| Interacting proteins: P22897 and O96017 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22897 and O96017 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P22897 and O96017 |
Pubmed |
SVM Score :0.68017067 |
| Mrc 1 associates with Cds 1 and is required for regulation of Cds 1 by the checkpoint kinase Rad 3 . 0.68017067^^^ |
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| Interacting proteins: P22897 and O96017 |
Pubmed |
SVM Score :0.55691959 |
| Cds 1 phosphorylation by Rad 3 Rad26 kinase is mediated by forkhead associated domain interaction with Mrc 1 . 0.55691959^^^ |
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| Interacting proteins: P22897 and O96017 |
Pubmed |
SVM Score :0.0 |
| In this study , we found that prolonged ( up to 4 days ) treatment with 6 TG ( 3microM ) resulted in a progressive phosphorylation of Chk 1 and Chk 2 in MMR ( + ) HeLa cells , correlating temporally with a drug induced G2 / M arrest . ^^^ |
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| Interacting proteins: P22897 and O96017 |
Pubmed |
SVM Score :0.0 |
| Treatment of MMR deficient cells with low TMZ concentrations failed to activate ATM and ATR and to cause phosphorylation of Chk 1 , Chk 2 , and p 53 , as well as G ( 2 ) / M arrest and apoptosis . ^^^ |
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| Interacting proteins: P22897 and O96017 |
Pubmed |
SVM Score :0.0 |
| Finally , both Chk 1 and Chk 2 interact with the MMR protein MSH 2 , and this interaction is enhanced after MNNG exposure , supporting the notion that the MMR system functions as a molecular scaffold at the sites of DNA damage that facilitates activation of these kinases . . ^^^ |
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| Interacting proteins: P22897 and O96017 |
Pubmed |
SVM Score :0.0 |
| Cell survival and mutagenesis following aflatoxin B 1 treatment was assayed in strains defective in nucleotide excision repair ( NER ) ( rad 14 ) , postreplication repair ( PRR ) ( rad 6 , rad 18 , mms 2 , and rad 5 ) , homologous recombinational repair ( HRR ) ( rad 51 and rad 54 ) , base excision repair ( BER ) ( apn 1 apn2 ) , nonhomologous end joining ( NHEJ ) ( yku 70 ) , mismatch repair ( MMR ) ( pms 1 ) , translesion synthesis ( TLS ) ( rev 3 ) , and checkpoints ( mec 1 1 , mec 1 1 rad 53 , rad 9 , and rad 17 ) . ^^^ |
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| Interacting proteins: P22897 and O96017 |
Pubmed |
SVM Score :0.0 |
| Activation of both Chk 2 and Chk 1 was independent of ATM and MMR in response to high dose MNNG , unlike the response to moderate doses of this drug . ^^^ |
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| Interacting proteins: P22897 and O96017 |
Pubmed |
SVM Score :0.0 |
| We describe a conserved protein Mrc 1 , mediator of the replication checkpoint , required for activation of ScRad 53 and SpCds 1 during replication stress . mrc 1 mutants are sensitive to hydroxyurea and have a checkpoint defect similar to rad 53 and cds 1 mutants . ^^^ Mrc 1 transduces signals of DNA replication stress to activate Rad 53 . ^^^ |
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| Interacting proteins: P22897 and O96017 |
Pubmed |
SVM Score :0.0 |
| Mrc 1 is a replication fork component whose phosphorylation in response to DNA replication stress activates Rad 53 . ^^^ Here we report that the Mediator protein Mrc 1 , which is required for normal DNA replication and for activation of Rad 53 , is present at replication forks . ^^^ Mutation of the canonical Mec 1 phosphorylation sites on Mrc 1 prevents Mrc 1 phosphorylation and blocks Rad 53 activation , but does not alter Mrc 1 ' s role in DNA replication . ^^^ Our results suggest a model whereby in response to DNA replication interference , the Mec 1 kinase is recruited to sites of replication blocks and phosphorylates a component of the DNA replication complex , Mrc 1 , thereby setting up a solid state Rad 53 activation platform to initiate the checkpoint response . . ^^^ |
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| Interacting proteins: P22897 and O96017 |
Pubmed |
SVM Score :0.0 |
| We show that , in response to 6 TG ( 3 micromol / L 10 24 hours ) , activating phosphorylation of CHK 1 at Ser 317 [ CHK 1 ( pS 317 ) ] and CHK 2 at Thr 68 [ CHK 2 ( pT 68 ) ] are induced differentially during a prolonged course ( up to 6 days ) of MMR mediated cell cycle arrests following 6 TG treatment , with CHK 1 ( pS 317 ) being induced within 1 day and CHK 2 ( pT 68 ) being induced later . ^^^ Thus , our data suggest that CHK 1 ( pS 317 ) is involved in a MMR mediated 6 TG induced G 2 arrest , whereas CHK 2 ( pT 68 ) seems to be involved in a subsequent tetraploid G 1 S checkpoint . ^^^ |
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| Interacting proteins: P22897 and O96017 |
Pubmed |
SVM Score :0.0 |
| Activation of Cds 1 is known to require the upstream kinase Rad 3 and the mediator Mrc 1 , but the biochemical mechanism of activation is not well understood . ^^^ In the first stage , Mrc 1 recruits Cds 1 to stalled replication forks by interactions between the FHA domain of Cds 1 and specific phosphorylated Rad 3 consensus sites in Mrc 1 . ^^^ |
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| Interacting proteins: P22897 and O96017 |
Pubmed |
SVM Score :0.0 |
| Fission yeast Mrc 1 ( mediator of replication checkpoint 1 ) is an adaptor checkpoint protein required for Rad 3 dependent activation of the checkpoint kinase Cds 1 in response to arrest of replication forks . ^^^ The T645A mutation strongly impairs a Cds 1 Mrc1 yeast two hybrid interaction that is dependent on a functional forkhead associated ( FHA ) domain in Cds 1 , indicating that phosphorylation of T 645 mediates Mrc 1 ' s association with Cds 1 . ^^^ Consistent with this model , the T 645 region of Mrc 1 effectively substitutes for the T 11 region of Cds 1 that is thought to be phosphorylated by Rad 3 and to mediate FHA dependent oligomerization of Cds 1 . ^^^ |
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| Interacting proteins: P22897 and O96017 |
Pubmed |
SVM Score :0.0 |
| Another pathway , involving Tel 1 and the MRX complex , is also required for full DNA damage induced Sae 2 phosphorylation , that is instead independent of the downstream checkpoint transducers Rad 53 and Chk 1 , as well as of their mediators Rad 9 and Mrc 1 . ^^^ |
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| Interacting proteins: P22897 and O96017 |
Pubmed |
SVM Score :0.0 |
| A Ddc 2 Rad53 fusion protein can bypass the requirements for RAD 9 and MRC 1 in Rad 53 activation . ^^^ |
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| Interacting proteins: P22897 and O96017 |
Pubmed |
SVM Score :0.0 |
| In the fission yeast Schizosaccharomyces pombe , Mrc 1 is a replication checkpoint adaptor protein that allows the sensor kinase Rad 3 Rad26 to activate the effector kinase Cds 1 . ^^^ |
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| Interacting proteins: P22897 and O96017 |
Pubmed |
SVM Score :0.0 |
| Activation of Rad 53 by either Mrc 1 or Rad 9 contributes to the survival of clb5Delta cells , suggesting that both DNA replication and damage pathways are responsive to the decreased origin usage . ^^^ |
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| Interacting proteins: P22897 and O96017 |
Pubmed |
SVM Score :0.0 |
| In telomerase negative cells deleted for RAD 9 , Mrc 1 , another checkpoint adaptor previously implicated in the DNA replication checkpoint , mediated Rad 53 activation . ^^^ However , unexpectedly , given the formation of an active Rad 53 Mrc1 complex in tlc1Delta rad9Delta cells , Mrc 1 did not mediate the cell cycle arrest elicited by telomerase loss . ^^^ |
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| Interacting proteins: P22897 and O96017 |
Pubmed |
SVM Score :0.0 |
| The budding yeast proteins Mrc 1 and Tof 1 associate with the putative MCM Cdc 45 helicase and limit progression of the replisome when nucleotides are depleted , and the checkpoint kinases Mec 1 and Rad 53 stabilize such stalled forks and prevent disassembly of the replisome . ^^^ |
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| Interacting proteins: P22897 and O96017 |
Pubmed |
SVM Score :0.0 |
| Mrc 1 and Tof 1 promote replication fork progression and recovery independently of Rad 53 . ^^^ |
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