| Interacting proteins: Q96B97 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q96B97 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q96B97 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q96B97 and P22681 |
Pubmed |
SVM Score :0.69351449 |
| CIN 85 was basally associated with c Cbl . 0.69351449^^^ For interaction of CIN 85 with c Cbl , the second SH 3 domain of CIN 85 was shown to serve as a central player . 0.51099076^^^ |
|
| Interacting proteins: Q96B97 and P22681 |
Pubmed |
SVM Score :0.8391573 |
| Monoubiquitination of CIN 85 required direct interactions between CIN 85 and Cbl , the intact RING finger domain of Cbl and a ubiquitin acceptor site present in the carboxyl terminus of CIN 85 . 0.8391573^^^ |
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| Interacting proteins: Q96B97 and P22681 |
Pubmed |
SVM Score :0.0 |
| Together , the data suggest that the interaction of Cbl carboxy terminus with CIN 85 has a minor and a redundant role in EGFR internalization . ^^^ |
|
| Interacting proteins: Q96B97 and P22681 |
Pubmed |
SVM Score :0.0 |
| Because CD2AP is closely related to Cbl interacting protein of 85 kDa ( CIN 85 ) , which regulates growth factor receptor down regulation via complex formation with Cbl and endophilin , we investigated whether the CD2AP cortactin complex performs a similar function . ^^^ |
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| Interacting proteins: Q96B97 and P22681 |
Pubmed |
SVM Score :0.0 |
| The emerging importance of the ubiquitin ligase Cbl and the adaptor molecule CIN 85 ( Cbl interacting protein of 85 kDa ) in the regulation of these pathways is discussed in detail . . ^^^ |
|
| Interacting proteins: Q96B97 and P22681 |
Pubmed |
SVM Score :0.0 |
| Upon ligand induced activation of EGF receptors , Cbl ( ubiquitin ligase ) binds to the activated receptor and leads to translocation of the CIN 85 ( Cbl interacting protein of 85 kDa ) / endophilin complex in the vicinity of the activated EGF receptors . ^^^ |
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| Interacting proteins: Q96B97 and P22681 |
Pubmed |
SVM Score :0.0 |
| CIN 85 SH3A and CIN85 / CD2BP3 SH3B bind to proline rich segments within CIN85 / CD2BP3 themselves as evidenced by mAb accessibility analysis and protein interaction studies including c Cbl binding . ^^^ |
|
| Interacting proteins: Q96B97 and P22681 |
Pubmed |
SVM Score :0.0 |
| Interactions with SH 2 domain adapters [ growth factor receptor binding protein 2 ( Grb 2 ) , Cbl , Slp 76 ] and SH 3 domain adapters ( HS 1 , cortactin , CD2BP3 ) were attenuated by inhibition of Kit kinase activity . ^^^ |
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| Interacting proteins: Q96B97 and P22681 |
Pubmed |
SVM Score :0.0 |
| Cbl CIN 85 endophilin complex mediates ligand induced downregulation of EGF receptors . ^^^ Cbl rapidly recruits CIN 85 ( Cbl interacting protein of 85K ; ref . 6 ) and endophilins ( regulatory components of clathrin coated vesicles ) to form a complex with activated EGF receptors , thus controlling receptor internalization . ^^^ CIN 85 was constitutively associated with endophilins , whereas CIN 85 binding to the distal carboxy terminus of Cbl was increased on EGF stimulation . ^^^ |
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| Interacting proteins: Q96B97 and P22681 |
Pubmed |
SVM Score :0.0 |
| CIN 85 participates in Cbl b mediated down regulation of receptor tyrosine kinases . ^^^ CIN 85 was shown to bind to the minimal binding domain identified in the carboxyl terminus of Cbl b . ^^^ Ligand induced phosphorylation of Cbl b further increased their interactions and led to a rapid and sustained recruitment of CIN 85 in the complex with EGF or PDGF receptors . ^^^ Inhibition of binding between CIN 85 and Cbl b was sufficient to impair Cbl b mediated internalization of EGF receptors , while being dispensable for Cbl b directed polyubiquitination of EGF receptors . ^^^ Moreover , CIN 85 and Cbl / Cbl b were constitutively associated with activated PDGF , EGF , or c Kit receptors in several tumor cell lines . ^^^ |
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| Interacting proteins: Q96B97 and P22681 |
Pubmed |
SVM Score :0.0 |
| Confirmed by mutagenesis and in vitro binding experiments , the novel consensus allowed for the accurate mapping of CIN 85 SH3 binding sites within known CIN 85 interactors , c Cbl , BLNK , Cbl b , AIP1 / Alix , SB 1 , and CD 2 proteins , as well as the prediction of CIN 85 novel interacting partners in protein databases . ^^^ |
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| Interacting proteins: Q96B97 and P22681 |
Pubmed |
SVM Score :0.0 |
| Identification of a novel proline arginine motif involved in CIN 85 dependent clustering of Cbl and down regulation of epidermal growth factor receptors . ^^^ CIN 85 is a multidomain adaptor protein implicated in Cbl mediated down regulation of receptor tyrosine kinases . ^^^ CIN 85 binding to Cbl is increased after growth factor stimulation and is critical for targeting receptor tyrosine kinases to clathrin mediated endocytosis . ^^^ This motif was indispensable for CIN 85 binding to Cbl / Cbl b , to other CIN 85 SH3 domains ' effectors , and for mediating an intramolecular interaction between the SH 3 A domain and the proline rich region of CIN 85 . ^^^ Individual SH 3 domains of CIN 85 bound to PXXXPR peptides of Cbl / Cbl b with micromolar affinities , whereas an extended structure of two or three SH 3 domains bound with higher stoichiometry and increased affinity to the same peptides . ^^^ |
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| Interacting proteins: Q96B97 and P22681 |
Pubmed |
SVM Score :0.0 |
| Dab 2 and clathrin dissociated from CIN 85 following growth factor treatment , enabling other molecules , such as Cbl , to bind to CIN 85 . ^^^ |
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| Interacting proteins: Q96B97 and P22681 |
Pubmed |
SVM Score :0.0 |
| CIN 85 is a multidomain adaptor protein involved in Cbl mediated down regulation of epidermal growth factor ( EGF ) receptors . ^^^ CIN 85 src homology 3 domains specifically bind to a proline arginine ( PxxxPR ) motif in Cbl , and this association seems to be important for EGF receptor endocytosis . ^^^ |
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| Interacting proteins: Q96B97 and P22681 |
Pubmed |
SVM Score :0.0 |
| No strict requirement exists for either ubiquitin modified EGFR or the Cbl binding ubiquitination substrate CIN 85 as docking site for the UIM of Eps 15 . ^^^ |
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| Interacting proteins: Q96B97 and P22681 |
Pubmed |
SVM Score :0.0 |
| However , CIN 85 did not bind directly to the cytoplasmic domain of TNFR 1 ( TNFR 1 CYT ) but to Src family kinases , Cbl and the p85alpha subunit of phosphatidylinositol 3 kinase ( PI 3 K p85alpha ) . ^^^ |
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| Interacting proteins: Q96B97 and P22681 |
Pubmed |
SVM Score :0.0 |
| The interaction between EGFR and the ubiquitin ligase Cbl / adaptor protein CIN 85 , as well as ESCRT complex recruitment play important roles in the process of downregulating EGFR . ^^^ |
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| Interacting proteins: Q96B97 and P22681 |
Pubmed |
SVM Score :0.0 |
| Herpes simplex virus 1 infected cell protein 0 forms a complex with CIN 85 and Cbl and mediates the degradation of EGF receptor from cell surfaces . ^^^ We report that an uncharted domain of ICP 0 located between residues 245 and 510 contains multiple SH 3 domain binding motifs similar to those required for binding to CIN 85 , the M ( r ) 85 , 000 protein that interacts with Cbl . ^^^ CIN 85 and Cbl are involved in endocytosis and negative regulation of numerous receptor tyrosine kinases . ^^^ We report that ICP 0 binds CIN 85 in a reciprocal manner and that the complexes pulled down by ICP 0 also contain Cbl . ^^^ |
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| Interacting proteins: Q96B97 and P22681 |
Pubmed |
SVM Score :0.0 |
| Here , we show that Sprouty 2 associates with CIN 85 and acts at the interface between Cbl and CIN 85 to inhibit EGFR downregulation . ^^^ Intact association between Sprouty 2 , Cbl and CIN 85 is required for inhibition of EGFR endocytosis as well as EGF induced differentiation of PC 12 cells . ^^^ Sprouty 2 therefore acts as an inducible inhibitor of EGFR downregulation by targeting both the Cbl and CIN 85 pathways . . ^^^ |
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| Interacting proteins: Q96B97 and P22681 |
Pubmed |
SVM Score :0.0 |
| In an effort to identify new molecular mechanisms involved in attenuating Fc epsilonRI expression and signaling , we focused our attention on CIN 85 , a scaffold molecule that regulates , in concert with the ubiquitin ligase Cbl , the clathrin mediated endocytosis of several receptor tyrosine kinases . ^^^ In the present study , we show that endogenous CIN 85 is recruited in Cbl containing complexes after engagement of the Fc epsilonRI on a mast cell line and drives ligand induced receptor internalization . ^^^ |
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| Interacting proteins: Q96B97 and P22681 |
Pubmed |
SVM Score :0.0 |
| Their function is modulated through interactions with regulatory proteins including CIN 85 and PIX , which recognize a proline arginine motif in Cbl and thus promote or inhibit receptor endocytosis . ^^^ We report the structures of SH 3 domains of CIN 85 and beta PIX in complex with a proline arginine peptide from Cbl b . ^^^ Moreover , ternary complexes of CIN 85 and Cbl are formed in vivo and are important for the ability of Cbl to promote epidermal growth factor receptor ( EGFR ) downregulation . ^^^ |
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| Interacting proteins: Q96B97 and P22681 |
Pubmed |
SVM Score :0.0 |
| The EGFR mutants were constitutively associated with the E 3 ubiquitin ligase Cbl but did not associate with the adaptor protein CIN 85 on the addition of ligand . ^^^ |
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