| Interacting proteins: P24468 and P15172 |
Pubmed |
SVM Score :0.0 |
| Identification of a regulatory function for an orphan receptor in muscle : COUP TF 2 affects the expression of the myoD gene family during myogenesis . ^^^ We show that Escherichia coli expressed full length and truncated COUP TF 2 bound in a sequence specific manner to the T 3 response elements ( TREs ) in the myoD and myogenin regulatory HLH genes [ Olson ( 1992 ) Dev . ^^^ Constitutive over expression of COUP TF 2 cDNA in mouse C2C12 myogenic cells suppressed the levels of myoD mRNA and blocked the induction of myogenin mRNA , whereas constitutive expression of anti sense COUP TF 2 cDNA significantly increased the steady state levels of myoD mRNA and hyper induced myogenin mRNA . ^^^ These studies demonstrate for the first time ( 1 ) that COUP TF 2 , functions as a physiologically relevant antagonistic regulator of myogenesis via direct effects on the myoD gene family and ( 2 ) direct evidence for the developmental role of COUP TF 2 during mammalian cell differentiation . . ^^^ |
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| Interacting proteins: P24468 and P15172 |
Pubmed |
SVM Score :0.0 |
| The orphan nuclear receptor , COUP TF 2 , inhibits myogenesis by post transcriptional regulation of MyoD function : COUP TF 2 directly interacts with p 300 and myoD . ^^^ Forced expression of the orphan nuclear receptor COUP TF 2 in mouse C 2 myogenic cells has been demonstrated to inhibit morphological differentiation , and to repress the expression of : ( 1 ) the myoD gene family which encodes myogenic basic helix loop helix ( bHLH ) proteins ; and ( 2 ) the cell cycle regulator , p 21 ( Waf 1 / Cip 1 ) . ^^^ In the present study , we show that COUP TF 2 efficiently inhibits the myoD mediated myogenic conversion of pluripotential C3H10T1 / 2 cells by post transcriptional mechanisms . ^^^ Furthermore , repression of MyoD dependent transcription by COUP TF 2 occurs in the absence of the nuclear receptor cognate binding motif . ^^^ The inhibition of MyoD mediated trans activation involves the direct binding of the DNA binding domain / C region and hinge / D regions [ i . e . amino acid ( aa ) residues 78 213 ] of COUP TF 2 to the N terminal activation domain of MyoD . ^^^ |
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