 1 , AhR , and Arnt , and in vitro transcription assays using baculovirus expressed AhR , Arnt , and Sp 1 proteins revealed that these factors enhanced synergistically expression of the reporter genes driven by a model CYP1A1 promoter , consisting of four repeated XRE sequences and a BTE sequence , in agreement with previous observation ( Yanagida , A . , Sogawa , K . , Yasumoto , K . , and Fujii Kuriyama , Y . ( 1990 ) Mol . ^^^ We have proved by coimmunoprecipitation assays and DNase 1 footprinting that both AhR and Arnt interact with the zinc finger domain of Sp 1 via their basic HLH / PAS domains . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The Ah receptor ( AhR ) is a ligand dependent transcription factor subunit that heterodimerizes with the AhR nuclear translocator ( Arnt ) and mediates the predominant biological effects of 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin ( TCDD ) . ^^^ Single and double hybrid analysis dissociates AhR ligand responsiveness , transactivation , and heterodimerization with Arnt from receptor binding to an XRE . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Dioxins exert their effects through a ligand activated transcription factor termed the dioxin or aryl hydrocarbon receptor ( Ahr ) , which acts in concert with another structurally related protein : the aryl hydrocarbon nuclear translocator ( Arnt ) . ^^^ We found mRNAs for both Ahr and Arnt predominantly in the same neuronal populations : in the olfactory bulb , the hippocampus , and the cerebral and cerebellar cortices . ^^^ Arnt , however , had a more widespread expression than Ahr in the brain . ^^^ Furthermore , it appears that Arnt may have additional functions in the brain , apart from being the heterodimerization partner of Ahr , possibly through heterodimerizing with other transcription factors . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
A competent AhR comprises at least two components following nuclear translocation and DNA binding , the AhR and the AhR nuclear translocator ( Arnt ) protein , whose combined action on human CYP1A1 gene transcription is shown to be dependent upon functional protein kinase C ( PKC ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
ADSS also induced EROD in wild type mouse hepatoma ( Hepa1c1c7 ) cells ( hepa 1 ) , but not in variant hepa 1 cells defective in the AhR nuclear translocator ( ARNT ) protein . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In mouse hepatoma Hepa 1 cells , which have Ah receptor ( AHR ) and Ah receptor nuclear translocator ( ARNT ) proteins , inclusion of TRE , SRE , and the AhRE motifs from c jun and junD , but not CRE or the AhREs from c fos , fosB , and junB , causes a large TCDD dependent increase in luciferase expression . ^^^ In agreement with these results , c jun and junD , but not c fos , fosB , and junB AhREs , competed with a canonical Cyp1A1 AhRE for binding to the AHR ARNT heterodimeric complex . ^^^ In African Green Monkey CV 1 cells , which lack AHR , expression plasmids with AhRE motifs require coexpression of AHR and ARNT for TCDD to stimulate luciferase expression . ^^^ In contrast , SRE containing expression plasmids respond equally well to TCDD whether or not AHR and ARNT are coexpressed . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Yeast two hybrid analysis and coimmunoprecipitation experiments demonstrated that both of the mSim gene products interacted with Arnt even more efficiently than AhR , a natural partner of Arnt , suggesting a functional cooperativity with Arnt . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The Ah receptor ( AHR ) and its DNA binding partner , the Ah receptor nuclear translocator ( ARNT ) , are basic helix loop helix proteins distinguished by their PER , AHR , ARNT , and SIM ( PAS ) homology regions . ^^^ ARNT heterodimer that contact the TNGCGTG recognition sequence , we have performed deletion mapping and amino acid substitutions within the N termini of both the AHR and ARNT . ^^^ The ability of the variant AHR and ARNT proteins to bind DNA and activate gene transcription was determined by the gel shift analysis and transient transfection assays . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The transcript levels of the AhR and its associated translocator protein ( ARNT ) were found to increase with ongoing differentiation in the human keratinocyte cell line HaCaT . ^^^ When keratinocytes were continuously treated with 1 microM retinoic acid ( RA ) , the upregulation of AhR and ARNT mRNA levels was inhibited as was keratin 4 expression , a marker of HaCaT keratinocyte differentiation . ^^^ Our data suggest that the regulation of AhR , ARNT and keratin 4 expression by RA is indirect and mediated by a yet to be identified factor . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
We previously reported that the aryl hydrocarbon receptor ( AhR ) complex , composed of the AhR ligand binding subunit and the Ah receptor nuclear translocator ( ARNT ) , was constitutively present in nuclear extracts from two human B lymphocyte cell lines ( Biochem . ^^^ In contrast , the nuclear DNA binding AhR complex in IM 9 and PJS 91 cells consisted of several closely migrating species , one being recognized by an AhR antibody , while an ARNT antibody reacted with all species . ^^^ Protein : DNA cross linking analysis revealed the presence of a novel Mr 100 , 000 DNA binding protein in nuclear extracts from IM 9 and PJS 91 , but not HepG 2 , cells that was not recognized by either AhR or ARNT antibodies . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Drosophila single minded , which acts as a positive master gene regulator in central nervous system midline formation in Drosophila , its two mouse homologs SIM 1 and SIM 2 , and the mammalian aryl hydrocarbon receptor ( AHR ) and aryl hydrocarbon receptor nuclear translocator ( ARNT ) proteins are members of the basic helix loop helix . ^^^ In the yeast two hybrid system , we demonstrate strong constitutive interaction of ARNT with SIM 1 and SIM 2 and fully ligand dependent interaction of ARNT with AHR . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Expression of aryl hydrocarbon receptor ( AhR ) and aryl hydrocarbon receptor nuclear translocator ( Arnt ) in adult rabbits known to be non responsive to cytochrome P 450 1A1 ( CYP1A1 ) inducers . ^^^ In the present study , we isolated cDNA clones encoding aryl hydrocarbon receptor ( AhR ) and aryl hydrocarbon receptor nuclear translocator ( Arnt ) from adult rabbits . ^^^ The deduced amino acid sequences of rabbit AhR and Arnt showed 80 % and 94 % identities with those of human AhR and Arnt , respectively . ^^^ Arnt complex could bind to the consensus xenobiotic responsive element , which indicates that AhR expressed in adult rabbit liyers possessed binding activity to the consensus xenobiotic responsive element in vitro , although aryl hydrocarbons did not induce the activity of AHH in adult rabbits . ^^^ We propose that the incapability of adult rabbits to induce cytochrome P 450 1A1 ( CYP1A1 ) is caused by factors other than AhR and Arnt . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The discovery that the oxygen regulated transcription factor HIF 1 alpha and the dioxin receptor AhR share the common heterodimerization partner ARNT ( HIF 1 beta ) raised the question whether a cross talk between oxygen and dioxin signal transduction pathways exists . ^^^ We further studied HIF 1 and AhR / ARNT DNA binding activity as well as the regulation of oxygen and xenobiotic responsive genes by treating mouse Hepa 1 hepatoma cells with hypoxia and / or the dioxin analogue ICZ . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
We have previously reported the purification of the transformed TCDD . receptor complex from rat liver cytosol based on binding to its dioxin responsive enhancer sequence ( DRE ) and that it comprises the AhR ligand binding monomer and its dimerization partner , ARNT . ^^^ When the ligand . receptor complex was bound to a DRE oligonucleotide containing BrdU and then UV irradiated , two proteins in each of the receptor preparations were found to crosslink to BrdU DRE , and we concluded that they are the AhR monomer and ARNT protein . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In an effort to better understand the mechanism of toxicity of 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin , we employed an iterative search of human expressed sequence tags to identify novel basic helix loop helix PAS ( bHLH PAS ) proteins that interact with either the Ah receptor ( AHR ) or the Ah receptor nuclear translocator ( ARNT ) . ^^^ We characterized five new `` members of the PAS superfamily , ' ' or MOPs 1 5 , that are similar in size and structural organization to the AHR and ARNT . ^^^ The expression profiles of the AHR , MOP 1 , and MOP 2 mRNAs , coupled with the observation that they all share ARNT as a common dimeric partner , suggests that the cellular pathways mediated by MOP 1 and MOP 2 may influence or respond to the dioxin signaling pathway . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon ( or dioxin ) receptor ( AhR ) is a ligand activated basic helix loop helix ( bHLH ) protein that heterodimerizes with the bHLH protein ARNT ( aryl hydrocarbon nuclear translocator ) forming a complex that binds to xenobiotic regulatory elements in target gene enhancers . ^^^ Cloning of the receptor and its DNA binding partner , ARNT , has facilitated detailed efforts to understand the mechanisms of AhR mediated signal transduction . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The arylhydrocarbon receptor nuclear translocator ( ARNT ) is a member of the basic helix loop helix PAS family of heterodimeric transcription factors which includes the arylhydrocarbon receptor ( AHR ) , hypoxia inducible factor 1alpha ( HIF 1alpha ) and the Drosophila single minded protein ( Sim ) . ^^^ In response to environmental pollutants , AHR ARNT heterodimers regulate genes involved in the metabolism of xenobiotics , whereas ARNT HIF 1alpha heterodimers probably regulate those involved in the response to oxygen deprivation . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
We examined cardiotoxicity induced by 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin ( TCDD ) in the chick embryo and the cardiac expression of transcription factors , the aryl hydrocarbon receptor ( AhR ) which binds TCDD , and its dimer partner , the AhR nuclear translocator ( Arnt ) . ^^^ To evaluate AhR and Arnt expression , untreated chick embryos were collected on Days 2 . 2 , 3 , 4 , 5 , and 8 of incubation , preserved in Bouin ' s fixative , sectioned , and stained with AhR and Arnt antibodies . ^^^ The AhR was expressed ubiquitously in cardiac myocytes , while Arnt expression was restricted to myocytes overlying developing septa : atrioventricular canal , outflow tract , and atrial and ventricular septa . ^^^ In addition , cardiac expression of an AhR / Arnt target , cytochrome P4501A1 , was restricted to myocardium coexpressing AhR and Arnt . ^^^ Thus , the spatial and temporal expression of AhR and Arnt suggests that the developing myocardium and cardiac septa are potential targets of TCDD induced teratogenicity , and such targets are also consistent with cardiac hypertrophy and septal defects observed following TCDD exposure . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In contrast , mRNA expression of the AHR partner protein Arnt ( aryl hydrocarbon receptor nuclear translocator ) was not influenced . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
A 3 , 4 benzopyrene resistant mutant clone ( c 4 ) of the mouse hepatoma Hepa 1c1c7 cell line was examined for the mutation that causes the defective function of aryl hydrocarbon receptor ( AHR ) nuclear translocator ( Arnt ) . ^^^ Arnt dimerizes with AHR and mediates the induction signal of aryl hydrocarbon hydroxylase activity . ^^^ The inability of [ Asp 326 ] Arnt / AHR heterodimers to enhance reporter gene transcription under the control of the CYP1A1 gene promoter and enhancer confirmed that the G 326 > D substitution was a causative mutation . ^^^ While fluorescence microscopy and coimmunoprecipitation experiments showed that this mutant form of Arnt was not changed from wild type Arnt in terms of nuclear localization or heterodimer formation with AHR , the binding activity of the [ Asp 326 ] Arnt 10 AHR heterodimer to the xenobiotic responsive element was reduced markedly . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Aryl hydrocarbon receptor nuclear translocator ( ARNT ) is a component of the transcription factors , aryl hydrocarbon receptor ( AhR ) and hypoxia inducible factor 1 , which transactivate their target genes , such as CYP1A1 and erythropoietin , in response to xenobiotic aromatic hydrocarbons and to low O 2 concentration , respectively . ^^^ Since ARNT was isolated as a factor required for the nuclear translocation of AhR from the cytoplasm in response to xenobiotics , the subcellular localization of ARNT has been of great interest . ^^^ We found a clear nuclear localization of ARNT in the absence of exogenous ligands to AhR , and identified the nuclear localization signal ( NLS ) of amino acid residues 39 61 . ^^^ Aryl hydrocarbon receptor nuclear translocator ( ARNT ) is a component of the transcription factors , aryl hydrocarbon receptor ( AhR ) and hypoxia inducible factor 1 , which transactivate their target genes , such as CYP1A1 and erythropoietin , in response to xenobiotic aromatic hydrocarbons and to low O 2 concentration , respectively . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The present study localizes and examines the effects of TCDD on the expression of the Ah receptor ( AhR ) , the Ah receptor nuclear translocator ( ARNT ) , epidermal growth factor ( EGF ) , and transforming growth factor alpha ( TGF alpha ) in the epithelial cells of the developing urinary tract , particularly the ureteric bud derivatives ( ureter and tubules ) . ^^^ Regardless of treatment , the expression of both AhR and ARNT increased in epithelial cells of the ureter and AhR increased in the metanephric tubules from GD 12 14 . ^^^ In situ hybridization localized the expression of AhR and ARNT mRNAs to these derivatives of the ureteric bud and levels of mRNA increased throughout the developmental period examined . ^^^ There were no significant effects of TCDD treatment on expression of AhR , while TCDD significantly decreased levels of ARNT in tubules on GD 14 . ^^^ In summary , the epithelial cells of the embryonic mouse urinary tract expressed AhR , ARNT , EGF , and TGF alpha in developmentally dependent patterns . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Western blot analysis was used to determine the concentration of the aryl hydrocarbon receptor nuclear translocator ( ARNT ) protein and aryl hydrocarbon receptor ( AHR ) in 11 mammalian cell culture lines derived from hepatic and nonhepatic tissues . ^^^ The strategy was to first use Western blot analysis to determine the expression of ARNT or AHR in each cell line relative to its concentration in murine wild type Hepa 1c1c7 ( Hepa 1 ) cells . ^^^ Actual ARNT and AHR concentrations in known amounts of total cell lysates were then determined by generating a standard curve with defined amounts of a highly purified ARNT or AHR protein and performing regression analysis . ^^^ The ratio of AHR to ARNT ranged from 0 . 3 in H4IIE cells to 10 in the Hepa 1 line with the majority of cells expressing 1 5 times more AHR than ARNT protein . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Indirect immunofluorescence analyses demonstrated the presence of aryl hydrocarbon receptor ( AHR ) and aryl hydrocarbon receptor nuclear translocator ( ARNT ) proteins in both basal and differentiating keratinocytes . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
To determine whether or not USF 1 could inhibit the interaction of aryl hydrocarbon receptor ( AhR ) / AhR nuclear translocator ( Arnt ) complex with XRE , we transfected USF 1 SR alpha expression vector into HepG 2 cells . ^^^ The results showed that no interaction of AhR / Arnt complex with XRE occurred even when the cells were treated with 2 , 3 , 7 , 8 tetrachlorodibenzofuran ( TCDF ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The AHR belongs to the basic helix loop helix / Per ARNT Sim ( bHLH PAS ) family of transcriptional regulatory proteins , whose members play key roles in development , circadian rhythmicity , and environmental homeostasis ; however , the normal cellular function of the AHR is not yet known . ^^^ Database searches and phylogenetic analyses identified four putative PAS proteins in the nematode Caenorhabditis elegans , including possible AHR and ARNT homologs . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Gel shift analysis with radiolabeled XRE and specific peptide antibodies toward AhR and aryl hydrocarbon receptor nuclear translocator protein ( Arnt ) revealed an OME mediated translocation of the AhR . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
AHR cDNAs derived from the mutant encode a protein that is unimpaired in ligand dependent dimerization with ARNT , but the AHR . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
A heterodimer of AhR ( aryl hydrocarbon receptor ) and Arnt ( AhR nuclear translocator ) conveys a transactivation signal of aromatic hydrocarbons such as 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin and 3 methylcholanthrene to the genes for a group of drug metabolizing enzymes . ^^^ This inducible expression of the genes is inhibited by adenovirus E1A , suggesting that CBP / p300 is somehow involved in the transactivation of the genes by the AhR and Arnt heterodimer . ^^^ Yeast and mammalian two hybrid systems revealed that CBP / p300 interacted with the transactivation domain of Arnt , but not with that of AhR , via the CREB binding domain . ^^^ Considering these results and that Arnt or Arnt 2 functions as a common partner in the formation of transcriptional regulators with other bHLH / PAS proteins such as AhR , HLF , and HIF 1alpha , the possibility arises that CBP / p300 is extensively involved as a coactivator in the transactivation process by bHLH / PAS ( a conserved sequence motif among Per , Arnt , and Sim ) heterodimer transcription factors through interaction with Arnt or Arnt2 . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The human aryl hydrocarbon receptor ( AHR ) and aryl hydrocarbon receptor nuclear translocator protein ( ARNT ) were coexpressed in the yeast Saccharomyces cerevisiae to create a system for the study of this heterodimeric transcription factor . ^^^ Specific transcriptional activation mediated by AHR / ARNT heterodimer , which is a functional indicator of receptor expression , was assessed by beta galactosidase activity produced from a reporter plasmid . ^^^ Yeast expressing AHR and ARNT displayed constitutive transcriptional activity that was not augmented by addition of AHR agonists in strains that required exogenous tryptophan for viability . ^^^ Tryptophan , indole , indole acetic acid , and tryptamine activated transcription in yeast coexpressing AHR and ARNT ( EC 50 values approximately 300 microM ) . ^^^ This yeast system is useful for the study of AHR / ARNT protein complexes , and may be generally applicable to the investigation of other multiprotein complexes . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The ligand activated aromatic hydrocarbon receptor ( AHR ) dimerizes with the AHR nuclear translocator ( ARNT ) to form a functional complex that transactivates expression of the cytochrome P 450 CYP1A1 gene and other genes in the dioxin inducible [ Ah ] gene battery . ^^^ Using gel mobility shift and luciferase reporter gene expression assays , we found that c 37 cells that had not been exposed to exogenous Ah receptor ligands already contained transcriptionally active AHR ARNT complexes , a finding that we also observed in wild type Hepa 1 cells treated with Ellipticine , a CYP1A1 inhibitor . ^^^ In CV 1 cells , transient expression of AHR and ARNT leads to high levels of AHR ARNT dependent luciferase gene expression even in the absence of an agonist . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Recent cDNA cloning has revealed that the AhR , like Arnt , possesses characteristic structural motifs of basic helix loop helix and PAS domains responsible for DNA recognition , heterodimerization , and ligand binding , and functions as a novel receptor type transcription factor . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Here , we describe orthologs of AHR and its dimerization partner AHR nuclear translocator ( ARNT ) in the nematode Caenorhabditis elegans , encoded by the genes ahr 1 and aha 1 , respectively . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
TCDD treatment did not alter the levels of the dimerization partner of the AHR , the AH receptor nuclear translocator protein ( ARNT ) , or ARNT mRNA . ^^^ In the presence of TCDD , both the AHR and the ARNT protein can be maintained at high levels in the nucleus if transcription is inhibited with actinomycin D . ^^^ In the absence of actinomycin D , the AHR protein was lost rapidly , but the ARNT protein level in the cell was maintained . ^^^ Together , these results suggest that the AHR protein is degraded through a selective mechanism that spares the ARNT protein and that the degradation pathway involves a protein that itself has a short half life . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Expression of functional aromatic hydrocarbon receptor and aromatic hydrocarbon nuclear translocator proteins in murine bone marrow stromal cells . 2 , 3 , 7 , 8 Tetrachlorodibenzo p dioxin ( TCDD ) acting through the aromatic hydrocarbon receptor ( AhR ) and its dimerization partner , the AhR nuclear translocator protein ( arnt ) , elicits numerous toxicological effects including immunosuppression and thymic atrophy . ^^^ The purpose of this study was to characterize AhR and arnt expression in three murine bone marrow stromal cell lines ( S 17 , M 2 10B4 , and BMS 2 ) and in primary stromal cell cultures . ^^^ These results indicate that murine bone marrow stromal cells express AhR and arnt proteins . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The basic helix loop helix PAS ( bHLH PAS ) protein ARNT is a dimeric partner of the Ah receptor ( AHR ) and hypoxia inducible factor 1 alpha ( HIF 1 alpha ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The AhR nuclear translocator ( ARNT ) protein was highly expressed in both cell lines . ^^^ In addition , the AhR and ARNT are functional in CH12 . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
There is currently little information concerning the time dependent relationship between 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin ( TCDD ) exposure and aryl hydrocarbon receptor ( AHR ) and aryl hydrocarbon receptor nuclear translocator ( ARNT ) protein concentration in vivo . ^^^ Therefore , female Sprague Dawley rats were given a single oral dose of TCDD ( 10 micrograms / kg ) , and the AHR and ARNT protein concentrations in liver , spleen , thymus , and lung determined by Western blotting . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
E 2 responsiveness of the CD / L sequence was also dependent on an adjacent overlapping GCGTG motif corresponding to the dioxin responsive element ( DRE ) core binding sequence , which is the cognate response element for the heterodimeric aryl hydrocarbon receptor ( AhR ) / AhR nuclear translocator ( ARNT ) transcription factor complex . ^^^ The results show that ER mediated transactivation of CD / L was associated with the Sp 1 ( N ) 2 4DRE ( core ) motif and involved formation of a multiprotein ER / Sp1 AhR / ARNT complex . ^^^ These results illustrate a unique example of an endogenous role for AhR / ARNT in the absence of added AhR agonist and indicate that the cathepsin D gene proximal promoter region contains at least three different functional motifs associated with ER mediated transactivation . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Studies in mutant hepatoma cells indicate that induction requires both the aromatic hydrocarbon receptor ( AhR ) and the AhR nuclear translocator ( Arnt ) . ^^^ Furthermore , induction requires AhR ' s transactivation domain , but not that of Arnt . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Xenobiotic responsive element binding protein ( XRE BP ) , a heterodimer of aryl hydrocarbon receptor ( AhR ) and its nuclear translocator ( Arnt ) , regulates the transcription of cytochrome P 450 1A1 gene ( CYP1A1 ) through XRE in response to xenobiotic inducers . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
S 1 nuclease protection assay showed that the induction of CYP1A1 mRNA by MC occurred in rabbit kidney RK 13 cells but not in rabbit lung R9ab cells , while aryl hydrocarbon receptor ( AhR ) and AhR nuclear translocator ( Arnt ) mRNAs were expressed in both cells at similar levels . ^^^ Thus , it seemed that the binding of the AhR / Arnt complex to the xenobiotic responsive element ( XRE ) was inhibited by the hypermethylation of CpG dinucleotides within an XRE core sequence ( 5 ' CGTG 3 ' ) . ^^^ To examine whether or not the binding of the AhR / Arnt heterodimer to XRE is affected by the methylation status of XRE , a gel shift assay using a methylated XRE as a probe was carried out . ^^^ As expected , the AhR / Arnt complex could not bind to the methylated XRE . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Because TCDD toxicity could possibly be the result of alterations in gene transcription mediated by the TCDD / aryl hydrocarbon receptor ( AhR ) / AhR nuclear translocator ( ARNT ) complex , the presence of the AhR and ARNT in the various organs of the adult male reproductive tract was examined using Western blotting . ^^^ Although technical difficulties precluded the immunohistochemical evaluation of AhR distribution in these organs , ARNT was localized in all organs in a variety of cell types , including germ cells , epithelial cells , fibroblasts , smooth muscle cells , and endothelial cells . ^^^ Finally , AhR and ARNT expression in TCDD exposed and control animals was evaluated by Western blotting . ^^^ Results revealed no effect of TCDD exposure on ARNT protein expression , while AhR expression was decreased to 5 51 % of control in all organs examined . ^^^ In summary , both AhR and ARNT were expressed in all organs of the adult male rat reproductive tract examined , and epithelial and / or endothelial cells within each of these organs ( with the exception of the testis ) were responsive to TCDD exposure in terms of CYP1A1 induction . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Because one mechanism by which TCDD is thought to cause toxicity is by aryl hydrocarbon receptor ( AhR ) mediated alterations in gene transcription , the goals of this study were to determine whether the developing prostate expresses the AhR and its dimerization partner , the AhR nuclear translocator ( ARNT ) ; to determine whether in utero and lactational TCDD exposure is capable of directly activating gene transcription in the developing prostate ; and to identify prostatic mRNAs that exhibit altered abundance in response to in utero and lactational TCDD exposure . ^^^ Using reverse transcriptase polymerase chain reaction ( RT PCR ) , mRNAs encoding the AhR and ARNT were detected in both ventral and dorsolateral prostates from control animals throughout postnatal development . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Expression of a functional heterodimeric transcription factor composed of human aryl hydrocarbon receptor ( Ahr ) and aryl hydrocarbon receptor nuclear translocator ( Arnt ) proteins was accomplished using a single pBEVY plasmid . ^^^ Previous studies suggest that inhibitory cross talk between the estrogen receptor and the Ahr / Arnt complex may occur and that Hsp 90 Ahr complex formation is important for Ahr mediated signal transduction . ^^^ No inhibitory cross talk was observed in signaling assays performed with yeast that co expressed Ahr , Arnt and estrogen receptor . ^^^ In contrast , Ahr / Arnt mediated signal transduction was reduced by 80 % in a temperature sensitive Hsp 90 strain grown under non permissive conditions . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The results suggest that differential expression of both AhR and ARNT are responsible for rat strain specific differences in TCDD induced CYP1A1 expression . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In this report , we examined the levels of AhR protein and its associated protein ( Arnt ) during the adipose differentiation in 3T3 L 1 cells . ^^^ However , the pattern of the depletion of the Arnt protein was distinct from that of the AhR protein . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Arnt 2 is a member of the bHLH PAS ( Per , Ahr , Arnt , Sim ) family of transcription factors and shares the highest similarity with Arnt . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Aryl hydrocarbon receptor ( AhR ) nuclear translocator ( Arnt ) gene has been isolated and characterized from a mouse genomic DNA library . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Expression of the aryl hydrocarbon receptor ( AhR ) and the aryl hydrocarbon receptor nuclear translocator ( ARNT ) in fetal , benign hyperplastic , and malignant prostate . ^^^ BACKGROUND : Androgen dependent tissue has been reported to be affected by chemical ligands of the aryl hydrocarbon receptor ( AhR ) , a ligand activated transcription factor , which heterodimerizes with the aryl hydrocarbon receptor nuclear translocator protein ( ARNT ) . ^^^ RESULTS : Immunoblotting of enriched prostatic epithelial cells ( EC ) and stromal cells revealed constitutive expression of bands at around 110 kDa and 90 kDa , using anti AhR and anti ARNT , respectively . ^^^ Constitutive expression of AhR ( primarily cytoplasmic ) and ARNT ( nuclear and cytoplasmic ) by the majority of adult basal and secretory EC , CaP , and smooth muscle cells was confirmed in situ . ^^^ The most intense anti AhR / ARNT reactivity was found on smooth muscle cells , followed by EC and fibrocytes . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor ( AHR ) and its DNA binding partner , the AHR nuclear translocator ( ARNT ) , are basic helix loop helix transcription factors that mediate many of the toxic and carcinogenic effects of polyhalogenated aromatic hydrocarbons . ^^^ The basic regions of the AHR and ARNT contact the GCGTG recognition site , whereas both their helix loop helix domains and periodicity ARNT single minded domains participate in heterodimerization . ^^^ To delineate the transcription factors that may facilitate DNA binding and transcriptional activation of the AHR / ARNT heterodimer , we questioned whether transcription factor IIB ( TFIIB ) may interact with either the AHR or ARNT and whether this interaction may affect the ability of the AHR / ARNT complex to bind DNA . ^^^ Coaffinity precipitation assays demonstrated that both the AHR and ARNT were capable of interacting with TFIIB . ^^^ Domain mapping experiments revealed that TFIIB interacts with the periodicity ARNT single minded and carboxyl terminal regions of the AHR . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Immunohistochemical staining patterns for AHR , aryl hydrocarbon nuclear translocator ( ARNT ; the heterodimerization partner of AHR ) , and two AHR regulated genes , Cyp1A1 and Cyp1B1 , were similar and not altered by treatment of mammary glands in organ culture with TCDF . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The expression of mRNAs encoding the regulators of CYP 1 enzymes the AHR and its heterodimerization partner , the ARNT ( AH receptor nuclear translocator ) protein was not altered by treatment with TCDD or MC . ^^^ However , the cytosolic content of AHR protein and ARNT protein was depleted substantially following treatment with TCDD . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Protein and mRNA for epidermal growth factor ( EGF ) , transforming growth factor alpha ( TGF alpha ) , EGF receptor , transforming growth factor beta 1 ( TGF beta 1 ) , TGF beta 2 , TGF beta 3 , glucocorticoid receptor ( GR ) , the aryl hydrocarbon receptor ( AhR ) , and the Ah receptor nuclear translocator ( ARNT ) were localized in gestational days ( GD ) 49 59 human embryonic secondary palates . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The AhR is a ligand activated transcription factor belonging to the basic helix loop helix / Per AhR Arnt Sim ( bHLH / PAS ) superfamily of proteins . ^^^ The mechanism of induction of gene transcription by TCDD involves ligand recognition and binding by the AhR , nuclear translocation , and dimerization with the AhR cofactor , AhR nuclear translocator ( Arnt ) . ^^^ Although Arnt is a necessary component of a functional nuclear AhR complex , this protein also forms transcriptionally active heterodimers with other bHLH / PAS factors , including those involved in the transcriptional response to hypoxia . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Therefore , this study examined the daily cycle of AhR and AhR nuclear translocator ( Arnt ) protein expression in multiple tissues of female Sprague Dawley rats . ^^^ The apparent daily cycle of AhR and Arnt protein expression in multiple tissues was not observed within the spleen . ^^^ This preliminary report is the first study to suggest that the PAS proteins , AhR and Arnt , exhibit a daily oscillation pattern within multiple target tissues which may give insight into the tissue specific toxic and biochemical responses mediated through this dimerization pair , as well as the physiological function of these proteins . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
AHR is a member of the Per , ARNT , Sim / basic helix loop helix superfamily of ligand activated transcription factors that also harbors the transcription factors involved in the hypoxia response , development of the central nervous system , and day night adaptations . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
These studies characterized the profile of AhR and ARNT expression in primary splenocytes and purified splenic B cells after cellular activation with lipopolysaccharide ( LPS ) . ^^^ LPS treatment of mouse splenocytes markedly increased the magnitude of both AhR and ARNT steady state mRNA expression . ^^^ Western blotting also demonstrated an increase in the relative magnitude of both the AhR and ARNT proteins in LPS activated splenocytes . ^^^ Likewise , the presence of the AhR , ARNT and cytochrome P450IA1 ( CYP1A1 ) proteins were also detected in purified primary splenic B cells , and the magnitude of protein expression was enhanced in LPS activated splenic B cells at 12 and 24 hr relative to time matched controls for each of these proteins . ^^^ In summary , these findings suggest that on LPS activation the magnitude of AhR and ARNT mRNA and protein increases in both splenocytes and purified primary splenic B cells . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In liver , ALDH 3 is inducible by polycyclic aromatic hydrocarbon xenobiotics by an Ah receptor ( AhR ) mediated pathway in which a liganded AhR complexes with nuclear ARNT protein , and the complex binds to a xenobiotic response element ( XRE ) sequence located near 3 . 0 kb in the ALDH 3 5 ' flanking region and initiates transcription . ^^^ Western analysis confirms the presence of AhR and ARNT proteins in 3 methylcholanthrene treated rat liver , as well as ARNT protein in rat corneal epithelium . ^^^ The 3 . 0 kb ALDH 3 XRE region contains multiple overlapping transcription factor binding sequences , including consensus sites for AhR , ARNT , HNF 1 , HNF 4 , and C / ebp . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In addition to induction of drug metabolizing enzymes , the liganded AhR complex was found to activate gene expression of a factor designated AhR repressor ( AhRR ) , which inhibits AhR function by competing with AhR for dimerizing with Arnt and binding to the XRE sequence . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
C 2 ceramide also induced the apoptosis of an AHR containing cell line lacking the aryl hydrocarbon receptor nuclear translocator protein . ^^^ These results suggest the AHR modulates aspects of ceramide signaling associated with the induction of apoptosis but not cell cycle arrest , and does so by a mechanism that is independent of its interaction with aryl hydrocarbon receptor nuclear translocator and exogenous AHR ligands . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The AHR and its dimerization partner ARNT are members of the emerging bHLH PAS family of transcriptional regulatory proteins . ^^^ An overview of the bHLH PAS family is presented in relation to the diversity of bHLH PAS proteins and the functional and evolutionary relationships of the AHR and ARNT to the other members of this family . ^^^ Finally , some of the most promising directions for future research on the comparative biochemistry and molecular biology of the AHR and ARNT are discussed . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
There was no apparent difference in the mass of the AHR nuclear translocator protein ( ARNT ) between the two strains , but the hepatic concentration of ARNT was about three times as high in L E as in H / W rats . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
AhR , ARNT , and CYP1A1 mRNA quantitation in cultured human embryonic palates exposed to TCDD and comparison with mouse palate in vivo and in culture . 2 , 3 , 7 , 8 Tetrachlorodibenzo p dioxin ( TCDD ) is developmentally toxic in many species and induces cleft palate in the C57BL / 6N mouse embryo . ^^^ The present study quantitates the expression of AhR , ARNT , and CYP1A1 mRNA in human embryonic palates in organ culture . ^^^ In human palates , AhR expression correlated with ARNT and CYP1A1 mRNA expression . ^^^ The ratio of AhR to ARNT was approximately 4 : 1 . ^^^ AhR mRNA increased during the culture period in both treated and control subjects ; however , ARNT expression was relatively constant . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
RT PCR quantification of AHR , ARNT , GR , and CYP1A1 mRNA in craniofacial tissues of embryonic mice exposed to 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin and hydrocortisone . ^^^ The present study quantifies AhR , AhR nuclear translocator ( ARNT ) , and GR mRNA at 4 , 12 , 24 , and 48 h after exposure ( time 0 = dose administration at 8 A . ^^^ The relative overall expression level of the genes was AhR > ARNT > GR . ^^^ Within individuals , expression of AhR and / or ARNT was highly correlated with GR level . ^^^ AhR , ARNT , and GR mRNA are upregulated in early palatogenesis , although not on the same schedule . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Cytochrome P 450 ( CYP ) 1B1 expression in mouse hepatoma ( Hepa 1 ) wild type ( WT ) cells was compared with responses in Hepa 1 variants LA 1 and LA 2 , which , respectively , exhibit low aryl hydrocarbon receptor ( AhR ) level and defective AhR nuclear translocator ( ARNT ) protein . 10T1 / 2 mouse embryo fibroblasts express predominantly CYP1B1 and at a 100 times higher level than in Hepa 1 cells , whereas they express about 300 fold lower CYP1A1 than Hepa 1 cells . ^^^ Two CYP1B1 enhancer elements [ xenobiotic responsive element ( XRE ) 1 / 2 and XRE 4 ] formed TCDD unresponsive complexes of similar mobility to TCDD stimulated AhR ARNT complex with XRE 5 . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Effects of stage of development and 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin ( TCDD ) exposure on aryl hydrocarbon receptor ( AhR ) and AhR nuclear translocator ( ARNT ) protein concentrations in reproductive organs of male rats were determined . ^^^ The developmental decreases in prostatic AhR and ARNT protein were associated with decreases in AhR and ARNT mRNA . ^^^ AhR and ARNT protein concentrations in fetal urogenital sinus on Gestation Days ( GD ) 16 , 18 , and 20 were similar to levels in ventral prostate on PND 7 . ^^^ TCDD exposure of adult male rats ( 0 . 2 , 1 , 5 , or 25 micrograms / kg po , 24 h ) decreased AhR but not ARNT protein in ventral and dorsolateral prostate , vas deferens , and epididymis . ^^^ In utero and lactational TCDD exposure ( 1 . 0 micrograms / kg dam po , GD 15 ) did not alter ARNT levels but reduced prostatic AhR protein levels on PND 7 and delayed the developmental decrease in AhR protein in ventral and dorsolateral prostate . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The expression levels of the aryl hydrocarbon receptor ( AhR ) , the aryl hydrocarbon receptor nuclear translocator ( ARNT ) , and AR were not affected by exposure to TCDD at a dose of 10 nM for a 24 hr time period . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In contrast , AhR in TCDD treated cells was primarily immunoprecipitated from nuclear extracts and was associated with Arnt but not 90 kDa heat shock protein . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
While expression of CYP1A1 mRNA was not detected and levels of ARNT mRNA were not altered by TCDD exposure , the amount of AhR mRNA was decreased dose dependently . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor ( AHR ) and the alpha class hypoxia inducible factors ( HIF1alpha , HIF2alpha , and HIF3alpha ) are basic helix loop helix PAS ( bHLH PAS ) proteins that heterodimerize with ARNT . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Two regulatory proteins , the aromatic ( aryl ) hydrocarbon receptor ( AhR ) and the AhR nuclear translocator ( Arnt ) , mediate induction . ^^^ AhR and Arnt are prototypical members of the basic helix loop helix / Per Arnt Sim class of transcription factors . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Aromatic hydrocarbon nuclear translocator ( Arnt ) is an ubiquitously expressed protein that contains basic helix loop helix ( bHLH ) and Per AhR Arnt Sim ( PAS ) motifs . ^^^ Arnt has been identified as a heterodimerization partner for AhR . ^^^ AhR / Arnt heterodimer binds the regulatory region of xenobiotic induced genes and activates their transcription . ^^^ Given that the expression of Arnt is limited , HIF 1alpha may compete with AhR for recruiting Arnt as a heteromeric partner . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Aryl hydrocarbon receptor ( AhR ) levels , but not AhR nuclear translocator protein ( ARNT ) levels were highly dependent on cell type ; AhR was high and ER independent in MDA , and low and ER linked in T47D . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The AhR nuclear translocator ( ARNT ) is involved in AhR signal transduction . ^^^ We have analyzed the expression of AhR and ARNT mRNA and AhR protein in Day 3 pc ( postcoitum ) rabbit morulae and Days 4 and 6 pc blastocysts using RT PCR , nested PCR , whole mount in situ hybridization , and whole mount immunohistochemistry with subsequent confocal laser scanning analysis . ^^^ AhR and ARNT transcripts were detected in all stages investigated , indicating coexpression of both transcription factors . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The AhR , AhR nuclear translocator ( Arnt ) , and AhR / Arnt proteins were coimmunoprecipitated with 35S ERAP 140 and 35S SMRT and , in gel mobility shift assays , AhR / Arnt binding to 32P dioxin response element ( DRE ) was enhanced by ERAP 140 and inhibited by SMRT ; supershifted bands were not observed . ^^^ These results confirmed functional and physical interactions of AhR / Arnt with ERAP 140 and SMRT in breast cancer cells . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The sequence of events following the binding of the AhR / AhR nuclear translocator protein ( ARNT ) heterodimer to dioxin response elements has yet to be completely understood . ^^^ The role of coactivator , RIP 140 , in the modulation of transcriptional activity of AhR / ARNT heterodimer was examined . ^^^ Co immunoprecipitation and co localization assays revealed that RIP 140 interacted with AhR , but not with ARNT , both in vitro and in cells . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Dioxin and other aryl hydrocarbons bind to the PAS domain of Ahr , causing Ahr to translocate to the nucleus , where it dimerizes with another bHLH PAS protein , the aryl hydrocarbon receptor nuclear translocator ( Arnt ) . ^^^ Ahr : Arnt heterodimers then activate transcription of target genes that encode enzymes involved in metabolizing aryl hydrocarbons . ^^^ Coexpression of Ss and Tgo in Drosophila SL 2 cells causes transcriptional activation of reporters containing mammalian Ahr : Arnt response elements , indicating that Ss : Tgo heterodimers are very similar to Ahr : Arnt heterodimers in DNA binding specificity and transcriptional activation ability . ^^^ This suggests that the interaction of Ss and Tgo does not require additional signals , unlike the ligand dependent interaction of Ahr and Arnt . ^^^ Despite the very different biological roles of Ahr and Arnt in insects and mammals , the molecular mechanisms by which these proteins function appear to be largely conserved . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
All AhRs were expressed with their species specific AhR nuclear translocator ( ARNT ) in COS 7 cells . ^^^ Transactivation activity was determined for two PCDD , two PCDF , and seven PCB congeners with each of the four AhR / ARNT pairs using prt1Aluc , a luciferase reporter driven by two dioxin responsive enhancer elements ( DREs ) from the rainbow trout cyp1A gene . ^^^ Of the four AhR / ARNT pairs tested with PCDD , PCDF , and non ortho PCB congeners , three exhibited high activity ( rainbow trout AhR2alpha , zebrafish AhR 2 , and human AhR ) , while rainbow trout AhR2beta had very weak or no activity . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The region of residues 145 to 119 ( CD / L ) of the cathepsin D gene promoter contains a GC rich motif that binds Sp 1 protein and an adjacent pentanucleotide ( CACGC ) that corresponds to the core sequence of a dioxin responsive element ( DRE ) and binds the aryl hydrocarbon receptor ( AhR ) AhR nuclear translocator ( Arnt ) complex . ^^^ The requirements for AhR Arnt interactions with Sp 1 protein for maximal activity of pCD / L were confirmed in wild type MCF 7 and Hepa 1c1c7 cells and Arnt deficient Hepa 1c1c7 cells using antisense Arnt and Arnt expression plasmids . ^^^ The functional interactions of Sp 1 with AhR Arnt were paralleled by physical interactions showing that AhR Arnt and Sp 1 proteins were co immunoprecipitated and AhR Arnt enhanced Sp 1 [ ( 32 ) P ] CD / L binding in electrophoretic mobility shift assays . ^^^ The physical and functional interactions of Sp 1 with AhR Arnt proteins bound to the Sp 1 ( N ) ( 4 ) DRE ( core ) motif were also dependent on the proximity of these sites , and both the activity and the extent of Sp 1 DNA binding decreased as the number of intervening nucleotides increased from 4 to 20 . ^^^ These studies show that regulation of basal expression of some genes by Sp 1 may also require interactions with AhR Arnt . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Protein levels of AhR and aryl hydrocarbon receptor nuclear translocator ( Arnt ) were similar in A 427 and CL 3 cells before B [ a ] P treatment . ^^^ Since AhR and / or Arnt function is impaired in A 427 cells , this cell line offers a model for investigating the repression mechanisms of CYP1A1 induction by B [ a ] P in lung cells . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The arylhydrocarbon receptor ( AhR ) and the arylhydrocarbon receptor nuclear translocator ( ARNT ) are members of the PAS gene family mediating toxic effects of xenobiotics such as dioxin and polychlorinated biphenyls . ^^^ We have analyzed the expression and cellular distribution of rabbit AhR and ARNT mRNA and protein level in the nonpregnant uterus and the pregnant and pseudopregnant uterus at Days 6 to 12 of gestation . ^^^ Upon attachment of the blastocyst at Day 7 of gestation , a strong expression of AhR and ARNT mRNA was observed in the luminal and glandular epithelium of the antimesometrial uterine compartment . ^^^ In contrast , AhR and ARNT expression was low in the luminal epithelium of the paraplacental and the mesometrial placental fold . ^^^ During early placentation at Day 9 of gestation , expression of AhR and ARNT was first observed in the perivascular decidualized stromal cells and , at Day 12 , extended to the decidualized stromal cells of the placental bed . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The protein chaperone heat shock protein 90 ( Hsp 90 ) is a major regulator of different transcription factors such as MyoD , a basic helix loop helix ( bHLH ) protein , and the bHLH Per aryl hydrocarbon nuclear translocator ( ARNT ) Sim ( PAS ) factors Sim and aryl hydrocarbon receptor ( Ahr ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
I cells with 10 nM 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin ( TCDD ) and 81 nM phorbol 12 myristate 13 acetate ( PMA ) , an activator of sn 1 , 2 diacylglyerol binding PKCs , enhanced transactivation of the reporter construct several fold relative to cells treated with a saturating 10 nM TCDD dose alone ; this effect was dubbed the `` PMA effect . ' ' A domain swapping and deletional analysis of the native AhR and AhR nuclear translocator ( ARNT ) protein transactivation domains ( TADs ) was performed to determine if these domains are absolutely required for the AhR 10 ARNT dimer mediated PMA effect in the L10 . ^^^ I model for these analyses and that endogenous AhR and ARNT levels are extremely low in this model . ^^^ Transient coexpression of the AhR and ARNT 474 FLAG , an ARNT protein lacking the native ARNT TAD , in L10 . ^^^ I cells reveals the native ARNT TAD is not absolutely required for the AhR 10 ARNT 474 FLAG dimer to mediate the PMA effect . ^^^ Transient coexpression of AhRDeltaCVP , a chimeric AhR protein in which the native AhR TAD has been replaced with the VP 16 ( herpes simplex virus protein 16 ) TAD ( which control experiments demonstrate is unaffected by PMA ) , and ARNT in L10 . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Importantly , gel mobility shift analysis and Western blotting showed that the same level of AHR / ARNT complexes could be detected in cells treated with 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin during hypoxia and normoxia . ^^^ These data suggest that the effects of hypoxia on AHR mediated gene regulation occur distal to the formation of AHR / ARNT complexes and imply that functional interference between hypoxia and AHR mediated signaling does not occur through competition for ARNT protein . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Once bound by ligand , the AhR interacts with the AhR nuclear translocator ( ARNT ) protein to form the aryl hydrocarbon receptor complex ( AHRC ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
It has been hypothesized that after ligand binding , the AhR dissociates from its chaperone complex and translocates into the nucleus , where it heterodimerizes with its DNA binding partner , the AhR nuclear translocator ( ARNT ) , and activates specific genes . ^^^ In addition , we demonstrate that AhR / ARNT dimerization , but not nuclear translocation of the AhR , is inhibited by molybdate . ^^^ This indicates that 1 ) HSP 90 dissociation is not required for nuclear translocation of the AhR , 2 ) HSP 90 dissociation is essential for formation of the AhR / ARNT heterodimer , and 3 ) an additional undefined regulatory step is required for AhR / ARNT dimerization in the nucleus . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor ( AHR ) / AHR nuclear translocator ( ARNT ) heterodimer interacts with naturally occurring estrogen response elements . ^^^ We then examined a second possible mechanism , i . e . direct inhibition of ER alpha binding to estrogen response elements ( EREs ) by the AHR / AHR nuclear translocator ( ARNT ) complex . ^^^ The AHR / ARNT heterodimer did not bind either a full or half site ERE . ^^^ However , AHR / ARNT bound specifically to oligomers containing naturally occurring EREs derived from the human c fos , pS 2 , and progesterone receptor ( PR ) gene promoters that include xenobiotic response element ( XRE ) like sequences . ^^^ We propose that at least part of the mechanism by which the AHR / ARNT complex inhibits estrogen action is by competitively inhibiting ER alpha binding to imperfect ERE sites , adjacent to or overlapping XREs . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In contrast , the AHR nuclear translocator protein ( ARNT ) did not interact with COUP TF , ERRalpha 1 , or ERalpha . ^^^ Purified ERalpha did not bind the consensus XRE , but COUP TFI bound the consensus XRE , suggesting a role for COUP TF as a AHR / ARNT competitor for XRE binding . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Arnt complex and `` superinduces ' ' the expression of endogenous CYP1A1 gene by TCDD , indicating that the proteasomal degradation of AhR serves as a mechanism for controlling the activity of the activated receptor . ^^^ We also show that deletion of the transcription activation domain of AhR abolishes the degradation , whereas a mutation in the DNA binding region of AhR or Arnt reduces the degradation ; these data implicate the transcription activation domain and DNA binding in AhR degradation . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
To address this , we examined the presence of messenger RNAs of arylhydrocarbon receptor ( AhR ) , AhR nuclear translocator ( Arnt ) and two dioxin responsive genes , cytochrome P 450 1B1 ( CYP1B1 ) and downstream of tyrosine kinases ( p 62 ( dok ) ) , in the endometrium of women with or without endometriosis using semi quantitative reverse transcription polymerase chain reaction . ^^^ In contrast , the expression levels of AhR , Arnt and CYP1B1 seemed to be constant during the cycle . ^^^ In terms of the comparison between non endometriosis and endometriosis group , the mRNA levels of AhR , Arnt , CYP1B1 and p 62 ( dok ) were essentially similar . ^^^ Based on the regression analysis , significant linear and positive correlations were observed between AhR and Arnt mRNA levels , and between Arnt and p 62 ( dok ) mRNA levels . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Estrous cycle dependent changes in the expression of aromatic hydrocarbon receptor ( AHR ) and AHR nuclear translocator ( ARNT ) mRNAs in the rat ovary and liver . ^^^ The aromatic hydrocarbon receptor ( AHR ) and AHR nuclear translocator protein ( ARNT ) mediate the toxic effects of a wide variety of halogenated and polycyclic aromatic hydrocarbons . ^^^ The present study seeks to examine the regulation of AHR and ARNT mRNAs in liver and ovarian tissues across the rat estrous cycle . ^^^ Message for hepatic AHR was increased significantly on the morning of proestrus , and decreased dramatically by the evening of proestrus ; while hepatic ARNT mRNA was significantly decreased between diestrus and the morning of proestrus , and between the evening of proestrus and the morning of estrus . ^^^ These data suggest that while estrogen may not be the key regulator of AHR mRNA expression , a factor associated with the rat reproductive cycle may be important in regulating the expression of both the AHR and ARNT genes in the ovary and liver . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor ( AhR ) is a ligand activated transcription factor that acts in concert with the AhR nuclear translocator ( ARNT ) , and alters gene expression in response to environmental contaminants such as 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin ( TCDD ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Furthermore , cycloheximide is shown to increase the accumulation of the TCDD activated AhR and the functional AhR 10 Arnt complex in nucleus . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Since hypoxia inducible factor 1alpha ( HIF 1alpha ) and the arylhydrocarbon receptor ( AhR ) shared the AhR nuclear translocator ( Arnt ) for hypoxia and AhR mediated signaling , respectively , it was possible to establish the hypothesis that hypoxia could regulate cytochrome P 450 1a1 ( Cyp1a1 ) expression . ^^^ Thus , we might suggest that hypoxia inhibits TCDD induced Cyp1a1 expression due to the competition between HIF 1alpha and the AhR for the Arnt in Hepa 1 cells . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Treatment of Ishikawa cells with the AhR agonist [ 3H ] 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin ( TCDD ) gave a radiolabeled nuclear complex that sedimented at 6 . 0 S in sucrose density gradients , and Western blot analysis confirmed that Ishikawa cells expressed human AhR and AhR nuclear translocator ( Arnt ) proteins . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
ARNT is the central partner of several heterodimeric transcription factors , including those containing the aryl hydrocarbon ( dioxin ) receptor ( AhR ) and the hypoxia inducible factor 1alpha ( HIF1alpha ) . ^^^ Our results show that the TEL ARNT fusion protein is the crucial product of the translocation and suggest that interference with the activity of AhR or HIF1alpha can contribute to leukemogenesis . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Blockage of nuclear export in HepG 2 cells with leptomycin B ( LMB ) resulted in increased levels of AHR AHR nuclear translocator ( ARNT ) complex in the nucleus and correlative reductions in agonist stimulated AHR degradation . ^^^ Mutagenesis of leucines 66 and 71 of the putative AHR NES resulted in a protein with reduced function in dimerization to ARNT and binding to DNA , while alanine substitution at leucine 69 ( AHR ( A 69 ) ) resulted in an AHR that bound with ARNT and associated with DNA . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In in vitro interaction assays , SRC 1 was found to interact with AhR but not with ARNT . ^^^ Examination of the role of SRC 1 in AhR / ARNT transactivation in ARNT deficient mutant Hepa 1 c 4 cells demonstrates that the AhR transactivation domain is sufficient for enhanced coactivation mediated by SRC 1 in the presence of a transactivation domain deleted ARNT protein . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
We investigated the effects of 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin ( TCDD ) , in prepubertal ( PP ) and adult ( A ) rat granulosa cells ( GC ) in vitro by examining the changes in estrogen secretion , aromatase enzyme activity and mRNAs for steroidogenic enzymes P450scc , 3beta HSDI , P450arom ; and for components of the AHR signaling pathway CYP1A1 , aromatic hydrocarbon receptor ( AHR ) , and the AHR nuclear translocator protein ( ARNT ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
To determine the role of ARNT in gene control in the intact animal mouse liver , expression of target genes under control of an ARNT dimerization partner , the aryl hydrocarbon receptor ( AHR ) , was monitored . ^^^ Induction of CYP1A1 , CYP1A2 , and UGT1 * 06 mRNAs by the AHR ligand 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin was absent in livers of Arnt floxed / Mx1 Cre mice treated with polyinosinic polycytidylic . ^^^ These data demonstrate that ARNT is required for AHR function in the intact animal . ^^^ Despite more than 80 % loss of the ARNT expression in lung , maximal induction of CYP1A1 was found , indicating that the expression level of ARNT is not limiting to AHR signaling . ^^^ These studies establish a critical role for ARNT in AHR and HIF 1alpha signal transduction in the intact mouse . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Therefore , we used a sensitive in situ hybridization histochemical ( ISHH ) method to map the neural expression of AhR mRNA , as well as those of the mRNAs encoding the AhR dimerization partners , arylhydrocarbon receptor nuclear translocator ( ARNT ) and ARNT 2 . ^^^ We found that AhR , ARNT , and ARNT 2 mRNAs were widely distributed throughout the brain and brainstem . ^^^ There was no neuroanatomic evidence that AhR is preferentially colocalized with ARNT or ARNT 2 . ^^^ The most noteworthy regions in which we found AhR , ARNT , and ARNT 2 mRNA were several hypothalamic and brainstem regions involved in the regulation of appetite and circadian rhythms , functions that are disrupted by dioxin exposure . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
To date , results from the literature indicate that induction of CYP1A1 does not only involve the classical activation cascade of the Ah receptor , e . g . binding of the ligand to the AhR , heterodimerisation with Arnt protein , constitution of a complex with XRE responsive element and subsequent gene activation . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
This increase is not observed in the aromatic hydrocarbon receptor ( AhR ) defective TAOc1BP ( r ) c 1 and the AhR nuclear translocator ( Arnt ) defective BP ( r ) c 1 variants , demonstrating that the induction of mdr 1 by 3 MC requires AhR . ^^^ Arnt , also activated p 53 and induced mdr 1 transcription . 2 , 3 , 7 , 8 Tetrachlorodibenzo p dioxin , an AhR ligand resistant to metabolic breakdown , had no effect . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The expression of cytochrome P 450 1A1 ( CYP1A1 ) , the aryl hydrocarbon receptor ( AHR ) , and the aryl hydrocarbon receptor nuclear translocator ( ARNT ) were analyzed in different brain regions and pituitaries using semiquantitative RT PCR and Western blotting . ^^^ The AHR and ARNT mRNAs were detected in the same areas but in contrast to CYP1A1 the changes in AHR and ARNT mRNA expression were limited to the 28 day time point . ^^^ The present results provide evidence for the presence of CYP1A1 , AHR , and ARNT in the central nervous system and in the pituitary , suggesting that TCDD may exert a direct effect on these regions . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Estimation of the size of the transcripts for AhR and ARNT protein revealed normal sizes , 2 . 7 and 2 . 4 kb , respectively . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
An uncommon phenotype of poor inducibility of CYP1A1 in human lung is not ascribable to polymorphisms in the AHR , ARNT , or CYP1A1 genes . ^^^ Induction requires the aryl hydrocarbon receptor ( AHR ) and aryl hydrocarbon receptor nuclear translocator ( ARNT ) proteins . ^^^ All seven lung samples expressed normal levels of AHR mRNA and ARNT mRNA , indicating that they did not carry inactivating polymorphisms in the 5 ' upstream regulatory regions of these genes . ^^^ Sequencing of cDNAs encompassing the complete coding regions of AHR and ARNT identified a previously known codon 554 polymorphism in AHR , which was present in the homozygous state in one individual . ^^^ Previously unreported silent single nucleotide polymorphisms were identified in codon 44 of AHR and codon 189 of ARNT . 1500 bp of genomic sequence from the 5 ' upstream regulatory sequence of the CYP1A1 gene was also sequenced in the non inducible individuals . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Gel shift assays revealed that the PST rich domain affected AHR mediated signaling by inhibiting DNA binding of the AHR * ARNT heterodimer . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Gel retardation experiments showed that proteasome inhibition induced transcriptionally active AHR ARNT heterodimers able to bind to a consensus xenobiotic responsive element . ^^^ Synchronized to AHR activation , proteasome inhibition also induced a transient increase in AHR nuclear translocator ( ARNT ) at the protein and mRNA levels . ^^^ Since nuclear levels of AHR and ARNT are relevant for AHR transcriptional activation , our data suggest that proteasome inhibition , through a transient increase in ARNT expression , could promote AHR stabilization and accumulation into the nuclear compartment . ^^^ An elevated content of nuclear AHR could favor AHR ARNT heterodimers able to bind to xenobiotic responsive elements and to induce gene transcription in the absence of xenobiotics . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The expression of aryl hydrocarbon receptor ( AhR ) and AhR nuclear translocator ( Arnt ) mRNAs is seen even in adult rabbits . ^^^ The AhR / Arnt mediated activation of XRE TK / Luc reporter gene in RK 13 cells is blocked by transfection with a USF 1 expression vector . ^^^ These results indicate that the XRE of the rabbit CYP1A1 gene is recognized by the basic helix loop helix proteins to regulate the expression of CYP1A1 in both an agonistic ( AhR / Arnt ) and an antagonistic ( USF 1 ) manner . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The steady state levels of expression of aryl hydrocarbon receptor ( AhR ) and aryl hydrocarbon receptor nuclear translocator ( ARNT ) were similar in all three cell lines . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor nuclear translocator ( ARNT ) is a basic helix loop helix transcription factor that forms heterodimers with the aryl hydrocarbon receptor ( AhR ) or hypoxia inducible factor 1alpha to activate transcription via xenobiotic response element or hypoxia response element , respectively . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The nuclear AhR complex is a heterodimer containing the AhR and AhR nuclear translocator ( Arnt ) proteins , and the molecular mechanism of AhR action is associated with binding of the heterodimer to dioxin responsive elements ( DREs ) in regulatory regions of Ah responsive genes . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Short heterodimer partner ( SHP ) orphan nuclear receptor inhibits the transcriptional activity of aryl hydrocarbon receptor ( AHR ) / AHR nuclear translocator ( ARNT ) . ^^^ SHP acts as a negative regulator of these receptors by inhibiting DNA binding and transcriptional activation . 2 , 3 , 7 , 8 Tetrachlorodibenzo p dioxin ( TCDD ) binds to arylhydrocarbon receptor ( AHR ) , activating the AHR / AHR nuclear translocator ( ARNT ) heterodimer . ^^^ We investigated the physical and functional interaction of SHP with AHR / ARNT . ^^^ In GST pull down assays , ARNT interacted directly with SHP in vitro , but AHR did not interact with GST SHP . ^^^ SHP inhibited AHR / ARNT DNA binding in vitro . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The ubiquitously expressed basic helix loop helix ( bHLH ) PAS protein ARNT ( arylhydrocarbon receptor nuclear transporter ) forms transcriptionally active heterodimers with a variety of other bHLH PAS proteins , including HIF 1alpha ( hypoxia inducible factor 1alpha ) and AHR ( arylhydrocarbon receptor ) . ^^^ The closely related protein ARNT 2 is highly expressed in the central nervous system and kidney and also forms complexes with HIF 1alpha and AHR . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
AHR function may be regulated by structural variations in AHR itself , in the AHR repressor ( AHRR ) , in the AHR nuclear translocator ( ARNT ) , or in AHR target molecules such as cytochrome P 4501A1 ( CYP1A1 ) and glutathione S transferase . ^^^ No association was found between uterine endometriosis and any polymorphisms in the AHRR , AHR , ARNT , or CYP1A1 genes analyzed in the present study . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Finally , elevation in CYP1A1 mRNA levels was not observed when mutant Hepa 1c1c7 cells , which are defective in either the aryl hydrocarbon receptor ( AhR ) or aryl hydrocarbon receptor nuclear translocator ( ARNT ) , were treated with AGN 193109 . ^^^ This suggests that the AhR / ARNT pathway and not the RAR / RXR pathway is mediating the elevation of CYP1A1 mRNA levels by AGN 193109 , at least in the Hepa 1c1c7 cells . ^^^ This is the first example of a retinoid that displays the abililty to regulate both the RAR / RXR and AhR / ARNT transcriptional regulatory pathways . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Expression of AhR and ARNT mRNA in cultured human endometrial explants exposed to TCDD . ^^^ The present study characterizes the expression of AhR and ARNT mRNA in a human endometrial explant culture model in the absence and presence of TCDD exposure . ^^^ After culture , AhR and ARNT mRNA expression were quantified by RT PCR . ^^^ TCDD treatment significantly increased the expression of AhR mRNA , but not ARNT mRNA . ^^^ The expression of both genes was similar for all individual explants and the ratio of AhR : ARNT mRNA expression across all samples was 1 . 7 to 1 . 8 . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor nuclear translocator ( ARNT ) mediates aryl hydrocarbon signaling and toxicity by dimerizing with the ligand activated aryl hydrocarbon receptor ( AHR ) , forming a complex that binds specific DNA elements and alters transcription of target genes . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Mechanistic studies reveal an AhR / DRE paradigm for the induction , which involves activation of the aryl hydrocarbon receptor ( AhR ) by an agonist , dimerization of AhR with the Ah recceptor nuclear translocator ( Arnt ) , followed by binding of the AhR / Arnt heterodimer to the dioxin responsive enhancer ( DRE ) and transcription of the gene . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In this study we show that rat GC express mRNA for AHR and the AHR nuclear translocator ( ARNT ) as well as biomarkers of TCDD action , CYP1A1 and CYP1B1 mRNA . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
When the AhR and ARNT were co transfected together in Y 1 cells or human adrenocortical carcinoma H295R cells , the promoter activity of the StAR gene significantly ( P < 0 . 05 ) increased , to a level 1 . 4+ / 0 . 01 fold higher in Y 1 cells and to a level 1 . 6+ / 0 . 04 fold higher in H295R cells than the control level , when 1 microM of betaNF was added . ^^^ We examined the effect of induction of cAMP with transfection with AhR or ARNT . ^^^ With the addition of 1 mM 8 Br cAMP , there were no differences between the StAR gene promoter activities in the group in which AhR and ARNT was introduced and in the group in which they were not introduced . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The 5 ' flanking region ( approximately 1 . 0 kb ) of the cyp19a gene had TATA ( nt 48 ) and CAAT ( nt 71 ) boxes , a steroidogenic factor 1 ( SF 1 ) binding site ( nt 265 ) , eight copies of the SRY / SOX motif , and two copies of a recognition site for binding the arylhydrocarbon receptor ( AhR ) / AhR nuclear translocator factor ( ARNT ) heterodimer . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The diet induced changes in CYP 1 enzyme expression were consistent with changes in the AhR and ARNT transcription factors that regulate them . ^^^ Decreased ( P < 0 . 05 ) mammary constitutive AhR and ARNT proteins were measured in SPI fed rats . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor ( AhR ) is a ligand activated transcription factor that acts in concert with the AhR nuclear translocator ( ARNT ) . ^^^ Furthermore , this mutation decreases the transcriptional activity of the AhR / ARNT system , which is likely due to the suppression of AhR / ARNT / XRE complex formation . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Uterine and ovarian aryl hydrocarbon receptor ( AHR ) and aryl hydrocarbon receptor nuclear translocator ( ARNT ) mRNA expression in benign and malignant gynaecological conditions . ^^^ The transcriptional regulators aryl hydrocarbon receptor ( AHR ) and aryl hydrocarbon receptor nuclear translocator ( ARNT ) modulate the transcription of genes involved in cellular differentiation and proliferation . ^^^ AHR and ARNT mRNA transcripts were readily detected by a ribonuclease protection assay in all reproductive tissues examined . ^^^ However , post menopausal women on continuous hormone replacement therapy had greater expression of AHR but not of ARNT in the endometrium and myometrium when compared with women not taking hormones . ^^^ Leiomyomas expressed significantly less AHR and ARNT mRNA compared with normal myometrium . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor ( AHR ) , after binding xenobiotic ligands such as 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin ( TCDD ) , associates with the AHR nuclear translocator ( ARNT ) , and the dimer so formed activates transcription of several genes , including the cytochrome P4501A1 ( CYP1A1 ) gene . ^^^ We show that BRG 1 potentiates AHR / ARNT mediated reporter gene activity in a TCDD dependent fashion in Hepa1c1c7 cells . ^^^ Introduction of BRG 1 into the BRG 1 and hBrm deficient SW 13 and C33A human cell lines also enhances expression from a transiently transfected AHR / ARNT dependent reporter gene . ^^^ Chromatin immunoprecipitation analysis demonstrated that BRG 1 associates with the enhancer region of the mouse CYP1A1 gene in vivo in a TCDD and ARNT dependent fashion , suggesting the specific recruitment of BRG 1 by AHR / ARNT . ^^^ Together these studies reveal a functional involvement of BRG 1 in activating CYP1A1 gene transcription and implicate the importance of ATP dependent chromatin remodeling activity on inducible gene expression mediated by AHR / ARNT . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
AhR is a cytosolic ligand dependent transcription factor that , in conjunction with the AhR nuclear translocator ( Arnt ) , binds to dioxin response elements ( DREs ) located in the promoter region of target genes , such as CYP1A1 , and induces their transcription . ^^^ The sum of the data demonstrates that some synthetic retinoids in addition to activating the retinoic acid receptor / retinoid 10 receptor pathway are capable of binding to AhR and activating the AhR / Arnt pathway . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The effects of dioxins are mediated via the aryl hydrocarbon receptor ( AHR ) and its binding protein , aryl hydrocarbon receptor nuclear translocator ( ARNT ) . ^^^ In mice , aryl hydrocarbon receptor repressor ( AHRR ) , which binds to ARNT in competition with AHR , plays a critical negative regulatory role in AHR signaling . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The cyp19a promoter region has TATA and CAAT boxes , a steroidogenic factor 1 ( SF 1 ) binding site , and two aryl hydrocarbon receptor ( AhR ) / AhR nuclear translocator factor ( ARNT ) binding motifs . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
No quantitative differences in the expression of AhR and its partner transcription factor ARNT were seen in low and high passage number PC 3 and DU 145 cells in the absence and presence of TCDD as assessed by RT PCR and Western blotting . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor ( AhR ) and its nuclear translocator ( Arnt ) are dispensable for normal mammary gland development but are required for fertility . ^^^ The aryl hydrocarbon receptor ( AhR ) and its nuclear translocator ( Arnt ) are transcription factors that play a role in the detection of and adaptation to environmental signals . ^^^ To distinguish defects autonomous to mammary epithelium from indirect effects controlled by ovarian hormones , we transplanted Arnt null and AhR null mammary epithelium into wild type mice and evaluated development after one pregnancy . ^^^ Normal mammary structures were observed in the absence of Arnt and AhR , demonstrating that neither transcription factor is necessary for mammary development . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor complex heterodimeric transcription factor , comprising the basic helix loop helix Per ARNT Sim ( bHLH PAS ) domain aryl hydrocarbon receptor ( AHR ) and aryl hydrocarbon receptor nuclear translocator ( ARNT ) proteins , mediates the toxic effects of TCDD ( 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin ) . ^^^ In this report , we demonstrate that SRC 1 , NCoA 2 , and p / CIP are capable of independently enhancing TCDD dependent induction of a luciferase reporter gene by the AHR / ARNT dimer . ^^^ We demonstrate by coimmunoprecipitation and by a reporter gene assay that SRC 1 and NCoA 2 but not p / CIP are capable of interacting with ARNT in vivo after transient transfection into mammalian cells , while AHR is capable of interacting with all three coactivators . ^^^ We confirm the interactions of ARNT and AHR with SRC 1 with immunocytochemical techniques . ^^^ SRC 1 does not require its bHLH PAS domain to interact with ARNT or AHR , but utilizes distinct domains proximal to its p300 / CBP interaction domain . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor nuclear translocator ( ARNT ) plays an essential role in vertebrate transcriptional regulation as the common subunit of transcriptionally active complexes like the aryl hydrocarbon receptor ( AHR ) / ARNT heterodimer and hypoxia inducible factor 1 , mediating cellular responses to certain xenobiotics and to hypoxia , respectively . ^^^ Although no significant correlation with activities of CYP1A2 , which is under regulatory control of the AHR / ARNT transcription complex , could be established , metabolic or pathological phenotypes may be associated with these variations . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Expression of the mediators of dioxin toxicity , aryl hydrocarbon receptor ( AHR ) and the AHR nuclear translocator ( ARNT ) , is developmentally regulated in mouse teeth . ^^^ The aryl hydrocarbon receptor ( AHR ) together with the AHR nuclear translocator ( ARNT ) protein is believed to mediate the toxic effects of dioxins . ^^^ To study the potential involvement of the AHR ARNT pathway in the dental toxicity of TCDD , we analysed the expression of AHR and ARNT by in situ hybridization and immunohistochemistry in developing mouse teeth . ^^^ The coexpression of AHR and ARNT during early tooth development as well as during the information and mineralization of the dental matrices is suggestive of the AHR ARNT pathway as a mediator of dental toxicity of TCDD . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
However , both proteins failed to form functional complexes with mammalian or rainbow trout AHR nuclear translocator protein ( ARNT ) that could associate with xenobiotic response elements ( XREs ) in a ligand dependent manner in vitro . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
A Hepa1c1c7 wild type cell [ H 1 ( wt ) ] , an aryl hydrocarbon receptor ( AhR ) deficient mutant [ H 1 ( AhR ( ) ) ] and an AhR nuclear translocator ( Arnt ) deficient mutant [ H 1 ( Arnt ( ) ) ] were used for this study . ^^^ TCDD induced PAI 1 in H 1 ( wt ) cells , but not in H 1 ( AhR ( ) ) and H 1 ( Arnt ( ) ) mutants , indicating a functional role of the AhR Arnt complex in this effect . ^^^ Transfection with PAI 1 promoter led to increased PAI 1 promoter activity in H 1 ( wt ) cells treated with TCDD , but no such effect occurred in H 1 ( AhR ( ) ) or H 1 ( Arnt ( ) ) cells , implying involvement of the AhR and Arnt . ^^^ In summary , TCDD induced the PAI 1 gene directly via an AhR and Arnt dependent mechanism , which was distinctly different from TGF beta driven PAI 1 transcription . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In addition , the aryl hydrocarbon receptor ( AhR ) and AhR nuclear translocator ( ARNT ) were down and up regulated by TCDD , respectively , indicating that AhR mediated signal transduction was altered in the ovary . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The AhR complex is a ligand dependent transcription factor that binds to its specific DNA recognition site as a dimer with the AhR nuclear translocator ( ARNT ) and activates gene transcription . ^^^ Association of SMRT with the AhR : ARNT : DNA complex was not detected by GST pull down or gel retardation assays . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Genetic analyses reveal that induction is abolished in aromatic hydrocarbon receptor ( AhR ) or aromatic hydrocarbon receptor nuclear translocator ( Arnt ) defective variants but restored upon reconstitution of the variant cells with cDNAs expressing functional AhR or Arnt . ^^^ Moreover , induction is largely reduced in cells expressing a deletion mutant of AhR or Arnt lacking the transcription activation ( TA ) domain , thus implicating the TA activities of both AhR and Arnt in the induction . ^^^ These findings establish that induction of TiPARP by TCDD is mediated through an AhR and Arnt transcription activation dependent signal transduction that is repressed by a labile factor through the ubiquitin 26S proteasome mediated protein degradation . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
To better understand the role of the aryl hydrocarbon receptor ( AHR ) signaling pathway in causing tissue specific signs of 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin ( TCDD ) toxicity in zebrafish , the temporal and spatial expression of the zebrafish aryl hydrocarbon receptor 2 ( zfAHR 2 ) , aryl hydrocarbon receptor nuclear translocator 2 ( zfARNT 2 ) , and an AHR regulated gene , cytochrome P4501A ( zfCYP1A ) , were assessed in larvae exposed to vehicle or TCDD ( 1 . 55 nM ) from 3 4 h postfertilization ( hpf ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Hsp 90 appears to be involved in the initial folding of newly synthesized AhR , stabilization of ligand binding conformation of the receptor , and inhibition of constitutive dimerization with ARNT . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
DNA binding and protein interactions of the AHR / ARNT heterodimer that facilitate gene activation . ^^^ Gene activation by the aryl hydrocarbon receptor ( AHR ) and its DNA binding partner , the aryl hydrocarbon receptor nuclear translocator ( ARNT ) requires a number of sequential steps that occur following the binding of ligand and entry of the AHR into the nuclear compartment . ^^^ This includes heterodimerization of the AHR and ARNT , formation of the appropriate amino acid / nucleotide contacts at the GCGTG recognition site and interactions between either the AHR or ARNT with proteins that facilitate changes in chromatin structure . ^^^ The majority of these steps are likely modulated by changes in both phosphorylation and oxidation status of the AHR , ARNT and associated proteins . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Estrous cycle dependent expression of AHR , AHR nuclear translocator ( ARNT ) and ER mRNAs in the rat ovary was reported . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In all treatments the concentration of the AHR nuclear translocator ( ARNT ) protein was unchanged and had no impact on the localization of the AHR . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
For instance , the expression of CYP 1 genes can be induced via the aryl hydrocarbon receptor ( AhR ) which dimerizes with the AhR nuclear translocator ( ARNT ) , in response to many polycyclic aromatic hydrocarbon ( PAHs ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The expression of CYP1A1 and CYP1B1 is regulated by the aromatic hydrocarbon receptor / Ah receptor nuclear translocator ( AhR / ARNT ) transcription factor complex . ^^^ Trx 1 transfected MCF 7 cells show increased AhR / ARNT DNA binding activity that is not due to altered AhR or ARNT protein expression . 2 , 3 , 7 , 8 Tetrachlorodibenzo p dioxin ( TCDD , dioxin ) induced expression of CYP1B1 in MCF 7 cells is increased by Trx 1 . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Down regulation of the expression of the Arnt by RNAi diminished the effects of the AhR on the cell proliferation of the A 549 cells . ^^^ Consequently , we conclude that the AhR , presumably in collaboration with the Arnt , activates the DNA synthesis and the subsequent cell proliferation in A 549 cells . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The arylhydrocarbon receptor ( AhR ) , but not the AhR nuclear translocator ( ARNT ) , is increased in hearts of patients with cardiomyopathy . ^^^ The objective of this study was to investigate the expression of the arylhydrocarbon receptor ( AhR ) and its partner AhR nuclear translocator ( ARNT ) in left ventricle specimens from explanted hearts from patients with cardiomyopathy ( CMP ) . ^^^ Expression of AhR and ARNT was analyzed using semi quantitative immunohistochemistry , and in selected samples , Western blot and reverse transcription polymerase chain reaction analysis were performed to confirm AhR and ARNT expression . ^^^ In all groups , the intensity of anti ARNT staining was more pronounced than AhR staining , but significant differences or any age related alterations were not observed . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon ( Ah ) receptor ( AhR ) is a member of the basic helix loop helix PER ARNT SIM ( PAS ) transcription factor family . ^^^ In addition , AhR complexes may affect cellular signaling through interactions with various other regulatory and signaling proteins , including PAS heterodimerization partners ( ARNT ) , chaperone and immunophilin like proteins ( e . g . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The involvement of the aryl hydrocarbon receptor ( AHR ) and aryl hydrocarbon nuclear translocator ( ARNT ) in mediating the effects of oltipraz on the XRE is supported by electrophoretic mobility supershift data and AHR / ARNT overexpression studies . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Correlation between gene expression of aryl hydrocarbon receptor ( AhR ) , hydrocarbon receptor nuclear translocator ( Arnt ) , cytochromes P4501A1 ( CYP1A1 ) and 1B1 ( CYP1B1 ) , and inducibility of CYP1A1 and CYP1B1 in human lymphocytes . ^^^ The relationships between gene expression of aryl hydrocarbon receptor ( AhR ) , aryl hydrocarbon receptor nuclear translocator ( Arnt ) , cytochromes P4501A1 ( CYP1A1 ) , 1B1 ( CYP1B1 ) , CYP1A1 , and the inducibility of CYP1A1 and CYP1B1 were determined in 32 cultivated human lymphocytes . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
TCDD related toxicity is considered mainly to be mediated by the aryl hydrocarbon receptor ( AHR ) protein , which binds TCDD , and heterodimerizes with its partner protein , the aryl hydrocarbon receptor nuclear translocator ( ARNT ) , and binds to xenobiotica responsive elements ( XREs ) in the promoter regions of biotransformation genes as well as genes involved in growth , differentiation and cellular homeostasis . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Limited digestion of [ ( 35 ) S ] AhR / AhR nuclear translocator ( Arnt ) by trypsin produced a peptide of approximately 40 kDa that was more resistant to proteolysis in the presence of 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin ( TCDD ) than vehicle and was also Arnt dependent . ^^^ This trypsin resistant peptide was also elicited in the presence of other agonist ligands , but not with antagonist ligands that do not form the DNA binding AhR / Arnt complex . ^^^ Immunoblot of trypsin treated AhR / Arnt + / TCDD indicated that the trypsin resistant peptide did not include the N terminal portion of the AhR against which the antibody was made . ^^^ We conclude that agonist ligands initiate structural alteration in AhR that is Arnt dependent and at least partially involves the ligand binding / Per Arnt Sim domain . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The liganded AhR translocates from cytoplasm to nuclei where it switches its partner molecule from Hsp 90 to Arnt . ^^^ Thus formed AhR / Arnt heterodimer binds a specific DNA sequence designated XRE in the promoter region of the target genes including CYP1A1 , UDP glucuronosyl transferase and others to enhance their expression . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Dioxins , e . g . 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin ( TCDD ) , use the aryl hydrocarbon receptor ( AHR ) / aryl hydrocarbon receptor nuclear translocator ( ARNT ) receptor complex to mediate their toxic actions . ^^^ In addition to interaction with environmental pollutants , several transcription factors , steroid receptors , and growth factors are capable interacting with the AHR / ARNT complex , which suggests a constitutive role for the receptor complex . ^^^ Our experiments revealed a complex distribution of AHR and ARNT mRNAs and proteins in rat and human testis . ^^^ AHR and ARNT immunoreactivities could be detected in the nuclei of interstitial and tubular cells . ^^^ We suggest that AHR and ARNT mediate effects by direct action on testicular cells in the rat and human testis . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
These findings imply that the proteolytic machinery present in the cytoplasmic compartment is sufficient to degrade the AHR and that nuclear translocation , binding with ARNT , or DNA binding are not necessary for efficient degradation of the AHR . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor ( AhR ) is a ligand dependent transcription factor that requires heterodimerization with its partner , the Ah receptor nuclear translocator ( Arnt ) , for activation of transcription . ^^^ This AhR / Arnt / DRE complex has been well characterized and can be observed readily by the gel shift assay . ^^^ Human AhR and Arnt with a C terminal histidine tag have been expressed functionally using a baculovirus expression system . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The dependence of the AhR in this differential interaction was further demonstrated using in vitro synthesized guinea pig and mouse Ah receptors and mouse Arnt . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor ( AhR ) is a member of the basic helix loop helix PAS ( Per ARNT SIM ) transcription family , which also includes hypoxiainducible factor 1alpha ( HIF 1alpha ) and its common dimerization partner AhR nuclear translocator ( ARNT ) . ^^^ Following ligand activation or hypoxia , AhR or HIF 1alpha , respectively , translocate into the nucleus , dimerize with ARNT , and regulate gene expression . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
For regulation of gene activities , AHR dimerizes with another member of the bHLH / PAS protein family , AHR nuclear translocator ( ARNT ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
To our knowledge , this is the most comprehensive investigation to date designed to evaluate the key genes in the pathway , including AhR , aryl hydrocarbon receptor nuclear translocator , CYP1A1 and CYP1B1 transcripts and CYP1A1 associated 7 ethoxyresorufin O deethylase ( EROD ) activity in a population heavily exposed to dioxin . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Transfection of siRNAs for the aryl hydrocarbon receptor ( AhR ) and AhR nuclear translocator ( ARNT ) mRNAs in MCF 7 breast cancer cells resulted in a 60 to 80 % decrease in levels of AhR and ARNT proteins in whole cell extracts and decreased binding of nuclear extracts to 32P labeled dioxin responsive element . siRNA for the AhR also decreased 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin ( TCDD ) induced CYP1A1 protein , CYP1A1 dependent activity , and luciferase activity in cells transfected with an Ah responsive construct . 17beta estradiol ( E 2 ) induces proliferation of MCF 7 cells through enhanced G0 / G1 > S phase progression , and this response is inhibited in cells cotreated with E 2 plus TCDD . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
A heterodimer of the dioxin receptor ( AhR ) and Arnt , which are basic helix loop helix / PAS family transcription factors , mediates most of the toxic effects of dioxins . ^^^ Here we show that the agonist activated AhR / Arnt heterodimer directly associates with oestrogen receptors ER alpha and ER beta . ^^^ Our findings suggest a novel mechanism by which ER mediated oestrogen signalling is modulated by a co regulatory like function of activated AhR / Arnt , giving rise to adverse oestrogen related actions of dioxin type environmental contaminants . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
OBJECTIVE : The aryl hydrocarbon receptor repressor ( AhRR ) protein may dimerize with the AhR nuclear translocator ( ARNT ) and may compete with the aryl hydrocarbon receptor ( AhR ) to bind the xenobiotic responsive elements . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Epithelial cells in the oviduct and vagina and steroid synthesizing cells in the rabbit ovary express AhR and ARNT . ^^^ The AhR and its dimerization partner AhR nuclear translocator ( ARNT ) were found in embryos , fetuses and in genital tract tissue . ^^^ In the current study , AhR and ARNT expression and co localization were studied immunohistochemicaly during the pre implantation period in various genital tract organs ( ovary , oviduct , vagina ) of the rabbit . ^^^ The ARNT protein was found in all AhR expressing cells except for oocytes within primordial follicles . ^^^ Therefore , the full complement for AhR / ARNT transcriptional activity was found in the studied organs . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Heterodimers of bHLH PAS protein fragments derived from AhR , AhRR , and Arnt prepared by co expression in Escherichia coli : characterization of their DNA binding activity and preparation of a DNA complex . ^^^ AhR ( aryl hydrocarbon receptor ) , AhRR ( AhR repressor ) , and Arnt ( AhR nuclear translocator ) are members of the bHLH ( basic helix loop helix ) PAS ( Per AhR / Arnt Sim homology sequence ) transcription factor superfamily . ^^^ They associate with each other to form heterodimers , AhR / Arnt or AhRR / Arnt , and bind the XRE ( xenobiotic responsive element ) sequences in the promoter regions of the target genes to regulate their expression . ^^^ In the present study , we prepared heterodimeric bHLH PAS domains derived from AhR and Arnt , and AhRR and Arnt by co expressing each pair in E . coli , and showed that the heterodimers formed exhibited full DNA binding activity , which was not apparently affected by deletion of the highly basic amino acid cluster most N terminal as to the HLH region of AhR or AhRR . ^^^ Methylation of the two CpG sites in the XRE core sequence reduced the binding affinity to heterodimeric proteins , with 5 methylcytosine in the AhR recognition site exhibiting a greater inhibitory effect than that in the Arnt recognition site . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Finally , genetic experiments using AhR ( aryl hydrocarbon receptor ) , Arnt ( aryl hydrocarbon receptor nuclear translocator ) or Nrf 2 deficient cells reveal that , while induction of NQO 1 by TCDD depends on the presence of AhR and Arnt , the basal and inducible expression of NQO 1 by either TCDD or tBHQ requires functional Nrf 2 . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
We suggested that proteasome inhibition dependent AhR activation could involve an increase in the expression of the partner protein dioxin receptor nuclear translocator ( ARNT ) . ^^^ Since ARNT over expression induced nuclear translocation of the AhR , and ARNT deficient cells were unable to translocate this receptor to the nucleus upon proteasome inhibition , we have analyzed the effect of proteasome inhibition on the expression of regulatory proteins controlling ARNT levels . ^^^ Sp 1 phosphorylation and binding to the ARNT promoter , ARNT over expression and AhR nuclear translocation were inhibited by GF109203X , a protein kinase C specific inhibitor . ^^^ These data suggest that cellular control of protein kinase C activity , through Sp 1 and ARNT , could regulate AhR transcriptional activity in the absence of xenobiotics . . ^^^ The dioxin receptor ( AhR ) , in addition to its role in xenobiotic induced carcinogenesis , appears to participate in cell proliferation , differentiation and organ homeostasis . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The arylhydrocarbon receptor nuclear translocator ( ARNT ) is a member of the basic helix loop helix , PER ARNT SIM family of heterodimeric transcription factors , and serves as a dimerization partner for arylhydrocarbon receptor ( AHR ) and hypoxia inducible factor 1alpha . ^^^ However , unlike normal mice in which exposure to the environmental pollutant 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin ( TCDD ) , an AHR ligand , resulted in thymic involution , the thymus of Lck Cre ; Arnt ( flox / Delta ) mice were resistant to TCDD treatment in vivo . ^^^ In contrast , benzo ( a ) pyrene , another AHR ligand , still caused thymic involution in Lck Cre ; Arnt ( flox / Delta ) mice . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In an attempt to understand the molecular basis for the lack of inducibility of CYP1A protein expression and activity in Elizabeth River killifish , we analyzed the expression of CYP1A and four other members of the AHR signal transduction pathway : AHR 1 , AHR 2 , AHR repressor ( AHRR ) , and AHR nuclear translocator ( ARNT ) . ^^^ No population or treatment related differences were observed in expression of AHR 1 or ARNT mRNAs . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Although the expression of aryl hydrocarbon receptor ( AHR ) as well as the partner molecule , AHR nuclear translocator ( ARNT ) in the brain has been reported , the effect of TCDD on the brain remains to be clarified in detail . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Both SIM 1 and AHR require heterodimerization either with ARNT or ARNT 2 to function . ^^^ Here , we characterize the promoter for Sim 1 and show that a consensus AHR ARNT / 2 binding site positively regulates its activity in the context of transfection experiments in Neuro 2A cells . ^^^ A gel shift assay indicated that AHR ARNT / 2 can bind its putative site in the Sim 1 promoter . ^^^ Overexpression of Arnt , Arnt 2 , or Ahr increased the activity of a reporter construct containing the Sim 1 promoter by 1 . 8 , 1 . 5 , and 2 . 2 fold , respectively , but failed to do so when the AHR ARNT / 2 binding site was mutated . ^^^ We conclude that Sim 1 expression is regulated by AHR ARNT / 2 . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
To investigate the expression of aryl hydrocarbon receptor repressor ( AhRR ) and related molecules in various tissues and the effects of aromatic hydrocarbons ( AHs ) on their expression , we developed a reliable technique of quantification of human AhRR as well as aryl hydrocarbon receptor ( AhR ) , AhR nuclear translocator ( ARNT ) and cytochrome P 450 1A1 ( CYP1A1 ) mRNA by real time TaqMan PCR method . ^^^ On the other hand , CYP1A1 expression was extremely high in lung , and AhR and ARNT were ubiquitously expressed in almost all tissues . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Effects of TCDD exposure on immunolocalization of the zebrafish aryl hydrocarbon receptor nuclear translocator ( ARNT 2 ) , the heterodimer partner of the aryl hydrocarbon receptor ( AHR 2 ) , and an AHR regulated gene cytochrome P 450 1A ( CYP1A ) was also determined . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
We report here that the C . elegans orthologs of AHR and ARNT , ahr 1 and aha 1 , regulate GABAergic motor neuron fate specification . ^^^ The AHR 1 aryl hydrocarbon receptor and its co factor the AHA 1 aryl hydrocarbon receptor nuclear translocator specify GABAergic neuron cell fate in C . elegans . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
ARNT is also a dimeric partner for the Ah receptor ( AHR ) , and this complex is essential in regulating the adaptive metabolic response to polycyclic aromatic hydrocarbons . ^^^ Because of the essential role of ARNT in hypoxia driven developmental events , it has been difficult to study the physiological significance of AHR . ^^^ To address this issue , we developed a hypomorphic Arnt allele that displayed normal development and allowed the examination of the role of ARNT in AHR biology . ^^^ Liver perfusion studies demonstrated that ARNT hypomorphs have a patent ductus venosus , identical to that observed in the Ahr null mice . ^^^ Taken in sum , these experiments demonstrate that ARNT is an essential component of AHR developmental signaling and shed light on the mechanism of dioxin toxicity . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor ( AhR ) is a ligand activated transcription factor that forms a functional heterodimeric complex with the AhR nuclear translocator ( Arnt ) protein . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Replacement of tyrosine 9 with the structurally conservative phenylalanine ( AhRY9F ) abolished binding to dioxin response element ( DRE ) D , E , and A and abrogated DRE driven gene induction mediated by the AhR with no effect on TCDD binding , TCDD induced nuclear localization , or ARNT heterodimerization . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The expression of genes in the AhR dependent pathway , including AhR , aryl hydrocarbon receptor nuclear translocator ( ARNT ) , CYP1A1 , and CYP1B1 transcripts , and the CYP1A1 associated 7 ethoxyresorufin O deethylase ( EROD ) activity was measured in lymphocytes . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Expression of aryl hydrocarbon receptor ( AHR ) and aryl hydrocarbon receptor nuclear translocator ( ARNT ) mRNA expression in human spermatozoa . ^^^ BACKGROUND : The objective of this study was to determine if human spermatozoa express AHR ( dioxin receptor ) and ARNT and if mRNA levels for these transcription factors correlate with sperm concentration , motility or morphology . ^^^ MATERIAL / METHODS : This was a case controlled cohort study in which AHR and ARNT mRNA levels in subjects with normal and abnormal semen analysis were compared . ^^^ AHR / ARNT mRNA levels were determined by multiplex RT PCR using Glyceryl aldehyde 3 phospho dehydrogenase ( G3PDH ) as an internal control . ^^^ RESULTS : Human sperm expresses abundant amounts of AHR and ARNT mRNA . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The enhancer does not contain the invariant core sequence of XREs that are binding sites for the Ah receptor ( AhR ) and Arnt heterodimer . ^^^ The enhancer did not show any inducible expression in Hepa 1 derived cell lines , C 4 and C 12 , deficient of Arnt and AhR , respectively . ^^^ On the other hand , bacterially expressed AhR Arnt heterodimer could not bind to the enhancer . ^^^ Bacterially expressed AhR Arnt heterodimer interacted with the factor . ^^^ Collectively , these results demonstrate that a novel induction mechanism is present in which the AhR Arnt heterodimer functions as a coactivator . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Together with its heterodimeric partner ARNT , the ligand bound AHR binds conserved xenobiotic response elements ( XREs ) near the promoter of CYP1A and other genes . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
AhR and HLF heterodimerize with Arnt as the common partner , and bind to asymmetric E boxes termed XRE and HRE , respectively . ^^^ We expressed AhR Arnt and HLF Arnt in Escherichia coli and used them for DNA binding . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The heterodimeric complex of aromatic hydrocarbon receptor ( AHR ) and Ah receptor nuclear translocator ( ARNT ) plays a pivotal role in controlling the expression of drug metabolism genes , such as the cytochromes p 450 ( Cyp ) 1a1 and 1b1 , believed to be responsible for most toxic effects of the environmental contaminant 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin ( TCDD ) . ^^^ In this study , we show that activation of Jun N terminal kinase ( JNK ) and extracellular signal regulated kinase ( ERK ) modulates ARNT transcription activity and potentiates the transcriptional activity of AHR / ARNT complexes . ^^^ These data suggest that JNK and ERK modulate ARNT activity and AHR / ARNT dependent gene expression , contributing to the gene specific and tissue specific toxicity of environmental contaminants . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Upon ligand binding , the aryl hydrocarbon receptor ( AhR ) translocates into the nucleus and dimerizes with its partner aryl hydrocarbon receptor nuclear translocator ( Arnt ) . ^^^ The AhR Arnt heterodimer binds to the dioxin response element ( DRE ) to regulate target gene expression . ^^^ Using baculovirus expressed human AhR and Arnt , we showed that the formation of the ligand dependent AhR Arnt DRE complex requires protein factors in vitro . ^^^ The aim of this study was to determine whether two other Hsp 90 associated proteins present in rabbit reticulocyte lysate ( RRL ) , namely CyP 40 and Hsp 70 , play any role in forming the AhR Arnt DRE complex . ^^^ In contrast , CyP 40 is involved in forming the complex since ( 1 ) immunodepletion of CyP 40 from a RRL fraction reduces the intensity of the AhR Arnt DRE complex by 48 % and ( 2 ) recombinant human CyP 40 alone causes the formation of this complex . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
AhR , ARNT , and CYP1B1 were only weakly expressed , even after 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin induction , when rat mammary epithelial cells ( RMEC ) were cultured on plastic . ^^^ RMEC cultured on the extracellular matrix ( ECM ) , Matrigel , or on a floating gel of collagen 1 demonstrated branching morphogenesis and substantially increased basal CYP1B1 and induced CYP1A1 expression , in parallel with large increases in AhR and ARNT expression . ^^^ Increased AhR and ARNT expression is observed within 24 h of dispersal on Matrigel , substantially prior to branch formation . ^^^ Culture on thin layers of collagen 1 , collagen 4 , and laminin , respectively , failed to reproduce the branching morphogenesis or increases in AhR , ARNT , or CYP expression . ^^^ A synthetic peptide that selectively antagonizes integrin ECM interactions reduced branch formation , without diminishing AhR , ARNT , and CYP expression . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
TCDD bound AhR translocates to the nucleus and forms a heterodimer with its partner protein , AhR nuclear translocator ( Arnt ) . ^^^ The AhR / Arnt heterodimer then binds to the dioxin response elements ( DREs ) in the cyp1a1 enhancer and stimulates transcription of cyp1a1 . ^^^ PD 98059 pretreatment also inhibited TCDD induced DRE binding of the AhR / Arnt heterodimer . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor ( AHR ) is a ligand activated transcription factor that dimerizes with ARNT to mediate responses to compounds such as 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin ( TCDD ) . ^^^ The most well characterized target gene for the AHR / aryl hydrocarbon receptor nuclear translocator ( ARNT ) dimer is a cytochrome P 450 , CYP1A . ^^^ To determine whether CYP1A is the important downstream effector enzyme for the AHR / ARNT pathway , we used morpholino oligonucleotides ( MOs ) to block induction of CYP1A in response to TCDD in zebrafish embryos . ^^^ We conclude that the important target for the AHR / ARNT pathway in developing zebrafish exposed to TCDD is not zfcyp1a . ^^^ This suggests an alternative model in which TCDD activated AHR / ARNT disrupts the normal process of growth and development by altering programs of gene expression or function . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Our identification of 36 putatively AHRE 2 regulated genes highlights the regulatory versatility of the aryl hydrocarbon receptor ( AHR ) and the ability of the AHR and its dimerization partner , ARNT , to act both as a ligand activated transcription factor ( on AHRE 1 ) and as a ligand activated coactivator ( on AHRE 2 ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
There is ample evidence that aryl hydrocarbon receptor ( AHR ) , AHR nuclear translocator ( ARNT ) , and cytochrome P 450 1A1 ( CYP1A1 ) are involved in TCDD metabolism . ^^^ We assessed whether there is any association in the Japanese population of nonsyndromic oral clefts with single nucleotide polymorphisms ( SNPs ) in the AHR , ARNT , and CYP1A1 genes using transmission disequilibrium test ( TDT ) and case control study . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor ( AHR ) and AHR nuclear translocator ( ARNT ) are DNA binding transcription factors with basic helix loop helix / Per Arnt Sim ( bHLH PAS ) domains . ^^^ We report here that CoCoA also binds the bHLH PAS domains of AHR and ARNT and functions as a potent primary coactivator for them ; i . e . ^^^ CoCoA does not require p 160 coactivators for binding to and serving as a coactivator for AHR and ARNT . ^^^ Moreover , reduction of CoCoA mRNA levels by small interfering RNA inhibited the transcriptional activation by AHR and ARNT . ^^^ Our data support a physiological role for CoCoA as a transcriptional coactivator in AHR / ARNT mediated transcription . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Interestingly , even though the DNA sequence identifies it as the sole member of the new CYP2S family , CYP2S1 exhibits many features typical to CYP 1 family members , e . g . dioxin inducibility mediated by the aryl hydrocarbon receptor ( AHR ) and the aryl hydrocarbon receptor nuclear translocator ( ARNT ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor nuclear translocator / hypoxia inducible factor ( ARNT / HIF 1 beta ) mediates an organism ' s response to various environmental cues , including those to chemical carcinogens , such as 2 , 3 , 7 , 8 tetrachlorodibenzo rho dioxin ( TCDD or dioxin ) , via its formation of a functional transcription factor with the ligand activated aryl hydrocarbon receptor ( AHR ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In the present study , experiments in rat hepatoma cells using dominant negative DNA binding defective AhR and Ah receptor nuclear translocator ( Arnt ) mutants provided evidence that TCDD induced AhR mediated G ( 1 ) arrest is only partially regulated by direct AhR transcriptional activity , suggesting that both coactivation and corepression are involved . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Most , if not all , TCDD induced pathology is mediated via binding to the aryl hydrocarbon receptor ( AhR ) , a ligand activated transcription factor that belongs to the basic helix loop helix / Per Arnt Sim ( bHLH / PAS ) superfamily . ^^^ Upon ligand binding , AhR translocates to the nucleus , dimerizes with the AhR nuclear translocator protein ( Arnt ) , and regulates transcription by interaction with dioxin response elements ( DREs ) in target genes , most notably specific cytochrome P 450 ( CYP ) family members . ^^^ AhR and Arnt proteins were present in mouse cerebellum from birth throughout postnatal development . ^^^ AhR and Arnt were also expressed in cerebellar granule neuroblast cultures . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Gestational exposure of Ahr and Arnt hypomorphs to dioxin rescues vascular development . ^^^ Previously , we have demonstrated that the AHR and its heterodimeric partner , the AHR nuclear translocator ( ARNT ) , play a role in the developmental closure of a hepatic vascular shunt known as the ductus venosus ( DV ) . ^^^ To investigate the mechanism of DV closure , we generated hypomorphic alleles of the Ahr and Arnt loci . ^^^ By manipulating gestational exposure , the patent DV in AHR or ARNT hypomorphs could be efficiently closed by dioxin exposure as early as embryonic day 12 . 5 and as late as embryonic day 18 . 5 . ^^^ These findings define the temporal regulation of receptor activation during normal ontogeny and provide evidence to support the idea that receptor activation and AHR ARNT heterodimerization are essential for normal vascular development . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Moreover , down regulation of the aryl hydrocarbon receptor nuclear translocator protein in vivo by adenovirus mediated RNA interference to suppress AhR activity provided wild type mice partial protection from Jo 2 induced lethality . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Hypoxia inducible factor 1alpha ( HIF 1alpha ) and aryl hydrocarbon receptor ( AhR ) both require dimerization with AhR nuclear translocator ( ARNT ) to initiate transcription of their respective target genes . ^^^ It has been proposed that competition for ARNT results in decreased targeting of AhR to cytochrome P 450 1A1 ( CYP1A1 ) under hypoxia . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
CYP1A1 and CYP1A2 mRNA levels in the liver of menadione treated rats were approximately 11 . 8 and 1 . 8 fold higher than in controls , respectively , whereas the expression of the CYP1A regulatory proteins aryl hydrocarbon receptor ( AhR ) and AhR nuclear translocator ( Arnt ) was not changed at the mRNA level . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In the present study , the effect of tumor necrosis factor ( TNF ) alpha and lipopolysaccharides ( LPS ) on the constitutive and inducible expression of the AHR regulated genes cyp1a1 , GST Ya , and QOR was determined in murine hepatoma Hepa 1c1c7 ( WT ) , AHR deficient ( C 12 ) , and AHR nuclear translocator protein ( ARNT ) deficient ( C 4 ) cells . ^^^ These results show that the down regulation of AHR regulated genes by TNF alpha and LPS is dependent on the presence of both heterodimeric transcription factors , AHR and ARNT . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In reporter gene assays , the Cyp2a5 promoter region 3033 to +10 mediated a 2 to 5 fold induction of luciferase activity by TCDD treatment in primary hepatocytes and in Hepa 1 hepatoma cells with an intact AHR / AHR nuclear translocator ( ARNT ) complex . ^^^ In Hepa 1 variant cell lines with deficiencies in the AHR / ARNT complex , the absence of ARNT abolished the induction . ^^^ A putative AHR response element ( XRE ) was identified in the Cyp2a5 promoter at the position 2514 to 2492 and found to interact with the AHR / ARNT heterodimer . ^^^ In conclusion , our studies showed that AHR ligands up regulate Cyp2a5 transcriptionally by an AHR / ARNT dependent mechanism and established Cyp2a5 as a novel AHR regulated gene . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
AOX 1 mRNA levels were not increased by dioxin in mutant derivatives of the Hepa 1 cell line lacking either functional aryl hydrocarbon receptor ( AHR ) or aryl hydrocarbon receptor nuclear translocator ( ARNT ) proteins , thus demonstrating that transcriptional induction of AOX 1 in response to dioxin occurs through the AHR pathway . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
At both 6 and 96 h after a single dose of 50 microg / kg TCDD , significant elevations were found in mRNA levels of AHR repressor ( AHRR ) , CYP1A1 and CYP1A2 , but not those of AHR , ARNT or ARNT 2 . ^^^ Differences between H / W and L E rats appeared in constitutive levels of AHR and ARNT and in TCDD induced levels of CYP1A2 , AHRR , AHR and ARNT , which all were about two to four fold lower in H / W rats . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The amount of CYP1A2 mRNA and regulatory proteins for signal activation of CYP1A1 ( AhR and Arnt ) remained unchanged after treatment with alpha tocopherol . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In both experiments , TCDD caused liver damage and increase in relative liver weights , augmented the EROD activities and CYP1A1 expression , and increased the aryl hydrocarbon receptor ( AhR ) mRNA expression , but did not alter the AhR nuclear translocator ( ARNT ) mRNA expression . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Many PAHs exert their effects by activating a family of basic helix loop helix ( bHLH ) transcription factors , the arylhydrocarbon receptor ( AhR ) and the arylhydrocarbon receptor nuclear translocator ( ARNT ) , which result in the expression of AhR target molecules . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
AhR and its nuclear partner ARNT are expressed in the female reproductive tract in a variety of species and several indications suggest that the AhR might play a pivotal role in the physiology of reproduction . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
For instance , the expression of CYP 1 genes can be induced by AhR , which dimerizes with the AhR nuclear translocator ( Arnt ) , in response to many polycyclic aromatic hydrocarbon ( PAHs ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
ER alpha AHR ARNT protein protein interactions mediate estradiol dependent transrepression of dioxin inducible gene transcription . ^^^ The aryl hydrocarbon receptor ( AHR ) and the aryl hydrocarbon receptor nuclear translocator ( ARNT ) form a heterodimeric transcription factor upon binding a wide variety of environmental pollutants , including 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin ( TCDD ) . ^^^ In this study , we demonstrate that a significant component of TCDD inducible Cyp1a1 transcription is the result of recruitment of estrogen receptor ( ER ) alpha by AHR / ARNT as a transcriptional co repressor . ^^^ Both AHR and ARNT were capable of interacting directly with ER alpha , as ascertained by glutathione S transferase pull down . 17Beta estradiol repressed TCDD activated Cyp1a1 and Cyp1b1 gene transcription in MCF 7 cells in the presence of cycloheximide , as determined by reverse transcription / real time PCR . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Cytochrome P 450 1A1 and 1A2 ( CYP1A1 and CYP1A2 ) , glutathione S transferase M 1 ( GSTM 1 ) , aryl hydrocarbon receptor ( AHR ) , aryl hydrocarbon receptor nuclear translocator ( ARNT ) and catechol O methyltransferase ( COMT ) mRNA levels in the mouse liver were measured by a semi quantitative reverse transcription polymerase chain reaction ( RT PCR ) method . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Dioxin and estrogen linked genes ( AhR , ERalpha , ERbeta , CYP1A1 and ARNT ) expressed were determined with the RT PCR method . ^^^ In the low FBS concentration medium with dioxin , AhR and ERa expression were increased with the proliferation of Caov 3 cells ; CYP1A1 and ARNT were stable . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Interaction of polychlorinated biphenyls ( PCBs ) with the aryl hydrocarbon receptor ( AhR ) / nuclear translocator ( ARNT ) system might interfere with the mechanisms controlling the sexual differentiation of the developing hypothalamus . ^^^ The aim of this study was to evaluate the presence of AhR / ARNT in brain cells and the developmental profile of their expression in the hypothalamus of male and female rats during the perinatal period . ^^^ Brain accumulation of the main PCB congeners after prenatal exposure to Aroclor 1254 and its influence on hypothalamic expression of AhR / ARNT was also assessed . ^^^ The results show that : ( a ) AhR and ARNT are expressed both in neurons and in glia ; ( b ) AhR expression progressively increases in the developing hypothalamus particularly in males , while ARNT is relatively constant in both sexes ; ( c ) the prenatal administration of Aroclor to dams produces a differential accumulation of PCBs , depending on the chlorine atom number , and stimulates AhR expression only in the male hypothalamus . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In this study , mouse cDNA microarrays representing 4858 genes were used to examine differences in basal gene expression between mouse Hepa 1c1c7 wild type and c 1 ( truncated Cyp1a1 protein ) , c 4 ( AhR nuclear translocator , ARNT , deficient ) , and c 12 ( low AhR levels ) mutant cell lines . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Furthermore , AhR ( polycyclic aromatic hydrocarbon receptor ) , when activated by the binding of aromatic hydrocarbons ( Ah ) forms a complex with the aryl hydrocarbon nuclear translocator chaperone protein ( Arnt ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The data presented here demonstrate that normal melanocytes and melanoma cells express the AhR and Arnt and are responsive to activation by TCDD . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor ( AhR ) is a basic helix loop helix DNA binding protein that forms a transcriptionally active heterodimer with the AhR nuclear translocator ( Arnt ) protein . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
PAH exposure activates the aryl hydrocarbon transcription activating factor ( AhR ) , resulting in nuclear translocation , binding to the aryl hydrocarbon nuclear translocator ( ARNT ) , which thereby increases expression of a pool of carcinogen metabolizing enzymes . ^^^ Our results demonstrate that sanguinarine : ( 1 ) results in formation of the AhR ARNT complex , ( 2 ) induces AhR associated gene expression , ( 3 ) inhibits cytochrome P 450 1A1 ( CYP 1A1 ) microsomal oxidative activity and ( 4 ) pretreatment upregulates CYP 1A1 function . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The human hepatoma cell line SK HEP 1 expresses AhR and ARNT . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Analysis of the AhR , ARNT , and AhRR gene polymorphisms : genetic contribution to endometriosis susceptibility and severity . ^^^ OBJECTIVE : To explore whether polymorphisms in AhR , ARNT , and AhRR contribute to endometriosis susceptibility and severity . ^^^ MAIN OUTCOME MEASURE ( S ) : AhR , ARNT , and AhRR polymorphisms were genotyped using real time polymerase chain reaction ( PCR ) analysis . ^^^ Odds ratios and 95 % confidence intervals were calculated for AhR , ARNT , and AhRR genotypes to evaluate the risk of endometriosis . ^^^ No statistically significant association was observed between AhR codon 554 or ARNT codon 189 polymorphisms and endometriosis . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Four plasmid transfections with vectors co expressing the spineless ( Ss ) and tango ( Tgo ) proteins , the Drosophila melanogaster homologues of mammalian AhR and ARNT , have indicated that these proteins enhance basal expression of the CYP6B1 promoter but not the magnitude of its xanthotoxin and benzo [ alpha ] pyrene induction . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In addition , the transactivation domain ( TAD ) appears to determine the presence of XAP 2 in the nuclear ligand bound AhR / hsp90 complex prior to association with the AhR nuclear translocator protein ( ARNT ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Aryl hydrocarbon receptor ( AHR ) and AHR nuclear translocator ( ARNT ) expression in Baikal seal ( Pusa sibirica ) and association with 2 , 3 , 7 , 8 TCDD toxic equivalents and CYP 1 expression levels . ^^^ To understand the regulation mechanism of AHR and AHR nuclear translocator ( ARNT ) expression in wild Baikal seal ( Pusa sibirica ) population contaminated by PHAHs , the present study investigated hepatic mRNA expression levels of AHR and its heterodimer , ARNT genes , in association with biological index ( age , gender and body weight ) , PHAH accumulation and expression levels of cytochrome P 450 ( CYP ) 1A and 1B . ^^^ In contrast to AHR expression , neither the total nor individual congener TEQs influenced ARNT at the transcriptional level . ^^^ ARNT mRNA showed significant negative correlations with CYP1A / 1B protein ( p = 0 . 027 and p = 0 . 006 ) and CYP1A1 mRNA expression levels ( p = 0 . 039 ) , implying the existence of different transcriptional regulation between AHR and ARNT genes and negative regulation by CYP1A / 1B mediated signaling pathways . ^^^ The present findings may render significant insight on the basic mechanisms underlying regulation of AHR and ARNT expressions associated with biological factors and PHAH exposure in wild mammalian populations . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor ( AhR ) / AhR nuclear translocator ( ARNT ) heterodimer complex activates gene transcription by binding to the DREs of CYP1B1 . ^^^ There was no change in the expressions of AhR and ARNT . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Upon transient , co expression of ARNT with AhR A375I or AhR A375F in AhR KO cells , these mutants exhibited significant ligand independent transcriptional activity . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
This generation of ROS by UCB is not dependent on aryl hydrocarbon receptor ( Ahr ) signaling , as cells deficient in the Ahr ( C 12 cells ) or the Ahr nuclear translocator protein ( Arnt ; C 4 cells ) were as efficient at generating ROS as wild type ( WT ) Hepa 1c1c7 cells . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The AhRR shares structural similarities with Aryl hydrocarbon receptor ( AhR ) and AhR nuclear translocator ( ARNT ) . ^^^ The AhRR is thought to be involved in transcriptional control of AhR regulated genes by sequestering ARNT . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The AHR pathway in zebrafish is considerably more complex than in mammals , with at least three zebrafish AHR genes ( zfAHR1a , zfAHR1b , and zfAHR 2 ) and two ARNT genes ( zfARNT 1 and zfARNT 2 ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
TCDD exposure to Hepa 1 cells with reduced aryl hydrocarbon receptor nuclear translocator ( ARNT ) , showed ligand induced degradation of the AHR that was not blocked by AD . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
AhR and ARNT expression was detected both as mRNA and on the protein level . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor ( AHR ) and its DNA binding partner , the aryl hydrocarbon receptor nuclear translocator ( ARNT ) are basic helix loop helix / PAS proteins . ^^^ The goal of the current study was to determine the extent to which residues R 14 and R 15 contained within the basic region of the AHR contribute to the DNA binding affinity and stability of the AHR / ARNT heterodimer . ^^^ Using a two site DNA binding model , we also found that AHR / ARNT heterodimer does not participate in cooperative binding , as binding of the second dimer appears to be prohibited by occupation of the first . ^^^ The present results revealed that DNA binding stability of the AHR / ARNT heterodimer is an important requirement for its transactivation capabilities and that this stability is governed , in part , by residues R 14 and R 15 that lie within the basic region of the AHR . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Finally , quantitative reverse transcription polymerase chain reaction revealed that expression of AhR and Arnt mRNA within enriched hematopoietic precursors oscillates with a circadian period . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In response to aryl hydrocarbon receptor ( AhR ) ligands , cytoplasmic AhR becomes activated and then translocates to the nucleus where it forms a complex with the aryl hydrocarbon receptor nuclear translocator ( ARNT ) . ^^^ Using a standard chromatin immunoprecipitation assay , we have demonstrated that BRCA 1 is recruited to the promoter regions of CYP1A1 and CYP1B1 along with ARNT and / or AhR following xenobiotic exposure . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Identification of the Ah receptor nuclear translocator protein ( Arnt ) as a component of the DNA binding form of the Ah receptor . ^^^ The Ah receptor nuclear translocator protein ( Arnt ) is required for Ah receptor function . ^^^ Arnt is now shown to be a structural component of the XRE binding form of the Ah receptor . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In addition to the BR / HLH domain , the N terminal domain of the Ah receptor has extensive sequence similarity to the human ARNT ( aryl hydrocarbon receptor nuclear translocator ) protein and two regulatory proteins of Drosophila , Sim and Per . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
However , the genetic basis for this polymorphism might be inherited in the genes coding for proteins responsible for the induction of CYP1A1 , ie , the Ah receptor or the ARNT protein . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The sequences of four peptides from samples containing both the 96 and 98 kDa proteins are all highly similar to segments of the human Ah receptor nuclear translocator ( Arnt ) protein . ^^^ These data show that the Ah receptor itself and two forms of the Arnt protein are the major components of the purified DNA binding form of receptor . ^^^ In gel shift assays the purified receptor forms two specifically bound complexes with a xenobiotic responsive element ( XRE ) , which may correspond to Ah receptor heterodimers with either of the two forms of Arnt protein . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In target cells , TCDD initially binds to the aryl hydrocarbon ( Ah ) receptor which accumulates in the nucleus as an Ah receptor : aryl hydrocarbon nuclear translocator ( Arnt ) protein heterodimeric complex . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
That the 98 / 96 kDa protein is the Ah receptor nuclear translocator ( Arnt ) ; 2 . ^^^ That the 110 , the 106 kDa , and the Arnt proteins are members of a multicomponent protein complex . cDNA cloning studies indicate that the 106 kDa protein is the rat Ah receptor . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The results with these mutants suggested that Ah receptor nuclear translocator protein , ARNT , is needed for ALDH 3 expression . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
TCDD first binds with its cytosolic Ah receptor , translocates into the nucleus , dimerizes with `` arnt ' ' ( a nuclear transcription factor ) , and activates genes containing `` xenobiotic responsive element ' ' ( XRE ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The encoded protein ( ARNT ) is required for ligand dependent translocation of the Ah receptor to the nucleus and its binding to the XRE . ^^^ ARNT and the Ah receptor form a heterodimeric complex which binds the XRE in a fashion such that both subunits bind the XRE directly . ^^^ Both ARNT and the Ah receptor contain basic helix loop helix motifs . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
We cloned from mouse hepatoma cells a cDNA which encodes the Ah receptor nuclear translocator ( Arnt ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The results indicated that nuclear translocation was primarily inhibited using cytosol from mutant cells confirming the requirement for the aryl hydrocarbon receptor nuclear translocator protein for formation of the nuclear Ah receptor complex . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The Ah receptor nuclear translocator gene ( ARNT ) is located on q 21 of human chromosome 1 and on mouse chromosome 3 near Cf 3 . ^^^ We have mapped the Ah receptor nuclear translocator ( ARNT ) gene to a conserved linkage group located on mouse chromosome 3 and human chromosome 1 . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The Ah receptor nuclear translocator protein ( ARNT ) is required for binding of the Ah ( dioxin ) receptor to the xenobiotic responsive element ( XRE ) , and is a structural component of the XRE binding form of the Ah receptor . ^^^ Since the vitamin D receptor has similarities to the Ah receptor , we investigated whether ARNT is also required for vitamin D receptor activity . ^^^ Two lines of evidence demonstrate that ARNT is not required for vitamin D receptor activity , and therefore does not correspond to the vitamin D receptor accessory protein : 1 ) Antibodies to ARNT have no effect on binding of the vitamin D receptor to the VDRE . 2 ) c 4 , a mutant of Hepa 1 cells that is defective in ARNT activity , and in which binding of the Ah receptor to the XRE does not occur , possesses a vitamin D receptor with full activity for binding the VDRE . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Recent cloning experiments have demonstrated that the AH receptor is structurally related to the Per , ARNT and Sim proteins . ^^^ Adjacent to the PAS domain in the AH receptor , ARNT and Sim proteins is a basic / helix loop helix ( bHLH ) domain that appears to mediate heterodimerization and sequence specific DNA binding properties . ^^^ The observation that the bHLH domain is present in the AH receptor and the ARNT protein , a factor required for proper AH receptor function , suggests that these proteins are heterodimeric partners that activate gene expression in a manner similar to Myc / Max and MyoD / E2A . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
A factor binding to the xenobiotic responsive element ( XRE ) of P 4501A1 gene consists of at least two helix loop helix proteins , Ah receptor and Arnt . ^^^ Association of the Ah receptor and Arnt ( Ah receptor nuclear translocator ) in an XRE binding complex was examined by anti Ah receptor and Arnt antibodies . ^^^ These results indicate that Ah receptor and Arnt proteins are components of the XRE binding factor and suggest that Arnt as well as the Ah receptor are localized in the cytosol of nontreated cells . ^^^ The Ah receptor present in C 4 cells , a mutant of Hepa 1 cells defective in the Arnt function , showed an inducer dependent association with Arnt synthesized in an in vitro translation system . ^^^ Co transfection of the expression plasmids of the Ah receptor and Arnt exhibited synergistically more activated transcription from a reporter gene pMC6 . 3k consisting of the P 4501A1 gene promoter and enhancer than transfection with either of the two plasmids alone . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Mouse AHD 3 mRNA levels are increased by dioxin in mouse Hepa 1c1c7 hepatoma wild type ( wt ) cells but not in the Ah receptor nuclear translocator ( ARNT ) defective ( c 4 ) mutant line , indicating that the induction process is mediated by the Ah ( aromatic hydrocarbon ) dioxin binding receptor . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Analyses of the induction mechanism using genetic , biochemical , and molecular biological approaches have revealed a novel transcriptional regulatory pathway that involves ligand dependent heterodimerization between two basic helix loop helix proteins ( the Ah receptor and Arnt ) , interaction of the heterodimer with a xenobiotic responsive enhancer , transmission of the induction signal from the enhancer to the CYP1A1 promoter , and alterations in chromatin structure . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Structure and function of PAS proteins : Ah receptor , Arnt , and others ] . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Cloning and selective expression in brain and kidney of ARNT 2 homologous to the Ah receptor nuclear translocator ( ARNT ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
We have isolated and characterized a cDNA for a novel Per Arnt / AhR Sim basic helix loop helix ( bHLH PAS ) factor that interacts with the Ah receptor nuclear translocator ( Arnt ) , and its predicted amino acid sequence exhibits significant similarity to the hypoxia inducible factor 1alpha ( HIF1alpha ) and Drosophila trachealess ( dTrh ) gene product . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Inhibitors of serine / threonine specific protein phosphatases stimulate transcription by the Ah receptor / Arnt dimer by affecting a step subsequent to XRE binding . ^^^ The activity of the Ah receptor / Arnt dimer can be decreased by treatments causing the down regulation of protein kinase C and decreasing the nuclear accumulation of the receptor . ^^^ These results suggest that serine / threonine specific protein phosphatases can act at a level subsequent to XRE binding to inhibit the ability of the Ah receptor / Arnt dimer to stimulate transcription . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The predicted novel factor polypeptide had bHLH and PAS domains that were highly homologous with those of Ah receptor nuclear translocator ( Arnt ) and Arnt 2 . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In contrast , RT PCR analysis of the Ah receptor nuclear translocator ( Arnt ) protein showed that HepG 2 cells expressed the expected 2 . 6 kb transcript , whereas a 1 . 3 kb transcript was the major product in MDA MB 231 cells . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Upon exposure to the above conditions , HIF 1 alpha is upregulated and heterodimerizes with the Ah receptor nuclear translocator ( ARNT , also known as HIF 1 beta ) , the heterodimeric complex binds TACGTG containing genomic enhancer elements , and activates transcription of target genes . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Members of the CYP 1 gene family are induced by polycyclic aromatic hydrocarbons and this process is mediated by the basic helix loop helix proteins : the Ah receptor and its heterodimeric partner Arnt . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
However , the constellation of transcription factors which appear to interact with the AhRE in constitutively expressing corneal cells does not include either the Ah receptor nor the prototypical ARNT protein ( Boesch et al . , 1998 ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Expression of the Ah receptor and Arnt which are involved in the transcriptional activation of the CYP 1 genes was also studied . ^^^ The Ah receptor mRNA and Arnt mRNA were consistently expressed both in kidney tumours and normal kidney . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Ligand treatment induced a 50 fold increase in beta galactosidase activity from a reporter plasmid in yeast engineered to express human Ah receptor and Ah nuclear translocator ( Arnt ) proteins . ^^^ Although yeast have no natural counterpart to the Ah receptor pathway , expression of human Ah receptor and Arnt under the appropriate conditions provides a functional model system for studying Ah receptor activation and signal transduction . . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The basic helix loop helix / Per ARNT Sim homology domain dioxin receptor ( DR ) translocates to the nucleus upon binding of aromatic hydrocarbon ligands typified by dioxin , whereupon it partners the Ah receptor nuclear translocator and initiates transcription . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The Ah receptor nuclear translocator protein ( ARNT ) plays a central role as a common heterodimerization partner . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In addition , although the involvement of the Ah receptor nuclear translocator ( ARNT ) in xenobiotic induction of ALDH 3 is well established , the role of ARNT in constitutive ALDH 3 expression is not clear . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Expressions of aryl hydrocarbon ( Ah ) receptor and Ah receptor nuclear translocator ( ARNT ) , which mediate induction of the CYP 1 family , were also detected in all cell types . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Transcriptional activation of hypoxia regulated genes is mediated by hypoxia inducible factor 1 ( HIF 1 ) , a heterodimer of HIF 1alpha and ARNT ( Ah receptor nuclear translocator ; HIF 1beta ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Gel mobility shift analysis confirmed that TCDD induction of nuclear proteins specifically bound to the UGT1A1 XRE , and competition experiments with Ah receptor and Arnt antibodies demonstrated that the nuclear protein was the Ah receptor . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aim of this study was to clarify these potentially misleading findings , by analysing components of the Ah receptor pathway ( CYP1B1 , CYP1A1 , Ah receptor and ARNT ) using a combination of quantitative real time RT PCR and immunoblotting . ^^^ Three human cell lines ( MOG G CCM , MCF 7 and HEPG 2 ) known to differentially express CYP1A1 and CYP1B1 mRNA and protein were exposed to the Ah receptor agonist 3 MC , and basal and inducible levels of CYP1A1 , CYP1B1 , Ah receptor and ARNT were determined . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Lower incidence of mammary tumors in SPI fed rats was associated with : 1 ) reduced terminal end bud numbers ( p < 0 . 05 ) , 2 ) lower expression of the phase 1 enzyme CYP1B1 ( p < 0 . 05 ) and 3 ) reduced expression of the Ah Receptor and ARNT ( p < 0 . 05 ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In an effort to better understand the Ah receptor nuclear translocator ( Arnt ) dependent signaling mechanisms , we employed a phage display system to identify Arnt interacting peptides . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Most toxic effects induced by TCDD are mediated by binding to the Ah receptor ( Ah R ) which binds together with a second protein , ARNT , to the respone elements of a number of target genes , and thus modulates gene expression . `` Dioxins ' ' are strong promotors , but weak initiators . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor nuclear translocator ( Arnt ) is a basic helix loop helix transcription factor that heterodimerizes with the aryl hydrocarbon receptor to mediate signal transduction pathways inducible by 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin and other planar aromatic hydrocarbons . ^^^ Immunofluorescence analysis of Arnt in three cell lines using two MAbs ( to distinct epitopes ) provides evidence that suggests that the aryl hydrocarbon receptor heterodimerizes with Arnt in the nucleus . . ^^^ Physicochemical and immunocytochemical analysis of the aryl hydrocarbon receptor nuclear translocator : characterization of two monoclonal antibodies to the aryl hydrocarbon receptor nuclear translocator . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The dioxin receptor is a ligand dependent transcription factor that binds to target DNA sequences ( xenobiotic responsive elements , XREs ) following ligand dependent dimerization with its partner factor , Arnt ( aryl hydrocarbon receptor nuclear translocator ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The role of the aryl hydrocarbon receptor nuclear translocator ( ARNT ) in hypoxic induction of gene expression . ^^^ One partner , HIF 1beta , had been recognized previously as the aryl hydrocarbon receptor nuclear translocator ( ARNT ) , an essential component of the xenobiotic response . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The HIF 1alpha and HIF 1beta ( ARNT ; aryl hydrocarbon receptor nuclear translocator ) subunits were coimmunoprecipitated from nuclear extracts , indicating that these proteins heterodimerize in the absence of DNA . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
When reporters containing VEGF sequences , in the context of the native VEGF or heterologous simian virus 40 promoter , were cotransfected with expression vectors encoding HIF 1alpha and HIF 1beta ( ARNT [ aryl hydrocarbon receptor nuclear translocator ] ) , reporter gene transcription was much greater in both hypoxic and nonhypoxic cells than in cells transfected with the reporter alone . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
This protein complex is composed of two subunits , HIF 1alpha and 1beta ( aryl hydrocarbon receptor nuclear translocator , ARNT ) . ^^^ Absolute requirement of aryl hydrocarbon receptor nuclear translocator protein for gene activation by hypoxia . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Isolation and expression of cDNAs from rainbow trout ( Oncorhynchus mykiss ) that encode two novel basic helix loop Helix / PER ARNT SIM ( bHLH / PAS ) proteins with distinct functions in the presence of the aryl hydrocarbon receptor . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
HIF 1 is a heterodimer consisting of an alpha and a beta subunit , both belonging to the basic helix loop helix Per aryl hydrocarbon receptor nuclear translocator Sim ( PAS ) family of transcription factors . ^^^ Whereas HIF 1alpha is a novel member of this family , HIF 1beta is identical to the aryl hydrocarbon receptor nuclear translocator , previously recognized to be involved in xenobiotic metabolism . cDNA cloning revealed that mouse HIF 1alpha can be expressed as two mRNA isoforms containing alternative 5 ' untranslated regions and two different predicted translational start sites . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Because HIF 1beta is also essential for the xenobiotic response ( in which it is termed the aryl hydrocarbon receptor nuclear translocator ) , we also studied c 31 cells , which have a different defect in the xenobiotic response and form the HIF 1 complex normally . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Transient co expression of the two HIF 1 subunits HIF 1alpha and aryl hydrocarbon receptor nuclear translocator ( ARNT ) / HIF 1beta resulted in enhanced reporter gene expression even under normoxic conditions . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
This protein is a member of a rapidly expanding family of gene products possessing basic helix loop helix ( bHLH ) and hydrophobic PAS ( designated a conserved region among PER , ARNT [ aryl hydrocarbon receptor nuclear translocator ] and SIM ) protein association domains . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The HIF 1 complex is composed of two protein subunits : HIF 1beta / ARNT ( aryl hydrocarbon receptor nuclear translocator ) , which is constitutively expressed , and HIF 1alpha , which is not present in normal cells but induced under hypoxic conditions . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Gel mobility shift analysis indicated that the aryl hydrocarbon receptor and its partner Arnt bind to the XRE . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
RT PCR analyses also demonstrated elevated constitutive aryl hydrocarbon receptor nuclear translocator mRNA content ( an approximately two fold increase ) in skin tumors . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The transcriptional response to lowered oxygen levels is mediated by the hypoxia inducible transcription factor ( HIF 1 ) , a heterodimer consisting of the constitutively expressed aryl hydrocarbon receptor nuclear translocator ( ARNT ) and the hypoxic response factor HIF 1alpha . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Hypoxia inducible factor 1 ( HIF 1 ) is a heterodimeric DNA binding complex composed of two basic helix loop helix PAS proteins : HIF 1 beta / ARNT ( aryl hydrocarbon receptor nuclear translocator ) , which is constitutively expressed , and HIF 1 alpha , which is not present in normoxic cells but induced under hypoxic conditions . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In vitro produced zfAhR 2 protein dimerizes with the rainbow trout aryl hydrocarbon receptor nuclear translocator ( rtARNTb ) and binds dioxin response elements derived from the rainbow trout CYP1A gene . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
HIF 1 consists of two subunits , HIF 1alpha and the aryl hydrocarbon receptor nuclear translocator ( ARNT ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Protein kinase C modulates aryl hydrocarbon receptor nuclear translocator protein mediated transactivation potential in a dimer context . ^^^ Protein kinase C ( PKC ) and protein kinase A ( PKA ) mediated modulation of the transactivation potential of human aryl hydrocarbon receptor nuclear translocator ( hARNT ) , a basic helix loop helix ( bHLH ) PAS transcription factor , and the bHLH ZIP transcription factors USF 1 ( for upstream regulatory factor 1 ) and c Myc were examined . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The processes responsible for the limited ability to divide and long survival of neurons are not well understood but may involve aryl hydrocarbon receptor nuclear translocator 2 ( ARNT 2 ) , a recently identified protein , apparently belonging to the basic helix loop helix superfamily of transcription factors , which is expressed almost exclusively in brain during the whole lifetime . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
MCF 10A cells were found by polymerase chain reaction to express cytochrome P4501A and P4501B isozymes as well as the aryl hydrocarbon receptor and aryl hydrocarbon receptor nuclear translocator mRNAs associated with cytochrome P 450 induction . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Analysis of genomic clones extending further upstream of the Ctsk gene identified the 3 ' end of the gene coding for arylhydrocarbon receptor nuclear translocator ( Arnt ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
HIF 1 , which is a heterodimeric complex of HIF 1 alpha and aryl hydrocarbon receptor nuclear translecator ( ARNT ) , binds to HIF 1 element . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
This transactivation was enhanced further by cotransfection of an aryl hydrocarbon receptor nuclear translocator expression plasmid . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
As well as HIF 1alpha , HIF 2alpha forms a heterodimeric complex with the aryl hydrocarbon receptor nuclear translocator and upregulates hypoxia inducible genes such as vascular endothelial growth factor . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
We have cloned a cardiovascular restricted basic helix loop helix factor that interacts with arylhydrocarbon receptor nuclear translocator ( ARNT ) in a yeast two hybrid screen . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Human aryl hydrocarbon receptor nuclear translocator gene ( ARNT ) D / N511 polymorphism . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
To understand the potential underlying molecular mechanisms we studied the expressions of cytochrome P 450 genes ( CYP1A1 , CYP1A2 , and CYP1B1 ) , which are induced by dioxin , and the expressions of cytosolic receptor for dioxin , aryl hydrocarbon receptor , and its nuclear translocator , aryl hydrocarbon receptor nuclear translocator protein , in endometriotic and eutopic endometrial tissues . ^^^ STUDY DESIGN : Levels of transcripts of CYP1A1 , CYP1A2 , CYP1B1 , aryl hydrocarbon receptor , and aryl hydrocarbon receptor nuclear translocator protein were determined by a quantitative reverse transcriptase polymerase chain reaction and Southern blot assay in total ribonucleic acid samples from endometriotic and eutopic endometrial tissues . ^^^ Levels of transcripts of CYP1A1 , CYP1A2 , CYP1B1 , aryl hydrocarbon receptor , and aryl hydrocarbon receptor nuclear translocator protein were determined in endometrial and endometriotic tissues during follicular and luteal phases of the cycle and in cultured endometriotic stromal cells treated with forskolin , phorbol diacetate , medroxyprogesterone acetate , and serum . ^^^ CONCLUSION : We demonstrated for the first time the expression of dioxin related transcription factors aryl hydrocarbon receptor and aryl hydrocarbon receptor nuclear translocator protein and target genes CYP1A1 , CYP1A2 , and CYP1B1 in endometriotic tissues and stromal cells . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
HIF 1 consists of two subunits , HIF 1 alpha and the aryl hydrocarbon receptor nuclear translocator ( ARNT ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The HIF 1 complex is composed of the ubiquitously expressed basic helix loop helix / PAS ( bHLH / PAS ) proteins HIF 1alpha and Arylhydrocarbon Receptor Nuclear Translocator ( ARNT ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Hypoxia inducible factors 1alpha and 2alpha ( HIF 1alpha and HIF 2alpha ) are known to dimerize with the aryl hydrocarbon receptor nuclear translocator in mediating this response . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The localization and function of aryl hydrocarbon receptor nuclear translocator ( ARNT ) in the brain are still unclear . ^^^ Aryl hydrocarbon receptor nuclear translocator is induced by kainic acid in rat hippocampal glial cells . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Expression and subcellular localization of the aryl hydrocarbon receptor nuclear translocator ( ARNT ) protein in mouse and chicken over developmental time . ^^^ The aryl hydrocarbon receptor nuclear translocator ( ARNT ) is a basic helix loop helix / Per ARNT Sim ( bHLH / PAS ) transcription factor that is involved in multiple signaling pathways . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor nuclear translocator ( ARNT ) is a transcriptional regulator that heterodimerizes with Per ARNT Sim ( PAS ) proteins . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The vertebrate basic helix loop helix / Per ARNT Sim ( bHLH / PAS protein ) ARNT ( aryl hydrocarbon receptor nuclear translocator ) plays a crucial role in transcriptional regulation as the common subunit of a number of transcriptionally active complexes . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Brain Muscle Arnt Like protein 2 ( BMAL 2 ; Arnt 4 ) ( aryl hydrocarbon receptor nuclear translocator ) is a recently identified basic Helix Loop Helix Per Arnt Sim ( bHLH PAS ) transcription factor , which contributes to a positive regulation of autoregulatory feedback loop in vertebrate circadian clock systems . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor nuclear translocator ( Arnt ) and hypoxia inducible factor ( HIF ) 1alpha mediate cellular responses to hypoxia . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The basic helix loop helix domain of the aryl hydrocarbon receptor nuclear transporter ( ARNT ) can oligomerize and bind E box DNA specifically . ^^^ The aryl hydrocarbon receptor nuclear transporter ( ARNT ) is a basic helix loop helix ( bHLH ) protein that contains a Per Arnt Sim ( PAS ) domain . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In this paper , we report the cloning of a Xenopus bHLH / PAS factor homologous to the mammalian aryl hydrocarbon receptor nuclear translocator ( Arnt ) or Drosophila Tango gene . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Under hypoxia , HIF 1alpha binds to aryl hydrocarbon receptor nuclear translocator ( ARNT , also called HIF 1beta ) to activate expression of genes important for cell survival . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
However , the aryl hydrocarbon receptor and its nuclear partner , aryl hydrocarbon receptor nuclear translocator mRNA transcription , were unaffected by the EA 4 treatment . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The expressions of hypoxia inducible genes are upregulated by hypoxia inducible factor 1 ( HIF 1 ) , which is a heterodimer of HIF 1alpha and HIF 1beta / ARNT ( aryl hydrocarbon receptor nuclear transporter ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The induction of mouse CYP2S1 mRNA by dioxin represents a primary response and required the aryl hydrocarbon receptor and aryl hydrocarbon receptor nuclear translocator proteins . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
All three mPer promoters contain E boxes and respond to the CLOCK / brain and muscle aryl hydrocarbon receptor nuclear translocator ( ARNT ) like protein 1 ( BMAL 1 ) heterodimer . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
HNF 4 was observed to interact with HIF 1alpha and HIF 1beta ( arylhydrocarbon receptor nuclear translocator , ARNT ) during hypoxia . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The cell types expressed similar levels of CYP 2B6 / 7 , CYP 2E1 , P 450 oxidoreductase , the aryl hydrocarbon receptor nuclear translocator , sulfotransferase 1A1 , sulfotransferase 1A3 , epoxide hydrolase , glutathione S transferase M 3 , glutathione S transferase pi and catalase , superoxide dismutase 1 , glutathione peroxidase 1 and glutathione peroxidase 3 . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
In contrast the activation domains of the aryl hydrocarbon receptor and aryl hydrocarbon receptor nuclear translocator of fish and mammals showed no correlation of activity with biochemical composition or species of origin . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Aryl hydrocarbon receptor nuclear translocator 2 ( ARNT 2 ) : structure , gene mapping , polymorphisms , and candidate evaluation for human orofacial clefts . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Western blot analysis revealed that the amount of the beta subunit of HIF 1 , identical to aryl hydrocarbon receptor nuclear translocator 1 ( ARNT 1 ) , and the homolog ARNT 2 increased in nuclear extracts from SH SY5Y cells exposed to anoxia . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
VicK , an atypical PAS domain containing histidine kinase , can be autophosphorylated in vitro , and VicR functions in vitro as a phospho acceptor protein . ( PAS is an acronym formed from the names of the proteins in which the domains were first recognized : the Drosophila period clock protein [ PER ] , vertebrate aryl hydrocarbon receptor nuclear translocator [ ARNT ] , and Drosophila single minded protein [ SIM ] . ) PAS domains are commonly involved in sensing intracellular signals such as redox potential , which suggests that the signal for vic might also originate in the cytoplasm . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Hypoxia induced gene expression depends primarily on stabilization of the alpha subunit of hypoxia inducible factor 1 ( HIF 1alpha ) , which acts as a heterodimeric trans activator with the nuclear protein known as the aryl hydrocarbon receptor nuclear translocator ( Arnt ) . ^^^ Reduction of hypoxia induced transcription through the repression of hypoxia inducible factor 1alpha / aryl hydrocarbon receptor nuclear translocator DNA binding by the 90 kDa heat shock protein inhibitor radicicol . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The beta subunit ( also termed ARNT , aryl hydrocarbon receptor nuclear translocator ) is abundantly expressed in an oxygen independent manner . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
This transcription factor is composed of two subunits : HIF 1alpha and ARNT ( aryl hydrocarbon receptor nuclear translocator ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The basic helix loop helix ( bHLH PAS ) proteins aryl hydrocarbon receptor nuclear translocator ( Arnt ) and Arnt 2 are transcriptional regulators that function as dimerizing partners for several bHLH PAS proteins and also some nonrelated partners . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The correlation of HIF 1alpha and P gp expression was validated by the use of hepatoma tumor spheroids that were either wild type ( Hepa 1 ) or mutant ( Hepa1C4 ) for aryl hydrocarbon receptor nuclear translocator ( ARNT ) , i . e . , HIF 1beta . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The temporal pattern of hypoxic ERO 1 Lalpha induction is very similar to that of genes triggered by the hypoxia inducible transcription factor ( HIF 1 ) and is characteristically mimicked by cobalt and by deferoxamine , but is absent in cells with a defective aryl hydrocarbon receptor translocator ( ARNT , HIF 1beta ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Here we show that ARNT ( aryl hydrocarbon receptor nuclear translocator ) , the obligatory heterodimerization partner for the aryl hydrocarbon receptor and hypoxia inducible factor 1alpha , functions as a potent coactivator of ERalpha and ERbeta dependent transcription . ^^^ These observations , together with the previous role of ARNT as an obligatory partner protein for conditionally regulated basic helix loop helix PAS proteins like the aryl hydrocarbon receptor or hypoxia inducible factor 1alpha , expand the cellular functions of ARNT to include regulation of ERalpha and ERbeta transcriptional activity . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Estrogen increases messenger RNA and immunoreactivity of aryl hydrocarbon receptor nuclear translocator 2 in the rat mediobasal hypothalamus . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Hepa 1 produces functional aryl hydrocarbon receptor nuclear translocator ( ARNT ) . c 4 cells are derived from Hepa 1 , but are ARNT deficient . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Aryl hydrocarbon receptor nuclear translocator ( ARNT ) , a transcription factor of the Per / AHR / ARNT / Sim family , regulates gene expression in response to environmental stimuli including xenobiotics and hypoxia . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Two closely related forms of each of the hypoxia inducible factors alpha and single minded proteins and the general partner protein , aryl hydrocarbon receptor nuclear translocator ( ARNT ) , are present in many cell types . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Transcriptional responses to hypoxia are primarily mediated by hypoxia inducible factor ( HIF ) , a heterodimer of HIF alpha and the aryl hydrocarbon receptor nuclear translocator subunits . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
HIF is a heterodimeric protein complex composed of two members of the basic helix loop helix Per ARNT Sim ( PAS ) ( ARNT , aryl hydrocarbon receptor nuclear translocator ) domain family of transcriptional activators , HIFalpha and ARNT . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
HIF 1 is a heterodimer consisting of an oxygen sensitive HIF 1 and an oxygen independent subunit HIF 1beta ( also known as the aryl hydrocarbon receptor nuclear translocator ARNT ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Transcriptional adaptations to hypoxia are mediated by hypoxia inducible factor ( HIF ) 1 , a heterodimer of HIF alpha and aryl hydrocarbon receptor nuclear translocator subunits . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The temporal pattern of hypoxic TPI induction is very similar to that of genes triggered by the hypoxia inducible transcription factor ( HIF ) and is mimicked characteristically by cobalt and by deferoxamine , but is absent in cells with a defective aryl hydrocarbon receptor nuclear translocator ( ARNT , here HIF 1beta ) and in cells lacking HIF 1alpha protein . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The functional transcription factor exists as a heterodimeric complex consisting of HIF 1alpha and the aryl hydrocarbon receptor nuclear translocator ( ARNT ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Expression of aryl hydrocarbon receptor nuclear translocator ( ARNT ) isoforms in juvenile and adult rainbow trout tissues . ^^^ Whether and where rainbow trout aryl hydrocarbon receptor nuclear translocator ( rtARNT ) isoforms are expressed in juvenile and adult tissues of rainbow trout is unknown . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor nuclear translocator ( ARNT ) is a member of the Per ARNT Sim ( PAS ) protein superfamily , transcription factors that mediate the cellular responses to various developmental signals and environmental conditions . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The expression level of mRNAs of induction mediating proteins aryl hydrocarbon receptor and aryl hydrocarbon receptor nuclear translocator was the same in both cell cultures tested . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Reporter analysis using human Flt 1 promoter and gel mobility shift assays showed that the heterodimer of EPAS 1 and aryl hydrocarbon receptor nuclear translocator binds directly to HIF 1 binding site upstream of Flt 1 promoter and transactivates it . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Aryl hydrocarbon receptor nuclear translocator ( ARNT ) belongs to the basic helix loop helix Per Arnt Sim ( bHLH PAS ) protein which dimerizes with other PAS proteins . ^^^ Although it has a transactivation domain ( TAD ) , ARNT functions as an assistant partner of main factors , such as aryl hydrocarbon receptor and hypoxia inducible factors , rather than acting as a straightforward transcription factor . ^^^ Non hypoxic transcriptional activation of the aryl hydrocarbon receptor nuclear translocator in concert with a novel hypoxia inducible factor 1alpha isoform . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Gene induction by hypoxia involves a dimer of the hypoxia inducible factor ( HIF ) 1 alpha and the nuclear cofactor HIF 1 beta , also called the aryl hydrocarbon receptor nuclear translocator ( ARNT ) , which is also involved in induction of genes in response to aryl hydrocarbon exposure . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
HIF is a heterodimeric transcription factor consisting of alpha and beta aryl hydrocarbon receptor nuclear translocator or ARNT ) subunits . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Inactivation of the arylhydrocarbon receptor nuclear translocator ( Arnt ) suppresses von Hippel Lindau disease associated vascular tumors in mice . ^^^ In order to examine the role of HIF in von Hippel Lindau ( VHL ) associated vascular tumorigenesis , we utilized Cre loxP mediated recombination to inactivate hypoxia inducible factor 1alpha ( Hif 1alpha ) and arylhydrocarbon receptor nuclear translocator ( Arnt ) genes in a VHL mouse model of cavernous liver hemangiomas and polycythemia . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
HIF is a heterodimer consisting of the O 2 regulated subunit , HIF 1alpha , and the constitutively expressed aryl hydrocarbon receptor nuclear translocator , HIF 1beta . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Hypoxia inducible factor ( HIF ) is a heterodimeric transcription factor composed of HIFalpha and the arylhydrocarbon receptor nuclear translocator ( ARNT / HIF1beta ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
We assayed the capacity of extracts of these fruits and vegetables to induce NQO 1 [ by more than 1 . 5 fold : ratio of induction ( cells treated / control ) > 1 . 5 , 8 mg / ml dose ] in two murine hepatoma cell lines : Hepa 1c1c7 and BPrC 1 , a modified cell line that possesses a nonfunctional aryl hydrocarbon receptor nuclear translocator system and is thus nonresponsive to bifunctional inducers . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Whereas TMS down regulated TCDD induced CYP1B1 gene expression , the levels of aryl hydrocarbon receptor and aryl hydrocarbon receptor nuclear translocator mRNA expression were not changed by TMS treatment . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The basic helix loop helix / PER ARNT SIM homology ( bHLH / PAS ) transcription factor ARNT ( aryl hydrocarbon receptor nuclear translocator ) is a key component of various pathways which induce the transcription of cytochrome P 450 and hypoxia response genes . ^^^ Phosphorylation inhibits DNA binding of alternatively spliced aryl hydrocarbon receptor nuclear translocator . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor nuclear translocator ( ARNT ) is a promiscuous bHLH PAS ( Per ARNT Sim ) protein that forms heterodimeric transcriptional regulator complexes with several other bHLH PAS subunits to control a variety of biological pathways , some of which are centrally involved in disease initiation and / or progression . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
OBJECTIVE : The human aryl hydrocarbon receptor nuclear translocator gene ( ARNT ) is crucial for embryonic development . ^^^ The aryl hydrocarbon receptor nuclear translocator gene polymorphism in patients with recurrent miscarriage . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
PAS is an acronym formed from the names of the Drosophila period clock protein ( PER ) , vertebrate aryl hydrocarbon receptor nuclear translocator ( ARNT ) , and Drosophila single minded protein ( SIM ) . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Inactivation of the HIF alpha binding partner , arylhydrocarbon receptor nuclear translocator ( Arnt ) , but not Hif 1alpha , suppressed the development of renal cysts . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
Tissue specific expression of a bHLH PAS protein homologous to ARNT during the development of crustacean Daphnia magna . cDNAs encoding a Daphnia magna homolog of aryl hydrocarbon receptor nuclear translocator ( ARNT ) were isolated and the structural and functional features as well as the expression pattern of their product , DmagARNT , were analyzed . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
The aryl hydrocarbon receptor nuclear translocator ( Arnt ) , also called Hif 1beta , serves as an obligate heterodimerization partner of Hif 1alpha and Hif 2alpha . ^^^
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
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Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
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Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
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Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
NA
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
NA
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
NA
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
NA
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
NA
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
NA
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
NA
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
NA
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
NA
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
NA
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
NA
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
NA
Interacting proteins: P35869 and P27540	Pubmed	SVM Score :0.0
NA