| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.65684523 |
| LMO 2 has been shown to cause tumours when aberrantly expressed and to be able to heterodimerise with TAL 1 to facilitate tumour development . . 0.65684523^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.62826717 |
| We now show that in erythroid cells Lmo 2 forms a novel DNA binding complex , with GATA 1 , TAL 1 and E2A , and the recently identified LIM binding protein Ldb1 / NLI . 0.62826717^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| These oncogenes ( Ttg 1 , Ttg 2 , SCL , LylI , H0X11 ) all belong to classic families of transcription factors , possessing LIM domains , helix loop helix motifs , or homeodomains . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| Defects of the mismatch repair gene MSH 2 are implicated in the development of murine and human lymphoblastic lymphomas and are associated with the aberrant expression of rhombotin 2 ( Lmo 2 ) and Tal 1 ( SCL ) . ^^^ Evaluation of the expression of three T cell malignancy associated genes showed that Rhombotin 2 ( RBTN 2 also known as Lmo 2 ) , TAL 1 ( also known as SCL ) , and HOX 11 were expressed in 100 % , 40 % , and 0 % of the murine tumors , respectively . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| Likewise , the level of expression of the erythroid transcription factors GATA 1 , GATA 2 , NF E 2 , SCL and RBTN 2 , all expressed in untreated K 562 cells , did not increase during sodium phenylacetate induced erythroid differentiation . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| In murine erythroleukemia cells , the endogenous Lbd 1 and LMO 2 proteins exist in a stable complex , whose binding affinity appears greater than that between LMO 2 and the bHLH transcription factor SCL . ^^^ However , Ldb 1 , LMO 2 , and SCL / E12 can assemble as a multiprotein complex on a consensus SCL binding site . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| In collaboration with LMO 1 or LMO 2 , the thymic expression of SCL / tal leads to T ALL at a young age with a high degree of penetrance in transgenic mice . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| Cooperation between the stem cell leukemia ( SCL ) transcription factor and its nuclear partners LMO 1 or LMO 2 induces aggressive T cell acute lymphoblastic leukemia when inappropriately expressed in T cells . ^^^ Maturation to the pre T cell stage is associated with a down regulation of SCL and LMO 1 and LMO 2 , and a concomitant up regulation of E2A and HEB expression . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| Primitive erythropoiesis in the Xenopus embryo : the synergistic role of LMO 2 , SCL and GATA binding proteins . ^^^ SCL and GATA 1 interact in a transcriptional complex with the LIM domain protein LMO 2 . ^^^ We have cloned the Xenopus homolog of LMO 2 and show that it is expressed in a similar pattern to SCL during development . ^^^ SCL and LMO 2 act synergistically to expand the blood island when overexpressed in whole embryos . ^^^ Furthermore , co expression of GATA 1 with SCL and LMO 2 leads to embryos that are ventralized and have blood throughout the dorsal ventral axis . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| Molecular markers have been characterised for various myeloid cell types and their precursor cells , for example : stem cells ( scl , hhex , lmo 2 ) , myeloid lineage precursors ( spi1 / pu . 1 , c / ebp1 ) , heterophil granulocytes ( mpx / mpo ) , macrophages ( L plastin , fms ) . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| This complex , containing hematopoietic specific ( SCL , Lim only 2 ( LMO 2 ) , GATA 1 / GATA 2 ) and ubiquitous ( E2A , LIM domain binding protein 1 [ Ldb 1 ] ) factors , is tethered to DNA via a specificity protein 1 ( Sp 1 ) motif , through direct interactions between elements of the SCL complex and the Sp 1 zinc finger protein . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| The study includes transcription factors which are known to participate in multimeric complexes : GATA 1 , 2 , SCL / tal 1 and Lmo 2 ( whose avian orthologue we have cloned ) , VEGF R 2 , a critical regulator of hemopoietic and endothelial commitment , and hemoglobin used as a marker of the last step in erythroid differentiation . ^^^ Several findings were unexpected . ( 1 ) Two distinct patterns were revealed for GATA 2 , first : low expression , ubiquitous in all mesodermal cells , as soon as cells ingress through the primitive streak ; secondly : high , blood island specific expression . ( 2 ) VEGF R 2 is coexpressed with GATA 2 at the level of the primitive streak . ( 3 ) SCL and Lmo 2 expression is restricted to presumptive hemangioblasts . ( 4 ) The up regulation of GATA 2 in newly formed blood islands is shortly followed by GATA 1 expression . ( 5 ) Lmo 2 is up regulated in blood island angioblasts thus appearing as one of the earliest markers for endothelial cell commitment . ^^^ VEGF R 2 is down regulated in hemopoietic cells prior to GATA 2 , SCL / tal 1 , Lmo 2 and GATA 1 in erythroblasts . . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| It has not yet been elucidated how the BMP 4 signaling promotes the differentiation of blood cells , but it is likely that transcription factors such as GATA 2 , SCL , LMO 2 , biklf and GATA 1 are at least involved in the initial blood program . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| GPA promoter activation is dependent on the assembly of a multifactorial complex containing SCL as well as ubiquitous ( E 47 , Sp 1 , and Ldb 1 ) and tissue specific ( LMO 2 and GATA 1 ) transcription factors . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| Combination of all trans retinoic acid and lithium chloride surmounts a retinoid differentiation block induced by expression of Scl and Rbtn 2 transcription factors in myeloid leukemia cells . ^^^ Addition of LiCl to ATRA treatment restored the capacity of both Scl and Rbtn 2 expressing cells to respond to the retinoid in a synergistic manner . ^^^ These findings suggest that if Scl and / or Rbtn 2 are involved in the non responsiveness of AML patients to ATRA induced differentiation , addition of LiCl may reverse insensitivity . . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| This review describes three examples of this general paradigm of leukaemogenesis : RUNX 1 abnormalities in acute leukaemias , GATA 1 mutations in the leukaemias of Down syndrome , and SCL and LMO 2 ectopic expression in T cell acute lymphoblastic leukaemia . . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| In vivo , they both bind the transcription factors GATA 1 , SCL , LMO 2 , and Ldb 1 . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| Importantly , the residue in helix 2 specifically promotes interaction with a known partner of SCL , the LIM only protein LMO 2 , a finding that strengthens genetic evidence that these proteins interact . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| To gain information on the possible involvement of this transcription factor in the non responsiveness of acute myelocytic leukemia ( AML ) patients to ATRA , we compared the endogenous expression levels of Scl and its transcription complex partners [ i . e . , Rbtn 1 ( LMO 1 ) , Rbtn 2 ( LMO 2 ) , Ldb 1 , and GATA family proteins ] in leukemic blast cells from patients with AML and acute promyelocytic leukemia ( APL ) , and determined the effects of lithium chloride alone or in combination with ATRA on the capacity of blast cells to differentiate during short term ex vivo culture . ^^^ Levels of Scl , Rbtn 2 , GATA 1 , and Ldb 1 expression were comparable in AML and APL blasts , while the levels of expression of Rbtn 1 , GATA 2 , and GATA 3 were absent or markedly lower in APL cells . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| Using gene expression analysis and visualization of vessel formation in live animals harboring an lmo 2 promoter green fluorescent protein reporter transgene ( Tg ( lmo 2 : EGFP ) ) , we show that angioblasts were specified normally in the absence of scl , but later defects in angiogenesis were evident . ^^^ Surprisingly , in cloche , lmo 2 was not induced in response to scl over expression . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : The LIM finger protein LMO 2 forms a transcription factor complex with other hematopoietic regulator proteins , such as TAL 1 ( SCL ) , LDB 1 , GATA 1 , 2 , and 3 , in the promoters of several erythroid genes . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| Maintained expression of PU . 1 gene and decreased expression of LMO 2 and SCL genes may explain the aberrant hematopoiesis in AML1 / EVI1 / + fetal liver . . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| SCL , GATA 2 and Lmo 2 expression in neurogenesis . ^^^ SCL , Lmo 2 and GATA factors form common transcription complexes during hematopoietic differentiation . ^^^ Here , we show that neural cells in the early embryonic chick mid and hindbrain express SCL and GATA 2 , while Lmo 2 is expressed only in vascular elements . ^^^ The lack of Lmo 2 transcripts in neural cells demonstrated that SCL and GATA 2 can not form common complexes with Lmo 2 in the developing brain . ^^^ In contrast to hematopoietic differentiation , however , our results failed to demonstrate co assembling of GATA factors or SCL with Lmo 2 . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| Expression of scl , gata 1 , and lmo 2 was down regulated at 12 to 14 hpf in the zMelk like gene MO injected embryos , and the coexpression of gata 1 rescued the anemic phenotype induced by zMelk like gene MO . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| Quantitative real time polymerase chain reaction showed that Scl null platelets lacked NF E 2 , and chromatin immunoprecipitation analysis demonstrated Scl binding to the NF E 2 promoter in the human megakaryoblastic cell line Meg 01 , along with its binding partners E 47 , Lmo 2 , and the cofactors Ldb 1 and GATA 2 . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| Direct interaction of the RBTN 2 protein was observed in vivo and in vitro with the GATA 1 or 2 zinc finger transcription factors , as well as with the basic helix loop helix protein TAL 1 . ^^^ By using mammalian two hybrid analysis , complexes involving RBTN 2 , TAL 1 , and GATA 1 , together with E 47 , the basic helix loop helix heterodimerization partner of TAL 1 , could be demonstrated . ^^^ Thus , a molecular link exists between three proteins crucial for erythropoiesis , and the data suggest that variations in amounts of complexes involving RBTN 2 , TAL 1 , and GATA 1 could be important for erythroid differentiation . . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| With respect to embryonic erythropoiesis , tal 1 deficiency resembles loss of the erythroid transcription factor GATA 1 or the LIM protein rbtn 2 . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| We have found that RBTN 1 and RBTN 2 have the ability to interact with each of the leukemogenic bHLH proteins ( TAL 1 , TAL 2 and LYL 1 ) . ^^^ The LIM bHLH interactions are highly specific in that RBTN 1 and RBTN 2 will associate with TAL 1 , TAL 2 and LYL 1 , but not with other bHLH proteins , including E 12 , E 47 , Id 1 , NHLH 1 , AP 4 , MAX , MYC and MyoD 1 . ^^^ Moreover , RBTN 1 and RBTN 2 can interact with TAL 1 polypeptides that exist in assembled bHLH heterodimers ( e . g . ^^^ Finally , we have identified a subset of leukemia patients that harbor tumor specific rearrangements of both their RBTN 2 and TAL 1 genes . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| The LIM protein RBTN 2 and the basic helix loop helix protein TAL 1 are present in a complex in erythroid cells . ^^^ Chromosomal translocations in T cell acute leukemias can activate genes encoding putative transcription factors such as the LIM proteins RBTN 1 and RBTN 2 and the DNA binding basic helix loop helix transcription factor TAL 1 associated with T cell acute lymphocytic leukemia . ^^^ While not expressed in normal T cells , RBTN 2 and TAL 1 are coexpressed in erythroid cells and are both important for erythroid differentiation . ^^^ We demonstrate , using anti RBTN 2 and anti TAL 1 antisera , that the LIM protein RBTN 2 is not phosphorylated and is complexed with the TAL 1 phosphoprotein in the nucleus of erythroid cells . ^^^ Since both RBTN 2 and TAL 1 are crucial for normal erythropoiesis , these data have important implications for transcription networks therein . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| Protein dimerization between Lmo 2 ( Rbtn 2 ) and Tal 1 alters thymocyte development and potentiates T cell tumorigenesis in transgenic mice . ^^^ The LMO 2 and TAL 1 genes were first identified via chromosomal translocations and later found to encode proteins that interact during normal erythroid development . ^^^ Some T cell leukaemia patients have chromosomal abnormalities involving both genes , implying that LMO 2 and TAL 1 act synergistically to promote tumorigenesis after their inappropriate co expression . ^^^ To test this hypothesis , transgenic mice were made which co express Lmo 2 and Tal 1 genes in T cells . ^^^ Dimers of Lmo 2 and Tal 1 proteins were formed in thymocytes of double but not single transgenic mice . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| We found that aberrant expression of TAL 1 detected in 11 out of 14 T ALL cell lines was invariably accompanied by that of either RBTN 1 or RBTN 2 . ^^^ Forced expression of TAL 1 together with RBTN 1 or RBTN 2 , but not TAL 1 alone , strongly induced artificial reporter genes in a TAL1 / RBTN negative T ALL cell line , HPB ALL . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| VYS erythropoiesis is decreased or abrogated by targeted disruption of the hematopoietic transcription factors tal 1 , rbtn 2 , GATA 1 , and GATA 2 . ^^^ This VYS expression occurred in a defined temporal sequence : tal 1 and rbtn 2 transcripts were detected earlier than the others , followed by GATA 2 and GATA 1 , and then by betaH 1 globin . ^^^ We conclude that primitive mammalian erythropoiesis arises during gastrulation through the ordered temporal expression of tal 1 , rbtn 2 , GATA 2 , and GATA 1 in a subset of extraembryonic mesoderm cells . ^^^ During the stages analyzed , tal 1 and rbtn 2 expression was also present in posterior embryonic mesoderm , while GATA 1 and GATA 2 expression was evident in extraembryonic tissues of ectodermal origin . . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| Human proerythroblasts and early erythroblasts , generated in vitro by normal adult progenitors , contain a pentamer protein complex comprising the tal 1 transcription factor heterodimerized with the ubiquitous E2A protein and linked to Lmo 2 , Ldb 1 , and retinoblastoma protein ( pRb ) . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| ES cell derived hematopoietic precursor cells express the cell surface antigen CD 34 and the hematopoietic transcription factors TAL 1 , LMO 2 , and GATA 2 . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| Our data support the involvement of 2 distinct mechanisms : translocations involving LMO 2 , TAL 2 , and TAL 1 in T cell acute lymphoblastic leukemia ( T ALL ) , are compatible with illegitimate 5 ( D ) J recombination between a TCR locus and a proto oncogene locus bearing a fortuitous but functional recombination site ( type 1 ) ; in contrast , translocations involving BCL 1 and BCL 2 in B cell non Hodgkin ' s lymphomas ( B NHL ) , are compatible with a process in which only the IgH locus breaks are mediated by 5 ( D ) J recombination ( type 2 ) . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| Commitment of hematopoietic cells to the erythroid lineage involves the actions of several transcription factors , including TAL 1 , LMO 2 , and GATA 2 . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| Here we show that five different T cell oncogenes ( HOX 11 , TAL 1 , LYL 1 , LMO 1 , and LMO 2 ) are often aberrantly expressed in the absence of chromosomal abnormalities . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| High expression of LYL 1 , LMO 2 , or TAL 1 was observed mainly in samples negative for HOX11L2 expression . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| The transcription factor LMO 2 is believed to exert its effect through the formation of protein protein interactions with other DNA binding factors such as GATA 1 and TAL 1 . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| Since it has been shown that TAL 1 forms complex with LMO proteins in erythroid and leukemic cells we investigated the capacity of HEN 1 to form complex with LMO 2 and LMO 4 . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| We show here that a nuclear complex containing TAL 1 , its DNA binding partner E 47 , zinc finger transcription factor GATA 1 , LIM domain protein LMO 2 , and LIM domain binding protein Ldb 1 transactivates the protein 4 . 2 ( P4 . 2 ) gene through two E box GATA elements in its proximal promoter . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| Five different multistep molecular pathways have been identified that lead to T ALL , involving activation of different T ALL oncogenes : ( 1 ) HOX 11 , ( 2 ) HOX11L2 , ( 3 ) TAL 1 plus LMO1 / 2 , ( 4 ) LYL 1 plus LMO 2 , and ( 5 ) MLL ENL . ^^^ Gene expression studies indicate activation of a subset of these genes HOX 11 , TAL 1 , LYL 1 , LMO 1 , and LMO 2 in a much larger fraction of T ALL cases than those harboring activating chromosomal translocations . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| To directly compare the effect of different cis regulatory sequences on recombination , we adapted our SV 40 based model system to the analysis of correspondingly selected bcrs from the TAL 1 , LMO 2 , retinoic acid receptor alpha ( RARalpha ) and MLL genes . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| We investigated the prognostic effect of the expression levels of eight oncogenic transcription factors TLX 1 ( HOX 11 ) , TLX 3 ( HOX11L2 ) , TAL 1 , TAL 2 , LYL 1 , OLIG 2 ( BHLHB 1 ) , LMO 1 , and LMO 2 in 52 adults with T cell acute lymphoblastic leukaemia . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| LMO 2 was more frequently coexpressed with LYL 1 , predominantly in IM0 / delta / gamma adult cases , than with TAL 1 . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| In addition to known TAL 1 partners , GATA 1 , E2A , HEB , LMO 2 and Ldb 1 , we identify the ETO 2 repressor as a novel component recruited to TAL 1 complexes through interaction with E2A / HEB . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| From 24 hpf , morphants displayed an expansion of the ventral mesoderm derived ICM , as evidenced by expansion of tal 1 , lmo 2 and crim 1 itself . ^^^ |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17542 and P25791 |
Pubmed |
SVM Score :0.0 |
| NA |
|