| Interacting proteins: P21754 and P09958 |
Pubmed |
SVM Score :0.0 |
| Secretion of mouse ZP 3 , the sperm receptor , requires cleavage of its polypeptide at a consensus furin cleavage site . ^^^ |
|
| Interacting proteins: P21754 and P09958 |
Pubmed |
SVM Score :0.0 |
| The canonical furin proteolytic processing signal at the C terminus was found in all the ZP 3 polypeptide sequences except of carp and frog . ^^^ |
|
| Interacting proteins: P21754 and P09958 |
Pubmed |
SVM Score :0.0 |
| Like human and rabbit ZP 1 an altered furin cleavage site upstream of the C terminal hydrophobic domain also occurs in possum ZP 3 ( S R K R ) , suggestive of processing by a furin related endoprotease . ^^^ |
|
| Interacting proteins: P21754 and P09958 |
Pubmed |
SVM Score :0.0 |
| Conserved furin cleavage site not essential for secretion and integration of ZP 3 into the extracellular egg coat of transgenic mice . ^^^ However , mutation of the furin site ( RNRR > ANAA ) does not affect the intracellular trafficking or secretion of an enhanced green fluorescent protein ( EGFP ) ZP 3 fusion protein in heterologous somatic cells . ^^^ These in vitro results are confirmed in transgenic mice expressing EGFP ZP 3 with or without the mutant furin site . ^^^ |
|
| Interacting proteins: P21754 and P09958 |
Pubmed |
SVM Score :0.0 |
| The mature C termini of ZP 1 and ZP 3 end two amino acids upstream of a conserved dibasic residue that is part of , but distinct from , the consensus furin cleavage sequence , while the C terminus of ZP 2 was not determined . ^^^ |
|
| Interacting proteins: P21754 and P09958 |
Pubmed |
SVM Score :0.0 |
| We have previously reported that the newly synthesized ZPC ( proZPC ) in the granulosa cells is cleaved at the consensus furin cleavage site to generate mature ZPC prior to secretion . ^^^ |
|
| Interacting proteins: P21754 and P09958 |
Pubmed |
SVM Score :0.0 |
| Carboxy terminal proteolytic processing at a consensus furin cleavage site is a prerequisite event for quail ZPC secretion . ^^^ We have previously reported that the newly synthesized ZPC ( proZPC ) in granulosa cells is cleaved at a consensus furin cleavage site to generate mature ZPC prior to secretion . ^^^ In the present study , we examined the effect of the proteolytic cleavage of proZPC on ZPC secretion by using a specific inhibitor of furin endoprotease and site directed mutagenesis of the furin cleavage site . ^^^ Western blot analysis demonstrated that the furin inhibitor efficiently blocked both the proteolytic cleavage of proZPC and the subsequent ZPC secretion . ^^^ Therefore , the C terminal proteolytic processing of proZPC at the consensus furin cleavage site is a prerequisite event for quail ZPC secretion . . ^^^ |
|
| Interacting proteins: P21754 and P09958 |
Pubmed |
SVM Score :0.0 |
| These results suggest that ZPB is processed at its furin consensus site , whereas ZPC is processed N terminal to the furin consensus site . ^^^ |
|
| Interacting proteins: P21754 and P09958 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21754 and P09958 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21754 and P09958 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21754 and P09958 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21754 and P09958 |
Pubmed |
SVM Score :0.0 |
| NA |
|