| Interacting proteins: Q00613 and P27361 |
Pubmed |
SVM Score :0.64072256 |
| We have found that the two pathways interact directly and that heat shock causes the physical association of ERK 1 with HSF 1 , an interaction that promotes the kinase activity of ERK in heat shocked cells . 0.64072256^^^ |
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| Interacting proteins: Q00613 and P27361 |
Pubmed |
SVM Score :0.0 |
| We previously showed that ERK 1 phosphorylates HSF 1 on serine 307 and leads to secondary phosphorylation by glycogen synthase kinase 3 ( GSK 3 ) on serine 303 within the regulatory domain and that these phosphorylation events repress HSF 1 . ^^^ |
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| Interacting proteins: Q00613 and P27361 |
Pubmed |
SVM Score :0.0 |
| Increases in ERK 1 activity through treatment of cells with sodium vanadate ( SV ) , an inhibitor of the dual specificity MAP kinase phosphatase 1 ( PAC 1 ) , resulted in increased phosphorylation of the heat shock transcription factor 1 ( HSF 1 ) in unheated cells , delayed the activation of HSF 1 by heat shock , and inhibited the induction of hsp 70 by heat shock . ^^^ |
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| Interacting proteins: Q00613 and P27361 |
Pubmed |
SVM Score :0.0 |
| Consistent with this conclusion was the observation that dexamethasone had no effect on activity of the MAPKs ( ERK 1 , ERK 2 , or c jun N terminal kinase ) , which are known to negatively regulate heat shock transcription factor 1 . ^^^ |
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| Interacting proteins: Q00613 and P27361 |
Pubmed |
SVM Score :0.0 |
| Our experiments show that mitogen activated protein kinases ( MAPKs ) of the ERK 1 family phosphorylate HSF 1 on serine residues and repress the transcriptional activation of the heat shock protein 70B ( HSP70B ) promoter by HSF 1 in vivo . ^^^ |
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| Interacting proteins: Q00613 and P27361 |
Pubmed |
SVM Score :0.0 |
| We recently presented evidence that HSF 1 can be phosphorylated by the mitogen activated protein kinase , ERK 1 , and that such phosphorylation appears to negatively regulate the activity of HSF 1 . ^^^ In this report , we have tested the ability of ERK 1 to phosphorylate various HSF 1 deletion mutants . ^^^ Our results show that ERK 1 phosphorylation is dependent on a region of HSF 1 extending from amino acids 280 to 308 . ^^^ The possibility of protein kinases other than ERK 1 phosphorylating HSF 1 was also examined using in gel kinase assays . ^^^ |
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