| Interacting proteins: Q14825 and P22681 |
Pubmed |
SVM Score :0.0 |
| While the chromosomal map is still not a complete circle , many gene loci have been mapped , some with three factor crosses , and have been characterized by enzyme assays ( leu , trp , hem , cob , cbl ) as well as complementation with B . subtilis genes ( trp , dnaK , pur , met , ssp ) E . coli genes ( ATPase ) , hybridization ( spoVG , abrB , sigK , ssp ) , and by growth on intermediates ( trp , leu , thy , gua , ade , pur , pyr , his , arg ) . ^^^ |
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| Interacting proteins: Q14825 and P22681 |
Pubmed |
SVM Score :0.0 |
| TC 2 Cbl did not restore cell division in the absence of Met by virus transformed lymphoblasts from a child with defective Met synthesis from Hcy . ^^^ The TC 2 did not act by enhanced induction of the Cbl dependent methionine synthase activity of cell extracts but the ability of intact cells to produce Met from Hcy by the Cbl dependent process appeared to have a role in the TC 2 effect . . ^^^ |
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| Interacting proteins: Q14825 and P22681 |
Pubmed |
SVM Score :0.0 |
| Efficient cellular transformation by the Met oncoprotein requires a functional Grb 2 binding site and correlates with phosphorylation of the Grb 2 associated proteins , Cbl and Gab 1 . ^^^ Moreover , we show that tyrosine phosphorylation of the Cbl protooncogene product as well as Gab 1 required Tyr 489 and correlated with the ability of Tpr Met to associate with Grb 2 and to transform cells , providing evidence that pathways downstream of Gab 1 and / or Cbl may play a role in Tpr Met mediated cell transformation . . ^^^ |
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| Interacting proteins: Q14825 and P22681 |
Pubmed |
SVM Score :0.0 |
| The proto oncogene c Cbl is a positive regulator of Met induced MAP kinase activation : a role for the adaptor protein Crk . ^^^ We report here that c Cbl , which is a Gab 1 like docking protein , also becomes tyrosine phosphorylated in response to Met activation and serves as a docking molecule for various SH 2 containing molecules , including Crk . ^^^ We further show that Cbl is similarly capable of enhancing Met induced JNK activation , and several lines of experimentation suggests that it does so by interacting with Crk . ^^^ We also show that both Cbl and Gab 1 enhance Met induced activation of another MAP kinase cascade , the ERK pathway , in a Crk independent manner . ^^^ Taken together , our studies demonstrate a previously unidentified functional role for Cbl in Met signaling and suggest that Met utilizes at least two docking proteins , Gab 1 and Cbl , to activate downstream signaling pathways . ^^^ |
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| Interacting proteins: Q14825 and P22681 |
Pubmed |
SVM Score :0.0 |
| In order to better understand Met pathway regulation , a cDNA encoding cystathionine beta lyase ( CbL ; EC 4 . 4 . 1 . 8 ) has been cloned from Solanum tuberosum . ^^^ Metabolite analysis revealed a reduction in Met levels in these CbL antisense plants , as well as remarkable increases in the pathway intermediates cystathionine , homoserine and cysteine . ^^^ This phenotype could be alleviated upon Met supplementation , suggesting that low Met levels , rather than pathway intermediate accumulation , is responsible for the phenotypic effects of CbL transgene expression . ^^^ These data unequivocally demonstrate the central role of CbL in Met biosynthesis , and , subsequently , in plant growth and development . . ^^^ |
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| Interacting proteins: Q14825 and P22681 |
Pubmed |
SVM Score :0.0 |
| In Met transformed cells , Crk predominantly associates with the Cbl and Gab1docking proteins in a tyrosine phosphorylation dependent manner . ^^^ |
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| Interacting proteins: Q14825 and P22681 |
Pubmed |
SVM Score :0.0 |
| This failure to meet all needs for Met via endogenous synthesis , which is characteristic of oncogenic transformation , occurred even in the presence of adequate homocysteine , methylfolate ( 5 CH 3 H4PteGlu ) and cobalamin ( Cbl ) dependent methionine synthetase activity . ^^^ Folinic acid ( 5 CHO H4PteGlu ) , which provides available folate independently of Cbl , improved growth only slightly in the absence of Met . ^^^ Free Cbl at 222 nM , an amount great enough to alter other intracellular events , failed to increase growth in the absence of Met , but 0 . 22 nM Cbl bound to transcobalamin 2 did , however , enhance growth . . ^^^ |
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| Interacting proteins: Q14825 and P22681 |
Pubmed |
SVM Score :0.0 |
| Ubiquitination of Met through the E 3 ligase Cbl is required for receptor downregulation , and a mutant receptor defective in Cbl binding is able to transform cells . ^^^ |
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| Interacting proteins: Q14825 and P22681 |
Pubmed |
SVM Score :0.0 |
| Remarkably , the ubiquitin ligase Cbl is rapidly recruited to InlB activated Met . ^^^ Recent studies have shown that ligand dependent endocytosis of Met and other receptor tyrosine kinases is triggered by monoubiquitination of the receptor , a process that is mediated by Cbl . ^^^ Here , we show that purified InlB induces the Cbl dependent monoubiquitination and endocytosis of Met . ^^^ First , we show that L . monocytogenes colocalizes with Met , EEA 1 , Cbl , clathrin and dynamin during entry . ^^^ |
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| Interacting proteins: Q14825 and P22681 |
Pubmed |
SVM Score :0.0 |
| Down regulation of the Met receptor involves ligand induced internalization , ubiquitination by Cbl ubiquitin ligases , and lysosomal degradation . ^^^ These results demonstrate that Cbl dependent ubiquitination is dispensable for Met internalization but is critical to target the Met receptor to components of the lysosomal sorting machinery and to suppress its inherent transforming activity . . ^^^ |
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| Interacting proteins: Q14825 and P22681 |
Pubmed |
SVM Score :0.0 |
| Here we report the identification of somatic intronic mutations of Met kinase that lead to an alternatively spliced transcript in lung cancer , which encodes a deletion of the juxtamembrane domain resulting in the loss of Cbl E 3 ligase binding . ^^^ |
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| Interacting proteins: Q14825 and P22681 |
Pubmed |
SVM Score :0.0 |
| The pyridoxal 5 ' phosphate dependent enzyme cystathionine beta lyase ( CBL ) catalyzes the penultimate step in the de novo biosynthesis of Met in microbes and plants . ^^^ |
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| Interacting proteins: Q14825 and P22681 |
Pubmed |
SVM Score :0.0 |
| Mutation of the c Cbl TKB domain binding site on the Met receptor tyrosine kinase converts it into a transforming protein . ^^^ In testing this , we show that c Cbl promotes ubiquitination of the Met RTK . ^^^ This requires the c Cbl tyrosine kinase binding ( TKB ) domain and a juxtamembrane tyrosine residue on Met . ^^^ This tyrosine provides a direct binding site for the c Cbl TKB domain , and is absent in the rearranged oncogenic Tpr Met variant . ^^^ |
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| Interacting proteins: Q14825 and P22681 |
Pubmed |
SVM Score :0.0 |
| Cbl is involved in Met signaling in B cells and mediates hepatocyte growth factor induced receptor ubiquitination . ^^^ In addition , we demonstrate that c Cbl mediates HGF induced ubiquitination of Met . ^^^ In contrast to wild type c Cbl , the transforming mutants 5 Cbl and 70Z / 3 Cbl , which lack the ubiquitin ligase RING finger domain , suppress Met ubiquitination . ^^^ Our findings identify c Cbl as a negative regulator of HGF / Met signaling in B cells , mediating ubiquitination and , consequently , proteosomal degradation of Met , and suggest a role for Cbl in Met mediated tumorigenesis . . ^^^ |
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| Interacting proteins: Q14825 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q14825 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q14825 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q14825 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
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