| Interacting proteins: Q16665 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q16665 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q16665 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q16665 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16665 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q16665 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16665 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16665 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16665 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16665 and P27361 |
Pubmed |
SVM Score :0.0 |
| We hypothesize that morphine inhibits myocardial VEGF expression by inhibiting hypoxia induced factor 1alpha ( HIF 1alpha ) and the signal transduction mechanisms involving Erk 1 , 2 MAP kinase ( p42 / p44 ) , and PI 3 kinase activity ( phospho Akt ) . ^^^ RESULTS : Using rat cardiac myocytes in vitro , we show that morphine : ( 1 ) decreases hypoxia induced VEGF ( 121 ) and VEGF ( 165 ) mRNA expression and VEGF protein concentration through an opioid receptor mechanism ; ( 2 ) decreases HIF 1alpha protein expression ( immunoblot ) and nuclear protein binding to the VEGF HIF 1alpha DNA response element ( EMSA ) ; and ( 3 ) inhibits phospho Erk 1 , 2 MAP kinase ( immunoblot ) and phospho Akt kinase activity ( immunoblot ) . ^^^ Using a rat coronary ligation model , we show that morphine treatment : ( 1 ) decreases myocardial VEGF protein expression ( immunohistochemistry ) ; ( 2 ) decreases HIF 1alpha protein expression ( immunoblot ) ; and ( 3 ) decreases phospho Erk 1 , 2 and phospho Akt expression . ^^^ CONCLUSIONS : ( 1 ) Morphine inhibits hypoxia induced VEGF transcription , in part , through an HIF 1alpha mediated mechanism and ( 2 ) morphine inhibition of hypoxia induced HIF 1alpha may be mediated by inhibition of ERK 1 , 2 MAP kinase activity and PI 3 kinase activity . . ^^^ |
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| Interacting proteins: Q16665 and P27361 |
Pubmed |
SVM Score :0.0 |
| PUFAs reduce VEGF expression in human colon cancer cells modulating the COX 2 / PGE2 induced ERK 1 and 2 and HIF 1alpha induction pathway . n 3 Polyunsaturated fatty acids ( PUFAs ) inhibit the development of microvessels in mammary tumors growing in mice . ^^^ Moreover , they inhibited ERK 1 and 2 phosphorylation and HIF 1alpha protein over expression , critical steps in the PGE ( 2 ) induced signaling pathway leading to the augmented expression of VEGF in colon cancer cells . ^^^ |
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| Interacting proteins: Q16665 and P27361 |
Pubmed |
SVM Score :0.0 |
| In contrast the JNK inhibitor SP 600125 and the ERK 1 , 2 inhibitor UO 126 resulted in increase of HIF 1alpha and P glycoprotein in the control as well as the hyperthermia treated samples , indicating negative regulation of intrinsic HIF 1alpha and P glycoprotein expression by ERK 1 , 2 and JNK signaling cascades . ^^^ In summary our data demonstrate that hyperthermia induced upregulation of P glycoprotein and HIF 1alpha is mediated by activation of p 38 , whereas ERK 1 , 2 and JNK are involved in repression of P glycoprotein and HIF 1alpha under control conditions . . ^^^ |
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