| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.78927922 |
| Crkl is complexed with tyrosine phosphorylated Cbl in Ph positive leukemia . 0.78927922^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.84743569 |
| The SH 2 domain of CRKL can bind to tyrosine phosphorylated proteins such as CBL , HEF 1 , CAS or paxillin . 0.84743569^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.59525994 |
| Here we show that Cbl associates with all three forms of the human Crk protein , predominantly CrkL , following T cell receptor activation of Jurkat T cells . 0.59525994^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.683579 |
| Since CRKL , an SH 2 , SH 3 domain containing adapter protein is known to bind directly to BCR ABL and also binds to tyrosine phosphorylated c CBL , the ability of CRKL to mediate a complex between c CBL and BCR ABL was examined . . 0.683579^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :1.3972816 |
| The adapter protein CrkL was associated with both phosphorylated Cbl and the guanidine nucleotide releasing factor C3G , which catalyzes guanosine triphosphate ( GTP ) exchange on Rap 1 . 1.3972816^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.70876258 |
| Our results show that CrkL associates independently with Cbl or p 85 and suggest that it also participates in larger complexes containing Cbl and p 85 . 0.70876258^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.61868754 |
| The induced association of Crkl with Cbl , Hef 1 , and Fc gamma RI gamma after Fc gamma RI activation and the constitutive association between C3G and Crkl provide the first evidence that a Fc gamma RI gamma Crkl C3G complex may link ITAM receptors to the activation of Rap 1 in myeloid cells . . 0.61868754^^^ Fc gamma RI cross linking of U937IF cells results in the tyrosine phosphorylation of Cbl , Crkl , and Hef 1 , an increase in the association of Crkl with Cbl via direct SH 2 domain interaction and increased Crkl Hef 1 binding . 0.52134361^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :1.0227576 |
| Moreover , in acute leukemia cells , c Cbl was not stably complexed with the tyrosine phosphorylated adaptor protein CrkL . 1.0227576^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :1.2186089 |
| CrkL associated with tyrosine phosphorylated Cbl and itself became tyrosine phosphorylated after Fc gammaRI cross linking . 1.2186089^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.52939802 |
| Crkl binds to BCR / ABL through its N terminal SH 3 domain and is known to interact with several signaling proteins that have been implicated in integrin signaling , including Cbl , Cas , Hef 1 , and paxillin . 0.52939802^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.51064361 |
| The RA dependent interaction of CrkL with CBL was mediated by binding of the SH 2 domain of CrkL to tyrosine phosphorylated CBL , suggesting that CBL provides a docking site for engagement of CrkL in a RA activated cellular pathway . 0.51064361^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.96747735 |
| In addition , we demonstrate that CrkL associates with a large portion of tyrosine phosphorylated Cbl after CD 2 stimulation of NK3 . 3 cells . 0.96747735^^^ In contrast to constitutive Cbl association with Grb 2 , tyrosine phosphorylated Cbl interacts with CrkL via its SH 2 domain only after CD 2 stimulation . 0.63515553^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.5093781 |
| Stimulation with sensitized sheep red blood cells ( sRBCs ) induced tyrosine phosphorylation of CBL and association of CBL with CRKL . 0.5093781^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| The two major sites of cbl tyrosine phosphorylation in abl transformed cells select the crkL SH 2 domain . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the CRKL SH 2 domain has been shown to bind one or more cellular proteins , one of which is p 120 ( CBL ) . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| EGF stimulation also induced the association of Cbl with Src homology and collagen ( Shc ) protein , p 85 subunit of the phosphatidylinositol 3 kinase and Crk proteins , in particular with the CrkL isoform . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| Steel factor induces tyrosine phosphorylation of CRKL and binding of CRKL to a complex containing c kit , phosphatidylinositol 3 kinase , and p 120 ( CBL ) . ^^^ We conclude that SF induces the formation of a signaling complex potentially containing CRKL and p 120 ( CBL ) , both of which bind to c Kit through p 85 ( PI3K ) . ^^^ These data suggest that one function of CRKL in normal cells might be to recruit signaling molecules such as CBL into a complex with PI3K . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| Recent studies have shown that the CrkL adaptor protein and the Cbl protooncoprotein are constitutively tyrosine phosphorylated and form a preformed complex in cells expressing Bcr abl . ^^^ Of the three Crk isoforms expressed in hematopoietic cells ( CrkL , CrkII , and CrkI ) , tyrosine phosphorylated Cbl binds preferentially to CrkL and CrkII . ^^^ The amount of Cbl associated with CrkL and CrkII exceeded the fraction of Cbl associated with Grb 2 indicating that unlike other receptor systems , the Cbl Crk association represents the dominant complex of Cbl in growth factor stimulated hematopoietic cells . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| Differential signaling after beta 1 integrin ligation is mediated through binding of CRKL to p 120 ( CBL ) and p 110 ( HEF 1 ) . ^^^ The major tyrosine phosphorylated CRKL binding protein in the megakaryocytic MO7e cells was identified as p 120 ( CBL ) , the cellular homolog of the 5 Cbl oncoprotein . ^^^ Thus , in different cell types CRKL and its SH 3 associated proteins may form different multimeric complexes depending on whether p 120 ( CBL ) or p 110 ( HEF 1 ) is tyrosine phosphorylated after integrin ligation . ^^^ The shift in association of CRKL and its SH 3 associated proteins from p 120 ( CBL ) to p 110 ( HEF 1 ) could contribute to different functional outcomes of `` outside in ' ' integrin signaling in different cells . . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| The major target proteins have been identified , and are very similar to the known substrates of BCR / ABL , including Shc , CBL , CRKL , and several proteins in the cytoskeleton . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| Erythropoietin and IL 3 induce tyrosine phosphorylation of CrkL and its association with Shc , SHP 2 , and Cbl in hematopoietic cells . ^^^ The present study demonstrates that erythropoietin ( Epo ) and IL 3 induce tyrosine phosphorylation of the SH2 / SH3 containing adapter protein CrkL and its transient association with tyrosine phosphorylated SHP 2 , Shc , and Cbl in a murine IL 3 dependent cell line , 32D , expressing the Epo receptor ( EpoR ) . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| The proto oncoprotein p 120 ( Cbl ) and Hef 1 , but not p 130 ( Cas ) , were detectably phosphorylated on tyrosine in P190Bcr / Abl expressing leukemic cells and were found in complex with Crkl , showing the existence of protein complexes in P190Bcr / Abl leukemic cells , consisting of P190Bcr / Abl , Crkl , and Hef 1 or p 120 ( Cbl ) . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| CBL interactions with other phosphoproteins , among them SHC and CRKL , were also augmented in response to pretreatment with the PTPase inhibitors . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| Glutathione S transferase fusion protein data demonstrate that the constitutive interaction of CBL with GRB 2 and CRKL is mediated via two discrete regions of the CBL C terminus . ^^^ The distal C terminus of CBL ( residues 671 906 ) binds the CRKL adapter protein . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| Crkl , an SH2 / SH3 adapter protein , becomes coimmunoprecipitated specifically with STAT 5 from erythropoietin stimulated erythroid cells ; although it was shown to become associated with c Cbl in the studies using cell lines . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| When phosphorylated , Crkl binds through its Src homology 2 ( SH 2 ) domain to other tyrosine phosphoproteins such as paxillin and Cbl . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| The alphaCbl immunoprecipitations revealed constitutive binding of Nck and Grb 2 to Cbl and FcgammaRII inducible binding of CrkL to Cbl . ^^^ The interactions of Cbl with Nck and CrkL were phosphorylation dependent since dephosphorylation of cellular proteins with potato acid phosphatase abrogated binding . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| Upon activation , tyrosine phosphorylated Cbl interacts with the Src homology 2 ( SH 2 ) domains of several signaling proteins , e . g . , phosphatidylinositol 3 kinase ( PI 3 K ) and CrkL . ^^^ In the present study , we report that pretreatment of Jurkat T cells with PMA reduced the anti CD 3 induced tyrosine phosphorylation of Cbl and , consequently , its activation dependent association with PI 3 K and CrkL . ^^^ A specific protein kinase C ( PKC ) inhibitor ( GF 109203X ) reversed the effect of PMA on tyrosine phosphorylation of Cbl and restored the activation dependent association of Cbl with PI 3 K and CrkL . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| Analysis of the role of individual tyrosine phosphorylation sites of c Cbl in these phenomena indicates that both phosphatidylinositol 3 ' kinase and the CrkL adaptor protein may be involved in the observed effects of c Cbl . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| Here we show that this attenuation of tyrosine phosphorylation of Shc , CrkL , and the proto oncogene Cbl is mimicked by treatment of mouse T lymphocytes or cultured Jurkat cells with phorbol 12 myristate 13 acetate . ^^^ Activation of protein kinase C by phorbol ester also causes rapid ( t ( 1 ) / ( 2 ) = 2 min ) dissociation of both CrkL and p85 / phosphoinositide 3 kinase from Cbl concomitant with Cbl tyrosine dephosphorylation . ^^^ Furthermore , the rate of attenuation of both Cbl tyrosine phosphorylation and its association with CrkL following stimulation with anti CD3 / CD4 antibodies is much slower in Jurkat cells treated with GF109203X . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| Our results provide the first evidence that FcalphaR mediated signals modulate a multimolecular adaptor protein complex containing Grb 2 , Shc , SHIP , CrkL , Cbl , and SLP 76 . ^^^ These data indicate that the stimulation of FcalphaR results in the modulation of adaptor complexes containing tyrosine phosphorylated Cbl , Shc , SHIP , Grb 2 , and Crkl . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| We report that IFN gamma activates a distinct signaling cascade involving the c cbl protooncogene product , CrkL adapter , and small G protein Rap 1 . ^^^ During treatment of NB 4 human cells with IFN gamma , c cbl protooncogene product is rapidly phosphorylated on tyrosine and provides a docking site for the src homology 2 domain of CrkL , which also undergoes IFN gamma dependent tyrosine phosphorylation . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| Rap 1 was transiently activated following TCR stimulation of both total thymocytes and purified double positive thymocytes , and this activation correlated with tyrosine phosphorylation of Cbl and Cbl association with CrkL . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| In this study , we show that the adapter proteins CrkL and Cbl undergo increases in tyrosine phosphorylation and form an intracellular complex in platelets stimulated with the snake venom toxin convulxin , a selective agonist at the collagen receptor glycoprotein 6 ( GPVI ) . ^^^ This was confirmed in the present study , and shown to result in a weak constitutive association of CrkL with Cbl and a number of other unidentified tyrosine phosphorylated proteins . ^^^ There was no further increase in phosphorylation of CrkL in CML platelets in response to GPVI activation , whereas phosphorylation of Cbl and its association with CrkL were potentiated . ^^^ Despite the minimal effect on platelet activation in CML patients , we can not exclude a role for CrkL or Cbl in signal transduction pathways stimulated via GPVI . . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| L selectin tyrosine phosphorylates cbl and induces association of tyrosine phosphorylated cbl with crkl and grb 2 . ^^^ There is an activation induced association of tyrosine phosphorylated Cbl with Grb 2 and CrkL , respectively , but not CrkII . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| We previously demonstrated that , in Epo stimulated cells , an adapter protein , CrkL , becomes tyrosine phosphorylated , physically associates with Shc , SHP 2 , and Cbl , and plays a role in activation of the Ras / Erk signaling pathway . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| CD 2 crosslinking markedly induced tyrosine phosphorylation of Cbl associated with Grb 2 or CrkL , Shc and LAT , compared with IL 2 stimulation . ^^^ However , costimulation of IL 2 with CD 2 crosslinking remarkably enhanced associations of Grb 2 Shc and CrkL Cbl , compared to IL 2 stimulation or CD 2 crosslinking alone . ^^^ In vitro binding studies using GST fusion proteins revealed that interactions of Grb 2 Shc and CrkL Cbl were mediated through each SH 2 domain in tyrosine phosphorylation dependent manner . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| CBL was predominantly in a complex with phosphatidylinositol 3 ' kinase and CRKL , while CBL B was not associated with any significant phosphatidylinositol 3 ' kinase activity . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| In this study , we demonstrate that HGF induces strong tyrosine phosphorylation of the proto oncogene product c Cbl in B cells and increases Cbl association with the Src family tyrosine kinases Fyn and Lyn , as well as with phosphatidylinositol 3 kinase and CrkL . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| We demonstrate here that cross linking of alpha4beta1 by anti alpha 4 antibody induces tyrosine phosphorylation of Pyk 2 , Shc , and Cbl as well as binding of the adaptor protein CrkL with Cbl in a murine hematopoietic cell line , 32D / EpoR Wt . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| Previous studies have proposed that Cbl is also a positive regulator of CrkL C3G signalling , which leads to Rap 1 activation . ^^^ Cbl was shown to promote the ubiquitylation of CrkL without any apparent effect on its stability . ^^^ Remarkably , the membrane translocation of C3G , its association with CrkL , and the guanine nucleotide exchange activity of C3G were all increased in Cbl ( / ) thymocytes . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| Lyp was localized to the cytosol , and overexpression of Lyp caused reduction in the phosphorylation levels of multiple proteins in KCL 22 chronic myeloid leukemia blast cells including Cbl , Bcr Abl , Erk1 / 2 , and CrkL . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| Imatinib mesylate remarkably reduced tyrosine phosphorylation of Bcr Abl , Cbl , and Crkl in a time dependent manner , and their complex formation also was affected . ^^^ |
|
| Interacting proteins: P46109 and P22681 |
Pubmed |
SVM Score :0.0 |
| We found that each kinase produced a distinct pattern of c Cbl phosphorylation , which altered the phosphotyrosine dependent interactions between c Cbl and CrkL or phosphatidylinositol 3 ' kinase ( PI 3 K ) . ^^^ |
|