| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :1.2976749 |
| Using recombinant proteins in conjunction with immunoprecipitation and immunoblotting , we show that Gadd 45 competes with p 21 for binding to PCNA and conversely , p 21 blocks the ability of Gadd 45 to bind PCNA . 1.2976749^^^ Here we demonstrate that Gadd 45 can directly bind to PCNA using a Far western blotting approach . 0.90935577^^^ Direct interaction of Gadd 45 with PCNA and evidence for competitive interaction of Gadd 45 and p21Waf1 / Cip1 with PCNA . 0.89198303^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.60461696 |
| It is postulated that the interactions of Gadd 45 with both p21Cip1 and PCNA are important for the modulation of cell cycles , and for the inhibition of DNA replication . . 0.60461696^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.73480323 |
| Gadd 45 was found to bind to PCNA , a normal component of Cdk complexes and a protein involved in DNA replication and repair . 0.73480323^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.78894949 |
| Our results confirm an interaction between PCNA and Gadd 45 , define regions of both molecules involved in this interaction , and are consistent with a potential stoichiometry of 2 Gadd 45 molecules to each PCNA monomer . 0.78894949^^^ We used a yeast two hybrid assay to demonstrate that human Gadd 45 interacts with both human and S . pombe PCNA . 0.53331975^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.64201636 |
| GADD 45 can interact in vivo with the G ( 2 ) cell cycle specific kinase , Cdc 2 , proliferating cell nuclear antigen ( PCNA ) , and the cell cycle kinase inhibitor p 21 ( waf 1 ) . 0.64201636^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.9787173 |
| Gadd 45 protein has been shown to interact with PCNA and also p21waf1 . 0.9787173^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.53737445 |
| Gadd45a protein has been reported to interact with multiple important cellular proteins , including Cdc 2 protein kinase , proliferating cell nuclear antigen ( PCNA ) , p21Waf1 / Cip1 protein , core histone protein and MTK / MEKK4 , an up stream activator of the JNK / SAPK pathway , indicating that Gadd45a may play important roles in the control of cell cycle checkpoint , DNA repair process , and signaling transduction . 0.53737445^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| A transcriptional target of p 53 , Gadd 45 , was recently found to bind to PCNA , a component of DNA replication / repair complexes , thereby implicating Gadd 45 in DNA metabolism . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| In cells exposed to 3 6 Gy low dose rate gamma irradiation we observed increased levels of the GADD 45 transcript and lower transcript levels of the genes TOPOISOMERASE 2 alpha , FACC , CYCLIN A and CYCLIN B . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| The differentiation primary response gene MyD 118 , related to GADD 45 , encodes for a nuclear protein which interacts with PCNA and p21WAF1 / CIP1 . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| With concentrations of each inhibitor able to block DNA synthesis , no induction of message for the cyclin dependent kinase inhibitor waf 1 was observed ; while induction of gadd 45 message indicated that the cells might be responding to growth arrest or DNA damage . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| The p 53 regulated cyclin G gene promotes cell growth : p 53 downstream effectors cyclin G and Gadd 45 exert different effects on cisplatin chemosensitivity . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| OP also differentially induces endogenous expression of several known p 53 target genes such as Waf 1 and Mdm 2 , but not Bax , Gadd 45 , and PCNA . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| The expression of the p 21 ( waf1 / cip1 / sdi1 ) , cyclin G and gadd 45 genes was increased ( about 3 to 4 fold that at 37 degrees C ) , when the cells were cultured at 32 degrees C . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| The immunoreactive expression of wt p 53 , p 53 response proteins ( WAF 1 , Bax and Gadd 45 ) , and a cell cycle protein ( cyclin D ) were detected and preferentially localized to nuclei of apoptotic granule cells , and were weakly expressed in nuclei of necrotic pyramidal CA1 / 2 cells . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| The genes coding p 21 , GADD 45 , mdm 2 , cyclin G etc . contain such a p 53 responsive element . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| We used double staining histochemistry to investigate the relationship between apoptotic cell death and selective protein expression associated with DNA damage ( p 53 , Bax , MDM 2 , Gadd 45 ) , DNA repair ( PCNA ) and cell cycle proteins ( cyclin A , cyclin D , cdk 2 , cdk 4 ) in rats ( n = 6 ; control rats , n = 5 ) subjected to transient ( 2 h ) middle cerebral artery occlusion ( MCAo ) and 46 h of reperfusion . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Association with the growth arrest and DNA damage inducible proteins gadd 45 and MyD 118 , further demonstrates the role of PCNA as a component of the cell cycle control apparatus . . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| The eukaryotic polymerase processivity factor , PCNA , interacts with cell cycle regulatory proteins such as p 21 ( WAF1 / Cip1 ) and Gadd 45 , as well as with proteins involved in the mechanics of DNA repair and replication . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| However , we detected induction of the p21Cip1 cyclin dependent kinase inhibitor gene during differentiation , which was markedly enhanced in the presence of p 53 . p 53 dependent expression of p21Cip1 occurred in the absence of an increase in p 53 mRNA and protein levels and was specific for p21Cip1 , since expression of gadd 45 , mdm 2 , cyclin G and bax were unaffected by p 53 . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Double staining immunohistochemistry was also used to investigate the relationship between apoptotic cell death and selective protein expression associated with DNA damage and repair ( p 53 , Bax , MDM 2 , WAF 1 , Gadd 45 , PCNA ) and cell cycle protein , Cyclin D 1 , in male Wistar rats 48 h after injury . ^^^ Proteins associated with DNA damage and repair ( p 53 , Bax , MDM 2 , WAF 1 , Gadd 45 , PCNA ) were expressed in the cytoplasm of normal cells of naive and sham rats . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| UV radiation is a transcriptional inducer of p 21 ( Cip1 / Waf1 ) cyclin kinase inhibitor in a p 53 independent manner . p 53 target genes p 21 ( Cip1 / Waf1 ) cyclin kinase inhibitor ( p 21 CKI ) , GADD 45 , bax , and cyclin G and genes affecting the redox state of the cells are implicated in p 53 damage control responses . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Increased expression of cyclin B 1 and Cdc25C inhibited the Gadd 45 mediated G2 / M arrest in human fibroblasts , indicating that the mechanism of Gadd 45 mediated G2 / M checkpoint is at least in part through modulation of the activity of the G 2 specific kinase , cyclin B1 / p34 ( cdc 2 ) . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| In this process , p 53 recovered the wild type p 53 function and the expression of the p 21 ( waf1 / cip1 / sdi1 ) , cyclin G 1 and gadd 45 genes was increased . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Association with Cdc 2 and inhibition of Cdc2 / Cyclin B 1 kinase activity by the p 53 regulated protein Gadd 45 . ^^^ While inhibitory phosphorylation of Cdc 2 and suppression of cyclin B 1 levels are known to be involved in G 2 delays after genotoxic stress , Gadd 45 has now been found to directly inhibit the activity of Cdc2 / Cyclin B 1 complex , while it had no appreciable effect on Cdk2 / Cyclin E activity even at very high levels of Gadd 45 . ^^^ Gadd 45 was also able to physically interact with Cdc 2 , but not Cyclin B 1 . ^^^ Addition of Gadd 45 to immunoprecipitated Cdc2 / Cyclin B 1 in vitro led to a dissociation of this complex , and thus may represent a new checkpoint mechanism whereby Cdc2 / Cyclin B 1 can be inhibited . ^^^ With the use of an antisense approach , reduced Gadd 45 expression attenuated the suppression of Cdc2 / Cyclin B 1 activity in UV irradiated human cells . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Several other genes observed to be differentially expressed by homocysteine are known to mediate cell growth and differentiation ( ie , GADD 45 , GADD 153 , Id 1 , cyclin D 1 , FRA 2 ) , a finding that supports the observation that homocysteine causes a dose dependent decrease in DNA synthesis in HUVEC . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Notable changes included induction of the GADD 45 and GADD 153 genes and a reduction in cyclin B 1 expression . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Characterization of MyD 118 , Gadd 45 , and proliferating cell nuclear antigen ( PCNA ) interacting domains . ^^^ PCNA impedes MyD 118 AND Gadd 45 mediated negative growth control . ^^^ MyD 118 and Gadd 45 are related proteins that previously were shown to interact with proliferating cell nuclear antigen ( PCNA ) , implicated in DNA replication , DNA repair , and cell cycle progression . ^^^ To establish the role of MyD 118 and Gadd 45 interactions with PCNA , in this work we sought to identify the interacting domains and analyze the significance of this interaction in negative growth control . ^^^ Using complementary in vivo and in vitro interaction assays the N terminal ( 1 46 ) and middle ( 100 127 ) regions of PCNA were identified as harboring MyD 118 and Gadd 45 interacting domains , whereas PCNA interacting domains within MyD 118 and Gadd 45 were localized to the C termini of these proteins ( amino acids 114 156 and 137 165 , respectively ) . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| These include genes involved in DNA replication and repair ( e . g . , PCNA , XRCC 1 , B MYB , and GADD 45 ) , transcriptional regulators associated with stress response , and cell cycle checkpoint control ( e . g . , YB 1 , DBPA , and ATF 4 ) , and genes for signal transduction proteins ( e . g . , protein tyrosine phosphatase 1B and regulatory subunits alpha and beta of cAMP dependent protein kinase ) . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Recently , we have shown that both MyD 118 and GADD 45 interact with proliferating cell nuclear antigen ( PCNA ) , a protein that plays a central role in DNA replication , DNA repair , and cell cycle progression , as well as with the universal cyclin dependent kinase inhibitor p 21 . ^^^ Moreover , it is shown that CR 6 interacts with PCNA via a domain that also mediates interaction of both GADD 45 and MyD 118 with PCNA . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| PCR analysis confirmed HO 1 , p 21 ( waf1 / cip1 ) , HSP 70 , GPX 1 , GADD 45 , QR , mdr 1 , PGHS , and cyclin D 1 changes . ^^^ Increases occurred in oxidative stress responsive genes HO 1 , QR , HSP 70 , GADD 45 , GADD 153 , p 21 ( WAF1 / CIP16 ) , GST ' s , GAPDH , TPX , and GPX 1 ( 0 ) ; transcriptional regulators c jun , c fos , jun B , c myc , and IkappaB ; protein repair components Rdelta , RC 10 2 , C 3 , RC 7 , HR6B ubiquitin conjugating enzyme and ubiquitin ; DNA repair components PCNA , msh 2 , and O 6 methylguanine DNA methyltransferase ; lipid peroxide excision enzyme PLA 2 ; and apoptogenic components TNFalpha , iNOS 1 and FasL . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Indeed , like p 53 , p 73 as well ( 1 ) can bind mdmX , mdm 2 , p300 / CAF and adenovirus E 4 orf6 proteins , ( 2 ) can trigger several promoters including p 21 , bax , mdm 2 , gadd 45 , cyclin G , IGFBP 3 , 14 3 3 sigma , ( 3 ) is able to trigger cell death , ( 4 ) is involved in the DNA damage response , although through a different pathway . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| However , glutathione S transferase fused and thus dimerized p 63 and p 53 core domains had similar affinity and specificity for the p 53 consensus sites p 21 , gadd 45 , cyclin G , and bax . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| These results indicated that during the initiation stage , MNNG induced G2 / M arrest and apoptosis with increased expressions of wild type p 53 , p 27 , and GADD 45 proteins ; and down regulated mRNA level of cyclin B 1 and up regulated mRNA level of cyclin G . ^^^ To determine the alterations of cell cycle control in the MNNG induced initiation stage during transformation , we examined the effects of MNNG on Balb / 3T3 A 31 cell growth , and determined the alterations of the protein and / or mRNA levels of cyclins B 1 , D 1 , E , and G , PCNA , GADD 45 , p 27 , and wild type p 53 . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Western blot analysis and immunohistochemistry were performed to examine the expression of p 53 , proliferating cell nuclear antigen , Bax , Bcl 2 , p21WAF1 / Cip1 and Gadd 45 . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| The PCNA gene is induced by p 53 , while PCNA protein interacts with p 53 controlled proteins Gadd 45 , MyD 118 , CR 6 and , most importantly , p 21 , in the process of deciding cell fate . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| In the present study , we expanded our investigations to examine the effect of cisplatin induced ERK activation on the expression of p 53 targeted genes that have been shown to be important in the cellular response to DNA damage including Bax , Bcl 2 , Bcl x 1 , Cyclin G , Gadd 45 , p21WAF1 , and Mdm 2 . ^^^ In the ovarian carcinoma cell line A 2780 , cisplatin was shown to induce expression of p21WAF1 , Gadd 45 and Mdm 2 , but cisplatin had no effect on expression of Bax , Bcl 2 , Bcl x 1 , or Cyclin G . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Examination of mRNA levels of p 53 regulated genes involved in cell cycle control ( p 21 , gadd 45 , and cyclin G ) or apoptosis ( bax and bcl 2 ) showed that during chronic genotoxic stress , bone marrow cells from p53+ / + mice expressed significantly higher levels of a majority of these genes compared to p53+ / bone marrow cells . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Ribonuclease protection assays compared the effects of acetaminophen and E 2 on expression of selected genes ( c myc , c fos , cyclin D 1 , bcl 2 , bax , gadd 45 , mcl 1 , p 53 , p 21 ( CIP1 / WAF1 ) , and bcl xL ) in E 2 responsive breast cancer ( MCF 7 ) and endometrial adenocarcinoma ( Ishikawa ) cells as well as in E 2 nonresponsive ( MDA MB 231 ) breast cancer cells . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| A semi quantitative RT PCR method was developed to allow the analysis of the expression of 5 p 53 effector genes ( p 21 ( WAF 1 ) , mdm 2 , cyclin G 1 , gadd 45 , bax ) at the same time with a small amount of RNA ( 1 microg ) . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| An oligonucleotide microarray analysis demonstrated that , in addition to the heat shock responsive and redox responsive genes , the p 53 responsive genes , such as gadd 45 , cyclin G 1 , and cathepsin D , were significantly up regulated in the cells treated with 15d PGJ ( 2 ) . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| For this we studied the effect of CA4P on selected markers of apoptosis [ caspases 9 and 3 , poly ( ADP ribose ) polymerase ( PARP ) , bcl 2 , and bax ] and G 2 M protein regulators ( p 53 , MDM 2 , 14 3 3sigma , GADD 45 , cdc 2 , cdc 25 , chk 1 , wee 1 , p 21 , and cyclin B 1 ) . ^^^ Exposure of WSU CLL cells to 4 and 5 nM CA4P was associated with overproduction of total p 53 and no dramatic change in MDM 2 , 14 3 3sigma , GADD 45 , the cyclin dependent kinase cdc 2 , its inhibitory phosphorylation , the cdc 2 inhibitory kinase ( wee 1 ) , chk 1 , or cdc 25 hyperphosphorylation . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Expression of p 53 , p 21 , Bcl 2 , GADD 45 , cyclin D , phospho MAP kinase , cdk 2 , and Akt 1 at various concentrations of BCA in cultured hRPE cells was examined by immunoblot analysis . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Mechanisms of 5 azacytidine ( 5AzC ) induced toxicity in the rat foetal brain were investigated . 5AzC ( 10 mg / kg ) was injected into pregnant rats on day 13 of gestation and the protein and mRNA expressions of p 53 and its transcriptional target genes , p 21 , bax , cyclin G 1 , fas , and gadd 45 , were examined in the foetal brain . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Cyclin D 1 , P 21 ( WAF1 / CIP1 ) , GADD 45 and PCNA protein expression were detected by Western blotting and the RT PCR method was applied to study the expression of Cdk 4 . ^^^ RESULTS : Cyclin D 1 , Cdk 4 and PCNA expressions were significantly inhibited , and the expression of P 21 ( WAF1 / CIP1 ) and GADD 45 were significantly induced by tea polyphenols and tea pigments treatments . ^^^ Cyclin D 1 , P 21 ( WAF1 / CIP1 ) , GADD 45 and PCNA protein expression were detected by Western blotting and the RT PCR method was applied to study the expression of Cdk 4 . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| GADD 45 induced cell cycle G 2 M arrest associates with altered subcellular distribution of cyclin B 1 and is independent of p 38 kinase activity . ^^^ Importantly , induction of Gadd 45 protein resulted in a reduction of nuclear cyclin B 1 protein , whose nuclear localization is critical for the completion of G 2 M transition . ^^^ Additionally , overexpression of cyclin B 1 substantially abrogated the GADD 45 induced cell growth suppression . ^^^ Therefore , GADD 45 inhibition of Cdc 2 kinase activity through alteration of cyclin B 1 subcellular localization may be an essential step in the GADD 45 induced cell cycle G 2 M arrest and growth suppression . . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| In vitro , rofecoxib decreased gene expression of cyclin D1 / PRAD1 , a key component of cell cycle progression , while increasing expression of several cell cycle arrest genes , including p21 / WAF1 , p33 / ING , GADD 34 , and GADD 45 ( P < 0 . 05 ) . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| DES induced the expression of 12 genes ( bad , bax , bcl 10 , caspase 1 , p 53 , cyclin D 3 , GADD 45 , p 21 , p 15 , p 27 , p 57 and Skp 1 ) and down regulated the expression of eight genes ( bcl 2 , caspase 2 , caspase 7 , caspase 8 , E 124 , iNOS , mdm 2 and NFkappab 1 ) at twofold or greater levels . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Recent evidences from several laboratories suggest that BRCA 1 may regulate the expression of many genes like p 21 ( WAF1 / CIP1 ) , Gadd 45 , Cyclin B 1 , DBB 2 , XPC , 14 3 3 and others at the level of transcription . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Moreover , calcitriol mediated arrest of beta TC ( 3 ) cells in the G ( 1 ) phase of the cell cycle was associated with the abnormal expression of p 21 and G ( 2 ) / M specific cyclin B 2 genes and involved the DNA damage inducible factor GADD 45 . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| We also identified 19 novel genes that have neither been described in non small cell lung cancer ( i . e . , cdc 2 , cullin 4A , ZAC , p 57 , DP 1 , GADD 45 , PISSLRE , cdc 20 ) nor in any other tumors ( i . e . , cyclin F , cullin 5 , p 34 ) . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Drug treatment reduced levels of cyclin B 1 message ( which normally peaks in G2 / M phase of the cell cycle ) and induced a four to fivefold upregulation of GADD 45 message . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| XK 469 caused the activation of p 53 with up regulation of p 53 dependent proteins such as Bax , p 21 , Gadd 45 and cyclin B 1 in association with G2M arrest . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| We also determined levels of the stress proteins gadd 45 and p 16 and the stress and proliferation related protein , proliferating cell nuclear antigen ( PCNA ) . ^^^ In acute studies , telomerase activity was increased , p 16 initially decreased then normalized , PCNA levels did not change significantly even in the overnight recovery groups , and gadd 45 was decreased in some TBHP exposed groups . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Also , the inability of cell cycle regulatory proteins like PCNA , p 21 ( waf 1 ) , and Gadd 45 to control DNA demethylase activity in cancerous cell lines is discussed in terms of accelerated G ( 1 ) / G ( 2 ) cell cycle stage exit to facilitate unregulated cellular proliferation , loss of control of chromosomal organization , and the development of oncogenesis in cancerous cell lines . . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Diploid human lung fibroblasts were incubated with 3 9 uM Na2CrO4 , and RNA was isolated at 4 , 8 , and 24 h , as well as 24 h after Cr ( 6 ) exposure was terminated ( recovery ) . mRNA expression was quantitated by RNase protection assay with a 32P labeled multi transcript probe containing gene sequences for the cdk inhibitors , p21waf1 / cip1 , p27kip1 , p16INK4a , p15INK4b ; the pro apoptotic proteins bcl XS and bax ; the anti apoptotic proteins bcl W , bcl XL , and bcl 2 , GADD 45 , and cyclin A . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| RT PCR analysis showed that the expression of mRNAs from the mdr 1 , MRP , PCNA , topoisomerase 2 alpha and beta , GSTpi , p 21 and GADD 45 genes was not altered in the salvicine resistant subline . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Levels of PCNA and gadd 45 proteins involved in DNA repair in response to genomic damage were increased , suggesting that the cells were responding to CSC induced genomic damage . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Western blotting was used for caspase 3 and PARP , caspase 7 , caspase 9 , cytochrome c , Bcl 2 , Bax , Mcl 1 , cyclinA , cyclin B 1 , cyclin D 1 , cyclin E , CDK 2 , CDK 4 , CDK 6 , P 21 , P 27 , GADD 45 , GADD 153 . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| The level of p 53 , p21Cip1 , bax , mdm 2 , cyclin G , gadd 45 genes expression in the liver of mice of different lines given OAT injection was studied by multiplex PCR method . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| The amino terminus of p 21 interacts with cyclins and cyclin dependent kinases , while the carboxyl terminus interacts with proliferating cell nuclear antigen ( PCNA ) , growth arrest and DNA damage inducible gene 45 ( GADD 45 ) , calmodulin , SET , and CCAAT / enhancer binding protein alpha ( C / EBP alpha ) . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| The expressions of cyclin dependent kinase inhibitors ( p 21 and p 27 ) , cyclin B 1 , cyclin D 1 , PCNA , GADD 45 , and GADD 153 were significantly increased in the outer medulla , reaching peak levels at 3 days after CDDP . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Co incubation with pifithrin alpha ( PFT alpha ) , a selective p 53 inhibitor , restored GADD 45 , Bcl 2 , cyclin B 1 , and p 21 ( WAF 1 ) expression levels and almost completely reversed the growth inhibitory , cell cycle , and apoptotic effects of 5 aza CR . 5 aza CR treatment caused global demethylation and reactivation of p 16 ( INK 4 ) expression . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Furthermore , we showed that treatment of these cells with both drugs downregulated cyclin B 1 and Cdc 2 expression , but elevated the levels of p 53 , p21waf1 / cip1 , p27kip1 and Gadd 45 . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Between borderline and malignant tumors , the increased expression levels of P 53 , Bax , Cyclin E , and cyclin dependent kinase 2 as well as the decreased expression levels of growth arrest and DNA damage ( GADD 45 ) and murine double minute 2 ( MDM 2 ) were significantly associated with malignancy ( P < 0 . 01 , each ) . ^^^ In addition , the P 53 , GADD 45 , Cyclin E , and MDM 2 expression levels in malignant ovarian tumors might be useful for determining the histologic grade and type . . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Gadd 45 deficient keratinocytes fail to repair UV induced DNA damage , but the mechanism by which Gadd 45 stimulates repair of UV induced DNA damage is unknown . p21WAF1 / Cip1 ( p 21 ) is a well characterized downstream target of p 53 that binds to Gadd 45 and proliferating cell nuclear antigen ( PCNA ) . ^^^ Since a physical interplay exists between p 21 , Gadd 45 and PCNA , we hypothesized that Gadd 45 promoted DNA repair via p 21 . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| The up regulated genes include HIAP 1 , CRAF 1 , TRAF 6 , CASP 1 , CASP 2 , CASP 3 , CASP 4 , HPRT , GADD 45 , MCL 1 , NIP 1 , BCL2L2 , TRAP 3 , GSTP 1 , DAXX , PIG 11 , UBC , PIG 3 , PCNA , CDC 10 , JNK 1 and RBP 2 . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| The results showed that TCPOBOP did not activate NFB in any of the mice , but a strong induction of Gadd 45 messenger RNA was observed in all three genotypes , where TCPOBOP also induced CyP2b10 , a classical target gene of activated CAR , and cyclin D 1 , a proliferation linked gene . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Among the genes that showed altered expression patterns , some were already known to be regulated by irradiation , for instance ODC , EGR 1 , FGF 2 , PCNA , PKC , and several p 53 target genes , including p53DINP1 , BTG 2 , GADD 45 , and MDM 2 . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Among several genes related to the p 53 pathway , nutlin 3 up regulated the steady state mRNA levels of PCNA , CDKN1A / p21 , GDF 15 , TNFRSF10B / TRAIL R 2 , TP53I3 / PIG3 , and GADD 45 . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Consistently , Gadd45a has previously been shown to interact in vitro and / or in vivo with a number of proteins playing central roles in these cellular processes : proliferating cell nuclear antigen , p 21 ( Cip1 / Waf1 ) , Cdc 2 CyclinB complex , MTK 1 , and histones . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Microarray analyses revealed changes in the expression of a small number of cell cycle related genes ( p 21 , GADD45A , cyclin G 2 , MCM 5 , and histones ) that suggested their putative involvement in ET 743 induced cell cycle arrest . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Quantitative reverse transcription polymerase chain reaction verified mRNA changes in this group , which included gadd45a , c myc , cyclin D 1 and cdk 4 , pcna , cyclin G , Rb , and E2F5 . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Gadd45a interacts with Cdc 2 , dissociates the Cdc 2 cyclin B 1 complex , alters cyclin B 1 nuclear localization , and thus inhibits the activity of Cdc2 / cyclin B 1 kinase . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| However , 0 . 4 microg / kg bw TCDD also altered the expression of Gadd45a and Cyclin D 1 , suggesting that even low dose TCDD exposure can alter the expression of genes indicative of cellular stress or DNA damage and associated with cell cycle control . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| Resveratrol increased ( + ) and decreased ( ) gene expression associated with apoptosis ( Birc5+ , Cash+ , Mcl 1+ , Mdm2+ , Rpa like+ ) , cellular proliferation ( Ctsd+ , Mdm2+ , Egr1+ , ODC+ ) and cell cycle ( cyclin D+ , cyclin g+ , Gadd45a , Mad2l , Mdm2+ ) . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| RESULTS : 5 FU treatment for 24 hours resulted in S phase arrests , p 53 accumulation , up regulation of p 53 target genes on DNA damage response ( ATF 3 , GADD 34 , GADD45A , PCNA ) , cell cycle regulatory ( CDKN1A ) , and apoptosis regulatory pathways ( FAS ) , and apoptosis induction in the parental and resistant cell lines . ^^^ |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|