| Interacting proteins: P48378 and P22670 |
Pubmed |
SVM Score :0.99398635 |
| In this report , we demonstrate that RFX 1 forms homodimers as well as heterodimers with RFX 2 spanning the collagen transcription start site . 0.99398635^^^ |
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| Interacting proteins: P48378 and P22670 |
Pubmed |
SVM Score :0.0 |
| We termed this protein Drosophila RFX 2 ( dRFX 2 ) and this element dRFX 2 site . ^^^ |
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| Interacting proteins: P48378 and P22670 |
Pubmed |
SVM Score :0.0 |
| The genes for MHC class 2 regulatory factors RFX 1 and RFX 2 are located on the short arm of chromosome 19 . ^^^ RFX 2 is a structurally very similar protein with identical DNA binding features . ^^^ With the use of biotinylated genomic cosmid clones specific for RFX 1 and RFX 2 , respectively , it was then possible to localize RFX 1 at 19p13 . 1 and RFX 2 at 19p13 . 2 p13 . 3 . ^^^ |
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| Interacting proteins: P48378 and P22670 |
Pubmed |
SVM Score :0.0 |
| We show here that RFX 1 belongs to a previously unidentified family of DNA binding proteins of which we have cloned three members , RFX 1 , RFX 2 , and RFX 3 , from humans and mice . ^^^ They have very similar DNA binding specificities and heterodimerize both in vitro and in vivo . mRNA levels for all three genes , particularly RFX 2 , are elevated in testis . ^^^ In other cell lines and tissues , RFX mRNA levels are variable , particularly for RFX 2 and RFX 3 . ^^^ |
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| Interacting proteins: P48378 and P22670 |
Pubmed |
SVM Score :0.0 |
| Locations of human and mouse genes encoding the RFX 1 and RFX 2 transcription factor proteins . ^^^ The human RFX 1 and RFX 2 genes have been assigned by in situ hybridization to chromosome 19p13 . 1 and 19p13 . 3 , respectively . ^^^ In this paper , we present data that localize RFX 1 and RFX 2 precisely within the detailed physical map of human chromosome 19 and genetic data that assign Rfx 1 and Rfx 2 to homologous regions of mouse chromosomes 8 and 17 , respectively . ^^^ |
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| Interacting proteins: P48378 and P22670 |
Pubmed |
SVM Score :0.0 |
| Here , we demonstrate by yeast one hybrid screening that RFX 2 protein specifically binds to this cis element . ^^^ RFX 2 belongs to the RFX DNA binding protein family , the biological role of which remains obscure . ^^^ Using an electrophoretic mobility shift assay , we further show that RFX 1 , RFX 2 , and RFX 3 homodimers and heterodimers specifically bind to the cis element of the IL 5Ralpha promoter . ^^^ The mRNA expression of RFX 1 , RFX 2 , and RFX 3 was detected ubiquitously , but in transient transfection assays , multimerized RFX binding sites in front of a basal promoter efficiently functioned in a tissue and lineage specific manner . ^^^ |
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| Interacting proteins: P48378 and P22670 |
Pubmed |
SVM Score :0.0 |
| RFX 4 contains evolutionarily conserved regions , including a RFX type DNA binding domain , a dimerization domain , and other conserved regions , and is closely related to RFX 1 , RFX 2 , and RFX 3 in structure . ^^^ Immunoprecipitation analyses showed that RFX 4 interacts physically with RFX 2 , RFX 3 , and RFX 4 itself but not with RFX 1 . ^^^ RFX 4 contains evolutionarily conserved regions , including a RFX type DNA binding domain , a dimerization domain , and other conserved regions , and is closely related to RFX 1 , RFX 2 , and RFX 3 in structure . ^^^ |
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| Interacting proteins: P48378 and P22670 |
Pubmed |
SVM Score :0.0 |
| Regulatory factor X 2 ( RFX 2 ) binds to the H1t / TE1 promoter element and activates transcription of the testis specific histone H1t gene . ^^^ We purified an 85 kDa protein from rat testis nuclear extracts using the TE 1 subelement as an affinity chromatography probe and analysis revealed that the protein was RFX 2 . ^^^ Polyclonal antibodies directed against RFX 2 supershift the low mobility testis nuclear protein complex formed in electrophoretic mobility shift assays ( EMSA ) . ^^^ RFX 2 derived from primary spermatocytes , where the transcription factor is relatively abundant , binds with high affinity to the TE 1 element . ^^^ Coexpression of RFX 2 together with an H1t promoter / reporter vector activates the H1t promoter in a cultured GC 2spd germinal cell line , but mutation of either the TE 1 subelement or the TE 2 subelements represses activity . ^^^ |
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| Interacting proteins: P48378 and P22670 |
Pubmed |
SVM Score :0.0 |
| We showed that in vitro translated RFX 3 , as well as RFX 1 and RFX 2 , was able to bind B10 . ^^^ |
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| Interacting proteins: P48378 and P22670 |
Pubmed |
SVM Score :0.0 |
| RFX 2 is a potential transcriptional regulatory factor for histone H1t and other genes expressed during the meiotic phase of spermatogenesis . ^^^ The testis specific binding complex contains RFX 2 , probably as a homodimer . ^^^ Western blots confirmed that RFX 2 expression is greatly enhanced in adult testis and that RFX 2 is equally prominent in highly enriched populations of late pachytene spermatocytes and round spermatids . ^^^ Immunohistochemistry carried out on mouse testis showed that RFX 2 is strongly expressed in pachytene spermatocytes , remains high in early round spermatids , and declines only in advance of nuclear condensation . ^^^ RFX specific band complexes were also identified for both the mouse lamin C 2 and Sgy promoters , using either testis nuclear extracts or in vitro synthesized RFX 2 . ^^^ |
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| Interacting proteins: P48378 and P22670 |
Pubmed |
SVM Score :0.0 |
| Transcriptional activation of the testis specific histone H1t gene by RFX 2 may require both proximal promoter 10 box elements . ^^^ RFX 2 is the major enriched protein derived from rat testis nuclear extracts when using the TE 1 element as an affinity chromatography probe . ^^^ Co expression of RFX 2 together with an H1t promoted reporter vector in transient expression assays activates the H1t promoter in the GC 2spd germinal cell line , and mutation of either 10 box significantly represses activity . ^^^ However , RFX 2 partially reactivates the promoter when either of the 10 box elements is independently mutated . ^^^ In order to totally block reactivation by RFX 2 , it is necessary to mutate both 10 boxes simultaneously . ^^^ |
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| Interacting proteins: P48378 and P22670 |
Pubmed |
SVM Score :0.0 |
| NA |
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