Using a nitrocellulose overlay assay with 125I CacyBP / SIP and CacyBP / SIP affinity chromatography , we found that this protein binds purified S100A1 , S100A6 , S100A12 , S100B , and S100P but not S100A4 , calbindin D ( 9k ) , parvalbumin , and calmodulin . ^^^ |