| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.7106104 |
| The TAF N terminal domain ( TAND ) of TAF 1 has been shown to interact with TBP and to modulate the interaction of TBP with the TATA box , which is required for transcriptional initiation and activation of TATA promoter operated genes . 0.7106104^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.6580777 |
| Based on our results , we propose that holo TFIID comprises a stable core subcomplex containing TAF 4 , TAF 5 , TAF 6 , TAF 9 , and TAF 12 decorated with peripheral subunits TAF 1 , TAF 2 , TAF 11 , and TBP . 0.6580777^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| Cloning and expression of human TAFII 250 : a TBP associated factor implicated in cell cycle regulation . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| Furthermore , TFIIH and TAFII 250 , the largest subunit of TFIID , can both phosphorylate PC 4 . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| Recently , TAFII 250 , TATA box binding protein associated factor , was suggested to be involved in dystonia parkinsonism . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| The p 250 subunit of native TATA box binding factor TFIID is the cell cycle regulatory protein CCG 1 . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| TBP recruitment was compromised in two different mot 1 mutant strains , but was only moderately affected in a taf 1 Ts strain . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| TBP was found in the linker domain between lobes A and C in a way that the N terminal 100 residues of TAF 1 are spanned over it . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| The N terminal domain ( TAND ) of TAF 1 protein ( Taf1p ) inhibits TBP by binding to its concave and convex surfaces . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| Both Taf 1 and Taf 5 are ubiquitinated , and the ubiquitination pattern of TFIID changes when BUL 1 or BRE 5 is deleted . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| Representatives of the TFIID pathway include the TATA binding protein , TAF 1 , and Bdf 1 . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| Within TFIID , the putative orthologs of TAF 1 , TAF 2 , TAF 7 and TAF 10 were also predicted . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| Resonance assignments of 30 kDa complexes of TFIID subunit TAF 1 with TATA binding protein . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| Coimmunoprecipitation assays showed that TAFII 80 interacted with TAFII 250 , TAFII 31 , TAFII 20 , and TBP , but not with TAFII 55 . ^^^ The N terminus of TAFII 80 ( residues 1 100 ) interacts with both TAFII 31 and TAFII 20 , while two C terminal regions are involved , respectively , in interactions with TAFII 250 and TFIIF alpha ( RAP 74 ) ( residues 203 276 ) and with TBP and TFIIE alpha ( residues 377 505 ) . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| When TBP was bound first by either TAFI 110 , 63 , or 48 , subunits of TFIID such as TAFII 250 and 150 did not bind TBP . ^^^ Conversely , if TBP first formed a complex with TAFII 250 or 150 , the subunits of SL 1 did not bind TBP . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| Furthermore , a partial TFIID complex containing Drosophila TATA binding protein ( dTBP ) , human TAFII 250 , dTAFII 60 , and dTAFII 40 supported activation by a Gal 4 p53 fusion protein in vitro , whereas TBP or a subcomplex lacking TAFII 40 and TAFII 60 did not . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| A minimal complex containing TBP and TAFII 250 directs basal but not activator responsive transcription . ^^^ The activator NTF 1 , which binds TAFII 150 , stimulates transcription with a complex containing only TBP , TAFII 250 , and TAFII 150 , whereas Sp 1 binds and additionally requires TAFII 110 for activation . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| Recombinant TAFII60 / 70 binds weakly to TBP and tightly to the largest subunit of TFIID , TAFII 250 . ^^^ In the presence of TAFII60 / 70 , TBP and TAFII 250 , a stable ternary complex is formed . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| The TAFII 250 subunit of the human transcription factor IID ( TFIID ) rescues the temperature sensitive hamster cell line ts 13 and overcomes a G 1 arrest . ^^^ This transcriptional defect can be rescued by purified human TFIID or by expression of wild type TAFII 250 in ts 13 cells . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| Here we report the cloning of Drosophila TAFII 250 and the assembly of a partial complex containing recombinant TBP , TAFII 110 and the C terminal domain of TAFII 250 . ^^^ This triple complex supports Sp 1 activation and reveals specific interactions between TAFII 250 , TBP and TAFII110 . . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| A quadruple complex containing TATA binding protein ( TBP ) , TAFII 250 , TAFII 110 , and TAFII 60 mediated transcriptional synergism by BCD and HB , whereas triple TBP TAFII complexes lacking one or the other target coactivator failed to support synergistic activation . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| Mutations in TBP affect the synthesis of short and full length transcripts as well as Tat activation similarly , and these effects correlate with the previously described effects of these mutations on binding of TBP to the TBP associated factor TAFII 250 in vitro . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| A TBP mutant defective in interaction with TBP associated factor TAFII 250 also failed to mediate transactivation through the CIITA activation domain . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| A specific mutation in TAFII 250 , the largest subunit of the transcription factor TFIID , disrupts cell growth control in the temperature sensitive mutant hamster cell line ts 13 . ^^^ These results suggest that the ts 13 mutation of TAFII 250 has compromised the ability of TFIID to mediate activation of transcription by specific enhancer factors such as ATF , as well as to perform certain core promoter functions . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| X , the HBV encoded coactivator , suppresses the phenotypes of TBP and TAFII 250 mutants . ^^^ Collectively , our results suggest that pX suppresses some of the phenotypes of TBP and TAFII 250 mutations , implying that pX circumvents the need for a holo TFIID complex for transcription activation to proceed . . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| The C terminal portion ( amino acids 416 to 550 ) of the p 65 subunit of NF kappaB is a potent activator when assayed as a Gal fusion in the reconstituted transcription system and interacts both with TATA binding protein ( TBP ) and with several human TBP associated factors ( TAFs ) that include TAFII 250 . ^^^ The p 65 activation domain mediates transcription activation in the presence of partially reconstituted TFIID species that include a minimal complex containing only TBP and TAFII 250 . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| Recombinant TAFII 250 inhibits TBP DNA binding , whereas preincubation of TFIIA with TBP prevents this inhibition . ^^^ Thus , TFIIA competes with TAFII 250 for access to TBP and alters the TATA binding properties of the resulting complex . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| The largest subunit of the human transcription factor TFIID , TAFII 250 , was previously reported to contain serine / threonine kinase domains that can autophosphorylate and transphosphorylate the large subunit of the basal factor TFIIF . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| We now show that repression results from the interaction of Tat with the TAFII 250 component of the general transcription factor , TFIID . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| Here , it is shown that TAFII 250 , the largest subunit of TFIID , contains two tandem bromodomain modules that bind selectively to multiply acetylated histone H 4 peptides . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| A biochemical approach identified the Drosophila coactivator TAFII 250 , the central subunit within the general transcription factor TFIID , as a histone specific ubiquitin activating / conjugating enzyme ( ubac ) . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| Chromatin immunoprecipitation analyses demonstrated that T cell activation triggers in vivo binding of endogenous c Rel , c Jun , C / EBP beta , and HMG IC to the bfl 1 regulatory region , coincident with selective recruitment of coactivators TAFII 250 and p 300 , SWI / SNF chromatin remodeling factor component BRG 1 , and basal transcription factors TATA binding protein ( TBP ) and TFIIB , as well as hyperacetylation of histones H 3 and H 4 . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| CCG1ME bound to TBP , creating its own TFIID complex different from that of the endogenous mutated CCG 1 in ts+ transformants of tsBN 462 cells . . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| The ts 13 mutation in the TAF ( 2 ) 250 subunit ( CCG 1 ) of TFIID directly affects transcription of D type cyclin genes in cells arrested in G 1 at the nonpermissive temperature . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| The retinoblastoma tumor suppressor gene product ( Rb ) binds directly to the largest TFIID subunit , TATA binding protein associated factor TAF ( 2 ) 250 , first identified as the cell cycle regulatory protein CCG 1 . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| The TATA binding protein associated factor TAF ( 2 ) 250 , the largest subunit of TFIID , was first identified as the cell cycle regulatory protein CCG 1 . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : CIA , an interactor of the CCG 1 histone acetyltransferase subunit of TFIID , was identified as a human histone chaperone . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| The temperature sensitive cell line ts 13 is mutated in CCG 1 , the gene encoding TAF ( 2 ) 250 , the largest of the TATA binding protein associated factors ( TAFs ) in TFIID . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| Yeast Cia1p is a homologue of human CIA ( CCG 1 interacting factor A ) , which possesses nucleosome assembly activity and interacts with the human TFIID subunit CCG 1 and the C terminal domain of histone H 3 . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| The TAND domain of TAF 1 plays a primary inhibitory role at very few genes , but its function becomes widespread when other TBP interactions are compromised . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| Our finding that recruitment of TFIID , by insertion of a TBP binding site upstream of the TDE 1 , restores basal but not activated transcription supports the model that TAF 1 carries out two independent functions at the cyclin D 1 promoter . . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| Mutations in the histone fold domain of the TAF 12 gene show synthetic lethality with the TAF 1 gene lacking the TAF N terminal domain ( TAND ) by different mechanisms from those in the SPT 15 gene encoding the TATA box binding protein ( TBP ) . ^^^ Although it is well known that the TAF N terminal domain ( TAND ) at the amino terminus of the TAF 1 protein binds to TBP and thereby inhibits TBP function in vitro , the physiological role of this domain remains obscure . ^^^ In our previous study , we screened for mutations that cause lethality when co expressed with the TAF 1 gene lacking TAND ( taf 1 DeltaTAND ) and identified two DeltaTAND synthetic lethal ( nsl ) mutations as those in the SPT 15 gene encoding TBP . ^^^ Interestingly , a set of genes affected in the taf 1 DeltaTAND mutant is similarly affected in the taf 12 HFD mutants but not in the nsl mutants of TBP . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| In agreement with this , biochemical experiments indicate that a subpopulation of TAF7L is tightly associated with TBP in both pachytene and haploid cells and TAF7L interacts with the TFIID subunit TAF 1 . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| As is evident from structural and functional studies , TBP ' s concave DNA binding surface is inhibited by a number of potential mechanisms , including homodimerization and binding to the TAND domain of the TFIID subunit TAF 1 ( yTAF ( 2 ) 145 / 130 ) . ^^^ The phenotypes included a low steady state level of the mutant TBP , transcriptional derepression , dominant slow growth ( partial toxicity ) , and synthetic toxicity in combination with a deletion of the TAF 1 TAND domain . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| The TAF N terminal domain ( TAND ) of TAF 1 may play a significant role in these two principal TFIID functions by regulating the access of TBP to the TATA element . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| The largest subunit of TFIID , TAF 1 , possesses an intrinsic protein kinase activity and is important for cell G 1 progression and apoptosis . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| Bromodomain factor 1 ( Bdf 1 ) associates with Saccharomyces cerevisiae TFIID and corresponds to the C terminal half of higher eukaryotic TAF 1 . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| Here we report that histone H2B is phosphorylated at evolutionarily conserved Ser 33 ( H2B S 33 ) by the carboxyl terminal kinase domain ( CTK ) of the Drosophila TFIID subunit TAF 1 . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| Here we show that a cell line with a temperature sensitive defect in TBP associated factor 1 ( TAF 1 ) , a component of the TFIID general transcription complex , exhibits hallmarks of an ATR mediated DNA damage response . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| The TAF N terminal domain ( TAND ) of TAF 1 includes two subdomains , TAND 1 and TAND 2 , which bind to the concave and convex surfaces of TBP , respectively . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| Arabidopsis HAF 2 gene encoding TATA binding protein ( TBP ) associated factor TAF 1 , is required to integrate light signals to regulate gene expression and growth . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| In TFIID , TAF 7 binds to TAF 1 and inhibits its intrinsic acetyl transferase activity . ^^^ We now report that although TAF 7 remains bound to TAF 1 and associated with TFIID during the formation of the PIC , TAF 7 dissociates from the PIC upon transcription initiation . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| Of the 14 TBP associated factor ( TAF ) subunits that compose TFIID , TAF 1 is one of the largest and most functionally diverse . ^^^ Yeast TAF 1 can be divided into four regions including a putative histone acetyltransferase domain and TBP , TAF , and promoter binding domains . ^^^ Paradoxically , deletion of the region of TAF 1 that is important for promoter binding interfered with the expression of many genes that are normally TFIID independent / SAGA ( Spt Ada Gcn 5 acetyltransferase ) dominated , suggesting that this region normally prevents TAF 1 ( TFIID ) from interfering with the expression of SAGA regulated genes . . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| The central role of the putative HAT containing TAF 1 subunit of TFIID in gene expression raises the fundamental question as to what extent , if any , TAF 1 contributes to acetylation in vivo and to what extent it is redundant with other HATs . ^^^ TAF 1 function has been linked to Bdf 1 , which binds TFIID and acetylated histone H 4 tails , but no linkage between TAF 1 and the H 4 HAT Esa 1 has been established . ^^^ |
|
| Interacting proteins: P21675 and P20226 |
Pubmed |
SVM Score :0.0 |
| In addition , other cofactors , such as TAF 1 , BTAF 1 , and negative cofactor 2 ( NC 2 ) , can also modulate TBP or TFIID binding to the core promoter . ^^^ |
|