| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| This results in induction and maintained expression of subset specific transcription factors including T bet in Th 1 cells and GATA 3 in Th 2 cells , which are involved in ensuring the commitment of T cells to Th 1 or Th 2 lineages . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| The transcriptional activators T bet and Gata 3 mediate commitment to the T ( H ) 1 and T ( H ) 2 fates , respectively , including chromatin remodeling of signature genes . ^^^ IL 12 and IL 4 also play critical roles in commitment by promoting transcriptional silencing of Gata 3 and T bet , respectively . ^^^ We now show that both T bet and Gata 3 are induced in a cell cycle independent manner in bipotent progenitor cells . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| A number of transcription factors , including GATA 3 and T bet , have been identified that promote the differentiation of T ( H ) 0 cells and the maintenance of the differentiated cell phenotype . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| T Bet expression and failure of GATA 3 cross regulation lead to default production of IFN gamma by gammadelta T cells . gammadelta T cells predominantly produce IFN gamma upon activation . ^^^ To determine the basis for default production of IFN gamma by gammadelta T cells , we analyzed the transcription factors T box expressed in T cells ( T bet ) and GATA 3 . ^^^ Notably , T bet expression after TCR signaling in gammadelta cells was not down regulated by IL 4 , in conjunction with a higher ratio of T bet : GATA 3 expression than that found in CD 4 ( + ) T cells . ^^^ These results indicate that T bet enhances IFN gamma secretion and suppresses IL 4 secretion in gammadelta cells , and that GATA 3 fails to counterbalance T bet mediated IFN gamma production , accounting for the default synthesis of IFN gamma by these T lymphocytes . . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Triggering of LFA 1 on anti CD3 / CD28 stimulated naive Th cells with immobilized Fc ICAM 1 , in the absence of DC and exogenous cytokines , induced a marked shift toward Th 1 cell development , accompanied by a dose dependent decrease in GATA 3 expression and a dose dependent increase in T bet expression . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| During primary antigenic stimulation of naive Th 0 cells from DO11 . 10 mice , AGN 194204 increased IL 4 and IL 5 production , decreased IFN gamma production , increased mRNA in responding T cells for genes involved in Th 2 development ( IL 4 , GATA 3 , and c maf ) , and decreased mRNA for genes involved in Th 1 development ( IFN gamma , T bet , and IL 12R ) . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| The binding of transcription factors GATA 3 or T bet to specific enhancers does recruit transcription factors such as NFAT 1 to IL 4 or IFNgamma promoters , respectively ; however , GATA 3 also intrinsically suppresses T bet and vice versa . ^^^ Silencing of GATA 3 / T bet , which is influenced by factors such as cytokines , is associated with irreversible Th polarization . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Meanwhile , the change of IFN gamma , IL 4 , T bet and GATA 3 in PBMC before and after being cultured with the traditional Chinese herbal medicine Astragulus and Tetramethylpyrazine was also observed . ^^^ But , the positive rates of transcription factor T bet and GATA 3 were 16 / 42 and 34 / 42 . ^^^ CONCLUSION : Predominant expression of Th 2 type cytokines may be related to lower expression of T bet or higher expression of GATA 3 . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Ectopic T bet expression selectively induced expression of IL 12Rbeta2 , but not IL 18Ralpha , in wild type and STAT 1 ( / ) T ( H ) 2 cells , but did not extinguish expression of GATA 3 and T ( H ) 2 cytokines . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| This step modulates the committed precursor frequency of T cell subsets and influences the expression of T box expressed in T cells ( T bet ) and GATA 3 genes . ^^^ The second step requires antigenic stimulation of T cells together with IL 12 or IL 4 , and influences on the expression of T bet and GATA 3 . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| These changes in histone acetylation status are locus and lineage specific , and are maintained by the transcription factors Tbet and GATA 3 in a STAT dependent manner . ^^^ In this model , GATA 3 and Tbet serve as transcriptional maintenance factors , which keep the locus accessible to the transcriptional machinery . . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Furthermore , we have examined the levels of GATA 3 , c Maf , T bet , and Ets related molecule during human Th1 / Th2 differentiation and suggest that differences in the levels of these critical transcription factors are responsible for commitment toward the Th 1 or Th 2 lineage . . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Furthermore , expression of Th 1 and Th 2 specific transcription factors T bet and GATA 3 , as well as the nuclear factor of activated T cells transcription factor , NFATc , was not significantly different between the two mice . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| The identification of transcription factors controlling T ( H ) 1 and T ( H ) 2 development further support the T ( H ) 2 hypothesis because GATA 3 is overexpressed and T bet is underexpressed in the asthmatic airway . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| GATA 3 suppresses Th 1 development by downregulation of Stat 4 and not through effects on IL 12Rbeta2 chain or T bet . ^^^ To further understand the interaction among GATA 3 , Stat 4 , and T bet in helper T cell development , we first showed that retroviral expression of GATA 3 in developing Th 1 cells suppresses Th 1 development through downregulation of Stat 4 rather through downregulation of the IL 12Rbeta2 chain . ^^^ Then , using cells doubly infected with GFP and YFP expressing retroviruses , we showed that retroviral GATA 3 expression in developing Th 1 cells does not block Th 1 development in cells coexpressing Stat 4 but does so in cells coexpressing T bet . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| At week 14 postinfection ( mid acute phase ) , mRNAs for leukocyte markers ( CD 3 , CD 4 , CD 8 ) , type 1 cytokines and related transcription factors ( IFN gamma , TNF alpha , STAT 4 , T bet ) , and IL 10 were increased in livers from resolving infections , but mRNAs of other type 1 ( IL 2 ) and type 2 ( IL 4 , STAT 6 , and GATA 3 markers remained at baseline levels . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Glucocorticoids inhibit the induction of GATA 3 and T bet transcription factors . ( 4 ) The influence of ER / Smad 4 signaling cross communication on prolactinoma pathogenesis . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| This process ensures that cytokine genes remain accessible to the relevant transcription factors and promotes functional cooperation of the inducible transcription factor NFAT with lineage specific transcription factors such as GATA 3 and T bet . . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| GATA 3 for Th 2 and T bet for Th 1 cells expressing interleukin 4 or interferon gamma , respectively , are prime candidates for key transcription factors of cytokine memory . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| TGF beta has been shown to inhibit the expression of transcription factors such as GATA 3 and T bet that play important roles in T cell differentiation . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Both positive percentage and relative intensity were lower in transcript factor for Th 1 cytokine , T bet ( 40 . 5 % and 0 . 139 , respectively ) than those for Th 2 cytokine , GATA 3 ( 89 . 2 % and 0 . 364 , respectively ) . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| T bet / GATA 3 ratio as a measure of the Th1 / Th2 cytokine profile in mixed cell populations : predominant role of GATA 3 . ^^^ The differentiation of naive T helper ( Th ) cells towards Th 1 or Th 2 cells is regulated by the transcription factors T box expressed in T cells ( T bet ) and GATA binding protein 3 ( GATA 3 ) . ^^^ In the present study , the gene expression of T bet and GATA 3 was measured by semi quantitive reverse transcription polymerase chain reaction ( RT PCR ) in Th 1 and Th 2 cells derived from purified splenic CD4+ T cells from DO11 . 10 / Rag2 ( / ) transgenic mice and control BioBreeding ( BBc ) Wistar rat splenic T cells stimulated under Th 1 or Th 2 conditions . ^^^ In both sets of experiments , changes in the ratio of expression of T bet and GATA 3 reflected changes in the Th 1 specific cytokine interferon gamma ( IFN gamma ) and Th 2 specific cytokine interleukin ( IL ) 4 . ^^^ T bet gene expression was not maintained in fully polarized rat Th 1 cells whereas GATA 3 gene expression was maintained in long term polarized rat Th 2 cells , indicating that maintenance of Th1 / Th2 status occurred more as a result of altered GATA 3 mRNA expression than T bet . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Different antigens trigger different Th1 / Th2 reactions in neonatal mononuclear cells ( MNCs ) relating to T bet / GATA 3 expression . ^^^ Employing Dermatophagoides pteronyssinus ( Der p ) extract and varicella zoster virus ( VZV ) as antigens , we assessed Th1 / Th2 reactions as demonstrated by IL 4 / IFNgamma production and mRNA expression , and transcriptional factors T bet / GATA 3 mRNA expression in mononuclear cells from human umbilical cord blood ( CBMC ) . ^^^ VZV enhanced Th 1 related transcription factor T bet mRNA expression , in association with later down regulation of Th 2 related GATA 3 mRNA expression in PBMC . ^^^ However , VZV did not up regulate T bet or down regulate GATA 3 expression significantly in CBMC . ^^^ In contrast , Der p induced an early GATA 3 expression and later T bet expression in CBMC . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Here , we tested the flexibility of human Th 1 or Th 2 differentiation by forced expression of transcription factors T bet and GATA 3 . ^^^ Ectopic expression of T bet and GATA 3 in freshly isolated human T ( N ) cells resulted in their differentiation to a Th 1 and Th 2 phenotype , respectively , in the absence of polarizing cytokines . ^^^ Interestingly , similar to the naive T cell subset , central memory T cells were also largely programmed toward Th 1 or Th 2 effector cells upon expression of T bet and GATA 3 , respectively . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Stat 1 is required for suppression of GATA 3 , but T bet induction by IL 27 can also be mediated through a Stat 1 independent pathway . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| The present study analyzed the expression of the following 13 TFs : AML 1 , CEBPA , E2A , ETS 1 , GATA 1 , GATA 2 , GATA 3 , IKAROS , IRF 1 , PAX 5 , PU 1 , TBET and TCF 1 in 7 malignant NK cell lines together with 5 malignant NKT cell lines , 5 T cell acute lymphoblastic leukemia ( ALL ) cell lines including 3 gamma / delta T cell receptor ( TCR ) type and 2 alpha / beta TCR type , and 3 B cell precursor ( BCP ) leukemia cell lines . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Combining cytokine signalling with T bet and GATA 3 regulation in Th 1 and Th 2 differentiation : a model for cellular decision making . ^^^ Differentiation of uncommitted T cells into Th 1 and Th 2 subpopulations depends on both intracellular events controlling expression of transcription factors T bet and GATA 3 and interactions between cells mediated by cytokines , particularly IL 4 and IFNgamma . ^^^ It shows how antigen stimulation in conjunction with cytokines and other extracellular signals gives rise to rapid , reversible and mutually exclusive expression of T bet or GATA 3 due to feedback between the transcription factors and their signalling pathways . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Further , it stabilized IL 4 production and GATA 3 expression in differentiated Th 2 cells , whereas in differentiated Th 1 cells it inhibited the expression of T bet and reduced the production of IFN gamma . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Chronic morphine treatment differentiates T helper cells to Th 2 effector cells by modulating transcription factors GATA 3 and T bet . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| To clarify the functional state of T cells in Kawasaki disease , we analysed mRNA expression levels of Th1 / Th2 cytokines ( IFN gamma and IL 4 ) along with Th1 / Th2 inducing transcription factors , T bet and GATA 3 , which play pivotal roles in the development of Th 1 and Th 2 cells , respectively . ^^^ These results suggest that the suppressed function of Th 1 and Th 2 , associated with the suppression of both T bet and GATA 3 gene expression , may be one of the immunological characteristics of Kawasaki disease . . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Effect of treatment of rheumatoid arthritis with infliximab on IFN gamma , IL 4 , T bet , and GATA 3 expression : link with improvement of systemic inflammation and disease activity . ^^^ OBJECTIVE : To study interferon gamma ( IFN gamma ) production and the expression of T bet and GATA 3 , the transcription factors associated with Th 1 and Th 2 , in peripheral blood mononuclear cells ( PBMC ) from patients with rheumatoid arthritis before and during infliximab treatment , so as to distinguish between a disease specific and a disease activity dependent defect . ^^^ IFN gamma concentrations in supernatants were determined by ELISA . mRNA expression of IFN gamma , IL 4 , T bet , and GATA 3 was determined by real time RT PCR in whole blood at weeks 0 and 22 . ^^^ T bet expression or T bet / GATA 3 ratio of rheumatoid blood was less than in controls . ^^^ The T bet / GATA 3 ratio was not influenced by infliximab treatment . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| T helper cell fate specified by kinase mediated interaction of T bet with GATA 3 . ^^^ Cell lineage specification depends on both gene activation and gene silencing , and in the differentiation of T helper progenitors to Th 1 or Th 2 effector cells , this requires the action of two opposing transcription factors , T bet and GATA 3 . ^^^ T bet is essential for the development of Th 1 cells , and GATA 3 performs an equivalent role in Th 2 development . ^^^ We report that T bet represses Th 2 lineage commitment through tyrosine kinase mediated interaction between the two transcription factors that interferes with the binding of GATA 3 to its target DNA . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| We now report that HSP 60 in vitro differentially modulates the expression of Th1 / Th2 transcription factors in human T cells : HSP 60 down regulates T bet , NF kappaB , and NFATp and up regulates GATA 3 , leading to decreased secretion of TNF alpha and IFN gamma and enhanced secretion of IL 10 . ^^^ In BALB / c mice , HSP 60 in vivo inhibited the clinical , histological , and serological manifestations of Con A induced hepatitis associated with up regulated T cell expression of suppressor of cytokine signaling 3 and GATA 3 and down regulated T bet expression . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Effects of ginsenoside F 3 on mRNA level of cytokines IL 4 , IFN gamma , and transcription factors T bet and GATA 3 were evaluated by RT PCR analysis . ^^^ RT PCR analysis displayed that ginsenoside F 3 enhanced the IFN gamma and T bet gene expression and decreased IL 4 and GATA 3 gene expression . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| It has been shown that cytokine signaling molecules , including transcription factors T bet and GATA 3 , interleukin 12 receptor beta 2 ( IL 12R beta 2 ) and suppressors of cytokine signaling ( SOCS ) , such as SOCS 1 , are important in differentiation of naive T cells into Th 1 helper T cells . ^^^ Expression of T bet , GATA 3 , IL 12R beta 2 and SOCS 1 was significantly increased in HAM / TSP patients in comparison with HTLV 1 uninfected individuals . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| At the onset of shock , compared to healthy volunteers , mRNA levels in septic shock patients were increased for IL 10 , IL 1beta , and high mobility group B 1 ( HMGB 1 ) and decreased for transforming growth factor beta 1 , the Th 1 , and Th 2 transcription factors , T bet and GATA 3 , respectively . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Expression of the T cell transcription factors , GATA 3 and T bet , in the neoplastic cells of Hodgkin lymphomas . ^^^ Out of HL cell lines , T bet was expressed in five , GATA 3 in four , and c Maf in two cell lines . ^^^ Immunohistochemistry in HL tissues revealed that in 11 of 12 ( 92 % ) of the classical HL cases HRS cells were GATA 3 and / or T bet positive . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Nevertheless , after culture in either Th 1 polarizing or neutral conditions , mRNA was present for both T bet and GATA 3 as well as for IL 12Rbeta2 in WC1 . 1+ and WC1 . 2+ subpopulations , and neither produced IL 4 under any conditions . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| These effects were associated with suppression of OVA specific IL 4 production and GATA 3 expression and with up regulation of IFN gamma production and T bet expression by splenocytes . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| We report that Vav 1 / CD4+ T cells display deficient T cell receptor ( TCR ) / CD28 induced IL 4 and c Maf expression and , conversely , enhanced interferon gamma ( IFN gamma ) production and T bet expression ( even when cultured under Th 2 polarizing conditions ) , but intact expression of other Th 2 cytokines and GATA 3 . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| T bet ( T box expressed in T cells ) and GATA 3 are transcription factors that play a critical role in the development of Th 1 and Th 2 cells , as do genes of the SOCS ( suppressor of cytokine signaling ) family , albeit indirectly . ^^^ T bet ( but not GATA 3 ) was expressed at significantly lower levels in cells of filaria infected individuals in response to BmA compared with those from the uninfected group , accounting , at least partially , for the diminished IFN gamma expression . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| To investigate the roles of TFs in human B cell precursors ( BCPs ) , the present study analyzed the expression of the following 16 hematopoietic TFs : AML 1 , C / EBPalpha , C / EBPbeta , C / EBPgamma , C / EBPepsilon , E2A , Ets 1 , GATA 1 , GATA 2 , GATA 3 , Ikaros , IRF 1 , Pax 5 , PU . 1 , T bet and TCF 1 in 30 human BCP leukemia cell lines . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Treatment of PLP ( 139 151 ) specific T cells in vitro with AICAR decreased their expression of T bet in response to IL 12 , a Th 1 transcription factor , whereas in response to IL 4 , it induced the expression and phosphorylation of Th 2 transcription factors GATA 3 and STAT 6 , respectively . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| RNA quantification of T bet , GATA 3 , IFN gamma , and IL 4 shows a defective Th 1 immune response in the peripheral blood from rheumatoid arthritis patients : link with disease activity . ^^^ T bet and GATA 3 transcription factors associated with Th 1 and Th 2 responses respectively , and IFNgamma and IL 4 mRNA levels were measured by real time PCR . ^^^ In comparison to RA patients with a low CRP ( CRP < 40 mg / l ) , patients with a high CRP ( CRP > or=40 mg / l ) had lower IFNgamma / beta actin , T bet / beta actin mRNA levels and T bet / GATA 3 expression ratios . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| To investigate whether dengue 2 patients with and without dengue hemorrhagic fever had different virus load , immune mediators , or T helper ( Th ) reaction , we simultaneously measured virus load , immune mediators and the Th1 / Th2 transcription factors T bet / GATA 3 mRNA expression in a large outbreak of dengue 2 infections in Southern Taiwan . ^^^ Further studies showed that patients with dengue hemorrhagic fever had a significantly lower T bet than those with dengue fever , but GATA 3 mRNA expression in peripheral blood leukocytes was not significant difference between both groups . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| T cells primed with TGF beta secreted IFN gamma in response to both T cell receptor ligation and IL 12 / IL 18 stimulation , and expressed high levels of T bet and low levels of GATA 3 . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Measurements of the transcriptional regulators of T cell polarization revealed that MMP 12 null cells had increased T bet and reduced GATA 3 expression , a condition that favors a Th 1 bias . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| However , they were comparable with the IFN gamma+ or IL 4+ cells in the expression levels of other Th1 / Th2 marker genes ( GATA 3 , Tbet , and IL12Rbeta2 ) . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| To further explore longitudinal changes in peritoneal immunity during PD related peritonitis , we examined the production of interleukin 12 ( IL 12 ) , IL 18 , and interferon gamma ( IFN gamma ) in peritoneal dialysate effluent ( PDE ) and kinetic expression of the transcription factors T box expressed in T cells ( T bet ) and guanine adenine thymine adenine ( GATA ) binding protein 3 ( GATA 3 ) in peritoneal T cells during peritonitis . ^^^ METHODS : IL 12 , IL 18 , and IFN gamma protein and IFN gamma , T bet , and GATA 3 messenger RNA ( mRNA ) were measured in PDE during various phases of peritonitis in 40 patients undergoing PD . ^^^ The kinetics of IFN gamma , T bet , and GATA 3 mRNA expression in peritoneal T cells , measured by means of real time polymerase chain reaction , differed between the 2 groups . ^^^ In the rapid response group , IFN gamma and T bet mRNA expression increased , whereas that of GATA 3 decreased over time . ^^^ Results were opposite in the delayed response group , with IFN gamma and T bet levels decreasing and GATA 3 levels increasing over time . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Of note , IFN alpha enhanced the expression of mRNA for c Maf , T bet , and Fox P 3 , irrespective of the presence of IL 12 , but not that for GATA 3 , in anti CD 3 stimulated CD4+ T cells . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| DESIGN AND METHODS : The balance of Th1 / Th2 and Tc1 / Tc2 was studied by simultaneous analysis of intracellular cytokines of peripheral blood mononuclear cells and splenocytes in short term cultures activated with PMA / ionomycin as well as mRNA expression of T bet and GATA 3 in peripheral blood mononuclear cells and splenocytes using real time polymerase chain reaction . ^^^ GATA 3 mRNA expression in ITP patients was significantly lower both in PB ( p < 0 . 01 ) and in splenocytes ( p < 0 . 01 ) than in corresponding samples from controls while there was no difference in T bet expression . ^^^ The T bet / GATA 3 ratio may provide a surrogate marker of T1 / T2 cytokine balance . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| RESULTS : The expression ratio of T bet : Gata 3 , master transcription factors for Th 1 and Th 2 cytokines , was increased in the MLN from NTP 2000 fed BBdp rats compared with that from BBc rats , mainly due to decreased Gata 3 expression . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Meanwhile , both positive percentage and relative intensity of gene expression were lower for a type 1 cytokine related transcription factor T bet ( 31 . 4 % and 0 . 142 , respectively ) than those for type 2 cytokine related GATA 3 ( 85 . 7 % and 0 . 378 , respectively ) . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Similarly to the key regulators , TBX 21 and GATA 3 , the transcription factors SATB 1 , TCF 7 and BCL 6 were differentially regulated at the protein level during early Th 1 and Th 2 cell polarization . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Effects of CpG oligodeoxynucleotide on transcription factors GATA 3 and T bet mRNA expression in asthmatic mice . ^^^ AIM : To investigate effects of CpG oligodeoxynucleotide ( CpG ODN ) on the mRNA expression of transcription factors GATA binding protein 3 ( GATA 3 ) and T box expressed in T cells ( T bet ) in asthmatic mice . ^^^ Transcription factor GATA 3 and T bet mRNA expression in splenocytes and lung tissue were detected by reverse transcription polymerase chain reaction . ^^^ GATA 3 mRNA expression was reduced in both lung tissue and splenocytes in the CpG ODN treated group , while the mRNA ratio of T bet to GATA 3 in splenocytes was increased . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Despite the difference in IFN gamma production , cells bearing either WC 1 isoform had similar transcription levels of the high affinity IL 12 receptor subunit ( IL 12Rbeta2 ) as well as of the transcription factors T bet and GATA 3 when cultured with IL 12 . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| The effects of allitridin on the expression of transcription factors T bet and GATA 3 in mice infected by murine cytomegalovirus . ^^^ This study was designed to investigate the effects of allitridin on the expression of transcription factors T bet and GATA 3 in mice infected by murine cytomegalovirus ( MCMV ) . ^^^ The expression levels of the transcription factors T bet and GATA 3 were measured by reverse transcription polymerase chain reaction . ^^^ MCMV infection markedly down modulated the expression of IFN gamma and T bet and significantly up modulated the expression of IL 10 and GATA 3 . ^^^ Allitridin induced significantly ( P < . 01 ) increased expression of the transcription factor T bet and the Th 1 cytokine IFN gamma and markedly ( P < . 01 ) decreased expression of the transcription factor GATA 3 and the Th 2 cytokine IL 10 . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Up regulation correlated with increased expression of GATA 3 and signal transducer and activator of transcription 6 ( Stat 6 ) , Th 2 specific transcription factors and decreased expression of T bet , a transcription factor involved in Th 1 differentiation . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| In contrast to GATA 3 , NFAT , and NF kappaB , intrapair variability of T bet , the master transcription factor of Th 1 cells , was very low among monozygotic and high among dizygotic twins , indicative of a strong genetic influence on T bet ( heritability 0 . 93 , 95 % confidence interval , 0 . 84 1 . 0 ) . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Second , using artificial antigen presenting cells to specifically isolate self reactive T cells , we demonstrate that ASCT induces autoimmune cells to deviate from a proinflammatory phenotype ( mRNA interferon gamma [ IFN gamma ] and T bet high ) to a tolerant phenotype ( mRNA interleukin 10 [ IL 10 ] and GATA 3 high ) . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Consistent with this observation , anti GITR antibody significantly enhanced the expression of the key Th 1 ( T bet ) and Th 2 ( GATA 3 ) transcription factors in vitro . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Here , we studied T bet expression kinetics in parallel with GATA 3 during Th1 / Th2 polarization . ^^^ In addition , we show that the immunosuppressive cytokine TGF beta inhibits T bet and GATA 3 protein expression only if it is present prior to primary T cell activation and is maintained in the cultures during the early polarization of Th1 / Th2 cells . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| LLO treatment resulted in a reduction of GATA 3 and SOCS 3 expressions but induced the transcription of T bet instead . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Helper T cells from methyl CpG binding domain protein 2 deficient mice exhibit exuberant patterns of cytokine expression despite appropriate silencing of genes encoding the lineage specifying factors T bet and GATA 3 . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Using conditional introduction of dominant negative factors , we now show that T bet and GATA 3 are far more critical in establishment than maintenance of IFN gamma and IL 4 activity during Th 1 and Th 2 maturation , respectively . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Overexpression of the transcription factor T bet in the absence of significant GATA 3 expression further assessed the type 1 polarization of aortic wall immune responses . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Interestingly , the modified plasmid vaccine predominantly enhanced the type 2 immune responses manifested by an increased IgG 1 to IgG2a antibody ratio and a greater induction of GATA 3 and IL 4 mRNA than that of T bet and IFN gamma mRNA in spleen cells from vaccinated mice . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| The identification of transcription factors controlling Th 1 , Th 2 and T regulatory cell ( T ( Reg ) ) development further support the Th 2 hypothesis since GATA 3 is overexpressed and T bet underexpressed in the asthmatic airway and Foxp 3 is induced in asthma by corticosteroid treatment . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| This network encompasses 17 nodes , namely IFN gamma , IL 4 , IL 12 , IL 18 , IFN beta , IFN gammaR , IL 4R , IL 12R , IL 18R , IFN betaR , STAT 1 , STAT 6 , STAT 4 , IRAK , SOCS 1 , GATA 3 , and T bet , as well as their cross regulatory interactions . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| The levels of epsilon germline transcription and the amounts of mRNA for IL 4 , IFN gamma , GATA 3 and T bet gene in the spleen were evaluated by real time RT PCR at the end of the experiment . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Since T bet and GATA 3 are the principal transcription factors for the differentiation of type 1 and type 2 helper T lymphocytes , respectively , we studied their mRNA expression in the urinary sediment of SLE patients and compared this with their urinary and intra renal protein expression . ^^^ Urinary mRNA expression of T bet and GATA 3 were studied by the real time quantitative polymerase chain reaction . ^^^ Intra renal and urinary expressions of T bet and GATA 3 were studied by immunohistochemistry and western blotting , respectively . ^^^ For the 22 SLE patients with kidney biopsy , tubular expressions of T bet and GATA 3 significantly correlated with the histological activity index ( T bet : r = 0 . 57 , P = 0 . 006 ; GATA 3 : r = 0 . 79 , P < 0 . 001 ) . ^^^ CONCLUSIONS : Patients with active lupus nephritis have increased T bet and depressed GATA 3 expression in the urinary sediment and kidney tissue , indicating a predominant Th 1 type of T lymphocyte activation . . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Transcription factors GATA 3 and T bet , as well as its target Hlx , are absent in IL 17 producing T cells , and they do not express the negative regulator for TGFbeta signaling , Smad 7 . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Cytotoxic T lymphocyte antigen 4 inhibits GATA 3 but not T bet mRNA expression during T helper cell differentiation . ^^^ Naive CD4+ T cell differentiation to T helper 1 ( Th 1 ) and Th 2 cells is dependent on T bet and GATA 3 factors , respectively . ^^^ T bet and GATA 3 , indeed , through chromatin remodelling allow transcriptional activation of Ifngamma and Th 2 cytokine ( Il 4 , Il 5 , Il 13 ) genes , respectively . ^^^ We investigated the effects of the negative costimulatory receptor cytotoxic T lymphocyte antigen 4 ( CTLA 4 ) on GATA 3 and T bet mRNA expression and Th cell differentiation in mouse naive CD4+ T cells . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| First , impaired induction of T bet and GATA 3 mRNA underlies the Th1 / Th2 deficiency in infected individuals . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Regulation of GATA 3 , c maf and T bet mRNA expression in bronchoalveolar lavage cells and bronchial biopsies after segmental allergen challenge . ^^^ BACKGROUND : GATA 3 ( GATA binding protein 3 ) and the proto oncogene c maf are Th 2 regulating transcription factors that control the expression of interleukin ( IL ) 4 and IL 5 , while T bet ( T box expressed in T cells ) is a Th 1 specific transcription factor that controls the expression of interferon ( IFN gamma ) . ^^^ METHODS : The mRNA expression of GATA 3 , c maf and T bet was determined by real time polymerase chain reaction in bronchoalveolar lavage ( BAL ) cells and bronchial biopsies . ^^^ CONCLUSIONS : Segmental allergen challenge in asthmatics leads to increased GATA 3 , c maf and T bet expression in BAL cells but not in bronchial biopsies . . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| In contrast , mRNAs encoding T cell transcription factors T bet and GATA 3 , associated with differentiation of Th 1 and Th 2 CD4+ T cells respectively , were unchanged . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Analyses of each intermediate stage for CD 34 , CD 117 , and CD 94 cell surface expression , lineage differentiation potentials , capacity for cytokine production and natural cytotoxicity , and ETS 1 , GATA 3 , and T BET expression provide evidence for a new model of human NK cell differentiation in secondary lymphoid tissues . . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Transcription factors T bet and GATA 3 regulate development of airway remodeling . ^^^ Although asthma is considered to be a Th 2 disease , the role of T bet and GATA 3 , the key transcription factors for differentiation toward Th 1 and Th 2 cells , in the pathogenesis of airway remodeling is poorly understood . ^^^ OBJECTIVES : We therefore examined the effects of GATA 3 or T bet induction of Th1 / Th2 bias on the development of airway remodeling in mice . ^^^ METHODS : The development of airway remodeling after repeated allergen challenges was analyzed using transgenic mice overexpressing either GATA 3 or T bet . ^^^ Eosinophilic airway inflammation increased in mice overexpressing GATA 3 , but decreased in mice overexpressing T bet after repeated allergen exposure . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| T bet regulates Th 1 responses through essential effects on GATA 3 function rather than on IFNG gene acetylation and transcription . ^^^ T helper type 1 ( Th 1 ) development is facilitated by interrelated changes in key intracellular factors , particularly signal transducer and activator of transcription ( STAT ) 4 , T bet , and GATA 3 . ^^^ Here we show that CD4+ cells from T bet / mice are skewed toward Th 2 differentiation by high endogenous GATA 3 levels but exhibit virtually normal Th 1 differentiation provided that GATA 3 levels are regulated at an early stage by anti interleukin ( IL ) 4 blockade of IL 4 receptor ( R ) signaling . ^^^ In related studies , we show that the negative effect of GATA 3 on Th 1 differentiation in T bet / cells arises from its ability to suppress STAT 4 levels , because if this is prevented by a STAT 4 expressing retrovirus , normal Th 1 differentiation is observed . ^^^ Finally , we show that retroviral T bet expression in developing and established Th 2 cells leads to down regulation of GATA 3 levels . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Peripheral blood mononuclear cells ( PBMCs ) were isolated and cultured with CVB 4 and analyzed for cytokine and chemokine receptors by flow cytometry and for expression of transcription factors Tbet and GATA 3 by RT PCR and Western blot . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Treatment of allergic airway inflammation and hyperresponsiveness by imiquimod modulating transcription factors T bet and GATA 3 . ^^^ Several investigators have shown that T bet and GATA 3 are master Th 1 and Th 2 regulatory transcription factors . ^^^ This study investigated whether imiquimod treatment inhibited airway inflammation by modulating transcription factors T bet and GATA 3 . ^^^ The mRNA expressions of IL 4 , IL 5 , IFN gamma , T bet and GATA 3 in lung and in CD 4 ( + ) T cells were determined by reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ The protein expressions of T bet and GATA 3 were measured by Western blot . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| These simvastatin conditioned DCs up regulated GATA 3 expression and down regulated T bet expression in cocultured CD4+ T cells in the absence of additional simvastatin added to the coculture . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| We then measured serum urea levels , performed histomorphometric analysis for tubular necrosis and regeneration , and evaluated the mRNA expression of HO 1 , t bet , Gata 3 and MCP 1 by real time PCR at 24 , 48 and 120 h after surgery . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| T bet , regulator of the Th 1 response , was up regulated in CDD fed mice , whereas Th 2 regulator GATA 3 was significantly suppressed in CDD fed mice after ConA . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| METHODS : Levels of transcripts encoding for pro and anti inflammatory markers ( tnf alpha , ifn gamma , il 10 , t bet , gata 3 ) as well as for TGF beta 1 signaling mediators ( smad 3 , smad 4 , smad 7 ) were followed by real time PCR in a prospective study enrolling 6 AGMs and 6 MACs . ^^^ In AGMs , we found increases of gata 3 but not t bet , a longer lasting up regulation of smad 4 ( days 1 21 p . i ) , a mediator enhancing TGF beta 1 signaling , and no smad 7 up regulations . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| Likewise , analysis of transcription factors shows increased T bet with respect to both allergens , but also GATA 3 with respect to Ber e 1 . ^^^ |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| RESULTS : We developed real time PCR based detection assays for several immune related transcription factor and cytokine genes from deer mice that permit the profiling of CD4+ helper T cells , including markers of Th 1 cells ( T bet , STAT 4 , IFNgamma , TNF , LT ) , Th 2 cells ( GATA 3 , STAT 6 , IL 4 , IL 5 ) and regulatory T cells ( Fox p 3 , IL 10 , TGFbeta 1 ) . ^^^ |
|
| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23771 and Q9UL17 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Triggering of LFA 1 on anti CD3 / CD28 stimulated naive Th cells with immobilized Fc ICAM 1 , in the absence of DC and exogenous cytokines , induced a marked shift toward Th 1 cell development , accompanied by a dose dependent decrease in GATA 3 expression and a dose dependent increase in T bet expression . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Similarly to the key regulators , TBX 21 and GATA 3 , the transcription factors SATB 1 , TCF 7 and BCL 6 were differentially regulated at the protein level during early Th 1 and Th 2 cell polarization . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| This results in induction and maintained expression of subset specific transcription factors including T bet in Th 1 cells and GATA 3 in Th 2 cells , which are involved in ensuring the commitment of T cells to Th 1 or Th 2 lineages . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| The transcriptional activators T bet and Gata 3 mediate commitment to the T ( H ) 1 and T ( H ) 2 fates , respectively , including chromatin remodeling of signature genes . ^^^ IL 12 and IL 4 also play critical roles in commitment by promoting transcriptional silencing of Gata 3 and T bet , respectively . ^^^ We now show that both T bet and Gata 3 are induced in a cell cycle independent manner in bipotent progenitor cells . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| A number of transcription factors , including GATA 3 and T bet , have been identified that promote the differentiation of T ( H ) 0 cells and the maintenance of the differentiated cell phenotype . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| T Bet expression and failure of GATA 3 cross regulation lead to default production of IFN gamma by gammadelta T cells . gammadelta T cells predominantly produce IFN gamma upon activation . ^^^ To determine the basis for default production of IFN gamma by gammadelta T cells , we analyzed the transcription factors T box expressed in T cells ( T bet ) and GATA 3 . ^^^ Notably , T bet expression after TCR signaling in gammadelta cells was not down regulated by IL 4 , in conjunction with a higher ratio of T bet : GATA 3 expression than that found in CD 4 ( + ) T cells . ^^^ These results indicate that T bet enhances IFN gamma secretion and suppresses IL 4 secretion in gammadelta cells , and that GATA 3 fails to counterbalance T bet mediated IFN gamma production , accounting for the default synthesis of IFN gamma by these T lymphocytes . . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| During primary antigenic stimulation of naive Th 0 cells from DO11 . 10 mice , AGN 194204 increased IL 4 and IL 5 production , decreased IFN gamma production , increased mRNA in responding T cells for genes involved in Th 2 development ( IL 4 , GATA 3 , and c maf ) , and decreased mRNA for genes involved in Th 1 development ( IFN gamma , T bet , and IL 12R ) . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| The binding of transcription factors GATA 3 or T bet to specific enhancers does recruit transcription factors such as NFAT 1 to IL 4 or IFNgamma promoters , respectively ; however , GATA 3 also intrinsically suppresses T bet and vice versa . ^^^ Silencing of GATA 3 / T bet , which is influenced by factors such as cytokines , is associated with irreversible Th polarization . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Meanwhile , the change of IFN gamma , IL 4 , T bet and GATA 3 in PBMC before and after being cultured with the traditional Chinese herbal medicine Astragulus and Tetramethylpyrazine was also observed . ^^^ But , the positive rates of transcription factor T bet and GATA 3 were 16 / 42 and 34 / 42 . ^^^ CONCLUSION : Predominant expression of Th 2 type cytokines may be related to lower expression of T bet or higher expression of GATA 3 . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Ectopic T bet expression selectively induced expression of IL 12Rbeta2 , but not IL 18Ralpha , in wild type and STAT 1 ( / ) T ( H ) 2 cells , but did not extinguish expression of GATA 3 and T ( H ) 2 cytokines . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| This step modulates the committed precursor frequency of T cell subsets and influences the expression of T box expressed in T cells ( T bet ) and GATA 3 genes . ^^^ The second step requires antigenic stimulation of T cells together with IL 12 or IL 4 , and influences on the expression of T bet and GATA 3 . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| This process ensures that cytokine genes remain accessible to the relevant transcription factors and promotes functional cooperation of the inducible transcription factor NFAT with lineage specific transcription factors such as GATA 3 and T bet . . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| GATA 3 for Th 2 and T bet for Th 1 cells expressing interleukin 4 or interferon gamma , respectively , are prime candidates for key transcription factors of cytokine memory . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| These changes in histone acetylation status are locus and lineage specific , and are maintained by the transcription factors Tbet and GATA 3 in a STAT dependent manner . ^^^ In this model , GATA 3 and Tbet serve as transcriptional maintenance factors , which keep the locus accessible to the transcriptional machinery . . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Furthermore , we have examined the levels of GATA 3 , c Maf , T bet , and Ets related molecule during human Th1 / Th2 differentiation and suggest that differences in the levels of these critical transcription factors are responsible for commitment toward the Th 1 or Th 2 lineage . . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Furthermore , expression of Th 1 and Th 2 specific transcription factors T bet and GATA 3 , as well as the nuclear factor of activated T cells transcription factor , NFATc , was not significantly different between the two mice . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| The identification of transcription factors controlling T ( H ) 1 and T ( H ) 2 development further support the T ( H ) 2 hypothesis because GATA 3 is overexpressed and T bet is underexpressed in the asthmatic airway . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| GATA 3 suppresses Th 1 development by downregulation of Stat 4 and not through effects on IL 12Rbeta2 chain or T bet . ^^^ To further understand the interaction among GATA 3 , Stat 4 , and T bet in helper T cell development , we first showed that retroviral expression of GATA 3 in developing Th 1 cells suppresses Th 1 development through downregulation of Stat 4 rather through downregulation of the IL 12Rbeta2 chain . ^^^ Then , using cells doubly infected with GFP and YFP expressing retroviruses , we showed that retroviral GATA 3 expression in developing Th 1 cells does not block Th 1 development in cells coexpressing Stat 4 but does so in cells coexpressing T bet . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| At week 14 postinfection ( mid acute phase ) , mRNAs for leukocyte markers ( CD 3 , CD 4 , CD 8 ) , type 1 cytokines and related transcription factors ( IFN gamma , TNF alpha , STAT 4 , T bet ) , and IL 10 were increased in livers from resolving infections , but mRNAs of other type 1 ( IL 2 ) and type 2 ( IL 4 , STAT 6 , and GATA 3 markers remained at baseline levels . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Glucocorticoids inhibit the induction of GATA 3 and T bet transcription factors . ( 4 ) The influence of ER / Smad 4 signaling cross communication on prolactinoma pathogenesis . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| TGF beta has been shown to inhibit the expression of transcription factors such as GATA 3 and T bet that play important roles in T cell differentiation . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Both positive percentage and relative intensity were lower in transcript factor for Th 1 cytokine , T bet ( 40 . 5 % and 0 . 139 , respectively ) than those for Th 2 cytokine , GATA 3 ( 89 . 2 % and 0 . 364 , respectively ) . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| T bet / GATA 3 ratio as a measure of the Th1 / Th2 cytokine profile in mixed cell populations : predominant role of GATA 3 . ^^^ The differentiation of naive T helper ( Th ) cells towards Th 1 or Th 2 cells is regulated by the transcription factors T box expressed in T cells ( T bet ) and GATA binding protein 3 ( GATA 3 ) . ^^^ In the present study , the gene expression of T bet and GATA 3 was measured by semi quantitive reverse transcription polymerase chain reaction ( RT PCR ) in Th 1 and Th 2 cells derived from purified splenic CD4+ T cells from DO11 . 10 / Rag2 ( / ) transgenic mice and control BioBreeding ( BBc ) Wistar rat splenic T cells stimulated under Th 1 or Th 2 conditions . ^^^ In both sets of experiments , changes in the ratio of expression of T bet and GATA 3 reflected changes in the Th 1 specific cytokine interferon gamma ( IFN gamma ) and Th 2 specific cytokine interleukin ( IL ) 4 . ^^^ T bet gene expression was not maintained in fully polarized rat Th 1 cells whereas GATA 3 gene expression was maintained in long term polarized rat Th 2 cells , indicating that maintenance of Th1 / Th2 status occurred more as a result of altered GATA 3 mRNA expression than T bet . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Stat 1 is required for suppression of GATA 3 , but T bet induction by IL 27 can also be mediated through a Stat 1 independent pathway . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Further , it stabilized IL 4 production and GATA 3 expression in differentiated Th 2 cells , whereas in differentiated Th 1 cells it inhibited the expression of T bet and reduced the production of IFN gamma . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Chronic morphine treatment differentiates T helper cells to Th 2 effector cells by modulating transcription factors GATA 3 and T bet . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| To clarify the functional state of T cells in Kawasaki disease , we analysed mRNA expression levels of Th1 / Th2 cytokines ( IFN gamma and IL 4 ) along with Th1 / Th2 inducing transcription factors , T bet and GATA 3 , which play pivotal roles in the development of Th 1 and Th 2 cells , respectively . ^^^ These results suggest that the suppressed function of Th 1 and Th 2 , associated with the suppression of both T bet and GATA 3 gene expression , may be one of the immunological characteristics of Kawasaki disease . . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Different antigens trigger different Th1 / Th2 reactions in neonatal mononuclear cells ( MNCs ) relating to T bet / GATA 3 expression . ^^^ Employing Dermatophagoides pteronyssinus ( Der p ) extract and varicella zoster virus ( VZV ) as antigens , we assessed Th1 / Th2 reactions as demonstrated by IL 4 / IFNgamma production and mRNA expression , and transcriptional factors T bet / GATA 3 mRNA expression in mononuclear cells from human umbilical cord blood ( CBMC ) . ^^^ VZV enhanced Th 1 related transcription factor T bet mRNA expression , in association with later down regulation of Th 2 related GATA 3 mRNA expression in PBMC . ^^^ However , VZV did not up regulate T bet or down regulate GATA 3 expression significantly in CBMC . ^^^ In contrast , Der p induced an early GATA 3 expression and later T bet expression in CBMC . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Here , we tested the flexibility of human Th 1 or Th 2 differentiation by forced expression of transcription factors T bet and GATA 3 . ^^^ Ectopic expression of T bet and GATA 3 in freshly isolated human T ( N ) cells resulted in their differentiation to a Th 1 and Th 2 phenotype , respectively , in the absence of polarizing cytokines . ^^^ Interestingly , similar to the naive T cell subset , central memory T cells were also largely programmed toward Th 1 or Th 2 effector cells upon expression of T bet and GATA 3 , respectively . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Combining cytokine signalling with T bet and GATA 3 regulation in Th 1 and Th 2 differentiation : a model for cellular decision making . ^^^ Differentiation of uncommitted T cells into Th 1 and Th 2 subpopulations depends on both intracellular events controlling expression of transcription factors T bet and GATA 3 and interactions between cells mediated by cytokines , particularly IL 4 and IFNgamma . ^^^ It shows how antigen stimulation in conjunction with cytokines and other extracellular signals gives rise to rapid , reversible and mutually exclusive expression of T bet or GATA 3 due to feedback between the transcription factors and their signalling pathways . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Effects of ginsenoside F 3 on mRNA level of cytokines IL 4 , IFN gamma , and transcription factors T bet and GATA 3 were evaluated by RT PCR analysis . ^^^ RT PCR analysis displayed that ginsenoside F 3 enhanced the IFN gamma and T bet gene expression and decreased IL 4 and GATA 3 gene expression . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| It has been shown that cytokine signaling molecules , including transcription factors T bet and GATA 3 , interleukin 12 receptor beta 2 ( IL 12R beta 2 ) and suppressors of cytokine signaling ( SOCS ) , such as SOCS 1 , are important in differentiation of naive T cells into Th 1 helper T cells . ^^^ Expression of T bet , GATA 3 , IL 12R beta 2 and SOCS 1 was significantly increased in HAM / TSP patients in comparison with HTLV 1 uninfected individuals . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| At the onset of shock , compared to healthy volunteers , mRNA levels in septic shock patients were increased for IL 10 , IL 1beta , and high mobility group B 1 ( HMGB 1 ) and decreased for transforming growth factor beta 1 , the Th 1 , and Th 2 transcription factors , T bet and GATA 3 , respectively . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Effect of treatment of rheumatoid arthritis with infliximab on IFN gamma , IL 4 , T bet , and GATA 3 expression : link with improvement of systemic inflammation and disease activity . ^^^ OBJECTIVE : To study interferon gamma ( IFN gamma ) production and the expression of T bet and GATA 3 , the transcription factors associated with Th 1 and Th 2 , in peripheral blood mononuclear cells ( PBMC ) from patients with rheumatoid arthritis before and during infliximab treatment , so as to distinguish between a disease specific and a disease activity dependent defect . ^^^ IFN gamma concentrations in supernatants were determined by ELISA . mRNA expression of IFN gamma , IL 4 , T bet , and GATA 3 was determined by real time RT PCR in whole blood at weeks 0 and 22 . ^^^ T bet expression or T bet / GATA 3 ratio of rheumatoid blood was less than in controls . ^^^ The T bet / GATA 3 ratio was not influenced by infliximab treatment . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| The present study analyzed the expression of the following 13 TFs : AML 1 , CEBPA , E2A , ETS 1 , GATA 1 , GATA 2 , GATA 3 , IKAROS , IRF 1 , PAX 5 , PU 1 , TBET and TCF 1 in 7 malignant NK cell lines together with 5 malignant NKT cell lines , 5 T cell acute lymphoblastic leukemia ( ALL ) cell lines including 3 gamma / delta T cell receptor ( TCR ) type and 2 alpha / beta TCR type , and 3 B cell precursor ( BCP ) leukemia cell lines . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Nevertheless , after culture in either Th 1 polarizing or neutral conditions , mRNA was present for both T bet and GATA 3 as well as for IL 12Rbeta2 in WC1 . 1+ and WC1 . 2+ subpopulations , and neither produced IL 4 under any conditions . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| To investigate whether dengue 2 patients with and without dengue hemorrhagic fever had different virus load , immune mediators , or T helper ( Th ) reaction , we simultaneously measured virus load , immune mediators and the Th1 / Th2 transcription factors T bet / GATA 3 mRNA expression in a large outbreak of dengue 2 infections in Southern Taiwan . ^^^ Further studies showed that patients with dengue hemorrhagic fever had a significantly lower T bet than those with dengue fever , but GATA 3 mRNA expression in peripheral blood leukocytes was not significant difference between both groups . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| T cells primed with TGF beta secreted IFN gamma in response to both T cell receptor ligation and IL 12 / IL 18 stimulation , and expressed high levels of T bet and low levels of GATA 3 . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| These effects were associated with suppression of OVA specific IL 4 production and GATA 3 expression and with up regulation of IFN gamma production and T bet expression by splenocytes . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Expression of the T cell transcription factors , GATA 3 and T bet , in the neoplastic cells of Hodgkin lymphomas . ^^^ Out of HL cell lines , T bet was expressed in five , GATA 3 in four , and c Maf in two cell lines . ^^^ Immunohistochemistry in HL tissues revealed that in 11 of 12 ( 92 % ) of the classical HL cases HRS cells were GATA 3 and / or T bet positive . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| T helper cell fate specified by kinase mediated interaction of T bet with GATA 3 . ^^^ Cell lineage specification depends on both gene activation and gene silencing , and in the differentiation of T helper progenitors to Th 1 or Th 2 effector cells , this requires the action of two opposing transcription factors , T bet and GATA 3 . ^^^ T bet is essential for the development of Th 1 cells , and GATA 3 performs an equivalent role in Th 2 development . ^^^ We report that T bet represses Th 2 lineage commitment through tyrosine kinase mediated interaction between the two transcription factors that interferes with the binding of GATA 3 to its target DNA . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| We now report that HSP 60 in vitro differentially modulates the expression of Th1 / Th2 transcription factors in human T cells : HSP 60 down regulates T bet , NF kappaB , and NFATp and up regulates GATA 3 , leading to decreased secretion of TNF alpha and IFN gamma and enhanced secretion of IL 10 . ^^^ In BALB / c mice , HSP 60 in vivo inhibited the clinical , histological , and serological manifestations of Con A induced hepatitis associated with up regulated T cell expression of suppressor of cytokine signaling 3 and GATA 3 and down regulated T bet expression . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Of note , IFN alpha enhanced the expression of mRNA for c Maf , T bet , and Fox P 3 , irrespective of the presence of IL 12 , but not that for GATA 3 , in anti CD 3 stimulated CD4+ T cells . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| DESIGN AND METHODS : The balance of Th1 / Th2 and Tc1 / Tc2 was studied by simultaneous analysis of intracellular cytokines of peripheral blood mononuclear cells and splenocytes in short term cultures activated with PMA / ionomycin as well as mRNA expression of T bet and GATA 3 in peripheral blood mononuclear cells and splenocytes using real time polymerase chain reaction . ^^^ GATA 3 mRNA expression in ITP patients was significantly lower both in PB ( p < 0 . 01 ) and in splenocytes ( p < 0 . 01 ) than in corresponding samples from controls while there was no difference in T bet expression . ^^^ The T bet / GATA 3 ratio may provide a surrogate marker of T1 / T2 cytokine balance . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| We report that Vav 1 / CD4+ T cells display deficient T cell receptor ( TCR ) / CD28 induced IL 4 and c Maf expression and , conversely , enhanced interferon gamma ( IFN gamma ) production and T bet expression ( even when cultured under Th 2 polarizing conditions ) , but intact expression of other Th 2 cytokines and GATA 3 . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| T bet ( T box expressed in T cells ) and GATA 3 are transcription factors that play a critical role in the development of Th 1 and Th 2 cells , as do genes of the SOCS ( suppressor of cytokine signaling ) family , albeit indirectly . ^^^ T bet ( but not GATA 3 ) was expressed at significantly lower levels in cells of filaria infected individuals in response to BmA compared with those from the uninfected group , accounting , at least partially , for the diminished IFN gamma expression . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| To investigate the roles of TFs in human B cell precursors ( BCPs ) , the present study analyzed the expression of the following 16 hematopoietic TFs : AML 1 , C / EBPalpha , C / EBPbeta , C / EBPgamma , C / EBPepsilon , E2A , Ets 1 , GATA 1 , GATA 2 , GATA 3 , Ikaros , IRF 1 , Pax 5 , PU . 1 , T bet and TCF 1 in 30 human BCP leukemia cell lines . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Treatment of PLP ( 139 151 ) specific T cells in vitro with AICAR decreased their expression of T bet in response to IL 12 , a Th 1 transcription factor , whereas in response to IL 4 , it induced the expression and phosphorylation of Th 2 transcription factors GATA 3 and STAT 6 , respectively . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| RNA quantification of T bet , GATA 3 , IFN gamma , and IL 4 shows a defective Th 1 immune response in the peripheral blood from rheumatoid arthritis patients : link with disease activity . ^^^ T bet and GATA 3 transcription factors associated with Th 1 and Th 2 responses respectively , and IFNgamma and IL 4 mRNA levels were measured by real time PCR . ^^^ In comparison to RA patients with a low CRP ( CRP < 40 mg / l ) , patients with a high CRP ( CRP > or=40 mg / l ) had lower IFNgamma / beta actin , T bet / beta actin mRNA levels and T bet / GATA 3 expression ratios . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| RESULTS : The expression ratio of T bet : Gata 3 , master transcription factors for Th 1 and Th 2 cytokines , was increased in the MLN from NTP 2000 fed BBdp rats compared with that from BBc rats , mainly due to decreased Gata 3 expression . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Measurements of the transcriptional regulators of T cell polarization revealed that MMP 12 null cells had increased T bet and reduced GATA 3 expression , a condition that favors a Th 1 bias . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| However , they were comparable with the IFN gamma+ or IL 4+ cells in the expression levels of other Th1 / Th2 marker genes ( GATA 3 , Tbet , and IL12Rbeta2 ) . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| To further explore longitudinal changes in peritoneal immunity during PD related peritonitis , we examined the production of interleukin 12 ( IL 12 ) , IL 18 , and interferon gamma ( IFN gamma ) in peritoneal dialysate effluent ( PDE ) and kinetic expression of the transcription factors T box expressed in T cells ( T bet ) and guanine adenine thymine adenine ( GATA ) binding protein 3 ( GATA 3 ) in peritoneal T cells during peritonitis . ^^^ METHODS : IL 12 , IL 18 , and IFN gamma protein and IFN gamma , T bet , and GATA 3 messenger RNA ( mRNA ) were measured in PDE during various phases of peritonitis in 40 patients undergoing PD . ^^^ The kinetics of IFN gamma , T bet , and GATA 3 mRNA expression in peritoneal T cells , measured by means of real time polymerase chain reaction , differed between the 2 groups . ^^^ In the rapid response group , IFN gamma and T bet mRNA expression increased , whereas that of GATA 3 decreased over time . ^^^ Results were opposite in the delayed response group , with IFN gamma and T bet levels decreasing and GATA 3 levels increasing over time . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Effects of CpG oligodeoxynucleotide on transcription factors GATA 3 and T bet mRNA expression in asthmatic mice . ^^^ AIM : To investigate effects of CpG oligodeoxynucleotide ( CpG ODN ) on the mRNA expression of transcription factors GATA binding protein 3 ( GATA 3 ) and T box expressed in T cells ( T bet ) in asthmatic mice . ^^^ Transcription factor GATA 3 and T bet mRNA expression in splenocytes and lung tissue were detected by reverse transcription polymerase chain reaction . ^^^ GATA 3 mRNA expression was reduced in both lung tissue and splenocytes in the CpG ODN treated group , while the mRNA ratio of T bet to GATA 3 in splenocytes was increased . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Despite the difference in IFN gamma production , cells bearing either WC 1 isoform had similar transcription levels of the high affinity IL 12 receptor subunit ( IL 12Rbeta2 ) as well as of the transcription factors T bet and GATA 3 when cultured with IL 12 . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Up regulation correlated with increased expression of GATA 3 and signal transducer and activator of transcription 6 ( Stat 6 ) , Th 2 specific transcription factors and decreased expression of T bet , a transcription factor involved in Th 1 differentiation . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| In contrast to GATA 3 , NFAT , and NF kappaB , intrapair variability of T bet , the master transcription factor of Th 1 cells , was very low among monozygotic and high among dizygotic twins , indicative of a strong genetic influence on T bet ( heritability 0 . 93 , 95 % confidence interval , 0 . 84 1 . 0 ) . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Meanwhile , both positive percentage and relative intensity of gene expression were lower for a type 1 cytokine related transcription factor T bet ( 31 . 4 % and 0 . 142 , respectively ) than those for type 2 cytokine related GATA 3 ( 85 . 7 % and 0 . 378 , respectively ) . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Here , we studied T bet expression kinetics in parallel with GATA 3 during Th1 / Th2 polarization . ^^^ In addition , we show that the immunosuppressive cytokine TGF beta inhibits T bet and GATA 3 protein expression only if it is present prior to primary T cell activation and is maintained in the cultures during the early polarization of Th1 / Th2 cells . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| LLO treatment resulted in a reduction of GATA 3 and SOCS 3 expressions but induced the transcription of T bet instead . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Helper T cells from methyl CpG binding domain protein 2 deficient mice exhibit exuberant patterns of cytokine expression despite appropriate silencing of genes encoding the lineage specifying factors T bet and GATA 3 . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Using conditional introduction of dominant negative factors , we now show that T bet and GATA 3 are far more critical in establishment than maintenance of IFN gamma and IL 4 activity during Th 1 and Th 2 maturation , respectively . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Second , using artificial antigen presenting cells to specifically isolate self reactive T cells , we demonstrate that ASCT induces autoimmune cells to deviate from a proinflammatory phenotype ( mRNA interferon gamma [ IFN gamma ] and T bet high ) to a tolerant phenotype ( mRNA interleukin 10 [ IL 10 ] and GATA 3 high ) . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| The effects of allitridin on the expression of transcription factors T bet and GATA 3 in mice infected by murine cytomegalovirus . ^^^ This study was designed to investigate the effects of allitridin on the expression of transcription factors T bet and GATA 3 in mice infected by murine cytomegalovirus ( MCMV ) . ^^^ The expression levels of the transcription factors T bet and GATA 3 were measured by reverse transcription polymerase chain reaction . ^^^ MCMV infection markedly down modulated the expression of IFN gamma and T bet and significantly up modulated the expression of IL 10 and GATA 3 . ^^^ Allitridin induced significantly ( P < . 01 ) increased expression of the transcription factor T bet and the Th 1 cytokine IFN gamma and markedly ( P < . 01 ) decreased expression of the transcription factor GATA 3 and the Th 2 cytokine IL 10 . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Interestingly , the modified plasmid vaccine predominantly enhanced the type 2 immune responses manifested by an increased IgG 1 to IgG2a antibody ratio and a greater induction of GATA 3 and IL 4 mRNA than that of T bet and IFN gamma mRNA in spleen cells from vaccinated mice . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| The identification of transcription factors controlling Th 1 , Th 2 and T regulatory cell ( T ( Reg ) ) development further support the Th 2 hypothesis since GATA 3 is overexpressed and T bet underexpressed in the asthmatic airway and Foxp 3 is induced in asthma by corticosteroid treatment . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| This network encompasses 17 nodes , namely IFN gamma , IL 4 , IL 12 , IL 18 , IFN beta , IFN gammaR , IL 4R , IL 12R , IL 18R , IFN betaR , STAT 1 , STAT 6 , STAT 4 , IRAK , SOCS 1 , GATA 3 , and T bet , as well as their cross regulatory interactions . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| The levels of epsilon germline transcription and the amounts of mRNA for IL 4 , IFN gamma , GATA 3 and T bet gene in the spleen were evaluated by real time RT PCR at the end of the experiment . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Since T bet and GATA 3 are the principal transcription factors for the differentiation of type 1 and type 2 helper T lymphocytes , respectively , we studied their mRNA expression in the urinary sediment of SLE patients and compared this with their urinary and intra renal protein expression . ^^^ Urinary mRNA expression of T bet and GATA 3 were studied by the real time quantitative polymerase chain reaction . ^^^ Intra renal and urinary expressions of T bet and GATA 3 were studied by immunohistochemistry and western blotting , respectively . ^^^ For the 22 SLE patients with kidney biopsy , tubular expressions of T bet and GATA 3 significantly correlated with the histological activity index ( T bet : r = 0 . 57 , P = 0 . 006 ; GATA 3 : r = 0 . 79 , P < 0 . 001 ) . ^^^ CONCLUSIONS : Patients with active lupus nephritis have increased T bet and depressed GATA 3 expression in the urinary sediment and kidney tissue , indicating a predominant Th 1 type of T lymphocyte activation . . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Transcription factors GATA 3 and T bet , as well as its target Hlx , are absent in IL 17 producing T cells , and they do not express the negative regulator for TGFbeta signaling , Smad 7 . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Consistent with this observation , anti GITR antibody significantly enhanced the expression of the key Th 1 ( T bet ) and Th 2 ( GATA 3 ) transcription factors in vitro . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Overexpression of the transcription factor T bet in the absence of significant GATA 3 expression further assessed the type 1 polarization of aortic wall immune responses . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Cytotoxic T lymphocyte antigen 4 inhibits GATA 3 but not T bet mRNA expression during T helper cell differentiation . ^^^ Naive CD4+ T cell differentiation to T helper 1 ( Th 1 ) and Th 2 cells is dependent on T bet and GATA 3 factors , respectively . ^^^ T bet and GATA 3 , indeed , through chromatin remodelling allow transcriptional activation of Ifngamma and Th 2 cytokine ( Il 4 , Il 5 , Il 13 ) genes , respectively . ^^^ We investigated the effects of the negative costimulatory receptor cytotoxic T lymphocyte antigen 4 ( CTLA 4 ) on GATA 3 and T bet mRNA expression and Th cell differentiation in mouse naive CD4+ T cells . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| First , impaired induction of T bet and GATA 3 mRNA underlies the Th1 / Th2 deficiency in infected individuals . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Regulation of GATA 3 , c maf and T bet mRNA expression in bronchoalveolar lavage cells and bronchial biopsies after segmental allergen challenge . ^^^ BACKGROUND : GATA 3 ( GATA binding protein 3 ) and the proto oncogene c maf are Th 2 regulating transcription factors that control the expression of interleukin ( IL ) 4 and IL 5 , while T bet ( T box expressed in T cells ) is a Th 1 specific transcription factor that controls the expression of interferon ( IFN gamma ) . ^^^ METHODS : The mRNA expression of GATA 3 , c maf and T bet was determined by real time polymerase chain reaction in bronchoalveolar lavage ( BAL ) cells and bronchial biopsies . ^^^ CONCLUSIONS : Segmental allergen challenge in asthmatics leads to increased GATA 3 , c maf and T bet expression in BAL cells but not in bronchial biopsies . . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| T bet regulates Th 1 responses through essential effects on GATA 3 function rather than on IFNG gene acetylation and transcription . ^^^ T helper type 1 ( Th 1 ) development is facilitated by interrelated changes in key intracellular factors , particularly signal transducer and activator of transcription ( STAT ) 4 , T bet , and GATA 3 . ^^^ Here we show that CD4+ cells from T bet / mice are skewed toward Th 2 differentiation by high endogenous GATA 3 levels but exhibit virtually normal Th 1 differentiation provided that GATA 3 levels are regulated at an early stage by anti interleukin ( IL ) 4 blockade of IL 4 receptor ( R ) signaling . ^^^ In related studies , we show that the negative effect of GATA 3 on Th 1 differentiation in T bet / cells arises from its ability to suppress STAT 4 levels , because if this is prevented by a STAT 4 expressing retrovirus , normal Th 1 differentiation is observed . ^^^ Finally , we show that retroviral T bet expression in developing and established Th 2 cells leads to down regulation of GATA 3 levels . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Peripheral blood mononuclear cells ( PBMCs ) were isolated and cultured with CVB 4 and analyzed for cytokine and chemokine receptors by flow cytometry and for expression of transcription factors Tbet and GATA 3 by RT PCR and Western blot . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| In contrast , mRNAs encoding T cell transcription factors T bet and GATA 3 , associated with differentiation of Th 1 and Th 2 CD4+ T cells respectively , were unchanged . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Analyses of each intermediate stage for CD 34 , CD 117 , and CD 94 cell surface expression , lineage differentiation potentials , capacity for cytokine production and natural cytotoxicity , and ETS 1 , GATA 3 , and T BET expression provide evidence for a new model of human NK cell differentiation in secondary lymphoid tissues . . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Transcription factors T bet and GATA 3 regulate development of airway remodeling . ^^^ Although asthma is considered to be a Th 2 disease , the role of T bet and GATA 3 , the key transcription factors for differentiation toward Th 1 and Th 2 cells , in the pathogenesis of airway remodeling is poorly understood . ^^^ OBJECTIVES : We therefore examined the effects of GATA 3 or T bet induction of Th1 / Th2 bias on the development of airway remodeling in mice . ^^^ METHODS : The development of airway remodeling after repeated allergen challenges was analyzed using transgenic mice overexpressing either GATA 3 or T bet . ^^^ Eosinophilic airway inflammation increased in mice overexpressing GATA 3 , but decreased in mice overexpressing T bet after repeated allergen exposure . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Treatment of allergic airway inflammation and hyperresponsiveness by imiquimod modulating transcription factors T bet and GATA 3 . ^^^ Several investigators have shown that T bet and GATA 3 are master Th 1 and Th 2 regulatory transcription factors . ^^^ This study investigated whether imiquimod treatment inhibited airway inflammation by modulating transcription factors T bet and GATA 3 . ^^^ The mRNA expressions of IL 4 , IL 5 , IFN gamma , T bet and GATA 3 in lung and in CD 4 ( + ) T cells were determined by reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ The protein expressions of T bet and GATA 3 were measured by Western blot . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| These simvastatin conditioned DCs up regulated GATA 3 expression and down regulated T bet expression in cocultured CD4+ T cells in the absence of additional simvastatin added to the coculture . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| We then measured serum urea levels , performed histomorphometric analysis for tubular necrosis and regeneration , and evaluated the mRNA expression of HO 1 , t bet , Gata 3 and MCP 1 by real time PCR at 24 , 48 and 120 h after surgery . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| T bet , regulator of the Th 1 response , was up regulated in CDD fed mice , whereas Th 2 regulator GATA 3 was significantly suppressed in CDD fed mice after ConA . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| METHODS : Levels of transcripts encoding for pro and anti inflammatory markers ( tnf alpha , ifn gamma , il 10 , t bet , gata 3 ) as well as for TGF beta 1 signaling mediators ( smad 3 , smad 4 , smad 7 ) were followed by real time PCR in a prospective study enrolling 6 AGMs and 6 MACs . ^^^ In AGMs , we found increases of gata 3 but not t bet , a longer lasting up regulation of smad 4 ( days 1 21 p . i ) , a mediator enhancing TGF beta 1 signaling , and no smad 7 up regulations . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| Likewise , analysis of transcription factors shows increased T bet with respect to both allergens , but also GATA 3 with respect to Ber e 1 . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| RESULTS : We developed real time PCR based detection assays for several immune related transcription factor and cytokine genes from deer mice that permit the profiling of CD4+ helper T cells , including markers of Th 1 cells ( T bet , STAT 4 , IFNgamma , TNF , LT ) , Th 2 cells ( GATA 3 , STAT 6 , IL 4 , IL 5 ) and regulatory T cells ( Fox p 3 , IL 10 , TGFbeta 1 ) . ^^^ |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UL17 and P23771 |
Pubmed |
SVM Score :0.0 |
| NA |
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