Pubmed abstracts for Protein-Protein Interaction search result :


Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
The activator NTF 1 , which binds TAFII 150 , stimulates transcription with a complex containing only TBP , TAFII 250 , and TAFII 150 , whereas Sp 1 binds and additionally requires TAFII 110 for activation . ^^^
Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
The oestrogen receptor stimulates transcription by means of at least two distinct transcriptional activation domains , TAF 1 in the N terminal domain and TAF 2 in the hormone binding domain . ^^^
Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
We demonstrate that the individual hER transactivation functions , TAF 1 and TAF 2 , are both functional in yeast , and that the hormone dependent TAF 2 is the more potent activator on our reporters . ^^^
Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
These same alterations in chromatin structure were induced by a truncated estrogen receptor with both TAF 1 and TAF 2 transactivation functions deleted , suggesting that DNA binding per se disrupts chromatin structure . ^^^
Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
Our results indicate that both TAF 2 and the N terminal transactivation region ( TAF 1 ) are functional in yeast and contribute synergistically to the receptor ' s total activity . ^^^ Furthermore , we show that in yeast the antagonistic effects of the antiestrogen nafoxidine arise from a modulation of the synergistic interactions of TAF 1 and TAF 2 , and not simply from an inactivation of TAF 2 by antihormone . ^^^
Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
Both hPR TAF 1 and TAF 2 activate a minimal promoter in yeast . ^^^
Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
We have previously demonstrated that the human oestrogen receptor ( hER ) contains two transcriptional activation functions located in the N terminal region ( TAF 1 ) and in the hormone binding domain ( TAF 2 ) , which can act both independently and synergistically in a promoter and cell specific manner in animal cells . ^^^ We have also demonstrated that hER can activate transcription from chimaeric oestrogen responsive GAL 1 promoters in yeast , and shown that transcriptional activation was due to TAF 1 , whereas TAF 2 showed little , if any , transcriptional activity on these promoters . ^^^ By using a more complex promoter derived from the URA 3 gene , we now show that TAF 2 is also active in yeast , and that the activities of TAF 1 and TAF 2 are promoter context specific in yeast . ^^^
Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
Various oestrogen responsive reporter genes and vectors expressing truncated or chimeric human oestrogen receptors ( hER ) containing either of the two independent hER transcriptional activation functions ( TAF 1 and TAF 2 ) have been transfected into HeLa cells , chicken embryo fibroblast ( CEF ) or yeast cells to investigate the agonistic activity of the anti oestrogen 4 hydroxytamoxifen ( OHT ) . ^^^
Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
Activation of the c fos gene promoter in HeLa cells requires the DNA binding domain of the estrogen receptor , and can be achieved independently by the TAF 1 and the TAF 2 transcriptional activation functions of this molecule . ^^^
Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
We have previously shown that the two transcriptional activation functions ( TAF 1 and TAF 2 ) of the human estrogen receptor ( hER ) have synergistic properties different from one another and from those of acidic activating domains ( AADs ) . ^^^
Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
The analysis of a series of deletion mutants and chimeric receptors revealed the presence of two transcription activation functions ( TAFs ) , located in the N terminal region A / B ( TAF 1 ) and the hormone binding domain ( TAF 2 ) . ^^^ In the presence of RU 486 TAF 2 was inactive , while TAF 1 within the hPR form B / RU486 complex activated transcription from a reporter gene containing a single palindromic PRE . ^^^
Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
By analyzing the ability of TAF 1 , TAF 2 , and the GAL 4 and VP 16 acidic activating domains ( AADs ) to homosynergize and heterosynergize with one another and with the factor binding to the upstream element ( UE ) of the adenovirus 2 major late promoter , we show that the activation properties of TAF 1 and TAF 2 are different and distinct from those of AADs , in agreement with the absence of acidic amino acid stretches in TAF 1 and TAF 2 . . ^^^
Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
These sequences , transactivation function 1 ( TAF 1 ) in the amino terminus and TAF 2 at the carboxyl terminus , display distinct transcriptional functions . ^^^ Using specific receptor mutations it has been shown that on some promoters both TAF 1 and TAF 2 are required for maximal transcriptional activity , whereas on others , additional factors bound to the target promoter can functionally substitute for TAF 1 or TAF 2 . ^^^ Estrogen functions as an ER agonist by promoting functional synergism between TAF 1 and TAF 2 . ^^^ These data indicate that alterations in the cellular expression of proteins which mimic TAF 1 or TAF 2 activity can have a profound effect on the pharmacology of ER modulators . ^^^
Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
With mutated receptors we were able to show that this activity was mainly due to TAF 1 whereas TAF 2 remained silent . ^^^
Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
Like 17 beta estradiol , OP is capable of stimulating the activity of both transcriptional activation functions , TAF 1 and TAF 2 , in the receptor , as judged by analyzing the activity of the wild type and mutant receptors in transiently transfected cells . ^^^
Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
We have used a series of human estrogen receptor ( ER ) mutants to evaluate the cell and promoter specific transcriptional activities of the TAF 1 and TAF 2 transactivation regions within the human ER . ^^^ We show that the manifestation of TAF 1 or TAF 2 function depends strongly upon promoter context ; on certain promoters , both the TAF 1 and TAF 2 activators are required for wild type transcriptional activity , whereas on other promoters , the TAF 1 and TAF 2 activators function independently . ^^^ These data suggest that 1 ) TAF 1 may be the major transcriptional activator of the ER ; and 2 ) TAF 2 functions as a transcriptional facilitator . ^^^ On promoters where TAF 2 function is provided independently of the ER , the TAF 1 function of the ER can function independently of TAF 2 activity , allowing triphenylethylene derived antiestrogens to demonstrate partial agonist activity . ^^^
Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
The presence of two TAFs was confirmed by cotransfection of RAR beta deletion constructs with the human RAR beta 2 promoter as reporter , showing that the absence of RAR beta TAF 1 causes a decrease in transactivation , whereas truncation of TAF 2 completely blocks this function . ^^^
Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
The estrogen receptor ( ER ) contains two nonacidic transcriptional activation functions , AF 1 and AF 2 ( formerly TAF 1 and TAF 2 ) . ^^^
Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
Analysis of PR deletion mutants indicated that both transcriptional activation domains of PR ( TAF 1 and TAF 2 ) were involved in the inhibition of IGFBP 1 expression . ^^^
Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
Different functional domains of TAFII 250 modulate expression of distinct subsets of mammalian genes . ^^^
Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
TAFII 55 binding to TAFII 250 inhibits its acetyltransferase activity . ^^^
Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
Total TAFI antigen ( including pro TAFI , TAFI and the inactive form of TAFI [ TAFIi ] ) and pro TAFI were determined in 17 patients with haemophilia A . ^^^
Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
Total TAFI antigen [ i . e . pro TAFI , TAFI and inactive carboxypeptidase U ( TAFIi ) ] tended to decrease in both the patient groups ( 59 . 7 + / 7 . 2 and 73 . 4 + / 8 . 9 % with and without microvascular complications , respectively ) compared with controls ( 91 . 9 + / 12 . 2 % ) ( P = 0 . 12 ) . ^^^
Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
TAFI spontaneously becomes inactive as a result of a temperature dependent conformational change in the protein ( TAFIi ) . ^^^ In this study , pro TAFI , total TAFI antigen and TAFI TAFIi antigen levels were measured in 32 patients with hemophilia A , 4 patients with hemophilia B , 21 patients with von Willebrand disease ( VWD ) and 13 healthy controls . ^^^ A statistically significant increase in TAFI TAFIi antigen was found in hemophilia A ( 1 . 05+ / 1 . 01 mg / L ) ( p < 0 . 05 ) and in VWD patients ( 0 . 96+ / 1 . 01 mg / L ) ( p < 0 . 05 ) compared to controls ( 0 . 55+ / 0 . 36 mg / L ) . ^^^ Neither did pro TAFI nor TAFI TAFIi levels differ within the group of hemophilia A patients in relation to severity ( mild , moderate and severe ) or among the VWD patients in relation to subtype ( type 1 , type 2A and type 3 ) . ^^^ These findings indicate an increased conversion of pro TAFI to TAFI and / or TAFIi in patients with bleeding disorders . ^^^
Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
Within TFIID , the putative orthologs of TAF 1 , TAF 2 , TAF 7 and TAF 10 were also predicted . ^^^
Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
We identified host acidic proteins , template activating factor 1 ( TAF 1 ) , TAF 2 , and TAF 3 as stimulatory factors for replication from the adenovirus DNA protein 7 complex . ^^^
Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
Our analysis also indicates that TAF 5 , TAF 6 , TAF 9 , and TAF 12 play key roles in stability of the complex , whereas TBP , TAF 1 , TAF 2 , and TAF 11 contribute very little to complex stability . ^^^ Based on our results , we propose that holo TFIID comprises a stable core subcomplex containing TAF 4 , TAF 5 , TAF 6 , TAF 9 , and TAF 12 decorated with peripheral subunits TAF 1 , TAF 2 , TAF 11 , and TBP . ^^^
Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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Interacting proteins: Q6P1X5 and P21675 Pubmed SVM Score :0.0
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