| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| The observation that BALB / c mouse bone marrow derived mast cells developed with interleukin ( IL ) 10 and c kit ligand contain mMCP 9 transcript , whereas those developed with IL 3 do not , indicates that the expression of this particular chymase is regulated by the cytokine microenvironment . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| After 3 wk of culture in the presence of c kit ligand , IL 16 induced the progenitors residing in the blood of normal individuals to increase their expression of chymase and tryptase about 20 fold . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| In contrast , recombinant human Kit ligand induced the differentiation of mast cells that were tryptase positive but negative for chymase , carboxypeptidase , and Fc epsilon RI . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| We conclude that chronic stimulation of the KIT kit ligand axis does not irrevocably commit mast cells to a chymase positive or chymase negative phenotype . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| This conclusion was supported by the fact that the majority of mast cells developed by c kit ligand contained only tryptase in their granules , whereas those developed in the cocultures contained both tryptase and chymase . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| Mast cells expressing chymase but not tryptase can be derived by culturing human progenitors in conditioned medium obtained from a human mastocytosis cell strain with c kit ligand . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| Kit ligand / stem cell factor induced synthesis of the chymase MMCP 4 and the tryptases MMCP 6 and MMCP 7 and increased the fraction of c kit+ and FcepsilonRI+ L138 . 8A cells to > 70 % . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| RESULTS : The peripheral blood contained 1 % to 3 % nonmetachromatic mononuclear cells with eccentric nuclei that expressed the mast cell proteases , tryptase and carboxypeptidase A , along with c kit , stem cell factor ( SCF ) , and high affinity IgE receptor ( Fc epsilon RI ) , but not chymase . ^^^ Similar mononuclear cells colocalized in the spleen and lymph nodes with mature , metachromatic mast cells that expressed tryptase , chymase , carboxypeptidase A , c kit , SCF , and Fc epsilon RI . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| In this study , we demonstrate that human mast cell chymase , a chymotrypsin like protease , cleaves SCF at a novel site . ^^^ Because mast cells express the KIT protein , the receptor for SCF , and respond to SCF by proliferation and degranulation , this observation identifies a possible feedback loop in which chymase released from mast cell secretory granules may solubilize SCF bound to the membrane of surrounding stromal cells . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| Human cultured mast cells ( HCMCs ) grown from cord blood mononuclear cells in the presence of stem cell factor ( SCF ) and interleukin 6 ( IL 6 ) expressed tryptase but no or low chymase in their cytoplasm . ^^^ Taken together , immature HCMCs grown only with SCF and IL 6 expressed tryptase with no or a low amount of chymase , and addition of IL 4 promoted cell maturation together with the expression of both tryptase and a high amount of chymase . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| We also addressed the question whether mast cell chymase , a chymotrypsin like protease , also present in the secretory granules of human mast cells cleaves SCF at the peptide bound between Phe 158 and Met 159 . ^^^ SCF 1 166 is rapidly cleaved by chymase and other proteases to several SCF species . . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| After 4 wk of culture in the presence of recombinant stem cell factor ( SCF ) , interleukin ( IL ) 6 , and IL 10 , the cells were characterized as hPrMCs based upon their uniform surface expression of c kit and CD 13 , low level expression of FcinRIalpha , absence of CD 14 and CD 16 expression , and immunoreactivity for MC chymase in > 80 % , and about half were immunoreactive for tryptase and metachromatic with toluidine blue . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| HPLC and electrospray mass spectrometry ( ES / MS ) analysis of recombinant human SCF 1 166 ( 18 , 656 . 9 + / 0 . 9 Da ) treated with chymase showed a polypeptide of 17 , 977 . 1 + / 0 . 6 Da and a minor component of 697 . 4 + / 0 . 1 Da generated by specific cleavage at Phe 159 . ^^^ SCF 1 166 is rapidly and specifically cleaved to SCF 1 159 by chymase , which retains its biological effect on mast cells . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| MC were visualized in terms of metachromasia , reflecting glycosaminoglycan content , and immunohistochemically , visualizing tryptase , chymase and IL 6 as well as the surface markers CD 117 and SCF . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| Although overnight incubation of the eosinophils in either culture medium or in phorbol 12 myristate 13 acetate calcium ionophore did not cause the secretion of SCF , the addition of chymase induced SCF release . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| Furthermore , SCF by itself supports the predominance of the tryptase / chymase double positive mast cell subtype MCTC whereas the addition of IL 4 supports the chymase negative MCT subtype . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemical analysis revealed that the intradermal injection of chymase reduces expression of stem cell factor ( SCF ) on surface of the skin keratinocytes . ^^^ In addition , incubation of human keratinocytes with chymase in vitro resulted in release of SCF into the culture medium . ^^^ Since soluble SCF is thought to regulate mast cell number , the chymase induced mast cell accumulation may occur via the ability of chymase to process membrane bound SCF on the epidermal keratinocytes . . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| Most of the cells , cultured in the presence of SCF and IL 6 for 10 weeks , expressed c kit and contained a significant amount of histamine and tryptase and a low amount of chymase . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| Mast cells , identified by tryptase and chymase immunostaining on consecutive tissue sections , showed immunoreactivity for SCF and c kit in both normal and cancerous specimens and their number was significantly increased ( p = 0 . 03 ) in pancreatic cancer compared with the normal pancreas . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| Human cord blood CD34+ cells differentiate and grow into mast cells in the presence of stem cell factor ( SCF ) and IL 6 , causing increases in cell size , frequency of chymase positive cells , and intracellular histamine levels when compared with cells treated with SCF alone . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| Duodenal biopsies from 5 cholerae O 1 infected patients showed upregulation of myeloperoxidase , lactoferrin , PGHS 1 , SCF , tryptase , tumour necrosis factor alpha , alpha defensin , and eotaxin during the acute stage and chymase , interleukin 3 and major basic protein during convalescence . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| In parallel , TGF beta 1 increased the percentage of connective tissue type MC expressing tryptase and chymase while downregulating expression of the receptors for IgE and SCF . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| Expression of the serine proteases tryptase and chymase , as well as KIT protein , the receptor for stem cell factor ( SCF ) , has been demonstrated in mast cells . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| The MCs expressed the stem cell factor receptor CD 117 and exhibited a variable tryptase immunoreactivity , but lacked chymase . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| In order to explore a possible involvement of mast cells during human wound healing , we studied sections from scars ( 4 369 d old ) ( N = 20 ) and normal skin ( N = 10 ) for mast cell specific tryptase and chymase by enzyme histochemistry , for the stem cell factor receptor c Kit and the melanosomal marker TA 99 by immunohistochemistry , and for simultaneous c Kit expression and avidin fluorescence by double staining . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| The chymase protein expression increased by culture duration and was enhanced by cytokines , such as a high concentration of stem cell factor or IL 4 . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : The combination of recombinant human stem cell factor ( rhSCF ) , rh interleukin ( IL ) 6 and rhIL 10 was reported to be optimal for mast cell development from cord blood progenitors and to induce chymase expression in all such mast cells earlier in their development than tryptase . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| Addition of exogenous stem cell factor ( up to 200 ng / ml ) to the SM culture could not prevent the decay in tryptase and chymase positive cells . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| Homologues of MMC that express and constitutively secrete abundant amounts of the granule chymase , mouse mast cell protease 1 ( mMCP 1 ) , can be generated in vitro from bone marrow cultures supplemented with interleukins 3 and 9 , stem cell factor and transforming growth factor beta 1 . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| These cells were evaluated by immunocytochemistry with antitryptase and antichymase mAbs ; for chymase and tryptase mRNA by real time RT PCR ; for conversion of MC ( T ) to MC ( TC ) cells during cell culture with recombinant human stem cell factor and recombinant human IL 6 ; and for degranulation and leukotriene C 4 ( LTC 4 ) secretion when stimulated with anti FcepsilonRI , substance P , C5a , and compound 48 / 80 . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| After 5 7 weeks of culture with recombinant canine stem cell factor ( rcSCF ) , greater than 90 % of the cell population consisted of mast cells as evidenced by staining with Wright ' s Giemsa , as well as production of chymase , tryptase , IL 8 and MCP 1 . ^^^ |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21583 and P23946 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| This conclusion was supported by the fact that the majority of mast cells developed by c kit ligand contained only tryptase in their granules , whereas those developed in the cocultures contained both tryptase and chymase . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| In contrast , recombinant human Kit ligand induced the differentiation of mast cells that were tryptase positive but negative for chymase , carboxypeptidase , and Fc epsilon RI . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| Mast cells expressing chymase but not tryptase can be derived by culturing human progenitors in conditioned medium obtained from a human mastocytosis cell strain with c kit ligand . ^^^ The conditioned medium and kit ligand promoted the development of distinct MC subtypes from bone marrow and umbilical cord blood progenitors . kit ligand induced MC from umbilical cord blood predominantly of two immunophenotypes , MCT positive for tryptase but negative for chymase , and MCTC positive for tryptase and chymase . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| RESULTS : The peripheral blood contained 1 % to 3 % nonmetachromatic mononuclear cells with eccentric nuclei that expressed the mast cell proteases , tryptase and carboxypeptidase A , along with c kit , stem cell factor ( SCF ) , and high affinity IgE receptor ( Fc epsilon RI ) , but not chymase . ^^^ Similar mononuclear cells colocalized in the spleen and lymph nodes with mature , metachromatic mast cells that expressed tryptase , chymase , carboxypeptidase A , c kit , SCF , and Fc epsilon RI . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| The observation that BALB / c mouse bone marrow derived mast cells developed with interleukin ( IL ) 10 and c kit ligand contain mMCP 9 transcript , whereas those developed with IL 3 do not , indicates that the expression of this particular chymase is regulated by the cytokine microenvironment . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| Human cultured mast cells ( HCMCs ) grown from cord blood mononuclear cells in the presence of stem cell factor ( SCF ) and interleukin 6 ( IL 6 ) expressed tryptase but no or low chymase in their cytoplasm . ^^^ Taken together , immature HCMCs grown only with SCF and IL 6 expressed tryptase with no or a low amount of chymase , and addition of IL 4 promoted cell maturation together with the expression of both tryptase and a high amount of chymase . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| We conclude that chronic stimulation of the KIT kit ligand axis does not irrevocably commit mast cells to a chymase positive or chymase negative phenotype . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| The MCs expressed the stem cell factor receptor CD 117 and exhibited a variable tryptase immunoreactivity , but lacked chymase . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| We also addressed the question whether mast cell chymase , a chymotrypsin like protease , also present in the secretory granules of human mast cells cleaves SCF at the peptide bound between Phe 158 and Met 159 . ^^^ SCF 1 166 is rapidly cleaved by chymase and other proteases to several SCF species . . ^^^ Novel autocrine and paracrine loops of the stem cell factor / chymase network . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| In this study , we demonstrate that human mast cell chymase , a chymotrypsin like protease , cleaves SCF at a novel site . ^^^ Because mast cells express the KIT protein , the receptor for SCF , and respond to SCF by proliferation and degranulation , this observation identifies a possible feedback loop in which chymase released from mast cell secretory granules may solubilize SCF bound to the membrane of surrounding stromal cells . ^^^ Chymase cleavage of stem cell factor yields a bioactive , soluble product . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| After 4 wk of culture in the presence of recombinant stem cell factor ( SCF ) , interleukin ( IL ) 6 , and IL 10 , the cells were characterized as hPrMCs based upon their uniform surface expression of c kit and CD 13 , low level expression of FcinRIalpha , absence of CD 14 and CD 16 expression , and immunoreactivity for MC chymase in > 80 % , and about half were immunoreactive for tryptase and metachromatic with toluidine blue . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| HPLC and electrospray mass spectrometry ( ES / MS ) analysis of recombinant human SCF 1 166 ( 18 , 656 . 9 + / 0 . 9 Da ) treated with chymase showed a polypeptide of 17 , 977 . 1 + / 0 . 6 Da and a minor component of 697 . 4 + / 0 . 1 Da generated by specific cleavage at Phe 159 . ^^^ SCF 1 166 is rapidly and specifically cleaved to SCF 1 159 by chymase , which retains its biological effect on mast cells . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| In order to explore a possible involvement of mast cells during human wound healing , we studied sections from scars ( 4 369 d old ) ( N = 20 ) and normal skin ( N = 10 ) for mast cell specific tryptase and chymase by enzyme histochemistry , for the stem cell factor receptor c Kit and the melanosomal marker TA 99 by immunohistochemistry , and for simultaneous c Kit expression and avidin fluorescence by double staining . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| MC were visualized in terms of metachromasia , reflecting glycosaminoglycan content , and immunohistochemically , visualizing tryptase , chymase and IL 6 as well as the surface markers CD 117 and SCF . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| The chymase protein expression increased by culture duration and was enhanced by cytokines , such as a high concentration of stem cell factor or IL 4 . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| Kit ligand / stem cell factor induced synthesis of the chymase MMCP 4 and the tryptases MMCP 6 and MMCP 7 and increased the fraction of c kit+ and FcepsilonRI+ L138 . 8A cells to > 70 % . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| Although overnight incubation of the eosinophils in either culture medium or in phorbol 12 myristate 13 acetate calcium ionophore did not cause the secretion of SCF , the addition of chymase induced SCF release . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| Furthermore , SCF by itself supports the predominance of the tryptase / chymase double positive mast cell subtype MCTC whereas the addition of IL 4 supports the chymase negative MCT subtype . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : The combination of recombinant human stem cell factor ( rhSCF ) , rh interleukin ( IL ) 6 and rhIL 10 was reported to be optimal for mast cell development from cord blood progenitors and to induce chymase expression in all such mast cells earlier in their development than tryptase . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemical analysis revealed that the intradermal injection of chymase reduces expression of stem cell factor ( SCF ) on surface of the skin keratinocytes . ^^^ In addition , incubation of human keratinocytes with chymase in vitro resulted in release of SCF into the culture medium . ^^^ Since soluble SCF is thought to regulate mast cell number , the chymase induced mast cell accumulation may occur via the ability of chymase to process membrane bound SCF on the epidermal keratinocytes . . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| After 3 wk of culture in the presence of c kit ligand , IL 16 induced the progenitors residing in the blood of normal individuals to increase their expression of chymase and tryptase about 20 fold . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| Most of the cells , cultured in the presence of SCF and IL 6 for 10 weeks , expressed c kit and contained a significant amount of histamine and tryptase and a low amount of chymase . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| Mast cells , identified by tryptase and chymase immunostaining on consecutive tissue sections , showed immunoreactivity for SCF and c kit in both normal and cancerous specimens and their number was significantly increased ( p = 0 . 03 ) in pancreatic cancer compared with the normal pancreas . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| Human cord blood CD34+ cells differentiate and grow into mast cells in the presence of stem cell factor ( SCF ) and IL 6 , causing increases in cell size , frequency of chymase positive cells , and intracellular histamine levels when compared with cells treated with SCF alone . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| Addition of exogenous stem cell factor ( up to 200 ng / ml ) to the SM culture could not prevent the decay in tryptase and chymase positive cells . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| Homologues of MMC that express and constitutively secrete abundant amounts of the granule chymase , mouse mast cell protease 1 ( mMCP 1 ) , can be generated in vitro from bone marrow cultures supplemented with interleukins 3 and 9 , stem cell factor and transforming growth factor beta 1 . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| Duodenal biopsies from 5 cholerae O 1 infected patients showed upregulation of myeloperoxidase , lactoferrin , PGHS 1 , SCF , tryptase , tumour necrosis factor alpha , alpha defensin , and eotaxin during the acute stage and chymase , interleukin 3 and major basic protein during convalescence . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| These cells were evaluated by immunocytochemistry with antitryptase and antichymase mAbs ; for chymase and tryptase mRNA by real time RT PCR ; for conversion of MC ( T ) to MC ( TC ) cells during cell culture with recombinant human stem cell factor and recombinant human IL 6 ; and for degranulation and leukotriene C 4 ( LTC 4 ) secretion when stimulated with anti FcepsilonRI , substance P , C5a , and compound 48 / 80 . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| In parallel , TGF beta 1 increased the percentage of connective tissue type MC expressing tryptase and chymase while downregulating expression of the receptors for IgE and SCF . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| Expression of the serine proteases tryptase and chymase , as well as KIT protein , the receptor for stem cell factor ( SCF ) , has been demonstrated in mast cells . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| After 5 7 weeks of culture with recombinant canine stem cell factor ( rcSCF ) , greater than 90 % of the cell population consisted of mast cells as evidenced by staining with Wright ' s Giemsa , as well as production of chymase , tryptase , IL 8 and MCP 1 . ^^^ |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23946 and P21583 |
Pubmed |
SVM Score :0.0 |
| NA |
|