Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.82570676
Thus , dbpA competed for the interaction between NF YA and NF YB by binding to NF YA . 0.82570676^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.59051496
These results indicate that ( 1 ) the NF Y histone fold dimer can efficiently associate DNA during nucleosome formation ; ( 2 ) it has an intrinsic affinity for H 3 H4 but does not form octamers ; and ( 3 ) the interactions between NF YA , NF YB NF YC , and H 3 H4 or nucleosomes are not mutually exclusive . 0.59051496^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.60699501
A subunit interaction domain of NF YA , which forms a heterotrimer with NF YB and NF YC , is not required for these interactions with Sp 1 or Sp 3 . 0.60699501^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :1.5005884
Whereas recombinant CBF B binds to DNA after complementation with a highly purified CBF A fraction , recombinant CBF A was unable to bind to DNA after complementation with either purified CBF B or recombinant CBF B . 1.5005884^^^ However , when recombinant CBF A , synthesized as a fusion protein with glutathione S transferase was denatured together with a highly purified fraction containing CBF A in the presence of 5 . 5 M guanidine hydrochloride and subsequently renatured , the recombinant CBF A bound to DNA after complementation with CBF B . 0.67659316^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.8345662
We show that CBF A and CBF C interact with each other to form a CBF A CBF C complex and that CBF B does not interact with CBF A or CBF C individually but that it associates with the CBF A CBF C complex . 0.8345662^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.65424732
The second class of mutants identified a segment of CBF A that is necessary for interactions between the CBF A / CBF C heterodimer and CBF B to form a CBF heterotrimer . 0.65424732^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.53155047
In this study using cross linking and immunoprecipitation methods , we first established that CBF B interacts simultaneously with both subunits of the CBF A CBF C heterodimer to form a heterotrimeric CBF molecule . 0.53155047^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.55680226
The nature of the association of CBF B with CBF A / CBF C was also revealed by studying the inhibition of CBF DNA complex formation by the mutant CBF B . 0.55680226^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.62644318
This polarity in the heterodimer interactions suggests that the interactions between HAP 2 and HAP 3 in solution are basically the same as those in the flagellar axial structure . 0.62644318^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NF YA and NF YB show stretches of arresting sequence homology to the yeast transcriptional activators HAP 2 and HAP 3 . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
We have further established that the serum dependence of TK CCAAT binding activity in normal IMR 90 fibroblasts is due to the decrement of NF Ya , but not NF Yb expression following serum deprivation , and that such serum dependence is absent in HL 60 cells . . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
The NF YA subunit contains a domain of high homology to yeast HAP 2 , which we show to be necessary and sufficient to mediate interactions with the NF YB subunit and with DNA . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
Further characterization of CBP / tk binding complex indicates that the binding complex may contain NF YA and NF YB and that the binding activity was sensitive to oxidizing reagents . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
Identification of the transcription factors NF YA and NF YB as factors A and B that bound to the promoter of the major histocompatibility complex class 2 gene 1 A beta . ^^^ The genes for the heteromeric protein NF Y ( NF YA and NF YB ) , which binds to the 1 E alpha Y box have been cloned . ^^^ We subsequently isolated the genes for NF YA and NF YB using oligonucleotides designed from the published sequences . ^^^ NF YA and NF YB were tested for binding to the 1 A beta and 1 E alpha Y boxes . ^^^ While neither NF YA or NF YB alone bound to the Y box , when the components were mixed the complex bound to the 1 A beta Y box with high affinity . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
Both NF YA and NF YB were constitutively expressed at mRNA level in IMR 90 cells . ^^^ However , expression of NF YA , and to a lesser degree , NF YB , at the protein level were clearly age dependent . ^^^ The half life of NF YA and NF YB were , respectively , 4 and > 10 fold longer than that of CBP / tk binding activity in IMR 90 cells . ^^^ Taken together , our results suggested that although CBP / tk may contain NF YA or related proteins , NF A and NF YB alone may not account for all the characteristics of CBP / tk observed in normal and transformed human cells . . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
The subunit association and DNA binding properties of the NF Y complex were examined as a function of redox state using recombinant NF YA , NF YB , and NF YC subunits . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NF Y is composed of three subunits , NF YA , NF YB and NF YC , all necessary for DNA binding . ^^^ Immunopurification of holo TFIID with anti TBP and anti TAFII 100 antibodies indicates that a fraction of NF YB associates with TFIID in the absence of NF YA . ^^^ Sedimentation velocity centrifugation experiments confirm that two pools of NF YB , and most likely NF YC , exist : one associated with NF YA and binding to the CCAAT box ; another involved in high molecular weight complexes . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
We expressed and purified the yeast homology domain of NF YC in bacteria and performed EMSA together with the corresponding conserved domains of NF YA and NF YB , obtaining a CCAAT binding mini NF Y . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
Here , we demonstrate that the Mr 30 , 000 / 40 , 000 subunits of CBP / cycA are identical with NF YA and NF YB , the two subunits of NF Y . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
The mammalian transcription factor , NF Y ( CBF ) , contains three known subunit components , NF YA ( CBF B ) , NF YB ( CBF A ) , and NF YC ( CBF C ) , which are all required to reconstitute specific CCAAT box DNA binding activity . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
The assembled NF YA : B : C complex , and the NF YB : YC , NF YB : YC ( DNA binding subunit interaction domain ) , and NF YC : YB ( DNA binding subunit interaction domain ) heterodimers were sufficient to support stable interaction with human GCN 5 in vitro , suggesting that these histone acetyltransferases interact with a unique surface in the ancient YB : YC histone fold motif . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NF Y is a transcription factor that binds to the Y box and has two constituents : NF YA ( that binds weakly to DNA ) and NF YB ( that increases the binding of NF YA to DNA ) . ^^^ The dbpA protein represses the expression of 1 A beta by a quenching mechanism , forming a complex with NF YA and the dbpB protein by sequestering the NF YB protein . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
The NF Y complex derived from class II+ mature B cells bound with high affinity to anion exchangers , and eluted as an intact trimeric complex , whereas , NF Y derived from class 2 plasma B cells , and from bare lymphocyte syndrome group 2 cell lines , RJ2 . 2 . 5 and RM 3 , dissociated into discrete NF YA and NF YB : C subunit fractions . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
We clone Xenopus NF YA , NF YB and NF YC and establish that NF Y is the predominant Y box binding protein in Xenopus oocyte nuclei . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
This region specifically bound to nuclear proteins extracted from rat hearts , and gel shift assays using competitive oligonucleotides , antibodies and recombinant proteins showed that this region binds to the NF YA and NF YB , members of the CCAAT binding nuclear protein family . ^^^ This region dependent transcription in cardiac myocytes transfected with antisense cDNAs encoding NF YA and NF YB was decreased to approximately 50 % of that seen in cells transfected with the same sense cDNAs . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
It is a trimer formed by NF YA and two putative histone like subunits , NF YB and NF YC , showing similarity to histones H2B and H2A , respectively . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
Our analysis indicates that ( 1 ) subunit interactions are normal ; ( 2 ) NF YB NF YC and NC2alpha / beta do not form heterodimers and NC 2 can not associate NF YA . ( 3 ) None of the NF Y swaps can complex with TBP on a TATA box . ( 4 ) Specific residues , R 47 and K 49 in NF YC and N 61 in NF YB , are crucial for CCAAT binding . ^^^ We conclude that specificity of the NF Y trimer is not due to NF YA only , but stems in part from the contribution of the histone fold alpha 1 , particularly that of NF YB . . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
The mRNA and protein levels of the histone fold containing NF YB and NF YC were invariant , whereas the NF YA protein , but not its mRNA , was maximal in mid S and decreased in G2 / M . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
The 10 element binds RFX ( RFX5 / RFXANK RFXB / RFXAP ) and CREB , while Y binds NF Y / CBF ( NF YA , NF YB , and NF YC ) . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
The six alternatively spliced NF YA isoforms all showed significantly less synergistic transactivation of the CBS 1b promoter with Sp 1 than wild type NF YA , as determined by cotransfections in Drosophila SL 2 cells with NF YB and NF YC . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
This element bound specific complexes containing NF YA and NF YB proteins in SH SY5Y nuclear extracts , the binding of which was augmented by 5 AzaC in association with enhanced levels of NF YB protein and the function of which was confirmed by inhibition using dominant negative NF YA . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NF Y is composed of three subunits , NF YA , NF YB , and NF YC , all required for DNA binding . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NF YA , NF YB are nuclear proteins . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NF Y is a ubiquitous CCAAT binding factor composed of NF YA , NF YB and NF YC . ^^^ Some NF YB and NF YC duplicates showed significant evidence of asymmetric evolution but not the NF YA duplicates . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
The transcriptional activator NF Y is a heterotrimeric complex composed of NF YA , NF YB , and NF YC , which specifically binds the CCAAT consensus present in about 30 % of eukaryotic promoters . ^^^ While NF YA is imported by an importin beta mediated pathway , the NF YB / NF YC heterodimer is translocated into the nucleus in an importin 13 dependent manner . ^^^ Importin 13 competes with NF YA for binding to the NF YB / NF YC dimer . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
CCAAT binding factors A ( CBF A ) and B ( CBF B ) are two subunits of the heteromeric CCAAT binding factor . ^^^ Portions of CBF A and CBF B have a high degree of amino acid sequence identity to segments of the HAP 3 and HAP 2 subunits of a yeast multimeric transcription factor . ^^^ A segment of 83 amino acids from residues 252 to 334 , which corresponds to the evolutionarily conserved portion of CBF B , is necessary and sufficient for CBF A dependent DNA binding . ^^^ Carboxyl terminal deletions of this segment ( or mutations in arginine residues in this carboxyl terminal part ) abolish DNA binding , but do not alter subunit interactions between CBF A and CBF B . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
The 90 amino acid ( aa ) segment of CBF A , which shows a high degree of sequence identity with the yeast transcription factor , HAP 3 , is split into exons 3 and 4 . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
CBF consists of three subunits , CBF A , CBF B , and CBF C , all three of which are necessary for DNA binding . ^^^ Nuclear extracts from NIH 3T3 cells were first depleted of CBF and then complemented with recombinant CBF B and a highly purified fraction containing native CBF A and CBF C . ^^^ Recombinant full length CBF B together with CBF A and CBF C activated transcription of several alpha 2 ( 1 ) collagen gene promoter constructs . ^^^ We generated a truncated form of CBF B that was still able to bind DNA in the presence of CBF A and CBF C . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
CBF A , CBF B , and CBF C together form the heterotrimeric mammalian CCAAT binding factor , CBF , which binds to DNA to form a CBF DNA complex . ^^^ Two CBF deletion mutants , one containing full length CBF A and CBF C and a CBF B lacking the NH 2 terminal residues 1 224 , and the other containing full length CBF A and CBF B and a CBF C lacking the COOH terminal residues 114 309 , also stimulated transcription from these promoters , but the level of activation was reduced to half that obtained with the full length CBF subunits . ^^^ In contrast , a CBF deletion mutant protein containing full length CBF A and deleted forms of both CBF B and CBF C showed very little transcription activation from these promoters . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
The mammalian CCAAT binding factor CBF ( NFY ) consists of three subunits , CBF A , CBF B , and CBF C . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
Genes encoding the CBFbeta subunit ( CBFB ) and one of the CBFalpha subunits ( CBFA 2 , otherwise known as AML 1 ) are the most frequent targets of chromosomal translocations in acute leukemias in humans . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
The histone fold motifs of CBF A and CBF C interact with each other to form a heterodimer that associates with CBF B to form a heterotrimeric CBF molecule , which then binds to DNA . . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
All chromosomal rearrangements involving the CBFA 2 and CBFB genes create chimeric proteins , two of which have been unequivocally demonstrated to function as transdominant negative inhibitors of CBF function . ^^^ Both the Cbfa 2 and Cbfb genes are essential for normal hematopoiesis in mice , because homozygous disruption of either gene blocks definitive hematopoiesis . ^^^ Recent data suggest that Cbfa 2 and Cbfb are required for the emergence of definitive hematopoietic stem cells in the embryo from a putative definitive hemangioblast precursor . ^^^ On the other hand , chromosomal translocations involving the CBFA 2 and CBFB genes in leukemias block hematopoiesis at later steps . ^^^ The cumulative data suggest that although the earliest requirement for Cbfa 2 and Cbfb is for emergence of definitive hematopoietic stem cells , both genes are also required at later stages in the differentiation of some hematopoietic lineages . . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
Core binding factor ( CBF ) is a heterodimeric transcription factor consisting of a DNA binding subunit ( Runx , also referred to as CBFA , AML 1 , PEBP2alpha ) and a non DNA binding subunit ( CBFB ) . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
One gene encoding a CBFalpha subunit , RUNX 1 ( also known as AML 1 , CBFA 2 , and PEBPA2A ) , and the gene encoding CBFbeta ( CBFB ) are essential for hematopoiesis and are frequently mutated in human leukemias . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
We have isolated three genes that potentially encode a HAP3 / nuclear factor YB ( NF YB ) / CCAAT binding factor A ( CBF A ) subunit of a CCAAT box binding complex in rice ( Oryza sativa ) , and named them OsHAP3A , OsHAP3B and OsHAP3C . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
In this report , we demonstrate that Hap2p , Hap3p , and Hap5p assemble via a one step pathway requiring all three subunits simultaneously , as opposed to the mammalian CCAAT binding factor which has been shown to assemble via a two step pathway with CBF A ( Hap3p homolog ) and CBF C ( Hap5p homolog ) forming a stable dimer before CBF B ( Hap2p homolog ) can interact . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
Here we report that transcription of LPD 1 requires HAP 2 , HAP 3 and HAP 4 for release from glucose repression . ^^^ In hap 2 , hap 3 and hap 4 null mutants , the specific activities of LPDH in cultures grown on galactose and raffinose showed only slight induction above the basal level on glucose medium . ^^^ Similar results were obtained upon assaying for beta galactosidase production in wild type , or hap 2 , hap 3 or hap 4 mutant strains carrying a single copy of the LPD 1 promoter fused in frame to the lacZ gene of Escherichia coli and integrated at the URA 3 locus . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
In contrast , gel mobility shift experiments have failed to reveal that HAP 2 or HAP 3 binds to domain 1 or that hap 3 mutations affect the complexes bound to it . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
In addition , we have shown that GCN 5 is also required for the activity of the HAP 2 HAP 3 HAP 4 transcriptional activation complex , which mediates the transcription of genes involved in respiratory functions . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
However , we have found that HAP 2 and HAP 3 gene products are involved in the expression of HEM 3 . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
We have used a fission yeast library to clone a homolog of S . cerevisiae HAP 2 , which along with HAP 3 and HAP 4 forms a transcriptional activation complex that binds to the CCAAT box . ^^^ The S . pombe homolog php 2 ( S . pombe HAP 2 ) was obtained by functional complementation in an S . cerevisiae hap 2 mutant and retains the ability to associate with HAP 3 and HAP 4 . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
Different 5 ' flanking regions of KGD 2 were fused to the lacZ gene of E . coli in episomal plasmids , and the resultant constructs were tested for expression of beta galactosidase in wild type yeast cells and in hap 2 and hap 3 mutants . ^^^ Results of the lacZ fusion assays indicated that transcription of KGD 2 is activated by the HAP 2 and HAP 3 proteins . ^^^ These data together with earlier reports on the regulation of the KGD 1 and LPD 1 genes for the alpha ketoglutarate and dihydrolipoyl dehydrogenases indicate that all three enzyme components of the complex are catabolite repressed and subject to positive regulation by the HAP 2 and HAP 3 proteins . . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
Budding yeast HAP 2 is required in concert with HAP 3 and HAP 4 to form a heterotrimeric CCAAT binding transcriptional activation complex at the UAS 2 element of CYC 1 . ^^^ Functional homologs of HAP 2 and HAP 3 have been conserved in HeLa cells where HAP 2 activity corresponds to a chromatographic fraction designated CP1B . ^^^ The roles of HAP 3 and HAP 4 in the heterotrimeric complex are also assessed by using lexA fusions and a fusion of the GAL 4 acidic activation domain to HAP 2 , which partially bypasses a deletion of HAP 4 but not HAP 3 . ^^^ These data indicate that subunits HAP 2 and HAP 3 are primarily responsible for site specific DNA binding by the complex , whereas the HAP 4 subunit provides the primary transcriptional activation domain . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
Simultaneous effects of mutations in the transcriptional regulatory genes , HAP 1 , HAP 2 and HAP 3 , on all respiratory cytochromes of Saccharomyces cerevisiae were determined . ^^^ In hap 2 and hap 3 strains all cytochromes were decreased proportionally to about 40 50 % of wild type values . ^^^ Apparently apocytochrome and heme biosynthesis retain coordination in hap 2 and hap 3 , but not in hap 1 , mutants . ^^^ The hap 1 mutant grew at near normal rates on glycerol , whereas hap 2 and hap 3 mutants grew very slowly . ^^^ The frequency of [ rho ] was high ( 16 18 % ) in hap 2 and hap 3 strains . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
HAP 3 and HAP 2 , which form the proximal and distal boundaries of the filament , showed few similarities to flagellin , each other , or the other axial proteins . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
The response to the carbon source was reduced dramatically when the same lacZ fusion was present in a hap 2 or hap 3 mutant . ^^^ This region contained several putative activation sites with sequences matching the core element proposed to be essential for binding of the HAP 2 and HAP 3 regulatory proteins . . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
The CYC 1 gene of Saccharomyces cerevisiae is positively regulated by the HAP 2 and HAP 3 proteins , which form a heteromeric complex that binds to a CCAAT box in the upstream activation site , UAS 2 , and which activate transcription in a nonfermentable carbon source . ^^^ In the hap 4 mutant , the binding of HAP 2 and HAP 3 ( HAP2 / 3 ) is not observed in vitro . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
Salmonella flagellar filaments comprise the following distinct parts connected in series : a curved hook composed of a single kind of subunit ( hook protein ) ; two short segments made up of hook associated proteins ( HAP 1 and HAP 3 ) ; a long helical filament composed of flagellin ; and a cap composed of HAP 2 . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
Yeast HAP 2 and HAP 3 activators both bind to the CYC 1 upstream activation site , UAS 2 , in an interdependent manner . ^^^ Transcriptional activation by the yeast CYC 1 upstream activation site UAS2UP1 requires the products of both the HAP 2 and HAP 3 regulatory genes . ^^^ We show here that both HAP 2 and HAP 3 in yeast extracts bind to UAS2UP1 and give rise to a single protein DNA complex , termed C , in nondenaturing polyacrylamide gels . ^^^ That both products are a part of complex C was shown by altering the mobility of the complex by fusing either HAP 2 or HAP 3 to beta galactosidase . ^^^ The binding of either HAP 2 or HAP 3 was abolished when synthesized in a strain mutant in the complementary HAP gene . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
Region 1 , from 230 to 200 , contains the principal activation site and responds to the trans acting regulatory loci HAP 2 and HAP 3 . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
Like mutations in HAP 2 , a mutation in HAP 3 abolishes the activity of UAS 2 and prevents growth on nonfermentable carbon sources . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
Yeast HAP 2 and HAP 3 : transcriptional activators in a heteromeric complex . ^^^ Activity of UAS 2 requires both the HAP 2 and HAP 3 activators , which bind to UAS 2 in an interdependent manner . ^^^ To distinguish whether these factors bound to UAS 2 cooperatively or formed a complex in the absence of DNA , HAP 2 and HAP 3 were tagged by gene fusion to LexA and beta galactosidase , respectively , and purified through four chromatographic steps . ^^^ The copurification of LexA HAP 2 , HAP 3 beta galactosidase , and UAS 2 binding activity shows that HAP 2 and HAP 3 associate in the absence of DNA to form a multisubunit activation complex . . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
These values are identical to those of three species of hook associated proteins ( HAPs ) , namely , HAP 1 , HAP 3 , and HAP 2 . ^^^ Furthermore , antibodies against HAP 1 , HAP 3 , and HAP 2 specifically reacted with the gene products of flaW , flaU , and flaV , respectively . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
The S . cerevisiae HAP 2 and HAP 3 gene products have been shown to recognize CCAAT containing transcription elements as a HAP2 / HAP3 multisubunit heterologous complex , or heteromer . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
The localization of hook associated proteins ( HAP 1 , HAP 2 , and HAP 3 ) in Salmonella typhimurium flagella was studied by using specific antibodies together with a second antibody conjugated with colloidal gold . ^^^ This finding supports the idea that HAP 2 acts to induce polymerization of endogenous flagellin at the filament tip , and HAP 1 and HAP 3 are junction proteins to connect hook with filament . ^^^ Analysis of the protein composition of short flagella from a mutant indicated that a single flagellum contains about 10 to 20 HAP 1 , 10 to 20 HAP 2 , and 10 to 40 HAP 3 molecules . . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
Activation of the CYC 1 upstream activation site ( UAS 2 ) and other Saccharomyces cerevisiae genes encoding respiratory functions requires the products of the regulatory loci HAP 2 and HAP 3 . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
Hook associated proteins , namely HAP 1 , HAP 2 , and HAP 3 , were separated from them , and the antibody against each HAP was prepared . ^^^ The order of disassembly of the component proteins by heat treatment of the hook structure from the filamentless mutants was ( HAP 2 , HAP 3 ) greater than HAP 1 greater than hook protein . ^^^ These observations were consistent with our layered structure model : HAP 1 , HAP 3 , and HAP 2 are assembled at the distal end of the hook in this sequence . ^^^ The locations of HAP 2 and HAP 3 in the hook filament structure were not clarified with the same procedure . . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
Levels of HAP 2 mRNA are not reduced in strains bearing a mutation at the HAP 3 locus , which is also required for expression of respiratory functions . ^^^ Models outlining possible interactions of the products of the HAP 2 and HAP 3 genes are presented . . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
Their molecular weights were 59 , 000 for HAP 1 , 53 , 000 for HAP 2 , and 31 , 000 for HAP 3 . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
In addition , the expression of AAC 2 gene requires the products of the HAP 2 and HAP 3 genes , but not that of the HAP 1 gene . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
In Salmonella typhimurium , three hook associated proteins , HAP 1 , HAP 2 and HAP 3 , are known to be essential for formation of flagellar filament . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
Previous studies have demonstrated that the HAP 2 , HAP 3 , and HAP 4 subunits of the yeast CCAAT binding factor are required for the transcriptional activation of genes containing a CCAAT box . ^^^ Furthermore , we demonstrate that purified recombinant HAP 2 , HAP 3 , and HAP 5 are able to reconstitute CCAAT binding activity in mobility shift analysis . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
The anti haptoglobin mAbs ( Hap 1 , Hap 2 , Hap 3 ) and the anti bovine hemoglobin ( Hb ) mAb ( BoHem 1 ) were characterized and tested for cross reactivity by means of enzyme linked immunosorbent assay ( ELISA ) and immunoblotting analyses . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
Mutations in HAP 2 , HAP 3 or HAP 4 block derepression of a CIT 1 lacZ gene fusion . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
MBR 1 and MBR 3 , two related yeast genes that can suppress the growth defect of hap 2 , hap 3 and hap 4 mutants . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
Three different mutations in the HAP 2 DNA binding domain are suppressed by a mutation in the HAP 3 DNA binding domain . ^^^ This HAP 3 mutation also suppresses mutations in a different region of HAP 2 which promotes subunit assembly of the complex . ^^^ These findings suggest that short regions of HAP 2 and HAP 3 comprise a hybrid DNA binding domain and that this domain can help hold the two subunits together in the CCAAT binding complex . . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
Bacterial flagellum consists of a basal body , a hook , HAP 1 ( hook associated protein 1 ) , HAP 3 , a long helical filament , and a cap ( composed of HAP 2 ) , all connected in series . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
This conclusion is based on several lines of evidence : ( 1 ) overexpression of GDH 1 can correct the growth defect of hap 2 and hap 3 mutants on ammonium sulfate as a nitrogen source , ( 2 ) Northern ( RNA ) blot analysis shows that the steady state level of GDH 1 mRNA is strongly lowered in a hap 2 mutant , ( 3 ) expression of a GDH 1 lacZ fusion is drastically reduced in hap mutants , ( 4 ) NADP GDH activity is several times lower in the hap mutants compared with that in the isogenic wild type strain , and finally , ( 5 ) site directed mutagenesis of two consensual HAP binding sites in the GDH 1 promoter strongly reduces expression of GDH 1 and makes it HAP independent . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
In yeast a single complex of three proteins , termed HAP 2 , HAP 3 , and HAP 5 , binds to this sequence , and in mammals the three components of the equivalent complex ( called variously NF Y , CBF , or CP 1 ) are also represented by single genes . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
Six of them were flagella specific : FlgK or HAP 1 ( 58 kDa ) , FliC or flagellin ( 52 kDa ) , FliD or HAP 2 ( 50 kDa ) , FlgE or hook protein ( 42 kDa ) , FlgL or HAP 3 ( 35 kDa ) and FlgD or hook cap protein ( 28 kDa ) . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
To obtain direct evidence for this , we have cloned the hap 2 , hap 3 and hap 5 genes from H . jecorina . ^^^ A . nidulans deltahap strains were functionally complemented in vitro by the overexpressed H . jecorina HAP 2 , HAP 3 and HAP 5 proteins , and they thus represent subunits of the CCAAT binding complex . ^^^ Furthermore , all three proteins ( HAP 2 , HAP 3 and HAP 5 ) were needed to bind to the CAE in the H . jecorina cbh 2 gene promoter in vitro . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
The order of haze formation with tannic acid was gliadin > HAP 3 > HAP 2 > HAP 1 > bovine serum albumin , which shows increasing haze formation with increasing proline content . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
HAp cylinders were manufactured by a combination of uniaxial powder pressing and different sintering conditions , with different percentages of microporosity : HAp 1 with 3 . 96+ / 0 . 75 % microporosity , HAp 2 with 15 . 66+ / 1 . 60 % microporosity , and HAp 3 with 29 . 72+ / 0 . 69 % microporosity . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
Three haplotypes , designated as Hap 1 ( T 207 , M 268 ) , Hap 2 ( T 207 , T 268 ) and Hap 3 ( M 207 , T 268 ) , accounted for more than 99 % of alleles . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
Ten promoter polymorphisms ( 9 previously unreported ) displayed strong linkage disequilibrium , yielding identification of three frequently observed haplotypes [ HAP 1 ( 43 % ) , HAP 2 ( 36 % ) , and HAP 3 ( 8 % ) ] . ^^^ Basal HAP 3 reporter activity was significantly elevated ( 1 . 8 fold ) but decreased to the same levels as HAP 2 and HAP 1 with increasing concentrations of sulforaphane , benzyl isothiocyanate ( BITC ) , and epigallocatechin gallate ( EGCG ) . ^^^
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA
Interacting proteins: P23511 and P25208	Pubmed	SVM Score :0.0
NA