| Interacting proteins: P19525 and P25963 |
Pubmed |
SVM Score :0.0 |
| In vitro studies using purified reagents demonstrate that PKR is capable of phosphorylating 1 kappa B alpha ( the inhibitory subunit of NF kappa B ) in a dsRNA dependent manner . ^^^ This suggests that phosphorylation of 1 kappa B alpha by PKR could be an initial step in the activation of NF kappa B by dsRNA . ^^^ |
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| Interacting proteins: P19525 and P25963 |
Pubmed |
SVM Score :0.0 |
| We present direct evidence that PKR can phosphorylate 1 kappa B alpha ( MAD 3 ) and activate NF kappa B DNA binding activity in vitro . ^^^ |
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| Interacting proteins: P19525 and P25963 |
Pubmed |
SVM Score :0.0 |
| To examine the molecular basis of constitutive NF kappaB DNA binding activity in HIV 1 infected cells , we analyzed the phosphorylation and turnover of IkappaBalpha protein , the activity of the double stranded RNA dependent protein kinase ( PKR ) and the intracellular levels of NF kappaB subunits in the PLB 985 and U 937 myeloid cell models . ^^^ We suggest that HIV 1 infection of myeloid cells induces IFN production and PKR activity , which in turn contribute to enhanced IkappaBalpha phosphorylation and subsequent degradation . ^^^ |
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| Interacting proteins: P19525 and P25963 |
Pubmed |
SVM Score :0.0 |
| The translation initiation factor eIF 2alpha ( alpha subunit of eukaryotic translation initiation factor 2 ) and IkappaBalpha , the inhibitor of the transcription factor NF kappaB , have been proposed as downstream mediators of PKR effects . ^^^ To evaluate the involvement of NF kappaB and eIF 2alpha in the induction of apoptosis by PKR , we have used vaccinia virus ( 10 ) recombinants that inducibly express PKR concomitantly with a dominant negative mutant of eIF 2alpha or a repressor form of IkappaBalpha . ^^^ Coexpression of PKR with a repressor form of IkappaBalpha ( Ser 32 , 36 Ala ) also leads to the inhibition of apoptosis by abolishing NF kappaB induction , while translation remains blocked . ^^^ Treating cells with two different proteasome inhibitors which block IkappaBalpha degradation , prevented PKR induced apoptosis , supporting results from coexpression studies . ^^^ |
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| Interacting proteins: P19525 and P25963 |
Pubmed |
SVM Score :0.0 |
| Here we show that in response to poly ( rI ) . poly ( rC ) ( pIC ) , PKR activates IkappaB kinase ( IKK ) , leading to the degradation of the inhibitors IkappaBalpha and IkappaBbeta and the concomitant release of NF kappaB . ^^^ |
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| Interacting proteins: P19525 and P25963 |
Pubmed |
SVM Score :0.0 |
| As described for other stimuli , following pIC treatment , PKR phosphorylates the NF kappa B inhibitor 1 kappa B alpha at serine 32 before degradation . ^^^ |
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| Interacting proteins: P19525 and P25963 |
Pubmed |
SVM Score :0.0 |
| Activation of the 1 kappa B alpha kinase ( IKK ) complex by double stranded RNA binding defective and catalytic inactive mutants of the interferon inducible protein kinase PKR . ^^^ For example , PKR induces 1 kappa B alpha kinase ( IKK ) activity and 1 kappa B alpha phosphorylation leading to the induction of NF kappa B mediated gene transcription . ^^^ These data strongly support an indirect role of PKR in 1 kappa B alpha phosphorylation by modulating IKK activity through pathways that do not utilize the enzymatic and dsRNA binding properties of PKR . . ^^^ |
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| Interacting proteins: P19525 and P25963 |
Pubmed |
SVM Score :0.0 |
| We found that L RNA activates PKR and induces NF kappa B activation through degradation of its inhibitor 1 kappa B alpha . ^^^ |
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| Interacting proteins: P19525 and P25963 |
Pubmed |
SVM Score :0.0 |
| Here we report that this mutant does not activate PKR , has no effect on the accumulation of IkappaBalpha , and does not cause the translocation of NF kappaB in infected cells . ( 3 ) One hypothesis advanced for the activation of NF kappaB is that it blocks apoptosis induced by viral gene products . ^^^ |
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| Interacting proteins: P19525 and P25963 |
Pubmed |
SVM Score :0.0 |
| Okadaic acid induces tyrosine phosphorylation of IkappaBalpha that mediated by PKR pathway in human osteoblastic MG 63 cells . ^^^ We investigated the functional relationship between PKR and IkappaBalpha phosphorylation by constructing MG 63 PKR K / R cells that produced a catalytically inactive mutant PKR . ^^^ Although IkappaBalpha was degraded phosphorylation of eIF 2 alpha , a substrate of PKR , did not occur in the mutant cells treated with okadaic acid . ^^^ Our results suggest that okadaic acid induced tyrosine phosphorylation of IkappaBalpha was mediated by PKR kinase activity , thus indicating the involvement of this kinase in the control mechanism governing the activation of NF kappaB . . ^^^ |
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| Interacting proteins: P19525 and P25963 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P19525 and P25963 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P19525 and P25963 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19525 and P25963 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19525 and P25963 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19525 and P25963 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19525 and P25963 |
Pubmed |
SVM Score :0.0 |
| NA |
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