| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| By contrast , the MAP kinases ERK 1 and ERK 2 were fully activated by TPA or TCR stimulation and were not affected by Ca2+ , CD 28 , or CsA . ^^^ |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| In these cells ERK 1 activation is induced by two distinct stimuli , insulin and tumor promoting agent ( TPA ) . ^^^ While insulin was found to be more potent than TPA for ERK 1 activation , both stimuli produced the same transient activation pattern with a rapid peak ( reached within 5 min ) followed by a fast decrease within 20 min . ^^^ Interestingly , this inhibitor was found to be regulated by insulin and TPA with a profile that is the mirror image of ERK 1 activity . ^^^ Interestingly , phosphatase 2A treatment of extracts from 5 min TPA treated cells ( where the ERK 1 inhibitor was weak ) was able to induce an increase in the ERK 1 repressing activity . ^^^ |
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| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| However , the expression of these proteins was normal and p44mapk activity remained responsive to the tumour promoter TPA . ^^^ |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| In contrast to EGF , the phorbol ester protein kinase C activator 12 O tetradecanoylphorbol 13 acetate ( TPA ) induced a sustained activation of ERK 1 and ERK 2 for at least 2 h . ^^^ |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| GnRH a and TPA apparently activated mainly the MAPK isoform ERK 1 , as revealed by Mono Q fast protein liquid chromatography followed by Western blotting as well as by gel kinase assay . ^^^ |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| These results also indicate that ERK 1 and ERK 2 are regulated by distinct mechanisms during TPA induced HL 60 differentiation , suggesting that their biological roles are nonredundant . . ^^^ |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| SP peptide , epidermal growth factor , and the PKC activator 12 O tetradecanoylphorbol 13 acetate ( TPA ) induced the tyrosine phosphorylation of the Erk 1 and Erk 2 MAP kinases in a concentration dependent manner in U 373 MG cells . ^^^ Pretreatment of the cells with PKC inhibitors , CGP 41251 or tamoxifen , inhibited tyrosine phosphorylation of Erk 1 and Erk 2 MAP kinases induced by low concentrations of SP or TPA and significantly attenuated phosphorylation at high concentrations of SP or TPA . ^^^ Prolonged treatment with TPA resulted in down regulation of PKC and selective inhibition of TPA and SP induced Erk 1 and Erk 2 tyrosine phosphorylation in U 373 MG cells . ^^^ In contrast to CGP 41251 and tamoxifen , G 6976 , a highly selective inhibitor for PKC alpha and PKC beta 1 isozymes , did not inhibit SP or TPA induced tyrosine phosphorylation of Erk 1 and Erk 2 MAP kinases ; rather , it inhibited a signaling pathway leading to the phosphorylation of cAMP responsive element binding protein in U 373 MG cells . ^^^ |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| Nonphosphorylated forms and TPA or EGF induced phosphorylated forms of Erks ( Erk 1 and Erk 2 ) in P cells were much lower than those in P+ cells . ^^^ |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| In contrast , wild type Raf 1 , ERK 1 , ERK 2 , MEK 4 , or JNK 1 produced no change in activity and the dominant negative forms of these kinases failed to suppress TPA dependent transcription . ^^^ |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| TPA induced activation of the SRE was partially inhibited by dominant negative c Raf , ERK 1 , or ERK 2 , and constitutively active mutants of PKC alpha and PKC epsilon activated the transactivation domain of Elk 1 . ^^^ |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| Upon TPA treatment , the activity of ERK 1 and ERK 2 rapidly increased , with maximal induction between 1 and 3 h , while ERK 2 protein levels remained constant . ^^^ |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| The analysis of two distinct mitogen activated protein kinase pathways shows that stress activated protein kinase Jun N terminal kinase activation , resulting in the phosphorylation of ATF 2 , c Jun , and JunD , is required not only for the IL 1 but also for the TPA dependent induction , while the extracellular signal related kinase 1 ( ERK 1 ) and ERK 2 activation is involved in the TPA but not in the IL 1 dependent stimulation of the uPA enhancer . . ^^^ |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| We show that the activities of both ERK 1 and ERK 2 are upregulated in this model in response to TPA . ^^^ |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| Down regulation of PKC and bisindolylmaleimide 1 inhibited both TPA and PDGF BB stimulated Erk 1 activity . ^^^ |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| The TPA induced ERK activity in AL mice was accompanied by increased phosphorylation of ERK 1 and ERK 2 ( P < 0 . 05 ) , which was abrogated in DER mice . ^^^ |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| TPA treatment ( 10 ng / ml for 30 min ) of WB F 344 rat liver epithelial cells strongly activated p 42 and p 44 ERK 1 and 2 , blocked gap junction mediated fluorescent dye coupling , and induced connexin 43 hyperphosphorylation and gap junction internalization . ^^^ |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| MAPK 1 had no effect on constitutive MMP expression but reduced or abolished the TPA up regulation of MMP 9 in MDA MB 231 and MCF 7 . ^^^ |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| Indeed , we found that inhibition of ERK 1 / 2 phosphorylation ( activation ) inhibited TPA induced transglutaminase activity . ^^^ However , inhibition of PLD mediated signal generation had only a small effect on TPA elicited ERK 1 / 2 phosphorylation ( activation ) , whereas inhibition of ERK 1 / 2 did not affect PLD activation , suggesting that these two pathways likely operate largely in parallel . ^^^ |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| Lingonberry extract also prevented TPA induced phosphorylation of ERK 1 , ERK 2 , and MEK1 / 2 . ^^^ |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| Furthermore , tPA induced rapid tyrosine phosphorylation on the beta subunit of LRP 1 , which was followed by the activation of Mek 1 and its downstream Erk 1 and 2 . ^^^ Blockade of Erk 1 / 2 activation by the Mek 1 inhibitor abolished MMP 9 induction by tPA in NRK 49F cells . ^^^ |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| Both TPA and UVR treatment of wild type mice ( a ) increased the expression of proliferating cell nuclear antigen ( PCNA ) and apoptosis ; ( b ) stimulated the expression of cytokines tumor necrosis factor alpha ( TNF alpha ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and granulocyte CSF ( G CSF ) ; and ( c ) increased cyclooxygenase 2 ( COX 2 ) expression and expression of phosphorylated Akt ( p Akt ) , p 38 , extracellular signal regulated kinase 1 ( ERK 1 ) , and ERK 2 . ^^^ |
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| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| ERK 1 ( / ) mice are resistant to development of skin papillomas induced by 7 , 12 dimethylbenz ( a ) anthracene ( DMBA ) and promoted by TPA . ^^^ |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|