| Interacting proteins: P10588 and P24468 |
Pubmed |
SVM Score :0.77142189 |
| Heterodimeric interactions between ARP 1 and Ear 2 may define a distinct pathway of orphan receptor signaling . . 0.77142189^^^ |
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| Interacting proteins: P10588 and P24468 |
Pubmed |
SVM Score :0.0 |
| Transcriptional regulation of human apolipoprotein genes ApoB , ApoCIII , and ApoAII by members of the steroid hormone receptor superfamily HNF 4 , ARP 1 , EAR 2 , and EAR 3 . ^^^ This report shows that four members of the steroid receptor superfamily , ARP 1 , EAR 2 , EAR 3 , and HNF 4 , bind specifically to the regulatory elements BA 1 , CIIIB , and AIIJ . ^^^ Dissociation constant measurements showed that ARP 1 , EAR 2 , and HNF 4 bind to elements BA 1 and CIIIB with similar affinities ( Kd 1 3 nM ) . ^^^ Cotransfection experiments in HepG 2 cells revealed that ARP 1 , EAR 2 , and EAR 3 repressed the BA 1 , CIIIB , and AIIJ element dependent transcription of the reporter gene constructs and the transcription driven by homopolymeric promoters containing either five BA 1 or two CIIIB elements . ^^^ The finding that HNF 4 , ARP 1 , EAR 2 and EAR 3 can regulate the expression of the apoB , apoCIII , and apoAII genes suggest that these nuclear hormone receptors may be an important part of the signal transduction pathways modulating lipid metabolism and cholesterol homeostasis . . ^^^ |
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| Interacting proteins: P10588 and P24468 |
Pubmed |
SVM Score :0.0 |
| Electrophoretic mobility shift analysis of HepG 2 cell nuclear extracts showed that in addition to ARP 1 , site A also binds the orphan nuclear receptors Ear 2 and HNF 4 . ^^^ |
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| Interacting proteins: P10588 and P24468 |
Pubmed |
SVM Score :0.0 |
| We have isolated the murine homologs of the members of the COUP family of steroid hormone receptors , COUP TF 1 , ARP 1 and EAR 2 . ^^^ The expression patterns of COUP TF 1 , ARP 1 and EAR 2 during development were investigated by in situ hybridization . ^^^ |
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| Interacting proteins: P10588 and P24468 |
Pubmed |
SVM Score :0.0 |
| Although HNF 4 , ARP 1 , EAR 2 , and COUP TF bind to the 2C13 promoter in vitro , overexpression of EAR 2 and COUP TF , but not of HNF 4 or ARP 1 , results in the potentiation of the HNF 3 and HNF 1 supported activity in non liver cells . . ^^^ |
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| Interacting proteins: P10588 and P24468 |
Pubmed |
SVM Score :0.0 |
| Using electrophoretic mobility shift assays ( EMSAs ) we show that in vitro translated ARP 1 ( apolipoprotein AI regulatory protein 1 ) , EAR 3 and EAR 2 ( 5 erb A related genes ) bind two RAREs . ^^^ |
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| Interacting proteins: P10588 and P24468 |
Pubmed |
SVM Score :0.0 |
| This element , designated nuclear receptor responsive element ( NRRE ) , spans the 356 to 320 LTR region and contains tightly clustered binding sites for the retinoid 10 receptor alpha ( RXR alpha ) and for five nuclear receptors with unknown ligands , apolipoprotein AI regulatory protein 1 ( ARP 1 ) , 5 erbA related proteins 2 and 3 ( EAR 2 and EAR 3 ) , hepatocyte nuclear factor 4 ( HNF 4 ) , and nerve growth factor inducible protein B ( NGFI B ) . ^^^ The NRRE also interacts with heterodimers formed between RXR alpha and either ARP 1 , EAR 2 , EAR 3 , the retinoic acid receptor alpha ( RAR alpha ) , or the peroxisome proliferator activated receptor ( PPAR ) . ^^^ |
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| Interacting proteins: P10588 and P24468 |
Pubmed |
SVM Score :0.0 |
| Cotransfected orphan receptors COUP TF 1 , ARP 1 , and EAR 2 were able to repress the activity of Oct 4 promoter driven reporters in P 19 EC cells , albeit with different efficiencies . ^^^ |
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| Interacting proteins: P10588 and P24468 |
Pubmed |
SVM Score :0.0 |
| Protein fractionation showed that element J binds at least three hepatic nuclear activities and is also recognized by members of the nuclear receptor family , HNF 4 , EAR 2 , EAR 3 , and ARP 1 . ^^^ |
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| Interacting proteins: P10588 and P24468 |
Pubmed |
SVM Score :0.0 |
| The nuclear orphan receptors COUP TFII and Ear 2 act as silencers of the human oxytocin gene promoter . ^^^ We have previously shown that COUP TFII and Ear 2 , two members of the nuclear orphan receptor family , are able to repress oestrogen stimulated transcriptional activity of the human oxytocin ( OT ) gene promoter by binding to a site that overlaps with the oestrogen response element ( ERE ) present in the 5 ' flanking region of the gene . ^^^ Here we show by co transfection experiments using COUP TFII and Ear 2 expression vectors and reporter constructs containing OT gene promoter fragments linked to the chloramphenicol acetyltransferase gene that both COUP TFII and Ear 2 are capable of silencing basal OT gene promoter activity by 54 and 75 % respectively . 5 ' Deletion and footprint analyses revealed two areas of functionally important interaction sites : ( 1 ) a direct TGACC ( T / C ) repeat overlapping the ERE and ( 2 ) a more promoter proximal area centred at 90 containing three imperfect direct repeats ( R 1 R3 ) spaced by four nucleotides each . ^^^ Moreover , since COUP TFII and Ear 2 are both co expressed in OT expressing uterine epithelial cells , the novel transcriptional effects described here are likely to be of functional importance in the fine tuning of uterine OT gene expression in vivo . . ^^^ |
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| Interacting proteins: P10588 and P24468 |
Pubmed |
SVM Score :0.0 |
| Hepatic enhancement by HCR 1 requires two proximal hormone response elements which have different binding specificities for orphan receptors HNF 4 , ARP 1 , and EAR 2 . ^^^ CIIB is recognized by hepatic nuclear factor 4 ( HNF 4 ) but not ARP 1 or EAR 2 , whereas CIIC is recognized by ARP 1 and EAR 2 but not by HNF 4 . ^^^ A double mutation in elements CIIB and CIIC that eliminated binding of HNF 4 or ARP 1 and EAR 2 , respectively , to these sites abolished the enhancer activity of HCR 1 . ^^^ |
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| Interacting proteins: P10588 and P24468 |
Pubmed |
SVM Score :0.0 |
| Nuclear orphan receptors COUP TFII and Ear 2 : presence in oxytocin producing uterine cells and functional interaction with the oxytocin gene promoter . ^^^ Here we show that the same OT HRE is also capable of interacting with two novel members of the orphan nuclear receptor family , rat COUP TFII and Ear 2 , and that this interaction antagonizes the estrogenic induction of the OT promoter . ^^^ |
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| Interacting proteins: P10588 and P24468 |
Pubmed |
SVM Score :0.0 |
| It was found that element CIIC in addition to ARP 1 and EAR 2 binds RXRalpha / T3Rbeta heterodimers strongly , whereas element CIIB binds hepatic nuclear factor 4 ( HNF 4 ) exclusively . ^^^ |
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| Interacting proteins: P10588 and P24468 |
Pubmed |
SVM Score :0.0 |
| Experiments using DNA binding and competition assays showed that the proximal element B ( 87 / 72 ) binds strongly , in addition to HNF 4 , ARP 1 , EAR 2 , and EAR 3 , heterodimers of RXRalpha with RARalpha , and less efficiently , homodimers of RARalpha and heterodimers of RXRalpha with T3Rbeta or PPARalpha . ^^^ |
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| Interacting proteins: P10588 and P24468 |
Pubmed |
SVM Score :0.0 |
| In vitro translated chicken ovalbumin upstream promoter transcription factor ( COUP TFI ) , ARP 1 , or ErbA related protein 2 ( Ear 2 ) bound both sites . ^^^ |
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| Interacting proteins: P10588 and P24468 |
Pubmed |
SVM Score :0.0 |
| Cloning , expression and regulation of chicken ovalbumin upstream promoter transcription factors ( COUP TFII and EAR 2 ) in the rat anterior pituitary gland . ^^^ To better understand their role in the differentiated adult rat pituitary gland , we cloned COUP TFII and EAR 2 cDNAs from an anterior pituitary cDNA library . ^^^ COUP TFII and EAR 2 pituitary genes display , respectively , 90 and 100 % homologies with their human and mouse homologues . ^^^ Thus , our results have characterized cloning of rat pituitary COUP TFII and EAR 2 genes , demonstrated that they are both specifically expressed in lactotropes , and strongly suggested that they may play an important role in modulating prolactin ( PRL ) gene expression during pregnancy . . ^^^ Chicken ovalbumin upstream promoter transcription factors ( COUP TF ) 2 ( NR2F2 ) and EAR 2 ( NR2F6 ) are structurally related orphan members of the nuclear receptors superfamily . ^^^ |
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