| Interacting proteins: Q99626 and P26367 |
Pubmed |
SVM Score :0.0 |
| Glucagon gene transcription activation mediated by synergistic interactions of pax 6 and cdx 2 with the p 300 co activator . ^^^ The homeodomain transcription factors brain 4 , pax 6 , and cdx 2 have been shown to bind to these sites and to transactivate glucagon gene expression . ^^^ In the present study , we investigated the interaction of cdx 2 and pax 6 with p 300 , a co activator coupled to the basal transcription machinery . ^^^ In transient transfection expression experiments , we found that the transactivating effects of cdx 2 and pax 6 on the glucagon gene were greatly enhanced by the additional expression of p 300 . ^^^ This enhancement was due to direct protein protein interactions of both pax 6 and cdx 2 with the N terminal C / H1 domain of p 300 . pax 6 and cdx 2 also directly interacted with one another at the protein level . pax 6 , bound to its DNA recognition site in the glucagon G 1 promoter element , tethered cdx 2 to the molecular complex of pax 6 and p 300 . ^^^ |
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| Interacting proteins: Q99626 and P26367 |
Pubmed |
SVM Score :0.0 |
| Chromatin immunoprecipitation assays demonstrated that Maf proteins associate with insulin and glucagon promoters in beta and alpha cell lines , respectively . c Maf protein stimulated glucagon promoter activity in a transient luciferase assay , and activation of the glucagon promoter by c Maf was more efficient than by the other alpha cell enriched transcription factors , Cdx 2 , Pax 6 , and Isl 1 . ^^^ |
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| Interacting proteins: Q99626 and P26367 |
Pubmed |
SVM Score :0.0 |
| NA |
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