| Interacting proteins: P24522 and P06493 |
Pubmed |
SVM Score :0.5915228 |
| Gadd 45 was also able to physically interact with Cdc 2 , but not Cyclin B 1 . 0.5915228^^^ |
|
| Interacting proteins: P24522 and P06493 |
Pubmed |
SVM Score :0.7149116 |
| Recent findings indicate that Gadd 45 interacts with Cdc 2 protein and inhibits Cdc 2 kinase activity . 0.7149116^^^ |
|
| Interacting proteins: P24522 and P06493 |
Pubmed |
SVM Score :0.6396786 |
| Gadd45a interacts with Cdc 2 , dissociates the Cdc 2 cyclin B 1 complex , alters cyclin B 1 nuclear localization , and thus inhibits the activity of Cdc2 / cyclin B 1 kinase . 0.6396786^^^ |
|
| Interacting proteins: P24522 and P06493 |
Pubmed |
SVM Score :0.0 |
| Increased expression of cyclin B 1 and Cdc25C inhibited the Gadd 45 mediated G2 / M arrest in human fibroblasts , indicating that the mechanism of Gadd 45 mediated G2 / M checkpoint is at least in part through modulation of the activity of the G 2 specific kinase , cyclin B1 / p34 ( cdc 2 ) . ^^^ |
|
| Interacting proteins: P24522 and P06493 |
Pubmed |
SVM Score :0.0 |
| Interestingly , the p 21 binding domain of Gadd 45 also encodes the Cdc 2 binding activity , indicating that the central region of Gadd 45 may serve as an important `` core , ' ' through which Gadd 45 protein is able to present cross talk with other cell cycle regulators . ^^^ In addition , GADD 45 inhibition of Cdc 2 kinase activity was compared with Myd 118 and CR 6 , two other members of the GADD 45 family . ^^^ GADD 45 was shown to generate the strongest inhibitory effect on Cdc 2 activity . ^^^ |
|
| Interacting proteins: P24522 and P06493 |
Pubmed |
SVM Score :0.0 |
| GADD 45 can interact in vivo with the G ( 2 ) cell cycle specific kinase , Cdc 2 , proliferating cell nuclear antigen ( PCNA ) , and the cell cycle kinase inhibitor p 21 ( waf 1 ) . ^^^ The ability of GADD 45 to induce a G ( 2 ) / M arrest may be caused in part by the inhibition of Cdc 2 kinase activity . ^^^ However , unlike GADD 45 , neither GADD45beta nor GADD45gamma inhibited the Cdc 2 kinase or induced G ( 2 ) / M arrest . ^^^ Alanine substitutions in the region abolished GADD 45 induction of a G ( 2 ) / M arrest and its inactivation of the Cdc 2 kinase but not its binding to Cdc 2 , PCNA , or p 21 ( waf 1 ) . ^^^ Therefore , the binding of GADD 45 to Cdc 2 was insufficient to induce a G ( 2 ) / M arrest , and additional activity contributed by the DEDDDR residues may be necessary to regulate the G ( 2 ) / M checkpoint . . ^^^ |
|
| Interacting proteins: P24522 and P06493 |
Pubmed |
SVM Score :0.0 |
| Cdc 2 is inhibited simultaneously by three transcriptional targets of p 53 , Gadd 45 , p 21 , and 14 3 3 sigma . ^^^ |
|
| Interacting proteins: P24522 and P06493 |
Pubmed |
SVM Score :0.0 |
| Of the differentially expressed genes encoding proteins regulating the G ( 2 ) to M transition , chk 1 , wee 1 , and GADD 45 are known to inhibit cdc 2 kinase activity . ^^^ |
|
| Interacting proteins: P24522 and P06493 |
Pubmed |
SVM Score :0.0 |
| Cdc 2 kinase activity in immunoprecipitates from normal and Gadd 45 deficient cells decreases after irradiation in normal cells but not in Gadd 45 deficient cells . ^^^ An immunofluorescent study with Cdc 2 antibody demonstrated diffuse cellular fluorescence before ultraviolet irradiation in both Gadd 45 deficient and wild type cells , but upon ultraviolet irradiation only Gadd 45 proficient cells showed Cdc 2 sequestration in the cytoplasm . ^^^ These results reveal that GADD 45 promotes G2 / M arrest via nuclear export and kinase activity of Cdc 2 , increases global genomic DNA repair , and inhibits cell death in keratinocytes . ^^^ |
|
| Interacting proteins: P24522 and P06493 |
Pubmed |
SVM Score :0.0 |
| For this we studied the effect of CA4P on selected markers of apoptosis [ caspases 9 and 3 , poly ( ADP ribose ) polymerase ( PARP ) , bcl 2 , and bax ] and G 2 M protein regulators ( p 53 , MDM 2 , 14 3 3sigma , GADD 45 , cdc 2 , cdc 25 , chk 1 , wee 1 , p 21 , and cyclin B 1 ) . ^^^ Exposure of WSU CLL cells to 4 and 5 nM CA4P was associated with overproduction of total p 53 and no dramatic change in MDM 2 , 14 3 3sigma , GADD 45 , the cyclin dependent kinase cdc 2 , its inhibitory phosphorylation , the cdc 2 inhibitory kinase ( wee 1 ) , chk 1 , or cdc 25 hyperphosphorylation . ^^^ |
|
| Interacting proteins: P24522 and P06493 |
Pubmed |
SVM Score :0.0 |
| Therefore , GADD 45 inhibition of Cdc 2 kinase activity through alteration of cyclin B 1 subcellular localization may be an essential step in the GADD 45 induced cell cycle G 2 M arrest and growth suppression . . ^^^ |
|
| Interacting proteins: P24522 and P06493 |
Pubmed |
SVM Score :0.0 |
| Morin causes growth arrest in G ( 2 ) / M , without inducing apoptosis , and this is associated with induction of GADD 45 and phosphorylation and inactivation of the cell cycle kinase , cdc 2 . ^^^ |
|
| Interacting proteins: P24522 and P06493 |
Pubmed |
SVM Score :0.0 |
| Dominant negative inhibition of p 53 in SG 231 cells significantly blocked the 15d PGJ ( 2 ) and troglitazone induced growth inhibition , G2 / M arrest , and GADD45 / p21 induction . 15d PGJ ( 2 ) and troglitazone failed to directly inhibit Cdc 2 activity in a cell free system in spite of direct association between GADD 45 and PPARgamma proteins . ^^^ |
|
| Interacting proteins: P24522 and P06493 |
Pubmed |
SVM Score :0.0 |
| We also identified 19 novel genes that have neither been described in non small cell lung cancer ( i . e . , cdc 2 , cullin 4A , ZAC , p 57 , DP 1 , GADD 45 , PISSLRE , cdc 20 ) nor in any other tumors ( i . e . , cyclin F , cullin 5 , p 34 ) . ^^^ |
|
| Interacting proteins: P24522 and P06493 |
Pubmed |
SVM Score :0.0 |
| Furthermore , we showed that treatment of these cells with both drugs downregulated cyclin B 1 and Cdc 2 expression , but elevated the levels of p 53 , p21waf1 / cip1 , p27kip1 and Gadd 45 . ^^^ |
|
| Interacting proteins: P24522 and P06493 |
Pubmed |
SVM Score :0.0 |
| Altered genes included BRCA 1 associated protein 1 [ ubiquitin carboxy terminal hydrolase ( bap 1 ) ] ; cell division cycle 2 , G 1 to S and G 2 to M [ cdk 1 ( cdc 2 ) ] ; and DNA damage inducible transcript 1 ( gadd 45 ) , which were down regulated . ^^^ |
|
| Interacting proteins: P24522 and P06493 |
Pubmed |
SVM Score :0.0 |
| Data also has been obtained that Gadd45b and Gadd45g , as well as GADD45a , interact with both Cdk 1 and cyclinB 1 , resulting in inhibition of the kinase activity of the Cdk1 / cyclinB1 complex . ^^^ |
|
| Interacting proteins: P24522 and P06493 |
Pubmed |
SVM Score :0.0 |
| Gadd45a protein has been reported to interact with multiple important cellular proteins , including Cdc 2 protein kinase , proliferating cell nuclear antigen ( PCNA ) , p21Waf1 / Cip1 protein , core histone protein and MTK / MEKK4 , an up stream activator of the JNK / SAPK pathway , indicating that Gadd45a may play important roles in the control of cell cycle checkpoint , DNA repair process , and signaling transduction . ^^^ |
|
| Interacting proteins: P24522 and P06493 |
Pubmed |
SVM Score :0.0 |
| UVB induced G 2 arrest of human melanocytes involves Cdc 2 sequestration by Gadd45a in nuclear speckles . ^^^ Knock down of Gadd45a expression by RNA interference prevents both UVB induced Cdc 2 accumulation in Nuclear Speckles and G ( 2 ) arrest . ^^^ Furthermore , we show that Cdc 2 sequestration by Gadd45a occurs in Nuclear Speckles , suggesting a new role for these nuclear bodies , so far only linked to RNA maturation . . ^^^ |
|
| Interacting proteins: P24522 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24522 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|