Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P21583 and P49771	Pubmed	SVM Score :1.202789
These results demonstrate that soluble flt3L interacts with membrane bound SCF in supporting the long term growth of bone marrow progenitor cells . 1.202789^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
In previous studies using stroma free short term assays , Flt 3 ligand ( FL ) has been shown to induce proliferation of HPC at proportions similar to or less than c kit ligand ( steel factor , SF ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Using a plasma clot clonal assay and a long term bone marrow culture ( LTBMC ) system , we studied the effects of the recently cloned human flt 3 ligand ( FL ) alone and in combination with granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) , or stem cell factor ( c kit ligand [ KL ] ) on human megakaryocytopoiesis . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
We have performed a comprehensive analysis of cell lines and tissues to compare and contrast the expression patterns of Flt 3 ligand ( FL ) , c Kit ligand ( KL ) , and macrophage colony stimulating factor as well as their receptors , Flt 3 , c Kit , and c Fms . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
In quiescent HPCs the receptors for early acting [ flt 3 ligand ( FL ) , c kit ligand ( KL ) , bFGF , interleukin 6 ( IL 6 ) ] and multilineage [ IL 3 , granulocyte macrophage CSF ( GM CSF ) ] HGFs are expressed at significant levels but with different patterns , eg , kit and flt 3 are detected on a majority and minority of HPCs , respectively , whereas IL 3Rs and GM CSFRs are present on almost all HPCs . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
In this study , the effects of c kit ligand ( KL ) and flt 3 ligand ( FL ) were compared in the presence of IL 3 , GM CSF , and erythropoietin ( 3 / GM / EPO ) , using frequent medium exchange cultures of human bone marrow mononuclear cells ( BMMNC ) and CD 34 enriched cells . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
MGDF also enhanced interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , c kit ligand ( KL ) and FLT 3 ligand ( FL ) stimulated blast cell proliferation . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Tpo alone stimulated limited clonal growth , but synergized with c kit ligand ( KL ) , flt 3 ligand ( FL ) , or IL 3 to potently enhance clonogenic growth . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
This population was then used to evaluate the effects of supplemental cytokines ( interleukin 2 [ IL 2 ] , IL 3 , IL 7 , c kit ligand ) , FLT 3 ligand ( FL ) , and stroma derived factors on lymphoid differentiation in vitro . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
CD34+ cells purified from leukapheresis product were seeded at 1 10 10 ( 4 ) cells / mL in complete medium supplemented with GM CSF , TNF alpha , IL 4 , c kit ligand , and flt 3 ligand ( FL ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Progenitors were cultured in limiting dilutions with interleukin 7 ( IL 7 ) , flt 3 ligand ( FL ) , c kit ligand ( KL ) , IL 3 , IL 2 , and AFT 024 , a murine fetal liver line , which supports culture of transplantable murine stem cells . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
This features the use of a serum free medium supplemented with the cytokines flt 3 ligand , IL 3 , stem cell factor ( c kit ligand ) and IL 2 , together with IL 7 or oncostatin M ( OM ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
MPB purified CD34+ Thy 1+ cells were labeled with CFSE dye and cultured for 112 hours in serum deprived medium in the presence of the cytokine combinations of thrombopoietin ( TPO ) , flt 3 ligand ( FL ) , and c kit ligand ( KL ) , or TPO , FL , and interleukin 6 ( IL 6 ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Comparing several different combinations of cytokines added to 90 hour cultures of MPB CD 34 cells , thrombopoietin ( TPO ) , flt 3 ligand ( FL ) , and c kit ligand ( KL ) gave the best result , with the lowest percentage of apoptotic cells and a mean 1 . 2 fold increase in the number of CD34+ Thy 1+ cells . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Dendritic cells generated from CD34+ progenitor cells with flt 3 ligand , c kit ligand , GM CSF , IL 4 , and TNF alpha are functional antigen presenting cells resembling mature monocyte derived dendritic cells . ^^^ The combination of cytokines flt 3 ligand + c kit ligand + granulocyte macrophage colony stimulating factor ( GM CSF ) + interleukin 4 ( IL 4 ) + tumor necrosis factor alpha ( TNF alpha ) was used to maximize the output of mature DCs in the culture of CD34+ cells while minimizing the production of monocytes . ^^^ Differentiation was marked by a time dependent increase in allostimulatory function , which , at its peak , was more potent than in cultures of DCs generated from monocytes with GM CSF + IL 4 , but was comparable on a cell to cell basis to that of mature monocytes cultured in flt 3 ligand + c kit ligand + GM CSF + IL 4 + TNF alpha . ^^^ Altogether , these results suggest that DCs generated from CD34+ cells of patients with breast cancer with flt 3 ligand , c kit ligand , GM CSF , IL 4 , and TNF alpha are competent antigen presenting cells , particularly for CD8+ cytotoxic T lymphocytes , and resemble mature monocyte derived DCs in the assays described here . . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Also in CD34+ precursors , cultured in the presence of flt 3 ligand , c Kit ligand , GM CSF , IL 4 and TNF alpha , addition of anti CD 33 mAb prevented the recovery of mature dendritic cells . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Bone marrow and umbilical cord blood CD34+ cells were cultured in serum free medium under survival conditions with thrombopoietin ( Tpo ) alone , or under proliferating conditions with Tpo , c Kit ligand ( KL ) , and Flt 3 ligand ( FL ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Through such studies , c kit ligand ( KL ) , flt 3 ligand ( FL ) , thrombopoietin , and IL 11 have emerged as likely positive regulators of HSC self renewal . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
CD34+ hematopoietic cells were cultured in serum free medium with megakaryocyte growth and development factor ( MGDF ) alone for supporting the survival of primitive progenitors or with MGDF , c kit ligand ( KL ) and flt 3 ligand ( FL ) for inducing proliferation of primitive progenitors . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Among various combinations , the combination of c kit ligand , flt 3 ligand , c mpl ligand ( TPO ) , and interleukin ( IL ) 4 most potently generated the number of CD1a+CD14 DCs in cultures containing granulocyte macrophage colony stimulating factor ( GM CSF ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^ These findings demonstrated that the combination of c kit ligand plus flt 3 ligand plus TPO with GM CSF plus TNF alpha , followed by IL 4 , is useful for ex vivo generation of human DCs from mobilized CD34+ peripheral blood progenitors . . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Ex vivo expansion of stem and progenitor cells in co culture of mobilized peripheral blood CD34+ cells on human endothelium transfected with adenovectors expressing thrombopoietin , c kit ligand , and Flt 3 ligand . ^^^ Immortalized human bone marrow endothelial cells ( BMEC ) transfected with an expression vector carrying cDNA encoding the human telomerase reverse transcriptase ( hTERT ) and human umbilical vein endothelial cells ( HUVEC ) were transfected with combinations of adenovectors expressing murine c kit ligand ( KL ) , human thrombopoietin ( TPO ) , human Flt 3 ligand ( FL ) , and human granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Cell surface immunophenotyping of spleen derived fibroblasts revealed a low and consistent expression of IL 15 , Flt 3 ligand , and c kit ligand . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
METHODS : Human mobilized peripheral blood ( MPB ) CD 34 ( + ) cells were cultured with the cytokines thrombopoietin mimetic peptide ( mTPO ) , flt 3 ligand ( FL ) , and c kit ligand ( KL ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
This study investigated early ex vivo expansion kinetics of UCB AC133+ cells ( 2 4 10 10 ( 4 ) / ml ) , mononuclear cells ( MNC , 1 2 10 10 ( 6 ) / ml ) and AC133negative cells ( AC 133 ( neg ) , 2 4 10 10 ( 4 ) / ml ) in stroma free 8 d liquid culture ( fetal bovine serum supplemented Iscove ' s modified Dulbecco ' s medium ( IMDM ) with either ' K36EG ' [ c Kit ligand , interleukin 3 ( IL 3 ) , IL 6 , erythropoietin , granulocyte colony stimulating factor ] or ' TPOFL ' ( thrombopoietin , Flt 3 ligand ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
INTRODUCTION : We compared the effects of the early acting growth factors ( GF ) , Flt 3 ligand ( FL ) , c Kit ligand ( KL ) , and leukemia inhibitory factor ( LIF ) , and the late acting GF , granulocyte colony stimulating factor ( G CSF ) and megakaryocyte growth and development factor ( MGDF ) , added alone in human long term marrow culture ( LTMC ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
METHODS : AC 133 ( + ) stem / progenitor cells from six full term pregnancies UCB samples were immunomagnetically selected , followed by ex vivo expansion culture in the presence of thrombopoietin ( TPO ) , c kit ligand ( KL ) , flt 3 ligand ( FL ) and IL 6 . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Furthermore , over 8 weeks in liquid culture stimulated by a cytokine cocktail optimized for HSPC expansion , TPOFLK ( thrombopoietin 10 ng / ml , Flt 3 ligand 50 ng / ml , c Kit ligand 20 ng / ml ) Lin cells underwent slow proliferation but maintained / expanded more primitive HSPC than CD133+ cells . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Retroviral transduction of CD 34 ( + ) hemopoietic progenitor cells , during pre expansion by thrombopoietin , c kit ligand , and FLT 3 ligand , on recombinant fibronectin , but in the absence of FCS , resulted in gene expression by 20 30 % of the LCs . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
After 7 days of high density culture in microflasks , ( 10 ( 5 ) cells / ml , IMDM , FCS 10 % , thrombopoietin 10 ng / ml , flt 3 ligand 50 ng / ml , c kit ligand 20 ng / ml ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
To explore whether HDAC inhibitors could have a beneficial role in human HSC transplantation , mobilized peripheral blood CD34+ cells were cultured with thrombopoietin mimetic peptide , flt 3 ligand , and c Kit ligand , without or with various HDAC inhibitors . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
The importance of early hematopoietic growth factors , such as c kit ligand and flt 3 ligand , and their synergy with IL 15 in the development of human NK cells in the bone marrow has permitted the investigation of novel cytokine combinations for optimizing in vivo expansion of NK cell in the clinic . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Among this group are Steel factor ( also known as mast cell growth factor , stem cell factor and kit ligand ) , and the more recently described flt 3 ligand . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
All cases were also tested for their proliferative response to human FLT 3 ligand ( FL ) and KIT ligand ( KL ) and for colony formation in the presence of single or associated cytokines . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Similar enhancement was seen when combinations of cytokines , ie , G CSF plus kit ligand or G CSF plus Flt 3 ligand were used with anti VLA 4 in primates and mice . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Cell numbers in the suspension cultures initiated with Flt3high cells were maintained in the presence of Flt 3 ligand ( FL ) alone , and increased in response to FL plus kit ligand ( KL ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Compared with interleukin 3 ( IL 3 ) , IL 6 , and leukemia inhibitory factor ( LIF ) , the combination of thrombopoietin ( TPO ) , Flt 3 ligand ( FL ) , and Kit ligand ( KL ) effected a 73 fold increase in NGFR expression among CD34+ cells ( to 14 % ) and a 14 fold increase in NGFR expression among total cells ( to 10 % ) after LTC . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Medium for the ex vivo cultures contained either serum and six recombinant human hematopoietic growth factors ( GFs ) , including Flt 3 ligand ( FL ) , Kit ligand ( KL = stem cell factor ) , thrombopoietin ( Tpo ) , interleukin 3 ( IL 3 ) , granulocyte colony stimulating factor ( G CSF ) , and interleukin 6 ( IL 6 ) , or a serum free medium containing only FL , KL , and Tpo . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
In addition , TPO directly and potently synergizes with other early acting cytokines ( kit ligand , flt 3 ligand and interleukin 3 ) to promote multilineage growth of the same progenitor cell population . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Stimulation of mouse bone marrow cells with kit ligand , FLT 3 ligand , and thrombopoietin leads to efficient retrovirus mediated gene transfer to stem cells , whereas interleukin 3 and interleukin 11 reduce transduction of short and long term repopulating cells . ^^^ Large quantitative differences in reconstitution were observed after transplantation with graded numbers ( 1000 30 , 000 ) of EGFP ( + ) cells preconditioned with various combinations of Kit ligand ( KL ) , FLT 3 ligand ( FL ) , thrombopoietin ( TPO ) , interleukin 3 ( IL 3 ) , and IL 11 . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Human CD34+ cells from CB or PBSC were cultured in serum free medium , with or without hematopoietic growth factors ( FKT : FLT 3 ligand [ FL ] , KIT ligand [ KL ] , and thrombopoietin [ TPO ] ) , and with or without soluble Fas ligand ( sFasL ) or agonistic anti Fas antibody . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Expansion of haemopoietic stem cells from placental blood has been obtained with a combination of flt 3 ligand ( FL ) , thrombopoietin ( TPO ) , kit ligand ( KL ) with or without interleukin 6 ( IL 6 ) in serum replete medium . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Strain dependent variation existed among C57BL / 6 ( B 6 ) , DBA / 2 , and BXD recombinant inbred mice in the responsiveness of primitive progenitor cells to the early acting cytokines kit ligand , flt 3 ligand , and thrombopoietin . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
METHODS : CD34+ cell preparations purified from CB or PBSC were cultured in serum free medium containing FKT : FLT 3 ligand ( FL ) , KIT ligand ( KL ) , and thrombopoietin ( TPO ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Sorted NBM CD 34 ( + ) CD 133 ( + ) and CD 34 ( + ) CD 133 ( ) subsets were evaluated for Mk clonogenic capacity before and after in vitro proliferation in serum free liquid culture containing kit ligand , Flt 3 ligand , thrombopoietin , interleukin 3 , and interleukin 6 . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Phosphatidylinositol 3 kinase is essential for kit ligand mediated survival , whereas interleukin 3 and flt 3 ligand induce expression of antiapoptotic Bcl 2 family genes . ^^^ Cytokines such as interleukin 3 ( IL 3 ) , kit ligand ( KL ) , and flt 3 ligand ( FL ) promote survival of hematopoietic stem cells and myeloid progenitor cells . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
CD34+CD38 and CD34+CD38+ cells from umbilical cord blood were analyzed before and after culture with thrombopoietin ( TPO ) , FLT 3 ligand ( FL ) and kit ligand ( KL ; or stem cell factor ) in different combinations : TPO + FL + KL , TPO + FL and TPO , at concentrations of 50 ng / mL each . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
We studied the effect of two different cytokine combinations kit ligand ( KL ) , interleukin 3 ( IL 3 ) , and Flt 3 ligand supplemented with thrombopoietin and IL 11 ( combination 1 ) or granulocyte / macrophage colony stimulating factor ( GM CSF ) and G CSF ( combination 2 ) for their ability to affect speed and extent of engraftment using limited numbers ( 5 10 10 ( 4 ) ) of murine bone marrow ( BM ) light density ( LD ) cells or their progeny expanded ex vivo in the presence one or the other cytokine combination for 6 days . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Specifically , TNF alpha is a potent inhibitor of the clonal growth of primitive and committed murine and human bone marrow progenitors in combination with multiple cytokines , including granulocyte colony stimulating factor ( G CSF ) , CSF 1 , erythropoietin ( Epo ) , stem cell factor ( SCF ) , and flt 3 ligand ( FL ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Incubation with single recombinant cytokines from a broad spectrum of growth factors established that the strongest proliferation response of MUTZ 2 cells was elicited by FLT 3 ligand , granulocyte colony stimulating factor ( G CSF ) , macrophage CSF ( M CSF ) , interferon gamma ( IFN gamma ) and stem cell factor ( SCF ) , whereas granulocyte macrophage CSF ( GM CSF ) , M CSF , interleukin 3 ( IL 3 ) and SCF were the most effective growth factors in inducing proliferation of MUTZ 3 . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
In the present study , the flt 3 ligand ( FL ) , which , in combination with other cytokines , has overlapping activities with SCF on myeloid cell production from uncommitted progenitors , was investigated for its ability to induce selective stroma independent B cell commitment from uncommitted Lin Sca 1+ bone marrow progenitor cells . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Finally , the effect of IL 3 , IL 6 , SCF , and Flt 3 ligand ( FL ) on cycle induction was examined . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
LN provirus was detected by polymerase chain reaction in the marrow recovered from 9 of 10 mice transplanted with human CD34+ cells transduced with stromal support , 5 of 11 mice that received human cells transduced in suspension culture with FL , but none of the 10 mice that received human cells transduced in suspension culture without FL We conclude that FLT 3 ligand , in conjunction with IL 3 , IL 6 , and SCF , preserves the generative capacity of primitive human hematopoietic cells during in vitro transductions in suspension culture . . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Coexpression of flt 3 ligand / flt 3 and SCF / c kit signal transduction system in bile duct ligated SI and W mice . ^^^ It has been reported that the flt 3 ligand ( FL ) / flt 3 system can synergize with the SCF / c kit system and stimulate the proliferation of hematopoietic cells . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
We studied the effects of stem cell factor ( SCF ) and flt 3 ligand ( FL ) on the ex vivo expansion of human umbilical cord blood ( CB ) derived CD34+ cells in combination with various cytokines , including interleukin ( IL ) 3 , IL 6 , IL 11 , and c Mpl ligand ( thrombopoietin , TPO ) , in a short term serum free liquid suspension culture system . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Flt 3 ligand ( FL ) shares many features with stem cell factor ( SCF ) , a widely documented cofactor for peripheral blood progenitor cell ( PBPC ) mobilization . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
In methylcellulose cultures , flt3L stimulated formation of hematopoietic colonies only weakly , whereas it had an additive effect when combined with erythropoietin ( Epo ) , stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , interleukin 11 ( IL 11 ) , or granulocyte colony stimulating factor ( G CSF ) . ^^^ Flt3L was less effective than SCF and did not further enhance the number of hematopoietic colonies formed in response to SCF containing combinations of multiple cytokines . ^^^ In long term liquid suspension cultures , flt3L was less mitogenic than SCF but its effect on the maintenance of progenitors was superior that of SCF and of IL 3 , IL 11 , and G CSF . ^^^ The in vitro effects of flt3L on AA cells differ in many aspects from those of the structurally related cytokine SCF , suggesting a benefit in use of a combination of these two early acting growth factors . . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
In the first step , cells received a primary stimulation consisting of either stem cell factor ( SCF ) , Flt 3 ligand ( FL ) , interleukin 3 ( IL 3 ) , or IL 6 for 7 days . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
We compared two combinations of cytokines : interleukin 1alpha ( IL1alpha ) and interleukin 3 ( IL 3 ) with either Flt 3 ligand ( FLT3L ) or stem cell factor ( SCF ) . ^^^ Moreover , after 5FU pretreatment , the mean ( + / SEM ) percentage of Ph negativity was significantly greater for IL1alpha IL 3 FLT3L compared to IL1alpha IL 3 SCF ( 19 . 1 + / 2 . 5 % 5 14 . 8 + / 2 . 3 % , P=0 . 009 , n = 7 ) . ^^^ Thus , a subset of Ph negative cells was selected from CML PB by 5FU and expanded using the combination of cytokines IL1alpha IL 3 FLT3L and IL1alpha IL 3 SCF . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
The ligand for flt 3 ( FLT3L ) exhibits striking structural homology with stem cell factor ( SCF ) and monocyte colony stimulating factor ( M CSF ) and also acts in synergy with a range of other hematopoietic growth factors ( HGF ) . ^^^ To investigate the basis for the capacity of FLT3L to augment the de novo generation of myeloid progenitors from CD34+CD38 cells , single bone marrow CD34+CD38 cells were sorted into Terasaki wells containing serum free medium supplemented with interleukin 3 ( IL 3 ) , IL 6 , granulocyte colony stimulating factor ( G CSF ) , SCF ( 4 HGF ) + / FLT3L . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Low proliferative capacity was observed after 1 week in culture in the presence of stem cell factor ( SCF ) + Flt 3 ligand ( FL ) + G CSF . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Culture of lineage marker ( Lin ) , c kit+ , Sca2+ , interleukin ( IL ) 2 / 15Rbeta ( CD 122 ) marrow cells in IL 6 , IL 7 , stem cell factor ( SCF ) , and flt 3 ligand ( flt 3 L ) for 5 6 d followed by IL 15 alone for an additional 4 5 d expanded the starting population 30 40 fold and gave rise to a virtually pure population of NK1 . 1+ , CD 3 cells . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
To address this issue , we stimulated purified candidate HSC from human adult bone marrow with stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , and Flt 3 ligand ( FL ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Cells with a dendritic morphology and expressing HLA DR , CD1a , S 100 and CD 83 were maximally expanded under serum free conditions with the addition of SCF , GM CSF , TNF alpha , TGF beta and Flt 3 ligand ( fold increase of CD1a+ cells = 102 + / 32 after 2 weeks of culture ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Addition of FLT 3 ligand to IL 2 , IL 7 SCF , and stromal factors are important in early stages of NK development . . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
We analyzed the effect of tumor necrosis factor ( TNF ) alpha on the differentiation and viability of dendritic cells ( DC ) generated from cord blood CD34+ progenitors cultured for five days with GM CSF , Flt 3 ligand ( FL ) , and stem cell factor ( SCF ) , and then with GM CSF only [ TNF ( ) cultures ] . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
RT PCR analysis of cytokine and growth factor mRNA in MSCs and MDSCs revealed a very similar pattern of mRNAs including IL 6 , 7 , 8 , 11 , 12 , 14 , and 15 , M CSF , Flt 3 ligand , and SCF . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
We compared the effect of human flt 3 ligand ( FL ) and stem cell factor ( SCF ) on cord blood ( CB ) derived CD34+ cells expressing different levels of flt 3 or c kit tyrosine kinase ( TK ) receptor in clonal cell culture . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
We next compared the activation kinetics of individually sorted G0CD34+ cells stimulated with stem cell factor ( SCF ) , flt 3 ligand ( FL ) , or interleukin 3 ( IL 3 ) as single factors . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Supernatants of IL 1alpha stimulated EC potentiated the effects of an association of IL 1 , IL 3 , IL 6 , LIF , SCF , Flt 3 ligand , TPO , G CSF , GM CSF , M CSF and IL 11 on the proliferation of hematopoietic progenitors suggesting that EC may produce other soluble growth factors potentiating the action of the above set of cytokines . . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
The number of primitive human hematopoietic long term culture initiating cell derived colonies ( LTC IC derived colonies ) generated from CD34+ and CD34+ / HLA DRlo peripheral blood progenitor `` stem ' ' cells ( PBSCs ) increased during 7 days of treatment in this vector containing medium in the presence of IL 3 , SCF , and flt 3 ligand . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Flt 3 ligand , MGDF , Epo and G CSF enhance ex vivo expansion of hematopoietic cell compartments in the presence of SCF , IL 3 and IL 6 . ^^^ The aim of the study is to define the ability of Flt 3 ligand , MGDF , Epo and G CSF to modulate the expansion of different hematopoietic compartments in association with a basic cocktail of SCF + IL 3 + IL 6 ( S 36 ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Of the two hematopoietic growth factors , acting at the early stages of hematopoiesis , circulating levels of flt 3 ligand are highly elevated in patients with aplastic anemia , whereas those of stem cell factor ( SCF ) are essentially normal . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Circulating CD34+ cell populations characterized by a low rate ( up to five ) or high rate ( more than five ) of cell divisions were isolated from 8 d cultures in the presence of stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , granulocyte colony stimulating factor ( G CSF ) , erythropoietin ( EPO ) , Flt 3 ligand and Peg rHu megakaryocyte growth and development factor ( Peg rHuMGDF ) using the fluorescent dye 5 , 6 carboxyfluorescein diacetate succinimidyl ester ( CFDA SE ) and flow cytometric cell sorting . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Freshly isolated G0CD34 ( + ) cells were activated by interleukin 3 ( IL 3 ) , stem cell factor ( SCF ) , and flt 3 ligand ( FL ) for a 36 hour incubation period during which a fraction of cells progressed from G 0 into G 1 but did not complete a cell cycle . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
When combinations of cytokines were used over 7 days , between 5 % ( FLT 3 ligand [ FLT 3L ] , stem cell factor [ SCF ] , IL 3 , IL 6 , granulocyte colony stimulating factor [ G CSF ] , beta nerve growth factor [ betaNGF ] ) and 22 % ( FLT 3L + HGF ) of the cells remained undivided , between 15 % ( HGF , IL 1 , IL 11 , G CSF ) and 68 % ( SCF + TPO ) of the cells had doubled and between 27 % ( FLT 3L + TPO ) and 70 % ( HGF , IL 1 , IL 11 , G CSF ) died . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
The culture system features the use of a serum free medium supplemented with a cocktail of cytokines including flt 3 ligand , interleukin 3 ( IL 3 ) , stem cell factor ( SCF ) , and IL 2 . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NB from four infants was expanded by culturing the blood samples in the presence of thrombopoietin ( Tpo ) , interleukin 1beta ( IL1beta ) , IL 3 , IL 6 , flt 3 ligand and stem cell factor ( SCF ) for 7 d . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Purified human CD34+ cells were cocultured with PMVECs for 7 days in the presence of optimal concentrations of granulocyte / macrophage colony stimulating factor ( GM CSF ) + interleukin ( IL ) 3 + IL 6 + stem cell factor ( SCF ) + Flt 3 ligand . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
We compared different combinations of cytokines containing interleukin 1 alpha ( IL 1 ) , interleukin 3 ( IL 3 ) , stem cell factor ( SCF ) , leukemia inhibitor factor ( LIF ) , Flt 3 ligand ( FLT3L ) , and thrombopoietin ( TPO ) . 5FU decreased cell proliferation in the liquid culture but concurrently increased the expansion of CFU GM . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
CD34+Thy1+ , CD34+Thy1 , CD34+38 , CD34+38+ , CD34+DR+ , CD34+DR , CD34+Rhodamine123 ( Rh 123 ) and CD34+Rh123+ cells were incubated for 7 days in IMDM + 10 % FCS + IL 3 + IL 6 + G CSF + SCF ( 36GS ) + flt3L . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
In this study we have investigated the effect of the early acting haemopoietic cytokines flt 3 ligand ( FL ) , IL 3 and GM CSF on the SCF dependent differentiation of mast cells from cord blood mononuclear cells . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Different culture conditions were generated by adding G CSF , granulocyte macrophage colony stimulating factor ( GM CSF ) , Flt 3 ligand ( Flt 3 ) , megakaryocyte growth and development factor ( Peg rHuMGDF ) , GM CSF / erythropoietin ( EPO ) hybrid protein ( MEN 11303 ) , and interleukin 15 ( IL 15 ) to the basic stem cell factor ( SCF ) + interleukin 3 ( IL 3 ) + EPO combination . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
The relative effects of the cytokines M CSF , SCF , and Flt3L on the proliferation and development of the late myeloid progenitors granulocyte macrophage colony forming cells ( GM CFC ) were investigated . ^^^ Flt 3 ligand had a relatively small proliferative effect on enriched GM CFC compared to SCF and M CSF and had no ability to either stimulate colony formation or synergize with these two cytokines in promoting DNA synthesis , colony formation , or expansion in liquid culture . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Other cytokines that could be detected were IL 6 , M CSF , SCF , flt 3 ligand . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
To characterize the fate of early hematopoietic progenitor cells during ex vivo expansion in suspension culture , human CD 34 ( + ) enriched BPC were cultured in serum free medium in the presence of FLT 3 ligand ( FL ) , stem cell factor ( SCF ) and interleukin 3 ( IL 3 ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Target cells were exposed daily to vector containing supernatants and a combination of interleukin 3 ( IL 3 ) , interleukin 6 ( IL 6 ) , stem cell factor ( SCF ) and Flt 3 ligand ( FL ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
We examined the effect of recombinant human interleukin ( IL ) 11 alone or in combination with various colony stimulating factors ( CSFs ) , including IL 3 , granulocyte / macrophage ( GM ) CSF , granulocyte ( G ) CSF , stem cell factor ( SCF ) , flt 3 ligand ( FL ) , and thrombopoietin ( TPO ) , on colony formation by leukemic progenitor cells ( L CFU ) obtained from 33 patients with acute myelogenous leukemia ( AML ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Whilst samples without a mutation of the Flt 3 gene showed an increased cell production in response to either IL 3 and G CSF or IL 6 , SCF , TPO and Flt3L in long term stroma supported cultures , mutant samples failed to do so . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Therefore , we have attempted to maintain human cord blood or bone marrow CD 34 ( + ) cells ex vivo in serum free cultures containing stem cell factor ( SCF ) and FLT 3 ligand ( FL ) alone or together with a new recombinant molecule of soluble IL 6 receptor fused to IL 6 ( IL6RIL6 chimera ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
In short term cultures containing erythropoietin ( Epo ) , interleukin ( IL ) 1 , IL 3 , and IL 6 , or granulocyte ( G ) and granulocyte macrophage colony stimulating factor ( GM CSF ) together with stem cell factor ( SCF ) or Flt 3 ligand ( FL ) , expansion of CFUs was six to eightfold at week 1 . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
The production of haemopoiesis inhibitor by patient cells and the influence of high concentrations of stem cell factor ( SCF ) and Flt 3 ligand ( FL ) on cell survival and ex vivo expansion potential were also studied . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
To delineate factors involved in NK cell development , we established an in vitro system in which lineage marker ( Lin ) , c kit+ , Sca2+ bone marrow cells differentiate into lytic NK1 . 1+ but Ly 49 cells upon culture in IL 7 , stem cell factor ( SCF ) , and flt 3 ligand ( flt3L ) , followed by IL 15 alone . ^^^ A comparison of the ability of IL 7 , SCF , and flt3L to generate IL 15 responsive precursors suggested that NK progenitors express the receptor for flt3L . ^^^ To test the hypothesis that additional marrow derived signals are necessary for Ly 49 expression on developing NK cells , flt3+ progenitors were grown in IL 7 , SCF , and flt3L followed by culture with IL 15 and a marrow derived stromal cell line . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
In the presence of early acting cytokines interleukin 1beta ( IL 1beta ) , IL 3 , IL 6 , stem cell factor ( SCF ) , flt 3 ligand with and without thrombopoietin ( Tpo ) , we compared the expansion capacity of CD34+ enriched cells from CB , NB and bone marrow ( BM ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NALM 29 cells responded significantly to the proliferative stimuli of FLT 3 ligand and IL 7 , but not to GM CSF , IL 3 , IL 6 , PIXY 321 or SCF . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Monocytic differentiation of CD 34 ( + ) cells supported by FLT 3 ligand and interleukin 3 ( IL 3 ) was correlated to high levels of hypophosphorylated pRb , whereas neutrophilic differentiation , supported by granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) , was correlated to low levels . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
After single cell sorting , the CD 34 ( + ) / CD38 ( ) cells derived from FLV were exposed to flt 3 ligand , thrombopoietin , stem cell factor ( SCF ) , or medium containing a cytokine cocktail ( with SCF , interleukin 3 , interleukin 6 , granulocyte macrophage colony stimulating factor , and erythropoietin ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
CD34+ cells isolated from human CB were cultured in a stroma free liquid culture system with thrombopoietin ( TPO ) , flt 3 ligand ( FL ) , stem cell factor ( SCF ) , and / or granulocyte colony stimulating factor ( G CSF ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
The reduction of in vitro radiation induced Fas related apoptosis in CD34+ progenitor cells by SCF , FLT 3 ligand , TPO , and IL 3 in combination resulted in CD34+ cell proliferation and differentiation . ^^^ The early effects of exogenous cytokines at the hematopoietic stem and progenitor cell ( HSPC ) level following irradiation are still largely unknown , especially those of survival factors such as stem cell factor ( SCF ) and Flt 3 ligand ( FL ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Based on our earlier study of the conditions required for ex vivo expansion of Lin ( ) CD 34 ( + ) CD 38 ( ) cells and SRC , CD 34 ( + ) enriched lineage depleted umbilical cord blood cells were cultured for 2 to 6 days on fibronectin fragment in MGIN ( MSCV EGFP Neo ) retroviral supernatant ( containing 1 . 5 % fetal bovine serum ) and IL 6 , SCF , Flt 3 ligand , and G CSF . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
We describe here that lineage phenotype negative ( Lin ) ( ) c kit ( + ) hematopoietic progenitor cells ( HPCs ) from day 13 postcoitus ( dpc ) murine fetal liver ( FL ) can generate dendritic cell ( DC ) precursors when cultured in vitro in the presence of PA 6 stromal cells plus granulocyte / macrophage colony stimulating factor ( GM CSF ) + stem cell factor ( SCF ) + Flt 3 ligand ( Flt3L ) for 12 to 14 days , and develop into mature DCs when stimulated with GM CSF plus mouse tumor necrosis factor alpha ( mTNFalpha ) for an additional 3 to 5 days . ^^^ Once FL derived HPCs were cultured without GM CSF , NK1 . 1 ( + ) cells developed in the presence of PA 6 cells + SCF + Flt3L . ^^^ These NK1 . 1 ( + ) cells could develop into DC precursors at an earlier stage of differentiation by reculturing with PA 6 cells + SCF + Flt3L + GM CSF , but they would be irreversibly committed to NK cell precursors without GM CSF after 3 days , suggesting that GM CSF plays a critical role in controlling the transition of DC and NK cell precursors from 13 dpc FL derived Lin ( ) c kit ( + ) HPCs . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Culture of bone marrow precursor cells with cytokines , including granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 4 ( IL 4 ) and the tyrosine kinase receptor binding proteins Flt 3 ligand ( Flt 3L ) and stem cell factor ( SCF ) , has previously been shown , in both mouse and human , to result in the generation of large numbers of dendritic cells . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
To investigate the clinically applicable conditions that support substantial expansion of both primitive and more mature hematopoietic cells of umbilical cord blood ( UCB ) for transplantation in adults , enriched CD34+ cells from 8 fresh UCB samples and 4 expanded UCB products were cultured in defined serum free medium ( QBSF 60 ) in the presence of a cytokine combination of SCF , Flt 3 ligand ( FL ) , thrombopoietin ( TPO ) , IL 3 for up to 2 weeks . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
A large number of cells with the morphological , phenotypical , and functional characteristics of DCs was generated from both c kit ( low ) and c kit ( low ) populations when cultured with a combination of cytokines ( GM CSF , tumor necrosis factor a [ TNF a ] , interleukin 7 [ IL 7 ] , IL 3 , stem cell factor [ SCF ] , and flt 3 ligand ) ; the cytokine combination studies revealed that SCF and IL 3 in addition to GM CSF and TNF a are essential for DCs to be generated from these primitive populations . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Immunoselected CD34+ cells from peripheral blood stems cells ( PBSCs ) were cultured in media with or without serum , supplemented by IL 3 , IL 6 , IL 11 , SCF , TPO , Flt 3 ligand , and MIP 1alpha . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
The addition of other growth factors such as SCF and Flt 3 ligand can dramatically enhance DC recovery . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Twelve cases of human AML were tested for ability to be differentiated toward a professional APC phenotype in short term culture with addition of GM CSF and the following recombinant proteins : TNFalpha , IL 4 , CD 40 ligand , Flt 3 ligand and SCF . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
In the presence of IL 3 , IL 6 , SCF , or leukemia inhibitory factor ( LIF ) and Flt 3 ligand , hMSC facilitated expansion and retroviral transduction of human peripheral blood mobilized CD34+ cells . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Comparisons of structure function relationships of Flt3L with the homologous hematopoietic cytokines macrophage colony stimulating factor ( MCSF ) and stem cell factor ( SCF ) suggest that they have a common receptor binding mode that is distinct from the paradigm derived from the complex of growth hormone with its receptor . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
By day 21 , the endothelial based system receiving the FLT3L , SCF , IL 6 , and GM CSF cytokine combination contained five fold greater numbers of CD34+ than the stroma cell free culture cell system . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
The number of clonogenic megakaryocyte progenitors ( colony forming units megakaryocyte ; CFU Mk ) with high ( > 20 cells / colony ) and low proliferative capacity ( 5 20 cells / colony ) and the number of megakaryocytic ( CD61+ ) cells were significantly increased by including interleukin 3 ( IL 3 ) or IL 3 + IL 6 + IL 11 + Flt 3 ligand to cultures containing megakaryocyte growth and development factor ( MGDF ) plus stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
In this paper the effects of flt 3 ligand ( FL ) , stem cell factor ( SCF ) , interleukin ( IL ) 3 , IL 6 , granulocyte colony stimulating factor ( G CSF ) and granulocyte monocyte colony stimulating factor ( GM CSF ) on long term ex vivo expansion and differentiation of cord blood hematopoietic cells were investigated . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
The cytokines FLT3L + SCF + IL 7 + IGF 1 were added at day 0 , IL 4 + IL 5 + IL 6 + IL 10 and soluble CD 40 ligand at day 14 , and Staph . aureus Cowan particles on day 21 . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
The expansion conditions were : 1 . 5 10 10 ( 3 ) cells / ml in serum free medium containing stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , IL 6 , granulocyte stimulating factor ( G CSF ) , erythropoietin ( Epo ) + / Flt 3 ligand ( Flt 3 L ) for 10 days . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Three early acting ( stem cell factor ( SCF ) , Flt 3 ligand ( Flt 3 ) and fetal antigen 1 ( FA 1 ) ) and three lineage specific growth factors ( EPO , G CSF and thrombopoietin ( Tpo ) ) were analyzed by ELISA in 16 patients with multiple myeloma ( MM ) and 16 patients with non Hodgkin ' s lymphoma ( NHL ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
In physical or biological characteristics , it exhibited a unique character different from IL 3 , IL 6 , GM CSF , SCF or FLT 3 ligand those are known to have hematopoietic stem cell proliferation stimulating activity . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Among the cytokine combinations tested , SCF + IL 3 + sIL 6R / IL 6 + flt 3 ligand ( FL ) most effectively expanded CFU GM and CFU Mix . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
In cultures with SCF , Flt 3 ligand ( FL ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and tumor necrosis factor ( TNF ) alpha ( standard condition ) , CD 7 ( + ) CD45RA ( + ) progenitors expanded less ( 37 vs 155 fold ) but yielded 2 fold higher CD1a ( + ) DC percentages than CD 7 ( ) CD45RA ( + ) or CD 7 ( + ) CD45RA ( ) progenitors . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
CD34+ cells harvested from umbilical cord blood were kept in suspension cultures and stimulated with combinations of the cytokines stem cell factor ( SCF ) , FLT 3 ligand , interleukin 3 ( IL 3 ) , IL 6 , and IL 7 prior to transduction . ^^^ Then , prestimulation with SCF and FLT 3 ligand was compared . ^^^ Interestingly , FLT 3 ligand seemed to preserve the population of CD34+CD38 cells compared to SCF ( 16 . 56 % + / 2 . 02 versus 9 . 39 % + / 2 . 35 , p = 0 . 03 ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
PMVEC coculture supplemented with GM CSF + IL 3 + IL 6 + SCF + Flt 3 ligand induced > 93 % of the CD 34 ( + ) CD 38 ( ) population to enter the G ( 1 ) or G ( 2 ) / S / M phase while increasing this population from 1 . 4 % on day 0 to 6 . 5 % of the total population by day 5 . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
The cytokines studied were stem cell factor ( SCF ) , interleukin ( IL ) 3 , megakaryocyte growth and development factor ( MGDF ) , granulocyte colony stimulating factor ( G CSF ) , Flt 3 ligand , IL 6 , IL 11 , and erythropoietin ( EPO ) . ^^^ The effect of Flt 3 ligand and SCF factor on CD34+ cell production was also less than additive , although the response to both cytokines was greater than single cytokines . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Using a 1 day cytokine mediated prestimulation , consisting of human interleukin ( IL ) 3 , IL 6 , stem cell factor ( SCF ) , Flt 3 ligand ( FL ) , and thrombopoietin ( TPO ) , followed by a 3 day transduction procedure , we were able to detect up to 51 % CD 34 ( + ) cells expressing MDR 1 . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Hemopoietic cells were grown in long term cultures in the presence of various combinations of early acting cytokines such as FLT 3 ligand ( FLT 3 L ) and stem cell factor ( SCF ) and the differentiating growth factors GM CSF and TNF alpha . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
IL 11 and the Flt 3 ligand possessed T lineage promotional effects similar to IL 6 and SCF respectively . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Using a combination of FLT 3 ligand ( FL ) , Stem Cell Factor ( SCF ) , Thrombopoietin ( TPO ) and Interleukin 6 ( IL 6 ) , we cultured cord blood ( CB ) CD34+ cells for up to 12 weeks and transplanted their progeny into sublethally irradiated NOD / SCID mice . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
STUDY DESIGN AND METHODS : Cord blood CD34+ cells were cultured in Iscove ' s modified Dulbecco medium supplemented with 10 percent fetal calf serum ( FCS ) and IL 6 , SCF , megakaryocyte growth and development factor , and Flt 3 ligand . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Interleukin 3 ( IL 3 ) , stem cell factor ( SCF ) , and flt 3 ligand ( FL ) are potential candidates for expansion strategies due to their early acting and lineage unspecific hematopoietic stimulation . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Initial in vitro studies with rhesus CD34+ cells indicated that after 4 days of stimulatory culture in stem cell factor ( SCF ) , megakaryocyte growth and development factor ( MDGF ) , and flt 3 ligand ( FLT ) , transfer of the cells to SCF alone on retronectin ( FN ) support resulted in decreased active cycling and a halt to proliferation , without a loss of viability or induction of apoptosis . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Whereas Flt 3 ligand ( FL ) , stem cell factor ( SCF ) , and thrombopoietin ( TPO ) are considered indispensable , the role of interleukin 3 ( IL 3 ) is still controversial : it has been reported either to support or abrogate the reconstituting ability of stem cells . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Human peripheral blood ( PB ) CD34+ cells ( > or = 90 % purity ) were cultured for up to 4 days in serum free culture medium supplemented with thrombopoietin ( TPO ) , stem cell factor ( SCF ) , Flt 3 ligand ( Flt 3L ) , with or without PIXY 321 ( IL 3 / GM CSF fusion protein ) and human serum . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Cell cycle activation of peripheral blood stem and progenitor cells expanded ex vivo with SCF , FLT 3 ligand , TPO , and IL 3 results in accelerated granulocyte recovery in a baboon model of autologous transplantation but G0 / G1 and S / G2 / M graft cell content does not correlate with transplantability . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
We investigated the maintenance of primitive SCID repopulating cells ( SRC ) and long term culture initiating cells ( LTCIC ) in PBSC expanded with early acting cytokines , thrombopoietin ( TPO ) , stem cell factor ( SCF ) and FlT 3 ligand ( FL ) with or without interleukin 3 ( IL 3 ) and IL 6 in short term ( 6 d ) stroma free serum free cultures . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Blast cells were expanded in serum free medium supplemented with Flt3L , G CSF , GM CSF , IL 3 , IL 6 and SCF for 7 days and subsequently stimulated with Flt3L , GM CSF and TGF beta for a further 14 days , with the addition of TNF alpha for the final 48 h of culture . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
STUDY DESIGN AND METHODS : Enriched CB CD34+ cells were cultured in four media ( Iscove ' s modified Dulbecco ' s medium with FCS , Gibco ; 10 Vivo 10 , BioWhittaker ; QBSF 60 , Quality Biological ; and StemSpan SFEM , Stem Cell Technologies ) with four cytokine combinations ( thrombopoietin [ TPO ] , SCF , Flt 3 ligand [ FL ] with and without G CSF , and / or IL 6 ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
The growth kinetics of two populations of muscle stem cells were characterized in culture medium supplemented with epidermal growth factor ( EGF ) , fibroblast growth factor 2 ( FGF 2 ) , insulin like growth factor 1 ( IGF 1 ) , FLT 3 ligand , hepatocyte growth factor , or stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
In the present study , several cytokines , flt 3 ligand , stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , IL 6 , IL 11 , leukemia inhibitory factor , G CSF , and erythropoietin , which are known to affect megakaryocytopoiesis , have been evaluated to elucidate their effects on the TPO induced apoptosis . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
The quantification analyses have revealed the increase of the amounts of IL 6 and flt 3 ligand in their serum , but not significant increase of the amount of SCF . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Stem Cell Factor ( SCF ) + IL 6 and FLT 3 ligand , which have no effect on colony formation by themselves , were used to enhance the effects of IL 3 and GM CSF , respectively . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
In contrast , rhSCGF alpha suppressed stem cell factor ( SCF ) stimulated erythroid bursts , indicating some competitive interaction between SCGF and SCF . rhSCGF alpha was synergistic with interleukin 3 and the flt 3 ligand to enhance GM colony growth , but not synergistic with those inducing ex vivo expansion of GM progenitor cells . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
CIP 29 mRNA expression is cytokine regulated in hematopoietic cells , being up regulated by Epo in UT7 / Epo cells , and by thrombopoietin ( Tpo ) , FLT 3 ligand ( FL ) and stem cell factor ( SCF ) in primary human CD 34 ( + ) cells . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
The LC cells were cultured in serum free culture medium supplemented with a combination of early acting cytokines including thrombopoietin ( TPO ) , flt 3 ligand ( FL ) , and stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Lin ( ) Sca 1 ( + ) HPC cultured 9 days in 100 ng / mL stem cell factor ( SCF ) , 20 ng / mL interleukin 3 ( IL 3 ) , 50 ng / mL monocyte colony stimulating factor ( M CSF ) , 5 ng / mL granulocyte monocyte colony stimulating factor ( GM CSF ) , and 25 ng / mL FLT 3 ligand ( FLT 3 L ) proliferate 387 fold and differentiate into DC precursors . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
MATERIAL AND METHODS : To perform our experiments we used a stroma free cell culture system in which the combination of FLT 3 ligand ( FL ) , stem cell factor ( SCF ) and thrombopoietin ( TPO ) induces massive expansion and proliferation of cord blood HP . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Proliferative response of blast clonogenic cells to various hematopoietic growth factors ( HGF ) , including stem cell factor ( SCF ) and flt 3 ligand ( FL ) was investigated in 100 patients with acute myeloid leukemia ( AML ) and chronic myelogenous leukemia ( CML ) in myeloid crisis ( MC ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Recipient mice were treated for 1 or 7 days with growth factors ( stem cell factor [ SCF ] plus interleukin 11 [ IL 11 ] , SCF plus Flt 3 ligand [ FL ] , or granulocyte colony stimulating factor [ G CSF ] ) prior to low dose TBI ( 4 Gy ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
METHOD : The CD ( 34 ) ( + ) CD ( 59 ) ( + ) and CD ( 34 ) ( + ) CD ( 59 ) ( ) cells from PNH patients and CD ( 34 ) ( + ) CD ( 59 ) ( + ) cells from normal volunteers were sorted as each single cell into a well of 96 well culture plates containing culture medium supplemented with SCF , IL 3 , Epo , GM CSF , G CSF , IL 6 , Tpo and Flt 3 ligand . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Cord blood CD34+ cells were incubated with supernatants containing retrovirus for 48 h with cytokines such as IL 3 , IL 6 , SCF , Flt 3 ligand ( FL ) , bFGF and IGF 1 . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
The first consisted of a 7 day culture in the presence of stem cell factor ( SCF ) , Flt 3 ligand , thrombopoietin ( TPO ) , interleukin 3 ( IL 3 ) , and IL 6 , which induced preferentially the expansion of immature hematopoietic cells [ 3 . 1 + / 1 . 4 , 10 . 0 + / 5 . 1 , 2 . 2 + / 1 . 9 , and 1 . 0 + / 0 . 3 fold expansion for mononuclear cells ( MNC ) , colony forming units granulocyte macrophage ( CFU GM ) , burst forming units erythroid ( BFU E ) , and long term culture initiating cells ( LTC IC ) respectively ] . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Apoptosis may be significantly reduced with a combination of 4 cytokines ( 4F ) : Stem Cell Factor ( SCF ) , FLT 3 Ligand ( FL ) , thrombopoietin ( TPO ) , and interleukin 3 ( IL 3 ) , each at 50 ng 10 mL ( 1 ) ( 15 % survival versus < 3 % untreated cells , 24 h post irradiation at 2 . 5 Gy ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Up regulation of survivin by thrombopoietin ( Tpo ) , Flt 3 ligand ( FL ) , and stem cell factor ( SCF ) occurred in underphosphorylated retinoblastoma protein ( Rb ) ( positive ) , Ki 67 ( negative ) , and cyclin D ( negative ) CD 34 ( + ) cells . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Consistent with the in vivo repopulating deficiency , when Sca 1 ( + ) c kit ( + ) lin ( ) ( KLS ) cells were isolated and stimulated with growth factors in vitro , up to a 13 fold reduced expansion of total nucleated cells was observed in response to cocktails containing interleukin 3 ( IL 3 ) , IL 6 , stem cell factor ( SCF ) , Flt 3 ligand , and thrombopoietin . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
CD34+ cells from cord blood ( CB ) , bone marrow ( BM ) , or peripheral blood ( PB ) were expanded in a medium containing stem cell factor ( SCF ) , flt 3 ligand ( Flt3L ) and thrombopoietin ( TPO ) with or without HESS 5 , a murine BM stromal cell line , for 2 weeks ( the first expansion step ) , then differentiated to DC in a medium containing granulocyte macrophage colony stimulating factor ( GM CSF ) , flt 3 ligand ( Flt3L ) , stem cell factor ( SCF ) , tumor necrosis factor alpha ( TNF alpha ) , IL 4 , and lipopolysaccharide ( LPS ) for 9 days ( the second differentiation step ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
In contrast , we previously reported that Survivin is expressed throughout the cell cycle in normal CD 34 ( + ) hematopoietic stem and progenitor cells stimulated by the combination of Thrombopoietin ( Tpo ) , Stem Cell Factor ( SCF ) and Flt 3 ligand ( FL ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
This work was aimed at evaluating the in vivo survival effect of combinations of early acting cytokines whose antiapoptotic activity has been demonstrated in vitro : stem cell factor ( SCF [ S ] ) , FMS like tyrosine kinase 3 ligand ( FLT 3 ligand [ F ] ) , thrombopoietin ( TPO [ T ] ) , interleukin 3 ( IL 3 [ 3 ] ) , and stromal derived factor 1 ( SDF 1 ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
OBJECTIVE : To analyze the expression of the CD ( 40 ) and its ligand ( CD ( 40 ) L ) on bone marrow stromal cells ( BMSC ) , and investigate interleukin 3 ( IL 3 ) , IL 6 , Flt 3 ligand ( FL ) and stem cell factor ( SCF ) production of BMSC after stimulated with CD ( 40 ) agonistic monoclonal antibody ( 5C11 ) and its role in the regulation of hematopoiesis . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
E2A expression in vitro was also assessed in cultures supplemented with IL 7 + / recombinant murine SCF ( rmSCF ) , insulin like growth factor 1 ( rhIGF 1 ) , or Flt 3 ligand ( rhFlt 3 L ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Cells were cultured with stem cell factor ( SCF ) , thrombopoietin ( TPO ) , Flt 3 ligand ( Flt 3 ) , and G CSF . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
The highest proliferation was usually observed in the presence of Flt 3 Ligand ( Flt 3 L ) when single cytokines were examined , and these responses could be further increased especially by combining Flt 3 L with interleukin 3 ( IL 3 ) , IL 7 or stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Cytokines assumed to have a stimulatory effect on HPC stem cell factor ( SCF ) , fetal liver tyrosine kinase 3 ligand ( flt 3 ligand ) , thrombopoietin ( TPO ) , stromal cell derived factor 1 ( SDF 1 ) , oncostatin M ( OSM ) , a suppressive effect on HPC proliferation macrophage inflammatory protein 1alpha ( MIP 1alpha ) , transforming growth factor beta 1 ( TGFbeta 1 ) , tumour necrosis factor alpha ( TNFalpha ) and to be involved in migration of HPC ( SCF , flt 3 ligand , MIP1alpha , SDF 1 ) were examined at the level of mRNA expression by means of real time RT PCR . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
METHODS : Adult normal bone marrow ( BM ) and mobilized peripheral blood ( MPB ) CD 34 ( + ) cells were cultured in a stroma free liquid culture in the presence of Flt 3 ligand ( FL ) , thrombopoietin ( TPO ) , stem cell factor ( SCF ) , interleukin 6 ( IL 6 ) , or interleukin 3 ( IL 3 ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
CD 34 progenitor cells were first amplified in vitro with stem cell factor ( SCF ) , Flt 3 ligand ( FL ) , thrombopoietin and IL 6 / soluble IL 6 receptor fusion protein , and cells were then induced to differentiate into DC with IL 4 and GM CSF . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
To study further the mechanisms underlying the unique patterns of hematopoietic regeneration after cyclophosphamide , mRNA levels for stem cell factor ( SCF ) , Flt 3 ligand , and macrophage inflammatory factor ( MIP ) 1 alpha cytokines were determined in bone marrow and spleen . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
IL 15 was essential and , in combination with either flt 3 ligand ( FL ) or stem cell factor ( SCF ) , was sufficient to induce the development of NKT cells . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
STUDY DESIGN AND METHODS : CD34+ cells were analyzed for the expression of CD 38 , CD 133 , and CD 117 and cultured in serum free culture medium with four cytokine combinations : SCF plus thrombopoietin plus flt 3 ligand ( STF ) , STF plus IL 3 , STF plus IL 6 , and STF plus IL 6 plus IL 3 . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
CD 34 ( + ) cells purified from ten CBs by immunomagnetic selection were cultured in serum free essential media in the presence of a combination of the several cytokines including EPO , thrombopoietin , flt 3 ligand ( FL ) , stem cell factor ( SCF ) , granulocyte colony stimulating factor , interleukin ( IL ) 3 and / or IL 11 . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
DESIGN AND METHODS : To find the best cytokine combination capable of generating large numbers of Mks , baseline CB CD34+ ( bCD34+ ) cells and CD34+ and CD 34 cells , immunoselected after 4 weeks of expansion with thrombopoietin ( TPO ) , stem cell factor ( SCF ) and Flt 3 ligand ( FL ) ( eCD34+ , eCD 34 ) , were further cultured in the presence of different cytokine combinations ( containing interleukin ( IL ) 3 , SCF , TPO and IL 6 ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
When vibrissal follicles of adult mice were cultured in the presence of stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , IL 7 , granulocyte macrophage colony stimulating factor , and Flt 3 ligand , CD45+ / lineage / c kit+ / FcepsilonRI+ cells became detectable on the outgrowing fibroblasts in 10 days and expanded progressively thereafter . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
METHODS : The cells , plated for 7 days in serum free medium with SCF , IL 3 , G CSF , Flt 3 ligand and thrombopoietin in various combinations were assessed for the expression of CD 34 , CD 38 and CD 13 . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
METHODS : Purified human peripheral blood CD34+ cells were cultured in the presence of recombinant human IL 3 , IL 5 , rhGM CSF , SCF , and FLT 3 ligand . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Our results showed that the use of high concentrations of stem cell factor ( SCF ) and Flt 3 ligand ( FL ) in early CB TPO supplemented cultures was more favorable to monocytic and granulocytic cell expansion . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
CD34+ cells were then cultured in serum free liquid medium containing various combinations of early acting cytokines , including thrombopoietin ( TPO ) , stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) and flt 3 ligand ( FL ) , with or without bFGF , for 6 and 12 d . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
METHODS : AC133+ cells selected from fresh cord blood ( CB ) samples were cultured in QBSF 60 serum free media in the presence of Flt 3 ligand + SCF + TPO ( FST ) , with initial addition of IL 3 for up to 2 week . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
CD34+ CD 71 CD 45 cells were cultured in defined serum free media supplemented with 300 ng each of Flt 3 ligand and stem cell factor ( SCF ) , 60 ng of interleukin ( IL ) 3 , and 20 ng each of IL 6 and G CSF . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
CD 34 ( + ) enriched cell populations from bone marrow were cultured in the presence and absence of human stroma in serum free media containing bFGF , SCF , LIF and Flt 3 ligand for several days . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
G CSF / SCF mobilized CD 34 ( + ) rhesus peripheral blood cells were cultured in the presence of SCF , Flt 3 ligand , and MGDF on Retronectin coated flasks . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
We cultured HPCs in a serum free medium in the presence of IL 3 and a combination cytokines including Flt3L , SCF , and TNF alpha with or without TGF beta 1 . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
To study the biological role of human cultured bone marrow mesenchymal stem cell ( BM MSC ) in hematopoiesis by investigation of its expression of multiple hematopoietic growth factors , RT PCR was used to analyze the expression of SCF , Flt 3 ligand , TPO , LIF , G CSF , GM CSF , IL 3 , IL 6 and IL 11 at mRNA level for human BM MSC from healthy donors and patients with leukemia and lymphoma . ^^^ The results clearly showed that the cultured BM MSC expressed mRNA of SCF , Flt 3 ligand , TPO , LIF , IL 6 and IL 11 at passages 3 up to 15 , but did not express G CSF , GM CSF and IL 3 . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
The cells were stimulated by SCF , G SCF , IL 3 and Flt 3 ligand ( FL ) added once at Day 0 . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
RESULTS : Proliferation by CFU GM and BFU E was enhanced synergistically when common beta chain receptor cytokines ( IL 3 or GM CSF ) were combined with G CSF or EPO , but not with gp 130 receptor cytokines ( LIF or IL 6 ) or tyrosine kinase receptor cytokines ( SCF , HGF , Flt 3 ligand , or PDGF ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
In serum free single cell suspension culture , VIP enhanced clonal growth of CD34+ CB cells in synergy with FLT 3 ligand ( FL ) , stem cell factor ( SCF ) , and thrombopoietin ( TPO ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
To identify putative predictors of response , patient plasma was analyzed for levels of secreted ligands of SU 5416 targets ; SCF and FLT 3 ligand . ^^^ Baseline levels of SCF in patients with stable or progressive disease were significantly higher than those in normal donors , whereas FLT 3 ligand levels in patients who exhibited progressive disease were significantly lower than those in normal donors . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Hematopoietic stem cells mitogens SCF and flt 3 ligand ( FL ) were also mitogenic for fetal hepatocytes , but did not further enhance the effect of IL 6c on cell proliferation . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
To explore the efficacy of the different combinations of stem cell factor ( SCF ) , FLT 3 ligand ( FL ) and interleukin ( IL ) 2 , 7 , 15 on the induction and expansion of cord blood ( CB ) derived CIK / NK cells in vitro , according to the different combinations of cytokines , combinations of cytokines were divided into 3 groups : group A ( SCF + IL 2 + IL 7 + IL 15 ) , group B ( SCF + FL + IL 2 + IL 7 + IL 15 ) and group C ( IL 2 + IL 7 + IL 15 as control group ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Following transduction , Transduced cells were divided equally into four groups as follow : ( 1 ) No drug group , ( 2 ) AP 2018 alone group , ( 3 ) SCF + Flt 3 Ligand group , ( 4 ) AP 20187 + SCF + Flt 3 Ligand group . ^^^ RESULTS : Only the group of AP 20187 combined SCF and Flt 3 Ligand have a significant sustained outgrowth . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Several cytokines , especially stem cell factor ( SCF ) and FLT 3 ligand ( FL ) , have been identified as essential to produce large numbers of myeloid precursors and even to increase DC yield obtained by the action of granulocyte macrophage colony stimulating factor ( GM CSF ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
We report the results of experiments with CD 34 ( + ) cells from cord blood ( CB , n = 13 ) , bone marrow ( BM , n = 4 ) , and mobilized peripheral blood stem cells ( PBSC , n = 5 ) using two combinations of cytokines : ( A ) granulocyte colony stimulating factor ( G CSF ) , interleukin 3 ( IL 3 ) , interleukin 6 ( IL 6 ) , stem cell factor ( SCF ) , erythropoietin ( EPO ) , insulin like growth factor 1 ( IGF 1 ) , basic fibroblast growth factor ( FGF b ) and ( B ) combination A plus FLT 3 ligand ( FL ) and megakaryocyte growth and development factor ( PEG rhMGDF ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
While hematopoietic development was diminished without serum , here we demonstrate with the stromal cell coculture model that addition of the growth factors stem cell factor ( SCF ) , thrombopoietin ( TPO ) , and Flt 3 ligand ( Flt3L ) to serum free media does allow isolation of hematopoietic progenitors . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
To overcome this limitation , we developed an ex vivo expansion system for HSC , in which CB CD34+ cells are cultured on feeder cells ( HESS 5 cells ) in the presence of cytokines ( TPO , SCF and Flt 3 ligand ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
In the first step , CB CD34+ cells were expanded by stimulation with early acting cytokines such as stem cell factor ( SCF ) , flt 3 ligand ( FL ) , and thrombopoietin ( TPO ) to amplify the hematopoietic progenitor cells . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Ex vivo culture with the cytokines TPO , FLT 3 ligand , and SCF ( T / F / S ) plus IL 3 or IL 7 substantially increased the yield of transduced bone marrow ( BM ) CD 34 ( + ) cells purified from ADA SCID patients or healthy donors , compared to T / F / S alone . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Because hESCs express growth factor receptors , including those for basic fibroblast growth factor ( bFGF ) , stem cell factor ( SCF ) , and fetal liver tyrosine kinase 3 ligand ( Flt3L ) , we evaluated these and other growth factors for their ability to maintain undifferentiated hESCs in the absence of conditioned medium . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Within the first 6 hours post lethal dose radiation , there is no significant change of gene expression of the known radioprotective factors TPO , SCF , IL 3 , Flt 3 ligand , and SDF 1 . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
To evaluate the effect of cytokines , CD34+ cells were cultured with various cytokine combinations , such as stem cell factor ( SCF ) , flt 3 ligand ( FL ) , and thrombopoietin ( TPO ) with vascular endothelial growth factor ( VEGF ) , interleukin 1 beta , fibroblast growth factor basic ( FGF b ) as basic cytokines . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
For 3 days , HPC were maintained in serum free medium supplemented with a single cytokine ( SCF ) or a cytokine combination ( SCF , Flt 3 ligand , IL 6 ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Here , we report that the treatment of hES cells during EBs development with a combination of low dose hematopoietic cytokines , including stem cell factor ( SCF ) , Flt 3 ligand , vascular endothelial growth factor ( VEGF ) and human bone marrow stromal cells ( hBMSCs ) , generated cell clusters that contained 8 . 81 % KDR positive hemangioblasts , 9 . 94 % CD 34 positive hematopoietic stem cells and 25 . 7 % CD 45 positive mature hematopoietic cells , and expressed hematopoietic genes such as KDR , stem cell leukemia ( scl ) and runt related transcription factor 1 ( Runx 1 ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Mesenchymal stem cells support expansion of in vitro irradiated CD 34 ( + ) cells in the presence of SCF , FLT 3 ligand , TPO and IL 3 : potential application to autologous cell therapy in accidentally irradiated victims . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Our results demonstrated that CTCE 0214 synergized with thrombopoietin ( TPO ) , stem cell factor ( SCF ) , or flt 3 ligand ( FL ) on the survival of stem and progenitor cells in culture . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
The CD 34 ( + ) cells were plated for 10 days in presence of thrombopoietin ( TPO ) alone and combined with stem cell factor ( SCF ) , Flt 3 ligand ( FL ) , interleukin 3 ( IL 3 ) , IL 6 , and IL 11 . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Briefly , CD133+ cells , purified from CB using Miltenyi Biotec ' s ( Bergisch Gladbach , Germany ) CliniMACS separation device and the anti CD 133 reagent , were cultured for 3 weeks in a clinical grade closed culture bag system , using the chelator based technology in combination with early acting cytokines ( SCF , thrombopoietin , IL 6 and FLT 3 ligand ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Afterwards , a statistical analysis of the two level factorial designs revealed that in the presence of TPO , MK maturation was significantly stimulated by stem cell factor ( SCF ) , interleukin ( IL ) 6 , and IL 9 , whereas Flt 3 ligand ( FL ) had a positive effect only on the expansion of MK progenitors . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Human UCB CD 34 ( + ) cells were cultured on MS 5 mouse stroma cells in the presence of stem cell factor ( SCF ) , flt 3 ligand ( FL ) , and thrombopoietin ( TPO ) for 4 to 16 weeks . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
OBJECTIVE : To evaluate canine histiocytic sarcoma cell lines and tumor samples for dysregulation of the Kit / stem cell factor ( SCF ) , Flt3 / Flt3 ligand ( Flt3L ) , and Met / hepatocyte growth factor ( HGF ) receptor tyrosine kinase signaling pathways , as these are known to contribute to the differentiation and survival of normal dendritic cells as well as malignant transformation of dendritic cells in mouse models . ^^^ PROCEDURE : Histiocytic sarcoma cell lines were evaluated for expression of Kit / SCF , Flt3 / Flt3L , and Met / HGF by use of reverse transcriptase PCR procedures . ^^^ CONCLUSIONS AND CLINICAL RELEVANCE : These data suggest that dysregulation of Kit / SCF , Flt3 / Flt3L , and Met / HGF signaling pathways is unlikely to occur in histiocytic sarcomas of dogs and that inhibitors of the Kit , Flt 3 , and Met pathways are unlikely to provide clinical benefit to dogs with histiocytic sarcomas . . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Lyn is renowned for its role in B cell antigen receptor and FcepsilonRI signaling ; however , it is becoming increasingly apparent that Lyn also functions in signal transduction from growth factor receptors including the receptors for GM CSF , IL 3 , IL 5 , SCF , erythropoietin , CSF 1 , G CSF , thrombopoietin and Flt 3 ligand . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
OBJECTIVES : The effect of stem cell factor ( SCF ) , flt 3 ligand ( FL ) , and interleukin ( IL ) 3 ( SF 3 ) in combination with hepatocyte growth factor ( HGF ) , thrombopoietin ( TPO ) , and Hyper IL 6 on maintenance and differentiation of early human peripheral blood derived progenitor cells was investigated . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
We have investigated the influence of different hematopoietic growth factors , including granulocyte colony stimulating factor ( G CSF ) , stem cell factor ( SCF ) , Flt 3 ligand ( Flt 3L ) and thrombopoietin ( TPO ) , on the course of relapsing experimental autoimmune encephalomyelitis , a mouse model of multiple sclerosis . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Stem cell factor , but not flt 3 ligand , induces differentiation and activation of human mast cells . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Differential effects of Flk 2 / Flt 3 ligand and stem cell factor on murine thymic progenitor cells . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
In an effort to obtain human B lymphoid , as well as T lymphoid and myeloid cell development in the mice , CD34+ cells were coinjected with human marrow stromal cells engineered to secrete human IL 2 , IL 7 , stem cell factor or FLT 3 ligand , + / IL 3 . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
A numerical expansion of stem cell subsets was observed in the presence of interleukin 3 ( IL 3 ) , stem cell factor , and IL 6 , while stroma contact , stromal soluble factors , or combined addition of FLT 3 ligand and thrombopoietin improved this parameter . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Distinct actions of Flt 3 ligand and stem cell factor on myeloid lineage selection and maturation of granulocytes versus macrophages . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Newer growth factors such as stem cell factor , thrombopoietin , Flt 3 ligand , and interleukins have shown promising results in the laboratory , and some have been used in clinical trials . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
However , ex vivo expansion of the sorted subsets with interleukin 3 , stem cell factor and FLT 3 ligand for 2 weeks resulted in a significant production of O 2 and H2O2 / HOCl by CD34+ / CD117low cell fraction , compared to the same sorted but not expanded counterparts . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Cord blood CD34+ cells were cultured with granulocyte macrophage colony stimulating factor ( GM CSF ) and tumor necrosis factor alpha ( TNF alpha ) , to which stem cell factor and Flt 3 ligand were added for 5 days . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Improved gene transfer into baboon marrow repopulating cells using recombinant human fibronectin fragment CH 296 in combination with interleukin 6 , stem cell factor , FLT 3 ligand , and megakaryocyte growth and development factor . ^^^ The highest level of gene transfer was obtained after 24 hours of prestimulation followed by 48 hours of incubation on CH 296 in vector containing medium in the presence of multiple hematopoietic growth factors ( interleukin 6 , stem cell factor , FLT 3 ligand , and megakaryocyte growth and development factor ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
However , the more restricted cellular distribution of the FLT 3 receptor may target the effects of FLT 3 ligand to primitive hematopoietic cells and to myeloid and lymphoid progenitor cells , in contrast to the pleiotropic effects of the c kit receptor ligand , stem cell factor . . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
By reverse transcription polymerase chain reaction , the expression by MMHs of cytokines involved in the survival and self renewal of early progenitor cells ( stem cell factor and FLT 3 ligand ) as well as those acting at different stages of progenitor differentiation ( interleukin [ IL ] 1beta , IL 3 , leukemia inhibitory factor , IL 6 , granulocyte macrophage colony stimulating factor , granulocyte colony stimulating factor , macrophage colony stimulating factor , and thrombopoietin ) was shown . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Other mouse models have indicated an unexpected role for flt 3 ligand in early NK cell development as well as minor roles for stem cell factor and IL 7 in expanding NK cell progenitor numbers . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
The results show that using a growth factor combination including granulocyte macrophage colony stimulating factor plus tumor necrosis factor alpha plus interleukin 4 plus stem cell factor plus Flt 3 ligand , more than 70 % of DCs derived from CB or MPB CD34+ cells can be transduced . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Long term culture of human CD 34 ( + ) progenitors with FLT 3 ligand , thrombopoietin , and stem cell factor induces extensive amplification of a CD 34 ( ) CD 14 ( ) and a CD 34 ( ) CD 14 ( + ) dendritic cell precursor . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
The assay is done in contact with murine AFT 024 fetal liver stromal cells and with Flt 3 Ligand , stem cell factor , and interleukin 7 . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
RESULTS : Under our optimized conditions of mouse BM incubation ( stem cell factor plus interleukin 11 , either with or without macrophage inflammatory protein 1alpha or Flt 3 ligand ) , both the short term and the mid term repopulating ability of the ex vivo expanded samples were significantly improved when compared with fresh samples . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
In 2 animals , cells of the different fractions were transduced in the presence of human FLT 3 ligand ( FLT3L ) , canine stem cell factor ( cSCF ) , and human megakaryocyte growth and development factor ( MGDF ) , and in 2 other dogs , transduction was performed in the presence of FLT3L , cSCF , and canine granulocyte colony stimulating factor ( cG CSF ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
The transduction protocol used the CH 296 recombinant human fibronectin fragment and relatively high concentrations of the flt 3 ligand and stem cell factor . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
The in vitro radiation sensitivity of CFU Meg isolated from human placental and umbilical cord blood was evaluated in plasma clot cultures stimulated by recombinant human cytokines , including thrombopoietin , the FLT 3 ligand ( FLT3LG ) , interleukin 3 , interleukin 11 and stem cell factor . ^^^ Treatment with thrombopoietin alone or in combination with FLT3LG and / or interleukin 11 gave exponential radiation survival curves ( D ( 0 ) for immature CFU Meg = 56 77 cGy , D ( 0 ) for mature CFU Meg = 86 cGy 1 . 12 Gy ) , while marked shoulders were observed on the survival curves for colonies supported by the combination of thrombopoietin , interleukin 3 and stem cell factor ( D ( 0 ) for immature CFU Meg = 89 98 cGy ; D ( 0 ) for mature CFU Meg = 1 . 25 1 . 31 Gy ) . ^^^ Our results showed that the immature CFU Meg were more radiosensitive than the mature CFU Meg and that the combination of cytokines , including thrombopoietin , interleukin 3 and stem cell factor , affected the radiation sensitivity of CFU Meg to the same extent as with thrombopoietin alone or in combination with FLT3LG and / or interleukin 11 . . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Cord blood CD34+ cells were transduced to high levels over 24 48 hr in the presence of Flt 3 ligand , stem cell factor , interleukin 3 , and interleukin 6 . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
A combination of stem cell factor , Flt 3 ligand and IL 7 was also able to support the proliferation and differentiation of the progenitors in a suspension culture . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
The highest yield of CD1a ( + ) / CD14 ( ) cells could be obtained with stem cell factor , transforming growth factor beta , tumor necrosis factor alpha , GM CSF , and FLT 3 ligand . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : CD 34 ( + ) PBPC were cultured in serum free medium comparing different ( n = 32 ) combinations of stem cell factor ( S ) , interleukin 1 ( 1 ) , interleukin 3 ( IL 3 ) ( 3 ) , interleukin 6 ( 6 ) , erythropoietin ( E ) , granulocyte colony stimulating factor ( G ) , granulate macrophage colony stimulating factor ( GM ) , daniplestim ( D , a novel IL 3 receptor agonist ) , and Flt 3 ligand ( FL ) under various culture conditions . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Synergistic combinations of cytokines including TPO , interleukin ( IL ) 1 , IL 3 , IL 11 , stem cell factor , and FLT 3 ligand induce the ex vivo expansion of colony forming unit MK progenitors and MKs from cytokine mobilized peripheral blood cells , bone marrow , and cord blood CD34+ cells . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
We demonstrate here that LC development from human CD34+ hemopoietic progenitor cells in response to TGF beta 1 costimulation ( basic cytokine combination GM CSF plus TNF alpha , stem cell factor , and Flt 3 ligand ) is associated with pronounced cell cluster formation of developing LC precursor cells . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Cells were prestimulated with flt 3 ligand , stem cell factor , and interleukin 3 and transduced on fibronectin . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
To delineate the mechanism by which NK cells begin to express different patterns of Ly 49 and CD94 / NKG2 molecules , we developed a clonal assay in which NK1 . 1 ( ) , IL 2 / IL 15 receptor beta+ NK precursors generated by culture of multipotential Lin ( ) , c kit+ progenitors in IL 7 , stem cell factor and flt 3 ligand are induced to differentiate into NK1 . 1+ , Ly49+ NK cells . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Furthermore , we have ruled out the contribution of 6 known early acting cytokines , including interleukin 3 , interleukin 6 , granulocyte macrophage colony stimulating factor , stem cell factor , flt 3 ligand , and thrombopoietin , to this SCEPF activity . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
We tested the ability of a recently cloned stem cell factor , Flt 3 ligand ( Flt3L ) , in combination with interleukin 15 ( IL 15 ) , to stimulate CB mononuclear cells ( MNCs ) to proliferate and differentiate into NK cells , in comparison with adult peripheral blood ( APB ) MNCs . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
New cytokine / chemokine mobilization regimens are under investigation such as combined rHuG CSF and rHu thrombopoietin , rHuG CSF and interleukin 3 , rHuG CSF and rHu stem cell factor , rHuG CSF and Flt 3 ligand , human macrophage inflammatory protein , interleukin 1 , and interleukin 8 . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
CD34 ( + ) cord blood ( CB ) cells were cultured in serum free medium containing stem cell factor , Flt 3 ligand , megakaryocyte growth and development factor , and granulocyte colony stimulating factor . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
DESIGN AND METHODS : AML blasts were maintained with or without cytokines ( stem cell factor , FLT 3 ligand and interleukin 3 ) for 48 hours , and successively infected with two spin infection cycles . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Flt 3 ligand ( FL ) is a cytokine that promotes the survival , proliferation , and differentiation of hematopoietic progenitors in synergy with other growth factors , such as stem cell factor . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
OBJECTIVE : We evaluated the frequency of hematopoietic progenitor cells ( HPC ) in CD 34 ( + ) CLA ( + ) ( cutaneous lymphocyte associated antigen ) and CD 34 ( + ) CLA ( ) cord blood cells , and followed cellular growth and HPC production during cultures in Flt 3 ligand , thrombopoietin , and stem cell factor ( FTS ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
METHODS : CD34+ cells were transduced with lentivectors encoding for GFP before or after 21 days of culture with FLT 3 ligand , thrombopoietin and stem cell factor and induction into DC with GM CSF+IL 4 ( G 4 ) or G4+TNF ( GT 4 ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
To assess the hematopoietic defect involved in PNH , CD34+ CD59+ ( normal phenotype hematopoietic stem / progenitor ) and CD34+ CD 59 ( PNH phenotype ) cells from PNH patients ( n = 16 ) and CD34+ CD59+ cells from healthy volunteers ( n = 10 ) were sorted as single cells into 96 well flat bottom culture plates containing culture medium supplemented with stem cell factor , interleukin ( IL ) 3 , erythropoietin , granulocyte macrophage colony stimulating factor ( GM CSF ) , G CSF , IL 6 , thrombopoietin , and Flt 3 ligand . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
The combination of thrombopoietin , Flt 3 ligand , and stem cell factor upregulated survivin expression in CD 34 ( + ) cells within 24 hours ; survivin expression was cell cycle related and highest during G2 / M , whereas growth factor withdrawal resulted in decreased survivin expression . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
CD34+ cells were cultured in 10 VIVO 10 supplemented with thrombopoietin , stem cell factor , and Flt 3 ligand . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
We examined the functional differences between G ( 0 ) and G ( 1 ) cord blood CD34+ cells for up to 24 weeks in serum free suspension culture , containing Flt 3 ligand , thrombopoietin and stem cell factor . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
METHODS : G CSF mobilized peripheral CD 34 ( + ) cells were cultured for 4 days with stem cell factor , flt 3 ligand , and thrombopoietin . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Apart from granulocyte macrophage CSF ( GM CSF ) , there have as yet been no reports of lithium salts inducing early activating factors for the megakaryocytic lineage , such as IL 3 , IL 11 , stem cell factor and flt 3 ligand , or maturation factors , such as thrombopoietin ( TPO ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
It could be increased in 5 7 days when the cells were cultured in vitro , especially in the presence of stem cell factor , interleukin 3 ( IL 3 ) , IL 6 and Flt 3 Ligand together ; the positive rate reached 48 % . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
On the other hand , SDF 1 significantly increased the viability of CD 34 ( + ) T cell precursors modulating the expression of BCL 2 and BAX genes , and stimulated the proliferation of CD 34 ( + ) thymic precursor cells , particularly in synergy with interleukin 7 ( IL 7 ) , but not with other cytokines , such as stem cell factor or flt 3 ligand . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
In this study , we investigated the effects of the cytokines flt 3 ligand , stem cell factor , and thrombopoietin on expansion of CD34+ and CD34+ / CD38 cells . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Here , we demonstrate the selective expansion of CD34+CD38 cells from purified CD34+ cells upon stimulation with Flt 3 ligand , stem cell factor and thrombopoietin . ^^^ We conclude that the combination Flt 3 ligand ( FL ) , stem cell factor and thrombopoietin ( TPO ) induced strong ex vivo proliferation of CD34+CD38 cells and that the absolute number of expanded cells with stem cell activity increased substantially in this population . . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
In the current study , we demonstrated that it is possible to harvest about 28 to 80 fold more veto cells on culturing of purified CD 34 ( + ) cells for 7 to 12 days with an early acting cytokine mixture including Flt 3 ligand , stem cell factor , and thrombopoietin . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
In the expansion group , CD34+ cells were cultured for 6 d with a combination of early acting cytokines ( Flt 3 ligand , stem cell factor , thrombopoietin and interleukin 3 ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Combinations of thrombopoietin and cytokines that act on megakaryocyte development ( stem cell factor , IL 3 , IL 6 , IL 11 , flt 3 ligand ( now known as FLT3LG ) , erythropoietin , GM CSF and G CSF were evaluated for their ability to enhance clonal growth in vitro of 10 irradiated CD 34 ( + ) megakaryocytic progenitor cells ( CFU megakaryocytes ) purified from normal human peripheral blood . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Residual CD34+ cells were successfully expanded in serum free medium in the presence of antiapoptotic cytokine combination ( stem cell factor + FLT 3 ligand + thrombopoietin + interleukin 3 , 50 ng / mL each , i . e . , 4F ) : KCD34+ = x2 . 8 and x13 . 7 ( n = 2 ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
During myeloid / monocytic differentiation induced by incubation with stem cell factor , thrombopoietin , and flt 3 ligand , partially differentiated HSCs emerge , which readily take up these pathogens and also latex beads by macropinocytosis . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Pure population of CD 34 ( + ) cells was incubated in a serum free medium supplemented with thrombopoietin , stem cell factor and Flt 3 ligand for 48 h and then transfected with plasmid DNA carrying the enhanced version of green fluorescent protein ( EGFP ) as a reporter gene . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
The cytokines included the recombinant human megakaryocyte growth and development ( 100 ng / ml ) , interleukin 3 ( 10 ng / ml ) , stem cell factor ( 100 ng / ml ) , flt 3 ligand ( 50 ng / ml ) and interleukin 11 ( 200 ng / ml ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
We investigated the role of three parameters : A ) the culture medium ( serum free versus serum dependent ) ; B ) the initial cell population ( Ficoll separated mononucleated cells versus CD 34 ( + ) selected cells ) , and C ) the low concentration of recombinant cytokines , flt 3 ligand , and thrombopoietin in association with a basic cocktail of stem cell factor , interleukin ( IL ) 6 , IL 3 , GM CSF , and erythropoietin . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
We replaced granulocyte macrophage colony stimulating factor with macrophage colony stimulating factor from a serum free culture containing granulocyte macrophage colony stimulating factor , stem cell factor , Flt 3 ligand , tumor necrosis factor alpha , and transforming growth factor beta 1 . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
After 1 week long in vivo treatment with various combinations of interleukin ( IL ) 15 , flt 3 ligand , stem cell factor , IL 2 , IL 12 , and megakaryocyte growth and differentiation factor , CD56+CD3 cells were detected in bone marrow ( BM ) , spleen , and peripheral blood ( PB ) , comprising 5 % to 15 % of human CD45+ cells . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
When cultivated in flt 3 ligand , stem cell factor and interleukin 7 ( IL 7 ) in a stroma free culture system MLL ENL transduced as well as control cells showed a wave of B lymphopoiesis . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
The cells were cultured in gas permeable Teflon coated bags in serum free medium containing the following cytokines : recombinant human interleukin 3 , recombinant human Flt 3 ligand , recombinant human stem cell factor , and recombinant human thrombopoietin . ^^^ MK precursors can be successfully expanded from CD 34 ( + ) UCB cells in a closed liquid culture system using interleukin 3 , recombinant human Flt 3 ligand , recombinant human stem cell factor , and recombinant human thrombopoietin to a level that should have a clinical impact in the transplantation setting . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Alteration of spontaneous proliferation by at least one mediator was detected in five of 38 patients ; whereas , altered cytokine ( Flt 3 ligand , granulocyte macrophage colony stimulating factor , stem cell factor ) dependant proliferation was observed for 10 patients . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Effects of a cytokine prestimulation period ( 18 hours ) with interleukin 3 , stem cell factor , Flt 3 ligand , and thrombopoietin were then analyzed in total cells , CFCs , and LTC ICs derived from mPB CD 34 ( + ) cells . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
CD34+ cell derived hematopoietic precursors amplified with FLT 3 ligand , thrombopoietin and stem cell factor became , after a 6 day induction with GM CSF , IL 4 and TGF beta 1 , HLA DR+ , CD1a+ , CD 83 , CD 86 , CD 80 cells . ^^^ Both CD1a CD 14 and CD1a CD14+ cells sorted from FLT 3 ligand , thrombopoietin and stem cell factor cultures responded to TNF alpha by an increase of Langerin+ cells . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NK cells are derived from hematopoietic stem cells , for which stem cell factor or Flt 3 ligand is required in the early stage of differentiation to NK cell progenitors . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Single administration of stem cell factor , FLT 3 ligand , megakaryocyte growth and development factor , and interleukin 3 in combination soon after irradiation prevents nonhuman primates from myelosuppression : long term follow up of hematopoiesis . ^^^ The present study investigated the hematopoietic response of cynomolgus monkeys to a single dose of stem cell factor , FLT 3 ligand , megakaryocyte growth and development factor , and interleukin 3 in combination ( 4F , each factor given intravenously at 50 microg / kg ) administered 2 hours after 5 Gy gamma irradiation . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
The speed of recovery of CD11c ( + ) CD 123 ( low ) DC 1 exceeded that of CD11c ( ) CD 123 ( + ) DC 2 , and correlated with plasma levels of flt 3 ligand ( FL ) , but not with granulocyte or granulocyte macrophage colony stimulating factors and stem cell factor . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
In culture with stem cell factor , FLT 3 ligand , and IL 6 , a 4 hydroxytamoxifen inducible form of c Myc ( Myc / ERT ) enabled murine Lin ( ) Sca 1 ( + ) HSCs to proliferate with the surface phenotype compatible with HSCs for more than 28 days . c Myc activated by 4 hydroxytamoxifen augmented telomerase activities and increased the number of CFU Mix about 2 fold in colony assays . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Cord blood derived purified CD34+ cells were grown in liquid medium supplemented with the cytokines stem cell factor , thrombopoietin , Flt 3 ligand , and IL 6 , and the chelator TEPA for the first 3 weeks and then for up to 11 weeks with cytokines alone . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Finally , in vitro proliferation , but not differentiation , of CD34+ cells cultured in the presence of fms like tyrosine kinase 3 ligand ( FLT3L ) , stem cell factor , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 , and G CSF is profoundly inhibited by treatment with CsA in a dose dependent manner . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Here , we describe the characterization of purified CD 133 ( + ) progenitor cells from umbilical cord blood , a subset of CD 34 ( + ) hematopoietic progenitors that were grown in proliferation medium containing Flt 3 ligand , thrombopoietin and stem cell factor . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
METHODS : Enriched CD34+ cells were cultured for 8 or 10 d in serum free media ( QBSF 60 or 10 Vivo 10 ) and four combinations of cytokines consisting of recombinant human pegylated megakaryocyte growth and development factor , stem cell factor , flt 3 ligand , G CSF , interleukin ( IL ) 6 , platelet derived growth factor ( PDGF ) , and IL 1beta . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
BM mononuclear cells from 3 dogs with melanoma and from 1 healthy dog were cultured for 12 days in media supplemented with recombinant human granulocyte macrophage colony stimulating factor , stem cell factor , tumor necrosis factor , and Flt 3 ligand . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Notably , valproic acid highly preserves the CD 34 positivity after 1 week ( range , 40 89 % ) or 3 weeks ( range , 21 52 % ) amplification cultures with two ( Flt3L + thrombopoietin ) or four cytokines ( Flt3L + thrombopoietin + stem cell factor + interleukin 3 ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
However , when bone marrow ( BM ) progenitors from IL 15Ralpha ( / ) mice were cultured with IL 7 , stem cell factor and flt 3 ligand , followed by IL 15 , they were able to differentiate into functional NK cells , indicating that IL 15Ralpha is not critical for NK cell development . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : HSC were enriched from steady state bone marrow by magnetic cell sorting ( lineage depletion ) and cultured in defined serum free medium containing an improved growth factor cocktail ( Flt 3 ligand , stem cell factor , interleukin 3 , interleukin 11 ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Cord blood derived lin ( ) cells were cultured for 8 days in serum free medium supplemented with stem cell factor , Flt 3 ligand , and thrombopoietin . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
UCB CD34+ cells were cultured for 10 days in serum free medium supplemented with recombinant human thrombopoietin , stem cell factor , flt 3 ligand and interleukin 3 . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
This method is based on immunoselection of CD34+ cells derived from mobilized peripheral blood of patients with metastatic breast cancer and expansion in the presence of flt 3 ligand , stem cell factor , interleukin 6 , and thrombopoietin . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Subsequently , we tested the reconstituting potential of cells cultured in FGF 1 + 2 with the addition of early acting stimulatory molecules , stem cell factor +interleukin 11 + Flt 3 ligand . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
At doses that abrogated megakaryocytopoiesis , HC also inhibited the expansion of CD 34 ( + ) cells stimulated by stem cell factor , interleukin 3 and Flt 3 ligand and also induced apoptosis . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Finally , reverse transcriptase polymerase chain reaction analysis showed that UC MSC had a cytokine spectrum very similar to that of BM MSC , including expression of the mRNA of stem cell factor , leukemia inhibitor factor , macrophage colony stimulating factor , Flt 3 ligand , interleukin 6 , vascular endothelial growth factor and stromal derived factor 1 , but UC MCS additionally expressed mRNA of granulocyte macrophage and granulocyte colony stimulating factors . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
This study examined the effects of mesenchymal stem cells ( MSCs ) on the maintenance of CD34+ cells driven by aza D and TSA in culture with the combined cytokines of thrombopoietin , flt 3 ligand , stem cell factor , interleukin 3 , and interleukin 6 . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
In media supplemented with thrombopoietin , stem cell factor , flt 3 ligand , and granulocyte colony stimulating factor , CD34+ cells , as well as CFU GM , BFU CFU E , CFU GEMM , and CFU MK , increased by 34 . 3 , 138 , 59 . 7 , 38 . 4 , and 86 . 0 fold , respectively . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
When CD36+ erythroid precursor cells , generated from CD34+ cells with stem cell factor , flt 3 ligand , thrombopoietin , interleukin 3 , interleukin 6 , and erythropoietin , were cultured with an HDAC inhibitor FK 228 ( depsipeptide ) at a specified dose in the presence of erythropoietin , their differentiation was inhibited , as determined by the expression of CD 45 and glycophorin A . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
We found that hTERT was incapable of prolonging the replicative capacity of CB cells maintained under serum free conditions in the presence of stem cell factor , Flt 3 ligand , thrombopoietin , and interleukin 3 beyond 4 months ( n=3 ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Therefore , administration of antiapoptotic cytokine combinations such as stem cell factor , Flt 3 ligand , thrombopoietin , and interleukin 3 ( 4F ) , may be important for multilineage recovery , particularly when these factors are administered early . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
A variety of factors produced by stromal fibroblasts , including Flt 3 ligand ( FL ) , interleukin 11 ( IL 11 ) , Steel factor ( SF ) , and IL 7 , have been implicated in stimulating the production of pre B cells and myeloid cells from primitive hematopoietic precursors . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Analysis of the mitogenic activity of interleukin 3 ( IL 3 ) , Steel factor ( SF ) , and flt 3 ligand ( FL ) on acute myelogenous leukemia ( AML ) blasts using the short term endpoints of proliferation in 3H thymidine ( 3H Tdr ) incorporation assays or methylcellulose cultures ( colony assays ) showed that greater than 90 % of samples contained cells that were responsive to one or more of these cytokines . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Time course studies revealed that the combination of Flt 3 ligand ( FL ) , Steel factor ( SF ) and interleukin 3 ( IL 3 ) did not elicit as large an amplification of the long term culture initiating cell ( LTC IC ) population in serum free cultures of CD34+ CD 38 cord blood ( CB ) cells as was obtained in similar cultures of adult human CD34+ CD 38 bone marrow ( BM ) cells ( 4 5 90 fold maximum increases ) , even though both total and colony forming cell ( CFC ) numbers initially increased more rapidly in CB cultures . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Recent studies have identified thrombopoietin ( TPO ) , flt 3 ligand ( FL ) , Steel factor ( SF ) , and interleukin 11 ( IL 11 ) as cytokines able to stimulate amplification of the most primitive murine hematopoietic cells in vitro . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
In the present study , we investigated whether 10 irradiated hematopoietic stem cells can be induced to undergo megakaryocytopoiesis and thrombopoiesis in vitro using cytokine combinations that have been demonstrated to be effective for conferring increased survival on irradiated human CD 34 ( + ) megakaryocytic progenitor cells ( colony forming unit megakaryocytes ; CFU Meg ) , such as thrombopoietin ( TPO ) , interleukin 3 ( IL 3 ) , stem cell factor and FLT 3 ligand . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Analysis of the effects of 16 cytokines on CD34+CD38 cells in the latter type of culture showed that Flt 3 ligand ( FL ) , Steel factor ( SF ) , and interleukin ( IL ) 3 were both necessary and sufficient to obtain an approximately 30 fold amplification of the input LTC IC population within 10 d . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Previous studies have shown that primitive human hematopoietic cells detectable as long term culture initiating cells ( LTC ICs ) and colony forming cells ( CFCs ) can be amplified when CD 34 ( + ) CD 38 ( ) marrow cells are cultured for 10 days in serum free medium containing flt 3 ligand ( FL ) , Steel factor ( SF ) , interleukin ( IL ) 3 , IL 6 , and granulocyte colony stimulating factor . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
In a different series of studies , after 5 week culture on human BM stromal cell line L87 / 4 layers , cells were forced to selected lineage differentiation by culture in the presence of low concentrations of SF and high concentrations of lineage specific cytokines such as Flt 3 ligand ( myeloid and pre B cell differentiation ) , Tpo ( megakaryocytic differentiation ) . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
Interestingly , after 72 hours in serum free culture with or without Steel factor ( SF ) , Flt 3 ligand ( FL ) , and interleukin 3 ( IL 3 ) , most G ( 0 ) AML cells entered active cell cycle ( percentage of AML cells remaining in G ( 0 ) at 72 hours , 1 . 2 % to 37 % , and 0 % to 7 . 6 % in cultures without and with growth factors [ GFs ] , respectively ) while G ( 0 ) cells from normal lineage depleted bone marrow remained quiescent in the absence of GF . ^^^
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P49771	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :1.202789
These results demonstrate that soluble flt3L interacts with membrane bound SCF in supporting the long term growth of bone marrow progenitor cells . 1.202789^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
In methylcellulose cultures , flt3L stimulated formation of hematopoietic colonies only weakly , whereas it had an additive effect when combined with erythropoietin ( Epo ) , stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , interleukin 11 ( IL 11 ) , or granulocyte colony stimulating factor ( G CSF ) . ^^^ Flt3L was less effective than SCF and did not further enhance the number of hematopoietic colonies formed in response to SCF containing combinations of multiple cytokines . ^^^ In long term liquid suspension cultures , flt3L was less mitogenic than SCF but its effect on the maintenance of progenitors was superior that of SCF and of IL 3 , IL 11 , and G CSF . ^^^ The in vitro effects of flt3L on AA cells differ in many aspects from those of the structurally related cytokine SCF , suggesting a benefit in use of a combination of these two early acting growth factors . . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
We compared two combinations of cytokines : interleukin 1alpha ( IL1alpha ) and interleukin 3 ( IL 3 ) with either Flt 3 ligand ( FLT3L ) or stem cell factor ( SCF ) . ^^^ Moreover , after 5FU pretreatment , the mean ( + / SEM ) percentage of Ph negativity was significantly greater for IL1alpha IL 3 FLT3L compared to IL1alpha IL 3 SCF ( 19 . 1 + / 2 . 5 % 5 14 . 8 + / 2 . 3 % , P=0 . 009 , n = 7 ) . ^^^ Thus , a subset of Ph negative cells was selected from CML PB by 5FU and expanded using the combination of cytokines IL1alpha IL 3 FLT3L and IL1alpha IL 3 SCF . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
The ligand for flt 3 ( FLT3L ) exhibits striking structural homology with stem cell factor ( SCF ) and monocyte colony stimulating factor ( M CSF ) and also acts in synergy with a range of other hematopoietic growth factors ( HGF ) . ^^^ To investigate the basis for the capacity of FLT3L to augment the de novo generation of myeloid progenitors from CD34+CD38 cells , single bone marrow CD34+CD38 cells were sorted into Terasaki wells containing serum free medium supplemented with interleukin 3 ( IL 3 ) , IL 6 , granulocyte colony stimulating factor ( G CSF ) , SCF ( 4 HGF ) + / FLT3L . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
We compared different combinations of cytokines containing interleukin 1 alpha ( IL 1 ) , interleukin 3 ( IL 3 ) , stem cell factor ( SCF ) , leukemia inhibitor factor ( LIF ) , Flt 3 ligand ( FLT3L ) , and thrombopoietin ( TPO ) . 5FU decreased cell proliferation in the liquid culture but concurrently increased the expansion of CFU GM . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
CD34+Thy1+ , CD34+Thy1 , CD34+38 , CD34+38+ , CD34+DR+ , CD34+DR , CD34+Rhodamine123 ( Rh 123 ) and CD34+Rh123+ cells were incubated for 7 days in IMDM + 10 % FCS + IL 3 + IL 6 + G CSF + SCF ( 36GS ) + flt3L . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
The relative effects of the cytokines M CSF , SCF , and Flt3L on the proliferation and development of the late myeloid progenitors granulocyte macrophage colony forming cells ( GM CFC ) were investigated . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Whilst samples without a mutation of the Flt 3 gene showed an increased cell production in response to either IL 3 and G CSF or IL 6 , SCF , TPO and Flt3L in long term stroma supported cultures , mutant samples failed to do so . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
To delineate factors involved in NK cell development , we established an in vitro system in which lineage marker ( Lin ) , c kit+ , Sca2+ bone marrow cells differentiate into lytic NK1 . 1+ but Ly 49 cells upon culture in IL 7 , stem cell factor ( SCF ) , and flt 3 ligand ( flt3L ) , followed by IL 15 alone . ^^^ A comparison of the ability of IL 7 , SCF , and flt3L to generate IL 15 responsive precursors suggested that NK progenitors express the receptor for flt3L . ^^^ To test the hypothesis that additional marrow derived signals are necessary for Ly 49 expression on developing NK cells , flt3+ progenitors were grown in IL 7 , SCF , and flt3L followed by culture with IL 15 and a marrow derived stromal cell line . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
In 2 animals , cells of the different fractions were transduced in the presence of human FLT 3 ligand ( FLT3L ) , canine stem cell factor ( cSCF ) , and human megakaryocyte growth and development factor ( MGDF ) , and in 2 other dogs , transduction was performed in the presence of FLT3L , cSCF , and canine granulocyte colony stimulating factor ( cG CSF ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
We describe here that lineage phenotype negative ( Lin ) ( ) c kit ( + ) hematopoietic progenitor cells ( HPCs ) from day 13 postcoitus ( dpc ) murine fetal liver ( FL ) can generate dendritic cell ( DC ) precursors when cultured in vitro in the presence of PA 6 stromal cells plus granulocyte / macrophage colony stimulating factor ( GM CSF ) + stem cell factor ( SCF ) + Flt 3 ligand ( Flt3L ) for 12 to 14 days , and develop into mature DCs when stimulated with GM CSF plus mouse tumor necrosis factor alpha ( mTNFalpha ) for an additional 3 to 5 days . ^^^ Once FL derived HPCs were cultured without GM CSF , NK1 . 1 ( + ) cells developed in the presence of PA 6 cells + SCF + Flt3L . ^^^ These NK1 . 1 ( + ) cells could develop into DC precursors at an earlier stage of differentiation by reculturing with PA 6 cells + SCF + Flt3L + GM CSF , but they would be irreversibly committed to NK cell precursors without GM CSF after 3 days , suggesting that GM CSF plays a critical role in controlling the transition of DC and NK cell precursors from 13 dpc FL derived Lin ( ) c kit ( + ) HPCs . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Comparisons of structure function relationships of Flt3L with the homologous hematopoietic cytokines macrophage colony stimulating factor ( MCSF ) and stem cell factor ( SCF ) suggest that they have a common receptor binding mode that is distinct from the paradigm derived from the complex of growth hormone with its receptor . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
By day 21 , the endothelial based system receiving the FLT3L , SCF , IL 6 , and GM CSF cytokine combination contained five fold greater numbers of CD34+ than the stroma cell free culture cell system . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
We tested the ability of a recently cloned stem cell factor , Flt 3 ligand ( Flt3L ) , in combination with interleukin 15 ( IL 15 ) , to stimulate CB mononuclear cells ( MNCs ) to proliferate and differentiate into NK cells , in comparison with adult peripheral blood ( APB ) MNCs . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
The cytokines FLT3L + SCF + IL 7 + IGF 1 were added at day 0 , IL 4 + IL 5 + IL 6 + IL 10 and soluble CD 40 ligand at day 14 , and Staph . aureus Cowan particles on day 21 . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Blast cells were expanded in serum free medium supplemented with Flt3L , G CSF , GM CSF , IL 3 , IL 6 and SCF for 7 days and subsequently stimulated with Flt3L , GM CSF and TGF beta for a further 14 days , with the addition of TNF alpha for the final 48 h of culture . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
CD34+ cells from cord blood ( CB ) , bone marrow ( BM ) , or peripheral blood ( PB ) were expanded in a medium containing stem cell factor ( SCF ) , flt 3 ligand ( Flt3L ) and thrombopoietin ( TPO ) with or without HESS 5 , a murine BM stromal cell line , for 2 weeks ( the first expansion step ) , then differentiated to DC in a medium containing granulocyte macrophage colony stimulating factor ( GM CSF ) , flt 3 ligand ( Flt3L ) , stem cell factor ( SCF ) , tumor necrosis factor alpha ( TNF alpha ) , IL 4 , and lipopolysaccharide ( LPS ) for 9 days ( the second differentiation step ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
We cultured HPCs in a serum free medium in the presence of IL 3 and a combination cytokines including Flt3L , SCF , and TNF alpha with or without TGF beta 1 . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Specifically , TNF alpha is a potent inhibitor of the clonal growth of primitive and committed murine and human bone marrow progenitors in combination with multiple cytokines , including granulocyte colony stimulating factor ( G CSF ) , CSF 1 , erythropoietin ( Epo ) , stem cell factor ( SCF ) , and flt 3 ligand ( FL ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Among this group are Steel factor ( also known as mast cell growth factor , stem cell factor and kit ligand ) , and the more recently described flt 3 ligand . ^^^ The flt 3 ligand : a hematopoietic stem cell factor whose activities are distinct from steel factor . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
In previous studies using stroma free short term assays , Flt 3 ligand ( FL ) has been shown to induce proliferation of HPC at proportions similar to or less than c kit ligand ( steel factor , SF ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Stem cell factor , but not flt 3 ligand , induces differentiation and activation of human mast cells . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Finally , in vitro proliferation , but not differentiation , of CD34+ cells cultured in the presence of fms like tyrosine kinase 3 ligand ( FLT3L ) , stem cell factor , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 , and G CSF is profoundly inhibited by treatment with CsA in a dose dependent manner . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
While hematopoietic development was diminished without serum , here we demonstrate with the stromal cell coculture model that addition of the growth factors stem cell factor ( SCF ) , thrombopoietin ( TPO ) , and Flt 3 ligand ( Flt3L ) to serum free media does allow isolation of hematopoietic progenitors . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Notably , valproic acid highly preserves the CD 34 positivity after 1 week ( range , 40 89 % ) or 3 weeks ( range , 21 52 % ) amplification cultures with two ( Flt3L + thrombopoietin ) or four cytokines ( Flt3L + thrombopoietin + stem cell factor + interleukin 3 ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Because hESCs express growth factor receptors , including those for basic fibroblast growth factor ( bFGF ) , stem cell factor ( SCF ) , and fetal liver tyrosine kinase 3 ligand ( Flt3L ) , we evaluated these and other growth factors for their ability to maintain undifferentiated hESCs in the absence of conditioned medium . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
OBJECTIVE : To evaluate canine histiocytic sarcoma cell lines and tumor samples for dysregulation of the Kit / stem cell factor ( SCF ) , Flt3 / Flt3 ligand ( Flt3L ) , and Met / hepatocyte growth factor ( HGF ) receptor tyrosine kinase signaling pathways , as these are known to contribute to the differentiation and survival of normal dendritic cells as well as malignant transformation of dendritic cells in mouse models . ^^^ PROCEDURE : Histiocytic sarcoma cell lines were evaluated for expression of Kit / SCF , Flt3 / Flt3L , and Met / HGF by use of reverse transcriptase PCR procedures . ^^^ CONCLUSIONS AND CLINICAL RELEVANCE : These data suggest that dysregulation of Kit / SCF , Flt3 / Flt3L , and Met / HGF signaling pathways is unlikely to occur in histiocytic sarcomas of dogs and that inhibitors of the Kit , Flt 3 , and Met pathways are unlikely to provide clinical benefit to dogs with histiocytic sarcomas . . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
We have performed a comprehensive analysis of cell lines and tissues to compare and contrast the expression patterns of Flt 3 ligand ( FL ) , c Kit ligand ( KL ) , and macrophage colony stimulating factor as well as their receptors , Flt 3 , c Kit , and c Fms . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
However , the more restricted cellular distribution of the FLT 3 receptor may target the effects of FLT 3 ligand to primitive hematopoietic cells and to myeloid and lymphoid progenitor cells , in contrast to the pleiotropic effects of the c kit receptor ligand , stem cell factor . . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
In quiescent HPCs the receptors for early acting [ flt 3 ligand ( FL ) , c kit ligand ( KL ) , bFGF , interleukin 6 ( IL 6 ) ] and multilineage [ IL 3 , granulocyte macrophage CSF ( GM CSF ) ] HGFs are expressed at significant levels but with different patterns , eg , kit and flt 3 are detected on a majority and minority of HPCs , respectively , whereas IL 3Rs and GM CSFRs are present on almost all HPCs . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
In this study , the effects of c kit ligand ( KL ) and flt 3 ligand ( FL ) were compared in the presence of IL 3 , GM CSF , and erythropoietin ( 3 / GM / EPO ) , using frequent medium exchange cultures of human bone marrow mononuclear cells ( BMMNC ) and CD 34 enriched cells . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Using a plasma clot clonal assay and a long term bone marrow culture ( LTBMC ) system , we studied the effects of the recently cloned human flt 3 ligand ( FL ) alone and in combination with granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) , or stem cell factor ( c kit ligand [ KL ] ) on human megakaryocytopoiesis . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Incubation with single recombinant cytokines from a broad spectrum of growth factors established that the strongest proliferation response of MUTZ 2 cells was elicited by FLT 3 ligand , granulocyte colony stimulating factor ( G CSF ) , macrophage CSF ( M CSF ) , interferon gamma ( IFN gamma ) and stem cell factor ( SCF ) , whereas granulocyte macrophage CSF ( GM CSF ) , M CSF , interleukin 3 ( IL 3 ) and SCF were the most effective growth factors in inducing proliferation of MUTZ 3 . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Analysis of the effects of 16 cytokines on CD34+CD38 cells in the latter type of culture showed that Flt 3 ligand ( FL ) , Steel factor ( SF ) , and interleukin ( IL ) 3 were both necessary and sufficient to obtain an approximately 30 fold amplification of the input LTC IC population within 10 d . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
In the present study , the flt 3 ligand ( FL ) , which , in combination with other cytokines , has overlapping activities with SCF on myeloid cell production from uncommitted progenitors , was investigated for its ability to induce selective stroma independent B cell commitment from uncommitted Lin Sca 1+ bone marrow progenitor cells . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
All cases were also tested for their proliferative response to human FLT 3 ligand ( FL ) and KIT ligand ( KL ) and for colony formation in the presence of single or associated cytokines . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Finally , the effect of IL 3 , IL 6 , SCF , and Flt 3 ligand ( FL ) on cycle induction was examined . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
In the first step , cells received a primary stimulation consisting of either stem cell factor ( SCF ) , Flt 3 ligand ( FL ) , interleukin 3 ( IL 3 ) , or IL 6 for 7 days . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
LN provirus was detected by polymerase chain reaction in the marrow recovered from 9 of 10 mice transplanted with human CD34+ cells transduced with stromal support , 5 of 11 mice that received human cells transduced in suspension culture with FL , but none of the 10 mice that received human cells transduced in suspension culture without FL We conclude that FLT 3 ligand , in conjunction with IL 3 , IL 6 , and SCF , preserves the generative capacity of primitive human hematopoietic cells during in vitro transductions in suspension culture . . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Coexpression of flt 3 ligand / flt 3 and SCF / c kit signal transduction system in bile duct ligated SI and W mice . ^^^ It has been reported that the flt 3 ligand ( FL ) / flt 3 system can synergize with the SCF / c kit system and stimulate the proliferation of hematopoietic cells . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
We studied the effects of stem cell factor ( SCF ) and flt 3 ligand ( FL ) on the ex vivo expansion of human umbilical cord blood ( CB ) derived CD34+ cells in combination with various cytokines , including interleukin ( IL ) 3 , IL 6 , IL 11 , and c Mpl ligand ( thrombopoietin , TPO ) , in a short term serum free liquid suspension culture system . ^^^ Thrombopoietin augments ex vivo expansion of human cord blood derived hematopoietic progenitors in combination with stem cell factor and flt 3 ligand . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
MGDF also enhanced interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , c kit ligand ( KL ) and FLT 3 ligand ( FL ) stimulated blast cell proliferation . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Previous studies have shown that primitive human hematopoietic cells detectable as long term culture initiating cells ( LTC ICs ) and colony forming cells ( CFCs ) can be amplified when CD 34 ( + ) CD 38 ( ) marrow cells are cultured for 10 days in serum free medium containing flt 3 ligand ( FL ) , Steel factor ( SF ) , interleukin ( IL ) 3 , IL 6 , and granulocyte colony stimulating factor . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Differential effects of Flk 2 / Flt 3 ligand and stem cell factor on murine thymic progenitor cells . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
In a different series of studies , after 5 week culture on human BM stromal cell line L87 / 4 layers , cells were forced to selected lineage differentiation by culture in the presence of low concentrations of SF and high concentrations of lineage specific cytokines such as Flt 3 ligand ( myeloid and pre B cell differentiation ) , Tpo ( megakaryocytic differentiation ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Tpo alone stimulated limited clonal growth , but synergized with c kit ligand ( KL ) , flt 3 ligand ( FL ) , or IL 3 to potently enhance clonogenic growth . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Flt 3 ligand ( FL ) shares many features with stem cell factor ( SCF ) , a widely documented cofactor for peripheral blood progenitor cell ( PBPC ) mobilization . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
A numerical expansion of stem cell subsets was observed in the presence of interleukin 3 ( IL 3 ) , stem cell factor , and IL 6 , while stroma contact , stromal soluble factors , or combined addition of FLT 3 ligand and thrombopoietin improved this parameter . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
A variety of factors produced by stromal fibroblasts , including Flt 3 ligand ( FL ) , interleukin 11 ( IL 11 ) , Steel factor ( SF ) , and IL 7 , have been implicated in stimulating the production of pre B cells and myeloid cells from primitive hematopoietic precursors . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Analysis of the mitogenic activity of interleukin 3 ( IL 3 ) , Steel factor ( SF ) , and flt 3 ligand ( FL ) on acute myelogenous leukemia ( AML ) blasts using the short term endpoints of proliferation in 3H thymidine ( 3H Tdr ) incorporation assays or methylcellulose cultures ( colony assays ) showed that greater than 90 % of samples contained cells that were responsive to one or more of these cytokines . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Low proliferative capacity was observed after 1 week in culture in the presence of stem cell factor ( SCF ) + Flt 3 ligand ( FL ) + G CSF . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Culture of lineage marker ( Lin ) , c kit+ , Sca2+ , interleukin ( IL ) 2 / 15Rbeta ( CD 122 ) marrow cells in IL 6 , IL 7 , stem cell factor ( SCF ) , and flt 3 ligand ( flt 3 L ) for 5 6 d followed by IL 15 alone for an additional 4 5 d expanded the starting population 30 40 fold and gave rise to a virtually pure population of NK1 . 1+ , CD 3 cells . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Similar enhancement was seen when combinations of cytokines , ie , G CSF plus kit ligand or G CSF plus Flt 3 ligand were used with anti VLA 4 in primates and mice . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
In an effort to obtain human B lymphoid , as well as T lymphoid and myeloid cell development in the mice , CD34+ cells were coinjected with human marrow stromal cells engineered to secrete human IL 2 , IL 7 , stem cell factor or FLT 3 ligand , + / IL 3 . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
This population was then used to evaluate the effects of supplemental cytokines ( interleukin 2 [ IL 2 ] , IL 3 , IL 7 , c kit ligand ) , FLT 3 ligand ( FL ) , and stroma derived factors on lymphoid differentiation in vitro . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Cell numbers in the suspension cultures initiated with Flt3high cells were maintained in the presence of Flt 3 ligand ( FL ) alone , and increased in response to FL plus kit ligand ( KL ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Distinct actions of Flt 3 ligand and stem cell factor on myeloid lineage selection and maturation of granulocytes versus macrophages . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
To address this issue , we stimulated purified candidate HSC from human adult bone marrow with stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , and Flt 3 ligand ( FL ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
The number of primitive human hematopoietic long term culture initiating cell derived colonies ( LTC IC derived colonies ) generated from CD34+ and CD34+ / HLA DRlo peripheral blood progenitor `` stem ' ' cells ( PBSCs ) increased during 7 days of treatment in this vector containing medium in the presence of IL 3 , SCF , and flt 3 ligand . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Flt 3 ligand , MGDF , Epo and G CSF enhance ex vivo expansion of hematopoietic cell compartments in the presence of SCF , IL 3 and IL 6 . ^^^ The aim of the study is to define the ability of Flt 3 ligand , MGDF , Epo and G CSF to modulate the expansion of different hematopoietic compartments in association with a basic cocktail of SCF + IL 3 + IL 6 ( S 36 ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Cells with a dendritic morphology and expressing HLA DR , CD1a , S 100 and CD 83 were maximally expanded under serum free conditions with the addition of SCF , GM CSF , TNF alpha , TGF beta and Flt 3 ligand ( fold increase of CD1a+ cells = 102 + / 32 after 2 weeks of culture ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Addition of FLT 3 ligand to IL 2 , IL 7 SCF , and stromal factors are important in early stages of NK development . . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
We analyzed the effect of tumor necrosis factor ( TNF ) alpha on the differentiation and viability of dendritic cells ( DC ) generated from cord blood CD34+ progenitors cultured for five days with GM CSF , Flt 3 ligand ( FL ) , and stem cell factor ( SCF ) , and then with GM CSF only [ TNF ( ) cultures ] . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
RT PCR analysis of cytokine and growth factor mRNA in MSCs and MDSCs revealed a very similar pattern of mRNAs including IL 6 , 7 , 8 , 11 , 12 , 14 , and 15 , M CSF , Flt 3 ligand , and SCF . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
We compared the effect of human flt 3 ligand ( FL ) and stem cell factor ( SCF ) on cord blood ( CB ) derived CD34+ cells expressing different levels of flt 3 or c kit tyrosine kinase ( TK ) receptor in clonal cell culture . ^^^ Haematopoietic action of flt 3 ligand on cord blood derived CD 34 positive cells expressing different levels of flt 3 or c kit tyrosine kinase receptor : comparison with stem cell factor . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
We next compared the activation kinetics of individually sorted G0CD34+ cells stimulated with stem cell factor ( SCF ) , flt 3 ligand ( FL ) , or interleukin 3 ( IL 3 ) as single factors . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Newer growth factors such as stem cell factor , thrombopoietin , Flt 3 ligand , and interleukins have shown promising results in the laboratory , and some have been used in clinical trials . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
However , ex vivo expansion of the sorted subsets with interleukin 3 , stem cell factor and FLT 3 ligand for 2 weeks resulted in a significant production of O 2 and H2O2 / HOCl by CD34+ / CD117low cell fraction , compared to the same sorted but not expanded counterparts . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Time course studies revealed that the combination of Flt 3 ligand ( FL ) , Steel factor ( SF ) and interleukin 3 ( IL 3 ) did not elicit as large an amplification of the long term culture initiating cell ( LTC IC ) population in serum free cultures of CD34+ CD 38 cord blood ( CB ) cells as was obtained in similar cultures of adult human CD34+ CD 38 bone marrow ( BM ) cells ( 4 5 90 fold maximum increases ) , even though both total and colony forming cell ( CFC ) numbers initially increased more rapidly in CB cultures . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Supernatants of IL 1alpha stimulated EC potentiated the effects of an association of IL 1 , IL 3 , IL 6 , LIF , SCF , Flt 3 ligand , TPO , G CSF , GM CSF , M CSF and IL 11 on the proliferation of hematopoietic progenitors suggesting that EC may produce other soluble growth factors potentiating the action of the above set of cytokines . . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Of the two hematopoietic growth factors , acting at the early stages of hematopoiesis , circulating levels of flt 3 ligand are highly elevated in patients with aplastic anemia , whereas those of stem cell factor ( SCF ) are essentially normal . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Cord blood CD34+ cells were cultured with granulocyte macrophage colony stimulating factor ( GM CSF ) and tumor necrosis factor alpha ( TNF alpha ) , to which stem cell factor and Flt 3 ligand were added for 5 days . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Improved gene transfer into baboon marrow repopulating cells using recombinant human fibronectin fragment CH 296 in combination with interleukin 6 , stem cell factor , FLT 3 ligand , and megakaryocyte growth and development factor . ^^^ The highest level of gene transfer was obtained after 24 hours of prestimulation followed by 48 hours of incubation on CH 296 in vector containing medium in the presence of multiple hematopoietic growth factors ( interleukin 6 , stem cell factor , FLT 3 ligand , and megakaryocyte growth and development factor ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
CD34+ cells purified from leukapheresis product were seeded at 1 10 10 ( 4 ) cells / mL in complete medium supplemented with GM CSF , TNF alpha , IL 4 , c kit ligand , and flt 3 ligand ( FL ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Circulating CD34+ cell populations characterized by a low rate ( up to five ) or high rate ( more than five ) of cell divisions were isolated from 8 d cultures in the presence of stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , granulocyte colony stimulating factor ( G CSF ) , erythropoietin ( EPO ) , Flt 3 ligand and Peg rHu megakaryocyte growth and development factor ( Peg rHuMGDF ) using the fluorescent dye 5 , 6 carboxyfluorescein diacetate succinimidyl ester ( CFDA SE ) and flow cytometric cell sorting . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Freshly isolated G0CD34 ( + ) cells were activated by interleukin 3 ( IL 3 ) , stem cell factor ( SCF ) , and flt 3 ligand ( FL ) for a 36 hour incubation period during which a fraction of cells progressed from G 0 into G 1 but did not complete a cell cycle . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
By reverse transcription polymerase chain reaction , the expression by MMHs of cytokines involved in the survival and self renewal of early progenitor cells ( stem cell factor and FLT 3 ligand ) as well as those acting at different stages of progenitor differentiation ( interleukin [ IL ] 1beta , IL 3 , leukemia inhibitory factor , IL 6 , granulocyte macrophage colony stimulating factor , granulocyte colony stimulating factor , macrophage colony stimulating factor , and thrombopoietin ) was shown . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
When combinations of cytokines were used over 7 days , between 5 % ( FLT 3 ligand [ FLT 3L ] , stem cell factor [ SCF ] , IL 3 , IL 6 , granulocyte colony stimulating factor [ G CSF ] , beta nerve growth factor [ betaNGF ] ) and 22 % ( FLT 3L + HGF ) of the cells remained undivided , between 15 % ( HGF , IL 1 , IL 11 , G CSF ) and 68 % ( SCF + TPO ) of the cells had doubled and between 27 % ( FLT 3L + TPO ) and 70 % ( HGF , IL 1 , IL 11 , G CSF ) died . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
The culture system features the use of a serum free medium supplemented with a cocktail of cytokines including flt 3 ligand , interleukin 3 ( IL 3 ) , stem cell factor ( SCF ) , and IL 2 . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Other mouse models have indicated an unexpected role for flt 3 ligand in early NK cell development as well as minor roles for stem cell factor and IL 7 in expanding NK cell progenitor numbers . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Progenitors were cultured in limiting dilutions with interleukin 7 ( IL 7 ) , flt 3 ligand ( FL ) , c kit ligand ( KL ) , IL 3 , IL 2 , and AFT 024 , a murine fetal liver line , which supports culture of transplantable murine stem cells . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
The results show that using a growth factor combination including granulocyte macrophage colony stimulating factor plus tumor necrosis factor alpha plus interleukin 4 plus stem cell factor plus Flt 3 ligand , more than 70 % of DCs derived from CB or MPB CD34+ cells can be transduced . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NB from four infants was expanded by culturing the blood samples in the presence of thrombopoietin ( Tpo ) , interleukin 1beta ( IL1beta ) , IL 3 , IL 6 , flt 3 ligand and stem cell factor ( SCF ) for 7 d . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Purified human CD34+ cells were cocultured with PMVECs for 7 days in the presence of optimal concentrations of granulocyte / macrophage colony stimulating factor ( GM CSF ) + interleukin ( IL ) 3 + IL 6 + stem cell factor ( SCF ) + Flt 3 ligand . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
In this study we have investigated the effect of the early acting haemopoietic cytokines flt 3 ligand ( FL ) , IL 3 and GM CSF on the SCF dependent differentiation of mast cells from cord blood mononuclear cells . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Different culture conditions were generated by adding G CSF , granulocyte macrophage colony stimulating factor ( GM CSF ) , Flt 3 ligand ( Flt 3 ) , megakaryocyte growth and development factor ( Peg rHuMGDF ) , GM CSF / erythropoietin ( EPO ) hybrid protein ( MEN 11303 ) , and interleukin 15 ( IL 15 ) to the basic stem cell factor ( SCF ) + interleukin 3 ( IL 3 ) + EPO combination . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Long term culture of human CD 34 ( + ) progenitors with FLT 3 ligand , thrombopoietin , and stem cell factor induces extensive amplification of a CD 34 ( ) CD 14 ( ) and a CD 34 ( ) CD 14 ( + ) dendritic cell precursor . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
This features the use of a serum free medium supplemented with the cytokines flt 3 ligand , IL 3 , stem cell factor ( c kit ligand ) and IL 2 , together with IL 7 or oncostatin M ( OM ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Other cytokines that could be detected were IL 6 , M CSF , SCF , flt 3 ligand . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
The assay is done in contact with murine AFT 024 fetal liver stromal cells and with Flt 3 Ligand , stem cell factor , and interleukin 7 . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
To characterize the fate of early hematopoietic progenitor cells during ex vivo expansion in suspension culture , human CD 34 ( + ) enriched BPC were cultured in serum free medium in the presence of FLT 3 ligand ( FL ) , stem cell factor ( SCF ) and interleukin 3 ( IL 3 ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
RESULTS : Under our optimized conditions of mouse BM incubation ( stem cell factor plus interleukin 11 , either with or without macrophage inflammatory protein 1alpha or Flt 3 ligand ) , both the short term and the mid term repopulating ability of the ex vivo expanded samples were significantly improved when compared with fresh samples . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Target cells were exposed daily to vector containing supernatants and a combination of interleukin 3 ( IL 3 ) , interleukin 6 ( IL 6 ) , stem cell factor ( SCF ) and Flt 3 ligand ( FL ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
MPB purified CD34+ Thy 1+ cells were labeled with CFSE dye and cultured for 112 hours in serum deprived medium in the presence of the cytokine combinations of thrombopoietin ( TPO ) , flt 3 ligand ( FL ) , and c kit ligand ( KL ) , or TPO , FL , and interleukin 6 ( IL 6 ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Comparing several different combinations of cytokines added to 90 hour cultures of MPB CD 34 cells , thrombopoietin ( TPO ) , flt 3 ligand ( FL ) , and c kit ligand ( KL ) gave the best result , with the lowest percentage of apoptotic cells and a mean 1 . 2 fold increase in the number of CD34+ Thy 1+ cells . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
We examined the effect of recombinant human interleukin ( IL ) 11 alone or in combination with various colony stimulating factors ( CSFs ) , including IL 3 , granulocyte / macrophage ( GM ) CSF , granulocyte ( G ) CSF , stem cell factor ( SCF ) , flt 3 ligand ( FL ) , and thrombopoietin ( TPO ) , on colony formation by leukemic progenitor cells ( L CFU ) obtained from 33 patients with acute myelogenous leukemia ( AML ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Therefore , we have attempted to maintain human cord blood or bone marrow CD 34 ( + ) cells ex vivo in serum free cultures containing stem cell factor ( SCF ) and FLT 3 ligand ( FL ) alone or together with a new recombinant molecule of soluble IL 6 receptor fused to IL 6 ( IL6RIL6 chimera ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
In short term cultures containing erythropoietin ( Epo ) , interleukin ( IL ) 1 , IL 3 , and IL 6 , or granulocyte ( G ) and granulocyte macrophage colony stimulating factor ( GM CSF ) together with stem cell factor ( SCF ) or Flt 3 ligand ( FL ) , expansion of CFUs was six to eightfold at week 1 . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
The production of haemopoiesis inhibitor by patient cells and the influence of high concentrations of stem cell factor ( SCF ) and Flt 3 ligand ( FL ) on cell survival and ex vivo expansion potential were also studied . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Monocytic differentiation of CD 34 ( + ) cells supported by FLT 3 ligand and interleukin 3 ( IL 3 ) was correlated to high levels of hypophosphorylated pRb , whereas neutrophilic differentiation , supported by granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) , was correlated to low levels . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
After single cell sorting , the CD 34 ( + ) / CD38 ( ) cells derived from FLV were exposed to flt 3 ligand , thrombopoietin , stem cell factor ( SCF ) , or medium containing a cytokine cocktail ( with SCF , interleukin 3 , interleukin 6 , granulocyte macrophage colony stimulating factor , and erythropoietin ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
CD34+ cells isolated from human CB were cultured in a stroma free liquid culture system with thrombopoietin ( TPO ) , flt 3 ligand ( FL ) , stem cell factor ( SCF ) , and / or granulocyte colony stimulating factor ( G CSF ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
The reduction of in vitro radiation induced Fas related apoptosis in CD34+ progenitor cells by SCF , FLT 3 ligand , TPO , and IL 3 in combination resulted in CD34+ cell proliferation and differentiation . ^^^ The early effects of exogenous cytokines at the hematopoietic stem and progenitor cell ( HSPC ) level following irradiation are still largely unknown , especially those of survival factors such as stem cell factor ( SCF ) and Flt 3 ligand ( FL ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Based on our earlier study of the conditions required for ex vivo expansion of Lin ( ) CD 34 ( + ) CD 38 ( ) cells and SRC , CD 34 ( + ) enriched lineage depleted umbilical cord blood cells were cultured for 2 to 6 days on fibronectin fragment in MGIN ( MSCV EGFP Neo ) retroviral supernatant ( containing 1 . 5 % fetal bovine serum ) and IL 6 , SCF , Flt 3 ligand , and G CSF . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Medium for the ex vivo cultures contained either serum and six recombinant human hematopoietic growth factors ( GFs ) , including Flt 3 ligand ( FL ) , Kit ligand ( KL = stem cell factor ) , thrombopoietin ( Tpo ) , interleukin 3 ( IL 3 ) , granulocyte colony stimulating factor ( G CSF ) , and interleukin 6 ( IL 6 ) , or a serum free medium containing only FL , KL , and Tpo . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
The transduction protocol used the CH 296 recombinant human fibronectin fragment and relatively high concentrations of the flt 3 ligand and stem cell factor . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Compared with interleukin 3 ( IL 3 ) , IL 6 , and leukemia inhibitory factor ( LIF ) , the combination of thrombopoietin ( TPO ) , Flt 3 ligand ( FL ) , and Kit ligand ( KL ) effected a 73 fold increase in NGFR expression among CD34+ cells ( to 14 % ) and a 14 fold increase in NGFR expression among total cells ( to 10 % ) after LTC . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
In the presence of early acting cytokines interleukin 1beta ( IL 1beta ) , IL 3 , IL 6 , stem cell factor ( SCF ) , flt 3 ligand with and without thrombopoietin ( Tpo ) , we compared the expansion capacity of CD34+ enriched cells from CB , NB and bone marrow ( BM ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NALM 29 cells responded significantly to the proliferative stimuli of FLT 3 ligand and IL 7 , but not to GM CSF , IL 3 , IL 6 , PIXY 321 or SCF . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Immunoselected CD34+ cells from peripheral blood stems cells ( PBSCs ) were cultured in media with or without serum , supplemented by IL 3 , IL 6 , IL 11 , SCF , TPO , Flt 3 ligand , and MIP 1alpha . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Dendritic cells generated from CD34+ progenitor cells with flt 3 ligand , c kit ligand , GM CSF , IL 4 , and TNF alpha are functional antigen presenting cells resembling mature monocyte derived dendritic cells . ^^^ The combination of cytokines flt 3 ligand + c kit ligand + granulocyte macrophage colony stimulating factor ( GM CSF ) + interleukin 4 ( IL 4 ) + tumor necrosis factor alpha ( TNF alpha ) was used to maximize the output of mature DCs in the culture of CD34+ cells while minimizing the production of monocytes . ^^^ Differentiation was marked by a time dependent increase in allostimulatory function , which , at its peak , was more potent than in cultures of DCs generated from monocytes with GM CSF + IL 4 , but was comparable on a cell to cell basis to that of mature monocytes cultured in flt 3 ligand + c kit ligand + GM CSF + IL 4 + TNF alpha . ^^^ Altogether , these results suggest that DCs generated from CD34+ cells of patients with breast cancer with flt 3 ligand , c kit ligand , GM CSF , IL 4 , and TNF alpha are competent antigen presenting cells , particularly for CD8+ cytotoxic T lymphocytes , and resemble mature monocyte derived DCs in the assays described here . . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
The in vitro radiation sensitivity of CFU Meg isolated from human placental and umbilical cord blood was evaluated in plasma clot cultures stimulated by recombinant human cytokines , including thrombopoietin , the FLT 3 ligand ( FLT3LG ) , interleukin 3 , interleukin 11 and stem cell factor . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Culture of bone marrow precursor cells with cytokines , including granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 4 ( IL 4 ) and the tyrosine kinase receptor binding proteins Flt 3 ligand ( Flt 3L ) and stem cell factor ( SCF ) , has previously been shown , in both mouse and human , to result in the generation of large numbers of dendritic cells . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
To investigate the clinically applicable conditions that support substantial expansion of both primitive and more mature hematopoietic cells of umbilical cord blood ( UCB ) for transplantation in adults , enriched CD34+ cells from 8 fresh UCB samples and 4 expanded UCB products were cultured in defined serum free medium ( QBSF 60 ) in the presence of a cytokine combination of SCF , Flt 3 ligand ( FL ) , thrombopoietin ( TPO ) , IL 3 for up to 2 weeks . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Cord blood CD34+ cells were transduced to high levels over 24 48 hr in the presence of Flt 3 ligand , stem cell factor , interleukin 3 , and interleukin 6 . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
A large number of cells with the morphological , phenotypical , and functional characteristics of DCs was generated from both c kit ( low ) and c kit ( low ) populations when cultured with a combination of cytokines ( GM CSF , tumor necrosis factor a [ TNF a ] , interleukin 7 [ IL 7 ] , IL 3 , stem cell factor [ SCF ] , and flt 3 ligand ) ; the cytokine combination studies revealed that SCF and IL 3 in addition to GM CSF and TNF a are essential for DCs to be generated from these primitive populations . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Bone marrow and umbilical cord blood CD34+ cells were cultured in serum free medium under survival conditions with thrombopoietin ( Tpo ) alone , or under proliferating conditions with Tpo , c Kit ligand ( KL ) , and Flt 3 ligand ( FL ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
We demonstrate here that LC development from human CD34+ hemopoietic progenitor cells in response to TGF beta 1 costimulation ( basic cytokine combination GM CSF plus TNF alpha , stem cell factor , and Flt 3 ligand ) is associated with pronounced cell cluster formation of developing LC precursor cells . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
A combination of stem cell factor , Flt 3 ligand and IL 7 was also able to support the proliferation and differentiation of the progenitors in a suspension culture . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
The addition of other growth factors such as SCF and Flt 3 ligand can dramatically enhance DC recovery . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Twelve cases of human AML were tested for ability to be differentiated toward a professional APC phenotype in short term culture with addition of GM CSF and the following recombinant proteins : TNFalpha , IL 4 , CD 40 ligand , Flt 3 ligand and SCF . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Also in CD34+ precursors , cultured in the presence of flt 3 ligand , c Kit ligand , GM CSF , IL 4 and TNF alpha , addition of anti CD 33 mAb prevented the recovery of mature dendritic cells . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
The highest yield of CD1a ( + ) / CD14 ( ) cells could be obtained with stem cell factor , transforming growth factor beta , tumor necrosis factor alpha , GM CSF , and FLT 3 ligand . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : CD 34 ( + ) PBPC were cultured in serum free medium comparing different ( n = 32 ) combinations of stem cell factor ( S ) , interleukin 1 ( 1 ) , interleukin 3 ( IL 3 ) ( 3 ) , interleukin 6 ( 6 ) , erythropoietin ( E ) , granulocyte colony stimulating factor ( G ) , granulate macrophage colony stimulating factor ( GM ) , daniplestim ( D , a novel IL 3 receptor agonist ) , and Flt 3 ligand ( FL ) under various culture conditions . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
In the presence of IL 3 , IL 6 , SCF , or leukemia inhibitory factor ( LIF ) and Flt 3 ligand , hMSC facilitated expansion and retroviral transduction of human peripheral blood mobilized CD34+ cells . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Synergistic combinations of cytokines including TPO , interleukin ( IL ) 1 , IL 3 , IL 11 , stem cell factor , and FLT 3 ligand induce the ex vivo expansion of colony forming unit MK progenitors and MKs from cytokine mobilized peripheral blood cells , bone marrow , and cord blood CD34+ cells . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
The number of clonogenic megakaryocyte progenitors ( colony forming units megakaryocyte ; CFU Mk ) with high ( > 20 cells / colony ) and low proliferative capacity ( 5 20 cells / colony ) and the number of megakaryocytic ( CD61+ ) cells were significantly increased by including interleukin 3 ( IL 3 ) or IL 3 + IL 6 + IL 11 + Flt 3 ligand to cultures containing megakaryocyte growth and development factor ( MGDF ) plus stem cell factor ( SCF ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
In this paper the effects of flt 3 ligand ( FL ) , stem cell factor ( SCF ) , interleukin ( IL ) 3 , IL 6 , granulocyte colony stimulating factor ( G CSF ) and granulocyte monocyte colony stimulating factor ( GM CSF ) on long term ex vivo expansion and differentiation of cord blood hematopoietic cells were investigated . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
To delineate the mechanism by which NK cells begin to express different patterns of Ly 49 and CD94 / NKG2 molecules , we developed a clonal assay in which NK1 . 1 ( ) , IL 2 / IL 15 receptor beta+ NK precursors generated by culture of multipotential Lin ( ) , c kit+ progenitors in IL 7 , stem cell factor and flt 3 ligand are induced to differentiate into NK1 . 1+ , Ly49+ NK cells . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Furthermore , we have ruled out the contribution of 6 known early acting cytokines , including interleukin 3 , interleukin 6 , granulocyte macrophage colony stimulating factor , stem cell factor , flt 3 ligand , and thrombopoietin , to this SCEPF activity . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Through such studies , c kit ligand ( KL ) , flt 3 ligand ( FL ) , thrombopoietin , and IL 11 have emerged as likely positive regulators of HSC self renewal . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
New cytokine / chemokine mobilization regimens are under investigation such as combined rHuG CSF and rHu thrombopoietin , rHuG CSF and interleukin 3 , rHuG CSF and rHu stem cell factor , rHuG CSF and Flt 3 ligand , human macrophage inflammatory protein , interleukin 1 , and interleukin 8 . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
In addition , TPO directly and potently synergizes with other early acting cytokines ( kit ligand , flt 3 ligand and interleukin 3 ) to promote multilineage growth of the same progenitor cell population . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
The expansion conditions were : 1 . 5 10 10 ( 3 ) cells / ml in serum free medium containing stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , IL 6 , granulocyte stimulating factor ( G CSF ) , erythropoietin ( Epo ) + / Flt 3 ligand ( Flt 3 L ) for 10 days . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Three early acting ( stem cell factor ( SCF ) , Flt 3 ligand ( Flt 3 ) and fetal antigen 1 ( FA 1 ) ) and three lineage specific growth factors ( EPO , G CSF and thrombopoietin ( Tpo ) ) were analyzed by ELISA in 16 patients with multiple myeloma ( MM ) and 16 patients with non Hodgkin ' s lymphoma ( NHL ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
In physical or biological characteristics , it exhibited a unique character different from IL 3 , IL 6 , GM CSF , SCF or FLT 3 ligand those are known to have hematopoietic stem cell proliferation stimulating activity . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Cells were prestimulated with flt 3 ligand , stem cell factor , and interleukin 3 and transduced on fibronectin . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
PMVEC coculture supplemented with GM CSF + IL 3 + IL 6 + SCF + Flt 3 ligand induced > 93 % of the CD 34 ( + ) CD 38 ( ) population to enter the G ( 1 ) or G ( 2 ) / S / M phase while increasing this population from 1 . 4 % on day 0 to 6 . 5 % of the total population by day 5 . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
The cytokines studied were stem cell factor ( SCF ) , interleukin ( IL ) 3 , megakaryocyte growth and development factor ( MGDF ) , granulocyte colony stimulating factor ( G CSF ) , Flt 3 ligand , IL 6 , IL 11 , and erythropoietin ( EPO ) . ^^^ The effect of Flt 3 ligand and SCF factor on CD34+ cell production was also less than additive , although the response to both cytokines was greater than single cytokines . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Using a 1 day cytokine mediated prestimulation , consisting of human interleukin ( IL ) 3 , IL 6 , stem cell factor ( SCF ) , Flt 3 ligand ( FL ) , and thrombopoietin ( TPO ) , followed by a 3 day transduction procedure , we were able to detect up to 51 % CD 34 ( + ) cells expressing MDR 1 . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Hemopoietic cells were grown in long term cultures in the presence of various combinations of early acting cytokines such as FLT 3 ligand ( FLT 3 L ) and stem cell factor ( SCF ) and the differentiating growth factors GM CSF and TNF alpha . ^^^ Stem cell factor and FLT 3 ligand are strictly required to sustain the long term expansion of primitive CD34+DR dendritic cell precursors . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Stimulation of mouse bone marrow cells with kit ligand , FLT 3 ligand , and thrombopoietin leads to efficient retrovirus mediated gene transfer to stem cells , whereas interleukin 3 and interleukin 11 reduce transduction of short and long term repopulating cells . ^^^ Large quantitative differences in reconstitution were observed after transplantation with graded numbers ( 1000 30 , 000 ) of EGFP ( + ) cells preconditioned with various combinations of Kit ligand ( KL ) , FLT 3 ligand ( FL ) , thrombopoietin ( TPO ) , interleukin 3 ( IL 3 ) , and IL 11 . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Among the cytokine combinations tested , SCF + IL 3 + sIL 6R / IL 6 + flt 3 ligand ( FL ) most effectively expanded CFU GM and CFU Mix . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
In cultures with SCF , Flt 3 ligand ( FL ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and tumor necrosis factor ( TNF ) alpha ( standard condition ) , CD 7 ( + ) CD45RA ( + ) progenitors expanded less ( 37 vs 155 fold ) but yielded 2 fold higher CD1a ( + ) DC percentages than CD 7 ( ) CD45RA ( + ) or CD 7 ( + ) CD45RA ( ) progenitors . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
CD34+ cells harvested from umbilical cord blood were kept in suspension cultures and stimulated with combinations of the cytokines stem cell factor ( SCF ) , FLT 3 ligand , interleukin 3 ( IL 3 ) , IL 6 , and IL 7 prior to transduction . ^^^ Then , prestimulation with SCF and FLT 3 ligand was compared . ^^^ Interestingly , FLT 3 ligand seemed to preserve the population of CD34+CD38 cells compared to SCF ( 16 . 56 % + / 2 . 02 versus 9 . 39 % + / 2 . 35 , p = 0 . 03 ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
CD34+ hematopoietic cells were cultured in serum free medium with megakaryocyte growth and development factor ( MGDF ) alone for supporting the survival of primitive progenitors or with MGDF , c kit ligand ( KL ) and flt 3 ligand ( FL ) for inducing proliferation of primitive progenitors . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
STUDY DESIGN AND METHODS : Cord blood CD34+ cells were cultured in Iscove ' s modified Dulbecco medium supplemented with 10 percent fetal calf serum ( FCS ) and IL 6 , SCF , megakaryocyte growth and development factor , and Flt 3 ligand . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Interleukin 3 ( IL 3 ) , stem cell factor ( SCF ) , and flt 3 ligand ( FL ) are potential candidates for expansion strategies due to their early acting and lineage unspecific hematopoietic stimulation . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Flt 3 ligand ( FL ) is a cytokine that promotes the survival , proliferation , and differentiation of hematopoietic progenitors in synergy with other growth factors , such as stem cell factor . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
CD34 ( + ) cord blood ( CB ) cells were cultured in serum free medium containing stem cell factor , Flt 3 ligand , megakaryocyte growth and development factor , and granulocyte colony stimulating factor . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
DESIGN AND METHODS : AML blasts were maintained with or without cytokines ( stem cell factor , FLT 3 ligand and interleukin 3 ) for 48 hours , and successively infected with two spin infection cycles . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
IL 11 and the Flt 3 ligand possessed T lineage promotional effects similar to IL 6 and SCF respectively . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Using a combination of FLT 3 ligand ( FL ) , Stem Cell Factor ( SCF ) , Thrombopoietin ( TPO ) and Interleukin 6 ( IL 6 ) , we cultured cord blood ( CB ) CD34+ cells for up to 12 weeks and transplanted their progeny into sublethally irradiated NOD / SCID mice . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Initial in vitro studies with rhesus CD34+ cells indicated that after 4 days of stimulatory culture in stem cell factor ( SCF ) , megakaryocyte growth and development factor ( MDGF ) , and flt 3 ligand ( FLT ) , transfer of the cells to SCF alone on retronectin ( FN ) support resulted in decreased active cycling and a halt to proliferation , without a loss of viability or induction of apoptosis . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Whereas Flt 3 ligand ( FL ) , stem cell factor ( SCF ) , and thrombopoietin ( TPO ) are considered indispensable , the role of interleukin 3 ( IL 3 ) is still controversial : it has been reported either to support or abrogate the reconstituting ability of stem cells . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
OBJECTIVE : We evaluated the frequency of hematopoietic progenitor cells ( HPC ) in CD 34 ( + ) CLA ( + ) ( cutaneous lymphocyte associated antigen ) and CD 34 ( + ) CLA ( ) cord blood cells , and followed cellular growth and HPC production during cultures in Flt 3 ligand , thrombopoietin , and stem cell factor ( FTS ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
METHODS : CD34+ cells were transduced with lentivectors encoding for GFP before or after 21 days of culture with FLT 3 ligand , thrombopoietin and stem cell factor and induction into DC with GM CSF+IL 4 ( G 4 ) or G4+TNF ( GT 4 ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
To assess the hematopoietic defect involved in PNH , CD34+ CD59+ ( normal phenotype hematopoietic stem / progenitor ) and CD34+ CD 59 ( PNH phenotype ) cells from PNH patients ( n = 16 ) and CD34+ CD59+ cells from healthy volunteers ( n = 10 ) were sorted as single cells into 96 well flat bottom culture plates containing culture medium supplemented with stem cell factor , interleukin ( IL ) 3 , erythropoietin , granulocyte macrophage colony stimulating factor ( GM CSF ) , G CSF , IL 6 , thrombopoietin , and Flt 3 ligand . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Human peripheral blood ( PB ) CD34+ cells ( > or = 90 % purity ) were cultured for up to 4 days in serum free culture medium supplemented with thrombopoietin ( TPO ) , stem cell factor ( SCF ) , Flt 3 ligand ( Flt 3L ) , with or without PIXY 321 ( IL 3 / GM CSF fusion protein ) and human serum . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Cell cycle activation of peripheral blood stem and progenitor cells expanded ex vivo with SCF , FLT 3 ligand , TPO , and IL 3 results in accelerated granulocyte recovery in a baboon model of autologous transplantation but G0 / G1 and S / G2 / M graft cell content does not correlate with transplantability . ^^^ We showed in a nonhuman primate model of autologous peripheral blood stem and progenitor cell transplantation that cell cycle activation of CD 34 ( + ) cells in the presence of stem cell factor + FLT 3 ligand + thrombopoietin + interleukin 3 ( six days of culture ) which induced G 1 and S / G2 / M cell amplification ( G 0 : 6 . 1 % + / 2 . 8 % ; G0 / G1 : 64 . 2 % + / 7 . 2 % ; S / G2 / M : 30 . 4 % + / 7 . 3 % respectively of expanded CD 34 ( + ) cells on average ) resulted in the acceleration of short term granulocyte recovery . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
We investigated the maintenance of primitive SCID repopulating cells ( SRC ) and long term culture initiating cells ( LTCIC ) in PBSC expanded with early acting cytokines , thrombopoietin ( TPO ) , stem cell factor ( SCF ) and FlT 3 ligand ( FL ) with or without interleukin 3 ( IL 3 ) and IL 6 in short term ( 6 d ) stroma free serum free cultures . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
The combination of thrombopoietin , Flt 3 ligand , and stem cell factor upregulated survivin expression in CD 34 ( + ) cells within 24 hours ; survivin expression was cell cycle related and highest during G2 / M , whereas growth factor withdrawal resulted in decreased survivin expression . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
CD34+ cells were cultured in 10 VIVO 10 supplemented with thrombopoietin , stem cell factor , and Flt 3 ligand . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
We examined the functional differences between G ( 0 ) and G ( 1 ) cord blood CD34+ cells for up to 24 weeks in serum free suspension culture , containing Flt 3 ligand , thrombopoietin and stem cell factor . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Among various combinations , the combination of c kit ligand , flt 3 ligand , c mpl ligand ( TPO ) , and interleukin ( IL ) 4 most potently generated the number of CD1a+CD14 DCs in cultures containing granulocyte macrophage colony stimulating factor ( GM CSF ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^ These findings demonstrated that the combination of c kit ligand plus flt 3 ligand plus TPO with GM CSF plus TNF alpha , followed by IL 4 , is useful for ex vivo generation of human DCs from mobilized CD34+ peripheral blood progenitors . . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
METHODS : G CSF mobilized peripheral CD 34 ( + ) cells were cultured for 4 days with stem cell factor , flt 3 ligand , and thrombopoietin . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Apart from granulocyte macrophage CSF ( GM CSF ) , there have as yet been no reports of lithium salts inducing early activating factors for the megakaryocytic lineage , such as IL 3 , IL 11 , stem cell factor and flt 3 ligand , or maturation factors , such as thrombopoietin ( TPO ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
It could be increased in 5 7 days when the cells were cultured in vitro , especially in the presence of stem cell factor , interleukin 3 ( IL 3 ) , IL 6 and Flt 3 Ligand together ; the positive rate reached 48 % . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
STUDY DESIGN AND METHODS : Enriched CB CD34+ cells were cultured in four media ( Iscove ' s modified Dulbecco ' s medium with FCS , Gibco ; 10 Vivo 10 , BioWhittaker ; QBSF 60 , Quality Biological ; and StemSpan SFEM , Stem Cell Technologies ) with four cytokine combinations ( thrombopoietin [ TPO ] , SCF , Flt 3 ligand [ FL ] with and without G CSF , and / or IL 6 ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
On the other hand , SDF 1 significantly increased the viability of CD 34 ( + ) T cell precursors modulating the expression of BCL 2 and BAX genes , and stimulated the proliferation of CD 34 ( + ) thymic precursor cells , particularly in synergy with interleukin 7 ( IL 7 ) , but not with other cytokines , such as stem cell factor or flt 3 ligand . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
The growth kinetics of two populations of muscle stem cells were characterized in culture medium supplemented with epidermal growth factor ( EGF ) , fibroblast growth factor 2 ( FGF 2 ) , insulin like growth factor 1 ( IGF 1 ) , FLT 3 ligand , hepatocyte growth factor , or stem cell factor ( SCF ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
In the present study , several cytokines , flt 3 ligand , stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , IL 6 , IL 11 , leukemia inhibitory factor , G CSF , and erythropoietin , which are known to affect megakaryocytopoiesis , have been evaluated to elucidate their effects on the TPO induced apoptosis . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
The quantification analyses have revealed the increase of the amounts of IL 6 and flt 3 ligand in their serum , but not significant increase of the amount of SCF . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Stem Cell Factor ( SCF ) + IL 6 and FLT 3 ligand , which have no effect on colony formation by themselves , were used to enhance the effects of IL 3 and GM CSF , respectively . ^^^ Stem Cell Factor ( SCF ) + IL 6 and FLT 3 ligand , which have no effect on colony formation by themselves , were used to enhance the effects of IL 3 and GM CSF , respectively . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Ex vivo expansion of stem and progenitor cells in co culture of mobilized peripheral blood CD34+ cells on human endothelium transfected with adenovectors expressing thrombopoietin , c kit ligand , and Flt 3 ligand . ^^^ Immortalized human bone marrow endothelial cells ( BMEC ) transfected with an expression vector carrying cDNA encoding the human telomerase reverse transcriptase ( hTERT ) and human umbilical vein endothelial cells ( HUVEC ) were transfected with combinations of adenovectors expressing murine c kit ligand ( KL ) , human thrombopoietin ( TPO ) , human Flt 3 ligand ( FL ) , and human granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Human CD34+ cells from CB or PBSC were cultured in serum free medium , with or without hematopoietic growth factors ( FKT : FLT 3 ligand [ FL ] , KIT ligand [ KL ] , and thrombopoietin [ TPO ] ) , and with or without soluble Fas ligand ( sFasL ) or agonistic anti Fas antibody . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
In this study , we investigated the effects of the cytokines flt 3 ligand , stem cell factor , and thrombopoietin on expansion of CD34+ and CD34+ / CD38 cells . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Here , we demonstrate the selective expansion of CD34+CD38 cells from purified CD34+ cells upon stimulation with Flt 3 ligand , stem cell factor and thrombopoietin . ^^^ We conclude that the combination Flt 3 ligand ( FL ) , stem cell factor and thrombopoietin ( TPO ) induced strong ex vivo proliferation of CD34+CD38 cells and that the absolute number of expanded cells with stem cell activity increased substantially in this population . . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Expansion of haemopoietic stem cells from placental blood has been obtained with a combination of flt 3 ligand ( FL ) , thrombopoietin ( TPO ) , kit ligand ( KL ) with or without interleukin 6 ( IL 6 ) in serum replete medium . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
In contrast , rhSCGF alpha suppressed stem cell factor ( SCF ) stimulated erythroid bursts , indicating some competitive interaction between SCGF and SCF . rhSCGF alpha was synergistic with interleukin 3 and the flt 3 ligand to enhance GM colony growth , but not synergistic with those inducing ex vivo expansion of GM progenitor cells . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
CIP 29 mRNA expression is cytokine regulated in hematopoietic cells , being up regulated by Epo in UT7 / Epo cells , and by thrombopoietin ( Tpo ) , FLT 3 ligand ( FL ) and stem cell factor ( SCF ) in primary human CD 34 ( + ) cells . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Cell surface immunophenotyping of spleen derived fibroblasts revealed a low and consistent expression of IL 15 , Flt 3 ligand , and c kit ligand . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Strain dependent variation existed among C57BL / 6 ( B 6 ) , DBA / 2 , and BXD recombinant inbred mice in the responsiveness of primitive progenitor cells to the early acting cytokines kit ligand , flt 3 ligand , and thrombopoietin . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
In the current study , we demonstrated that it is possible to harvest about 28 to 80 fold more veto cells on culturing of purified CD 34 ( + ) cells for 7 to 12 days with an early acting cytokine mixture including Flt 3 ligand , stem cell factor , and thrombopoietin . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
The LC cells were cultured in serum free culture medium supplemented with a combination of early acting cytokines including thrombopoietin ( TPO ) , flt 3 ligand ( FL ) , and stem cell factor ( SCF ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Lin ( ) Sca 1 ( + ) HPC cultured 9 days in 100 ng / mL stem cell factor ( SCF ) , 20 ng / mL interleukin 3 ( IL 3 ) , 50 ng / mL monocyte colony stimulating factor ( M CSF ) , 5 ng / mL granulocyte monocyte colony stimulating factor ( GM CSF ) , and 25 ng / mL FLT 3 ligand ( FLT 3 L ) proliferate 387 fold and differentiate into DC precursors . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
MATERIAL AND METHODS : To perform our experiments we used a stroma free cell culture system in which the combination of FLT 3 ligand ( FL ) , stem cell factor ( SCF ) and thrombopoietin ( TPO ) induces massive expansion and proliferation of cord blood HP . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Proliferative response of blast clonogenic cells to various hematopoietic growth factors ( HGF ) , including stem cell factor ( SCF ) and flt 3 ligand ( FL ) was investigated in 100 patients with acute myeloid leukemia ( AML ) and chronic myelogenous leukemia ( CML ) in myeloid crisis ( MC ) . ^^^ Differential response to stem cell factor and Flt 3 ligand by the FAB subtype in acute myeloid leukemia clonogenic cells . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
In the expansion group , CD34+ cells were cultured for 6 d with a combination of early acting cytokines ( Flt 3 ligand , stem cell factor , thrombopoietin and interleukin 3 ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Recipient mice were treated for 1 or 7 days with growth factors ( stem cell factor [ SCF ] plus interleukin 11 [ IL 11 ] , SCF plus Flt 3 ligand [ FL ] , or granulocyte colony stimulating factor [ G CSF ] ) prior to low dose TBI ( 4 Gy ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Combinations of thrombopoietin and cytokines that act on megakaryocyte development ( stem cell factor , IL 3 , IL 6 , IL 11 , flt 3 ligand ( now known as FLT3LG ) , erythropoietin , GM CSF and G CSF were evaluated for their ability to enhance clonal growth in vitro of 10 irradiated CD 34 ( + ) megakaryocytic progenitor cells ( CFU megakaryocytes ) purified from normal human peripheral blood . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
METHOD : The CD ( 34 ) ( + ) CD ( 59 ) ( + ) and CD ( 34 ) ( + ) CD ( 59 ) ( ) cells from PNH patients and CD ( 34 ) ( + ) CD ( 59 ) ( + ) cells from normal volunteers were sorted as each single cell into a well of 96 well culture plates containing culture medium supplemented with SCF , IL 3 , Epo , GM CSF , G CSF , IL 6 , Tpo and Flt 3 ligand . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
METHODS : CD34+ cell preparations purified from CB or PBSC were cultured in serum free medium containing FKT : FLT 3 ligand ( FL ) , KIT ligand ( KL ) , and thrombopoietin ( TPO ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Cord blood CD34+ cells were incubated with supernatants containing retrovirus for 48 h with cytokines such as IL 3 , IL 6 , SCF , Flt 3 ligand ( FL ) , bFGF and IGF 1 . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
METHODS : Human mobilized peripheral blood ( MPB ) CD 34 ( + ) cells were cultured with the cytokines thrombopoietin mimetic peptide ( mTPO ) , flt 3 ligand ( FL ) , and c kit ligand ( KL ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Residual CD34+ cells were successfully expanded in serum free medium in the presence of antiapoptotic cytokine combination ( stem cell factor + FLT 3 ligand + thrombopoietin + interleukin 3 , 50 ng / mL each , i . e . , 4F ) : KCD34+ = x2 . 8 and x13 . 7 ( n = 2 ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
The first consisted of a 7 day culture in the presence of stem cell factor ( SCF ) , Flt 3 ligand , thrombopoietin ( TPO ) , interleukin 3 ( IL 3 ) , and IL 6 , which induced preferentially the expansion of immature hematopoietic cells [ 3 . 1 + / 1 . 4 , 10 . 0 + / 5 . 1 , 2 . 2 + / 1 . 9 , and 1 . 0 + / 0 . 3 fold expansion for mononuclear cells ( MNC ) , colony forming units granulocyte macrophage ( CFU GM ) , burst forming units erythroid ( BFU E ) , and long term culture initiating cells ( LTC IC ) respectively ] . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Apoptosis may be significantly reduced with a combination of 4 cytokines ( 4F ) : Stem Cell Factor ( SCF ) , FLT 3 Ligand ( FL ) , thrombopoietin ( TPO ) , and interleukin 3 ( IL 3 ) , each at 50 ng 10 mL ( 1 ) ( 15 % survival versus < 3 % untreated cells , 24 h post irradiation at 2 . 5 Gy ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Sorted NBM CD 34 ( + ) CD 133 ( + ) and CD 34 ( + ) CD 133 ( ) subsets were evaluated for Mk clonogenic capacity before and after in vitro proliferation in serum free liquid culture containing kit ligand , Flt 3 ligand , thrombopoietin , interleukin 3 , and interleukin 6 . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Up regulation of survivin by thrombopoietin ( Tpo ) , Flt 3 ligand ( FL ) , and stem cell factor ( SCF ) occurred in underphosphorylated retinoblastoma protein ( Rb ) ( positive ) , Ki 67 ( negative ) , and cyclin D ( negative ) CD 34 ( + ) cells . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Recent studies have identified thrombopoietin ( TPO ) , flt 3 ligand ( FL ) , Steel factor ( SF ) , and interleukin 11 ( IL 11 ) as cytokines able to stimulate amplification of the most primitive murine hematopoietic cells in vitro . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Consistent with the in vivo repopulating deficiency , when Sca 1 ( + ) c kit ( + ) lin ( ) ( KLS ) cells were isolated and stimulated with growth factors in vitro , up to a 13 fold reduced expansion of total nucleated cells was observed in response to cocktails containing interleukin 3 ( IL 3 ) , IL 6 , stem cell factor ( SCF ) , Flt 3 ligand , and thrombopoietin . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
During myeloid / monocytic differentiation induced by incubation with stem cell factor , thrombopoietin , and flt 3 ligand , partially differentiated HSCs emerge , which readily take up these pathogens and also latex beads by macropinocytosis . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
This study investigated early ex vivo expansion kinetics of UCB AC133+ cells ( 2 4 10 10 ( 4 ) / ml ) , mononuclear cells ( MNC , 1 2 10 10 ( 6 ) / ml ) and AC133negative cells ( AC 133 ( neg ) , 2 4 10 10 ( 4 ) / ml ) in stroma free 8 d liquid culture ( fetal bovine serum supplemented Iscove ' s modified Dulbecco ' s medium ( IMDM ) with either ' K36EG ' [ c Kit ligand , interleukin 3 ( IL 3 ) , IL 6 , erythropoietin , granulocyte colony stimulating factor ] or ' TPOFL ' ( thrombopoietin , Flt 3 ligand ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Pure population of CD 34 ( + ) cells was incubated in a serum free medium supplemented with thrombopoietin , stem cell factor and Flt 3 ligand for 48 h and then transfected with plasmid DNA carrying the enhanced version of green fluorescent protein ( EGFP ) as a reporter gene . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
In contrast , we previously reported that Survivin is expressed throughout the cell cycle in normal CD 34 ( + ) hematopoietic stem and progenitor cells stimulated by the combination of Thrombopoietin ( Tpo ) , Stem Cell Factor ( SCF ) and Flt 3 ligand ( FL ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Interestingly , after 72 hours in serum free culture with or without Steel factor ( SF ) , Flt 3 ligand ( FL ) , and interleukin 3 ( IL 3 ) , most G ( 0 ) AML cells entered active cell cycle ( percentage of AML cells remaining in G ( 0 ) at 72 hours , 1 . 2 % to 37 % , and 0 % to 7 . 6 % in cultures without and with growth factors [ GFs ] , respectively ) while G ( 0 ) cells from normal lineage depleted bone marrow remained quiescent in the absence of GF . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
This work was aimed at evaluating the in vivo survival effect of combinations of early acting cytokines whose antiapoptotic activity has been demonstrated in vitro : stem cell factor ( SCF [ S ] ) , FMS like tyrosine kinase 3 ligand ( FLT 3 ligand [ F ] ) , thrombopoietin ( TPO [ T ] ) , interleukin 3 ( IL 3 [ 3 ] ) , and stromal derived factor 1 ( SDF 1 ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
OBJECTIVE : To analyze the expression of the CD ( 40 ) and its ligand ( CD ( 40 ) L ) on bone marrow stromal cells ( BMSC ) , and investigate interleukin 3 ( IL 3 ) , IL 6 , Flt 3 ligand ( FL ) and stem cell factor ( SCF ) production of BMSC after stimulated with CD ( 40 ) agonistic monoclonal antibody ( 5C11 ) and its role in the regulation of hematopoiesis . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
E2A expression in vitro was also assessed in cultures supplemented with IL 7 + / recombinant murine SCF ( rmSCF ) , insulin like growth factor 1 ( rhIGF 1 ) , or Flt 3 ligand ( rhFlt 3 L ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
The cytokines included the recombinant human megakaryocyte growth and development ( 100 ng / ml ) , interleukin 3 ( 10 ng / ml ) , stem cell factor ( 100 ng / ml ) , flt 3 ligand ( 50 ng / ml ) and interleukin 11 ( 200 ng / ml ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
We investigated the role of three parameters : A ) the culture medium ( serum free versus serum dependent ) ; B ) the initial cell population ( Ficoll separated mononucleated cells versus CD 34 ( + ) selected cells ) , and C ) the low concentration of recombinant cytokines , flt 3 ligand , and thrombopoietin in association with a basic cocktail of stem cell factor , interleukin ( IL ) 6 , IL 3 , GM CSF , and erythropoietin . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Cells were cultured with stem cell factor ( SCF ) , thrombopoietin ( TPO ) , Flt 3 ligand ( Flt 3 ) , and G CSF . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
We replaced granulocyte macrophage colony stimulating factor with macrophage colony stimulating factor from a serum free culture containing granulocyte macrophage colony stimulating factor , stem cell factor , Flt 3 ligand , tumor necrosis factor alpha , and transforming growth factor beta 1 . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
The highest proliferation was usually observed in the presence of Flt 3 Ligand ( Flt 3 L ) when single cytokines were examined , and these responses could be further increased especially by combining Flt 3 L with interleukin 3 ( IL 3 ) , IL 7 or stem cell factor ( SCF ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Cytokines assumed to have a stimulatory effect on HPC stem cell factor ( SCF ) , fetal liver tyrosine kinase 3 ligand ( flt 3 ligand ) , thrombopoietin ( TPO ) , stromal cell derived factor 1 ( SDF 1 ) , oncostatin M ( OSM ) , a suppressive effect on HPC proliferation macrophage inflammatory protein 1alpha ( MIP 1alpha ) , transforming growth factor beta 1 ( TGFbeta 1 ) , tumour necrosis factor alpha ( TNFalpha ) and to be involved in migration of HPC ( SCF , flt 3 ligand , MIP1alpha , SDF 1 ) were examined at the level of mRNA expression by means of real time RT PCR . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
INTRODUCTION : We compared the effects of the early acting growth factors ( GF ) , Flt 3 ligand ( FL ) , c Kit ligand ( KL ) , and leukemia inhibitory factor ( LIF ) , and the late acting GF , granulocyte colony stimulating factor ( G CSF ) and megakaryocyte growth and development factor ( MGDF ) , added alone in human long term marrow culture ( LTMC ) . ^^^ Proliferation of human progenitor cells in a long term culture system is more efficiently sustained by the addition of Flt 3 ligand or megakaryocyte growth and development factor than by Kit ligand . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
After 1 week long in vivo treatment with various combinations of interleukin ( IL ) 15 , flt 3 ligand , stem cell factor , IL 2 , IL 12 , and megakaryocyte growth and differentiation factor , CD56+CD3 cells were detected in bone marrow ( BM ) , spleen , and peripheral blood ( PB ) , comprising 5 % to 15 % of human CD45+ cells . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
When cultivated in flt 3 ligand , stem cell factor and interleukin 7 ( IL 7 ) in a stroma free culture system MLL ENL transduced as well as control cells showed a wave of B lymphopoiesis . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
METHODS : Adult normal bone marrow ( BM ) and mobilized peripheral blood ( MPB ) CD 34 ( + ) cells were cultured in a stroma free liquid culture in the presence of Flt 3 ligand ( FL ) , thrombopoietin ( TPO ) , stem cell factor ( SCF ) , interleukin 6 ( IL 6 ) , or interleukin 3 ( IL 3 ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
CD 34 progenitor cells were first amplified in vitro with stem cell factor ( SCF ) , Flt 3 ligand ( FL ) , thrombopoietin and IL 6 / soluble IL 6 receptor fusion protein , and cells were then induced to differentiate into DC with IL 4 and GM CSF . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
The cells were cultured in gas permeable Teflon coated bags in serum free medium containing the following cytokines : recombinant human interleukin 3 , recombinant human Flt 3 ligand , recombinant human stem cell factor , and recombinant human thrombopoietin . ^^^ MK precursors can be successfully expanded from CD 34 ( + ) UCB cells in a closed liquid culture system using interleukin 3 , recombinant human Flt 3 ligand , recombinant human stem cell factor , and recombinant human thrombopoietin to a level that should have a clinical impact in the transplantation setting . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
DESIGN AND METHODS : To find the best cytokine combination capable of generating large numbers of Mks , baseline CB CD34+ ( bCD34+ ) cells and CD34+ and CD 34 cells , immunoselected after 4 weeks of expansion with thrombopoietin ( TPO ) , stem cell factor ( SCF ) and Flt 3 ligand ( FL ) ( eCD34+ , eCD 34 ) , were further cultured in the presence of different cytokine combinations ( containing interleukin ( IL ) 3 , SCF , TPO and IL 6 ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
When vibrissal follicles of adult mice were cultured in the presence of stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , IL 7 , granulocyte macrophage colony stimulating factor , and Flt 3 ligand , CD45+ / lineage / c kit+ / FcepsilonRI+ cells became detectable on the outgrowing fibroblasts in 10 days and expanded progressively thereafter . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
To study the biological role of human cultured bone marrow mesenchymal stem cell ( BM MSC ) in hematopoiesis by investigation of its expression of multiple hematopoietic growth factors , RT PCR was used to analyze the expression of SCF , Flt 3 ligand , TPO , LIF , G CSF , GM CSF , IL 3 , IL 6 and IL 11 at mRNA level for human BM MSC from healthy donors and patients with leukemia and lymphoma . ^^^ The results clearly showed that the cultured BM MSC expressed mRNA of SCF , Flt 3 ligand , TPO , LIF , IL 6 and IL 11 at passages 3 up to 15 , but did not express G CSF , GM CSF and IL 3 . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
The cells were stimulated by SCF , G SCF , IL 3 and Flt 3 ligand ( FL ) added once at Day 0 . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
METHODS : The cells , plated for 7 days in serum free medium with SCF , IL 3 , G CSF , Flt 3 ligand and thrombopoietin in various combinations were assessed for the expression of CD 34 , CD 38 and CD 13 . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
METHODS : Purified human peripheral blood CD34+ cells were cultured in the presence of recombinant human IL 3 , IL 5 , rhGM CSF , SCF , and FLT 3 ligand . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
To study further the mechanisms underlying the unique patterns of hematopoietic regeneration after cyclophosphamide , mRNA levels for stem cell factor ( SCF ) , Flt 3 ligand , and macrophage inflammatory factor ( MIP ) 1 alpha cytokines were determined in bone marrow and spleen . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
IL 15 was essential and , in combination with either flt 3 ligand ( FL ) or stem cell factor ( SCF ) , was sufficient to induce the development of NKT cells . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
STUDY DESIGN AND METHODS : CD34+ cells were analyzed for the expression of CD 38 , CD 133 , and CD 117 and cultured in serum free culture medium with four cytokine combinations : SCF plus thrombopoietin plus flt 3 ligand ( STF ) , STF plus IL 3 , STF plus IL 6 , and STF plus IL 6 plus IL 3 . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
CD 34 ( + ) cells purified from ten CBs by immunomagnetic selection were cultured in serum free essential media in the presence of a combination of the several cytokines including EPO , thrombopoietin , flt 3 ligand ( FL ) , stem cell factor ( SCF ) , granulocyte colony stimulating factor , interleukin ( IL ) 3 and / or IL 11 . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Effects of a cytokine prestimulation period ( 18 hours ) with interleukin 3 , stem cell factor , Flt 3 ligand , and thrombopoietin were then analyzed in total cells , CFCs , and LTC ICs derived from mPB CD 34 ( + ) cells . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
CD34+ cells were then cultured in serum free liquid medium containing various combinations of early acting cytokines , including thrombopoietin ( TPO ) , stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) and flt 3 ligand ( FL ) , with or without bFGF , for 6 and 12 d . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
CD34+ cell derived hematopoietic precursors amplified with FLT 3 ligand , thrombopoietin and stem cell factor became , after a 6 day induction with GM CSF , IL 4 and TGF beta 1 , HLA DR+ , CD1a+ , CD 83 , CD 86 , CD 80 cells . ^^^ Both CD1a CD 14 and CD1a CD14+ cells sorted from FLT 3 ligand , thrombopoietin and stem cell factor cultures responded to TNF alpha by an increase of Langerin+ cells . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NK cells are derived from hematopoietic stem cells , for which stem cell factor or Flt 3 ligand is required in the early stage of differentiation to NK cell progenitors . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
METHODS : AC133+ cells selected from fresh cord blood ( CB ) samples were cultured in QBSF 60 serum free media in the presence of Flt 3 ligand + SCF + TPO ( FST ) , with initial addition of IL 3 for up to 2 week . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Phosphatidylinositol 3 kinase is essential for kit ligand mediated survival , whereas interleukin 3 and flt 3 ligand induce expression of antiapoptotic Bcl 2 family genes . ^^^ Cytokines such as interleukin 3 ( IL 3 ) , kit ligand ( KL ) , and flt 3 ligand ( FL ) promote survival of hematopoietic stem cells and myeloid progenitor cells . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Alteration of spontaneous proliferation by at least one mediator was detected in five of 38 patients ; whereas , altered cytokine ( Flt 3 ligand , granulocyte macrophage colony stimulating factor , stem cell factor ) dependant proliferation was observed for 10 patients . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Our results showed that the use of high concentrations of stem cell factor ( SCF ) and Flt 3 ligand ( FL ) in early CB TPO supplemented cultures was more favorable to monocytic and granulocytic cell expansion . ^^^ Preferential ex vivo expansion of megakaryocytes from human cord blood CD34+ enriched cells in the presence of thrombopoietin and limiting amounts of stem cell factor and Flt 3 ligand . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
CD34+ CD 71 CD 45 cells were cultured in defined serum free media supplemented with 300 ng each of Flt 3 ligand and stem cell factor ( SCF ) , 60 ng of interleukin ( IL ) 3 , and 20 ng each of IL 6 and G CSF . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
CD 34 ( + ) enriched cell populations from bone marrow were cultured in the presence and absence of human stroma in serum free media containing bFGF , SCF , LIF and Flt 3 ligand for several days . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Single administration of stem cell factor , FLT 3 ligand , megakaryocyte growth and development factor , and interleukin 3 in combination soon after irradiation prevents nonhuman primates from myelosuppression : long term follow up of hematopoiesis . ^^^ The present study investigated the hematopoietic response of cynomolgus monkeys to a single dose of stem cell factor , FLT 3 ligand , megakaryocyte growth and development factor , and interleukin 3 in combination ( 4F , each factor given intravenously at 50 microg / kg ) administered 2 hours after 5 Gy gamma irradiation . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
G CSF / SCF mobilized CD 34 ( + ) rhesus peripheral blood cells were cultured in the presence of SCF , Flt 3 ligand , and MGDF on Retronectin coated flasks . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
However , when bone marrow ( BM ) progenitors from IL 15Ralpha ( / ) mice were cultured with IL 7 , stem cell factor and flt 3 ligand , followed by IL 15 , they were able to differentiate into functional NK cells , indicating that IL 15Ralpha is not critical for NK cell development . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
METHODS : AC 133 ( + ) stem / progenitor cells from six full term pregnancies UCB samples were immunomagnetically selected , followed by ex vivo expansion culture in the presence of thrombopoietin ( TPO ) , c kit ligand ( KL ) , flt 3 ligand ( FL ) and IL 6 . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : HSC were enriched from steady state bone marrow by magnetic cell sorting ( lineage depletion ) and cultured in defined serum free medium containing an improved growth factor cocktail ( Flt 3 ligand , stem cell factor , interleukin 3 , interleukin 11 ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Furthermore , over 8 weeks in liquid culture stimulated by a cytokine cocktail optimized for HSPC expansion , TPOFLK ( thrombopoietin 10 ng / ml , Flt 3 ligand 50 ng / ml , c Kit ligand 20 ng / ml ) Lin cells underwent slow proliferation but maintained / expanded more primitive HSPC than CD133+ cells . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
RESULTS : Proliferation by CFU GM and BFU E was enhanced synergistically when common beta chain receptor cytokines ( IL 3 or GM CSF ) were combined with G CSF or EPO , but not with gp 130 receptor cytokines ( LIF or IL 6 ) or tyrosine kinase receptor cytokines ( SCF , HGF , Flt 3 ligand , or PDGF ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
The speed of recovery of CD11c ( + ) CD 123 ( low ) DC 1 exceeded that of CD11c ( ) CD 123 ( + ) DC 2 , and correlated with plasma levels of flt 3 ligand ( FL ) , but not with granulocyte or granulocyte macrophage colony stimulating factors and stem cell factor . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
In serum free single cell suspension culture , VIP enhanced clonal growth of CD34+ CB cells in synergy with FLT 3 ligand ( FL ) , stem cell factor ( SCF ) , and thrombopoietin ( TPO ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
In culture with stem cell factor , FLT 3 ligand , and IL 6 , a 4 hydroxytamoxifen inducible form of c Myc ( Myc / ERT ) enabled murine Lin ( ) Sca 1 ( + ) HSCs to proliferate with the surface phenotype compatible with HSCs for more than 28 days . c Myc activated by 4 hydroxytamoxifen augmented telomerase activities and increased the number of CFU Mix about 2 fold in colony assays . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
To identify putative predictors of response , patient plasma was analyzed for levels of secreted ligands of SU 5416 targets ; SCF and FLT 3 ligand . ^^^ Baseline levels of SCF in patients with stable or progressive disease were significantly higher than those in normal donors , whereas FLT 3 ligand levels in patients who exhibited progressive disease were significantly lower than those in normal donors . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Hematopoietic stem cells mitogens SCF and flt 3 ligand ( FL ) were also mitogenic for fetal hepatocytes , but did not further enhance the effect of IL 6c on cell proliferation . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Cord blood derived purified CD34+ cells were grown in liquid medium supplemented with the cytokines stem cell factor , thrombopoietin , Flt 3 ligand , and IL 6 , and the chelator TEPA for the first 3 weeks and then for up to 11 weeks with cytokines alone . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
To explore the efficacy of the different combinations of stem cell factor ( SCF ) , FLT 3 ligand ( FL ) and interleukin ( IL ) 2 , 7 , 15 on the induction and expansion of cord blood ( CB ) derived CIK / NK cells in vitro , according to the different combinations of cytokines , combinations of cytokines were divided into 3 groups : group A ( SCF + IL 2 + IL 7 + IL 15 ) , group B ( SCF + FL + IL 2 + IL 7 + IL 15 ) and group C ( IL 2 + IL 7 + IL 15 as control group ) . ^^^ Expansion of CIK / NK cells from cord blood by using different combinations of stem cell factor , FLT 3 ligand and interleukin 2 , 7 , 15 in vitro ] . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Following transduction , Transduced cells were divided equally into four groups as follow : ( 1 ) No drug group , ( 2 ) AP 2018 alone group , ( 3 ) SCF + Flt 3 Ligand group , ( 4 ) AP 20187 + SCF + Flt 3 Ligand group . ^^^ RESULTS : Only the group of AP 20187 combined SCF and Flt 3 Ligand have a significant sustained outgrowth . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Several cytokines , especially stem cell factor ( SCF ) and FLT 3 ligand ( FL ) , have been identified as essential to produce large numbers of myeloid precursors and even to increase DC yield obtained by the action of granulocyte macrophage colony stimulating factor ( GM CSF ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
We report the results of experiments with CD 34 ( + ) cells from cord blood ( CB , n = 13 ) , bone marrow ( BM , n = 4 ) , and mobilized peripheral blood stem cells ( PBSC , n = 5 ) using two combinations of cytokines : ( A ) granulocyte colony stimulating factor ( G CSF ) , interleukin 3 ( IL 3 ) , interleukin 6 ( IL 6 ) , stem cell factor ( SCF ) , erythropoietin ( EPO ) , insulin like growth factor 1 ( IGF 1 ) , basic fibroblast growth factor ( FGF b ) and ( B ) combination A plus FLT 3 ligand ( FL ) and megakaryocyte growth and development factor ( PEG rhMGDF ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Here , we describe the characterization of purified CD 133 ( + ) progenitor cells from umbilical cord blood , a subset of CD 34 ( + ) hematopoietic progenitors that were grown in proliferation medium containing Flt 3 ligand , thrombopoietin and stem cell factor . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
To overcome this limitation , we developed an ex vivo expansion system for HSC , in which CB CD34+ cells are cultured on feeder cells ( HESS 5 cells ) in the presence of cytokines ( TPO , SCF and Flt 3 ligand ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
In the first step , CB CD34+ cells were expanded by stimulation with early acting cytokines such as stem cell factor ( SCF ) , flt 3 ligand ( FL ) , and thrombopoietin ( TPO ) to amplify the hematopoietic progenitor cells . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Ex vivo culture with the cytokines TPO , FLT 3 ligand , and SCF ( T / F / S ) plus IL 3 or IL 7 substantially increased the yield of transduced bone marrow ( BM ) CD 34 ( + ) cells purified from ADA SCID patients or healthy donors , compared to T / F / S alone . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Retroviral transduction of CD 34 ( + ) hemopoietic progenitor cells , during pre expansion by thrombopoietin , c kit ligand , and FLT 3 ligand , on recombinant fibronectin , but in the absence of FCS , resulted in gene expression by 20 30 % of the LCs . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
METHODS : Enriched CD34+ cells were cultured for 8 or 10 d in serum free media ( QBSF 60 or 10 Vivo 10 ) and four combinations of cytokines consisting of recombinant human pegylated megakaryocyte growth and development factor , stem cell factor , flt 3 ligand , G CSF , interleukin ( IL ) 6 , platelet derived growth factor ( PDGF ) , and IL 1beta . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
BM mononuclear cells from 3 dogs with melanoma and from 1 healthy dog were cultured for 12 days in media supplemented with recombinant human granulocyte macrophage colony stimulating factor , stem cell factor , tumor necrosis factor , and Flt 3 ligand . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
This study examined the effects of mesenchymal stem cells ( MSCs ) on the maintenance of CD34+ cells driven by aza D and TSA in culture with the combined cytokines of thrombopoietin , flt 3 ligand , stem cell factor , interleukin 3 , and interleukin 6 . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Within the first 6 hours post lethal dose radiation , there is no significant change of gene expression of the known radioprotective factors TPO , SCF , IL 3 , Flt 3 ligand , and SDF 1 . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
To evaluate the effect of cytokines , CD34+ cells were cultured with various cytokine combinations , such as stem cell factor ( SCF ) , flt 3 ligand ( FL ) , and thrombopoietin ( TPO ) with vascular endothelial growth factor ( VEGF ) , interleukin 1 beta , fibroblast growth factor basic ( FGF b ) as basic cytokines . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
For 3 days , HPC were maintained in serum free medium supplemented with a single cytokine ( SCF ) or a cytokine combination ( SCF , Flt 3 ligand , IL 6 ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
In media supplemented with thrombopoietin , stem cell factor , flt 3 ligand , and granulocyte colony stimulating factor , CD34+ cells , as well as CFU GM , BFU CFU E , CFU GEMM , and CFU MK , increased by 34 . 3 , 138 , 59 . 7 , 38 . 4 , and 86 . 0 fold , respectively . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Here , we report that the treatment of hES cells during EBs development with a combination of low dose hematopoietic cytokines , including stem cell factor ( SCF ) , Flt 3 ligand , vascular endothelial growth factor ( VEGF ) and human bone marrow stromal cells ( hBMSCs ) , generated cell clusters that contained 8 . 81 % KDR positive hemangioblasts , 9 . 94 % CD 34 positive hematopoietic stem cells and 25 . 7 % CD 45 positive mature hematopoietic cells , and expressed hematopoietic genes such as KDR , stem cell leukemia ( scl ) and runt related transcription factor 1 ( Runx 1 ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Mesenchymal stem cells support expansion of in vitro irradiated CD 34 ( + ) cells in the presence of SCF , FLT 3 ligand , TPO and IL 3 : potential application to autologous cell therapy in accidentally irradiated victims . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
When CD36+ erythroid precursor cells , generated from CD34+ cells with stem cell factor , flt 3 ligand , thrombopoietin , interleukin 3 , interleukin 6 , and erythropoietin , were cultured with an HDAC inhibitor FK 228 ( depsipeptide ) at a specified dose in the presence of erythropoietin , their differentiation was inhibited , as determined by the expression of CD 45 and glycophorin A . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
After 7 days of high density culture in microflasks , ( 10 ( 5 ) cells / ml , IMDM , FCS 10 % , thrombopoietin 10 ng / ml , flt 3 ligand 50 ng / ml , c kit ligand 20 ng / ml ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Our results demonstrated that CTCE 0214 synergized with thrombopoietin ( TPO ) , stem cell factor ( SCF ) , or flt 3 ligand ( FL ) on the survival of stem and progenitor cells in culture . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
The CD 34 ( + ) cells were plated for 10 days in presence of thrombopoietin ( TPO ) alone and combined with stem cell factor ( SCF ) , Flt 3 ligand ( FL ) , interleukin 3 ( IL 3 ) , IL 6 , and IL 11 . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
We found that hTERT was incapable of prolonging the replicative capacity of CB cells maintained under serum free conditions in the presence of stem cell factor , Flt 3 ligand , thrombopoietin , and interleukin 3 beyond 4 months ( n=3 ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
To explore whether HDAC inhibitors could have a beneficial role in human HSC transplantation , mobilized peripheral blood CD34+ cells were cultured with thrombopoietin mimetic peptide , flt 3 ligand , and c Kit ligand , without or with various HDAC inhibitors . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Briefly , CD133+ cells , purified from CB using Miltenyi Biotec ' s ( Bergisch Gladbach , Germany ) CliniMACS separation device and the anti CD 133 reagent , were cultured for 3 weeks in a clinical grade closed culture bag system , using the chelator based technology in combination with early acting cytokines ( SCF , thrombopoietin , IL 6 and FLT 3 ligand ) . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Therefore , administration of antiapoptotic cytokine combinations such as stem cell factor , Flt 3 ligand , thrombopoietin , and interleukin 3 ( 4F ) , may be important for multilineage recovery , particularly when these factors are administered early . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Cord blood derived lin ( ) cells were cultured for 8 days in serum free medium supplemented with stem cell factor , Flt 3 ligand , and thrombopoietin . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
CD34+CD38 and CD34+CD38+ cells from umbilical cord blood were analyzed before and after culture with thrombopoietin ( TPO ) , FLT 3 ligand ( FL ) and kit ligand ( KL ; or stem cell factor ) in different combinations : TPO + FL + KL , TPO + FL and TPO , at concentrations of 50 ng / mL each . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
We studied the effect of two different cytokine combinations kit ligand ( KL ) , interleukin 3 ( IL 3 ) , and Flt 3 ligand supplemented with thrombopoietin and IL 11 ( combination 1 ) or granulocyte / macrophage colony stimulating factor ( GM CSF ) and G CSF ( combination 2 ) for their ability to affect speed and extent of engraftment using limited numbers ( 5 10 10 ( 4 ) ) of murine bone marrow ( BM ) light density ( LD ) cells or their progeny expanded ex vivo in the presence one or the other cytokine combination for 6 days . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
The importance of early hematopoietic growth factors , such as c kit ligand and flt 3 ligand , and their synergy with IL 15 in the development of human NK cells in the bone marrow has permitted the investigation of novel cytokine combinations for optimizing in vivo expansion of NK cell in the clinic . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
UCB CD34+ cells were cultured for 10 days in serum free medium supplemented with recombinant human thrombopoietin , stem cell factor , flt 3 ligand and interleukin 3 . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
This method is based on immunoselection of CD34+ cells derived from mobilized peripheral blood of patients with metastatic breast cancer and expansion in the presence of flt 3 ligand , stem cell factor , interleukin 6 , and thrombopoietin . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Subsequently , we tested the reconstituting potential of cells cultured in FGF 1 + 2 with the addition of early acting stimulatory molecules , stem cell factor +interleukin 11 + Flt 3 ligand . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Human UCB CD 34 ( + ) cells were cultured on MS 5 mouse stroma cells in the presence of stem cell factor ( SCF ) , flt 3 ligand ( FL ) , and thrombopoietin ( TPO ) for 4 to 16 weeks . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
At doses that abrogated megakaryocytopoiesis , HC also inhibited the expansion of CD 34 ( + ) cells stimulated by stem cell factor , interleukin 3 and Flt 3 ligand and also induced apoptosis . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Lyn is renowned for its role in B cell antigen receptor and FcepsilonRI signaling ; however , it is becoming increasingly apparent that Lyn also functions in signal transduction from growth factor receptors including the receptors for GM CSF , IL 3 , IL 5 , SCF , erythropoietin , CSF 1 , G CSF , thrombopoietin and Flt 3 ligand . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Afterwards , a statistical analysis of the two level factorial designs revealed that in the presence of TPO , MK maturation was significantly stimulated by stem cell factor ( SCF ) , interleukin ( IL ) 6 , and IL 9 , whereas Flt 3 ligand ( FL ) had a positive effect only on the expansion of MK progenitors . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
Finally , reverse transcriptase polymerase chain reaction analysis showed that UC MSC had a cytokine spectrum very similar to that of BM MSC , including expression of the mRNA of stem cell factor , leukemia inhibitor factor , macrophage colony stimulating factor , Flt 3 ligand , interleukin 6 , vascular endothelial growth factor and stromal derived factor 1 , but UC MCS additionally expressed mRNA of granulocyte macrophage and granulocyte colony stimulating factors . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
In the present study , we investigated whether 10 irradiated hematopoietic stem cells can be induced to undergo megakaryocytopoiesis and thrombopoiesis in vitro using cytokine combinations that have been demonstrated to be effective for conferring increased survival on irradiated human CD 34 ( + ) megakaryocytic progenitor cells ( colony forming unit megakaryocytes ; CFU Meg ) , such as thrombopoietin ( TPO ) , interleukin 3 ( IL 3 ) , stem cell factor and FLT 3 ligand . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
We have investigated the influence of different hematopoietic growth factors , including granulocyte colony stimulating factor ( G CSF ) , stem cell factor ( SCF ) , Flt 3 ligand ( Flt 3L ) and thrombopoietin ( TPO ) , on the course of relapsing experimental autoimmune encephalomyelitis , a mouse model of multiple sclerosis . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
OBJECTIVES : The effect of stem cell factor ( SCF ) , flt 3 ligand ( FL ) , and interleukin ( IL ) 3 ( SF 3 ) in combination with hepatocyte growth factor ( HGF ) , thrombopoietin ( TPO ) , and Hyper IL 6 on maintenance and differentiation of early human peripheral blood derived progenitor cells was investigated . ^^^
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
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Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49771 and P21583	Pubmed	SVM Score :0.0
NA