| Interacting proteins: P15976 and P25791 |
Pubmed |
SVM Score :0.8778398 |
| We now show that in erythroid cells Lmo 2 forms a novel DNA binding complex , with GATA 1 , TAL 1 and E2A , and the recently identified LIM binding protein Ldb1 / NLI . 0.8778398^^^ |
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| Interacting proteins: P15976 and P25791 |
Pubmed |
SVM Score :0.0 |
| With respect to embryonic erythropoiesis , tal 1 deficiency resembles loss of the erythroid transcription factor GATA 1 or the LIM protein rbtn 2 . ^^^ |
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| Interacting proteins: P15976 and P25791 |
Pubmed |
SVM Score :0.0 |
| LIM only protein Lmo 2 forms a protein complex with erythroid transcription factor GATA 1 . ^^^ |
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| Interacting proteins: P15976 and P25791 |
Pubmed |
SVM Score :0.0 |
| Association of erythroid transcription factors : complexes involving the LIM protein RBTN 2 and the zinc finger protein GATA 1 . ^^^ Direct interaction of the RBTN 2 protein was observed in vivo and in vitro with the GATA 1 or 2 zinc finger transcription factors , as well as with the basic helix loop helix protein TAL 1 . ^^^ By using mammalian two hybrid analysis , complexes involving RBTN 2 , TAL 1 , and GATA 1 , together with E 47 , the basic helix loop helix heterodimerization partner of TAL 1 , could be demonstrated . ^^^ Thus , a molecular link exists between three proteins crucial for erythropoiesis , and the data suggest that variations in amounts of complexes involving RBTN 2 , TAL 1 , and GATA 1 could be important for erythroid differentiation . . ^^^ |
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| Interacting proteins: P15976 and P25791 |
Pubmed |
SVM Score :0.0 |
| This shows a pivotal role for a LIM domain protein in lineage specification during mammalian development and suggests that RBTN 2 and GATA 1 are critical at similar stages of erythroid differentiation . . ^^^ |
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| Interacting proteins: P15976 and P25791 |
Pubmed |
SVM Score :0.0 |
| VYS erythropoiesis is decreased or abrogated by targeted disruption of the hematopoietic transcription factors tal 1 , rbtn 2 , GATA 1 , and GATA 2 . ^^^ This VYS expression occurred in a defined temporal sequence : tal 1 and rbtn 2 transcripts were detected earlier than the others , followed by GATA 2 and GATA 1 , and then by betaH 1 globin . ^^^ We conclude that primitive mammalian erythropoiesis arises during gastrulation through the ordered temporal expression of tal 1 , rbtn 2 , GATA 2 , and GATA 1 in a subset of extraembryonic mesoderm cells . ^^^ During the stages analyzed , tal 1 and rbtn 2 expression was also present in posterior embryonic mesoderm , while GATA 1 and GATA 2 expression was evident in extraembryonic tissues of ectodermal origin . . ^^^ |
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| Interacting proteins: P15976 and P25791 |
Pubmed |
SVM Score :0.0 |
| Likewise , the level of expression of the erythroid transcription factors GATA 1 , GATA 2 , NF E 2 , SCL and RBTN 2 , all expressed in untreated K 562 cells , did not increase during sodium phenylacetate induced erythroid differentiation . ^^^ |
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| Interacting proteins: P15976 and P25791 |
Pubmed |
SVM Score :0.0 |
| The composite motif binds a multi protein hematopoietic specific transcription factor complex which includes GATA 1 , SCL / tal 1 , E2A , Lmo 2 and Ldb 1 . ^^^ |
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| Interacting proteins: P15976 and P25791 |
Pubmed |
SVM Score :0.0 |
| SCL and GATA 1 interact in a transcriptional complex with the LIM domain protein LMO 2 . ^^^ Furthermore , co expression of GATA 1 with SCL and LMO 2 leads to embryos that are ventralized and have blood throughout the dorsal ventral axis . ^^^ The synergistic effect of SCL , LMO 2 and GATA 1 , taken together with the findings that these factors can form a complex in vitro , suggests that this complex specifies mesoderm to become blood during embryogenesis . . ^^^ |
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| Interacting proteins: P15976 and P25791 |
Pubmed |
SVM Score :0.0 |
| Several of them like AP 4 , GATA 1 , Lmo 2 , CEBPB , MZF 1 , NF AT , etc . were present more than once in this region . ^^^ |
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| Interacting proteins: P15976 and P25791 |
Pubmed |
SVM Score :0.0 |
| For example , the hematopoietic transcription factor GATA 1 interacts with a multitude of other nuclear proteins , including Friend of GATA 1 ( FOG 1 ) , EKLF , CBP / p300 , and Lmo 2 . ^^^ |
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| Interacting proteins: P15976 and P25791 |
Pubmed |
SVM Score :0.0 |
| The transcription factor LMO 2 is believed to exert its effect through the formation of protein protein interactions with other DNA binding factors such as GATA 1 and TAL 1 . ^^^ |
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| Interacting proteins: P15976 and P25791 |
Pubmed |
SVM Score :0.0 |
| The study includes transcription factors which are known to participate in multimeric complexes : GATA 1 , 2 , SCL / tal 1 and Lmo 2 ( whose avian orthologue we have cloned ) , VEGF R 2 , a critical regulator of hemopoietic and endothelial commitment , and hemoglobin used as a marker of the last step in erythroid differentiation . ^^^ Several findings were unexpected . ( 1 ) Two distinct patterns were revealed for GATA 2 , first : low expression , ubiquitous in all mesodermal cells , as soon as cells ingress through the primitive streak ; secondly : high , blood island specific expression . ( 2 ) VEGF R 2 is coexpressed with GATA 2 at the level of the primitive streak . ( 3 ) SCL and Lmo 2 expression is restricted to presumptive hemangioblasts . ( 4 ) The up regulation of GATA 2 in newly formed blood islands is shortly followed by GATA 1 expression . ( 5 ) Lmo 2 is up regulated in blood island angioblasts thus appearing as one of the earliest markers for endothelial cell commitment . ^^^ VEGF R 2 is down regulated in hemopoietic cells prior to GATA 2 , SCL / tal 1 , Lmo 2 and GATA 1 in erythroblasts . . ^^^ |
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| Interacting proteins: P15976 and P25791 |
Pubmed |
SVM Score :0.0 |
| It has not yet been elucidated how the BMP 4 signaling promotes the differentiation of blood cells , but it is likely that transcription factors such as GATA 2 , SCL , LMO 2 , biklf and GATA 1 are at least involved in the initial blood program . ^^^ |
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| Interacting proteins: P15976 and P25791 |
Pubmed |
SVM Score :0.0 |
| We show here that a nuclear complex containing TAL 1 , its DNA binding partner E 47 , zinc finger transcription factor GATA 1 , LIM domain protein LMO 2 , and LIM domain binding protein Ldb 1 transactivates the protein 4 . 2 ( P4 . 2 ) gene through two E box GATA elements in its proximal promoter . ^^^ |
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| Interacting proteins: P15976 and P25791 |
Pubmed |
SVM Score :0.0 |
| Lmo 2 and Scl / Tal1 convert non axial mesoderm into haemangioblasts which differentiate into endothelial cells in the absence of Gata 1 . ^^^ This restriction correlates well with activation of gata 1 transcription and co injection of gata 1 mRNA along with scl / tal1 and lmo 2 induces erythropoiesis more broadly without ventralising or posteriorising the embryo . ^^^ |
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| Interacting proteins: P15976 and P25791 |
Pubmed |
SVM Score :0.0 |
| This region contains several putative transcription factor binding sites like AP 1 , AP 4 , GATA 1 , Lmo 2 , NF kappa B , MZF 1 , NF AT , etc . ^^^ |
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| Interacting proteins: P15976 and P25791 |
Pubmed |
SVM Score :0.0 |
| GPA promoter activation is dependent on the assembly of a multifactorial complex containing SCL as well as ubiquitous ( E 47 , Sp 1 , and Ldb 1 ) and tissue specific ( LMO 2 and GATA 1 ) transcription factors . ^^^ |
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| Interacting proteins: P15976 and P25791 |
Pubmed |
SVM Score :0.0 |
| This review describes three examples of this general paradigm of leukaemogenesis : RUNX 1 abnormalities in acute leukaemias , GATA 1 mutations in the leukaemias of Down syndrome , and SCL and LMO 2 ectopic expression in T cell acute lymphoblastic leukaemia . . ^^^ |
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| Interacting proteins: P15976 and P25791 |
Pubmed |
SVM Score :0.0 |
| In vivo , they both bind the transcription factors GATA 1 , SCL , LMO 2 , and Ldb 1 . ^^^ |
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| Interacting proteins: P15976 and P25791 |
Pubmed |
SVM Score :0.0 |
| Levels of Scl , Rbtn 2 , GATA 1 , and Ldb 1 expression were comparable in AML and APL blasts , while the levels of expression of Rbtn 1 , GATA 2 , and GATA 3 were absent or markedly lower in APL cells . ^^^ |
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| Interacting proteins: P15976 and P25791 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : The LIM finger protein LMO 2 forms a transcription factor complex with other hematopoietic regulator proteins , such as TAL 1 ( SCL ) , LDB 1 , GATA 1 , 2 , and 3 , in the promoters of several erythroid genes . ^^^ |
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| Interacting proteins: P15976 and P25791 |
Pubmed |
SVM Score :0.0 |
| Expression of scl , gata 1 , and lmo 2 was down regulated at 12 to 14 hpf in the zMelk like gene MO injected embryos , and the coexpression of gata 1 rescued the anemic phenotype induced by zMelk like gene MO . ^^^ |
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| Interacting proteins: P15976 and P25791 |
Pubmed |
SVM Score :0.0 |
| In addition to known TAL 1 partners , GATA 1 , E2A , HEB , LMO 2 and Ldb 1 , we identify the ETO 2 repressor as a novel component recruited to TAL 1 complexes through interaction with E2A / HEB . ^^^ |
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| Interacting proteins: P15976 and P25791 |
Pubmed |
SVM Score :0.0 |
| We recently established that a DNA binding complex containing TAL1 / SCL , E 47 , GATA 1 , LMO 2 and Ldb 1 stimulates P4 . 2 ( protein 4 . 2 ) transcription in erythroid progenitors via two E box GATA elements in the gene ' s proximal promoter . ^^^ |
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| Interacting proteins: P15976 and P25791 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15976 and P25791 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15976 and P25791 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15976 and P25791 |
Pubmed |
SVM Score :0.0 |
| NA |
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