| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.54805972 |
| Initially , it causes an increase in the association between scFv Syk and the cytosolic protein Cbl and subsequently promotes tyrosine phosphorylation of Cbl . 0.54805972^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.72196815 |
| Tyr 316 of Syk was required for the interaction with Cbl as well as for the maximal tyrosine phosphorylation of Cbl . 0.72196815^^^ In the present study , we used genetic and biochemical approaches to demonstrate that Cbl directly interacts with Syk and Fyn via its N terminal and C terminal regions , respectively . 0.66798193^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.56823763 |
| In heterogeneous COS 1 cells , Cbl b was phosphorylated on tyrosine residues by both Syk ( Syk / Zap 70 ) and Src ( Fyn / Lck ) family kinases , with Syk kinase inducing the most prominent effect . 0.56823763^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.71469903 |
| Although the phosphorylated Btk SH 3 domain still binds c Cbl , it no longer binds WASP and instead acquires a high affinity for kinase active Syk . 0.71469903^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.59981664 |
| We also identify c Cbl as the E 3 ligase responsible for both Syk and receptor ubiquitination . 0.59981664^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.64423527 |
| We propose that c Cbl plays a regulatory role in glycoprotein 6 ( GPVI ) / Fc receptor gamma ( FcRgamma ) chain dependent platelet activation through its interaction with Syk . . 0.64423527^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| Studies with lyn or syk negative chicken B cells demonstrated that p53 / 56lyn , but not p72syk , was crucial for tyrosine phosphorylation of p120c cbl upon stimulation of the BCR . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| Co stimulation of T cells with CD 2 augments TCR CD 3 mediated activation of protein tyrosine kinase p72syk , resulting in increased tyrosine phosphorylation of adapter proteins , Shc and Cbl . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| B cell activation also induced a prominent association of Cbl with Syk tyrosine kinase . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| Similarly , Syk and SykB , but not Zap 70 , caused prominent tyrosine phosphorylation of p 120 ( c ) cbl in COS 1 cells . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| Recently , we and others have reported the activation dependent association of Cbl with the Syk and ZAP 70 tyrosine kinases through presently undefined mechanisms . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| Characterization of Cbl tyrosine phosphorylation and a Cbl Syk complex in RBL 2H3 cells . ^^^ We show that at least part of Fc epsilon R 1 induced Cbl tyrosine phosphorylation is mediated by the Syk tyrosine kinase , and that the Syk dependent tyrosine phosphorylation of Cbl occurs mainly distal to the Cbl proline rich region within the COOH terminal 250 amino acids . ^^^ Furthermore , we show by coprecipitation that Cbl is present in a complex with Syk before receptor engagement , that the proline rich region of Cbl and a region of Syk comprised of the two SH 2 domains and intradomain linker are required for formation of the complex , and that little or no tyrosine phosphorylated Cbl is detected in complex with Syk . ^^^ Overexpression of truncation mutants of Cbl capable of binding Syk has the effect of blocking tyrosine phosphorylation of endogenous Cbl . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| Stimulation of surface CD 28 molecules on mast cells with anti CD 28 mAbs induced tyrosine phosphorylation of cellular proteins , including several protein tyrosine kinases and their substrates , such as Itk / Emt ( Emt ) , Btk , Syk , c Cbl , Shc , and Vav . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| Thus , cotransfection of COS cells with Cbl plus Lck and / or Syk family protein tyrosine kinases caused a marked increase in the phosphotyrosine content of Cbl without a concomitant enhancement of its association with 14 3 3 . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| The product of the proto oncogene c cbl : a negative regulator of the Syk tyrosine kinase . ^^^ Regulation of Syk function occurred through interaction with the Cbl protein , itself a PTK substrate in this system . ^^^ Overexpression of Cbl led to inhibition of Syk and suppression of serotonin release from mast cells , demonstrating its ability to inhibit a nonreceptor tyrosine kinase . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| Convulxin induces signal transduction in part like collagen , involving the time dependent tyrosine phosphorylation of Fc receptor gamma chain , phospholipase Cgamma 2 , p 72 ( SYK ) , c Cbl , and p 36 38 . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| CD 38 ligation also induced tyrosine phosphorylation and activation of intracellular substrates , including syk , phospholipase C gamma , c cbl , and phosphatidylinositol 3 kinase ( PI 3 K ) . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| CD 38 ligation also triggers tyrosine phosphorylation of syk , c cbl , and phospholipase C gamma and activates phosphatidylinositol 3 kinase ( PI 3 K ) . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| The responses affected include the stimulation of tyrosine phosphorylation , activation of the tyrosine kinase syk , tyrosine phosphorylation of the proto oncogene Cbl , mobilization of calcium , and stimulation of the activity of phospholipase D and of the production of superoxide anions . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| Fyn , Yes , and Syk phosphorylation sites in c Cbl map to the same tyrosine residues that become phosphorylated in activated T cells . ^^^ Coexpression experiments in COS cells demonstrate that among T cell expressed Src and Syk related protein tyrosine kinases , Fyn , Yes , and Syk appear to play a major role in phosphorylation of c Cbl , whereas Lck and Zap phosphorylate c Cbl ineffectively . ^^^ Fyn , Yes , and Syk phosphorylate the same sites of c Cbl that become phosphorylated in stimulated T cells . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| We found that on cross linking of Fc epsilonRI : 1 ) HCMC released histamine ; 2 ) rapid tyrosine phosphorylation of multiple cellular substrates , including Syk , HS 1 , c Cbl , ERK 1 , and ERK 2 , was observed ; 3 ) intracellular Ca2+ and inositol phosphate production were increased within the first minute after Fc epsilonRI cross linking ; and 4 ) genistein , a tyrosine kinase inhibitor , inhibited both protein tyrosine phosphorylation and histamine release in a dose dependent manner . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| These data demonstrate the dependence of DAP 12 coupled receptors on Syk and suggest that the outcome of Ly49D / DAP12 engagement will be regulated by Cbl and culminate in the activation of transcription factors . . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| Additionally , Syk and Vav 1 cooperatively induced activation of Jun N terminal kinase ( JNK ) , extracellular signal regulated kinase 2 ( ERK 2 ) and the kinase Akt , and phosphorylation of the oncoprotein Cbl in fibrinogen adherent cells . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| Aggregation of FcgammaRIIa , the low affinity receptor for monomeric IgG , activates nonreceptor protein tyrosine kinases such as Lyn , Hck , and Syk , potentially driving the phosphorylation of the downstream adaptor proteins , including Cbl and / or Nck . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| Cbl mediated negative regulation of the Syk tyrosine kinase . ^^^ A critical role for Cbl phosphotyrosine binding domain binding to Syk phosphotyrosine 323 . ^^^ The proto oncogene product Cbl has emerged as a potential negative regulator of the Syk tyrosine kinase ; however , the nature of physical interactions between Cbl and Syk that are critical for this negative regulation remains unclear . ^^^ Here we show that the phosphotyrosine binding ( PTB ) domain within the N terminal transforming region of Cbl ( Cbl N ) binds to phosphorylated Tyr 323 in the linker region between the Src homology 2 and kinase domains of Syk , confirming recent results by another laboratory using the yeast two hybrid approach ( Deckert , M . , Elly , C . , Altman , A . , and Liu , Y . ^^^ A PTB domain inactivating point mutation ( G306E ) , corresponding to a loss of function mutation in the Caenorhabditis elegans Cbl homologue SLI 1 , severely compromised Cbl N / Syk binding in vitro and Cbl / Syk association in transfected COS 7 cells . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation and activation independent association of the tyrosine kinase Syk and the tyrosine kinase substrates Cbl and Vav with tubulin in B cells . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| One of the prominent substrates for these kinases is the adaptor protein Cbl [ 3 ] and recent studies suggest that Cbl negatively regulates upstream kinases such as Syk and Zap 70 [ 4 ] [ 5 ] . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| Cross linking of CD 43 induced tyrosine phosphorylation of several intracellular molecules including the protein tyrosine kinase Syk , the proto oncogene product Cbl , and phospholipase C ( PLC ) gamma 2 in MO7e cells . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| Role of Syk in Fc gamma receptor coupled tyrosine phosphorylation of Cbl in a manner susceptible to inhibition by protein kinase C . ^^^ Syk proved to be the most important tyrosine kinase bound to Cbl that served as the major substrate ; essentially no tyrosine phosphorylation occurred in the anti Cbl immunoprecipitates prepared from the cell lysate that had been depleted of Syk by prior immunoprecipitation with anti Syk antibodies . ^^^ Thus , protein kinase C induced serine phosphorylation of Cbl suppressed its tyrosine phosphorylation by Syk as a result of tyrosine kinase inhibition by unknown mechanisms , leading to inhibition of Cbl mediated signaling such as phosphatidylinositol 3 kinase activation . . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| Whereas CD 19 does not appear to be involved in this SYK dependent pathway , the SYK substrate CBL is likely involved as the dominant negative SYK markedly attenuates CBL tyrosine phosphorylation and completely blocks the BCR dependent association of CBL with p 85 PI3K . . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| Inhibition of signaling through the B cell antigen receptor by the protooncogene product , c Cbl , requires Syk tyrosine 317 and the c Cbl phosphotyrosine binding domain . ^^^ The ability of overexpressed c Cbl to inhibit signaling requires both Syk tyrosine 317 and a functional c Cbl PTB domain . ^^^ Coimmunoprecipitation experiments indicate that Syk phosphotyrosine 317 and the c Cbl PTB domain enhance , but are not required for , all interactions between these two proteins . ^^^ In unstimulated cells , c Cbl and Syk can be isolated in a complex that also contains tubulin . ^^^ These studies indicate that c Cbl may contribute to the regulation of BCR signaling by modulating the ability of Syk to associate with the BCR and couple the receptor to intracellular signaling pathways . . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| Cbl 70Z did not affect tyrosine phosphorylation or in vitro kinase activity of Syk , indicating that Syk may not be the direct target of Cbl 70Z in the enhancement of phagocytosis . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| This tyrosine in the linker region of Syk is a possible site for binding by the negative regulator Cbl . ^^^ The Fc epsilon RI induced phosphorylation of Cbl was downstream of Syk kinase activity and was unchanged by expression of the Y317F mutation . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| Platelet alpha IIb beta 3 integrin engagement induces the tyrosine phosphorylation of Cbl and its association with phosphoinositide 3 kinase and Syk . ^^^ Both thrombin and antibodies to alpha IIb beta 3 induced association of Cbl with the tyrosine kinase , Syk . ^^^ Experiments performed with inhibitors of tyrosine kinases indicated that both Src family kinases and Syk contribute to phosphorylation of Cbl and its consequent association with PI 3 kinase . ^^^ The results show that , following integrin alpha IIb beta 3 engagement , Cbl is tyrosine phosphorylated , recruits PI 3 kinase to this integrin signalling pathway and possibly enhances PI 3 kinase activity , downstream of Src family tyrosine kinases and Syk activation . . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| The inhibitory effect of c Cbl depended upon its N terminal phosphotyrosine binding domain , the domain that has been shown to be required for inhibition of the Syk / ZAP 70 family kinases . ^^^ Thus , CD 2 mediated NF AT transcriptional activity appears to depend upon ZAP 70 / Syk kinases and to be negatively regulated by c Cbl . . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| The rat anti CD 9 mAb , KMC8 . 8 , but not the F ( ab ' ) ( 2 ) , induced tyrosine phosphorylation of proteins including syk and cbl and induced cell aggregation in the mouse macrophage cell line , J 774 , suggesting that co cross linking of CD 9 and Fc gamma R was required for the signal . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| Basal activities of Lyn , Syk , and PI3 ' kinase were elevated in the 5 Cbl line . ^^^ CONCLUSIONS : Neither increased Bcl 2 promoter activity nor decreased ubiquitination of Bcl 2 could account for increased Bcl 2 levels . 5 Cbl expressing 32Dcl3 cells were resistant to differentiation . 5 Cbl suppresses apoptosis and differentiation , possibly through enhancement of Lyn , Syk , and PI3 ' kinase activities and Bcl 2 . . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| The RING finger domain of Cbl is essential for negative regulation of the Syk tyrosine kinase . ^^^ The proto oncogene product Cbl has emerged as a negative regulator of a number of protein tyrosine kinases , including the ZAP 70 / Syk tyrosine kinases that are critical for signaling in hematopoietic cells . ^^^ The evolutionarily conserved N terminal tyrosine kinase binding domain is required for Cbl to associate with ZAP 70 / Syk and for their subsequent negative regulation . ^^^ Analysis of a series of C terminally truncated Cbl mutants revealed that the N terminal half of the protein , including the TKB and RING finger domains , was sufficient to mediate negative regulation of Syk . ^^^ Further truncations , which delete the RING finger domain , abrogated the negative regulatory effects of Cbl on Syk . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| After a 2 min incubation with 10 6 M calyculin , a transient tyrosine phosphorylation of a subset of proteins , among which Cbl and Syk , was observed . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| G CSF induced the tyrosine phosphorylation of Cbl and increased activity of PI 3 kinase in wild type and syk deficient , but non in lyn deficient , cells . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| FcgammaRIIA ligation in RS 4 ; 11 and 380 cells induced tyrosine phosphorylation of CD 32 , CD 19 , CBL , SYK , P 13 K p 85 and SHIP , as well as RasGAP association with tyrosine phosphorylated p 62 ( dok ) . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| These data represent the first direct evidence that SHP 1 is involved in the regulation of Fcgamma receptor mediated phagocytosis and suggest that activating signals mediated by SRC family kinases SYK , CBL , phosphatidyl inositol 3 ( PI 3 ) kinase , and Rac are directly opposed by inhibitory signals through SHP 1 . . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| Here we further report that stimulation with GM CSF causes phosphorylation of Syk protein kinase , but neither Casitas B lineage lymphoma ( Cbl ) nor Cbl b in CD 34 ( + ) hemopoietic progenitors can be blocked by anti CD 116 mAb . ^^^ Thus , GM CSF binds to its receptor CD 116 , and consequently activates Syk phosphorylation , which leads to induce CXCR 3 expression . gamma IP 10 and Mig can induce Syk , Cbl , and Cbl b phosphorylation in CD 34 ( + ) progenitors by means of CXCR 3 . gamma IP 10 or Mig has induced neither chemotaxis nor adhesion in GM CSF stimulated Cbl b blocked CD 34 ( + ) hemopoietic progenitors , whereas SDF 1alpha induces both chemotaxis and adhesion in these cells . ^^^ Interestingly , gamma IP 10 and Mig can induce chemotaxis and adhesion in GM CSF stimulated Syk or Cbl blocked CD 34 ( + ) hemopoietic progenitors . ^^^ Thus , Cbl b , but not Syk and Cbl phosphorylation , is essential for gamma IP 10 and Mig induced chemotaxis and adhesion in CD 34 ( + ) hemopoietic progenitors . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| The non receptor tyrosine kinase Syk is a target of Cbl mediated ubiquitylation upon B cell receptor stimulation . ^^^ Whether Cbl ubiquitin ligase activity mediates its negative regulatory effects on cytoplasmic tyrosine kinases of the Syk / ZAP 70 family has not been addressed , nor is it known whether these kinases are regulated via ubiquitylation during lymphocyte B cell receptor engagement . ^^^ Here we show that B cell receptor stimulation in Ramos cells induces the ubiquitylation of Syk tyrosine kinase which is inhibited by a dominant negative mutant of Cbl . ^^^ Intact tyrosine kinase binding and RING finger domains of Cbl were found to be essential for Syk ubiquitylation in 293T cells and for in vitro Syk ubiquitylation . ^^^ These same domains were also essential for Cbl mediated negative regulation of Syk as measured using an NFAT luciferase reporter in a lymphoid cell . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| Engagement of a DAP 12 coupled KIR or Ly 49 results in phosphorylation of DAP 12 , and other key substrates , including the Syk tryosine kinase , phospholipase C , and c Cbl . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| These results suggest that the mechanism of action of SLAP 2 and the related protein SLAP is to promote c Cbl dependent degradation of the tyrosine kinases SYK and ZAP 70 and down regulation of CD 3 at the cell surface . . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| The antigen induced tyrosine phosphorylation of FcepsilonRI , Syk , phospholipase C gamma ( PLC gamma ) , activation of c Jun N terminal kinase ( JNK ) , extracellular signal regulated kinase ( ERK ) , inhibitor of nuclear factor kappaB kinase ( IKK ) , and Ca++ influx were all suppressed in the cells overexpressing Cbl b in the lipid raft . ^^^ These results suggest that Cbl b is a negative regulator of both Lyn Syk LAT and Gab2mediated complementary signaling pathways in FcepsilonRI mediated mast cell activation . . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| Fgr but not Syk tyrosine kinase is a target for beta 2 integrin induced c Cbl mediated ubiquitination in adherent human neutrophils . ^^^ The following observations imply that Syk is such a kinase : ( 1 ) beta ( 2 ) integrins activated Syk in a Src dependent manner , ( 2 ) Syk was associated with Cbl much longer than Fgr was , and ( 3 ) the Syk inhibitor piceatannol ( 3 , 4 , 3 ' , 5 ' tetrahydroxy trans stilbene ) abolished the Cbl associated kinase activity in an in vitro assay . ^^^ Thus Cbl appears to be essential in beta ( 2 ) integrin signalling , first by serving as a matrix for a subsequent agonist induced signalling interaction between Fgr and Syk , and then by mediating ubiquitination of Fgr which possibly affects its interaction with Cbl . . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| This fraction also contained most of the tyrosine phosphorylated proteins including Cbl , Syk and CD 32 itself . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| This phosphorylation was intact in Btk and Cbl deficient DT 40 cells , but was drastically suppressed in Lyn , Syk , or BCAP deficient DT 40 cells . ^^^ Under our conditions , hydrogen peroxide induced PI3K and Akt activation was independent of Lyn , Syk , Cbl , BCAP , or Ras when each was eliminated individually either by mutation or by a specific inhibitor . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| Cbl b negatively regulates B cell antigen receptor signaling in mature B cells through ubiquitination of the tyrosine kinase Syk . ^^^ In B cells from Cbl b deficient mice cross linking the BCRs resulted in sustained phosphorylation of Igalpha , Syk , and phospholipase C ( PLC ) gamma 2 , leading to prolonged Ca2+ mobilization , and increases in extracellular signal regulated kinase ( ERK ) and c Jun NH 2 terminal protein kinase ( JNK ) phosphorylation and surface expression of the activation marker , CD 69 . ^^^ Image analysis following BCR cross linking showed sustained polarization of the BCRs into large signaling active caps associated with phosphorylated Syk in Cbl b deficient B cells in contrast to the BCRs in Cbl b expressing B cells that rapidly proceeded to form small , condensed , signaling inactive caps . ^^^ Significantly , prolonged phosphorylation of Syk correlated with reduced ubiquitination of Syk indicating that Cbl b negatively regulates BCR signaling by targeting Syk for ubiquitination . . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : Recent studies have demonstrated that c Cbl functions as a ubiquitin protein ligase toward immune receptors and non receptor protein tyrosine kinase Syk by facilitating their ubiquitination and subsequent targeting to proteasomes . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| Activation of Epstein Barr virus / C3d receptor ( gp 140 , CR 2 , CD 21 ) on human B lymphoma cell surface triggers Cbl tyrosine phosphorylation , its association with p 85 subunit , Crk L and Syk and its dissociation with Vav . ^^^ First , CD 21 activation triggered Cbl tyrosine phosphorylation , which required c Src kinase but not PI 3 kinase or Syk kinase activities . ^^^ Second , once tyrosine phosphorylated , Cbl interacts with SH 2 domains of p 85 subunit , SH 2 domains of Crk L and with tyrosine phosphorylated Syk kinase . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of several proteins of the GpVI signaling pathway , including the FcR gamma chain , Syk and phospholipase Cgamma 2 ( PLCgamma 2 ) , is increased in the absence of c Cbl . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| ANCA induced phosphorylation of Cbl and intracellular calcium transients , potential downstream mediators of Syk activation , were also blocked by tyrosine kinase inhibitors . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| Interestingly , this domain interacted with Syk at phosphotyrosine 317 , a site phosphorylated in trans by the Src family kinase , Lyn , and identified previously as a binding site for c Cbl . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| Using purified proteins , we studied the ability of Syk , Fyn , and Abl to differentiate between tyrosines in a common PTK substrate , c Cbl . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| Activation is shown to result in c Cbl phosphorylation , and its association with syk and immunoblotting reveals the appearance of a ladder of syk species with molecular weights that are consistent with ubiquitylation of syk . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| The frontier includes characterization of how scaffolding / adapter proteins , such as cbl , gab , grb , p130Cas , and shc , as well as itam containing proteins and nonreceptor tyrosine kinase adapters of the src and syk families , delimit and integrate signals of multiple receptors to bring about specific outcomes . . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| In contrast , FcgammaR mediated tyrosine phosphorylation of Syk , Cbl , or the gamma subunit of FcR was similar in macrophages from wild type and SHPS 1 mutant mice . ^^^ |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13196 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
|