Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.92685376
The W locus encodes the c kit proto oncogene product , a cell surface receptor with protein tyrosine kinase activity , and the Sl locus encodes its ligand , a hemopoietic cytokine known variously as Steel factor ( SLF ) , mast cell growth factor , stem cell factor , and Kit ligand . 0.92685376^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.62495508
Mast cell growth factor ( MGF ) is encoded by the murine steel ( Sl ) locus and is a ligand for the tyrosine kinase receptor protein encoded by the proto oncogene c kit at the murine dominant white spotting ( W ) locus . 0.62495508^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.54992158
Surprisingly , activation of KIT with mast cell growth factor ( MGF ) in melanoma cells produced biological responses opposite to those elicited in normal melanocytes . 0.54992158^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :1.1731837
MGF was shown to be a ligand for c kit by cross linking 125I labeled MGF to c kit expressing cells with subsequent immunoprecipitation of the complex with antiserum specific for the C terminus of c kit . 1.1731837^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.51502741
In murine cells , hSCF behaves as an antagonist to normal SCF function , due to interference with the interaction between endogenous murine SCF and its receptor , c kit , encoded by the dominant white spotting ( W ) gene . 0.51502741^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.76241955
Studies in glioma cell lines as well as different tumor types suggest the possibility of intracellular interactions of c kit with SCF . 0.76241955^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.51128346
The ICCs express the c kit proto oncogene encoding a type 3 tyrosine kinase ( KIT ) receptor , a ligand that is known as stem cell factor ( SCF ) . 0.51128346^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :1.2069264
MATERIALS AND METHODS : We characterized the interactions between c Kit and PI 3 kinase ( p 85 ) after stimulation with SCF ( stem cell factor ) as well as the regulation of SHP 1 and SHP 2 associated with Kit in this cell line . 1.2069264^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.51765618
Recombinant human stem cell factor ( SCF ) , the ligand for c kit , has been shown to stimulate increased numbers of hematopoietic progenitor cells of multiple types to circulate in the blood of baboons , but it was not known if the cells stimulated to circulate by SCF contained cells capable of engrafting and rescuing lethally irradiated baboons . 0.51765618^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.64311916
Interactions between stem cell factor ( SCF ) and its receptor , c Kit , are important for development of hematopoietic , melanocytes , and germ cells . 0.64311916^^^ Interactions between stem cell factor and c Kit are required for intestinal immune system homeostasis . 0.56167666^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.61010946
Tyrosine phosphorylation and activation of the c kit product with the ligand for c kit ( stem cell factor : SCF ) results in proliferation of some human leukemia cell lines , such as M07E , and blast cells in a substantial fraction of AML cases . 0.61010946^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.67077923
Interactions between c kit and stem cell factor are not required for B cell development in vivo . 0.67077923^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.53293063
Interaction between stem cell factor ( SCF ) and its receptor , c kit , was demonstrated to be important for stroma cell dependent growth by anti c kit neutralizing monoclonal antibody ( mAb ) inhibition experiments . 0.53293063^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.53485209
By analysis of Ba / F3 cells retrovirally transduced to express c Kit receptors , phenylalanine substitution of c Kit tyrosine residue 569 was shown to be associated with disruption of c Kit SHP 1 binding and induction of hyperproliferative responses to stem cell factor . 0.53485209^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.88866476
Interaction of c kit with its ligand stem cell factor induces dimerization , receptor phosphorylation , and signal transduction . 0.88866476^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.7651697
C kit encodes a transmembrane protein with intrinsic tyrosine kinase activity , which functions as the receptor for stem cell factor . 0.7651697^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.63153691
Stem cell factor ( SCF ) binds and activates the receptor tyrosine kinase c Kit , and this interaction is critical for normal hematopoiesis . 0.63153691^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.57483102
Here , we show that stem cell factor ( SCF ) , the ligand for KIT , induces the interaction between KIT and Cbl proteins and their mutual degradation . 0.57483102^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.6413563
Normally KIT is activated ( phosphorylated ) by binding of its ligand , the stem cell factor . 0.6413563^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
TRP 2 / DT , a new early melanoblast marker , shows that steel growth factor ( c kit ligand ) is a survival factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Their enhanced ability to support DW 34 cell growth was not caused by cytokines , including IL 6 , IL 7 , and c kit ligand , although exogenous IL 7 could augment the growth supporting ability . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Canine stem cell factor ( c kit ligand ) supports the survival of hematopoietic progenitors in long term canine marrow culture . ^^^ The cDNA for canine stem cell factor ( cSCF , c kit ligand ) was cloned and expressed in Escherichia coli . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Here we use a recently identified hemopoietic growth factor , stem cell factor ( SCF ) ( a form of the c kit ligand ) , and a transplant model of thymocyte regeneration to assess the effect of SCF on the in vivo generation of prethymic , thymocyte progenitor cell activity . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
For B cells , IL 7 and c kit ligand ( KL ) are clearly important but so are as yet undefined stromal cell derived activities . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Effects of the stem cell factor , c kit ligand , on human megakaryocytic cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Role of c kit ligand in the expansion of human hematopoietic progenitor cells . ^^^ To test the hypothesis that the c kit ligand plays an important role in the regulation of early events occurring during human hematopoiesis , we determined the effect of a recombinant form of c kit ligand , termed mast cell growth factor ( MGF ) , on the high proliferative potential colony forming cell ( HPP CFC ) and the cell responsible for initiating long term hematopoiesis in vitro ( LTBMIC ) . ^^^ These data indicate that the c kit ligand synergistically interacts with a number of cytokines to directly augment the proliferative capacity of primitive human hematopoietic progenitor cells . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Human mast cell growth factor ( MGF , a c kit ligand ) and colony stimulating factors ( Epo , GM CSF , G CSF , IL 3 ) were assessed in the absence or presence of serum for stimulation in semi solid medium of single CD 34 , CD 34 HLA DR+ , or CD 34 HLA DR+CD33 cells sorted per microtiter well . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Differential expression of secretory granule proteases in mouse mast cells exposed to interleukin 3 and c kit ligand . ^^^ When bone marrow derived MC ( BMMC ) were developed with recombinant interleukin 3 ( rIL 3 ) and maintained with this cytokine in the absence or presence of recombinant c kit ligand ( rKL ) , they remained safranin , produced almost no 35S labeled heparin proteoglycans , and contained greater levels of mouse MC protease ( MMCP ) 5 mRNA and mast cell carboxypeptidase A ( MC CPA ) mRNA than MMCP 6 mRNA . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The addition of c kit ligand ( KL ) increased both FP stimulated CFU MK and BFU MK derived colony numbers but only BFU MK derived colony size . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The proliferative capacity of mouse connective tissue type mast cells ( CTMC ) was analyzed by using a newly discovered c kit ligand , termed stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Here , we show that the c kit ligand ( KL ) , a recently identified stem cell growth factor , at concentrations 10 100 times lower than that required to promote cell proliferation , enhances the release of histamine and leukotriene C 4 in response to IgE receptor crosslinking of human lung MC . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The rat c kit ligand , stem cell factor , induces c kit receptor dependent mouse mast cell activation in vivo . ^^^ Moreover , the c kit ligand SCF can induce the proliferation and maturation of normal mast cells in vitro or in vivo , and also can result in repair of the mast cell deficiency of Sl / Sld mice in vivo . ^^^ These findings represent the first demonstration in vivo that a c kit ligand can result in the functional activation of any cellular lineage expressing the c kit receptor , and suggest that interactions between the c kit receptor and its ligand may influence mast cell biology through complex effects on proliferation , maturation , and function . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Thymic hyperplasia in transgenic mice caused by immortal epithelial cells expressing c kit ligand . ^^^ Most importantly , Epi A 1 cells also express the IL 4 receptor and the c kit ligand ( KL ) , a factor that , in concert with commitment factors , channels progenitors into hemopoietic lineages . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The c kit ligand , KL , and its receptor , the proto oncogene c kit are encoded , respectively , at the steel ( Sl ) and white spotting ( W ) loci of the mouse . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Analysis of these interactions revealed that a single ECM protein , thrombospondin , in conjunction with a single cytokine ( e . g . , c kit ligand ) , constitutes a developmental signal that synergistically modulates hematopoietic stem cell function . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Plateau levels of rr stem cell factor ( rrSCF ) , a c kit ligand , used alone also had negligible clonogenic capacity , but when combined with the low level combination of the other five factors produced total colony and HPP CFC growth approaching that produced by all factors at plateau levels . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
A c kit ligand , recombinant human stem cell factor , mediates reversible expansion of multiple CD34+ colony forming cell types in blood and marrow of baboons . ^^^ The results of these studies suggest a role for the c kit ligand in stimulating the expansion of multiple CFC types in blood and marrow for potential therapeutic purposes . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In the presence of interleukin 3 ( IL 3 ) , IL 6 , erythropoietin , and mast cell growth factor ( a c kit ligand ) , cell numbers increased approximately 10 ( 6 ) fold over a period of 4 wk , and the percentage of cells that expressed transferrin receptors ( CD 71 ) increased from less than 0 . 1 % at day 0 to greater than 99 % at day 14 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We describe the effect of soluble c kit ligand ( stem cell factor , SCF ) on highly purified CD 34 positive hemopoietic progenitors from human umbilical cord blood . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
A mouse antihuman monoclonal IgG2a antibody , termed stem cell receptor 1 ( SR 1 ) , specific for a determinant of the c kit ligand receptor ( KR ) , was used as an immunologic probe to analyze KR expression by human bone marrow hematopoietic progenitor cells . ^^^ Long term bone marrow cultures initiated with CD34+ HLA DR SR 1+ but not CD34+ HLA DR SR 1 cells , which were repeatedly supplemented with c kit ligand ( KL ) and interleukin 3 , generated assayable progenitor cells of at least 2 lineages for 10 weeks . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The present studies analyzing stromal cytokine messenger RNA ( mRNA ) production by standard Northern blot analysis show constitutive production of mRNAs for CSF 1 , GM CSF , granulocyte CSF ( G CSF ) , c kit ligand ( KL ) , and interleukin 6 ( IL 6 ) , but not IL 3 , IL 4 , or IL 5 by 3 week irradiated or nonirradiated murine Dexter stromal cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Furthermore , cells of subclones of the PA 6 clone that were incapable of supporting long term hemopoiesis expressed mRNA for the c kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Effects of human stem cell factor ( c kit ligand ) on proliferation of myeloid leukemia cells : heterogeneity in response and synergy with other hematopoietic growth factors . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We investigated the stimulative effect of c kit ligand ( KL ) on the erythroid colony forming abilities of bone marrow cells from 17 patients with MDS . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Our results show that in the presence of IL 3 or c kit ligand ( KL ) , IL 11 has profound stimulatory effects on primitive multilineage hematopoietic progenitors , pre CFC ( multi ) , as well as on precursors representing various stages of erythroid differentiation observable in vitro , including CFC ( multi ) , BFU E , and CFU E . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Steel factor ( SF ) ( also called stem cell factor , mast cell growth factor , or c kit ligand ) is a recently cloned hemopoietic growth factor that is produced by bone marrow stromal cells , fibroblasts , and hepatocytes . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Interaction of human bone marrow fibroblasts with megakaryocytes : role of the c kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In this report , we have examined the expression of these integrin chain genes by mature connective tissue mast cells ( CTMC ) and by bone marrow derived mast cells ( BMMC ) differentiated from bone marrow precursor cells in the presence of interleukin ( IL ) 3 and / or the c kit ligand ( also known as mast cell growth factor and stem cell growth factor ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The effects of the c kit ligand ( stem cell factor [ SCF ] ) on the development of a highly enriched population of granulocyte macrophage colony forming cells ( GM CFC ) were assessed . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
IL 6 alone is poorly active on the growth of stem cell populations , but acts in synergy with stem cell factor ( c kit ligand ) to expand the committed myeloid progenitor compartments but not the megakaryocyte progenitors . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Effect of mast cell growth factor ( c kit ligand ) on clonogenic leukemic precursor cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In the accompanying article we report the identification and purification of mast cell growth factor ( MGF ) , a c kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Based on the defective proliferative response of W mast cells in the 3T3 fibroblast co culture system it had been proposed that fibroblasts produce the c kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Influence of murine mast cell growth factor ( c kit ligand ) on colony formation by mouse marrow hematopoietic progenitor cells . ^^^ Purified natural and recombinant murine mast cell growth factor ( MGF , a c kit ligand ) were evaluated alone and in combination with other cytokines for effects in vitro on colony formation by multipotential ( CFU GEMM ) , erythroid ( BFU E ) and granulocyte macrophage ( CFU GM ) progenitor cells from BDF 1 mouse bone marrow . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The expression of the mRNA for SLF ( the c kit ligand ) , a product of the `` steel ' ' locus , has been investigated in postnatal mouse testis and homogeneous populations of testicular cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Purified natural ( n ) and recombinant ( r ) murine ( mu ) mast cell growth factor ( MGF , a c kit ligand ) were evaluated alone and in combination with r human ( hu ) erythropoietin ( Epo ) , rhu granulocyte macrophage colony stimulating factor ( rhuGM CSF ) , rhuG CSF , and / or rhuM CSF for effects in vitro on colony formation by multipotential ( colony forming unit granulocyte , erythroid , monocyte , megakaryocyte [ CFU GEMM ] ) , erythroid ( burst forming unit erythroid [ BFU E ] ) and granulocyte macrophage ( CFU GM ) progenitor cells from normal human bone marrow . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Steel Dickie mutation encodes a c kit ligand lacking transmembrane and cytoplasmic domains . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The rat c kit ligand , stem cell factor , induces the development of connective tissue type and mucosal mast cells in vivo . ^^^ Recent work indicates that W encodes the c kit tyrosine kinase receptor , whereas S 1 encodes a c kit ligand that we have designated stem cell factor ( SCF ) . ^^^ Recombinant or purified natural forms of the c kit ligand induce proliferation of certain mast cell populations in vitro , and injection of recombinant SCF permits mast cells to develop in mast cell deficient WCB6F1 S1 / S1d mice . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Induction of mast cell proliferation , maturation , and heparin synthesis by the rat c kit ligand , stem cell factor . ^^^ We investigated the effects of a newly recognized multifunctional growth factor , the c kit ligand stem cell factor ( SCF ) , on mouse mast cell proliferation and phenotype . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
CD 45 cell surface antigens are linked to stimulation of early human myeloid progenitor cells by interleukin 3 ( IL 3 ) , granulocyte / macrophage colony stimulating factor ( GM CSF ) , a GM CSF / IL 3 fusion protein , and mast cell growth factor ( a c kit ligand ) . ^^^ A possible link was evaluated between expression of CD 45 antigens on human myeloid progenitor cells ( MPC ) ( colony forming unit granulocyte / macrophage [ CFU GM ] , burst forming unit erythroid [ BFU E ] , and colony forming unit granulocyte / erythroid / macrophage / megakaryocyte [ CFU GEMM ] ) and regulation of MPC by colony stimulating factors ( CSF ) ( interleukin 3 [ IL 3 ] , GM CSF , G CSF , M CSF , and erythropoietin [ Epo ] ) , a GM CSF / IL 3 fusion protein , and mast cell growth factor ( MGF ; a c kit ligand ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
More recently , the Sl locus has been shown to encode a ligand for c kit , which is variously known as mast cell growth factor ( MGF ) , stem cell growth factor and c kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Zsebo et al identified and cloned a gene for a cytokine termed stem cell factor ( SCF ) in the medium conditioned by buffalo rat liver cells , and this cytokine proved to be c kit ligand . ^^^ However , rrSCF supported formation of a significant number of neutrophil and neutrophil / macrophage colonies from pooled blast cells , indicating that rrSCF is able to support directly the proliferation of progenitors in neutrophil / monocyte lineages . c kit ligand may play important roles in adult hematopoiesis . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mutations in the murine Dominant White Spotting ( W ) and Steel genes , which respectively encode the c kit tyrosine kinase receptor and the c kit ligand ( or Steel factor ) , impair the development of primordial germ cells ( PGCs ) in vivo , as well as haematopoietic stem cells and neural crest derived melanoblasts . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We investigated the expression , degree of phosphorylation , and activation of the proto oncogene c kit product before and after stimulation with the c kit ligand in a human factor dependent myeloid leukemia cell line , MO7E . ^^^ An immunoblot technique with a monoclonal antibody specific for phosphotyrosine , showed that there was rapid , dose dependent tyrosine phosphorylation of the c kit product in response to murine c kit ligand . ^^^ Furthermore , the murine c kit ligand increased autokinase activity of the c kit product in vitro . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Effect of c kit ligand on in vitro human megakaryocytopoiesis . ^^^ An evaluation of the effects of a recombinant , soluble form of the c kit ligand alone and in combination with either granulocyte macrophage colony stimulating factor ( GM CSF ) or interleukin 3 ( IL 3 ) on the regulation of human megakaryocytopoiesis was performed using a serum depleted clonal assay system and a long term bone marrow culture system . ^^^ The effects of the c kit ligand on the primitive megakaryocyte ( MK ) progenitor cell , the burst forming unit megakaryocyte ( BFU MK ) , and the more differentiated colony forming unit megakaryocyte ( CFU MK ) were determined . ^^^ The c kit ligand alone had no megakaryocyte colony stimulating activity ( MK CSA ) but was capable of augmenting the MK CSA of both GM CSF and IL 3 . ^^^ The range of synergistic interactions of c kit ligand varied with the class of MK progenitor cell assayed . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To define the cellular targets for c kit ligand ( KL ) and to study their functional properties and composite antigenic profile , we isolated cells expressing c kit receptor ( KR ) from bone marrow ( BM ) , peripheral blood , and fetal liver ( FL ) using immunoadherence to a recently obtained antibody ( SR 1 ) against the human KR . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Identification of the c kit ligand : end of the road for understanding aplastic anemia in steel mutant mice . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mast cell growth factor ( c kit ligand ) enhances cytokine stimulation of proliferation of the human factor dependent cell line , M07e . ^^^ Murine mast cell growth factor ( muMGF ) , a c kit ligand , has additive to greater than additive effects on in vitro colony formation of murine and human myeloid progenitor cells stimulated with erythropoietin , granulocyte macrophage colony stimulating factor ( GM CSF ) , and / or interleukin ( IL ) 3 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mast cell growth factor ( c kit ligand ) supports the growth of human multipotential progenitor cells with a high replating potential . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Both stem cells and mast cells express c kit and proliferate after exposure to c kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Subsequent experiments testing the effects of various growth factors on transformation of bone marrow cells by the 5 ski oncogene product alone identified the avian c kit ligand ( stem cell factor ; SCF ) as being able to co operate with the 5 ski protein to cause transformation of chicken hematopoietic cells of both myeloid and erythroid lineages . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) or c kit ligand is a growth factor cytokine produced by stromal cells that is known to influence mast cell proliferation and differentiation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Comparative analysis of signaling pathways between mast cell growth factor ( c kit ligand ) and granulocyte macrophage colony stimulating factor in a human factor dependent myeloid cell line involves phosphorylation of Raf 1 , GTPase activating protein and mitogen activated protein kinase . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Studies on the efficacy of mast cell growth factor ( c kit ligand ) in vitro as well as in vivo . ^^^ We tested the ability of recombinant human ( rhu ) mast cell growth factor ( MGF ) , also known as c kit ligand , to stimulate the colony formation of human bone marrow cells in semisolid medium alone and in combination with rhu erythropoietin ( EPO ) , rhu Interleukin 3 ( IL 3 ) , rhu granulocyte colony stimulating factor ( G CSF ) and rhu granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Recombinant human stem cell factor , a c kit ligand , stimulates hematopoiesis in primates . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Similar results were observed regardless of the early acting cytokines used : human rGM CSF plus human rIL 3 , and two recently described potent cytokines , a genetically engineered human rGM CSF / IL 3 fusion protein and MGF , a c kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In two companion papers , we present the sequences of partial SCF cDNAs , identify SCF as a c kit ligand , and map the SCF gene to the Sl locus of the mouse . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Based on in vitro studies , mast cell growth factor ( MGF ; also known as steel factor , stem cell factor , and c kit ligand ) has been implicated as an important hematopoietic regulator , especially in the presence of additional hematopoietic cytokines . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Myeloid progenitors responsive to WEHI 3CM in combination with stem cell factor ( c kit ligand ) and B 220 B cell progenitors responsive to insulin like growth factor 1 in combination with interleukin 7 are not enriched . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Charcot Leyden crystal protein distribution in basophils and its absence in mast cells that differentiate from human umbilical cord blood precursor cells cultured in murine fibroblast culture supernatants or in recombinant human c kit ligand . ^^^ Suspension cultures of human umbilical cord blood mononuclear cells supplemented with c kit ligand containing additives give rise to a mixture of cells belonging to several lineages . ^^^ On the basis of findings reported here , the combined ultrastructural morphology and immunogold phenotyping of cells differentiating in c kit ligand supplemented cultures allows accurate lineage assignment of the developing cells . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Effects of interleukin 3 with or without the c kit ligand , stem cell factor , on the survival and cytoplasmic granule formation of mouse basophils and mast cells in vitro . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Growth in methylcellulose of human mast cells in hematopoietic colonies stimulated by steel factor , a c kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The c kit ligand , stem cell factor , promotes mast cell survival by suppressing apoptosis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Porcine stem cell factor / c kit ligand : its molecular cloning and localization within the uterus . ^^^ Stem cell factor ( SCF ) , or c kit ligand , is a multipotent growth factor that has been implicated in an important role in various aspects of animal development , including maintenance of the viability of primordial germ cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Steel ( SI ) and white spotting ( W ) loci encode steel factor ( c kit ligand ) and the c kit tyrosine kinase receptor , respectively . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Colony stimulating activity was provided by the recombinant human colony stimulating factors granulocyte macrophage colony stimulating factor ( GM CSF ) , granulocyte colony stimulating factor ( G CSF ) , interleukin 3 ( IL 3 ) , fusion protein ( FP ) , c kit ligand ( KL ) or GM CSF combined with other cytokines ( KL , IL 3 ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Effects of interleukin 3 and c kit ligand on the survival of various classes of human hematopoietic progenitor cells . ^^^ We examined the effects of interleukin 3 ( IL 3 ) and c kit ligand ( KL ) on the survival of differentiated hematopoietic progenitor cells ( HPC ) , the burst forming unit erythroid ( BFU E ) ; colony forming unit granulocyte , erythroid , monocyte , megakaryocyte ( CFU GEMM ) ; and CFU granulocyte monocyte ( CFU GM ) and more primitive hematopoietic cells that give rise to these progenitor cells ( pre colony forming cells [ pre CFC ] ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The purpose of the present study was to determine if the combination of tempol with stem cell factor ( SCF , c kit ligand ) would provide enhanced radiation protection in C 57 mice compared with the protection afforded by either agent alone . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In this report we demonstrate that murine bone marrow cells cultured in either interleukin ( IL ) 3 or mast cell growth factor ( MGF , also known as c kit ligand and stem cell factor ) differentially express cytokine genes . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Effects of recombinant human Steel factor ( c kit ligand ) on early cord blood hematopoietic precursors . ^^^ The purpose of this study was to define the effects of recombinant human Steel factor ( rhSF ) ( c kit ligand ) on early and late cord blood hematopoietic progenitors . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Rescue from anti MHC class 2 antibody mediated marrow graft failure by c kit ligand . ^^^ Administration of recombinant canine c kit ligand / stem cell factor ( SCF ) for 7 or 21 days posttransplant resulted in reversal of graft failure . ^^^ In conclusion , therefore , marrow graft failure induced by anti MHC class 2 MoAb does not appear to be epitope dependent , involves all hematopoietic lineages , and is overcome by the administration of c kit ligand . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mast cell growth factor ( c kit ligand ) restores growth of multipotent progenitors in myelodysplastic syndrome . ^^^ To determine if the c kit ligand mast cell growth factor ( MGF ) can improve progenitor growth in MDS , we evaluated in vitro responsiveness of bone marrow progenitors from 25 patients to MGF and / or GM CSF , interleukin 3 ( IL 3 ) and PIXY 321 , and examined the relationship between progenitor response and cellular expression of the c kit receptor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To test whether primitive hematopoietic stem cells ( PHSCs ) are stimulated by Steel ( SI ) factor ( c kit ligand ) in vivo , donor mice were studied after three or seven daily injections of SI factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The aim of this study was to analyze the potential of c kit ligand ( CKL ) combined with granulocyte macrophage colony stimulating factor ( GM CSF ) and / or interleukin 3 ( IL 3 ) to induce proliferation and differentiation of human bone marrow CD34+ cells in vitro . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The effects of recombinant canine granulocyte colony stimulating factor ( rcG CSF ) and recombinant canine stem cell factor ( rcSCF ) , a c kit ligand , on the circulation of hematopoietic progenitor and stem cells were studied in a canine model . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Other known mast cell chemoattractants including laminin , c kit ligand , and IL 3 were found to be considerably less potent on a molar basis in inducing directed migration . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The effect of soluble c kit ligand ( stem cell factor , SCF ) on human megakaryocytopoiesis of the cells from umbilical cord blood was evaluated in a methylcellulose culture containing human plasma . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Steel factor ( c kit ligand ) stimulates the in vitro growth of immature CD 3 / CD4 / CD8 thymocytes : synergy with IL 7 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The c kit ligand suppresses apoptosis of human natural killer cells through the upregulation of bcl 2 . ^^^ A subset of human natural killer ( NK ) cells ( CD 3 CD56bright ) are unique among lymphocytes in their constitutive expression of c kit , a tyrosine kinase cell surface receptor that binds c kit ligand ( KL ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Expression of c kit ligand in human keratinocytes . ^^^ The c kit ligand is expressed on tissue anchored stromal cells . ^^^ In the present study , we used the reverse transcriptase mediated polymerase chain reaction ( PCR ) technique to investigate whether human keratinocytes are able to express c kit ligand mRNA . ^^^ Two sets of primers were designed to distinguish two types of c kit ligand mRNA ( full length type and spliced type ) . ^^^ This result indicates the spontaneous transcription of full length type mRNA of the c kit ligand in human keratinocytes . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( c kit ligand ) enhances the interleukin 9 dependent proliferation of human CD34+ and CD34+CD33 DR cells . ^^^ We have studied the effects of recombinant human interleukin 9 ( IL 9 ) , alone and combined with stem cell factor ( SCF , c kit ligand ) , IL 3 , and granulocyte macrophage colony stimulating factor ( GM CSF ) on the clonogenic proliferation of highly enriched human hematopoietic CD34+ and CD34+CD33 DR progenitor cells . ^^^ Furthermore , IL 9 synergizes in vitro with the c kit ligand in expanding the pool of early pluripotent hematopoietic progenitor cells . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The human stem cell factor ( SCF ) , also termed c Kit ligand ( KL ) , is a hematopoietic growth factor produced by mesenchymal cells that induces proliferation of bone marrow progenitor cells , megakaryocytes , and mast cells via interaction with c Kit , its cognate receptor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mast cell growth factor ( C kit ligand ) in combination with granulocyte macrophage colony stimulating factor and interleukin 3 : in vivo hemopoietic effects in irradiated mice compared to in vitro effects . ^^^ In the presence of hemopoietic cytokines such as granulocyte macrophage colony stimulating factor ( GM CSF ) and interleukin 3 ( IL 3 ) , mast cell growth factor ( MGF ; also known as steel factor , stem cell factor , and c kit ligand ) has proven to be a potent hemopoietic regulator in vitro . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Pretreatment with epoxybutene in vitro suppressed recombinant interleukin 3 stimulated colony formation in haematopoietic stem and progenitor cells , had no effect on colony formation with recombinant granulocyte / macrophage colony stimulating factor or recombinant granulocyte colony stimulating factor alone , and completely blocked the synergism of recombinant c kit ligand and granulocyte / macrophage colony stimulating factor . ^^^ Butadiene induced leukaemogenesis , macrocytic anaemia and thymic atrophy are reminiscent of the conditions observed in mice bearing mutations at the W or Sl loci , which are deficient in the c kit receptor and c kit ligand , respectively . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Unexpectedly , in E 10 1 / 2 and 11 1 / 2d Wsh / Wsh embryos , we found c kit expression in the dermatome of the somites , the mesenchyme around the otic vesicle and the floorplate of the neural tube , structures known to express the c kit ligand in wild type embryos . ^^^ The ectopic c kit expression in Wsh homozygous embryos does not affect c kit ligand expression . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Cells were cultured in suspension in the presence of c kit ligand , interleukin 3 , interleukin 6 , erythropoietin , and granulocyte colony stimulating factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In 20 experiments , 1 10 10 ( 6 ) marrow or peripheral blood mononuclear cells ( PBMCs ) / mL were exposed to identical titers of pseudotyped PG13 / LN vector or PA317 / LN vector in the presence of recombinant human interleukin 1 ( IL 1 ) , IL 3 , IL 6 , and stem cell factor ( SCF ; c kit ligand ) for 5 days . 50 % of fresh vector supernatant was refed daily . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Expression of the c kit ligand and interleukin 6 genes in mouse bone marrow stromal cell lines . ^^^ The expression of c kit ligand and interleukin 6 ( IL 6 ) genes in mouse bone marrow derived stromal cell lines was examined using quantitative polymerase chain reaction ( PCR ) analysis based on the design of an internal DNA control . ^^^ All the cell lines expressed c kit ligand gene constitutively , and this expression was not increased by lectins . ^^^ The constitutive expression of c kit ligand in 14F1 . 1 cells was the lowest among the three cell lines studied and could be increased by stimulation with IL 4 . ^^^ However , the unique capacity of 14F1 . 1 cells to support in vitro hemopoiesis can not thus far be explained solely on the basis of the ability of these cells to secrete cytokines which are not produced by other stromal cell lines . c kit ligand may be necessary , but its presence alone is not sufficient for 14F1 . 1 cells to support prolonged hemopoiesis . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
C kit ligand induction of immature neutrophils in cultures of human umbilical cord blood . ^^^ Human cord blood cells cultured in suspension with soluble c kit ligand produce immature mast cells from their agranular precursors ; cocultures of cord blood and mouse 3T3 fibroblasts produce fully mature human mast cells . ^^^ We noted cells of the neutrophil lineage in the c kit ligand supplemented suspension cultures . ^^^ Similar cultures were prepared from individual cord bloods with several sources of the c kit ligand , including mouse fibroblast conditioned media , a partially purified mouse 3T3 fibroblast factor ( s ) , recombinant human stem cell factor , recombinant murine mast cell growth factor , and were sampled sequentially for routine and cytochemical ultrastructural studies . ^^^ These studies show that peroxidase positive azurophilic granule containing neutrophilic myelocytes develop in quantity from their agranular precursors in cord blood when the c kit ligand is present , but little to no maturation to mature neutrophils with specific granules occurs . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
A study of gene expression of GM CSF , IL 3 , c kit ligand , and IL 1 by bone marrow stromal cells of mice who were treated with an LD 50 cytosine arabinoside ( Ara C ) dose was carried out . ^^^ The c kit ligand gene was expressed by layers during marrow regeneration and by control layers , but was absent during the stage of hematopoietic damage . ^^^ We conclude that GM CSF , c kit ligand and IL 1 have important roles in sustaining marrow regeneration following extensive damage . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Major histocompatibility complex class 2 mediated inhibition of hemopoiesis in vitro and in vivo is abrogated by c kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , the product of the steel gene in mice ( also termed mast cell growth factor , c kit ligand , or Steel factor ) , is a hematopoietic growth factor demonstrating substantial synergistic activity with a number of other cytokines on primitive hematopoietic stem and progenitor cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Melanocyte precursors transiently require the c kit ligand , Steel factor for survival . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Effect of stem cell factor ( c kit ligand ) on clonogenic leukemic precursor cells : synergy with other hematopoietic growth factors . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( CSF ) , also called c kit ligand ( KL ) or mast cell growth factor ( MGF ) is a peptide growth factor / cytokine with broad activities , especially on hematopoiesis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In vitro studies of the CD34+ Lin population have indicated that the blast sized cells , which are presumably in cycle , proliferate and give rise to colony forming cells in the presence of combinations of growth factors , including c kit ligand and interleukin 3 ( IL 3 ) . ^^^ These studies have shown that an interaction with marrow stromal cells is required , in addition to c kit ligand and IL 3 , to induce these cells to proliferate and form multiple colony forming cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stromal cell lines derived from canine long term bone marrow cultures ( LTBMC ) were characterized regarding the expression of growth factors and especially the localization of stem cell factor ( SCF ) ( c kit ligand ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Synergy among erythropoietin , interleukin 3 , stem cell factor ( c kit ligand ) and interferon gamma on early human hematopoiesis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The number of progenitor cells from transduced CD 34 positive cells increased 50 fold after cytokine prestimulation with interleukin 1 ( IL 1 ) , c kit ligand ( KL ; stem cell factor ) , and IL 3 and 2 weeks in liquid culture . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Expression and functional role of c kit ligand ( SCF ) in human multiple myeloma cells . ^^^ The c kit ligand also enhanced the proliferation of MT 3 and U 266 cells mediated by the other cytokines . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Current models suggest that early acting MK colony stimulating factors , such as interleukin ( IL ) 3 or c kit ligand , are required for expansion of hematopoietic progenitors into cells capable of responding to late acting MK potentiators , including IL 6 and IL 11 . ^^^ In this report we demonstrate that neutralizing the biological activity of Tpo eliminates MK formation in response to c kit ligand , IL 6 , and IL 11 , alone and in combination , but that these reagents only partially reduce MK formation in the presence of combinations of cytokines including IL 3 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Retinoic acid upregulates c kit ligand production by murine keratinocyte in vitro and increases cutaneous mast cell in vivo . ^^^ Anti c kit ligand antibody abrogated MC 9 GF activity and RT PCR analysis demonstrated that retinoic acid upregulates c kit ligand mRNA expression by Pam cells . ^^^ The presence of anti IL 1 alpha , IL 1 beta , IL 2 , IL 3 or IL 4 antibodies did not abrogate the MC 9 GF activity except for anti c kit ligand antibody . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The c kit ligand ( KL ; Steel factor , mast cell growth factor , stem cell factor ) is a hematopoietic factor that has been shown to act as a potent cofactor for hematopoietic growth and differentiation in vitro . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Cell cell interactions and the presence of growth factors such as stem cell factor ( SCF ; or c kit ligand ) or interleukin 6 ( IL 6 ) are involved in the proliferation and differentiation of the canine marrow derived stromal cell line DO 64 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , a c kit ligand , has a preferential effect on the proliferation of several classes of immature hematopoietic progenitor cells in combination with GM CSF or IL 3 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In contrast , common erythroid progenitors ( termed SCF progenitors ) express only c Kit and undergo a limited number of cell divisions in response to the c Kit ligand , stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To establish whether recombinant human growth factors are beneficial during transduction , the presence of interleukin 3 ( IL 3 ) , IL 6 , and mast cell growth factor ( MGF , a c kit ligand ) were compared in different combinations . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Expansion of immunostimulatory dendritic cells among the myeloid progeny of human CD34+ bone marrow precursors cultured with c kit ligand , granulocyte macrophage colony stimulating factor , and TNF alpha . ^^^ These bone marrow CD34+ derived dendritic cell that develop during 2 wk culture have the same morphologic , phenotypic , and functional properties that distinguish mature dendritic cells in blood . c kit ligand does not directly influence dendritic cell differentiation per se , but rather increases the total cell number in synergistic combination with GM CSF and TNF alpha . ^^^ The synergistic combination of c kit ligand , GM CSF , and TNF alpha therefore expands substantial numbers of immunostimulatory CD 14 HLA DR+ dendritic cells from defined CD34+ progenitors in human bone marrow . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
SPY 3 2 expressed high levels of c kit ligand and low levels of granulocyte macrophage colony stimulating factor and interleukin 3 , and these molecules were all involved in this long term erythropoiesis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The immediate phase of c kit ligand stimulation of mouse bone marrow derived mast cells elicits rapid leukotriene C 4 generation through posttranslational activation of cytosolic phospholipase A 2 and 5 lipoxygenase . c kit ligand ( KL ) activated mouse bone marrow derived mast cells ( BMMC ) for the dose and time dependent release of arachidonic acid from cell membrane phospholipids , with generation of leukotriene ( LT ) C 4 in preference to prostaglandin ( PG ) D 2 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
While both c kit ligand ( KL ) and IL 7 are the requisite molecules for sustained proliferation of B precursor cells , each molecule plays distinct roles . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Steel factor ( c kit ligand ) promotes the survival of hematopoietic stem / progenitor cells in the absence of cell division . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We assessed the effects of the c kit ligand , stem cell factor ( SCF ) , in the jejunal mucosal mast cell hyperplasia that occurs during infection with the intestinal nematodes , Nippostrongylus brasiliensis or Trichinella spiralis in rats . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To determine the potential role of autocrine growth factor production in regulating primitive human hematopoietic cell development , we examined highly purified CD34+ , c Kit+ marrow mononuclear cells for expression of c Kit ligand ( KL ) and stem cell tyrosine kinase 1 ( stk 1 ) ligand ( STK 1 L ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Expression of c kit ligand messenger ribonucleic acids in human ovaries and regulation of their steady state levels by gonadotropins in cultured granulosa luteal cells . ^^^ The c kit ligand ( KL ) , a ligand for the c kit protooncogene receptor tyrosine kinase , is an important regulator of germ cell development in rodent gonads . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In this study , hematopoietic progenitor cells ( HPCs ) , stringently purified from peripheral blood , were induced to megakaryocytic differentiation / maturation in serum free liquid suspension culture treated with a growth factor cocktail ( interleukin 3 [ IL 3 ] , c kit ligand , and IL 6 ) and / or recombinant mpl ligand ( mpIL ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Although IL 12 has been reported to synergize with c kit ligand ( KL ) in promoting hematopoietic stem cell proliferation in vitro , administration of recombinant mouse IL 12 ( rIL 12 ) to normal mice caused a dose and time dependent anemia , leukopenia , and thrombocytopenia in vivo . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Follicle stimulating hormone ( FSH ) and its intracellular mediator , cAMP , increase the mRNA levels for the Steel factor ( SLF , the c kit ligand ) in cultured primary mouse Sertoli cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Effects of recombinant canine stem cell factor , a c kit ligand , and recombinant granulocyte colony stimulating factor on hematopoietic recovery after otherwise lethal total body irradiation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Interleukin 4 suppresses c kit ligand induced expression of cytosolic phospholipase A 2 and prostaglandin endoperoxide synthase 2 and their roles in separate pathways of eicosanoid synthesis in mouse bone marrow derived mast cells . ^^^ Mouse bone marrow derived mast cells ( BMMCs ) developed with interleukin 3 ( IL 3 ) can be stimulated by c kit ligand ( KL ) and accessory cytokines over a period of hours for direct delayed prostaglandin ( PG ) generation or over a period of days to prime for augmented IgE dependent PG and leukotriene ( LT ) production , as previously reported . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Characterization of lineage negative blast subpopulations derived from normal and chronic myelogenous leukemia bone marrows and determination of their responsiveness to human c kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The ability of CD34+ / c kit+ cells to form multilineage colonies ( CFU GEMM ) was assayed after sorting with an antibody that did not show any significant effect on c kit ligand ( RL ) or granulocyte / macrophage colony stimulating factor ( GM CSF ) induced colony formation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We examined whether three cytokines that promote mouse mast cell development , the c kit ligand stem cell factor ( SCF ) , IL 3 , or IL 4 , also can directly stimulate or modulate mouse peritoneal mast cell ( PMC ) mediator release . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Development of human mast cells from umbilical cord blood cells by recombinant human and murine c kit ligand . ^^^ Both human and mouse c kit ligand induced differentiation of human mast cells in a long term culture of the mononuclear cells of umbilical cord blood . ^^^ Growth factor activity for human mast cells present in conditioned medium of BALB / 3T3 fibroblasts was due to mouse c kit ligand . ^^^ Recombinant c kit ligand induced differentiation and proliferation of mast cell progenitors in early stages of culture . ^^^ However , apparent selective growth of mast cells by c kit ligand in cord blood cell cultures is mainly due to the effect of the cytokine to selectively maintain survival of immature mast cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In clonogenetic dishes , graded amounts of c kit ligand ( KL ) were added together with saturating levels of erythropoietin ( Ep ) and variable amounts of interleukin 3 and granulocyte macrophage colony stimulating factor ( IL 3 / GM CSF ) , ie , high or low level , or no IL 3 / GM CSF addition . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Cell line stimulation and antibody blocking indicated the presence of c kit ligand , interleukin 6 ( IL 6 ) and granulocyte colony stimulating factor ( G CSF ) . ^^^ Baseline or `` constitutive ' ' expression of BMM and mRNA for CSF 1 and c kit ligand was seen . ^^^ There was a biphasic response of c kit ligand mRNA expression to CSF 1 exposure ; low levels of CSF 1 ( 50 U / mL ) induced , while higher levels ( 2000 U / mL ) inhibited , expression . ^^^ These data indicate that BMM ( and by analogy the macrophage component of Dexter culture stroma ) , are important sources of CSF 1 and c kit ligand but not GM CSF or IL 3 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Human stem cell factor ( c kit ligand ) induces an autocrine loop of growth in a GM CSF dependent megakaryocytic leukemia cell line . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Colony formation was assessed in 1 degree wells containing various cytokines , alone and in combination , and single colonies deriving after 21 days in semisolid medium were replated into 2 degree wells in the presence of the combination of purified preparations of recombinant human steel factor ( SF , a c kit ligand ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , granulocyte colony stimulating factor ( G CSF ) , interleukin 3 ( IL 3 ) , and erythropoietin ( Epo ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Steel factor ( SLF , c kit ligand ) , a potent costimulating cytokine in vitro for myeloid progenitor cells from normal donors , is currently being evaluated in clinical trials for effects on hematopoiesis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
This is reminiscent of pathologies observed in mice bearing mutations at the W and Sl loci , which are deficient in c kit and c kit ligand ( CKL ) , respectively . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Expansion of hemopoietic activity in long term culture of human bone marrow by c kit ligand ( stem cell factor ) . ^^^ The response of hemopoietic activity in long term culture of human bone marrow ( LTMC ) to exogenous c kit ligand has been investigated . ^^^ Addition of c kit ligand as recombinant Stem Cell Factor after the adherent stromal cell layer had become confluent failed to increase the content of hemopoietic cells in the culture system . ^^^ Production of hemopoietic progenitors and differentiated progeny was on the other hand amplified by addition of c kit ligand at regular intervals from the initiation of the culture , and resulted in net formation of these cells in the culture system . ^^^ The characteristics of the response were consistent with consequences of increased survival of hemopoietic progenitors during a vulnerable initial phase in which the availability of endogenous c kit ligand was limited by the initially low number of stromal cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Altered metabolism of mast cell growth factor ( c kit ligand ) in cutaneous mastocytosis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Binding of germ cells to mutant Sld Sertoli cells is defective and is rescued by expression of the transmembrane form of the c kit ligand . ^^^ Mutations in the steel ( Sl ) locus , encoding the c kit ligand ( KL ) , are associated with impaired germ cell development in mice . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The expression pattern of the c kit ligand in gonads of mice supports a role for the c kit receptor in oocyte growth and in proliferation of spermatogonia . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
When WJic / WJic , Wn / Wn , and Wf / Wf CMCs were stimulated by the recombinant c kit ligand , autophosphorylation activity was observed only in Wf / Wf CMCs . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To that effect , stromal cell free LTBMC were initiated with CD34+ HLA DR CD 15 rhodamine 123dull bone marrow cells and were maintained with repeated addition of c kit ligand and a synthetic interleukin 3 / granulocyte macrophage colony stimulating factor fusion protein . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Synergism between serum factor ( s ) and erythropoietin in inducing murine megakaryocyte colony formation : the synergistic factor in serum is distinct from interleukin 11 and stem cell factor ( c kit ligand ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The in vivo role of stem cell factor ( c kit ligand ) on mastocytosis and host protective immunity to the intestinal nematode Trichinella spiralis in mice . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Other basophil primers for anti IgE dependent histamine release were IL 5 , mast cell growth factor ( c kit ligand ) , and insulin like growth factor 2 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Four cytokine combinations were tested : ( 1 ) mast cell growth factor ( MGF ; a c kit ligand ) and interleukin 6 ( IL 6 ) ; ( 2 ) MGF , IL 6 , IL 3 , and erythropoietin ( Epo ) ; ( 3 ) MGF , IL 6 , granulocyte macrophage colony stimulating factor ( GM CSF ) / IL 3 fusion protein ( FP ) , macrophage colony stimulating factor ( M CSF ) , and granulocyte CSF ( G CSF ) ; and ( 4 ) MGF , IL 6 , FP , M CSF , G CSF , and Epo . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Co stimulatory effects of steel factor , the c kit ligand , on purified human hematopoietic progenitors in low cell density culture . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
CD34+ cells enriched from bone marrow were cultured for up to 5 weeks in interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) and granulocyte colony stimulating factor ( G CSF ) with or without stem cell factor ( SCF ) ( also termed c kit ligand ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Since the c kit ligand is a product of murine fibroblasts , we examined the growth effect of recombinant human c kit ligand ( stem cell factor ) , of recombinant murine c kit ligand ( mast cell growth factor ) , and of a partially purified fraction derived from mouse fibroblast culture supernatant on the mast cell lineage of humans by electron microscopy in 8 week cultures of cord blood cells . ^^^ We found that immature mast cells which developed in cultures containing the recombinant ligand for c kit of human or murine origin as well as the naturally occurring c kit ligand in 3T3 fibroblast supernatants were identical . ^^^ Thus , each of these sources of the c kit ligand exerted identical effects on the ontogeny of human mast cells as they develop from their agranular precursors in cord blood . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
SLF , the c Kit ligand which is encoded by the Sl locus , has growth promoting activity for hemopoietic stem cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To investigate how c kit and c kit ligand play a role in the function of hematopoietic stem cells , we determined the expression of c kit in sorted human hematopoietic stem cells , CD34+CD33 cells and CD34+CD33+ cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
A relatively frequent progenitor expressing only c Kit transiently proliferated in response to avian c Kit ligand ( stem cell factor [ SCF ] ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Recombinant human stem cell factor , a c kit ligand , stimulates granulopoiesis in severe congenital neutropenia . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Finally , we show that T lymphopoiesis was enhanced by c kit ligand . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Association of hematopoietic cell phosphatase with c Kit after stimulation with c Kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Paracrine and autocrine growth mechanisms of human stem cell factor ( c kit ligand ) in myeloid leukemia . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We previously described that cells with a CD34+CD71lo phenotype from adult human bone marrow are maintained at constant numbers in long term suspension cultures supplemented with interleukin 6 ( IL 6 ) , IL 3 , mast growth factor ( MGF ) ( a c kit ligand ) , and erythropoietin ( Epo ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Differences in the composition and in the efficiency of red cell production of normal and CML erythroid progenitor populations are highlighted by response to human c kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Prevention of apoptosis by c kit ligand . ^^^ It is well established that mast cell proliferation and maturation are regulated by two principle cytokines , IL 3 and the c kit ligand stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Recent data suggest that stem cell factor ( SCF or c kit ligand , KL ) is a major regulator of human mast cells ( MCs ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In both murine and human systems the c kit ligand , also known as mast cell growth factor ( MGF ) , acts synergistically with several colony stimulating factors , including the granulocyte macrophage colony stimulating factor ( GM CSF ) and interleukin 3 ( IL 3 ) , in stimulating the proliferation and differentiation of different types of hematopoietic progenitors . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The c kit ligand or stem cell factor ( SCF ) and the c kit ligand receptor ( KR ) are thought to play pivotal roles in the regulation of human hematopoiesis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Effects of chronic treatment with the c kit ligand , stem cell factor , on immunoglobulin E dependent anaphylaxis in mice . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Binding of c kit ligand , stem cell factor ( SCF ) to c kit receptor ( c kitR ) is known to activate c kitR tyrosine kinase , thereby leading to autophosphorylation of c kitR on tyrosine and to association of c kitR with substrates such as phosphatidylinositol 3 kinase ( PI3K ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
These mast cells can be induced by recombinant c kit ligand ( rKL ) to obtain high steady state levels of the mMCP 4 transcript and by rIL 10 to obtain high steady state levels of the mMCP 1 and mMCP 2 transcripts . rIL 3 and rKL both elicit the differentiation of progenitor cells into immature BMMC and then stimulate their proliferation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Effect of c kit ligand ( stem cell factor ) in combination with interleukin 5 , granulocyte macrophage colony stimulating factor , and interleukin 3 , on eosinophil lineage . ^^^ The effects of recombinant human stem cell factor ( SCF , a c kit ligand ) on an eosinophil lineage were examined in clonal and suspension cultures of human non adherent light density bone marrow cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Although the potentiating effect of stromal cells on pro B cell proliferation can be partially attributed to the ligand for the proto oncogene receptor c kit ( c kit ligand [ KL ] or stem cell factor ) , several lines of evidence suggest that c kit mediated cell signalling is not required for pro B cell expansion . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Ultrastructural morphology of immature mast cells in sequential suspension cultures of human cord blood cells supplemented with c kit ligand ; distinction from mature basophilic leukocytes undergoing secretion in the same cultures . ^^^ The ability to culture human mast cells and , thus , to determine their ontogeny and possible relationships to other lineages has been facilitated by new studies using cocultures of cord blood cells with mouse fibroblasts and recombinant human or murine c kit ligand supplemented suspension cultures of cord blood cells . ^^^ In this study , we examined c kit ligand supplemented cord blood cell suspension cultures designed so that the effects of growth factor source , individual cord sample , and culture time ( 3 17 weeks ) on the developing mast cell lineage could be individually evaluated . ^^^ We conclude that fibroblasts are necessary and sufficient for the differentiation and maturation of human mast cells in vitro from their agranular precursors in cord blood but that soluble c kit ligand is not . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Effect of stem cell factor ( c kit ligand ) , granulocyte macrophage colony stimulating factor and interleukin 3 on hematopoietic progenitors in human long term bone marrow cultures . ^^^ We evaluate the effects of recombinant human stem cell factor ( SCF or c kit ligand ) alone and also in combination with recombinant human granulocyte macrophage colony stimulating factor ( GM CSF ) and interleukin 3 ( IL 3 ) , on cell proliferation and differentiation in human long term bone marrow cultures ( LTBMC ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We have studied the effects of recombinant human interleukin 11 ( rhIL 11 ) , alone and combined with stem cell factor ( SCF or c kit ligand ) , IL 3 , and granulocyte macrophage colony stimulating factor ( GM CSF ) on the proliferation of highly enriched human hematopoietic CD34+ and CD34+CD33 DR progenitor cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Although many proteins are thought to support the expansion of megakaryocytic precursor cells ( e . g . , interleukin [ IL ] 3 , c kit ligand [ KL ] ) , identification of the late acting , lineage specific growth factor for platelet production , termed Thrombopoietin ( Tpo ) , has remained elusive . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Low numbers of day 9 EB cells ( 5 10 10 ( 5 ) or less ) cultured in the presence of several growth factors ( interleukin 3 [ IL 3 ] , IL 1 , c kit ligand , basic fibroblast growth factor , insulin growth factor 1 , IL 6 , granulocyte colony stimulating factor , fetal liver kinase 2 ligand ) develop few or no CFCs after 1 week of culture . ^^^ When these cells are plated on irradiated NIH 3T3 with IL 3 or c kit ligand or combinations containing these and other growth factors , they are able to generate CFCs for at least 3 weeks . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Expansion of marrow in all cases was accomplished through the use of added cytokines such as IL 3 , GM CSF , and c kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
When mouse bone marrow derived mast cells developed in interleukin 3 ( IL 3 ) containing medium were cultured with c kit ligand in combination with IL 10 and IL 1 beta , transient expression of PGHS 2 mRNA and protein occurred in a dose and time dependent fashion , accompanied by substantial release of prostaglandin D 2 ( PGD 2 ) into the culture medium from 2 to 10 h . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Also presented are eosinophil morphologies that occur in vitro in suspension cultures of human cord blood supplemented with the c kit ligand from various sources . ^^^ The wide variety of eosinophil subcellular changes in the c kit ligand supplemented cultures , like the changes of which eosinophils are capable in vivo , reflects the processes of differentiation , maturation , activation , secretion and cell injury . ^^^ Presentation of this ultrastructural morphological primer of human eosinophils in vitro should enable investigators to recognize eosinophils in all of their diverse morphologic forms in cultures that contain differentiating and functioning members of other lineages , also present in c kit ligand supplemented cultures . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
HSNC clones produced various cytokines , including IL 6 , IL 7 , IL 8 , IL 9 , granulocyte CSF ( G CSF ) , granulocyte macrophage CSF ( GM CSF ) , macrophage CSF ( CSF 1 ) , TGF beta 1 , and c kit ligand , but could not produce IL 1 alpha , IL 1 beta , IL 2 , IL 3 , IL 4 , TNF alpha , or TNF beta . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The development of fully mature mast cells occurred regularly in fibroblast containing cocultures ; partially mature mast cells developed in fibroblast culture supernatant , partially purified mouse fibroblast factor ( s ) , and either recombinant human or murine c kit ligand supplemented suspension cultures . ^^^ Small numbers of basophils and eosinophils were present in the suspension cultures that received c kit ligand in its recombinant or naturally occurring forms . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
They were cocultured for 4 days with fibroblasts producing a high titer of retrovirus in medium containing combinations of the hematopoietic growth factors interleukin 3 ( IL 3 ) , IL 6 , c kit ligand ( KL ) , and leukemia inhibitory factor ( LIF ) and then injected into lethally irradiated recipients , together with sufficient `` compromised ' ' bone marrow cells to provide short term support . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Particularly the identification of new surface membrane molecules such as CD 40 ligand on basophils or c kit on mast cells , and of new triggering agents and modulators of mediator release such as IL 3 , IL 5 , GM CSF and nerve growth factor ( for basophils ) or c kit ligand ( for mast cells ) allows a better understanding of the regulation of these cell types . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In contrast , no mast cell protease transcripts are present in abundance in the ear and skin of WBB6F1 / J W / Wv ( W / Wv ) and WCB6F1 / J Sl / Sld ( Sl / Sld ) mice which are mast cell deficient in vivo due to defects in their c kit and c kit ligand genes , respectively . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Purified HPCs induced into cycling by growth factors ( interleukin 3 , interleukin 6 , c kit ligand ) were transduced with the N 2 retroviral vector containing the neomycin resistance ( neor ) gene . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We show that the GM CSF plus TNF alpha dependent ex vivo generation of DCs from mobilized CD34+ cells is 2 . 5 fold enhanced by flk 2 / flt 3 ligand or c kit ligand ( stem cell factor ) and five fold enhanced by a combination of these growth factors . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To investigate whether cell losses following T cell depletion could be overcome by ex vivo expansion , cells were cultured in the presence of recombinant human interleukin 3 ( rhIL 3 ) and recombinant human c kit ligand ( stem cell factor [ rhSCF ] ) for 7 days . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Methylcellulose committed progenitor cell assays were carried out using FL alone and in combinations with granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) and c kit ligand ( KL ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
These results indicate that CPA is a novel factor distinct from IL 1 , IL 3 , G , M , GM CSF , IL 6 and SCF ( c kit ligand ) . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The results suggest that c kit ligand ( KL ) plays a key role in the reactivation of HbF synthesis in adult life , and IL 3 / GM CSF potentiate this effect at low KL level . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The major growth factor for mast cell development is c kit ligand , which induces mastocytosis in vivo . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In clonogenetic fetal calf serum supplemented ( FCS+ ) or nonsupplemented ( FCS ) culture treated with saturating levels of interleukin 3 ( IL 3 ) granulocyte macrophage colony stimulating factor ( GM CSF ) and erythropoietin ( Ep ) ( combined with c kit ligand in FCS ( ) culture conditions ) , RA induces a dramatic dose dependent shift from erythroid to granulomonocytic colony formation , the latter colonies being essentially represented by granulocytic clones . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The factors regulating this release may include histamine releasing factors , interleukin 3 , the c kit ligand , the cyclic nucleotide system , psychological factors , metabolites of arachidonic acid and cytokines . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Trophoblastic receptors for the c kit ligand ( stem cell factor ) could not be identified using the same methods . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
So far identified are two membrane receptor tyrosine kinases , c Kit and PDGF R / alpha , and the ligand for c kit , MGF ( mast cell growth factor , also known as stem cell factor , c Kit ligand , or steel factor ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
CD34+ / Thy 1+ / Lin cells from adult BM or MPB were labeled with the fluorescent membrane dye PKH 26 , followed by in vitro culture of 10 ( 4 ) cells on a murine stromal layer in the presence of interleukin ( IL ) 3 , IL 6 , c kit ligand ( KL ) , and leukemia inhibitory factor ( LIF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Effect of c kit ligand , stem cell factor , on mediator release by human intestinal mast cells isolated from patients with inflammatory bowel disease and controls . ^^^ This study examined the effect of two cytokines ( c kit ligand and interleukin 3 ) and other agonists on human intestinal mast cell function . ^^^ After preincubation without or with c kit ligand or interleukin 3 , cells were stimulated by IgE receptor crosslinking , C5a or formyl methionyl leucyl phenylalanine ( fMLP ) . ^^^ The sequential stimulation of the cells with c kit ligand and IgE receptor crosslinking induced the release of high amounts of histamine and leukotrienes , whereas each agonist by itself induced only marginal mediator release . ^^^ No significant mediator release was seen in response to C5a and fMLP , even if the cells were pretreated with c kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In contrast , both cell lines expressed c kit ligand mRNA only in the absence of L ascorbate and beta glycerol phosphate induction . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In previous studies using stroma free short term assays , Flt 3 ligand ( FL ) has been shown to induce proliferation of HPC at proportions similar to or less than c kit ligand ( steel factor , SF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To further address the role of IL 9 in the development of acute leukemia , we evaluated the proliferative response of three leukemic cell lines and 32 primary samples from acute myeloblastic leukemia ( AML ) patients to recombinant human ( rh ) IL 9 alone and combined with rh IL 3 , granulocyte macrophage CSF ( GM CSF ) , and stem cell factor ( [ SCF ] c kit ligand ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( c kit ligand ) influences eosinophil recruitment and histamine levels in allergic airway inflammation . ^^^ A primary inducer of mast cell differentiation , proliferation , and activation has been identified as c kit ligand or stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , also termed mast cell growth factor or c kit ligand , plays a central role in the regulation of hematopoiesis and maintenance of viability of hematopoietic cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The role of stem cell factor ( c kit ligand ) and inflammatory cytokines in pulmonary mast cell activation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
A number of studies have shown that human CD 3 CD56+ NK cells can be obtained from BM derived CD34+ hematopoietic progenitor cells ( HPCs ) cultured in the presence of interleukin 2 ( IL 2 ) and an allogeneic feeder cell layer , or IL 2 and other hematopoietic growth factors such as the c kit ligand ( KL ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Using a plasma clot clonal assay and a long term bone marrow culture ( LTBMC ) system , we studied the effects of the recently cloned human flt 3 ligand ( FL ) alone and in combination with granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) , or stem cell factor ( c kit ligand [ KL ] ) on human megakaryocytopoiesis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In contrast , cytokines such as IL 1 , IL 2 , IL 3 , IL 4 , IL 6 , G CSF , GM CSF , or SCF ( c kit ligand ) altered neither the proliferation capacity nor the phenotypical constellation of LW / SO cells ( each tested alone ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mast cells expressing chymase but not tryptase can be derived by culturing human progenitors in conditioned medium obtained from a human mastocytosis cell strain with c kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In analyzing proteins tyrosine phosphorylated in primary primitive lin normal blasts in response to various hematopoietic growth factors , we found a striking similarity in the phosphorylation of four major ( approximately 140 , 110 , 62 and 56 kDa ) and three minor ( approximately 51 , 45 and 42 kDa ) P tyr proteins after stimulation with c kit ligand and the P tyr proteins constitutively phosphorylated in primary primitive lin chronic phase CML blasts . ^^^ It is provocative that at least seven proteins rapidly and transiently phosphorylated on tyrosine in the c kit ligand signal transduction pathway in lin normal blasts may be constitutive substrates for the p 210 activated tyrosine kinase in comparable lin chronic phase CML blasts . ^^^ In addition , it is intriguing that some of the biological effects on hematopoietic progenitors attributed to the c kit ligand may be similar to some of the observed biological consequences of the p 210 protein , including survival and expansion of a more mature stem cell population , probably at the time of lineage commitment rather than at the level of the earliest self renewing stem cell . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Thus we have found little evidence of over expression of c kit in adult AML . mRNA for the c kit ligand , Steel Factor ( SLF ) was also quantitated by RPA in these specimens . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
CD34+ precursors in normal human bone marrow ( BM ) generate large numbers of dendritic cells alongside macrophages and granulocytic precursors when cultured for 12 to 14 days in c kit ligand , granulocyte macrophage colony stimulating factor ( GM CSF ) , and tumor necrosis factor alpha ( TNF alpha ) . ^^^ Therefore , normal CD34+ BM precursors can generate a post CFU bipotential intermediate in the presence of c kit ligand , GM CSF , and TNF alpha . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The priming effect on epinephrine induced platelet aggregation in hirudin PRP was unique to thrombopoietin , with no effects seen using interleukin 6 ( IL 6 ) , IL 11 , IL 3 , erythropoietin , granulocyte colony stimulating factor , granulocyte macrophage colony stimulating factor , or c kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Bone marrow cells were enriched for CD34+ cells and then placed in liquid cultures supplemented with either interleukin 3 ( IL 3 ) or IL 3 and c kit ligand with and without the addition of IGF 2 . ^^^ IGF 2 also promoted an increase in cellularity in cultures with IL 3 and c kit ligand . ^^^ In combination with IL 3 or IL 3 and c kit ligand , IGF 2 promoted an earlier differentiation of granulocytes , as well as an increase in the number of megakaryocyte lineage cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Cbl protein in human hematopoietic cells . c kit ligand ( KL ) is a hematopoietic growth factor that plays a major role in the survival , expansion and differentiation of hematopoietic progenitor cells of various lineages . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Isolated CD34+lin+ cells formed increased numbers of colony forming cells ( CFC ) when flt 3l was added together with IL 3 , IL 6 , G CSF , GM CSF or c kit ligand ( KL ) , or with the combination of IL 3 and KL . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
IL 11 is synergistic with stem cell factor ( c kit ligand ) in vitro , enhancing proliferation of primitive hematopoietic stem cells , and has been shown to be critical to the process of polyploidization and maturation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In contrast , when recombinant c kit ligand ( rKL ) was added to rGM CSF and rEPO , an increment in colony numbers was observed that was eliminated by rIP 10 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Further , the DNA synthesis stimulated by the c Kit ligand stem cell factor at a concentration of 1 nM was synergistically enhanced by 5 nM ET 1 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We also used the progenitor assay to screen several human and murine recombinant cytokines for cross reactivity to swine bone marrow cells , including interleukin 3 ( IL 3 ) , IL 6 , Il 11 , granulocyte and granulocyte macrophage colony stimulating factors ( G CSF and GM CSF ) , c kit ligand ( also called mast cell growth factor [ MGF ] ) , and erythropoietin ( Epo ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The biological effects of c kit ligand ( stem cell factor : SCF ) on an immortalized human megakaryocytic cell line ( CMK ) was evaluated using methods including the 3 ( 4 , 5 dimethylthiazol 2 yl ) 2 , 5 diphenyltetrazolium bromide ( MTT ) assay , surface marker analysis , DNA cell cycle analysis and immunoblotting . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Post transcriptional stabilization by interleukin 1beta of interleukin 6 mRNA induced by c kit ligand and interleukin 10 in mouse bone marrow derived mast cells . ^^^ The addition of c kit ligand ( KL ) and IL 10 to IL 3 derived mouse bone marrow mast cells ( BMMC ) elicited an approximately 2 fold increase in steady state IL 6 mRNA levels that peaked after 0 . 5 h and was followed by the release of approximately 0 . 2 ng of IL 6 / 10 ( 6 ) cells by 5 7 h . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Both IGF 1 and c kit ligand ( SCF ) cause an additive increase in c myc mRNA in human CFU E in conjunction with EPO . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
C kit ligand ( SCF ) in human multiple myeloma cells . ^^^ The c kit ligand also enhanced the proliferation of MT 3 and U 266 cells mediated by the other cytokines . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Cloning , sequencing and expression of stem cell factor ( c kit ligand ) cDNA of brushtail possum ( Trichosurus vulpecula ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Topical tretinoin increases dermal mast cells , induces epidermal mast cell growth factor ( c kit ligand ) and modulates its distribution in hairless mice . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Human c kit ligand ( stem cell factor ) induces platelet Fc receptor expression in megakaryoblastic cells . ^^^ In an effort to establish a cellular model for induction of Fc gamma RIIA expression during megakaryocyte development by hematopoietic growth factors , steady state Fc gamma RIIA mRNA levels were monitored in c kit receptor positive megakaryocytic cells ( M07e , HEL , and Dami ) in response to c kit ligand ( KL ; also known as stem cell factor , mast cell growth factor , or Steel factor ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To identify the earliest human hematopoietic cells on which TPO acts , we cultured single CD34+Thy 1+Lin adult bone marrow cells in the presence of TPO alone , with TPO and interleukin 3 ( IL 3 ) , or with TPO and c kit ligand ( KL ) in the presence of a murine stromal cell line ( Sys 1 ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Continuous culture of these hematopoietic cells in the presence of the cytokines interleukin 3 , c kit ligand , granulocyte colony stimulating factor resulted in tenfold expansion by day 7 and 25 fold expansion by day 14 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
A subculture of this line can be maintained in recombinant human c kit ligand [ stem cell factor ( SCF ) ] at 100 ng / ml without requirement for other growth factors . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Thus , 1 ) a coordinate activation of HOXB genes from the 3 ' > 5 ' cluster side apparently underlies IL 2 / IL 1 beta induced NK cell activation . 2 ) Since NK cell activation and survival induced by IL 12 and c kit ligand , respectively , are not associated with cell proliferation of HOXB gene expression , it is apparent that HOXB gene induction is specifically associated with IL 2 induced NK cell proliferation . 3 ) Studies with antisense oligomer targeting HOXB 2 mRNA suggest an important role for 82 in NK cell proliferation , possibly in part via the IL 2R . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
BALB / cJ mouse bone marrow derived mast cells ( BMMC ) developed with interleukin ( IL ) 3 can be stimulated by c kit ligand ( KL ) in the presence of IL 10 and IL 1beta for sequential immediate and delayed generation of prostaglandin ( PG ) D 2 through utilization of constitutive prostaglandin endoperoxide synthase ( PGHS ) 1 and induced PGHS 2 , respectively ( Murakami , M . , Matsumoto , R . , Austen , K . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
METHODS : The distribution of the c kit receptor was studied using specific antibodies in 5 normal patients , 10 patients with Hirschsprung ' s disease , and 3 patients with diversion loop enterostomies . c kit immunohistochemistry was also combined with reduced nicotinamide adenine dinucleotide phosphate diaphorase histochemistry or with c kit ligand ( stem cell factor ) immunohistochemistry . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In contrast , in mice treated with either GM CSF , GM CSF plus IL 4 , c kit ligand ( c kitL ) , or G CSF , class II+ CD11c+ cells were not significantly increased . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In this study , the effects of c kit ligand ( KL ) and flt 3 ligand ( FL ) were compared in the presence of IL 3 , GM CSF , and erythropoietin ( 3 / GM / EPO ) , using frequent medium exchange cultures of human bone marrow mononuclear cells ( BMMNC ) and CD 34 enriched cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Apheresis products contained 0 . 7 + / 0 . 2 % SD myeloma cells ( range 0 . 2 % 2 . 7 % ) , which demonstrated the capacity of plasma cells to proliferate , differentiate , and mature in response to c kit ligand ( SCF ) , IL 3 , IL 6 , and a combination of IL 3 and IL 6 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
When selected PB CD34+ cells that were expanded ex vivo with combinations of human hematopoietic growth factors including the c kit ligand ( KL ) , interleukin ( IL ) 1 beta , IL 3 , IL 6 , erythropoietin ( EPO ) and granulocyte macrophage colony stimulating factor ( GM CSF ) for 14 days were grafted to cotransplant carrying SCID mice , a considerable loss of their proliferative potential was observed regardless of the HGF combination used . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We have investigated the mobilization of these cells into the blood of patients with ovarian carcinoma randomized to receive granulocyte colony stimulating factor ( G CSF ; 5 micrograms / kg ) plus different doses of stem cell factor ( SCF ; c kit ligand ) after chemotherapy or G CSF alone after chemotherapy . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We have performed a comprehensive analysis of cell lines and tissues to compare and contrast the expression patterns of Flt 3 ligand ( FL ) , c Kit ligand ( KL ) , and macrophage colony stimulating factor as well as their receptors , Flt 3 , c Kit , and c Fms . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In quiescent HPCs the receptors for early acting [ flt 3 ligand ( FL ) , c kit ligand ( KL ) , bFGF , interleukin 6 ( IL 6 ) ] and multilineage [ IL 3 , granulocyte macrophage CSF ( GM CSF ) ] HGFs are expressed at significant levels but with different patterns , eg , kit and flt 3 are detected on a majority and minority of HPCs , respectively , whereas IL 3Rs and GM CSFRs are present on almost all HPCs . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Recent data suggest that local overexpression of the tissue hormone c kit ligand ( stem cell factor [ SCF ] ) is associated with accumulation of mast cells ( MCs ) and a decrease in expression of c kit in the accumulated MCs [ 28 ] . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The c kit ligand potentiates the allogeneic mixed lymphocyte reaction . ^^^ We have previously demonstrated that c kit ligand ( KL ) can enhance IL 2 induced proliferation in a subset of human natural killer cells expressing the c kit tyrosine kinase receptor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Immature human mast cells , arising from cord blood mononuclear cells cultured in the presence of the c kit ligand of human or murine origin , contain mixtures of morphologically immature and mature secretory granules . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
CD56bright natural killer cell subsets : characterization of distinct functional responses to interleukin 2 and the c kit ligand . ^^^ However , each CD56bright NK cell subset has distinct functional responses to IL 2 , the c kit ligand ( KL ) , or both . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We observed no significant alterations in bone marrow stromal cell proliferation or the production of IL 6 , GM CSF , soluble c kit ligand and TNF alpha following HCMV replication in either stimulated lipopolysaccharide ( LPS ) or unstimulated conditions . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Activity was preserved near input levels with combined murine c kit ligand ( KL ) , interleukin 1 ( IL 1 ) , IL 6 , and IL 11 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
C kit ligand ( Stem Cell Factor ) affects neuronal activity , stimulates pituitary adrenal axis and prolactin secretion in rats . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mouse bone marrow derived mast cells undergo exocytosis , prostanoid generation , and cytokine expression in response to G protein activating polybasic compounds after coculture with fibroblasts in the presence of c kit ligand . ^^^ We now demonstrate that mouse bone marrow derived mast cells ( BMMC ) developed in IL 3 , an immature mast cell population lacking responsiveness to the Gi coupled polycationic mast cell activators , underwent maturation toward a connective tissue type mast cells like phenotype that responded to polycationic compounds after only 4 to 6 days of coculture with Swiss 3T3 fibroblasts in concert with recombinant soluble c kit ligand ( KL ) , whereas 3T3 or KL alone was insufficient to mediate this process . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Addition of interleukin 3 ( IL 3 ) , stem cell factor ( c kit ligand ) and PIXY 321 appear to open up new vistas by enforcing trilineage and multilineage haematopoietic reconstitution . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Kit ligand ( KL , c kit ligand ) mRNA was detected in the ovaries of 26 day old prepubertal rats using in situ hybridization . ^^^ Kit ligand ( KL , c kit ligand ) mRNA was detected in the ovaries of 26 day old prepubertal rats using in situ hybridization . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Indeed , it has been shown that tyrosine kinase dependent stimulation of CD34+ hematopoietic cell lines with c kit ligand ( KL ) , IL 3 or GM CSF transiently activates the ability of VLA 4 to bind to VCAM 1 or FN . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In response to vascular endothelial growth factor and c kit ligand these precursors give rise to colonies containing immature cells ( blasts ) expressing marker genes characteristic of haematopoietic precursors . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
MGDF also enhanced interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , c kit ligand ( KL ) and FLT 3 ligand ( FL ) stimulated blast cell proliferation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The effects of Flk 2 / flt3 ligand as compared with c kit ligand on short term and long term proliferation of CD34+ hematopoietic progenitors elicited from human fetal liver , umbilical cord blood , bone marrow , and mobilized peripheral blood . ^^^ The Flk 2 / flt3 ligand ( FL ) was evaluated and compared with c kit ligand ( KL ) for its in vitro proliferative effects on CD34+ cells from human fetal liver , umbilical cord blood , bone marrow , and mobilized peripheral blood . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Glucocorticoids decrease tissue mast cell number by reducing the production of the c kit ligand , stem cell factor , by resident cells : in vitro and in vivo evidence in murine systems . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We and others have shown that the growth and differentiation of immature , nontransformed mouse bone marrow derived mast cells ( mBMMC ) can be regulated in vitro by IL 3 , IL 10 , and c kit ligand . ^^^ We now report that glucocorticoids inhibit the c kit ligand and IL 3 induced proliferation of mBMMC , the Fc epsilonRI mediated expression of TNF alpha , and the IL 10 mediated expression of the two chymases designated mouse mast cell protease ( mMCP ) 1 and mMCP 2 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Functional and ultrastructural studies on the burst of c kit ligand containing secretory cytoplasmatic vesicles . ^^^ These cells start to differentiate into hematopoietic progenitor precursors under the influence of c kit ligand , functioning in an autocrine and paracrine fashion . ^^^ The message for c kit ligand is transcribed in the fibroblast like cell line and the ligand for the tyrosine kinase c kit is expressed on the cell surface as well as secreted in its soluble form . ^^^ With a monoclonal antibody directed against all isoforms of c kit ligand , the expression of c kit ligand was investigated functionally and topographically by electron microscopy . ^^^ Ultrastructural analysis revealed c kit ligand in electron dense vesicles which were transported to the cell surface and burst to release the soluble , non membrane bound isoform of c kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Tpo alone stimulated limited clonal growth , but synergized with c kit ligand ( KL ) , flt 3 ligand ( FL ) , or IL 3 to potently enhance clonogenic growth . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Of interest , all of these cells expressed the c kit ligand , Steel factor ( SF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
This observation was made after noting that the c kit ligand stem cell factor ( SCF ) could not prevent IL 3 deprivation induced mast cell apoptosis and that CFTL 15 cells did not proliferate in response to SCF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The RT PCR investigation evidences the mRNA encoding for bFGF , IL 7 , GM CSF , and SCF ( c kit ligand ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The proliferative effects of SP correlate with the induction of positive hematopoietic growth factors such as IL 3 , IL 6 , GM CSF and c kit ligand and the inhibitory effects by NK A correlate with the induction of two negative hematopoietic regulators , MIP 1 alpha and TGF beta . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
A selected population of C58 / J bone marrow cells ( Thy 1 ( low ) , CD 3 , CD 8 , B 220 ) that is enriched for pre T cell activity was propagated in vitro in medium supplemented with IL 3 and mast cell growth factor ( MGF , also referred to as stem cell factor , Steele factor and c kit ligand ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
HLA DR triggered inhibition of hemopoiesis involves Fas / Fas ligand interactions and is prevented by c kit ligand . ^^^ Inhibition was prevented , however , if c kit ligand ( stem cell factor ( SCF ) ) was added to cultures concurrently with H81 . 9 . ^^^ These findings show that HLA DR mediated signals inhibit hemopoiesis in human marrow by a mechanism involving Fas / Fas L dependent signals that are blocked by c kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In the absence of growth factors , gene transfer into BM or MPB CD34+ DR cells was generally poor , but following a 72 hr prestimulation it peaked at 38 % of total CD34+ DR bone marrow ( BM ) cells in the presence of the c kit ligand ( KL ) and at 31 % in the presence of IL 3 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Selected CD34+ cells from mobilized apheresis products were cultured in serum free or serum containing media supplemented with granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) , and stem cell factor ( SCF ; c kit ligand ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Functionally distinct apoptotic schedules were associated with different phases of DC development when multipotent CD34+ progenitor cells were treated with GM CSF + TNF + / SCF ( c kit ligand ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The protooncogene c kit and c kit ligand in human disease . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
COX 2 induction and the attendant delayed PGD 2 generation in CTMC were modestly induced by c kit ligand , but not by Fc epsilonRI cross linking . ^^^ This indicated that the stimulus specificity differed from that observed in the immediate phase , in which NGF , c kit ligand , and Fc epsilonRI cross linking , either in combination with each other or with lysoPS as a cofactor , elicited comparable levels of PGD 2 generation within 10 min , reaching 10 to 20 ng / 10 ( 6 ) cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
RT PCR analysis demonstrated human but not murine stem cell factor ( SCF ; also termed mast cell growth factor , c kit ligand ) mRNA in xenografts . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Furthermore , culture of BMMC with a combination of c kit ligand , interleukin 1beta and interleukin 10 for > 24 h led to an increase in plasma type PAF AH expression , accompanied by a reduction in stimulus initiated PAF production . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Effect of recombinant canine stem cell factor , a c kit ligand , on hematopoietic recovery after DLA identical littermate marrow transplants in dogs . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The observation that BALB / c mouse bone marrow derived mast cells developed with interleukin ( IL ) 10 and c kit ligand contain mMCP 9 transcript , whereas those developed with IL 3 do not , indicates that the expression of this particular chymase is regulated by the cytokine microenvironment . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Conversely , expansion was enhanced in vivo by administration of stem cell factor ( SCF , c kit ligand ) and interleukin 11 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The c Kit ligand , stem cell factor , did not influence drug efflux activity of the nine c Kit positive AML specimens tested . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The c kit ligand stem cell factor and anti IgE promote expression of monocyte chemoattractant protein 1 in human lung mast cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We first evaluated the proliferative response of 3 leukemic cell lines and 32 primary samples from AML patients to IL 9 alone and combined with rh IL 3 , granulocyte macrophage colony stimulating factor ( GM CSF ) and stem cell factor ( SCF , c kit ligand ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Culturing CD34+ cells with stem cell factor ( c kit ligand ) plus granulocyte macrophage colony stimulating factor resulted in the appearance of a significant proportion of cells expressing phenotypic markers characteristic of dendritic cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Increased growth promoting but not mast cell degranulation potential of a covalent dimer of c Kit ligand . ^^^ The native form of soluble c kit ligand ( KL ) is a noncovalent dimer . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Nearly all ( > 95 % ) of the CD 34 ( + ) Thy 1 ( + ) Lin cells divided by day 6 when cultured in thrombopoietin ( TPO ) , c kit ligand ( KL ) , and flk2 / flt3 ligand ( FL ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
This population was then used to evaluate the effects of supplemental cytokines ( interleukin 2 [ IL 2 ] , IL 3 , IL 7 , c kit ligand ) , FLT 3 ligand ( FL ) , and stroma derived factors on lymphoid differentiation in vitro . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Although several mutations are known to severely reduce the number of PGCs reaching and populating the genital ridges , the molecular identity of only two of these genes is known : the c kit receptor protein tyrosine kinase and the c kit ligand ( the steel factor ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In analyzing P tyr proteins in primary lin normal blasts in response to various hematopoietic cytokines , we found a striking similarity in the tyrosine phosphorylation of four major and three minor proteins after stimulation with c kit ligand ( KL ) and the P tyr proteins that are constitutively phosphorylated in primary primitive lin chronic phase CML blasts . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Flt3 / Flk2 ligand ( FL ) and stem cell factor ( SCF ; c kit ligand ) interact with different but related surface receptor tyrosine kinases expressed on early hematopoietic cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Surprisingly , the c kit ligand SCF was found to augment expression of MITF mRNA in bone marrow MNC . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In the erythroleukemia cell line TF 1 , recombinant human erythropoietin ( rHEpo ) , but not c kit ligand , enhanced the number of cells expressing the erythropoietin receptor ( EpoR ) , as measured by flow cytometric analysis of binding of the biotin labeled Epo . ^^^ Moreover , 125I Epo binding and Scatchard analyses , indicated that TF 1 cells , maintained in standard conditions with IL 3 , and those stimulated with c kit ligand , bear a single class of EpoR . ^^^ On the other hand , cells cultured in the presence of rHEpo had a higher number of receptors than IL 3 or c kit ligand stimulated cells , and had two binding sites with different affinities for the ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We have also explored whether the addition of early acting growth factors known to enhance myelopoiesis in long term culture , such as fibroblast growth factor ( b FGF ) , insulin growth factor ( IGF 1 ) , c kit ligand or stem cell factor ( SCF ) , and flk 2flt 3 ligand ( FL ) , can lengthen survival of CD34+ progenitors in these cultures . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , also known as c kit ligand , is now recognized as the key molecule responsible for mast cell proliferation and is known to exert its action through specific surface receptors . ^^^ These results could be explained either by a mutation leading to constitutive activation of c kit or overproduction of c kit ligand leading to bladder mast cell proliferation in IC . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Regulation of cyclooxygenase 2 and endogenous cytokine expression by bacterial lipopolysaccharide that acts in synergy with c kit ligand and Fc epsilon receptor 1 crosslinking in cultured mast cells . ^^^ LPS , although showing minimal effects , significantly augmented the c kit ligand ( KL ) or IgE dependent expression of cyclooxygenase ( COX ) 2 and the attendant delayed PGD 2 generation , with IL 10 and IL 4 acting as potentiating and inhibitory cytokines , respectively . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Expression of IL 4 , IL 10 , stem cell factor ( SCF or c kit ligand ) and IFN gamma mRNA was also examined in these two tissues . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The c kit ligand SCF upregulates uPAR expression , and the release of tPA from MC . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Other members of this family include M CSF and the c kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Blasts were cultured for 2 weeks in a combination of cytokines c Kit ligand , interleukin 3 and granulocyte macrophage colony stimulating factor ( SIGm mix ) together with all trans retinoic acid or 1alpha , 25 dihydroxy vitamin D 3 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Addition of IL 3 to the combination of steel factor ( SF , c kit ligand ) and IL 11 abrogated the B lymphoid potential of the marrow cells of both types of knock out mice as well as wild type mice . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Cord blood ( CB ) or bone marrow ( BM ) CD34+ cells were stimulated and transduced in the presence of three cytokines ( interleukin 3 [ IL 3 ] , IL 6 , and stem cell factor [ SCF ; c Kit Ligand ] ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
CD34+ cells purified from leukapheresis product were seeded at 1 10 10 ( 4 ) cells / mL in complete medium supplemented with GM CSF , TNF alpha , IL 4 , c kit ligand , and flt 3 ligand ( FL ) . ^^^ This pattern of expression of surface antigen was unchanged whether the c kit ligand and / or FL was added . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The growth of MEB clones required IL 7 and c Kit ligand , corresponding to normal pro B cells which express SHP 1 . ^^^ MEB cells were sensitive to starvation by either IL 7 or c Kit ligand , resulting in apoptotic death . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Demonstration of mast cell chemotactic activity in nasal lavage fluid : characterization of one chemotaxin as c kit ligand , stem cell factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The c kit ligand ( KL ) , also produced by stromal cells , enhances the expansion of NK cells from CD 34 ( + ) HPCs in the presence of IL 15 , but alone has no ability to differentiate NK cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
W / Wv mice are deficient in tissue mast cells , and mast cells cultured from these mice do not proliferate in response to the c kit ligand , stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The c kit ligand , stem cell factor , can enhance innate immunity through effects on mast cells . ^^^ We previously reported that repetitive administration of the c kit ligand , stem cell factor ( SCF ) , can increase mast cell numbers in normal mice in vivo . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Progenitors were cultured in limiting dilutions with interleukin 7 ( IL 7 ) , flt 3 ligand ( FL ) , c kit ligand ( KL ) , IL 3 , IL 2 , and AFT 024 , a murine fetal liver line , which supports culture of transplantable murine stem cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The tPA protein was detectable in MC conditioned medium by Western blotting and immunoassay , and the MC agonist stem cell factor ( c Kit ligand ) was found to promote the release of tPA from MC . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In vivo administration of c kit ligand ( KL ) expands early hemopoietic progenitors and stem cells and sensitizes clonogenic progenitors to 5 FU mediated cell death . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
This features the use of a serum free medium supplemented with the cytokines flt 3 ligand , IL 3 , stem cell factor ( c kit ligand ) and IL 2 , together with IL 7 or oncostatin M ( OM ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
By adding TPO to all possible combinations of GM CSF , IL 3 , and c kit ligand ( CKL ) in a suspension culture system , we found a significant increase in both relative and absolute numbers of cells in cultures containing TPO of the megakaryocytic lineage and CD34+ cells after 14 days of culture . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Using c kit overexpressing cells , we observed that the potential of TGF beta 1 to up regulate the expression of Bcl 2 and p 27 could be counteracted by the c kit ligand , stem cell factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF , c kit ligand ) is a multifunctional cytokine which can promote melanocyte and mast cell development . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Murine mast cell proliferation and maturation are regulated by two distinct cytokines , interleukin 3 ( IL 3 ) and the c kit ligand , stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
MPB purified CD34+ Thy 1+ cells were labeled with CFSE dye and cultured for 112 hours in serum deprived medium in the presence of the cytokine combinations of thrombopoietin ( TPO ) , flt 3 ligand ( FL ) , and c kit ligand ( KL ) , or TPO , FL , and interleukin 6 ( IL 6 ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Comparing several different combinations of cytokines added to 90 hour cultures of MPB CD 34 cells , thrombopoietin ( TPO ) , flt 3 ligand ( FL ) , and c kit ligand ( KL ) gave the best result , with the lowest percentage of apoptotic cells and a mean 1 . 2 fold increase in the number of CD34+ Thy 1+ cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
RESULTS : We report here the ability of recombinant swine interleukin ( IL ) 3 and c kit ligand ( KL ) to specifically enhance swine hematopoietic chimerism in this system . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Despite this low level expression , flt 3 L exerted synergistic effects with a combination of c kit ligand , IL 3 , IL 6 , GM CSF and G CSF , mainly to induce proliferation of CD34+ / CD38 cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Selected progenitors were then cultured in the mixed colony assay with incremental concentrations of combinations containing erythropoietin ( Epo ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) and c kit ligand . ^^^ However , for all colony types , responses to effective concentrations of c kit ligand corresponded with those of controls . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
It serves as a master regulator in modulating extracellular signals , such as those triggered by alpha MSH and c Kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The c kit gene encodes an RTK for stem cell factor ( SCF ) , ( c kit ligand , KL ) , both of which play a critical role in the differentiation and growth of haemopoietic stem cells ( HSCs ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Whereas c kit ligand ( KL ) , interleukin 3 ( IL 3 ) , and IL 6 promoted proliferation and maintenance or expansion of murine long term reconstituting activity and human LTC IC , they dramatically depleted ELTC IC activity . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We expanded CD 34 ( + ) cells from CB in serum free medium with thrombopoietin , flk 2 ligand , and c kit ligand , with or without IL 3 and found that CAFCs cultured in the absence of IL 3 had a chemotactic response equivalent to noncultured cells , even after 5 weeks . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
BMMC were cultured with WEHI 3 conditioned medium . c Kit ligand and IL 10 were obtained by their expression in baculovirus . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Dendritic cells generated from CD34+ progenitor cells with flt 3 ligand , c kit ligand , GM CSF , IL 4 , and TNF alpha are functional antigen presenting cells resembling mature monocyte derived dendritic cells . ^^^ The combination of cytokines flt 3 ligand + c kit ligand + granulocyte macrophage colony stimulating factor ( GM CSF ) + interleukin 4 ( IL 4 ) + tumor necrosis factor alpha ( TNF alpha ) was used to maximize the output of mature DCs in the culture of CD34+ cells while minimizing the production of monocytes . ^^^ Differentiation was marked by a time dependent increase in allostimulatory function , which , at its peak , was more potent than in cultures of DCs generated from monocytes with GM CSF + IL 4 , but was comparable on a cell to cell basis to that of mature monocytes cultured in flt 3 ligand + c kit ligand + GM CSF + IL 4 + TNF alpha . ^^^ Altogether , these results suggest that DCs generated from CD34+ cells of patients with breast cancer with flt 3 ligand , c kit ligand , GM CSF , IL 4 , and TNF alpha are competent antigen presenting cells , particularly for CD8+ cytotoxic T lymphocytes , and resemble mature monocyte derived DCs in the assays described here . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To study self renewal and differentiation of spermatogonial stem cells , we have transplanted undifferentiated testicular germ cells of the GFP transgenic mice into seminiferous tubules of mutant mice with male sterility , such as those dysfunctioned at Steel ( Sl ) locus encoding the c kit ligand or Dominant white spotting ( W ) locus encoding the receptor c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Eighteen AML samples were cultured for 7 d in serum free medium with or without a supplement of recombinant cytokines comprising c kit ligand , IL 3 and GMCSF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
TGF beta , GM CSF , c kit ligand , and IL 7 are produced constitutively and IL 1beta , IL 6 , IL 8 , TNF alpha , IFN gamma , and MIP 1 alpha are upregulated upon stimulation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) is a C kit ligand and plays an important role in immune system homeostasis in the gastrointestinal tract . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
HbF was moderately enhanced by interleukin 3 ( IL 3 ) and granulocyte macrophage colony stimulating factor treatment ( up to 5 % 8 % gamma globin content ) , while sharply reactivated in a dose dependent fashion by c kit ligand ( KL ) and NaB ( 20 % 23 % ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Over the past decade , a number of hematopoietic growth factors with thrombopoietic activity have been identified , including stem cell factor ( c kit ligand ) , interleukin ( IL ) 1 , IL 3 , IL 6 , and IL 11 , as well as thrombopoietin ( TPO ) and its derivatives . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Bone marrow and umbilical cord blood CD34+ cells were cultured in serum free medium under survival conditions with thrombopoietin ( Tpo ) alone , or under proliferating conditions with Tpo , c Kit ligand ( KL ) , and Flt 3 ligand ( FL ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Through such studies , c kit ligand ( KL ) , flt 3 ligand ( FL ) , thrombopoietin , and IL 11 have emerged as likely positive regulators of HSC self renewal . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In addition , KGF secreted by theca cells and c kit ligand secreted by granulosa cells play paracrine roles in the regulation of preantral follicle growth and development . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In addition to effects on the differentiation of megakaryocytes , TPO has also been shown to stimulate the proliferation of megakaryocytic progenitor cells , colony forming units megakaryocytes ( CFU MK ) , to act in synergy with interleukin 3 or c kit ligand and erythropoietin ( Epo ) to stimulate the development of early and the generation of late erythroid progenitor cells , and to affect the rate of entry into the cell cycle and proliferative capacity of hematopoietic stem cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
MEKK 2 gene disruption causes loss of cytokine production in response to IgE and c Kit ligand stimulation of ES cell derived mast cells . ^^^ Transcription of specific cytokines in response to IgE or c Kit ligand was markedly reduced in MEKK 2 ( / ) ESMC relative to wild type ESMC . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
One of the most important factors to influence mast cell number , function , and phenotype is the c kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
CD34+ hematopoietic cells were cultured in serum free medium with megakaryocyte growth and development factor ( MGDF ) alone for supporting the survival of primitive progenitors or with MGDF , c kit ligand ( KL ) and flt 3 ligand ( FL ) for inducing proliferation of primitive progenitors . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The effects of TGF beta 1 on Bcl 2 and p 27 expression as well as cell growth were abrogated by c kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Half of each specimen was snap frozen for AchE and NADPH diaphorase histochemistry , and the other half were fixed with 10 % formalin for H & E staining and immunohistochemistry using PGP9 . 5 antibody ( a marker for neurons ) , C kit antibody ( a marker for intestinal pacemaker cells ) , and stem cell factor antibody ( a marker for C kit ligand ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
KIT and c KIT ligand ( SCF ) in synovial sarcoma ( SS ) : an mRNA expression analysis in 23 cases . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Among various combinations , the combination of c kit ligand , flt 3 ligand , c mpl ligand ( TPO ) , and interleukin ( IL ) 4 most potently generated the number of CD1a+CD14 DCs in cultures containing granulocyte macrophage colony stimulating factor ( GM CSF ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^ These findings demonstrated that the combination of c kit ligand plus flt 3 ligand plus TPO with GM CSF plus TNF alpha , followed by IL 4 , is useful for ex vivo generation of human DCs from mobilized CD34+ peripheral blood progenitors . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Here we show that stimulation of Mo 7 hematopoietic cells with c Kit ligand ( KL ) induces phosphatidylinositol ( PI ) 3 kinase dependent tyrosine phosphorylation and membrane recruitment of Dok 1 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Ex vivo expansion of stem and progenitor cells in co culture of mobilized peripheral blood CD34+ cells on human endothelium transfected with adenovectors expressing thrombopoietin , c kit ligand , and Flt 3 ligand . ^^^ Immortalized human bone marrow endothelial cells ( BMEC ) transfected with an expression vector carrying cDNA encoding the human telomerase reverse transcriptase ( hTERT ) and human umbilical vein endothelial cells ( HUVEC ) were transfected with combinations of adenovectors expressing murine c kit ligand ( KL ) , human thrombopoietin ( TPO ) , human Flt 3 ligand ( FL ) , and human granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Although mRNA for IL 3 and GM CSF was never detected , transcripts for c kit ligand and , more commonly , its receptor were . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
After 3 wk of culture in the presence of c kit ligand , IL 16 induced the progenitors residing in the blood of normal individuals to increase their expression of chymase and tryptase about 20 fold . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Cell surface immunophenotyping of spleen derived fibroblasts revealed a low and consistent expression of IL 15 , Flt 3 ligand , and c kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Sl / Sl ( d ) mutant mice offer a model system in which lack of transmembrane type c kit ligand ( KL 2 ) expression on the somatic Sertoli cell surface results in disruption of spermatogenesis . ^^^ Because adenoviral vectors showed toxicity , lentiviral vectors were used to express the c kit ligand in Sl / Sl ( d ) Sertoli cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Cryosections were immunofluorescently labelled using antibodies against connexins 43 , 40 and 45 , vimentin , desmin and c Kit ligand , and examined by confocal microscopy . ^^^ The connexin43 / vimentin positive cells showed only weak labelling for desmin and c Kit ligand , and were immunonegative for connexins 40 and 45 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Consistent with increased c Kit expression , KHSV infected DMVEC displayed enhanced proliferation in response to the c Kit ligand , stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
METHODS : Human mobilized peripheral blood ( MPB ) CD 34 ( + ) cells were cultured with the cytokines thrombopoietin mimetic peptide ( mTPO ) , flt 3 ligand ( FL ) , and c kit ligand ( KL ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
This study investigated early ex vivo expansion kinetics of UCB AC133+ cells ( 2 4 10 10 ( 4 ) / ml ) , mononuclear cells ( MNC , 1 2 10 10 ( 6 ) / ml ) and AC133negative cells ( AC 133 ( neg ) , 2 4 10 10 ( 4 ) / ml ) in stroma free 8 d liquid culture ( fetal bovine serum supplemented Iscove ' s modified Dulbecco ' s medium ( IMDM ) with either ' K36EG ' [ c Kit ligand , interleukin 3 ( IL 3 ) , IL 6 , erythropoietin , granulocyte colony stimulating factor ] or ' TPOFL ' ( thrombopoietin , Flt 3 ligand ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Levels of E2A protein expression in B cell precursors are stage dependent and inhibited by stem cell factor ( c kit ligand ) . ^^^ The expression of E2A protein and mRNA during early B lymphopoiesis was determined and effects of stem cell factor ( SCF ; Steel factor ; c kit ligand ) on E2A expression were evaluated . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
By Western blotting and immunohistochemistry , expression levels of the c kit ligand , stem cell factor , in skin and epidermis are strongly increased after cyclophosphamide treatment . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
INTRODUCTION : We compared the effects of the early acting growth factors ( GF ) , Flt 3 ligand ( FL ) , c Kit ligand ( KL ) , and leukemia inhibitory factor ( LIF ) , and the late acting GF , granulocyte colony stimulating factor ( G CSF ) and megakaryocyte growth and development factor ( MGDF ) , added alone in human long term marrow culture ( LTMC ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The adherent cells were differentiated into DCs within 2 weeks by adding granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 4 ( IL 4 ) , c kit ligand and tumour necrosis factor alpha ( TNF alpha ) . ^^^ CONCLUSIONS : Our findings indicate that plastic adherent peripheral blood cells , when cultured with GM CSF , IL 4 , c kit ligand and TNF alpha in autologous human plasma supplemented media , are a potent source of functional DCS that may be of value for human therapy . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The present report describes the enumeration , phenotyping and isolation of porcine haematopoietic progenitor cells expressing stem cell factor ( SCF , c kit ligand ) receptor ( c kit , CD 117 ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
At 4 h , the combination of the two growth factors induced a number of chemokine and cytokines and their receptors ( IL 8 , IL 6 , IL 11 , CCR 6 , CXCR 1 , CXC 1 and IL17RC ) , numerous genes involved in growth factor signal transduction ( egr 1 , fosB , grb 10 , grb 14 , MAP2K3 , MAP3K8 , MAPKAP 2 , MPK 3 , DUSP 4 and DUSP 6 ) , as well as a number of other growth factors ( PDGFA , BMP 2 , Hb EGF , FGF 16 , heuregulin beta 1 , c kit ligand , angiopoietin 2 and angiopoietin 4 and VEGFC ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Moreover , we found that estrogens stimulate the transcription of the Steel gene and the production of c Kit ligand in gonadal somatic cells , and that this growth factor is likely to be responsible for the observed stimulation of PGC growth via an Akt / PTEN pathway . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Compared with TACE ( + / + ) cells , TACE ( DeltaZn / DeltaZn ) mast cells also demonstrated decreased constitutive shedding and increased basal surface expression of c Kit , with diminished apoptosis in response to c Kit ligand deprivation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
METHODS : AC 133 ( + ) stem / progenitor cells from six full term pregnancies UCB samples were immunomagnetically selected , followed by ex vivo expansion culture in the presence of thrombopoietin ( TPO ) , c kit ligand ( KL ) , flt 3 ligand ( FL ) and IL 6 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Furthermore , over 8 weeks in liquid culture stimulated by a cytokine cocktail optimized for HSPC expansion , TPOFLK ( thrombopoietin 10 ng / ml , Flt 3 ligand 50 ng / ml , c Kit ligand 20 ng / ml ) Lin cells underwent slow proliferation but maintained / expanded more primitive HSPC than CD133+ cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In vitro generation of DC was achieved using a cytokine cocktail containing tumor necrosis factor alpha , granulocyte macrophage colony stimulating factor , c kit ligand , and soluble CD 40 ligand ; yet , addition of the HDAC inhibitor ( Hdi ) trichostatin A enhanced DC differentiation with retention of the fusion transcripts . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Evidence provided that the most efficient long term outgrowth of DC progenitors was obtained by GM CSF independent culture systems with the aid of Flt3 / Flk 2 and IL 6 , not with c kit ligand and IL 6 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Retroviral transduction of CD 34 ( + ) hemopoietic progenitor cells , during pre expansion by thrombopoietin , c kit ligand , and FLT 3 ligand , on recombinant fibronectin , but in the absence of FCS , resulted in gene expression by 20 30 % of the LCs . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We investigated the effect of SCF , a c kit ligand , on the radiosensitivity of HL 60 cells . 10 ray induced apoptosis in HL 60 cells was significantly lower in the presence of SCF than in the absence of SCF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Genes that showed decreased expression included the chemokine receptor CXCR 4 , ciliary neurotrophic factor ( CNTF ) , c kit ligand , Ephrin A 5 , G protein coupled receptor RDC 1 and FGF family FGFR 2 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Embryos were produced by SCNT , intracytoplasmic sperm injection ( ICSI ) or under different conditions , and transcripts of genes for fibroblast growth factor receptor ( FGFr ) 2IIIc , FGFr72IIIb , 10 inactive specific transcript ( Xist ) , interleukin 6 ( IL 6 ) , IL 6 receptor ( IL6r ) alpha and c kit ligand , were detected by real time RT PCR . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
After 7 days of high density culture in microflasks , ( 10 ( 5 ) cells / ml , IMDM , FCS 10 % , thrombopoietin 10 ng / ml , flt 3 ligand 50 ng / ml , c kit ligand 20 ng / ml ) . ^^^ The CBEs were also microgravity bioreactor cultured with hepatocyte growth medium ( IMDM , FCS 10 % , HGF 20 ng / ml , bFGF 10 ng / ml , EGF 10 ng / ml , c kit ligand 10 ng / ml ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
METHODS : Purified murine HSC with the phenotype c Kit+ , Thy 1 . 1 ( lo ) , Lin ( / lo ) , Sca 1+ were cultured for 4 days with IL 3 , IL 6 and c Kit ligand without or with HDAC inhibitors , after which their degree of phenotypic differentiation in culture was assessed by flow cytometric analysis . ^^^ To explore whether HDAC inhibitors could have a beneficial role in human HSC transplantation , mobilized peripheral blood CD34+ cells were cultured with thrombopoietin mimetic peptide , flt 3 ligand , and c Kit ligand , without or with various HDAC inhibitors . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Although the c Kit ligand stem cell factor ( SCF ) induces the phosphorylation of protein kinase B ( or Akt ) and prevents apoptosis in Schwann cells , SCF has no effect on the proliferation or differentiation of Schwann cells . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The importance of early hematopoietic growth factors , such as c kit ligand and flt 3 ligand , and their synergy with IL 15 in the development of human NK cells in the bone marrow has permitted the investigation of novel cytokine combinations for optimizing in vivo expansion of NK cell in the clinic . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Soluble c kit ligand production by bone marrow stromal cells is independent of the degree of neutropenia in patients with chronic idiopathic neutropenia . ^^^ The levels of soluble c kit ligand ( sKL ) , also known as Steel factor or stem cell factor , were measured in blood serum and long term bone marrow culture supernatants of 81 patients with chronic idiopathic neutropenia ( CIN ) and 22 normal controls using a commercially available enzyme linked immunosorbent assay ( ELISA ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
BACKGROUND : Vascular endothelial growth factor ( VEGF ) , basic fibroblast growth factor ( bFGF ) , transforming growth factor beta 1 ( TGF beta 1 ) , endothelin 1 ( ET 1 ) and soluble c kit ligand ( sKL ) are cytokines involved in embryogenesis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
A modified whole blood fixation protocol was developed for the AML / MDS trial , using the c kit ligand stem cell factor ( SCF ) to activate ERK as an alternative to PMA , and incorporating immunophenotypic markers to identify leukemic blasts . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To better understand how RabGEF 1 can regulate signaling events and biological responses in mast cells , we examined the responses of bone marrow derived cultured mast cells ( BMCMCs ) from wild type ( + / + ) and Rabgef 1 knockout ( / ) mice after stimulation with the c Kit ligand , stem cell factor ( SCF ) , an important regulator of mast cell development , survival , proliferation , and activation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The receptor tyrosine c Kit and its cognate ligand , c Kit ligand ( KL , stem cell factor , SCF ) , are involved in ovarian follicular development in several animal species . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Osteopetrotic mouse stroma with thrombopoietin , c kit ligand , and flk 2 ligand supports long term mobilized CD34+ hematopoiesis in vitro . ^^^ To evaluate the OP 9 capability to sustain long term hematopoiesis , we reported the expansion of granulocyte colony stimulating factor ( G CSF ) mobilized human peripheral blood ( PB ) CD 34 ( + ) cells in co culture with murine OP 9 and MS 5 stromal cells , either transfected with various combinations of adenovectors ( Ad ) expressing c kit ligand ( KL ) ( either soluble or transmembrane form ) , thrombopoietin ( TPO ) , flt 3 / flk2 ligand ( FL ) , and granulocyte macrophage ( GM ) CSF or with weekly addition of these cytokines . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We therefore studied whether culturing spleen or bone marrow ( BM ) HSC in medium containing 5 azacytidine could induce a cardiac phenotype . c kit enrichment and depletion of adult pig spleen and BM mononuclear cells were obtained by magnetic bead separation using biotinylated pig stem cell factor ( c kit ligand ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Neither granulocyte macrophage colony stimulating factor , interleukin 3 , interleukin 6 , nor the kit ligand induced the phosphorylation of the M ( r ) 75 , 000 receptor protein , although these growth factors induced the phosphorylation of other proteins . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Numerous studies attest to the importance of the c kit receptor and its ligand , known variously as stem cell factor ( SCF ) , mast cell growth factor ( MGF ) , Steel factor ( SF ) , or kit ligand ( KL ) ( the nomenclature we prefer ) , in the development of human hematopoietic cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Expression of two types of kit ligand mRNAs in human tumor cells . ^^^ The primary structure of human kit ligand ( KL ) growth factor mRNA was analyzed by polymerase chain reaction ( PCR ) and nucleotide sequencing . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Recent reports by several groups that the SI locus encodes a protein known variably as stem cell factor ( SCF ) , mast cell growth factor , or kit ligand , suggests the possibility that the lack of wild type SCF in SI / SI ( d ) mice is responsible for their defective response to 5FU induced thrombocytopenia . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Reverse transcriptase PCR further revealed that no transcripts for c kit ( the receptor for kit ligand ) and the IL 7R could be detected in these cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Interleukins 9 , 10 , 11 and 12 and kit ligand : a brief overview . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The W locus in murine chromosome 5 encodes the c kit proto oncogene and the Sl locus in chromosome 10 encodes the ligand for c kit , which has been named stem cell factor ( SCF ) , mast cell growth factor ( MGF ) , kit ligand ( KL ) and steel factor ( SL ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Several cDNAs for the Sl gene product , known as mast cell growth factor ( MGF ) , stem cell factor ( SCF ) , or kit ligand ( KL ) , have recently been isolated , and both soluble and membrane associated versions have been shown to be biologically active . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
This factor has been referred to as mast cell growth factor , stem cell factor , or kit ligand , and will be referred to here as steel factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We show that normal human melanocytes respond to mast cell growth factor ( MGF ) , the Kit ligand that stimulates phosphorylation of tyrosyl residues in c Kit and induces sequential phosphorylation of tyrosyl residues in several other proteins . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Two such cultures spontaneously gave rise to factor independent variants which grew slowly in unsupplemented liquid culture and formed agar colonies , yet still responded positively to IL 7 and kit ligand , and negatively to gamma interferon . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
BALB / 3T3 CM contained the mast cell growth factor ( MGF ) / stem cell factor ( SCF ) / kit ligand ( KL ) , which is the ligand for the receptor encoded by the W ( c kit ) gene . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Kit ligand is a novel polypeptide growth factor which binds and activates the c kit protooncogene , a receptor tyrosine kinase . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
AIC2A and AIC2B transcription was transiently induced in these cells in response to a variety of hematopoietic growth factors , including erythropoietin ( EPO ) , monocyte CSF , IL 3 , GM CSF , and stem cell factor ( SCF or kit ligand ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Interleukin ( IL ) 7 has been shown to stimulate proliferation of pre B cells following c mu expression and this proliferative stimulus is potentiated by kit ligand ( KL ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
This approach should be generally applicable for defining activating mutations in related growth factor receptors , including those for platelet derived growth factor and Steel factor ( KIT ligand ) , in which ligand independent oncoprotein variants have not been identified . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The kit ligand : a cell surface molecule altered in steel mutant fibroblasts . ^^^ We have characterized the kit ligand by a generally applicable approach : the receptor extracellular domain was genetically fused to placental alkaline phosphatase , producing a soluble receptor affinity reagent with an enzyme tag that could be easily and sensitively traced . ^^^ These findings provide direct molecular evidence that the kit ligand can exist as a cell surface protein . ^^^ Binding was not detected on 3T3 fibroblasts carrying the steel ( Sl ) mutation , confirming the biological significance of the binding activity and demonstrating that mutations at the Sl locus affect the expression or structure of the kit ligand . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Transmembrane form of the kit ligand growth factor is determined by alternative splicing and is missing in the Sld mutant . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
B8 was able to inhibit the binding of the kit ligand to HEL cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Steel factor ( SLF ) ( aka : mast cell growth factor , stem cell factor , kit ligand ) is the product of the murine Steel locus on chromosome 10 and is a ligand for the c kit protooncogene . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The recent molecular analysis of several White Spotting and Steel alleles has provided insights into the mechanism of c kit ligand mediated processes , including cell proliferation , cell migration and cell survival . ^^^ Furthermore , the availability of the kit ligand has allowed in vitro investigations of the pleiotropic functions of c kit in development and cell differentiation to be carried out . . ^^^ The kit ligand encoded at the murine Steel locus : a pleiotropic growth and differentiation factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Biologically active kit ligand growth factor is produced by mouse Sertoli cells and is defective in SId mutant mice . ^^^ In order to define the role of Kit ligand ( KL ) growth factor encoded at the mouse steel ( SI ) locus in spermatogenesis , we have examined its production in Sertoli cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The growth factor receptor phenotype of this immature cell fraction has been determined for granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) , and IL 6 as well as for kit ligand , making c kit an especially strong growth factor receptor marker for reconstituting stem cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Isolation of endothelial like stromal cells that express Kit ligand and support in vitro hematopoiesis . ^^^ In accord with this finding , Northern blots of mRNA from unstimulated EL cells demonstrate constitutive expression of Kit ligand ( KL ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Because alterations in the relative levels of expression of Kit and KitA may affect the ability of a cell to respond to the Kit ligand , Steel factor , we examined the Kit / KitA RNA ratio in AML patients that differed with respect to a number of diagnostic , prognostic , and biologic parameters . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Interferon gamma and kit ligand are primary regulators of GTP cyclohydrolase activity : mechanisms and implications . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The kit ligand , stem cell factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The results indicate that , upon stimulation of cells with kit ligand , 1 ) the wild type c kit protein was readily autophosphorylated and autophosphorylation was not diminished significantly with any of the mutant proteins ; 2 ) p 85 and PI 3 kinase activity associated with wild type c Kit protein as well as with the mutant proteins YF 702 , YF 728 , and YF 745 . ^^^ One of the immediate events after binding of the kit ligand to the receptor is its association with the 85 kDa subunit ( p 85 ) of the phosphatidylinositol ( PI ) 3 kinase and the activation of the enzyme . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Steel factor ( SF ) , also referred to as Kit ligand , stem cell factor , or mast cell growth factor , is essential for the development of hematopoietic stem cells in vivo . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Role of kit ligand in proliferation and suppression of apoptosis in mast cells : basis for radiosensitivity of white spotting and steel mutant mice . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
These include kit ligand ( KL ) , interleukins ( IL ) 1 , 3 and 6 and granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Using biotin labeled human Kit ligand ( KL ) and flow cytometry , Kit was detectable on 2 % to 3 % of the nucleated cells in rhesus monkey bone marrow . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The kit ligand ( steel factor ) and its receptor c kit / W : pleiotropic roles in gametogenesis and melanogenesis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
CD 34 ( 2+ ) CD 38 cells needed at least the presence of interleukin 3 ( IL 3 ) and kit ligand ( KL ) together with either IL 1 , IL 6 , or granulocyte colony stimulating factor ( G CSF ) in the primary liquid phase in order to proliferate and differentiate into secondary colony forming cells ( CFC ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In addition , rapamycin blocks the proliferative response of cell lines to a variety of hematopoietic growth factors , including interleukin 3 ( IL 3 ) , interleukin 6 ( IL 6 ) , granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and kit ligand ( KL ) , suggesting that it should be a strong inhibitor of hematopoiesis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Expression of c kit and kit ligand proteins in normal human tissues . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To study the migration of human mast cells we used as a model the human mast cell line , HMC 1 , and stem cell factor dependent ( also referred to as mast cell growth factor or Kit ligand ) cord blood derived mast cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In the wild type mouse ( C57BL / 6 ) , interferon gamma and kit ligand induce GTP cyclohydrolase 1 activity in primed T cells and in bone marrow derived mast cells , respectively . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The receptor tyrosine kinase Kit and Kit ligand ( KL ) , encoded at the murine white spotting ( W ) and steel ( Sl ) loci , respectively , function in hematopoiesis , melanogenesis , and gametogenesis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Expression of c kit and kit ligand at the human maternofetal interface . ^^^ Kit ligand , or stem cell factor , is a recently identified growth factor , which binds to and activates the c kit proto oncogene , and which has been shown to act synergistically with other haematopoietic growth factors in the bone marrow . ^^^ In order to elucidate the role of c kit and its ligand during human placental development we have investigated the expression of c kit and kit ligand in human first trimester and term placenta as well as in pregnant and non pregnant endometrium , by immunocytochemistry and flow cytometric analysis . ^^^ Kit ligand was also expressed throughout pregnancy by invasive fetal extravillous trophoblast , and by fetal fibroblasts within the placental villi . c kit was found to be expressed on Hofbauer cells within the chorionic villi , and by decidual macrophages at all stages in pregnancy . c kit was also detected on the small CD56dim subset of uterine large granular lymphocytes which form the major leukocyte population in human first trimester decidua . ^^^ Our results suggest that kit ligand may be involved in the regulation of fetal macrophages , and in particular in signalling between invading extravillous trophoblast which expresses kit ligand , and maternal leukocytes bearing the c kit receptor . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
KIT constitutes the cell surface transmembrane receptor protein tyrosine kinase for a growth factor variously termed steel factor ( SLF ) , stem cell factor , mast cell growth factor , or Kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We found that culturing these cells for 1 to 3 days in Iscove ' s medium supplemented with kit ligand ( KL ) , interleukin 3 ( IL 3 ) , interleukin 1 beta ( IL 1 beta ) and 20 % bovine calf serum before freezing doubled the proliferative capacity of both myeloid and erythroid progenitor cells after thawing . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The precursor of the Kit ligand , SLF , is also a transmembrane protein that exists as a soluble factor as well as a cell surface protein . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Synergism between FL and either granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) or KIT ligand ( KL ) was observed in promoting the growth of high proliferative potential ( HPP ) colony forming cells ( CF ) and / or low proliferative potential ( LPP ) CFC in cultures of CD34++ CD38+ Lin and CD34++ CD 38 Lin LDFL cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) ( also known as kit ligand , mast cell growth factor and Steel factor ) is an early acting hematopoietic growth factor that is produced by a variety of mesenchymal cells including bone marrow stromal cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The pleiotropic effects of the Kit receptor system are mediated by Kit Ligand ( KL ) induced receptor autophosphorylation and its association with and activation of distinct second messengers , including phosphatidylinositol 3 ' kinase ( PI 3 kinase ) , p21ras and mitogen activated protein kinase ( MAPK ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
They were then stimulated with kit ligand ( KL ) , interleukin 3 ( IL 3 ) , and interleukin 1 beta ( IL 1 beta ) for 36 h either prior to cryopreservation or immediately after thawing . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
This production is enhanced in combination with Kit ligand or interleukin 3 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The results indicate that c kit positive type A spermatogonia in the testes of 5 day old mice require steel factor ( kit ligand ) for their proliferation , whereas self renewal and differentiation of c kit negative primitive type A spermatogonia in the testes of 2 day old mice do not . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Possible role of kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Human bone marrow cells that were responsive to the early acting cytokines Kit ligand and interleukin 3 were forced to a metabolic death . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
BMMC were grown for 2 wk in IL 3 alone and then cultured for 2 to 3 wk with kit ligand ( KL ) and / or IL 3 in the presence or absence of IL 4 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Transmembrane kit ligand cleavage does not require a signal in the cytoplasmic domain and occurs at a site dependent on spacing from the membrane . ^^^ The kit ligand ( KL ) is one of several growth factors that are active as transmembrane molecules and can also be proteolytically cleaved to yield soluble forms . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
A large fraction ( > 30 % ) of single cell / well sorted CD34bright / RhLA DRdull cells formed multilineage colonies after 2 to 4 weeks of stimulation with IL 3 , GM CSF , Kit ligand , and IL 6 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Further flow cytometric analysis using biotinylated growth factors showed that the IL 3R+ basophils also expressed receptors for granulocyte macrophage colony stimulating factor ( GM CSF ) , but not for IL 6 or Kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In these experiments HPCs / HSCs were treated with FL + / kit ligand ( KL ) + / monocyte colony stimulatory factor ( M CSF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The proliferation kinetics and clonogenic activity of CD34+ / 38hi ( CD38hi ) and CD34+ / 38lo ( CD38lo ) human marrow cells were measured before and after culturing the cells in vitro over a 6 day period in serum deprived medium containing recombinant growth factors ( interleukin 1 [ IL 1 ] , IL 3 , IL 6 , granulocyte colony stimulating factor [ G CSF ] , granulocyte macrophage [ GM ] CSF , kit ligand , and erythropoietin ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Among this group are Steel factor ( also known as mast cell growth factor , stem cell factor and kit ligand ) , and the more recently described flt 3 ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The objective of this study was to examine the expression and activation of the c kit receptor , a specific receptor for kit ligand ( stem cell factor , steel factor ) , in rat type A spermatogonia . ^^^ In an attempt to determine whether this receptor has a functional significance , we examined the effect of kit ligand on the phosphorylation of the c kit receptor . ^^^ The c kit receptor appeared to be constitutively autophosphorylated on tyrosine at low basal levels , and upon stimulation with kit ligand , the amount of phosphorylated protein increased significantly . ^^^ These observations indicate that kit ligand induces autophosphorylation of the c kit receptor , which may lead to the activation of other cellular target proteins responsible for spermatogonial proliferation and / or differentiation . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The granulomonocytic colony forming unit ( CFU GM ) assay measures committed progenitors responsive to a single species of colony stimulating activity such as granulocyte macrophage colony stimulating factor ( GM CSF ) , whereas the pre CFU assay identifies more primitive progenitors by measuring interleukin 3 ( IL 3 ) and kit ligand ( KL ) induced generation of secondary CFU GM from CD34+ , 4 hydroperoxycyclophosphamide resistant progenitors that require multiple cytokine stimuli . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Herein , we show that the cytokines interleukin ( IL ) 3 , granulocyte macrophage CSF ( GM CSF ) , and KIT ligand ( KL ) are physiological activators of VLA 4 and VLA 5 expressed by MO7e , TF 1 , and normal bone marrow CD34+ progenitor cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Most populations of human leukemic acute myeloblastic leukemia ( AML ) cells express c Kit on their surface and respond to Kit ligand ( KL ) in culture . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We also demonstrated that , in the presence of interleukin 3 , interleukin 6 , granulocyte colony stimulating factor , and erythropoietin , TGF beta 1 antisense oligonucleotides or anti TGF beta serum have an additive effect with KIT ligand ( Steel factor [ SF ] ) , which suggests that they control different pathways of regulation in these conditions . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Here we show that the KIT ligand SCF ( for stem cell factor ) can replace EPO in supporting the growth and survival of HCD 57 cells , an EPO dependent erythroid progenitor cell line expressing high levels of KIT . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
After more than a decade since Whitlock and Witte established an in vitro long term culture of bone marrow B lineage cells , c kit+IL 7 receptor ( R ) +B precursor cells in fresh bone marrow are now able to be grown under a fully defined culture condition containing only BSA , transferrin , IL 7 and the ligand for c kit ( Kit ligand ; KL ) as protein components . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Finally , we observed that kit ligand ( KL ) , known to synergize with IL 7 in the most primitive stages of normal B cell development , did not enhance the IL 7 responses of BCP ALL cells . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To determine if FGF was capable of stimulating multipotent progenitors , we assessed the output of different colony types after stimulation of BM mononuclear cells ( BMMC ) or CD34+ BMMC and compared the effects of FGF with the stem cell active cytokine , kit ligand ( KL ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Kit ligand in synergy with interleukin 3 amplifies the erythropoietin independent , globin synthesizing progeny of normal human burst forming units erythroid in suspension cultures : physiologic implications . ^^^ In suspension cultures containing no erythropoietin ( Epo ) , we have found that kit ligand ( KL ) in synergy with interleukin 3 not only increases the proliferation of cells and of progenitors but also consistently amplifies a population of cells that contain globin within 1 week . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The murine steel panda mutation affects kit ligand expression and growth of early ovarian follicles . ^^^ To investigate the basis of the phenotype of the Slpan mutation , the coding region of the kit ligand ( KL ) in Slpan / Slpan animals was characterized and shown to be identical to that from + / + mice . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In a concerted action kit ligand and interleukin 3 control the synthesis of serotonin in murine bone marrow derived mast cells . ^^^ Up regulation of GTP cyclohydrolase 1 and tryptophan 5 monooxygenase activity by the kit ligand . ^^^ In this study we demonstrate that among a panel of growth promoting cytokines including kit ligand ( KL ) , interleukin 3 ( IL 3 ) , IL 4 , IL 9 , and nerve growth factor , KL selectively enhances the synthesis of H4biopterin through up regulation of GTP cyclohydrolase 1 activity to 6 . 2 fold levels . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mechanism of kit ligand , phorbol ester , and calcium induced down regulation of c kit receptors in mast cells . ^^^ In an effort to study the function of the c kit receptor , specifically the physiological mechanism of controlling the signal induced by the ligand , the effect and mechanism of down regulation of the c kit receptor by the kit ligand ( KL ) was investigated in mast cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The W mutation involves the gene for c kit , and the S 1 mutation the gene for the kit ligand , called mast cell growth factor , steel factor , or stem cell factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Consistent with the cell autonomous and microenvironmental nature of W and Sl mutations , respectively , W encodes the c kit receptor tyrosine kinase while Steel encodes the Kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF , also known as mast cell growth factor , Steel factor or Kit ligand ) has been found to synergize with GM CSF , IL 6 , IL 3 , IL 11 or Epo to increase the numbers of megakaryocyte containing colonies ( i . e . , CFU Meg , BFU Meg , CFU GMM , CFU GEMM ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Since the expression of c kit in the stem cell fraction does not correspond with the poor response to the kit ligand stem cell factor ( SCF ) by these cells , we further analyzed the fractions with respect to their binding of biotinylated SCF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Several investigational rhHGFs , including interleukin 3 , 6 , and 11 , PIXY 321 ( a granulocyte macrophage colony stimulating factor / interleukin 3 hybrid ) , and kit ligand ( also known as `` stem cell factor ' ' ) , have the potential for affecting multiple lineages . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Expression of c kit and kit ligand in human colon carcinoma cells . ^^^ To determine whether c kit and kit ligand ( KL ) mRNAs could be expressed in human epithelial tumors , reverse transcriptase polymerase chain reaction and Northern blot analysis were performed . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Bone marrow cells from each of two inbred mouse strains , C57BL / 6J and DBA / 2J , were placed into separate bioreactor cultures perfused continuously with growth medium containing erythropoietin ( Epo ) , interleukin 3 ( IL 3 ) , granulocyte macrphage colony stimulating factor ( GM CSF ) , and Kit ligand as well as 5 % CO 2 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Simultaneously it was found that early erythroid progenitors did not respond , to the kit ligand , in the presence of TNF alpha . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In contrast , recombinant human Kit ligand induced the differentiation of mast cells that were tryptase positive but negative for chymase , carboxypeptidase , and Fc epsilon RI . ^^^ This data indicates that factors other than Kit ligand are critical for inducing the differentiation and maturation of mast cells in the human . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Two cell lines , HS 5 and HS 21 , appear to be fibroblastoid and secrete significant levels of granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage CSF ( GM CSF ) , macrophage CSF ( M CSF ) , Kit ligand ( KL ) , macrophage inhibitory protein 1 alpha , interleukin 6 ( IL 6 ) , IL 8 , and IL 11 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stimulation with an agonist antibody gives rise to an expansion of both myeloid and lymphoid cells and this effect is enhanced by the addition of kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Exogenously added stroma related factors such as kit ligand or IL 11 may be effective in correcting the deficiency . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Its expression is regulated by interferon gamma or kit ligand in a tissue specific manner . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
According to our results the best strategy to stimulate human megakaryocytopoiesis in vivo seems to be simultaneous or sequential use of the mixture of several cytokines including : interleukin 3 , interleukin 6 , erythropoietin or kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The FLT3 / FLK2 receptor tyrosine kinase is closely related to two receptors , c Kit and c Fms , which function with their respective ligands , Kit ligand and macrophage colony stimulating factor to control differentiation of haematopoietic and non haematopoietic cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The optimal combination was found to be : Interleukin 3 ( IL 3 ) + Granulocyte Monocyte Colony Stimulating Factor ( GM CSF ) + kit ligand ( KL ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
However , combinations of cytokines present in conditioned media from the T cell lines MO or C5MJ , or defined multiple cytokine combinations containing IL 1 , IL 3 , GM CSF , kit ligand , IL 6 , and IL 9 , restored myelopoiesis in vitro . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Bacterial endotoxin ( LPS ) modulation of CSF 1 , granulocyte macrophage ( GM ) CSF , G CSF , IL 1 , TNF , and Kit Ligand receptors on murine bone marrow cells ( BMC ) in vivo and in vitro was investigated . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Although cells grown from 5 of 6 AML patients were inhibited by TGF beta 1 , cells from 1 AML patient were growth stimulated by TGF beta 1 in the presence of granulocyte macrophage colony stimulating factor ( GM CSF ) , M CSF , or mast cell growth factor ( kit ligand ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In contrast , cultures lacking Epo ( ie , with interleukin 3 and kit ligand ) display limited progression towards both the myeloid and erythroid pathways , and high levels of expression of both GATA 1 and GATA 2 are maintained . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The initial stages of hematopoietic development within the EBs occur in the absence of added growth factors and are not significantly influenced by the addition of a broad spectrum of factors , including interleukin 3 ( IL 3 ) , IL 1 , IL 6 , IL 11 , erythropoietin , and Kit ligand . ^^^ At days 10 and 14 of differentiation , EB hematopoiesis is significantly enhanced by the addition of both Kit ligand and IL 11 to the cultures . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Cross reactivity between GFRs and heterologous GFs ( including IL 6 , IL 3 , GM CSF , Ep , and the kit ligand [ KL ] ) was explored by competition experiments on purified progenitors with radiolabeled and excess cold HGFs at +4 degrees C . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
By RT PCR analysis , constitutive mRNA expression of c kit , G CSF , GM CSF , IL 1 beta , IL 6 , IL 7 , IL 8 , IL 11 , Kit ligand ( KL ) , LIF , M CSF , MIP 1 alpha , TGF beta , and TNF alpha was demonstrated in both cell lines , with L87 / 4 a more potent cytokine producer than L88 / 5 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Receptor transmodulation between GM CSF , IL 3 , and stem cell factor ( or kit ligand ) was not detected on CD34+ cells in 48 hour cultures . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor , the kit ligand , induces direct degranulation of rat peritoneal mast cells in vitro and in vivo : dependence of the in vitro effect on period of culture and comparisons of stem cell factor with other mast cell activating agents . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The tyrosine kinase receptor c kit is expressed in type A , intermediate , and B spermatogonia , and kit ligand ( KL ) is expressed in Sertoli cells . ^^^ Kit ligand mediates survival of type A spermatogonia and dividing spermatocytes in postnatal mouse testes . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
While the levels of expression of several genes involved in erythropoiesis ( GATA 1 , NF E 2 , SCL , and EpoR ) were reduced in the livers of homozygous mutant fetuses in comparison to phenotypically normal littermates and one gene , Kit ligand ( KL ) , was expressed at higher levels in the mutant livers , these results must be viewed with caution . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Surprisingly , TNF alpha was a very potent stimulator of the proliferation of CD34++CD38 cells and was the most potent synergistic factor for the IL 3 induced proliferation of these cells of all cytokines tested ( IL 1 , IL 6 , granulocyte colony stimulating factor , kit ligand ) . ^^^ TNF alpha was the only cytokine that , as a single added factor , induced substantial proliferation in CD34++CD38 cells in the presence of IL 3 , except for kit ligand , which induced very limited proliferation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In contrast , KIT ligand ( KL ) and granulocyte macrophage colony stimulating factor ( GM CSF ) were ineffective in promoting IL 7 dependent pro B cell growth and differentiation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Erythropoiesis in myelodysplastic syndrome : expression of receptors for erythropoietin and kit ligand . ^^^ The growth factor kit ligand ( KL ) may restore the in vitro erythroid colony forming response to EPO in a subset of patients . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , also known as mast cell growth factor , kit ligand , and steel factor , is the ligand for the tyrosine kinase receptor ( SCFR ) that is encoded by the c kit proto oncogene . ^^^ Recombinant human stem cell factor ( kit ligand ) promotes human mast cell and melanocyte hyperplasia and functional activation in vivo . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
W encodes the Kit receptor tyrosine kinase , while Sl encodes the Kit ligand , Steel factor , and the receptor ligand pair are contiguously expressed at anatomical sites expected from the phenotypes of W and Sl mice . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Granulocyte / macrophage colony stimulating factor and kit ligand cotreatment did not overcome this inhibition . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In addition to its effect on megakaryocyte production and maturation , TPO has a proliferative effect on other progenitors , particularly erythroid progenitors , when used in combination with other cytokines ( erythropoietin , interleukin 3 , and kit ligand ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The effect of the mast cell growth factor ( MGF ) , also known as stem cell factor , steel factor , and kit ligand , alone or in combination with other GFs on clonogenic blast cell growth in 23 patients with acute myeloblastic leukemia ( AML ) was investigated . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
While colony forming unit erythroid ( CFU E ) and burst forming unit erythroid ( BFU E ) colonies were not present in cultures derived from EpoR / liver or yolk sac cells , hemoglobin containing BFU E colonies were detected in cultures treated with recombinant Tpo and Kit ligand or with Tpo and interleukin 3 and 11 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Combinations of cytokines , such as kit ligand ( KL ) + interleukin 3 or KL + granulocyte macrophage colony stimulating factor ( GM CSF ) upregulated the expression of CD 95 on PHPs upon in vitro culture . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
FL promotes the growth of clonogenic myeloid progenitor cells in the presence of other cytokines known to be active on myeloid progenitors , including GM CSF , interleukin 3 ( IL 3 ) , kit ligand ( KL ) , M CSF and G CSF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
All cases were also tested for their proliferative response to human FLT 3 ligand ( FL ) and KIT ligand ( KL ) and for colony formation in the presence of single or associated cytokines . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Peripheral blood blast cells were cultured in a clonogenic assay at three cell concentrations and with the following combinations of growth factors : no added growth factor ( NF ) , G CSF , GM CSF , Kit ligand ( KL ) , G CSF + KL , GM CSF + KL , and G CSF + GM CSF + KL . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We hypothesize that these staining patterns for SCF are due to either lack of staining of the receptor ligand complex or overexpression of the kit ligand in tissue that does not express the kit receptor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Purification of repopulating hemopoietic cells based on binding of biotinylated Kit ligand . ^^^ A high level of Kit expression was detectable on 1 2 % of BM cells after staining with biologically active biotinylated Kit ligand ( KL ) or with anti Kit antibodies ( ACK 2 ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Information gathered from mutant mouse models and from studies on normal puberal and adult animals points to the product of the c kit gene , a tyrosine kinase surface receptor , and the kit ligand ( KL ) as important for gametogenesis in males . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
They are now known to arise from CD34+ pluripotential stem cells , circulate through the blood as CD34+ , Fc epsilon RI , Kit+ committed but undifferentiated cells , and migrate into tissues where they mature in the presence of Kit ligand ( stem cell factor ) and other locally produced cytokines . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In addition to the T helper 2 cell mediated regulation of MMC hyperplasia during nematode infection the kit ligand , stem cell factor ( SCF ) , plays a key role in the early development of the MMC response . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Combinations of cytokines such as hepatocyte growth factor , interleukin ( IL ) 3 , IL 11 , kit ligand , GM CSF and erythropoietin were used to study the in vitro radiation dose response of human cord blood CD34+ hematopoietic progenitor cells using clonogenic survival assays . ^^^ With the combination of IL 3 , GM CSF , kit ligand and HGF , D 0 values were not significantly altered and ranged between 1 . 61 and 2 . 60 Gy . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In vitro expansion of hematopoietic progenitors and maintenance of stem cells : comparison between FLT3 / FLK 2 ligand and KIT ligand . ^^^ The effects of FLT3 / FLK 2 ligand ( FL ) and KIT ligand ( KL ) on in vitro expansion of hematopoietic stem cells were studied using lineage negative ( Lin ) Sca 1 positive ( Sca 1+ ) c kit positive ( c kit+ ) marrow cells from 5 fluorouracil ( 5 FU ) treated mice . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Compared to progenitors purified from normal mice , bone marrow progenitor cells ( lin ) from me / me mice markedly hyper proliferated in response to Kit ligand ( KL ) . stimulation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Human mast cells ( MC ) were derived from umbilical cord blood and bone marrow progenitors cultured in the presence of a conditioned medium from a human mastocytosis cell strain and recombinant human kit ligand ( rhKL ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Hormonally regulated expression and alternative splicing of kit ligand may regulate kit induced inhibition of meiosis in rat oocytes . ^^^ Mutations in the genes encoding the Kit tyrosine kinase receptor or kit ligand ( KL ) cause numerous phenotypic defects , including sterility . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We recently demonstrated a role for phosphatidylinositide 3 ' ( PI 3 ) kinase in Kit ligand ( KL ) induced adhesion of BMMCs to fibronectin . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Addition of the Kit ligand Mgf or endothelin 3 or a combination of these factors all rapidly increased the number of Dct positive cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Activation of the receptor tyrosine kinase c kit by the kit ligand , also known as stem cell factor ( SCF ) , is essential to melanocyte and germ cell development and during the early stages of hematopoiesis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
It is expressed by the primitive CD 34 positive haemopoietic stem cells and interacts with the Kit ligand for signal transduction . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Growth factors such as basic fibroblast growth factor ( bFGF ) , hepatocyte growth factor ( HGF ) and mast cell growth factor ( MGF or KIT ligand ) , and UV radiation modulate the melanogenic and mitogenic processes in vertebrate melanocytes as well . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We also determined the effect of exposure to Kit antibodies on gonocyte migration in vitro , and , conversely , asked whether addition of exogenous stem cell factor ( SCF ) , the Kit ligand , stimulates pseudopod development . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The effect of different expansion protocols on the expression levels of CD49dw / CD29 ( VLA 4 ) , CD11a / CD18 ( LFA 1 ) , CD 31 ( PECAM 1 ) , CD 44 , and CD 34 was determined after cord blood CD34+ cells were cultured for defined periods with the following : 1 ) A growth factor mix ( GFmix ) containing interleukin ( IL ) 1 , IL 3 , IL 6 , kit ligand ( KL ) , G CSF , GM CSF , and erythropoietin ( Epo ) ; 2 ) IL 3 + KL ; and 3 ) HS 5 ( a human stromal cell line supernatant ) + KL . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The ligand for c kit , known as stem cell factor , mast cell growth factor , or kit ligand , plays a central role in normal hematopoietic stem cell , melanocyte , and gametocyte development and function during embryogenesis and in adult life . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Other members of this family include Steel factor ( also known as mast cell growth factor , stem cell factor , and kit ligand ) and colony stimulating factor 1 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Although IL 10 also enhanced kit ligand ( KL ) + IL 7 induced proliferation of Lin Sca 1 ( + ) c kit+ progenitor cells , the resulting cells were predominantly myeloid progeny . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We found that anti VLA 4 treatment combined with either granulocyte colony stimulating factor ( G CSF ) treatment or kit ligand treatment leads to significant enhancement of mobilization efficiency ( fivefold to eightfold ) well above the levels produced by either cytokine alone or anti VLA 4 treatment alone . ^^^ Similar enhancement was seen when combinations of cytokines , ie , G CSF plus kit ligand or G CSF plus Flt 3 ligand were used with anti VLA 4 in primates and mice . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
This effect is however much weaker than those observed after costimulation with kit ligand or interleukin 3 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Shorter incubation periods were also found to be effective and when CD34+ cells were exposed to thrombopoietin ( TPO ) , kit ligand ( KL ) , interleukin 1alpha ( IL 1alpha ) , and IL 3 in serum free cultures for as few as 48 hours , the number of assayable CFU Meg was still increased approximately threefold over input number . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
All of the corresponding mutant KIT proteins were constitutively activated without the KIT ligand , stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The growth and development of mast cells is stimulated by mast cell growth factor , which is also known as kit ligand because its obligate receptor is KIT , the protein product of the c KIT proto oncogene . ^^^ The in vivo influence of the KIT kit ligand axis on the phenotype of human mast cells has not been determined . ^^^ We conclude that chronic stimulation of the KIT kit ligand axis does not irrevocably commit mast cells to a chymase positive or chymase negative phenotype . ^^^ These findings suggest that factors other than kit ligand predominate in determining mast cell phenotype . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Cell numbers in the suspension cultures initiated with Flt3high cells were maintained in the presence of Flt 3 ligand ( FL ) alone , and increased in response to FL plus kit ligand ( KL ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The kit ligand stem cell factor ( SCF ) significantly increased the percentage of sperm undergoing acrosome reaction . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In steel Dickie mutant mice ( Sl / Sld ) , the gene coding for the Kit ligand ( stem cell factor ) is defective . ^^^ The absence of the normal Kit ligand ( Sl / Sld mice ) had very similar effects as the absence of the Kit receptor in viable mice , mutated at the White spotting , W , locus ( W / Wv mice ) , in that neither slow waves , nor Kit receptor immunoreactivity in the region of Auerbach ' s plexus nor ICC AP were present in the small intestine . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
When BFU E growth was optimally stimulated with erythropoietin ( EpO ) + kit ligand ( KL ) the large erythroid colonies developed normally even in the absence of INS or IGF 1 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The polymerase chain reaction based differential display method ( DDRT PCR ) was used to identify mRNAs differentially expressed during the maturation of human CD34+ progenitor cells stimulated to differentiate in vitro towards granulomonocytic or erythroid lineages with a mixture of hemopoietins ( kit ligand + interleukin 3 + GM CSF in the absence or presence of erythropoietin , respectively ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Thecal cell granulosa cell interactions involve a positive feedback loop among keratinocyte growth factor , hepatocyte growth factor , and Kit ligand during ovarian follicular development . ^^^ These mesenchymal epithelial cell interactions are in part mediated by keratinocyte growth factor ( KGF ) , hepatocyte growth factor ( HGF ) , and Kit ligand ( KL ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Role of dimerization of the membrane associated growth factor kit ligand in juxtacrine signaling : the Sl17H mutation affects dimerization and stability phenotypes in hematopoiesis . ^^^ The Kit ligand ( KL ) / Kit receptor pair functions in hematopoiesis , gametogenesis , and melanogenesis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In an effort to expand human hematopoietic progenitors and stem cells in vitro , we cultured human CD 34 ( + ) c kitlow bone marrow cells in suspension in the presence of KIT ligand , FLK2 / FLT3 ligand , interleukin 6 ( IL 6 ) , and erythropoietin with or without IL 3 and tested their engrafting capabilities by injecting them into sheep fetuses . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Expression of the two major human MC growth factors , stem cell factor ( or kit ligand ) and interleukin 6 , was detected using immunohistochemistry . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In mouse bone marrow derived mast cells , the kit ligand selectively up regulates GTP cyclohydrolase 1 activity ( Ziegler , I . , Hltner , L . , Egger , D . , Kempkes , B . , Mailhammer , R . , Gillis , S . , and Rdl , W . ( 1993 ) J . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Finally , the proliferation of the neurofibrosarcoma derived Schwann cells can be increased by stimulation with Kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
FL acts synergistically in vitro with a number of other hematopoietic growth factors such as IL 3 , IL 6 , IL 11 , IL 12 , KIT Ligand and GM CSF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Consequences of exclusive expression in vivo of Kit ligand lacking the major proteolytic cleavage site . ^^^ The membrane growth factor Kit ligand ( KL ) , the ligand of the Kit receptor tyrosine kinase , is encoded at the Sl locus , and mice carrying Sl mutations have defects in hematopoiesis , gametogenesis , and melanogenesis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
These cells were cultured with either the HS 5 stromal cell line serum deprived conditioned media supplemented with 10 ng / mL kit ligand ( HS 5CM / KL ) or a recombinant growth factor mix ( GFmix ) containing 10 ng / mL each of interleukin ( IL ) 1 , IL 3 , IL 6 , KL , granulocyte colony stimulating factor , granulocyte macrophage colony stimulating factor , and 3 U / mL of erythropoietin . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In CD34+ selected cells , preincubation with amifostine increased formation of CFU GEMM up to 38 fold and produced macroscopic colonies , exceeding colony number in cultures initiated with optimal concentrations of interleukin 1 ( IL 1 ) , IL 3 , or kit ligand ( KL ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Using the adhesive interaction of a cytokine dependent human hemopoietic cell line to immobilized fibronectin , we have analyzed the requirements in divalent cations Mn2+ , Mg2+ and Ca2+ for alpha 4 beta 1 and alpha 5 beta 1 activation by `` inside out ' ' mechanisms triggered by cytokines such as granulocyte macrophage colony stimulating factor or KIT ligand , or by external conformational constraints with the function activating anti beta 1 integrin monoclonal antibody 8A2 . ^^^ The presence of the absence of these divalent cations did not inhibit early tyrosine phosphorylation induced by the binding of KIT ligand to its tyrosine kinase receptor KIT . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Analysis of BMMC expressing wild type and dominant negative mutant forms of Rac 1 and JNK revealed that the Rac1 / JNK pathway is critical for Kit ligand ( KL ) induced proliferation of mast cells but not for suppression of apoptosis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Both death signals ( TNT alpha , Fas ligand ) and survival signals ( LIF , kit ligand ) are present in the embryonic gonad . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
It was suggested that several murine genes , including Zfx , c = kit , and the kit ligand , should be fertile candidates for investigation of the etiology of POF in human families . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Recent work shows that they can also contribute significantly to the expression of innate immunity ; furthermore , survival in a model of acute bacterial infection that is dependent on complement and mast cells can be greatly enhanced by long term treatment of mice with the kit ligand ( stem cell factor ) at least in part because of the effects of such treatment on mast cell numbers and / or function . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mast cell expression of gelatinases A and B is regulated by kit ligand and TGF beta . ^^^ Progelatinase B mRNA and enzyme expression are strongly induced by the critical mast cell growth factor , kit ligand , which is produced by fibroblasts and other stromal cells . ^^^ Induction of progelatinase B is blocked by U 73122 , Ro 31 8220 , and thapsigargin , implicating phospholipase C , protein kinase C , and Ca2+ , respectively , in the kit ligand effect . ^^^ The profibrotic cytokine TGF beta virtually abolishes the gelatinase B mRNA signal and also attenuates kit ligand mediated induction of gelatinase B expression , suggesting that an excess of TGF beta in inflamed or injured tissues may alter mast cell expression of gelatinase B , which is implicated in extracellular matrix degradation , angiogenesis , and apoptosis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In amniotes , Steel encodes the cytokine Steel factor ( mast cell growth factor ; stem cell factor ; kit ligand ) , which is expressed along the migratory pathways of melanocyte precursors and is required by these cells for their migration and survival ; mammalian Steel mutants resemble white mutant axolotls in having a deficit or complete absence of pigment cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The major findings of our studies are as follows : 1 ) There are no detectable signals around GDF 9 deficient follicles for several theca cell layer markers [ i . e . 17alpha hydroxylase , LH receptor ( LHR ) , and c kit , the receptor for kit ligand ] . ^^^ Up regulation of kit ligand , via signaling through c kit on the oocyte , may be directly involved in the increased size of GDF 9 deficient oocytes and the eventual demise of the oocyte ; 3 ) After loss of the oocyte , the cells of the GDF 9 deficient follicles remain in a steroidogenic cluster that histologically resembles small corpora lutea . ^^^ This demonstrates that in the absence of GDF 9 , the follicles are incompetent to emit a signal that recruits theca cell precursors to surround the follicle ; 2 ) The primary follicles of GDF 9 deficient mice demonstrate an up regulation of kit ligand and inhibin alpha . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Compared with interleukin 3 ( IL 3 ) , IL 6 , and leukemia inhibitory factor ( LIF ) , the combination of thrombopoietin ( TPO ) , Flt 3 ligand ( FL ) , and Kit ligand ( KL ) effected a 73 fold increase in NGFR expression among CD34+ cells ( to 14 % ) and a 14 fold increase in NGFR expression among total cells ( to 10 % ) after LTC . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Transforming growth factor beta regulates Kit ligand expression in rat ovarian surface epithelial cells . ^^^ Kit tyrosine kinase receptors and their ligand , kit ligand ( KL ) are expressed within ovarian follicles , and ligand induced receptor activation appears to account for some of the cell cell interactions important for oocyte development . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Candidate fetal HSCs exhibited a dose dependent proliferative response to CH 11 as well as to sCD95L when combined with kit ligand ( KL ) + interleukin 3 ( IL 3 ) under serum deprived culture conditions . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , also known as c kit or kit ligand , induces multiple effects on mast cells , including proliferation , differentiation , maturation , and prevents apoptosis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Saturation binding analysis indicated that the isoforms do not differ significantly in their affinity for the KIT ligand , Steel Factor ( SLF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Oocyte regulation of kit ligand expression in mouse ovarian follicles . ^^^ Kit ligand ( KL ) , a product of granulosa cells in ovarian follicles , is a putative regulator of oocyte development . ^^^ The results therefore support the hypothesis that oocytes regulate granulosa cell kit ligand mRNA levels in a way that is characteristic of the stage of growth and development of the oocyte . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Kit ligand actions on ovarian stromal cells : effects on theca cell recruitment and steroid production . ^^^ Granulosa cells have been shown to produce kit ligand / stem cell factor ( KL ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Recent work shows that mast cells can also participate in innate immunity to bacterial infection and that the expression of such mast cell dependent natural immunity can be significantly enhanced by long term treatment of mice with the kit ligand , stem cell factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Medium for the ex vivo cultures contained either serum and six recombinant human hematopoietic growth factors ( GFs ) , including Flt 3 ligand ( FL ) , Kit ligand ( KL = stem cell factor ) , thrombopoietin ( Tpo ) , interleukin 3 ( IL 3 ) , granulocyte colony stimulating factor ( G CSF ) , and interleukin 6 ( IL 6 ) , or a serum free medium containing only FL , KL , and Tpo . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
CD34+ cells from a total of 93 patients with either acute myeloid leukemia ( AML ; n = 25 ) , chronic myeloid leukemia ( CML ; n = 21 ) , chronic lymphocytic leukemia ( CLL ; n = 18 ) , polycythemia vera ( PV ; n = 16 ) , or myelodysplastic syndromes ( MDS ; n = 13 ) were analyzed before and in 19 patients after ex vivo expansion in the presence of multiple cytokines ( kit ligand , interleukin 3 , interleukin 6 , and granulocyte colony stimulating factor plus erythropoietin ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Development of the interstitial cell of Cajal : origin , kit dependence and neuronal and nonneuronal sources of kit ligand . ^^^ The roles of neural crest derived cells , neurons , Kit , and Kit ligand ( KL ) in ICC development were analyzed . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Expression of c kit and kit ligand in benign and malignant prostatic tissues . ^^^ The tyrosine kinase receptor c kit and its ligand [ kit ligand ( KL ) or stem cell factor ( SCF ) ] exert a broad range of biological activities during organogenesis and normal cell development . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The current study investigates the potential role of the growth factor kit ligand ( KL ) / stem cell growth factor and its receptor c kit in normal OSE biology and ovarian cancer . ^^^ Expression and action of kit ligand / stem cell factor in normal human and bovine ovarian surface epithelium and ovarian cancer . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Murine bone marrow derived mast cells as potent producers of IL 9 : costimulatory function of IL 10 and kit ligand in the presence of IL 1 . ^^^ Herein we show that a dramatic increase of IL 9 production is observed when BMMC activated with ionomycin / IL 1 or with IgE Ag complexes / IL 1 are treated with either additional kit ligand ( KL ) or IL 10 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The CFU C content could be expanded 50 fold by culture for 1 week in serum deprived medium and the growth factors kit ligand and granulocyte macrophage colony stimulating factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
While unstimulated CD34+ cells expressed VEGF A mRNA weakly without cytokine release in vitro , incubation for 24 hours with a single cytokine ( e . g . , kit ligand [ KL ] ) resulted in increased VEGF A mRNA expression and significant secretion of VEGF A into the supernatant . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
It was found Il 6 causes approximately 35 % inhibition of early BFU E growth stimulated with erythropoietin , kit ligand and interleukin 3 , Il 6 did not inhibit erythroid colony formation by late BFU E and CFU E progenitors . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Effects of Kit Ligand and anti Kit antibody on growth of cultured mouse preantral follicles . ^^^ Evidence obtained from genetic mutants as well as in vivo experiments suggest that Kit and Kit Ligand ( KL ) may regulate early follicular morphogenesis and function . ^^^ Two concentrations of Kit Ligand ( 20 and 50 ng / ml ) or an antibody blocking the Kit Kit Ligand interactions ( SC 1494 ) were added to preantral follicles grown individually for 12 days and which were finally triggered to ovulate . ^^^ Blockade of Kit Kit Ligand interactions by addition of a blocking antibody decreased oocyte survival 6 9 days after addition of the antibody , but did not upset granulosa cell proliferation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
A number of studies have demonstrated that the pituitary hormones follicle stimulating hormone ( FSH ) and luteinizing hormone ( LH ) as well as granulosa cell derived growth factors such as activins , inhibins , and kit ligand are necessary for follicle development . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The control of oocyte apoptosis is not fully understood , although survival factors ( LIF , kit ligand and FGF ) , as well as death inducing factors ( fas ligand , TGFbeta ) , have been identified . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
MGF ( KIT ligand ) is a chemokinetic factor for melanoblast migration into hair follicles . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Among the cytokine combinations tested , kit ligand ( KL ) and IL 15 provided the best conditions for generating CD 56 ( + ) NK cells from CD 38 ( + ) CD 34 ( ++ ) Lin ( ) cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Roles of KIT and KIT LIGAND in ovarian function . ^^^ Evidence from mouse mutants indicates that the Kit gene encoding KIT , a receptor present on the oocyte and theca cells , and the Mgf gene encoding KIT LIGAND , the ligand of KIT , are important regulators of oogenesis and folliculogenesis . ^^^ Recently , in vitro cultures of fetal gonads , of follicles and of oocytes have identified specific targets for the KIT KIT LIGAND interaction . ^^^ In fetal gonads , an anti apoptotic effect of KIT KIT LIGAND interactions on primordial germ cells , oogonia and oocytes has been demonstrated . ^^^ In postnatal ovaries , the initiation of follicular growth from the primordial pool and progression beyond the primary follicle stage appear to involve KIT KIT LIGAND interactions . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In addition , TPO directly and potently synergizes with other early acting cytokines ( kit ligand , flt 3 ligand and interleukin 3 ) to promote multilineage growth of the same progenitor cell population . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stimulation of mouse bone marrow cells with kit ligand , FLT 3 ligand , and thrombopoietin leads to efficient retrovirus mediated gene transfer to stem cells , whereas interleukin 3 and interleukin 11 reduce transduction of short and long term repopulating cells . ^^^ Large quantitative differences in reconstitution were observed after transplantation with graded numbers ( 1000 30 , 000 ) of EGFP ( + ) cells preconditioned with various combinations of Kit ligand ( KL ) , FLT 3 ligand ( FL ) , thrombopoietin ( TPO ) , interleukin 3 ( IL 3 ) , and IL 11 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Comparison of recombinant growth differentiation factor 9 and oocyte regulation of KIT ligand messenger ribonucleic acid expression in mouse ovarian follicles . ^^^ KIT ligand ( KL ) mRNA expression in granulosa cells is thought to be regulated by oocytes ; however , the factor ( s ) that mediate this effect are not known . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We also find that transgenic , but not wild type cells , survive and proliferate rapidly in response to steel factor ( Kit ligand ) . ^^^ These studies show that two separate signals are necessary to prevent apoptosis in HSCs , and that Kit ligand by itself provides a strong proliferative stimulus to HSCs . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Detection and quantitation of stem cell factor ( kit ligand ) in the synovial fluid of patients with rheumatic disease . ^^^ OBJECTIVE : To identify and quantitate stem cell factor ( SCF ; kit ligand ) in the serum and synovial fluid ( SF ) of patients with rheumatoid arthritis ( RA ) and to compare these values with those measured in normal serum , RA serum , SF of patients with other rheumatic diseases , and conditioned medium from cultured synoviocytes . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Both FSH and hCG stimulated steady state levels of keratinocyte growth factor ( KGF ) , hepatocyte growth factor ( HGF ) , and kit ligand ( KL ) mRNA in OSE cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Using the pre colony forming unit ( pre CFU ) assay as a model for early myelopoiesis , we compared the effects of serum containing and serum free media and evaluated different cytokine cocktails [ interleukin ( IL ) 1 , IL 3 , IL 6 , kit ligand with and without the Flt3 / Flk2 ligand ( FL ) ] . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Kit ligand ( KL ) also strongly synergized with EPO in stimulating the growth of these BFU E . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
It is well established that human mast cell proliferation and maturation are regulated by kit ligand ( stem cell factor ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The type 3 tyrosine kinase receptor c KIT and its ligand stem cell factor ( SCF ; also known as KIT ligand , mast cell growth factor and steel factor ) are closely involved in the regulation of a wide range of tissues at different stages of life . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Steel factor ( SF ) or Kit ligand is produced as a membrane bound form ( mSF ) and a soluble form . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Candidate stem cells were transduced immediately after isolation or after up to 4 days of culture in serum deprived medium containing the growth factors kit ligand and granulocyte macrophage colony stimulating factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Indeed , upon treatment with the KIT ligand ( KITL ) , the extracellular signal regulated protein kinase was phosphorylated , and the expression of early responsive genes was induced . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In this paper we show the megakaryocytopoietic productivity of small scale in vitro serum free cultures of human CD34+ cells containing MK growth and development factor ( MGDF ) and stem cell factor ( Kit ligand ; SCF ) + / granulocyte colony stimulating factor ( G CSF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The KIT receptor , present on oocyte and theca cells in ovarian follicles , and its ligand , KIT LIGAND , produced by granulosa cells , are encoded at the Kit gene and the Mgf gene , respectively . ^^^ Firstly , the amounts of KIT and KIT LIGAND proteins in the ovaries of mice at different ages were determined . ^^^ Western blotting showed that the amounts of both KIT and KIT LIGAND proteins were decreased in mutant mice . ^^^ It is concluded that the Kit ( W lacZ ) mutation affects the expression of KIT and KIT LIGAND proteins , resulting in alterations in granulosa cell proliferation and / or oocyte growth in preantral follicles . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The Kit ligand derived from M . spretus showed two amino acid changes in the extracellular domain compared with that from C57BL and it appears that the ligand receptor interaction between C57BL and SPR may influence the male hybrid sterility of KitW / KitS . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
CD 38 ( ) and CD 38 ( + ) progenitors that expressed high levels of CD 34 were grown in serum deprived medium supplemented with kit ligand , flk2 / flt3 ligand , GM CSF , c mpl ligand , erythropoietin , and IL 15 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The aim of this study was to identify signal transduction pathways activated by erythropoietin ( EpO ) and erythropoietin co stimulatory factors ( kit ligand ) , insulin like growth factor , thrombopoietin , interleukin 3 and granulocyte macrophage colony stimulating factor ) in normal human bone marrow CD 34 ( + ) cells and d 11 erythroid burst forming unit derived glycophorin+ cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mast cell development depends on stem cell factor ( Kit ligand ) and its receptor , c Kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Human CD34+ cells from CB or PBSC were cultured in serum free medium , with or without hematopoietic growth factors ( FKT : FLT 3 ligand [ FL ] , KIT ligand [ KL ] , and thrombopoietin [ TPO ] ) , and with or without soluble Fas ligand ( sFasL ) or agonistic anti Fas antibody . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
However , LIF treatment did increase expression of Kit ligand ( KL ) mRNA in cultured granulosa cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Strain dependent variation existed among C57BL / 6 ( B 6 ) , DBA / 2 , and BXD recombinant inbred mice in the responsiveness of primitive progenitor cells to the early acting cytokines kit ligand , flt 3 ligand , and thrombopoietin . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Incubation with the flt 3 or Kit ligand induced the expression of unilineage HGF receptors , allowing these cells to respond to their respective ligands . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
A negative feedback system between oocyte bone morphogenetic protein 15 and granulosa cell kit ligand : its role in regulating granulosa cell mitosis . ^^^ Herein we present a previously undescribed oocyte granulosa cell ( GC ) feedback communication system involving an oocyte derived factor , bone morphogenetic protein 15 ( BMP 15 ) and a GC derived factor , kit ligand ( KL ) , both of which have been shown to be crucial regulators of female reproduction . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Infiltrating Y chromosome positive MC were detected by fluorescence in situ hybridization . mRNA expression of interleukin 3 ( IL 3 ) and of the two differentially spliced isoforms of kit ligand ( KL , stem cell factor ) was quantified using reverse transcription polymerase chain reaction . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Previous experiments have shown that kit ligand ( KL ) , basic fibroblast growth factor ( bFGF ) and leukemia inhibitory factor ( LIF ) promote the primordial to primary follicle transition . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
METHODS : CD34+ cell preparations purified from CB or PBSC were cultured in serum free medium containing FKT : FLT 3 ligand ( FL ) , KIT ligand ( KL ) , and thrombopoietin ( TPO ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The late phase of hematopoietic recovery was driven by PlGF induced upregulation of matrix metalloproteinase 9 , mediating the release of soluble Kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Interstitial cells of Cajal that carry the tyrosine kinase receptor ( c Kit ) develop as either myenteric ICCs or muscular ICCs under the influence of the kit ligand , which can be provided by neuronal and nonneuronal cells , for example , smooth muscle cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
GDF 9 treatment increased kit ligand ( KL ) mRNA expression in bovine granulosa cells after 2 days of culture . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Sorted NBM CD 34 ( + ) CD 133 ( + ) and CD 34 ( + ) CD 133 ( ) subsets were evaluated for Mk clonogenic capacity before and after in vitro proliferation in serum free liquid culture containing kit ligand , Flt 3 ligand , thrombopoietin , interleukin 3 , and interleukin 6 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
An allelic series of mutations in the kit ligand gene of mice . 1 . ^^^ The product of the Sl locus is Kit ligand ( or Kitl ; also known as mast cell growth factor , stem cell factor , and Steel factor ) , which is a member of the helical cytokine superfamily and is the ligand for the Kit receptor tyrosine kinase . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
An allelic series of mutations in the Kit ligand gene of mice . 2 . ^^^ The ligand for the Kit receptor tyrosine kinase is Kit ligand ( Kitl ; also known as mast cell growth factor , stem cell factor , and Steel factor ) , which is encoded at the Steel ( Sl ) locus of mice . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Kit ligand ( KitL ) is the most important growth factor for development of mast cells , and the decrease of skin mast cells in B 6 mi / mi mice was attributable to the reduced expression of c kit receptor tyrosine kinase ( KIT ) that is a receptor for KitL . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We have previously demonstrated that BMP 15 regulates granulosa cell ( GC ) proliferation and differentiation ; namely , BMP 15 promotes GC mitosis , suppresses follicle stimulating hormone ( FSH ) receptor expression , and stimulates kit ligand expression . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
HbF reactivation in sibling BFU E colonies : synergistic interaction of kit ligand with low dose dexamethasone . ^^^ We suggested that a key role is played by kit ligand ( KL ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Megakaryocytes generated by ex vivo expansion of hematopoietic progenitor cells with kit ligand and thrombopoietin were analyzed for expression of FGF 2 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Atm , kit ligand and its receptor c kit are necessary for the maintenance of ovarian follicle pool . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We evaluated the effects of the tyrosine kinase inhibitors STI 571 and AG 1296 on BaF 3 cells expressing wild type KIT ( KIT ( WT ) ) or activating mutants of KIT ( KIT ( G 559 ) and KIT ( V 814 ) ) in the presence or absence of the KIT ligand , stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Direct evidence that Bcr Abl tyrosine kinase activity disrupts normal synergistic interactions between Kit ligand and cytokines in primary primitive progenitor cells . ^^^ We previously reported that chronic myelogenous leukemia ( CML ) primitive granulocyte monocyte ( GM ) progenitors have a greatly reduced requirement for kit ligand ( KL ) to achieve optimal growth with granulocyte colony stimulating factor ( G CSF ) + granulocyte monocyte colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Kit ligand ( Kitl ) , which is a member of the helical cytokine superfamily , is encoded by the Steel ( Sl ) locus of mice and is essential for the development of hematopoietic cells , germ cells , and melanocytes . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The mobilization of stem cells from marrow is a dynamic process , regulated by shear stress imparted by blood flow , and the activation of metalloproteinases that induce the release of ' Kit ligand ' , facilitating egress from the marrow to the circulation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Inactivation of the forkhead transcription factor FoxO 3 is essential for PKB mediated survival of hematopoietic progenitor cells by kit ligand . ^^^ OBJECTIVE : Kit ligand ( KL ) is a major survival factor for hematopoietic stem cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The role of Kit ligand in melanocyte development and epidermal homeostasis . ^^^ Kit ligand ( Kitl ) also known as steel factor , stem cell factor and mast cell growth factor plays a crucial role in the development and maintenance of the melanocyte lineage in adult skin . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Kit ligand and basic fibroblast growth factor expression was unchanged , but TGFalpha expression was decreased in whole ovaries . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Kit ligand ( Kitl ) , encoded by the Steel ( Sl ) locus , plays an essential role in hematopoiesis , gametogenesis , and melanogenesis during both embryonic and adult life . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Cyclic adenosine monophosphate ( cAMP ) stimulation of the kit ligand promoter in sertoli cells requires an Sp 1 binding region , a canonical TATA box , and a cAMP induced factor binding to an immediately downstream GC rich element . ^^^ Expression of Kit ligand ( KL ) mRNA is induced in primary prepuberal Sertoli cells by FSH and by other agents that increase cAMP levels . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Phosphatidylinositol 3 kinase is essential for kit ligand mediated survival , whereas interleukin 3 and flt 3 ligand induce expression of antiapoptotic Bcl 2 family genes . ^^^ Cytokines such as interleukin 3 ( IL 3 ) , kit ligand ( KL ) , and flt 3 ligand ( FL ) promote survival of hematopoietic stem cells and myeloid progenitor cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In this study , quiescent bone marrow derived CD34+ erythroid burst forming units ( BFU E ) were found to be resistant to the inhibitory effects of tumour necrosis factor ( TNF ) alpha and beta as well as interferon ( IFN ) alpha , beta and gamma , in contrast to those stimulated by a combination of erytrhropoietin ( Epo ) plus kit ligand ( KL ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Role of c kit / Kit ligand signaling in regulating vasculogenesis . ^^^ We will give evidence that matrix metallo proteinase 9 mediated Kit ligand ( Stem cell factor ) processing is essential for cell mobilization induced by chemo / cytokines , like vascular endothelial growth factor ( VEGF ) , Placental growth factor ( PlGF ) , stromal cell derived factor 1 ( SDF 1 ) . ^^^ These studies may provide the basis for the development of new therapeutic strategies for vascular diseases through targeting kit ligand mediated mobilization and homing of bone marrow derived progenitor cells for cell therapy and cancer therapy . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Here , we show that homozygous Nf 1 mutant ( Nf 1 / ) Schwann cells secrete Kit ligand ( KitL ) , which stimulates mast cell migration , and that Nf1+ / mast cells are hypermotile in response to KitL . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Sixteen routinely processed IFP specimens ( 14 gastric , 1 ileal , and 1 rectal ) were immunohistochemically stained for antibodies to CD 34 , HMB 45 , desmin , smooth muscle actin , calponin , h caldesmon , anaplastic lymphoma kinase , S 100 protein , epithelial membrane antigen , c kit ( CD 117 ) , stem cell factor ( SCF / N19 or kit ligand ) , p 53 , bcl 2 , cyclin D 1 , and human herpesvirus 8 ( HHV 8 ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Microarray expression analyses , together with independent quantitative real time reverse transcriptase polymerase chain reaction results , indicate that HMGA1a regulates genes involved in the Ras extracellular signal related kinase ( Ras / ERK ) mitogenic signaling pathway , including KIT ligand and caveolins 1 and 2 . ^^^ Cholesterol depletion , decreased caveolin , and increased KIT ligand expression , are all independently associated with the activation of Ras / ERK signaling . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Genetic analysis of Kit ligand functions during mouse spermatogenesis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
These results indicate that the theca recruitment can occur independently of GDF 9 , but the differentiation of thecal cells is blocked ; and 4 ) . inhibin / activin subunits betaA , betaB , and Kit ligand ( Kitl ) mRNA are highly up regulated , suggesting that the increased activins and KITL play functional roles in early folliculogenesis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Kit ligand and basic fibroblast growth factor interactions in the induction of ovarian primordial to primary follicle transition . ^^^ The objective of the current study is to investigate interactions between kit ligand ( KL ) ( i . e . stem cell factor ) and basic fibroblast growth factor ( bFGF ) that promote the primordial to primary follicle transition in rat ovaries . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Serum KIT and KIT ligand levels in patients with gastrointestinal stromal tumors treated with imatinib . ^^^ We monitored the serum levels of KIT , KIT ligand ( stem cell factor , SCF ) , and the vascular endothelial growth factor ( VEGF ) in patients with advanced GISTs treated with imatinib in a prospective randomized trial . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
These Ednrb wild type neural tubes need not be capable of generating melanocytes themselves , but must be capable of providing KIT ligand , the cognate ligand for the tyrosine kinase receptor KIT . ^^^ In fact , soluble KIT ligand is sufficient to induce tyrosinase expression in Ednrb deficient cultures . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In human mast cells , derived from CD 34 ( + ) peripheral blood cells , we observed that Kit ligand ( KL ) failed to induce degranulation but acted in synergy with antigen to markedly enhance degranulation , levels of cytokine gene transcripts , and production of cytokines . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
After 4 day culture period , number of follicles was counted and the expression of transcripts for FSH receptor ( FSHR ) , kit ligand ( KL ) , and c kit was measured by RT PCR . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
KIT ligand ( KL ) and its receptor , c kit , are coexpressed in many types of cancer cells and have been implicated in tumor growth and angiogenesis . ^^^ Human KIT ligand promoter is positively regulated by HMGA 1 in breast and ovarian cancer cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Interaction between BMMCs and the mesentery was examined by using mast cell deficient WBB6F1 ( F 1 ) W / Wv [ c kit receptor tyrosine kinase ( KIT ) mutant ] , F 1 Sl / Sld [ KIT ligand stem cell factor mutant ] , and F 1 tg / tg [ a practically microphthalmia transcription factor ( MITF ) null mutant ] mice . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor , also known as Kit ligand ( Kitl ) , belongs to the family of dimeric transmembrane growth factors . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mast cell differentiation and activation are regulated , respectively , by stem cell factor ( SCF ; also known as Kit ligand ) and by allergen in complex with allergen specific immunoglobulin E ( IgE ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The aims of this study were : 1 ) to determine the effect of FSH on expression of Kit ligand ( KL ) , growth / differentiation factor 9 , bone morphogenetic protein ( BMP ) 15 , and Kit during growth of oocyte granulosa cell complexes ( OGCs ) in vitro ; 2 ) to investigate the role of BMP 15 in regulation of KL expression ; and 3 ) to correlate mRNA expression with oocyte growth . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
This is apparent in mice lacking connexin 43 ( Cx 43 , a gap junction protein strongly expressed in granulosa cells ) , or growth / differentiation factor 9 ( GDF 9 , an oocyte specific growth factor that stimulates granulosa cell proliferation and differentiation ) , or in mice expressing a mutant form of Kit ligand ( KITL , a paracrine factor that , in the ovary , is secreted by granulosa cells to stimulate oocyte growth ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , another alternative name is kit ligand , is essential for the development of early follicles . ^^^ By using cultured ovaries that are rich in primordial follicles , the action of SCF ( kit ligand ) on early follicular development and the activated signal transduction pathways were investigated . ^^^ SCF ( kit ligand ) promoted early follicle development . ^^^ PKC and MEK but not PKA were involved in the signal transduction of SCF ( kit ligand ) as indicated by results using their specific pharmacological inhibitors . ^^^ SCF ( kit ligand ) also enhanced the phosphorylation of two MEK substrates , Erk 1 and 2 ( Erk1 / 2 ) in thecal interstitial cells where PKC might play an important role indicated by results using its inhibitors . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Since the cloning of the Kit ligand in the early 1990s , there has been an explosion of information relating to the mechanism of action of normal forms of Kit as well as activated mutants . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Further , K Ras ( / ) hematopoietic progenitors had reduced proliferation in response to erythropoietin and Kit ligand compared with control cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
LN stroma can provide key signals for T cell development including IL 7 , Kit ligand , and Delta like 1 but lacks Wnt 4 and Wnt7b transcripts . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Leydig cell tumors express platelet derived growth factor ( PDGF ) , kit ligand and their respective receptors , PDGFR and c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
KIT ligand ( KITL ) and EDN 3 worked synergistically to induce melanocyte differentiation in vitro ; however , the complete lack of EDNRB signaling attained by the use of EDN 3 / ES cells and an EDNRB antagonist , BQ 788 , revealed that the resulting failure of melanocyte development was not compensated by the further activation of KIT signaling by adding KITL . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NCCmelb4M5 cells do not express Kit and are immortal and stable in the absence of Kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
It is well established that Kit ligand ( KITL , also known as stem cell factor and mast cell growth factor ) is required for the survival and proliferation of PGCs . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Major differences in the constitutively activation of Akt / PKB , MAP kinases and STATs pathways were observed between KIT ( K641E ) and KIT ( del 559 ) , whereas KIT ligand elicited responses in both mutants . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The mesenchymal growth factor keratinocyte growth factor ( KGF ) ( i . e . , fibroblast growth factor 7 ) and the epithelial growth factor kit ligand ( KITL ) are known to interact to coordinate the growth of later stage antral follicles . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Locally produced factors that promote the primordial to primary follicle transition include growth factors such as kit ligand ( KL ) , leukaemia inhibitory factor ( LIF ) , bone morphogenic proteins ( BMP ' s ) , keratinocyte growth factor ( KGF ) and basic fibroblast growth factor ( bFGF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In Experiment 3 , the effect of KIT ligand ( KL ) at 0 ng / ml , 10 ng / ml and 100 ng / ml , on lectin aggregated primordial follicles cultured on non coated wells was tested . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Finally , purified p85alpha / p85beta+ / c kit+ cells had a decrease in proliferation in response to kit ligand ( kitL ) , a growth factor important for controlling HSC function in vivo . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Kit ligand ( KL ; stem cell factor ) is produced by mesenchymal cells , and in the liver by biliary epithelial cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
CD34+CD38 and CD34+CD38+ cells from umbilical cord blood were analyzed before and after culture with thrombopoietin ( TPO ) , FLT 3 ligand ( FL ) and kit ligand ( KL ; or stem cell factor ) in different combinations : TPO + FL + KL , TPO + FL and TPO , at concentrations of 50 ng / mL each . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We studied the effect of two different cytokine combinations kit ligand ( KL ) , interleukin 3 ( IL 3 ) , and Flt 3 ligand supplemented with thrombopoietin and IL 11 ( combination 1 ) or granulocyte / macrophage colony stimulating factor ( GM CSF ) and G CSF ( combination 2 ) for their ability to affect speed and extent of engraftment using limited numbers ( 5 10 10 ( 4 ) ) of murine bone marrow ( BM ) light density ( LD ) cells or their progeny expanded ex vivo in the presence one or the other cytokine combination for 6 days . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
CONCLUSION : Using microarray analysis we show that elevated levels of Nurr 1 leads to increased gene expression of pro inflammatory genes : IL 8 , Amphiregulin and Kit ligand in a model cell line . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Functional studies show that treatment of CD34+ progenitors with miR 221 and 222 , via oligonucleotide transfection or lentiviral vector infection , causes impaired proliferation and accelerated differentiation of E cells , coupled with down modulation of kit protein : this phenomenon , observed in E culture releasing endogenous kit ligand , is magnified in E culture supplemented with kit ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
BACKGROUND : CD 117 , the receptor for kit ligand , which is a growth factor for melanocyte migration and proliferation , has shown differential staining in various benign and malignant melanocytic lesions . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The Kit ligand SCF or stem cell factor ( SCF ) is a multipotent growth factor , acting as an important growth factor for human mast cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Coexpression of Kit ligand and c kit has been reported in some gynecologic tumors . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Plasma concentrations of haematopoietic growth factors ( FMS like tyrosine kinase 3 ( Flt 3 ) ligand , kit ligand ) , markers of hypoxia ( vascular endothelial growth factor ( VEGF ) ) and stimulators of angiogenesis ( VEGF , hepatocyte growth factor ( HGF ) ) and markers of systemic inflammation ( tumour necrosis factor ( TNF ) alpha , interleukin ( IL ) 6 , IL 8 ) were measured . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Oocyte granulosa cell interactions during mouse follicular development : regulation of kit ligand expression and its role in oocyte growth . ^^^ Particular emphasis is given to granulosa cell derived Kit Ligand ( KitL ) , whose functional importance for oocyte growth has been demonstrated by a wide range of in vivo and in vitro studies . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Protein and mRNA expression of activin A , follistatin , Kit ligand ( KL ) , growth differentiation factor 9 ( GDF 9 ) and bone morphogenetic protein 15 ( BMP 15 ) in non cultured and cultured follicles were studied by immunohistochemistry and PCR . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The human KIT ligand gene , known also as human stem cell factor , is the ligand of the c kit transmembrane tyrosine kinase receptor ( KIT ) . ^^^ Investigation of the human stem cell factor KIT ligand gene , KITLG , in women with 46 , 20 spontaneous premature ovarian failure . ^^^ OBJECTIVE : To investigate mutations in the human KIT ligand gene ( KITLG ) gene as a mechanism of 46 , 20 spontaneous premature ovarian failure . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Ovaries from 4 day old rats were placed into culture and treated for 2 weeks with platelet derived growth factor ( PDGF ) , anti PDGF neutralizing antibody , vascular endothelial growth factor ( VEGF ) , neuregulin ( NRG ) , or kit ligand ( KITL ) as a positive control . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In addition they responded to factors that regulate normal erythroid development , namely erythropoietin ( Epo ) , interleukin 3 ( IL 3 ) , and mast cell growth factor ( MGF ) , the ligand to the c kit encoded receptor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
They are fibroblast growth factor ( FGF ) , hepatocyte growth factor ( HGF ) , and mast cell growth factor ( MGF ) , which activate the FGF receptor , c Met , and c Kit , respectively , known to be receptor tyrosine kinases . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We have previously reported the identification of a novel mast cell growth factor ( MGF ) that was shown to be a ligand for c kit and is encoded by a gene that maps near the steel locus on mouse chromosome 10 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mast cell growth factor , a ligand for the receptor encoded by c kit , affects the growth in culture of the blast cells of acute myeloblastic leukemia . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The line grows slowly without stimulation but responds to interleukin 3 ( IL 3 ) , GM CSF and mast cell growth factor ( MGF ) , a ligand for the receptor encoded by the c kit oncogene . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The c kit proto oncogene encodes the receptor for a novel hemopoietic cytokine , termed stem cell factor ( SCF ) or mast cell growth factor ( MGF ) according to its stimulating spectrum . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The cell surface molecule encoded by the protooncogene c kit has recently been identified as the receptor for a growth factor variously termed stem cell factor ( SCF ) , mast cell growth factor or steel factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Differential expression of mast cell growth factor receptor ( c kit ) by peritoneal connective tissue type mast cells and tissue culture derived mast cells . ^^^ Flow cytometric analysis using biotinylated recombinant murine mast cell growth factor ( rMuMGF ) showed that 1 to 3 % of the cells in the peritoneal cavity exhibited MGF receptor ( MGFR ) ( or c kit ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The mast cell growth factor ( MGF ) affects migration , proliferation and differentiation of erythroid and myeloid progenitor cells by binding to a transmembrane receptor tyrosine kinase encoded by the c Kit proto oncogene . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Growth and differentiation of normal mast cells is dependent on mast cell growth factor ( MGF ) , the ligand of the c kit protooncogene product . ^^^ Kit ligand / mast cell growth factor independent differentiation of mast cells in myelodysplasia and chronic myeloid leukemic blast crisis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mast cell growth factor ( MGF ) , a molecule that serves as a ligand for the receptor tyrosine kinase c kit , is important in mast cell differentiation , migration , and activation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Human MC progenitors can be detected in the bone marrow as well as in the peripheral blood ( pb ) and are responsive to the mast cell growth factor SCF , the ligand of the c kit tyrosine kinase receptor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Expression of the mast cell growth factor ( MGF ) receptor , c kit ( CD 117 ) , was also demonstrable , whereas the skin mast cell marker C5aR ( CD 88 ) could not be detected on mast cell precursors . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
These cells can be classified as basophils or mast cells using monoclonal antibodies ( mAbs ) against leukocyte antigens , including mast cell tryptase , c kit ( = mast cell growth factor [ MGF ] receptor ) , interleukin 3 receptor alpha chain ( IL 3R alpha = CD 123 ) , and CD11b ( C3biR ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The molecular studies have also enumerated several possible differentiation controls such as switching of KIT and mast cell growth factor gene expression in a lineage associated manner , and loss of certain types of genes such as NME in teratomas that may act in a dominant negative fashion in differentiation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
This effect could not be induced in HMC 1 cells by the well known mast cell growth factor stem cell factor ( SCF ) , probably due to mutations of the SCF receptor c Kit in these cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Recently , two activating mutations in the intracellular domain of the proto oncogene c kit , which encodes a tyrosine kinase receptor for the mast cell growth factor stem cell factor , have been detected in the human leukemic mast cell line HMC 1 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell c kit tyrosine kinase ( also referred to as mast cell growth factor , stem cell factor , or steel factor ) in combination with autocrine and paracrine growth factors and cytokines ( IL 3 , IL 4 , IL 5 , IL 6 , IL 7 , G CSF , etc . ) stimulates myelopoiesis , including erythropoiesis , as well as lymphopoiesis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The c KIT protooncogene encodes KIT , a tyrosine kinase that is the receptor for mast cell growth factor . ^^^ All patients with adult sporadic mastocytosis had somatic c KIT mutations in codon 816 causing substitution of valine for aspartate and spontaneous activation of mast cell growth factor receptor ( P = 0 . 0001 ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Phenotypic similarities to some pigmentation disorders in human and mouse , combined with comparative mapping data , identified KIT , encoding the mast cell growth factor receptor , as a major candidate gene for the roan locus ( Rn ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
These include the mast cell enzyme tryptase , CD 2 , and mast cell growth factor receptor c kit ( CD 117 ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In all adults , but not children with indolent mastocytosis , we found activating point mutations of c kit , the mast cell growth factor receptor , in lesional cutaneous mast cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
GISTs commonly have activating mutations in exon 11 ( or rarely exon 9 and exon 13 ) of the KIT gene that encodes a tyrosine kinase receptor for the growth factor named stem cell factor or mast cell growth factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
GISTs commonly have activating mutations in exon 11 ( or rarely exon 9 and exon 13 ) of the KIT gene that encodes a tyrosine kinase receptor for the stem cell factor or mast cell growth factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In mouse embryos , MPs express the receptor tyrosine kinase , KIT , and require its cognate ligand , Mast cell growth factor ( MGF ) , for survival and differentiation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Furthermore , the presence of stem cell factor ( SCF ) , the main mast cell growth factor , and of its receptor , c kit , was also assessed . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Activating mutations of KIT ( formerly c kit ) , the receptor of the mast cell growth factor stem cell factor ( SCF ) , appear to play a key role in the pathogenesis of sporadic adult onset mastocytosis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In fact , the tumor exhibited strong immunoreactivity for the mast cell growth factor receptor ( CD 117 ) in mast cells , basal melanocytes , and nevus nests . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
OBJECTIVE : To elucidate the role of mast cells in the pathogenesis of rheumatoid arthritis ( RA ) and to assess whether apoptosis of cultured and synovial tissue mast cells can be induced by inhibiting mast cell growth factor receptor , c kit tyrosine kinase . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The ligand for KIT , stem cell factor ( SCF ) , also known as steel factor or mast cell growth factor promotes the dimerisation and autophosphorylation of KIT receptors . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The KIT and MGF genes have been shown to play key roles in embryonal and postnatal development of germ cells ; therefore , we evaluated their expression by Northern blot analysis in a panel of three GCT cell lines and 24 fresh GCT biopsies . ^^^ Deregulated expression of MGF and KIT , which was discordant between seminomatous and nonseminomatous lesions , was observed . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In the mouse , genes residing in three loci in which inactivation mutations lead to piebaldism , the dominant spotting ( W ) , patch ( Ph ) , and Sl encode , respectively , the receptor kinases c kit and platelet derived growth factor receptor , and the ligand for c kit : MGF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Analysis of several factor dependent myeloid cell lines indicated that JAK 2 is physically associated with the common beta subunit and with MGF receptor ( c Kit ) even prior to ligand binding . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Of the patients without apparent KIT mutations , none have apparent abnormalities of the gene encoding SLF itself ( MGF ) , and genetic linkage analyses in two of these families are suggestive of linkage of the piebald phenotype to KIT . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The Steel ( Sl ) locus is essential for the development of germ cells , hematopoietic cells , and melanocytes and encodes a growth factor ( Mgf ) that is the ligand for c kit , a receptor tyrosine kinase encoded by the W locus . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Fluorescence activated cell sorting ( FACS ) analysis with anti c kit antibodies revealed MGF receptor expression in eight out of nine cases , often in a subpopulation of the cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Lack of mutations of the MGF and KIT genes in Diamond Blackfan anemia . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Expression of MGF receptor , the c kit protein , was also measured by flow cytometry . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The development of neural crest derived melanocytes , as well as haematopoietic and germ cells , is affected by mutations of the Kit and Mgf genes , which lead to dominant spotting ( W ) or steel ( Sl ) phenotypes . ^^^ Mgf codes for the ligand of the receptor tyrosine kinase encoded by the Kit locus . ^^^ Our transgenic lines have simplified the study of melanoblasts in the mouse embryo , and by crossing our mice with KitW 5 mutants we have been able to identify the midgestation stages at which melanoblasts rely critically on Mgf / Kit interactions . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Here , we describe that the dominant white phenotype in domestic pigs is caused by two mutations in the KIT gene encoding the mast / stem cell growth factor receptor ( MGF ) , one gene duplication associated with a partially dominant phenotype and a splice mutation in one of the copies leading to the fully dominant allele . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The development of clinical signs of anaphylaxis and extent of gastric neutrophil infiltration was similar in mast cell deficient Kit ( W ) / Kit ( W 5 ) or Mgf ( Sl ) / Mgf ( Sl d ) mice and the respective normal congenic mice . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mutations of the receptor tyrosine kinase , Kit , or its ligand , mast growth factor ( Mgf ) , affect three unrelated cell populations : melanocytes , germ cells , and mast cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Crosses of Ednrb ( s ) with Kit ( WJ 2 ) ( the receptor for MGF ) deficient mice confirmed the hypothesis that synergistic interaction between the Endothelin and MGF signaling pathways regulates proper neural crest derived melanocyte development in vivo . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The tyrosine kinase receptor Kit and its ligand Mgf ( Steel Factor ) are essential for melanoblast survival and proliferation during their migration from the neural crest . ^^^ Inappropriate Kit expression in the dermatome and dermis of patch and rump white mouse mutants apparently sequester Mgf , inhibiting melanoblast dispersal . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mutations in KIT encoding the mast / stem cell growth factor receptor ( MGF ) are responsible for coat color variation in domestic pigs . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Analyzing mast cell development and function using mice carrying mutations at W / c kit or Sl / MGF ( SCF ) loci . ^^^ In W / Wv mice , the cells which ordinarily give rise to normal mast cell populations do not adequately respond to a major migration , survival , proliferation and maturation factor expressed in the microenvironments where mast cells ordinarily develop : the c kit receptor ligand , SCF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Improved in vitro erythropoiesis with SCF in all types of inherited marrow failure syndromes does not suggest a common defect involving kit or SCF , but implies that SCF may be helpful in the treatment of hematopoietic defects of varied etiologies . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
These data show that , although c kit is found both on the primitive hematopoietic stem cells and progenitors , Sca 1+ cells are more primitive and respond better than Sca 1 cells to a combination of hematopoietic factors , including SCF and stromal cells . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
As Diamond Blackfan anemia shares clinical features with W and Steel defects in mice , we investigated the possibility that this human disorder might result from an abnormality of the c kit receptor or its ligand , stem cell factor ( SCF ) . ^^^ For these studies , full nucleotide sequences for coding regions of c kit and SCF were generated for two Diamond Blackfan anemia patients and were normal . ^^^ These data provide evidence that Diamond Blackfan anemia does not result from structural abnormalities of c kit or SCF . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The receptor for SCF is encoded by the protooncogene c kit . ^^^ This MoAb , designated SR 1 , blocks binding of 125I human SCF to the c kit receptor , and neutralizes the biologic effects of SCF in hematopoietic colony assays . ^^^ With few exceptions , c kit expression was identified on all hematopoietic and lymphoid cell lines tested by indirect immunofluorescent analysis using SR 1 and by binding studies with 125I SCF . ^^^ This MoAb may be useful to characterize the spectrum of cells that display the c kit receptor and to further define the role of SCF in hematopoiesis . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Hemopoietic stem cell factor ( SCF ) , which is the ligand for the proto oncogene c kit receptor ( allelic with W locus ) and the product of Sl locus of the mouse , has recently been cloned . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Our previous analysis of the signal transduction pathway used by the c kit encoded receptor for the stem cell factor ( SCF ) indicated efficient coupling to the type 1 phosphatidylinositol 3 ' kinase ( PI3K ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
SCF , a known ligand for the c kit tyrosine kinase receptor , was unable to stimulate motility in W 42 mutant mast cells , which have a defective c kit tyrosine kinase . ^^^ These results show that SCF is a chemotactic factor as well as a growth factor and that the c kit receptor can transduce signals leading to both cell proliferation and increased directional cell motility . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The SCF and c kit genes in Diamond Blackfan anemia . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The ligand for the product of the c kit proto oncogene ( also known as stem cell factor or SCF ) , among other activities , has been reported to have similar effects on erythroid colony growth . ^^^ Zsebo , Amgen Inc . ) we now show that cells derived from CFU RF colonies express SCF but not c kit . ^^^ Cells of the human erythroleukemia cell line HEL were found to express c kit but not SCF . ^^^ Neither c kit nor SCF mRNA were detected in phytohemagglutinin stimulated lymphocytes . ^^^ Together , these data support the view that the behaviour of proliferating erythroid stem cells in the marrow , which may express c kit , could be regulated by membrane bound SCF present on surrounding stromal cells . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
SCF receptors ( c kit receptors ) were quantitated by equilibrium binding studies with 125I SCF , and binding parameters were estimated using the ligand program . ^^^ These results identify c kit receptors on human AML blasts , and indicate that SCF acts synergistically with IL 3 or GM CSF to stimulate colony growth from the marrow cells of a portion of patients with AML . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
These effects are performed by activation of the SCF receptor , c kit . ^^^ To determine whether human tumor cell lines display c kit receptors , we performed binding experiments with 125I SCF on a breast carcinoma cell line ( Du 4475 ) , a gastric carcinoma cell line ( KATO 3 ) , a melanoma cell line ( HTT 144 ) , as well as two small cell lung carcinoma cell lines ( H 69 and H 128 ) . ^^^ These data show that some human solid tumor cell lines display high affinity c kit receptors and produce SCF , which can be detected on the cell surface . ^^^ These results suggest the possibility that autocrine production of SCF by c kit receptor bearing tumor cells may enhance cell growth in tumor cell lines . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We have examined the effect of prestimulation of bone marrow with various cytokines , including the product of the Steel gene , Steel factor or stem cell factor ( SCF ) ( the ligand for the c kit receptor ) on the efficiency of retroviral transduction of the human adenosine deaminase ( hADA ) cDNA into murine HSC . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The protooncogene c kit encodes a tyrosine kinase receptor for the stem cell factor ( SCF ) . ^^^ Although receptor dimerization can be attributed to the dimeric nature of the ligand , no dissociation of Kit dimers occurred at high excess of SCF , suggesting that receptor receptor interactions are also involved in dimer stabilization . ^^^ This was supported by in vitro formation of heterodimers between the human and murine Kit proteins through monovalent binding of species specific human SCF . ^^^ By coexpression of human and mouse Kit in murine fibroblasts , we found that receptor heterodimerization in living cells involved an increase in the affinity of human Kit for rat SCF and also an accelerated rate of receptor down regulation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
All of these primitive cells express c kit product and the direct action of SCF is expected . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Because the interaction of c kit product with its ligand ( stem cell factor [ SCF ] ) is known to play an important role not only in proliferation and differentiation of mast cells but also in attachment of CMC to fibroblasts , the expression and function of c kit were investigated in mi / mi CMC and + / + CMC . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We have also provided biological and physical evidence that SCF is a ligand for the c kit receptor . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The haematopoietic growth factor SCF ( stem cell factor ) has been identified as the product of the Sl locus and a ligand for c kit . ^^^ Both SCF and c kit are also expressed in a variety of other tissues , including the brain and spinal cord , suggesting that the receptor ligand system has additional roles in embryogenesis . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , a novel hematopoietic growth factor and ligand for c kit tyrosine kinase receptor , maps on human chromosome 12 between 12q14 . 3 and 12qter . ^^^ Recently a novel hematopoietic growth factor , stem cell factor ( SCF ) , was cloned and demonstrated to be the ligand for the c kit tyrosine kinase receptor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , the ligand for the c kit protooncogene product , was able to stimulate blast cell and granulocytic colony formation by precursors from normal murine bone marrow . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) is the ligand for the receptor encoded by the c kit proto oncogene . ^^^ Mutations of either c kit or the SCF gene are responsible for the defects of W and SI mutant mice , which both suffer a macrocytic anemia , the former associated with defective stem cells and the latter with a defective hematopoietic microenvironment . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The proto oncogene c kit encodes a transmembrane tyrosine kinase receptor for stem cell factor ( SCF ) . ^^^ The c kit / SCF signal is expected to have an important role in hematopoiesis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Recently , a novel cytokine designated stem cell factor ( SCF ) was isolated from medium conditioned by buffalo rat liver cells and proved to be the ligand for c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To provide insights into the pathogenesis of Diamond Blackfan anemia , we examined the in vitro response of erythroid progenitors to the recently isolated ligand for c kit ( stem cell factor , SCF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Recently the ligand for the c kit product , stem cell factor ( SCF ) , was identified in both transmembrane and soluble forms . ^^^ In order to examine the mechanism for receptor activation by SCF and biological properties of the activated c kit product , we transfected the wild type human c kit cDNA into porcine aortic endothelial cells . ^^^ We also demonstrate that SCF induces dimerization of the c kit product in intact cells , and that dimerization of the receptor is correlated with activation of its kinase . ^^^ Activation of the c kit product by SCF was found to induce circular actin reorganization indistinguishable from that mediated by the PDGF beta receptor in response to PDGF BB . ^^^ Furthermore , soluble SCF was a potent chemotactic agent for cells expressing the c kit product , a property which might be of importance during embryonic development . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) is a pluripotent growth factor which is suggested to play an important role in proliferation and differentiation in various types of fetal and adult tissues as the ligand of the c kit proto oncogene product . ^^^ Now we report that the SCF gene is expressed in a wide variety of human cancers including lung cancer , in marked contrast to c kit , which is expressed in very few types of cancers . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem Cell Factor ( SCF ) , a multipotent growth factor which is the ligand for c kit , synergizes with these multiple factor combinations to increase colony number and size . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
SCF is a product of the steel ( Sl ) locus of the mouse , and it is a ligand for the c kit proto oncogene receptor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The Kit tyrosine kinase ( Kit ) encoded by the c kit proto oncogene is a receptor for stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Immunohistochemical localisation of stem cell factor ( SCF ) with comparison of its receptor c Kit proto oncogene product ( c KIT ) in melanocytic tumours . ^^^ In order to characterise the distribution and role of stem cell factor ( SCF ) , a recently reported growth factor for normal melanocytes , we carried out an immunohistochemical study on benign and malignant melanocytic tumours with a comparison with the presence of its receptor c Kit proto oncogene product ( c KIT ) . ^^^ In normal skin , SCF was mainly observed in endothelial cells of blood vessels but not frequently in basal melanocytes , whereas c KIT was predominantly localised in tissue mast cells . ^^^ In benign neoplastic melanocytes ( common melanocytic naevi ) , localisation of SCF and c KIT was complementary : SCF was mostly found in dermal naevus cells while c KIT was revealed in epidermal naevus cells , although the expression of the latter antigen was not frequent . ^^^ Of five cultured melanoma cell lines , SCF was observed in only one , and c KIT was not found in any melanoma cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
By employing a monoclonal antibody against the stem cell factor receptor ( SCF R ) , c kit oncogene product , we analysed in flow cytometric technique the density of SCF R on GM / SO cells which were incubated under various culture conditions . ^^^ Since the most striking difference regarding density of SCF R between GM CSF treated and untreated cells was observed on day 2 , the modulation of c kit oncogene protein by GM CSF likely occur prior to expression of protein onto the cell surface . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We investigated the in vitro effect of stem cell factor ( SCF ) , the ligand for c kit receptor , on DRGs . ^^^ Recombinant murine SCF ( rmSCF ) induced the outgrowth of c kit positive neurites from DRGs of normal ( + / + ) embryos . ^^^ The effect of SCF was dose dependent and completely abolished by anti c kit ACK 2 monoclonal antibody ( mAb ) . ^^^ Most c kit positive neurons appeared to respond to NGF as well , and the SCF responsive subpopulation represented about 10 % of NGF responsive neurons . rmSCF did not support the survival of DRG neurons from embryos of W / W and W42 / W42 genotypes . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The extracellular domain of c kit , which is the receptor for stem cell factor ( SCF ) , was fused genetically to the Fc portion of human immunoglobulin G 1 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
SCF / c kit receptor mediated arachidonic acid liberation in rat mast cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Next , we investigated the effects of stem cell factor ( SCF ) on the differentiation of the K 562 cell expressing c kit . ^^^ On the other hand , SCF suppressed induction of benzidine positive cells when c kit positive cells were treated with hemin and Ara C , especially at a low concentration . ^^^ SCF also downregulated the c kit proteins . ^^^ Our results suggest that the SCF / c kit signals could act negatively for erythroid differentiation of the K 562 cells expressing c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
B8 , we have examined the expression of the c kit protein , the receptor for the hematopoietic cytokine stem cell factor ( SCF ) , on primitive hematopoietic cells . ^^^ Since SCF has been reported to act as a growth factor for early lymphoid cells as well as myeloid cells , we looked for coexpression of c kit and early lymphoid markers in the CD34+ population by multiparameter flow cytometry . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , the ligand of the c kit receptor , is a potent enhancing cytokine for haematopoietic cells in the presence of IL 3 , GM CSF and erythropoietin ( Epo ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The role of stem cell factor ( SCF ) and its receptor ( c kit ) in the intestinal secretory response to cholera toxin ( CT ) was investigated using a ligated intestinal loop model in mice having mutations in the dominant white spotting ( W ) locus and the steel ( Sl ) locus . ^^^ Taken together , the above results suggest that CT induced intestinal secretory responses are dependent upon SCF c kit interactions . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To define the mechanism of receptor activation , chimaeric receptors were constructed from the intracellular region of either IL 2R beta or IL 2R gamma and the extracellular region of c kit , a receptor tyrosine kinase that homodimerizes on binding stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The GM / SO cell line bears a high level of stem cell factor receptors ( SCF R ) i . e . c kit protein , and therefore constitutes a potential model for studying the regulation of this crucial receptor on myeloid cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The hemopoietic cytokines IL 3 ( interleukin 3 ) , IL 5 and GM CSF ( granulocyte macrophage colony stimulating factor ) are involved in the regulation of basophils ( and eosinophils ) , whereas the ligand for c kit , SCF ( stem cell factor ) is a mast cell specific agonist . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
SCF triggers cell growth by binding to the c kit receptor . ^^^ Because endothelial cells can respond to certain hematopoietic growth factors , we tested human umbilical vein endothelial cells for display of the c kit receptor and examined the effect of SCF on endothelial cell proliferation , adhesion molecule expression , and production of tissue factor . ^^^ Quantitative binding experiments with 125I SCF showed both high affinity ( Kd = 42 pmol / L ) and low affinity ( Kd = 1 . 7 nmol / L ) c kit receptors . ^^^ Enzyme immunoassays showed that endothelial cells released soluble c kit receptor and SCF . ^^^ The neutralizing anti c kit receptor monoclonal antibody SR 1 blocked binding of 125I SCF to the c kit receptor by 98 % but did not alter endothelial cell proliferation or adhesion molecule expression . c kit receptors were also detected on adult endothelial cells lining small blood vessels in normal human lymph nodes . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
BACKGROUND : Stem cell factor ( SCF ) and its receptor , c kit , are known to play important roles in hematopoiesis , melanogenesis , and gametogenesis . ^^^ The biologic effects of the SCF / c kit system are believed to involve survival , proliferation , and migration of early stem cell progeny . ^^^ Although SCF and c kit receptor are widely expressed during normal embryonic development , their expression in the adult is limited . ^^^ EXPERIMENTAL DESIGN : The expression of SCF and c kit genes was examined during liver regeneration via the oval cell compartment utilizing partial hepatectomy ( PH ) combined with the administration of a noncarcinogenic dose of 2 acetylaminofluorene ( AAF ) for 8 days ( AAF / PH model ) . ^^^ RESULTS : Both the ligand and the receptor genes were expressed during the early stages of oval cell proliferation after partial hepatectomy in the AAF / PH model , while neither simple partial hepatectomy nor AAF administration alone induced a noticeable expression of the SCF / c kit system . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The interaction between c kit receptor and its corresponding ligand , stem cell factor ( SCF ) , has been suggested to be involved in embryogenesis as well as carcinogenesis via the autocrine / paracrine system . ^^^ In the present study , cancer cell lines and normal / benign / malignant tissues of the human female genital tract were examined for the expression of both c kit and SCF by Northern blot and immunohistochemical analyses . ^^^ Two of 16 cell lines showed mRNA expression of both c kit and SCF , while 2 and 12 cell lines expressed c kit and SCF , respectively . ^^^ In tissues , several cases of malignant tumors , including three cervical cancers , one ovarian cancer , and one ovarian immature teratoma , expressed mRNA of both c kit and SCF . ^^^ In normal tissues , squamous epithelium expressed SCF immunohistochemically , while c kit protein was detected only in melanocytes . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The cell surface receptor c kit and its cognate ligand stem cell factor ( SCF ) or steel factor ( SLF ) are important for the maintenance of hematopoiesis both in vitro and in vivo . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , a ligand for c kit , has a broad range of activities including effects on cells at or near the level of the multipotential stem cell as well as on committed cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The mRNA expression for c kit ( by Northern blot analysis ) and 125I SCF binding on bone marrow cells was elevated within 2 and 4 h of IL 1 administration respectively . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
IL 3 dependent mast cells undergo apoptosis upon removal of IL 3 , an event that is prevented by the addition of stem cell factor ( SCF ) acting through its receptor c kit , suggesting that SCF provides a mechanism to allow mast cells to survive and to differentiate in tissues in the relative absence of IL 3 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The downregulation of Kit , the surface receptor for SCF , is probably a critical factor , because cells lacking this molecule would not be able to respond to SCF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) is hypothesized to play a critical role in the migration of melanocytes during embryogenesis because mutations in either the SCF gene , or its ligand , c kit , result in defects in coat pigmentation in mice and in skin pigmentation in humans . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Molecular genetic alterations of known protoncogenes and growth factors , e . g . c kit and its ligand SCF as well as hst 1 and c myc , are likely to play a role in the development of testicular cancer . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Here , we studied the interactions between TNF alpha and the stem cell factor ( SCF ) receptor , c kit , in normal CD34+ hematopoietic progenitors and their leukemic counterpart , ie , acute myeloid leukemic ( AML ) CD34+ cells coexpressing c kit antigen . ^^^ The results showed that ( 1 ) incubation of normal bone marrow mononuclear cells with 200 U / mL rhTNF alpha for 20 hours induced a diminution of 31 . 2 % + / 5 . 2 % of CD34+ cells coexpressing c kit ; ( 2 ) the same decrease was observed using purified CD34+ cells and , furthermore , their proliferative response to SCF was inhibited by 31 . 5 % + / 7 . 3 % after exposure to TNF alpha ; ( 3 ) similar experiments performed on CD34+ c kit+ AML cells from 11 patients gave comparable results . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
During development , mice with mutations of stem cell factor ( SCF ) or its receptor c kit exhibit defects in melanogenesis , as well as hematopoiesis and gonadogenesis . ^^^ Because melanocytes derive from neural crest cells , the role of SCF and c kit was investigated in the neural crest derived childhood tumor neuroblastoma . ^^^ Using reverse transcription polymerase chain reaction analysis , simultaneous expression of steady state mRNA for the SCF ligand and its receptor c kit was found in 14 of 14 ( 100 % ) human neuroblastoma cell lines and clones and in 8 of 18 ( 45 % ) human neuroblastoma tumor samples . ^^^ Simultaneous expression of SCF and c kit mRNA in both neuroblastoma cell lines and tumors implies that c kit may act as part of an autocrine growth loop in conjunction with endogenous production of SCF in this disease . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The cells have the morphology of immature mast cells , are Toluidine blue positive , are karyotypically normal , are CD33+ , CD 34 , CD45+ , c kit , and c fms , and die in the absence of either SCF or IL 3 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Cross linking of either IgE receptor or SCF receptor ( c kit ) on CMC resulted in histamine secretion ( non specific release : < 6 % of total histamine , alpha IgE induced : 12 % to 52 % ; SCF induced release : 9 % to 18 % ) , whereas neither substance P ( a skin MC agonist ) nor the basophil agonist FMLP showed an effect on CMC . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) plays a crucial role in hematopoiesis through its interaction with the receptor tyrosine kinase c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The SCF receptor protein has been identified as the product of the protooncogene c kit and has been detected using monoclonal antibodies ( MAbs ) on a broad selection of erythroid , myeloid , and lymphoid cell lines as well as on bone marrow mononuclear cells ( BMMNC ) . ^^^ A detailed study of the involvement of SCF and its receptor c kit in normal erythropoiesis will help elucidate intrinsic irregularities of anemias such as Diamond Blackfan Anemia , an aregenerative congenital anemia . ^^^ This report describes a nonradioactive method for detecting SCF receptors that varies from conventional assays in that the fluorescent label conjugated to the SCF / c kit complex is connected via an extended ester linkage that reduces steric influence and promotes full normal structural ligand binding of the SCF to its receptor . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Soluble kit receptor in human serum . c kit encodes the transmembrane receptor tyrosine kinase ( Kit ) for the recently described ligand stem cell factor ( SCF ) . ^^^ The immunoaffinity purified serum derived soluble Kit inhibited binding of [ 125I ] SCF to membrane bound receptor in an in vitro assay . ^^^ These results indicate that soluble Kit could modulate the activity and functions of SCF in vivo . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , the ligand for the c kit proto oncogene , has been shown to play a critical role in the migration of melanocytes and germ cells during embryogenesis as well as in the proliferative control of the hematopoietic compartment . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Localization of stem cell factor ( SCF ) and c kit mRNA in human placental tissue and biological effects of SCF on DNA synthesis in primary cultured cytotrophoblasts . ^^^ When the distribution of SCF mRNA and c kit mRNA in the placental tissues was examined by in situ hybridization , SCF mRNA was detected in the cytotrophoblast , the intermediate trophoblastic cell column and the stromal cells , while c kit mRNA was detected in the cytotrophoblast and the intermediate trophoblastic cell column . ^^^ Both c kit and SCF mRNA were absent or very weak in the syncytiotrophoblasts . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
On the other hand , SCF did not stimulate colony growth in any of the four cases which were not positive for the c kit molecule . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Expression of the c kit proto oncogene and its ligand stem cell factor ( SCF ) in normal and malignant human testicular tissue . ^^^ Recent findings suggest an important role of the proto oncogene c kit , a surface membrane receptor of the tyrosine kinase family , and its ligand stem cell factor ( SCF ) in normal spermatogenesis and possibly in the pathogenesis of certain testicular germ cell tumors . ^^^ To further investigate this potential role , the expression of c kit and SCF was studied in normal and malignant human testicular tissue specimens at the mRNA and protein level by Northern blot analysis and immunohistochemistry , respectively . ^^^ The detection of the c kit receptor in normal human germ cells and its natural ligand SCF in Sertoli cells suggests the presence of a local trophic regulatory system that may be active in human spermatogenesis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Cultured mast cells ( CMC ) derived from the bone marrow of mice express the receptor encoded by the W ( c kit ) locus ( W receptor ) , and the WCB6F1 ( + / + ) 3T3 fibroblasts express the ligand encoded by the Sl locus ( stem cell factor [ SCF ] ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
KIT+ lineage cells cultured with SCF and IL 3 generate numerous mast cells , whereas equivalent numbers of KIT cells or naive bone marrow cells do not . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Marrow stromal layers , which constitute part of these culture systems , produce growth factors , including stem cell factor ( SCF ) , a ligand for the c kit proto oncogene that has been found to increase detection of myeloid , erythroid , and megakaryocytic progenitors in short term marrow colony assays . ^^^ This increase in CFU GM could be inhibited specifically with the addition of 1 : 1500 SR 1 , a neutralizing anti c kit monoclonal antibody ( MAb ) that neutralizes the biological effects of SCF , and the increase was noted both with recharged light density marrow cells and purified CD34+ progenitor cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The gene products of these loci are a peptide growth factor , called here stem cell factor ( SCF ) , and its tyrosine kinase receptor , the proto oncogene c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) is a ligand for c kit receptor and has a critical role in the development of mast cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The presence of receptors for SCF in ALL was confirmed by polymerase chain reaction for c kit mRNA in 19 / 21 cases tested . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Downregulation of c kit is invariably associated with successful mobilization which , when combined with data from in vitro studies , implies a key role for stem cell factor ( SCF ) as an orchestrator of mobilization . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In the cells , KITG 559 or KITV 814 were found to be constitutively phophorylated on tyrosine in the absence of stem cell factor ( SCF ) that is a ligand for KIT . ^^^ Chemical cross linking analysis showed that a substantial fraction of the phosphorylated KITG 559 underwent dimerization even in the absence of SCF , whereas the phosphorylated KITV 814 did not , suggesting the distinct mechanisms underlying constitutive activation of KIT by G 559 and V 814 mutations . ^^^ Furthermore , the cells expressing either KITG 559 or KITV 814 were found to show a factor independent growth , whereas the cells expressing wild type KIT ( KITWT ) proliferated in response to SCF as well as IL 3 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In this study , we showed that SCF also supported the growth of UT 7 in the absence of other cytokines and downregulated the cell surface c kit receptors . ^^^ These results suggest that SCF stimulated UT 7 proliferation partially through the autocrine loop of GM CSF , and UT 7scf9 expressed SCF mostly as a membrane bound form , which transduces its growth signal through c kit receptor as they aggregate by cell to cell interaction . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Numerous studies have demonstrated the ability of stem cell factor ( SCF ) , a key regulator of hematopoiesis signaling through c kit , to synergize with other hematopoietic growth factors , but little is known about cytokines capable of inhibiting hematopoiesis induced by SCF . ^^^ SCF stimulated proliferation of the hematopoietic cell line FDC P 1 was also potently inhibited by TNF alpha and was accompanied by down regulation of c kit cell surface expression as well as c kit mRNA levels . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) triggers cell growth by binding to cell surface c kit receptors . ^^^ Quantitative binding experiments with 125I SCF indicate that the soluble c kit receptors obtained from human serum or OCIM 1 cells have binding affinities for SCF of approximately 200 to 300 pmol / L , in contrast to the recombinant form , which has a binding affinity of approximately 1 . 5 nmol / L . ^^^ All three forms of the soluble c kit receptor were able to compete with c kit receptors on OCIM 1 cells for 125I SCF binding . ^^^ Thus human hematopoietic cells can produce a soluble form of the c kit receptor that retains high affinity SCF binding activity . ^^^ We speculate that the soluble c kit receptor may bind SCF and function as a receptor antagonist in vivo . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
When rat wild type c kit cDNA and mutant type c kit cDNA encoding KITTyr 817 were transfected into cells of a human embryonic kidney cell line ( 293T ) , only mutant form KITTyr 817 was constitutively phosphorylated on tyrosine and activated in the absence of SCF . ^^^ In RBL 2H3 cells , KIT was constitutively phosphorylated on tyrosine and activated in the absence of autocrine production of its ligand , stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , a hematopoietic growth factor , is the ligand of the tyrosine kinase receptor encoded by the c kit proto oncogene . ^^^ We have studied the expression of SCF and c kit in 20 human malignant glioma cell lines at the mRNA as well as at the protein level . ^^^ SCF and c Kit proteins were detected in the cytoplasm of glioma cells by alkaline phosphatase monoclonal anti alkaline phosphatase immunostaining and Western blot analysis . ^^^ However , neither SCF nor c Kit were seen on the cell surface by flow cytometry . ^^^ These results suggest that SCF and c Kit may mediate non proliferative signals or may employ intracellular mechanisms for autocrine growth regulation of glioma cells . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
These studies further suggest that interaction of the c kit receptor and its ligand ( stem cell factor , SCF ) is critical for the development of the enteric nervous system . ^^^ The authors investigated the presence of c kit positive ( c kit+ ) cells as well as the expression of SCF in bowel from 12 patients with Hirschsprung ' s disease ( HD ) , 4 patients with total colonic aganglionosis ( TCA ) , 2 patients with extensive aganglionosis ( EA ) and 14 controls . ^^^ Our methods involved the use of immunohistochemistry with antihuman c kit sera and antihuman SCF sera . ^^^ A lack of c kit and SCF might be of significance for autonomic gut dysmotility in aganglionic bowel segments of patients with HD and allied disorders such as chronic idiopathic intestinal pseudo obstruction . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
MEG 01s cell proliferation or survival was only marginally influenced by these factors and their combinations . c kit , the receptor for SCF , was downmodulated by its ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , the ligand for the c kit tyrosine kinase receptor , markedly stimulates the accumulation of erythroid progenitor cells in vitro . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The apoptotic changes following IL 3 deprivation are prevented by the addition of SCF which exerts its rescue effect upon interaction with its c Kit tyrosine kinase receptor . ^^^ Transforming growth factor beta was found to specifically prevent this SCF mediated rescue from apoptosis , probably by down regulating the expression of c Kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , the ligand for the receptor ( SCFR ) that is encoded by the c kit proto oncogene , has many important effects in mouse and human mast cell development , survival , and function . ^^^ SCF can promote mast cell survival by suppressing apoptosis , induce mast cell hyperplasia in murine rodents , experimental primates and humans , directly induce SCFR dependent mast cell mediator release , and significantly modulate the extent of mast cell activation by Fc epsilon RI dependent mechanisms . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Recombinant human stem cell factor ( SCF ) binds to the c kit receptor on human bone marrow progenitor cells and enhances their survival following irradiation . ^^^ Since the c kit receptor has also been detected on malignant cells , experiments were performed to study the effect of SCF on the proliferation and radiation survival of a variety of both c kit positive and negative human tumor cell lines using [ 3H ] thymidine incorporation and colony formation assays . ^^^ The addition of SCF to both c kit positive and negative cell line cultures had no significant effect on the stimulation index ( in [ 3H ] thymidine assay ) . ^^^ Furthermore , SCF did not significantly increase the surviving fraction of either c kit positive or negative cell lines following radiation , and there were no statistically significant differences between D 0 [ defined by the slope of the terminal exponential region of the two component ( single hit multitarget model ) survival curve where slope = 1 / D0 ] , Dq ( quasithreshold dose ) , n ( extrapolation number ) , alpha , and beta values for any of the cell lines studied that were irradiated with and without SCF . ^^^ Finally , nude mice with transplanted human LG 425 cutaneous T cell lymphoma ( c kit positive ) were treated with 10 Gy with or without SCF ( 100 micrograms / kg i . p . 20 h before , 2 h before , and 4 h after irradiation ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Previously , we showed that c kit receptor tyrosine kinase is expressed by a subpopulation of dorsal root ganglion ( DRG ) neurons , and that the ligand for the c kit receptor , stem cell factor ( SCF ) , induces the neurite outgrowth and supports the survival of these neurons in culture [ 16 ] . ^^^ When DRG cells of embryonic day 15 . 5 or 17 . 5 were cultured , the survival of c kit positive neurons was supported by SCF , nerve growth factor ( NGF ) or leukemia inhibitory factor . ^^^ SCF and NGF synergistically supported the survival of c kit positive neurons at submaximal concentrations . c kit positive DRG neurons from neonatal mice survived without addition of any factor in culture , suggesting that the requirement for trophic support in c kit positive neurons changes during development . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Immunohistochemical stains revealed an increase in staining with melanocyte specific monoclonal antibodies HMB 45 and NKI / beteb , and a monoclonal antibody to the receptor for SCF , c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Changes in c Kit expression and effects of SCF during differentiation of human erythroid progenitor cells . ^^^ In addition , the effects of stem cell factor ( SCF ) on cell surface c Kit expression were analysed in these two culture systems and the effects of SCF on erythroid colony formation were studied in a methylcellulose culture . c Kit was expressed on the cell surface from BFU E to erythroid precursors recognized morphologically as basophilic erythroblasts . ^^^ These results indicate that the c Kit / SCF system still plays in proliferation of erythroid progenitor cells at the colony forming units erythroid stage . ^^^ Finally , expression of c Kit in erythroid progenitor cells cultured without SCF showed a diffuse pattern on the cell surface , whereas we observed positive c Kit immunoreactivity in the region of the Golgi apparatus of these cells cultured with SCF . ^^^ Flow cytometric analysis also showed that the levels of cell surface c Kit expression decreased in the presence of SCF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The FLT 3 gene encodes a receptor tyrosine kinase that is closely related to two well known receptors , KIT and FMS , that regulate with their respective ligands , stem cell factor ( SCF ) and macrophage colony stimulating factor ( M CSF ) , proliferation and differentiation of hematopoietic cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) is the ligand for the tyrosine kinase receptor c kit , which is expressed on both primitive and mature hematopoietic progenitor cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To examine the role of SCF and its receptor , c kit , in the pathogenesis of pediatric Burkitt ' s lymphomas ( BL ) , we investigated the expression of SCF and c kit in BL cells and the mitogenic activity of SCF on BL cells . ^^^ BL cells were stimulated either by Epstein Barr virus ( EBV ) infection or by different reagents and cytokines , and expression of SCF and c kit was studied on the mRNA level by Northern blot analysis and reverse transcriptase polymerase chain reaction ( RT PCR ) , followed by Southern blotting . c kit expression was also studied by fluorescence activated cell sorting and by crosslinking of digoxigenin labeled recombinant human SCF to the cell surface . ^^^ Neither c kit nor SCF could be detected in any other cell line under any condition of stimulation as analyzed by Northern blot analysis , RT PCR followed by Southern blot analysis , crosslinking , and immunofluorescence . ^^^ We conclude that most BL cells express neither SCF nor c kit and that the low level expression of c kit in some BL cells most likely has no biologic significance . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To characterize the production of stem cell factor ( SCF , the ligand for the c kit receptor protein ) and its regulation by inflammatory cytokines and glucocorticoids , primary marrow stromal fibroblasts were isolated from normal individuals and two patients with Diamond Blackfan anemia . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mast cell development in mice is critically regulated by stem cell factor ( SCF ) , the term used here to designate a product of fibroblasts and other cell types that is a ligand for the tyrosine kinase receptor protein encoded by the proto oncogene c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We examined a panel of 25 small cell lung cancer ( SCLC ) cell lines and nude mouse xenografts for expression of the proto oncogenes c met and c kit , and for expression of the corresponding ligands , hepatocyte growth factor ( HGF ) ( also known as scatter factor ( SF ) ) , and stem cell factor ( SCF ) , respectively . ^^^ SCF mRNA was expressed in 19 of the examined tumours , and in 18 of these coexpression of c kit and SCF was present . ^^^ The rare coexpression of c met and HGF / SF is evidence that an autocrine regulatory pathway is not present for this receptor / ligand system in SCLC , while the frequent coexpression of c kit and SCF indicates that this receptor / ligand system may have an autocrine function in SCLC . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The binding of the stem cell factor ( SCF ) to the c kit encoded receptor tyrosine kinase stimulates a variety of biochemical responses that culminate in cellular proliferation , migration , or survival . ^^^ To confine the ligand binding site to this portion of the receptor we generated a panel of murine monoclonal antibodies ( mAbs ) to the Kit protein and identified two mAbs that efficiently displaced receptor bound SCF and also inhibited proliferation of SCF dependent human megakaryocytes . ^^^ Consistent with this conclusion , all of the soluble proteins inhibited SCF binding to Kit expressing cells , and they also underwent specific covalent cross linking to the radiolabeled ligand . ^^^ However , whereas Kit 1 2 3 and Kit 10 displayed comparable ligand affinities , deletion of the third Ig like domain , in Kit 1 2 , involved significant reduction in SCF binding . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Accumulating evidence suggests that c kit and its ligand , stem cell factor ( SCF ) , play an important role in the regulation of at least three lineages of stem cell growth and possibly in leukemogenesis , while only limited data are available that suggest possible involvement of c kit / SCF in the development of human solid tumors such as lung cancer . ^^^ The present study revealed that c kit protein ectopically expressed in SCLC is indistinguishable from that in leukemia cell lines with megakaryocytic characteristics with respect to amount , molecular size , and autophosphorylation status in response to recombinant human SCF . ^^^ Furthermore , significant chemotactic response as well as moderate in vitro cell growth was induced in SCLC cell lines by the addition of recombinant human SCF , suggesting that c kit / SCF may play an important biological role in the development of SCLC . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) is a polypeptide growth factor and the ligand for the c kit proto oncogene product . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The extracellular portion of the kit encoded receptor for the stem cell factor ( SCF ) comprises five immunoglobulin ( Ig ) like domains . ^^^ To localize the ligand recognition site , we exploited the lack of binding of human SCF to the murine receptor by using human mouse hybrids of Kit and species specific monoclonal antibodies ( MAbs ) that inhibit ligand binding . ^^^ By constructing five chimeric murine Kit proteins which individually contain each of these three human Ig like units or pairs of them , we found that the second human domain confers upon the mouse Kit high affinity binding of the human ligand and also binding of species specific SCF competitive MAbs . ^^^ In conclusion , interdomain packing involving the second Ig like domain of human Kit and noncontiguous structural motifs of the receptor are involved in SCF recognition . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) is encoded at the Sl locus of the mouse and is the ligand for the c kit receptor . ^^^ Recombinant rat SCF 164 ( rrSCF 164 ) induces proliferation and promotes maturation of mouse mast cells in vitro and in vivo and can also induce c kit receptor dependent mouse mast cell degranulation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The results indicate that , at least in most of the leukemic myeloid blasts cells , the expression of SCF and its receptor , the c kit oncogene , may permit an autocrine regulation of cell cycling . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
No one could have predicted then that an effort to understand the basis for these abnormalities would ultimately contribute to the characterization of a receptor ( c kit ) and a corresponding ligand ( stem cell factor , SCF ) that are critical not only to the migration and development of melanocytes , but also to hematopoiesis , gametogenesis , mast cell development , and , perhaps , development of the central nervous system . ^^^ Investigation of the effects of SCF on a single cell type , the mast cell , has produced the most complete picture of the spectrum of biological processes that can be regulated by interactions between c kit and its ligand . ^^^ It now seems virtually certain that further studies of c kit and SCF will produce important new insights into problems as diverse as the regulation of lineage commitment during normal hematopoiesis or the development and function of the central nervous system . ^^^ And even though an effect on mast cell development was one of the last phenotypic abnormalities to be recognized in mice with mutations affecting the genes encoding c kit or SCF , mast cells will continue to represent an important model system for analyzing the biology of c kit and its ligand . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The monoclonal rat anti c kit antibody ( ACK 2 ) , which abrogates colony growth supported by stem cell factor ( SCF ) , significantly inhibited the interleukin 6 ( IL 6 ) dependent growth of hematopoietic progenitors derived from spleen cells of normal and 5 fluorouracil ( 5 FU ) treated mice and from bone marrow cells of normal mice in serum containing culture . ^^^ These findings suggest that c kit plays an important role in the growth of hematopoietic progenitors responding to IL 6 , and that SCF in the serum affects the development of hematopoietic progenitors in serum containing cultures . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Because of the prominent role of CSF in the maintenance of normal erythropoiesis in vivo , we examined the effects of SCF on the Epo inducible human erythroleukemia cell line MB 02 , and characterized the c kit receptor in these cells . ^^^ A neutralizing anti c kit receptor monoclonal antibody ( SR 1 ) blocked binding of 125I SCF to MB 02 cells by 98 % , and the effect of SCF on MB 02 growth , c kit receptor binding parameters were quantitated by equilibrium binding experiments with 125I SCF . ^^^ The molecular weight of the c kit receptor was determined by affinity cross linking 125I SCF to MB 02 cells . 125I SCF c kit receptor complexes of approximately 155 , 000 and approximately 310 , 000 daltons were found , likely representing the monomeric and dimeric forms of the c kit receptor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The expression of c kit and its ligand , the stem cell factor ( SCF ) , was studied in five cases of acute myeloid leukemia . ^^^ In this case , the c kit oncogene was overexpressed , but matched by a low expression of its ligand , SCF . ^^^ In one of the other leukemic cell populations studied , characterized by a low expression of c kit and an elevated expression of the SCF , the growth fraction was also very low . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Studies on the mechanism of TGF beta 1 inhibition of SCF induced proliferation of AML blasts revealed that TGF beta 1 treatment of these cells was associated with down regulation of SCF receptor surface expression , as detected with a specific monoclonal antibody , which appeared to be preferentially due to an acceleration of decay of mRNA for the c kit proto oncogene encoding the SCF receptor , without an effect on the overall transcriptional activity of the c kit gene . ^^^ Direct evidence to prove the importance of c kit down regulation in the inhibitory effect of TGF beta 1 on AML growth came also from experiments demonstrating that signal transduction of SCF could be significantly diminished in the presence of TGF beta 1 , as demonstrated by measuring c kit kinase associated phosphorylation of target proteins . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Other molecular genetic alterations have been described , affecting genes that are involved in the normal regulation of spermiogenesis , such as the c kit gene product and its ligand SCF , as well as hst 1 , which is normally expressed in embryonal tissues only . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We have investigated the expression of SCF and its receptor , the c kit proto oncogene , in human colorectal carcinoma cell lines . ^^^ Using reverse transcription PCR , we confirmed the expression of c kit in two lines ( LS174T and LS 1034 ) and of SCF in 9 of 11 cell lines tested . ^^^ Interestingly , IL 4 induced an almost complete down regulation of both c kit and SCF expression in LS174T . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Coordinated mRNA expression of c Kit with tyrosinase and TRP 1 in melanin pigmentation of normal and malignant human melanocytes and transient activation of tyrosinase by Kit / SCF R . ^^^ By incubating these cells with 20 ng / ml of stem cell factor ( SCF ) which is a ligand of c Kit receptor , we found a transient increase of tyrosinase activity 2 4 h post incubation , indicating an early response of tyrosinase activation , either by elevating tyrosinase protein expression or by tyrosinase protein modification ( e . g . phosphorylation ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Since the stem cell factor ( SCF ) induces development of mast cells and since the receptor of SCF is encoded by the c kit gene , we examined the expression of SCF mRNA and c kit mRNA in neurofibroma tissues . ^^^ In situ hybridization demonstrated strong expression of c kit messenger RNA in mast cells in the neurofibroma , but the expression of SCF mRNA was not demonstrable by in situ hybridization in either neurofibroma tissues or control normal skin tissues . ^^^ The results suggest that SCF and the c kit receptor are associated with the increase of mast cells in neurofibroma tissues . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The product of the c kit proto oncogene , denoted Kit / SCF R , encodes a tyrosine kinase receptor for stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
These results emphasize the pivotal role of the KIT and SCF interaction in hematopoiesis and indicate that KITlow cells are more primitive than KIThigh cells . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The interaction of the proto oncogene c kit product with its ligand ( stem cell factor or SCF ) is considered to play crucial roles in hematopoiesis . ^^^ In a series of human acute myeloblastic leukemia ( AML ) cells , the effects of recombinant human ( rh ) SCF on AML cells were examined in short term and long term cultures . c kit expression was detected in 26 of 31 AML cases , and short term treatment of AML cells with rhSCF led to proliferation in 13 of 18 AML cases that expressed the c kit product . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
At least 70 % of small cell lung cancer ( SCLC ) tumors and tumor derived cell lines coexpress the genes for stem cell factor ( SCF ) and its receptor , the c kit proto oncogene . ^^^ To assess the impact of coexpression of the growth factor and receptor on SCLC growth , the NCI H 146 SCLC cell line , which expresses only SCF , was transfected with a c kit expression vector . ^^^ Growth of the c kit transfected cells could be further enhanced by the addition of bombesin or insulin like growth factor 1 , suggesting that the SCF / c kit autocrine loop could function cooperatively with other SCLC autocrine loops . ^^^ To further investigate the importance of this autocrine loop , a cell line that naturally coexpresses SCF and c kit was transfected with a kinase defective c kit gene . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
A common progenitor , termed stem cell factor ( SCF ) progenitor , expresses the receptor for avian SCF c Kit , and undergoes short term self renewal when grown in the presence of avian SCF . ^^^ A second progenitor , referred to as SCF / transforming growth factor alpha progenitor , coexpresses c Kit and the avian epidermal growth factor receptor homologue c ErbB . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Transcripts encoding the receptor for c fms , the receptor for colony stimulating factor 1 ( CSF 1 ) , and c kit ( the receptor for stem cell factor [ SCF ] ) were expressed throughout preimplantation development . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Moreover , the FMA 3 type KIT was dimerized without the stimulation by stem cell factor ( SCF ) , a ligand for KIT . ^^^ The spontaneously dimerized FMA 3 type KIT without SCF binding was not internalized even after the activation . ^^^ IC 2 cells expressing the FMA 3 type KIT grew in suspension culture without IL 3 and SCF and became leukemic in nude athymic mice . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
This phenotype appears to be due to occupancy of murine c kit by human SCF and diminished cell surface expression of endogenous murine SCF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The proto oncogene c kit and its ligand stem cell factor ( SCF ) may play an important role in the development of normal and malignant testicular tissue . ^^^ This study investigates the presence of SCF and c kit protein in 32 orchiectomy specimens of patients with testicular cancer , in 5 specimens of normal testicular tissue and in three established non seminomatous germ cell cancer cell lines ( H12 . 1 , H 32 , 577ML ) by an immunohistochemical approach . ^^^ Out of 9 testicular cancer specimens classified as pure seminomas , 7 ( 78 % ) showed a strong immunohistochemical reaction for both SCF and c kit protein on the surface of the tumour cells . ^^^ Fourteen non seminomatous germ cell tumours composed of embryonal carcinoma were completely negative for both SCF and c kit proteins and only faint positivity was found in 6 tumours ( 26 % ) . ^^^ Differentiated teratomatous structures within the specimens on non seminomatous tumours showed a strong immunohistochemical reaction for SCF and c kit protein in 8 of 11 ( 73 % ) cases . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Kit , the receptor for stem cell factor ( SCF ) and the product of the c kit proto oncogene , is expressed on fetal liver derived mast cell progenitors when cultured with SCF . ^^^ Kit , the receptor for stem cell factor ( SCF ) and the product of the c kit proto oncogene , is expressed on fetal liver derived mast cell progenitors when cultured with SCF . ^^^ Decreased levels of Kit on the surface of human fetal liver derived mast cells after exposure to recombinant human SCF were demonstrated by flow cytometry using the YB5 . ^^^ Internalization of Kit along with SCF appears to be the principal means by which Kit is lost from the mast cell surface . ^^^ Neither the beta 3 integrin CD51 / CD61 ( alpha 5 beta 3 ) , nor the beta 1 integrins CD49d , e / CD29 ( VLA 4 and 5 ) appeared to be internalized along with Kit SCF complexes . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The FLT3R belongs to a family of receptor tyrosine kinases involved in hematopoiesis that also includes KIT , the receptor for SCF ( stem cell factor ) , and FMS . the receptor for M CSF ( macrophage colony stimulating factor ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Whereas coat color changes in the mouse mutants W ( c kit defect ) and Steel ( SCF defect ) and several in vivo and in vitro studies by other investigators have shown previously that SCF is melanotrophic , they also indicated the requirement of an additional factor , or factors , in melanogenesis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In addition to constitutive activation , furthermore , both highly activated substitution mutants ( KITVal 814 and KITTyr 814 ) and modestly activated substitution mutants ( KITGly 814 and KITHis 814 ) were continuously degraded in the absence of SCF , whereas wild type KIT ( KITWild ) required SCF stimulation to undergo degradation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) is a cytokine that is active toward hematopoietic progenitor cells and other cell types , including germ cells , melanocytes , and mast cells , which express its receptor , the tyrosine kinase , Kit . ^^^ SCF exists as noncovalently associated dimer at concentrations where it has been possible to study its quaternary structure ; it stimulates dimerization and autophosphorylation of Kit at the cell surface . ^^^ We have used recombinant versions of human SCF and human Kit extracellular domain ( sKit ) to study SCF Kit interactions . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Together with the previous reports that human sera contain detectable levels of sIL 6R , IL 6 , and SCF , current data suggest that gp 130 signaling in association with c kit activation may play a role in human erythropoiesis in vivo . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We further analysed the cell lines for expression of the proto oncogene C KIT and its ligand SCF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) is a hematopoietic growth factor that interacts with the receptor tyrosine kinase , c kit . ^^^ JAK 2 and c kit were associated in unstimulated cells with further recruitment of JAK 2 to the c kit receptor complex after SCF stimulation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
A role for stem cell factor ( SCF ) : c kit interaction ( s ) in the intestinal tract response to Salmonella typhimurium infection . ^^^ Further , SCF interaction ( s ) with its receptor ( c kit ) was shown to be important for the intestinal tract secretory response after CT exposure . ^^^ Inhibition of Salmonella invasion of epithelial cells was shown to be one potentially important role for SCF : c kit interactions in host defense to Salmonella infection . ^^^ Finally , mice having mutations in the white spotting ( W ) locus , which encodes the SCF receptor ( c kit ) , were significantly more susceptible to oral Salmonella challenge than their control littermates . ^^^ Taken together , the above results suggest that an important intestinal tract response to Salmonella infection is an enhanced production of SCF and its subsequent interactions with c kit . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Although much is now known about the biological properties of the c kit receptor and its ligand , stem cell factor ( SCF ) , little is known of the structural basis for the binding and function of this hematopoietic cytokine . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
As stem cell factor ( SCF ) and its receptor , c kit , are involved in hair pigmentation , SCF is probably produced in the skin , possibly by the regulatory follicular dermal papilla . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) interacts with the receptor tyrosine kinase c Kit and has potent effects on hematopoiesis . ^^^ JAK 2 and c Kit were constitutively associated , and treatment with SCF resulted in rapid and transient tyrosine phosphorylation of JAK 2 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The mRNA expression of the SCF receptor c kit has been shown to be significantly increased in all fresh AML blast cells compared with normal controls ( healthy volunteers ) , in particular CD34+ cells . ^^^ Antibodies blocking c kit reversed the significant inhibitory effect of SCF on chemotherapy induced apoptosis , confirming the role of SCF in the resistance to chemotherapy induced apoptosis in CD34+ human leukemia . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , also known as Steel factor , is a transmembrane cytokine involved in several developmental processes and the ligand for the receptor tyrosine kinase c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) and its receptor Kit encoded by the c kit proto oncogene are crucial for the development and migration of primordial germ cells in rodents . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
A mouse spleen stromal cell line , MSS 62 , can create an in vitro erythropoietic microenvironment in which development of erythropoietin responsive progentor cells is stimulated by cell cell contact via stem cell factor ( SCF ) / c Kit and vascular cell adhesion molecule 1 ( VCAM 1 ) / very late activation antigen 4 ( VLA 4 ) interactions between stromal and erythroid cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Cultured mast cells of mi / mi genotype ( mi / mi CMCs ) did not normally respond to stem cell factor ( SCF ) , a ligand for the c kit receptor tyrosine kinase . ^^^ The poor response of mi / mi CMCs to SCF was attributed to the deficient expression of c kit both the mRNA and protein levels . ^^^ Overexpression of ( + ) MITF but not mi MITF normalized the expression of the c kit and the poor response of mi / mi CMCs to SCF , indicating the involvement of ( + ) MITF in the c kit gene transactivation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The ligand for c kit ( stem cell factor [ SCF ] ) plays a key role in stimulating myeloid and erythroid cell production of primitive hematopoietic progenitors . ^^^ SCF in combination with interleukin 7 ( IL 7 ) can also stimulate the combined myeloid and B cell differentiation of uncommitted hematopoietic progenitor cells as well as the growth of early B cell progenitor cells , although the involvement of c kit in early B lymphopoiesis remains controversial . ^^^ Interestingly , although all FL + IL 7 responsive Lin Sca 1+ progenitor cells and the resulting pro B cells expressed c kit , FL + IL 7 was much more potent ( 62 fold ) than SCF + IL 7 in stimulating production of cells of the B cell lineage . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The use of recombinant SCF protein for rapid determination of c kit expression in normal and abnormal erythropoiesis . ^^^ Stem cell factor ( SCF ) is the ligand for the dimeric c kit tyrosine kinase receptor . ^^^ Binding of SCF to c kit is a crucial element in the developmental stimulus of late stem cells and early progenitor cells . ^^^ In an earlier report we described a rapid non radioactive technique using the extended ester attached labelled SCF protein itself for detecting c kit expression in marrow and peripheral blood mononuclear populations . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The purified CD34+ cells were cultured for 14 days with various combinations of cytokines , including recombinant human macrophage colony stimulating factor ( rM CSF ) , granulocyte CSF ( rG CSF ) , granulocyte macrophage CSF ( rGM CSF ) , interleukin 3 ( rIL 3 ) , and stem cell factor ( SCF ; a ligand for c kit ) in serum free medium . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The phenotypes of mice that harbor a defect in the genes encoding either stem cell factor ( SCF ) or its receptor , c kit , indicate that this ligand / receptor pair is necessary for maintenance of normal hematopoiesis in the adult . ^^^ Monoclonal antibody ACK 2 , which recognizes the murine c kit receptor , was used to selectively block the hematopoietic growth promoting effects of SCF . ^^^ These results suggest that c kit receptor function may be required for optimal response to acute erythropoietic demand and that erythropoiesis in the splenic microenvironment is more dependent on SCF / c kit receptor interaction than is erythropoiesis in the marrow microenvironment . ^^^ These data suggest that interaction of SCF with the c kit receptor affects the homing behavior of hematopoietic progenitor cells in the adult animal . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Thus , recombinant chicken His delta SCF together with the SCF specific antibody provide valuable tools for studying the role of SCF and c kit receptor in development , growth and differentiation of avian haematopoietic cells . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Although c fms is homologous to c kit , which encodes the receptor for stem cell factor ( SCF ) , EML C 1 cells neither express c fms nor respond to macrophage colony stimulating factor ( M CSF ) , the ligand for c fms . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) and its receptor Kit regulate the proliferation and survival of early hematopoietic cell types as well as germ cells and melanocytes . ^^^ Thus , SCF and its receptor Kit are expressed in human endometrium and placental tissues during pregnancy , and the pattern of receptor / ligand expression suggests that endometrial and placental villous core SCF may have a paracrine effect on trophoblast through the receptor Kit . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Here we describe the expression and functional role of the proto oncogene c kit and of its specific ligand stem cell factor ( SCF ) on human osteoclasts , osteoblasts , and stromal cells derived from different sources . ^^^ Proliferation of FLG 29 . 1 preosteoclast cells was stimulated by exogenous SCF , indicating that c kit was capable of transducing growth signals . ^^^ Finally , in vitro adhesion of FLG 29 . 1 cells to primary bone marrow stromal cells , GCT derived stromal cells ( C 433 ) , and Saos 2 osteoblast cells was significantly inhibited by an excess of soluble SCF or by monoclonal antibodies recognizing SCF binding sites on the c kit receptor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The transcripts of c mpl , the receptor of TPO , was not detected in all SCLC cell lines by RT PCR analysis , while those of c kit , the receptor of SCF , were detected in five of eight SCLC cell lines . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The expression and production of c kit and its ligand , stem cell factor ( SCF ) , in cord blood and neonates were studied . ^^^ SCF and c kit levels in placental tissue homogenates and the culture media of decidual cells and trophoblasts were low . ^^^ To determine the sites of high SCF and c kit production in cord blood and in early neonates . ^^^ SCF and c kit mRNA expression was analysed in various tissues by polymerase chain reaction . ^^^ Our findings suggest that endothelial cells have an important role in the maintenance and proliferation of progenitor cells in neonatal blood via the interaction of c kit and SCF with other factors . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The percentage of cells that expressed c Kit , the SCF receptor , did not appear to differ between the two sources of CFU C . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
RESULTS : The peripheral blood contained 1 % to 3 % nonmetachromatic mononuclear cells with eccentric nuclei that expressed the mast cell proteases , tryptase and carboxypeptidase A , along with c kit , stem cell factor ( SCF ) , and high affinity IgE receptor ( Fc epsilon RI ) , but not chymase . ^^^ Similar mononuclear cells colocalized in the spleen and lymph nodes with mature , metachromatic mast cells that expressed tryptase , chymase , carboxypeptidase A , c kit , SCF , and Fc epsilon RI . ^^^ CONCLUSIONS : The peripheral blood of a patient with aggressive systemic mastocytosis contained immature mononuclear cells of the mast cell lineage that express c kit , SCF , tryptase , carboxypeptidase A , and Fc epsilon RI . ^^^ The presence of SCF on the surface membrane of the circulating , highly immature mast cells suggests an autocrine regulation of the c kit SCF interaction . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Between 60 and 70 % of the CD34+ cells expressed the stem cell factor ( SCF ) receptor ( c KIT , CD 117 ) and there were no differences between BM HA and BM CML patients . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) is a noncovalent homodimeric cytokine that exhibits profound biological function in the early stages of hematopoiesis by binding to a cell surface tyrosine kinase receptor that is encoded by the c Kit proto oncogene . ^^^ The results obtained from a combined implementation of homology based molecular modeling and computational simulations in the study of species specific SCF / c Kit interactions are reported . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In the mouse , the Kit receptor and its ligand , the stem cell factor ( SCF ) , are encoded at the W / Kit and Steel loci , respectively . ^^^ The Kit / SCF transduction pathway is involved in promoting cellular migration , proliferation and / or survival of melanoblasts , hematopoietic progenitors and primordial germ cells . ^^^ Furthermore , a functional Kit / SCF pathway is required for the development of interstitial cells of Cajal ( ICC ) in the small intestine . ^^^ Nevertheless , we assume that the Kit / SCF pathway could be involved in the growth of transformed endothelial , epithelial and endocrine cells . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mice harboring the `` white spotting ' ' ( W ) locus have abnormalities in hematopoiesis due to one of various mutations of the c kit proto oncogene , which encodes the stem cell factor ( SCF ) receptor . ^^^ These results suggest that neither c kit mutations nor decreased soluble SCF levels are commonly involved in the pathogenesis of acquired AA . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
By using the growth factor dependent M 07e cell line , we found that both c Mpl and c Kit ligands , namely : SCF and TPO , induce c Cbl tyrosine phosphorylation . ^^^ In vitro experiments showed that the stable Grb2 / c Cbl complex interacts , through the Grb 2 SH2 domain , with the SCF activated c Kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Exposure of c KIT positive melanoma cells in vitro and in vivo to stem cell factor ( SCF ) , the ligand for c KIT , triggered apoptosis of these cells but not of c KIT negative melanoma cells or normal melanocytes . ^^^ Since SCF is produced by keratinocytes and other dermal cells in the skin , these results suggest that the loss of c KIT receptor expression may allow malignant melanoma cells to escape SCF / c KIT mediated apoptosis , hence contributing to tumor growth and eventually metastasis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In particular , Tpo potently synergized with the ligands for c kit ( stem cell factor [ SCF ] ) and flt 3 ( FL ) to stimulate an increase in the number and size of clones formed from Lin Sca 1+ progenitors . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We have characterized the cellular localization of stem cell factor ( SCF ) and c kit receptor ( c kitR ) in the adult mouse nervous system in situ and in culture by using immunocytochemistry . ^^^ Northern blot analysis confirmed the expression of mRNAs of c kit and SCF in cultured neurons , astroglia , and microglia . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In fractionated BM , the majority ( > 99 % ) of CFU O were in the c kit positive population ; however , a specific antibody to SCF did not affect O CSF induced TRAPase+ colony formation , suggesting the proliferation and differentiation of osteoclast progenitors are independent of c kit SCF interactions . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The relative binding of SCF to suspensions of tumour cells , as assessed by flow cytometry , correlated with the intensity of immunolabelling for SCFR in sections of the same tumours , suggesting variability in SCFR expression between tumours . ^^^ This relatively common malignancy of the dog would seem to present an opportunity for the investigation of the potential role of the SCF / SCFR pathway in the development of spontaneous malignancies of mast cells . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Signaling through c Kit / stem cell factor ( SCF ) is crucial for normal development of erythroid and myeloid hematopoietic precursors and of melanocytes and germ cells . ^^^ Analysis of radiation bone marrow chimeras demonstrated that the dysregulation required either disruption of stromal cell SCF or IEL c Kit and showed that the effect on IEL or their precursors was not due to other changes in the intestinal microenvironment . ^^^ Possible mechanisms for the loss of proliferative regulation in gut T cells in c Kit / SCF deficiency are discussed . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
While c kit is activated by SCF ; gp 130 , in cells which do not express sufficient amounts of IL 6R , can be activated by the complex of soluble IL 6R ( sIL 6R ) and IL 6 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
MCF 7 , which does not express c kit but does express its ligand , stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We have studied binding of the dimeric cytokine stem cell factor ( SCF ) to the extracellular domain of its receptor Kit , which is a receptor tyrosine kinase similar to the receptors for platelet derived growth factor and colony stimulating factor 1 . ^^^ Calorimetric studies show that one SCF dimer binds simultaneously to two molecules of the Kit extracellular domain . ^^^ It has been proposed that SCF induced Kit dimerization proceeds via a conformational change that exposes a key receptor dimerization site in the fourth of the five immunoglobulin ( Ig ) like domains in Kit . ^^^ We show that a form of Kit containing just the first three Ig domains ( Kit 123 ) binds to SCF with precisely the same thermodynamic parameters as does Kit 12345 . ^^^ Analytical ultracentrifugation , light scattering , and gel filtration show that Kit 123 dimerizes upon SCF binding in a manner indistinguishable from that seen with Kit 12345 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The results suggest a correlation between dimerization affinity and biological activity , consistent with a model in which SCF dimerization mediates dimerization of its receptor , Kit , and subsequent signal transduction . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
However , only Epo R F7Y479 is capable of supporting erythroid colony formation when expressed in ( Epo R ) / fetal liver cells , indicating that Y 464 either can not send a differentiation signal or fails to respond to SCF / KIT activation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
F . ( 1995 ) Nature 377 , 242 246 ) documents the interaction of the erythropoietin ( EPO ) receptor ( EPOR ) with the stem cell factor ( SCF ) receptor ( c KIT ) and suggests that SCF acts through the EPOR . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
All antibodies except the Genzyme Mab effectively blocked SCF binding to c Kit expressing cells , and were strongly inhibitory in an assay of in vitro haemopoiesis which is believed to depend on adhesive interactions , as well as the `` classical ' cytokine receptor interaction , mediated by SCF binding to c Kit . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Coexpression of flt 3 ligand / flt 3 and SCF / c kit signal transduction system in bile duct ligated SI and W mice . ^^^ Stem cell factor ( SCF ) and its receptor c kit constitute an important signal transduction system regulating cell growth and differentiation in hematopoiesis , gametogenesis , and melanogenesis . ^^^ Recently , we have demonstrated that both SCF and c kit are expressed in the bile duct epithelial cells of the rat liver and are highly up regulated during activation of the normally dormant hepatic stem cell compartment . ^^^ In the present study , we used sl / sld and w / wv mice , which have mutation of either SCF or c kit , to study the possible involvement of the SCF / c kit system in the bile duct proliferation . ^^^ The transcripts for both SCF and c kit were clearly increased after bile duct ligation in both control and mutant mice . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
It has been confirmed that the receptor protein encoded by the c kit proto oncogene is expressed by cells of the hematopoietic , gonadal , pigment , and mast cell lineages and that its ligand , stem cell factor ( SCF ) , is mainly expressed in their microenvironment . ^^^ These results indicate the existence of mast cell SMC interaction and of an autocrine loop of c kit and its ligand on the surface of EC , suggesting that the interaction between c kit protein and SCF may play an important role in metabolism of arterial wall and in the pathogenesis of atherosclerosis in the arterial intima . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We now report that activation of mouse mast cells via c kit , the receptor for the pleiotropic survival / growth factor , stem cell factor ( SCF ) , can induce the release of IL 6 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The ligand for c kit receptor is stem cell factor ( SCF ) , which is the principal growth factor for mast cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Blocking mAbs against the c kit receptor have no effect on PMVEC CM induced CD34+ cell proliferation at titres that completely suppress SCF induced proliferation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
SCF and c kit in mastocytosis a Pandora ' s box holding more theories than proven facts . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To determine if Kit signal transduction activated latent DNA binding factors , including STAT 5 , we performed electrophoretic mobility shift assays on stem cell factor ( SCF ) stimulated mouse bone marrow derived mast cells ( BMMCs ) . ^^^ These data indicate that SCF stimulation of Kit leads to activation of STAT 5 DNA binding with kinetics distinct from Epo mediated stimulation . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Although no significant increase in the expression of stem cell factor ( SCF ) c kit , hepatocyte growth factor ( HGF ) , and transforming growth factor alpha ( TGF alpha ) was observed 7 days after bile duct ligation in adult rats , the expression of all these growth factors was increased in bile duct ligated rats up to 5 weeks of age . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The earliest B cell progenitors are bound to the stromal cell surface by adhesive interactions through cell surface molecules to promote the binding of c kit to stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Identification of a polymorphism in the transmembrane domain of the protooncogene c kit in healthy subjects . c Kit is the receptor for stem cell factor ( SCF ) and is found on hematopoietic stem cells , mast cells , melanocytes , and germ cells . ^^^ Aggregation of c Kit by SCF regulates cell proliferation , differentiation , and survival . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The product of the protooncogene c kit is the receptor for the hematopoietic cytokine stem cell factor ( SCF ) . ^^^ C kit is expressed on leukemic cells of the erythroid , myeloid , and mast cell lineage and SCF has a proliferative effect on some of these cells . ^^^ The role of SCF and c kit in lymphoid malignancies is much less clear . ^^^ C kit is expressed in normal lymphopoiesis and its ligand SCF synergizes with IL 7 to enhance the proliferation of B and T cell progenitors . ^^^ Almost all of the lymphoblastic cells expressing c kit protein responded to recombinant human ( rh ) SCF with a downregulation of c kit receptors . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
SCF is a ligand for c kit receptor with tyrosine kinase . ^^^ We review the literature regarding the role of SCF / c kit system in the oncogenesis of leukemia and MPD , and then discuss the significance of our finding in the context of growth advantage of the mutated clones over the normal clones . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We studied the effect of human flt3 / flk2 ligand ( FL ) on the proliferation and differentiation of purified CD34+ blood progenitors which express different levels of c kit protein in clonal cell culture in comparison with that of stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Coexpression of the Kit receptor tyrosine kinase and its ligand , stem cell factor ( SCF ) , occurs in a high proportion of small cell lung cancers ( SCLCs ) and drives an autocrine loop that enhances proliferation . ^^^ To determine whether this autocrine loop affects apoptosis , SCLC cells expressing only SCF or both SCF and Kit were deprived of growth factors for 72 h and the relative number of cells undergoing apoptosis was assessed using nuclear DNA content and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling assays . ^^^ Coexpression of SCF and Kit inhibited apoptosis ; apoptosis could , in turn , be enhanced by the addition of the quinoxaline tyrosine kinase inhibitors , which are specific antagonists of the platelet derived growth factor receptor and Kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To test this latter possibility , a chimeric receptor containing the extracellular domain of c kit ( the stem cell factor ( SCF ) receptor ) and the cytoplasmic and transmembrane domains of the IL 4R alpha chain was generated . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We evaluated mature peripheral blood eosinophils for their expression of the surface tyrosine kinase , c kit , the receptor for the stromal cell derived cytokine , stem cell factor ( SCF ) . ^^^ Cell adhesion to FN 40 was completely inhibited with antibodies against the alpha 4 and beta 1 integrin subunits , revealing that the SCF / c kit adhesion effect was mediated by a single integrin heterodimer , very late antigen 4 ( VLA 4 ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Because mast cells express the KIT protein , the receptor for SCF , and respond to SCF by proliferation and degranulation , this observation identifies a possible feedback loop in which chymase released from mast cell secretory granules may solubilize SCF bound to the membrane of surrounding stromal cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The receptor for SCF is c Kit , a receptor tyrosine kinase . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
However , GATA 1 expression did result in a selective and marked inhibition in mitogenic responsiveness to SCF and to a decrease in c kit transcript expression . ^^^ These studies of SCF and Epo signaling in FDC 2 wt ER cells serve to functionally map the ERB 1 region as a c kit interactive domain , suggest that Pim 1 might contribute to SCF and Epo mitogenic synergy and support the notion that SCF and Epo may act in opposing ways during red cell differentiation . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We have previously shown that stem cell factor ( SCF ) , the ligand to the c kit tyrosine kinase receptor , enhances IL 2 induced NK cell proliferation and differentiation in vitro . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Analysis of mice with naturally occurring mutation in the genes encoding the tyrosine kinase receptor protein , c kit , and its ligand , stem cell factor ( SCF ) , has implicated these proteins in testicular development and function because of the phenotype of these mice of reduced or absent fertility . ^^^ This review summarises the current knowledge regarding functions of c kit and SCF in the testis and discusses the implications of information gleaned from other biological systems . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Interestingly enough c kit , the receptor of SCF , is coexpressed on HUVEC , suggesting an autoregulatory mechanism . ^^^ Binding experiments with 125I SCF were performed to study the c kit receptor expression on HUVEC . ^^^ Our data showed that , in contrary to the previously reported upregulation of SCF mRNA expression , stimulation with Yersinia enterocolitica or with Neisseria meningitidis led to a significant time dependent downregulation of c kit mRNA within 3 hours . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We also show that the SCF / c kit interaction can be substituted by an unknown mechanism . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Interaction of stem cell factor ( SCF ) , a haematopoietic growth factor , with the receptor tyrosine kinase c kit leads to autophosphorylation of c kit as well as tyrosine phosphorylation of various substrates . ^^^ We recently found that the Janus kinase Jak 2 associates with c kit and that SCF induces rapid and transient phosphorylation of Jak 2 . ^^^ Phosphorylated c kit co immunoprecipitates with Stat 1 within 1 min of SCF stimulation of the human cell line MO7e . ^^^ SCF induced phosphorylation of Jak 2 and Stat 1 was also observed in murine 3T3 fibroblasts stably transfected with full length human c kit receptor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) is a glycoprotein growth factor produced by marrow stromal cells that acts after binding to its specific surface receptor , which is the protein encoded by the protooncogene c kit . ^^^ SCF and its c kit receptor are expressed by some tumor cells , including myeloid leukemia , breast carcinoma , small cell lung carcinoma , melanoma , gynecological tumors , and testicular germ cell tumors . ^^^ All lines expressed mRNA for c kit and c kit protein at low intensity as measured by flow cytometry , and secreted SCF in medium culture as shown by ELISA . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Exposure of c KIT positive melanoma cells in vitro and in vivo to stem cell factor ( SCF ) , the ligand for c KIT , triggered apoptosis of these cells but not of normal melanocytes . ^^^ These results suggest that the loss of c KIT receptor may allow malignant melanoma cells to escape SCF / c KIT mediated apoptosis , thus contributing to tumor growth and eventually metastasis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
WTKit cells were able to grow in the presence of the human ligand for Kit , stem cell factor ( SCF ) , but displayed reduced growth and clonogenic potential in either SCF or GM CSF compared with the parental cells in GM CSF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , the ligand for the c kit receptor , is produced mainly by marrow stromal cells and seems to reflect the haemopoietic function of bone marrow stroma . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Following testicular injury by 2 , 5 HD , stem cell factor ( SCF ) , a Sertoli cell derived growth factor that binds the c kit receptor on spermatogonia , is altered in its expression , changing from predominantly membrane SCF to predominantly soluble SCF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The receptor encoded by the W ( c kit ) locus is expressed on the membrane of mouse primordial germ cells , whereas its ligand termed stem cell factor ( SCF ) , encoded by the Sl locus , is expressed on the membrane of somatic cells associated with both the primordial germ cell migratory pathways and homing sites . ^^^ Using an in vitro short time assay which allows a quantitative measure of adhesion between cells , in the present paper we show that SCF / c kit interaction can modulate primordial germ cell adhesion to somatic cells . ^^^ We report that the adhesiveness of 11 . 5 dpc primordial germ cells to four types of somatic cells in culture ( TM 4 cells , STO fibroblasts , bone marrow stromal cells and gonadal somatic cells ) is significantly reduced by antibodies directed against c kit receptor or SCF , as well by soluble SCF . ^^^ This SCF / c kit mediated adhesion seems independent of SCF induced tyrosine autophosphorylation of c kit receptor . ^^^ These results demonstrate that SCF / c kit interaction contributes to the adhesion of primordial germ cells to somatic cells in culture and suggest that the role played by SCF in promoting survival , proliferation and migration of these cells in vitro and in vivo , demonstrated by several studies , might depend on the ability of the membrane bound form of this cytokine to directly mediate primordial germ cell adhesion to the surrounding somatic cells . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
SCF alone tyrosine phosphorylated several bands including the 145 kDa subunit of c kit . ^^^ The combination of SCF and GM CSF resulted in a synergistic increase in tyrosine phosphorylation of the 145 kDa subunit of c kit . ^^^ Thus , a synergistic increase in tyrosine phosphorylation of the 145 kDa subunit of c kit may be one possible mechanism underlying the cooperation of SCF and GM CSF on the HML 2 cell growth . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mutations of the receptor tyrosine kinase c kit or its ligand stem cell factor ( SCF ) , which is encoded as a soluble and membrane associated protein by the Steel gene in mice , lead to deficiencies of germ cells , melanocytes , and hematopoiesis , including the erythroid lineage . ^^^ Bone marrow derived stromal cells expressing only a membrane restricted ( MR ) isoform of SCF induced an elevated and sustained tyrosine phosphorylation of both c kit and erythropoietin receptor ( EPO R ) and significantly greater proliferation of an erythrocytic progenitor cell line compared with stromal cells expressing soluble SCF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) binding to the c kit receptor triggers homodimerization and intermolecular tyrosine phosphorylation of the c kit receptor , thus initiating signal transduction . ^^^ Prior biochemical studies of c kit receptor dimerization have mainly used affinity cross linking techniques , which are beset with problems including low efficiency of cross linking and the usual requirement for radiolabeled SCF to detect the cross linked complex . ^^^ We used the fluorescence resonance energy transfer ( FRET ) technique to examine the effects of SCF and other hematopoietic cytokines on c kit receptor dimerization . ^^^ The ability of SCF to induce c kit receptor dimerization was assessed by flow cytometric analysis of FRET between the donor fluorochrome FITC and the acceptor fluorochrome Cy 3 . ^^^ SCF induced a dose dependent increase in c kit receptor dimerization that correlated well with the concentrations of SCF required to stimulate cell proliferation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Expression of the c Kit receptor and its ligand SCF in non small cell lung carcinomas . ^^^ Increasing experimental evidence indicates that stem cell factor ( SCF ) and its cognate receptor c Kit may participate in the growth control of various solid human malignancies . ^^^ The results of an immunohistochemical analysis demonstrated that c Kit / SCF are expressed by 30 % / 58 % of adenocarcinomas , 15 % / 37 % of squamous cell carcinomas and by 40 % / 30 % of undifferentiated carcinomas respectively . ^^^ Since biologically active SCF is physiologically present in the bloodstream , our data indicate that SCF is available to c kit expressing NSCLC cells via autocrine , paracrine or endocrine mechanisms . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Double staining with toluidine blue and immunofluorescence using monoclonal antibodies ( mAbs ) revealed expression of stem cell factor ( SCF ) receptor ( c kit / CD117 ) , CD 9 , CD 29 , CD 33 , CD 43 , CD 44 , CD 54 , and CD 63 on HRMCs . ^^^ Ligand induced activation of HRMCs through immunoglobulin ( Ig ) E R or SCF R ( c kit ) resulted in histamine secretion ( control : < 10 % ; alphaIgE , 1 microg / mL : 50 . 12 + / 5 . 18 % ; rhSCF , 100 ng / mL : 29 . 24 + / 22 . 39 ) , whereas recombinant C5a , erythropoietin ( EPO ) , IL 1 through 10 , and GM CSF exerted no effects . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Viral incorporation of the SCF chimeric envelopes was demonstrated by immunoblotting of pelleted virions and their specific attachment to Kit receptors was demonstrated by flow cytometry . ^^^ Gene transfer studies showed that when SCF was displayed on an amphotropic envelope , the infectivity of the SCF displaying vectors was selectively inhibited on Kit expressing cells , but could be restored by adding soluble SCF to block the Kit receptors or by cleaving the displayed SCF domain from the vector particles with factor Xa protease . ^^^ When EGFR positive cancer cells and Kit positive hematopoietic cells were mixed and exposed to the different engineered vector particles , the cancer cells were selectively transduced by the SCF displaying vector and the hematopoietic cells were selectively transduced by the EGF displaying vector . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The stem cell factor ( SCF ) c kit receptor interaction plays a critical role in the development and survival of mast cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
OBJECTIVE : To determine expression of c kit and stem cell factor ( SCF ) mRNA in liver specimens from healthy adult dogs and to investigate whether differentiation of mast cells in the liver of dogs is supported by the c kit receptor tyrosine kinase / SCF system . ^^^ Magnitudes of c kit and SCF mRNA expression in liver samples was determined by means of northern blot analysis , using probes based on canine sequences . ^^^ To determine which cells were expressing c kit and SCF mRNA , in situ hybridization was performed . ^^^ RESULTS : Expression of c kit and SCF mRNA in liver samples was weak but appreciable . ^^^ In situ hybridization revealed that c kit and SCF mRNA expression was restricted to mast cells and plasma cells , respectively . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
RESULTS : In both groups of patients ( RA and OA ) , SyMC were found to react with MAb to IgE , SCF receptor ( c kit , CD 117 ) , as well as CD antigens likewise expressed in lung MC ( CD 9 , CD 29 , CD 33 , CD 43 , CD 44 , CD 45 ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Expression of protooncogene c kit and its ligand stem cell factor ( SCF ) in gastric carcinoma cell lines . ^^^ We examined 13 human gastric carcinoma cell lines for the expression of both c kit and stem cell factor ( SCF ) . ^^^ Northern blot analysis showed coexpression of both c kit and SCF in ECC 12 and expression of SCF in five other ( MKN 74 , MKN 1 OKAJIMA , KATOIII , and TMK 1 ) cell lines . ^^^ SCF stimulated both tyrosine phosphorylation of c kit and growth of ECC 12 , whereas it did not stimulate those of GCIY . ^^^ The present study suggests that c kit / SCF system might play an important role in the carcinogenesis and tumor growth of ECC 12 and that the truncated form of c kit in GCIY might not be associated with malignant transformation . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
During development , mice with mutations of stem cell factor ( SCF ) or its receptor c kit exhibit defects in melanogenesis , as well as hematopoiesis and gonadogenesis . ^^^ Consequently , accumulating evidence suggests that the c kit / SCF system plays a crucial role in all of these processes and in tumors which derive from them . ^^^ Especially in neuroblastoma ( infant tumors of neuroectoderm crest derivation such as melanocytes ) it would appear that an autocrine loop exists between c kit and SCF , and that the functional block of the c kit receptors with monoclonal antibodies ( MoAbs ) results in a significant decrease in cellular proliferation . ^^^ We studied the expression and role of c kit and SCF in cell lines of soft tissue sarcoma of neuroectodermic origin , such as Ewing ' s sarcoma ( ES ) and peripheral neuro ectodermal tumors ( PNET ) . ^^^ These results indicate that ES and PNET express c kit and its ligand SCF and that SCF is capable of protecting the tumor cells against apoptosis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The c kit protooncogene receptor and its ligand stem cell factor ( SCF ) regulate the proliferation and survival of germ cells as well as hematopoietic cells and melanocytes . ^^^ In adult rodent ovary , c kit and SCF play important roles in follicular development . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The growth and differentiation of mast cells and melanocytes require stem cell factor ( SCF ) , the ligand for the kit receptor tyrosine kinase . ^^^ Abnormalities of the SCF kit signaling pathway , with increased local concentrations of soluble SCF , have been implicated in the pathogenesis of the human disease cutaneous mastocytosis , but have not yet been shown to play a causal role . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The c PTIO promoted colony formation by erythropoietin and either interleukin 6 ( IL 6 ) or the c kit ligand / stem cell factor ( SCF ) in a methylcellulose culture , where the number of colonies increased 2 . 2 fold and 1 . 7 fold , respectively . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In order to explore the potential existence of human mast cell growth factors other than stem cell factor ( SCF ) , we have compared SCF to L cell fibroblast supernatants ( LCS ) during in vitro mast cell differentiation , using human leukaemic mast cells ( HMC 1 cells ) which contain a gain of function mutated SCF receptor ( c Kit ) as model . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The c kit encoded receptor protein tyrosine kinase for stem cell factor ( Kit / SCF R ) is essential for the development of cells within the hematopoietic , melanogenic and gametogenic lineages . ^^^ A Kit / SCF R gain of function mutant that has increased mitogenic and PI 3 kinase activation potential , due to the absence of the two protein kinase C negative feedback phosphorylation site , enhanced both Akt activation and Bad phosphorylation and also resulted in increased cell survival . ^^^ Such a mechanism may account for how deregulated PI 3 kinase activity and naturally occurring gain of function point mutants of Kit / SCF R lead to cellular transformation and fatal malignancies in man . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We compared the effect of human flt 3 ligand ( FL ) and stem cell factor ( SCF ) on cord blood ( CB ) derived CD34+ cells expressing different levels of flt 3 or c kit tyrosine kinase ( TK ) receptor in clonal cell culture . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We reported previously that stem cell factor ( SCF ) is produced mainly by neurons and that its receptor ( c kitR ) , encoded by the protooncogene c kit , is expressed in microglia , suggesting that SCF / c kitR signaling may be involved in neuron microglia interactions . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Furthermore , the ligand for c KIT , SCF , induces apoptosis in human melanoma cells expressing c KIT under both in vitro and in vivo conditions . ^^^ These results indicate that the expression of c KIT is highly regulated by AP 2 and that enforced AP 2 expression suppresses tumorigenicity and metastatic potential of human melanoma cells , possibly through c KIT transactivation and SCF induced apoptosis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem Cell Factor ( SCF ) , the ligand for the c kit proto oncogene , has been shown to synergistically interact with other cytokines , enhancing the responsiveness of haemopoietic precursors . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The interaction of SCF and Kit affects early hematopoietic progenitors , as well as gametocytes , melanocytes , and mast cells . ^^^ Upon binding of SCF the Kit undergoes dimerization and transphosphorylation . ^^^ Circular dichroism ( CD ) , intrinsic fluorescence , and Fourier transform infrared ( FTIR ) spectroscopy were used for conformational analyses of free SCF , soluble Kit ( sKit ) , and the complex . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Since HPC express c kit on their surface , the SCF produced by HUVEC plays an important role in the hematopoiesis of CB . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Acute myeloid leukaemia ( AML ) cells express the SCF receptor c kit ( CD 117 ) on their cell surface and demonstrate enhanced adhesion to fibronectin ( FN ) following exposure to stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Determination of the action of SCF , however , requires localization of its receptor , c kit ; therefore , the objectives of the present study were to identify and localize c kit within corpora lutea . ^^^ Given the temporal regulation of c kit expression within the corpus luteum , these data suggest that luteal SCF may act locally . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The interaction between p 145 ( c KIT ) and p 210 ( bcr abl ) in transduced cell lines , and the selective outgrowth of normal progenitors during long term culture of chronic myeloid leukemia ( CML ) cells on stroma deficient in stem cell factor ( SCF ) suggests that the response of CML cells to SCF may be abnormal . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The ligands for the protein tyrosine kinase receptors c KIT and FLT3 / FLK2 , stem cell factor ( SCF ) , and FL are produced by BM stromal cells and are known to affect several facets of hematopoiesis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We therefore investigated the role of the SCF receptor , the protooncogene c kit , in the pathogenesis of pediatric T lineage malignancies . ^^^ Expression and regulation of c kit in cells from children with non Hodgkin ' s lymphoma ( T NHL ) or acute lymphoblastic leukemia ( T ALL ) and the proliferative effect of SCF on these cells were examined in seven cell lines and 21 biopsy tumor cell preparations . ^^^ Inducibility of c kit receptors by SCF , IL 1beta , IL 2 , IL 7 , TGF beta , TNF alpha , PMA or calcium ionophore A 23187 was studied by flow cytometry ( FCM ) . ^^^ Downregulation of c kit occurred in the presence of SCF or PMA . ^^^ SCF caused a downregulation of c kit receptors in eight of nine , and a proliferative response in three of 11 c kit positive T lymphoblastic cell preparations . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Moreover , no effect of PDGF was observed when BMMC were prepared from W / W ( 5 ) mice that lack functional c kit as the SCF receptor or when 3T3 fibroblasts were prepared from Sl / Sl ( d ) mice that lack membrane bound SCF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Furthermore , exposure of c KIT positive melanoma cells in vitro and in vivo to stem cell factor ( SCF ) , the ligand for c KIT , triggered apoptosis of these cells but not of normal melanocytes . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Serum stem cell factor ( SCF ) and soluble KIT ( sKIT ) levels were estimated in patients with chronic renal failure ( CRF ) and anaemia , and compared with clinical parameters of blood cells and renal function . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Coligation of SCFR augmented Fc epsilonRI mediated activation of MAP kinases , especially JNK activation , and SCF augmented Fc epsilonRI mediated TNF alpha production in MC / 9 cells , although SCF alone did not induce TNF alpha production . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To determine the significance of stem cell factor ( SCF ) and soluble KIT ( sKIT ) in the serum of patients with systemic lupus erythematosus ( SLE ) , levels of SCF and sKIT in patients with SLE were estimated , and their correlations with clinical parameters were examined . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Lck associates with and is activated by Kit in a small cell lung cancer cell line : inhibition of SCF mediated growth by the Src family kinase inhibitor PP 1 . ^^^ At least 70 % of small cell lung cancers ( SCLCs ) express the Kit receptor tyrosine kinase and its ligand , stem cell factor ( SCF ) . ^^^ SCF treatment of the H 526 cell line induced a physical association between Kit and Lck that , in vitro , was dependent on phosphorylation of the juxtamembrane domain of Kit . ^^^ Stimulation of Kit with recombinant SCF resulted in a rapid 3 6 fold increase in the specific activity of Lck , which was similar in magnitude to the activation of Lck resulting from the cross linking of the T cell receptor complex of Jurkat cells . ^^^ These data demonstrate that , in the H 526 SCLC cell line , Lck and , possibly , Yes are downstream of Kit in a signal transduction pathway ; the inhibition by PP 1 of SCF mediated proliferation and inhibition of apoptosis suggests that Src family kinases are intermediates in the signaling pathways that regulate these processes . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Four of 5 rhabdomyosarcoma cell lines expressed the mRNA for SCF receptor c kit , while the 5th cell line became weakly positive for c kit mRNA only after stimulation with retinoic acid . ^^^ Addition of anti c kit and anti SCF blocking antibodies , or of exogenous SCF did not alter the in vitro growth ability of rhabdomyosarcoma cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
METHODS : Mutant c kit cDNA was transfected into an interleukin 3 ( IL 3 ) dependent Ba / F3 murine lymphoid cell line , and the magnitude of autophosphorylation of the mutant KIT was examined with or without stem cell factor ( SCF ) , a ligand of KIT . ^^^ RESULTS : The mutant KIT exhibited constitutive phosphorylation and strong kinase activity without SCF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mast cells are reported to differ from other cells of the hematopoietic lineage in that as mature cells , they retain the c kit receptor , and are thus capable of responding to the stem cell factor ( SCF ) ligand . ^^^ The mechanisms whereby brain mast cells might survive in the absence of SCF c kit signaling are discussed . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
SCF / IL 6 / IL 10 transiently elicited a cell subpopulation with the phenotype ( c kit ( high ) Thy 1 ( low ) ) of fetal blood promastocytes at 3 wk of culture that progressed within 1 wk to FcepsilonRI bearing PrMC , designated PrMCTriad . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
SCF only down regulated expression of c Kit with a fold decrease of 4 . 6 + / 0 . 9 for GMFI ( P=0 . 043 ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Expression of stem cell factor ( SCF ) and SCF receptor ( c kit ) in synovial membrane in arthritis : correlation with synovial mast cell hyperplasia and inflammation . ^^^ OBJECTIVE : Stem cell factor ( SCF ) , the ligand for the SCF receptor ( c kit ) expressed on precursors and mature mast cells ( MC ) , is a major agonist for human MC ( e . g . , SCF induces MC development , chemotaxis , activation , proliferation of MC precursors , mediates MC adhesion , and changes MC releasability ) . ^^^ We investigated expression of SCF and c kit in synovial membrane with particular reference to the mechanism of local MC hyperplasia and inflammation in arthritis . ^^^ METHODS : We conducted single and double labeling immunohistochemistry ( ABC , APAAP , indirect immunofluorescence techniques ) with antibodies to SCF , c kit , MC tryptase , Ki 67 antigen ( marker for proliferating cells ) , and CD 68 ( monocyte / macrophage marker ) . ^^^ RESULTS : Synovial c kit expression was found to be restricted to MC , whereas SCF is detected in synovial lining cells , stromal fibroblasts , monocyte / macrophages , endothelial cells , and in vascular basement membranes . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Immunohistochemical analysis revealed the presence of SCF in the mesenchymal cells of the chorion , the trophoblast , and decidual stromal cells , whereas the SCF receptor , c kit , was detected in the trophoblast and decidual mononuclear leukocytes but not in decidual stromal cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Growth of TF 1S and TF 1 cells on stroma still requires interaction between c kit ( SCF receptor ) and its ligand SCF , because antibodies against c kit inhibit growth to less than 2 % . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Low SCF in HFSS patients is associated with elevated TGF beta , suggesting a regulatory role of the latter on either SCF release or c kit expression in progenitor cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The stem cell factor ( SCF : a ligand for c kit ) plays a central role in the growth of myelodysplastic ( MDS ) progenitor cells with leukemic type growth . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
A subsequent proliferative response to stem cell factor ( SCF ) , the ligand for the proto oncogene receptor tyrosine kinase c kit , is characterized by an initial maturation in immunophenotype and subsequent self renewal of cells ( SCF blasts ) without differentiation for at least 50 generations . ^^^ Data indicate a pivotal role for SCF / c kit interaction during antigenic maturation , self renewal , and apoptotic protection of these lineage restricted progenitors during non CSF mediated induction of differentiation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Binding of stem cell factor ( SCF ) to its receptor ( SCFR , c Kit ) on mast cells also induces increases in intrinsic tyrosine kinase activity and activation of PI 3 kinase . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Erythroid differentiation / maturation was induced in the highly purified PB CD 34 positive cells in the liquid suspension culture with interleukin 3 ( IL 3 ) , stem cell factor ( SCF ; a ligand for c kit ) and human erythropoietin ( EP ) ; 8 days of the culture generated progenitors equivalent to colony forming units erythroid ( CFU E ) and 12 days of the culture generated a population mainly consisting of polychromatophilic normoblasts . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The receptor for FL belongs to the class 3 family of receptor tyrosine kinases which also includes c kit , the receptor for stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Oval cells that develop in the rat 2 acetylaminofluorene / partial hepatectomy ( AAF / PH ) model express the c kit receptor tyrosine kinase ( KIT ) and its ligand , stem cell factor ( SCF ) . ^^^ We investigated the role of the SCF / KIT system in the development of oval cells using Ws / Ws rats , whose c kit kinase activity was severely impaired owing to a small deletion in the kinase domain . ^^^ The present results indicate that the signal transduction of the SCF / KIT system plays a crucial role in the development of oval cells , at least , in the rat AAF / PH model , and suggest that KIT mediated signal transduction plays only a small role in determining the phenotype and in the proliferative activity of oval cells . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The expression pattern of c fos , c jun , c kit and stem cell factor ( SCF ) has been investigated in developing human placenta using the highly sensitive technique of in situ reverse transcriptase polymerase chain reaction ( RT PCR ) . ^^^ Specific transcripts of all genes under study were observed in first trimester placenta sections . c fos , c jun , c kit and SCF transcripts were localized in cells of the villous stroma ; fos , jun and kit specific mRNAs were also found in endothelial cells ; fos , kit and SCF mRNAs were detected in villous trophoblast cells . ^^^ Localization of fos , jun , kit and SCF mRNA in human placenta throughout gestation using in situ RT PCR . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
However , the down regulation of c kit , which is associated with the mechanism of mobilization , was much greater in the PBPC mobilized by SCF and G CSF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Moreover , stromal cells express the MRNA for stem cell factor ( SCF ) and c kit ( the receptor for SCF ) was detected on CD 3 thymocytes but not on CD3+ thymocytes . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Previously it has been shown that c kit receptor ( c kitR ) and its ligand , stem cell factor ( SCF ) , are expressed in the central nervous system . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
IL 4 dose dependently enhanced the expression of c kit and high affinity receptor for IgE ( Fc ( epsilon ) RI ) on the cell surface of SCF cultured BM cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The cytokines macrophage colony stimulating factor ( M CSF ) and stem cell factor ( SCF ) bind to related members of this tyrosine kinase receptors family , c fms and c kit , respectively . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Previous findings indicate that the protein c KIT and its ligand , stem cell factor ( SCF ) play a crucial role in the development of melanocytes from their precursors in the embryonic neural crest cells . ^^^ In this study , to clarify the underlying mechanism , we studied whether ACK 2 induces apoptosis in c KIT positive melanoblasts , which appear in mouse neural crest cells cultured with SCF from 9 . 5 d old mouse embryos . ^^^ It was therefore demonstrated that apoptosis was induced in the SCF dependent c KIT positive melanocytes in vitro when the SCF / c KIT interaction was obstructed . ^^^ These findings elucidate the mechanism of the regulation of melanocyte development , and the survival and proliferation of these precursor cells , by SCF / c KIT interaction . . ^^^ With an in situ apoptosis detection kit , a significant increase in apoptosis was detected after the removal of SCF , which further increased with the addition of ACK 2 during SCF dependent periods . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) and its tyrosine kinase receptor , c Kit , play a crucial role in regulating migration and proliferation of melanoblasts , germ cells , and hemopoietic cell progenitors by activating a number of intracellular signaling molecules . ^^^ Here we report that SCF stimulation of myeloid cells or fibroblasts ectopically expressing c Kit induces physical association with and tyrosine phosphorylation of three signal transducers and activators of transcription ( STATs ) as follows : STAT1alpha , STAT5A , and STAT5B . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The Sl locus encodes stem cell factor ( SCF ) , which is the ligand of c kit , a receptor tyrosine kinase encoded by the W locus . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The stem cell factor ( SCF ) / c kit ligand / receptor system has been implicated in stem ( oval ) cell activation following liver injury in the rat . ^^^ The aim of this study was to determine the role of the SCF / c kit system in pediatric human liver during acute and chronic liver injury . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To elucidate the function of PTP RO in megakaryocytic cells and its potential involvement in the stem cell factor ( SCF ) / c Kit receptor pathway , COS 7 and 293 cells were cotransfected with the cDNAs of both the c Kit tyrosine kinase receptor and PTP RO . ^^^ PTP RO was found to be associated with the c Kit receptor in these transfected cells and the SCF / Kit ligand induced a rapid tyrosine phosphorylation of PTP RO . ^^^ The results demonstrated that PTP RO associates with c Kit but not with the tyrosine kinase receptor FGF R and that PTP RO is tyrosine phosphorylated after SCF stimulation of Mo7e and CMK cells . ^^^ Therefore , these data show that the novel tyrosine kinase phosphatase PTP RO is involved in megakaryocytopoiesis and that its function is mediated by the SCF / c Kit pathway . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The SCF / KIT pathway plays a critical role in the control of normal human melanocyte homeostasis . ^^^ During development , the interaction of stem cell factor ( SCF ) with its receptor , KIT , is critical for the survival of melanocytes . ^^^ In order to study the impact of this pathway on normal melanocyte homeostasis , human skin xenografts were treated with serial injections of recombinant human SCF or a KIT inhibitory antibody ( K44 . 2 ) . ^^^ On histologic evaluation , SCF injection increased , whereas KIT inhibition decreased the number , size , and dendricity of melanocytes . ^^^ These findings demonstrate that the SCF / KIT pathway remains critical in adult human skin , and that pharmacologic modulation of this single pathway can control cutaneous melanocyte homeostasis . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To accomplish this , a vector was constructed by covalently linking biotin molecules to recombinant adenovirus , followed by addition of the biotinylated ligand for the c Kit receptor , stem cell factor ( SCF ) , through an avidin bridge . ^^^ Substitution of biotinylated antibodies directed against c Kit , CD 34 ( binds L selectin ) , and CD 44 ( hyaluronate receptor ) receptors for biotinylated SCF resulted in 50 , 8 , and 260 fold increases in reporter gene expression , respectively , demonstrating that infection also could be redirected through antibody antigen interactions and through antigens other than growth factor receptors . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
After 4 wk of culture in the presence of recombinant stem cell factor ( SCF ) , interleukin ( IL ) 6 , and IL 10 , the cells were characterized as hPrMCs based upon their uniform surface expression of c kit and CD 13 , low level expression of FcinRIalpha , absence of CD 14 and CD 16 expression , and immunoreactivity for MC chymase in > 80 % , and about half were immunoreactive for tryptase and metachromatic with toluidine blue . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) binds the receptor tyrosine kinase c Kit and is critical in haemopoiesis . ^^^ Recently we found that the Src family member Lyn is highly expressed in SCF responsive cells , associates with c Kit and is activated within minutes of the addition of SCF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Kit receptor is a transmembrane tyrosine kinase that is the receptor for stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) and its receptor c kit regulate the proliferation and survival of germ cells and play an important role in follicular development . ^^^ However , little information is available on the role of SCF and c kit in the process of blastocyst implantation . ^^^ In the present study , we examined the expression of SCF and c kit mRNA in mouse embryos and in the stromal and epithelial cells of the uterine endometrium by reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ These results are consistent with the hypothesis that SCF derived from endometrial cells and the implanting embryo exerts paracrine and / or autocrine action on the process of implantation by stimulating trophoblast outgrowth through its receptor c kit . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Kit is activated by stem cell factor ( SCF ) , but the source of SCF in the bowel wall is unclear and controversy exists about whether enteric neurons express the SCF required for ICC development . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) exerts its biological effects by binding to a specific receptor , the tyrosine kinase c Kit , which is expressed on the cell surface . ^^^ Treatment of the SCF responsive human hematopoietic cell line MO7e with the inhibitor of Src family kinases PP 1 blocked SCF induced capping of c Kit and internalization of c Kit . c Kit was able to associate with clathrin in the presence of PP 1 , suggesting that entry of c Kit into clathrin coated pits occurs independently of Src family kinases . ^^^ SCF induced internalization of c Kit was also diminished in the D 33 3 lymphoid cell line in which expression of Lyn kinase was disrupted by homologous recombination . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Activating mutations in c Kit , the receptor for Stem Cell Factor ( SCF ) , have been identified in dysplasias and leukaemias of the mast cell lineage and have been shown to contribute to transformation in model systems . ^^^ Early myeloid cells also normally express c Kit and their survival , proliferation and differentiation is promoted by SCF . ^^^ We have found that mutant c Kit ( and normal c Kit in the presence of SCF ) provides a strong differentiation stimulus in normal and immortalised murine early myeloid cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The local production of stem cell factor ( SCF ) may be an important mechanism for regulating proliferation , differentiation , and migration of various cells bearing c kit receptors , and might be susceptible to the cytokines that serve in inflammation and tissue repair . ^^^ Our results suggest that TGF beta 1 and bFGF potentially modulate the biological function of cells bearing c kit receptors through the modulation of SCF production in fibroblasts . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Intestinal myofibroblasts are activated and proliferate in response to various growth factors , particularly the platelet derived growth factor ( PDGF ) family , which includes PDGF BB and stem cell factor ( SCF ) , through expression of PDGF receptors and the SCF receptor c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The combination of hGM CSF and SCF was effective on enriched , Lin ( ) Sca 1 ( + ) c kit ( + ) progenitors and increased either mixed colony forming unit or cobblestone area forming cells . ^^^ When Lin ( ) Sca 1 ( + ) c kit ( + ) CD 34 ( ) further enriched progenitors were clone sorted and individually incubated in the presence of SCF , hGM CSF stimulated a larger number of cells than did IL 6 , IL 6 and soluble IL 6 receptor ( IL 6R ) , or IL 11 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The receptor tyrosine kinase c Kit and its ligand Stem Cell Factor ( SCF ) are essential for haemopoiesis , melanogenesis and fertility . ^^^ This review focuses on what is known about the regulation of c Kit expression , the functions of SCF and c Kit isoforms , and the nature of the biological responses elicited by this receptor ligand pair with emphasis on the haemopoietic system . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Early signaling pathways activated by c Kit in hematopoietic cells . c Kit is a receptor tyrosine kinase that binds stem cell factor ( SCF ) . ^^^ Studies in white spotting and steel mice have shown that functional SCF and c Kit are critical in the survival and development of stem cells involved in hematopoiesis , pigmentation and reproduction . ^^^ Interaction of SCF with c Kit rapidly induces receptor dimerization and increases in autophosphorylation activity . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The c kit receptor ( CD 117 ) and its ligand stem cell factor ( SCF ) play an important role in the development , differentiation , and survival of normal and malignant hematopoietic cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
BACKGROUND AND OBJECTIVE : Stem cell factor ( SCF ) , and its receptor ( c kit ) play key roles in the expansion and differentiation of hematopoietic progenitor cells , in melanoblasts and primordial germ cells , making it possible that SCF and c kit are involved in neoplastic processes deriving from these cells . ^^^ In this study we investigate how c kit expression is regulated and whether a SCF autocrine loop is essential for survival of sarcoma cell lines . ^^^ RESULTS : In vitro SCF stimulation induces c kit down regulation ; this phenomenon could be connected with receptor internalization , and new protein synthesis is necessary for its re expression . ^^^ INTERPRETATION AND CONCLUSIONS : In sarcoma cell lines , c kit is regulated by differentiation processes ; moreover our results suggest that c kit activity , but probably not the SCF autocrine loop , is essential for survival of these cell lines . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Interestingly , ES cells deficient for Kit , the tyrosine kinase receptor for the stem cell factor ( SCF ) , behaved similarly to wild type ES cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
At this time , approximately 61 % of Rho dull cells and all the Rho bright progenitor and colony forming cells of the engrafted mice were found to be donor derived by c Kit genotyping and by their response to stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Among these factors are signalling molecules , and particularly the stem cell factor ( SCF ) / c kit system . ^^^ The SCF and its receptor c kit are an appropriate example to illustrate the role of signalling molecules in the physiology and pathology of spermatogenesis . ^^^ The SCF / c kit regulates primordial germ cell migration , proliferation and apoptosis during fetal gonadal development . ^^^ The SCF / c kit also regulates spermatogonia proliferation in the adult animal . ^^^ In mutant mice , abnormalities of the SCF / c kit gene expression , such as gene deletion , point mutation , alternative splicing defect , lead to different types of spermatogenesis alterations ( e . g . decrease in primordial germ cell migration , decrease in spermatogonia proliferation ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In order to elucidate the relationship between mast cell character and UP prognosis , we used an immunohistochemical approach to examine the expression of stem cell factor ( SCF ) and c Kit in the skin of patients with UP . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We describe here that lineage phenotype negative ( Lin ) ( ) c kit ( + ) hematopoietic progenitor cells ( HPCs ) from day 13 postcoitus ( dpc ) murine fetal liver ( FL ) can generate dendritic cell ( DC ) precursors when cultured in vitro in the presence of PA 6 stromal cells plus granulocyte / macrophage colony stimulating factor ( GM CSF ) + stem cell factor ( SCF ) + Flt 3 ligand ( Flt3L ) for 12 to 14 days , and develop into mature DCs when stimulated with GM CSF plus mouse tumor necrosis factor alpha ( mTNFalpha ) for an additional 3 to 5 days . ^^^ These NK1 . 1 ( + ) cells could develop into DC precursors at an earlier stage of differentiation by reculturing with PA 6 cells + SCF + Flt3L + GM CSF , but they would be irreversibly committed to NK cell precursors without GM CSF after 3 days , suggesting that GM CSF plays a critical role in controlling the transition of DC and NK cell precursors from 13 dpc FL derived Lin ( ) c kit ( + ) HPCs . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
It was of interest that the aberrant expression of stem cell factor ( SCF ) , a ligand of c kit , was demonstrated on biliary epithelia of the dilated and stenotic bile ducts showing periductal fibrosis and inflammation and also of the proliferated peribiliary glands in hepatolithiasis and PSC , while no such expression was seen in non affected bile ducts in hepatolithiasis or in the bile ducts in normal livers . ^^^ It seems likely that aberrantly expressed SCF on biliary epithelial cells accumulates and stimulates mast cells via the c kit receptor and that these up regulated mast cells induce progressive periductal and portal fibrosis by displaying fibrogenetic factors in hepatolithiasis and PSC . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
A large number of cells with the morphological , phenotypical , and functional characteristics of DCs was generated from both c kit ( low ) and c kit ( low ) populations when cultured with a combination of cytokines ( GM CSF , tumor necrosis factor a [ TNF a ] , interleukin 7 [ IL 7 ] , IL 3 , stem cell factor [ SCF ] , and flt 3 ligand ) ; the cytokine combination studies revealed that SCF and IL 3 in addition to GM CSF and TNF a are essential for DCs to be generated from these primitive populations . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
SCF receptor is encoded by the protooncogene c kit , belongs to the type 3 receptor tyrosine kinase subfamily , and is also expressed on other hematopoietic or non hematopoietic cells . ^^^ Ligation of c kit receptor by SCF induces its dimerization , followed by induction of multiple intracellular signaling pathways leading to cell proliferation and activation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
MC were visualized in terms of metachromasia , reflecting glycosaminoglycan content , and immunohistochemically , visualizing tryptase , chymase and IL 6 as well as the surface markers CD 117 and SCF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We have previously shown that MCF 7 cells ( that naturally express SCF ) transfected with a c kit expression vector exhibit enhanced growth in serum free medium supplemented with IGF 1 . ^^^ Kit , a tyrosine kinase growth factor receptor , and its ligand , stem cell factor ( SCF ) , are commonly coexpressed in breast cancer . ^^^ Consequently , we wished to examine the interaction of Kit / SCF with additional growth factors important in the biology of breast cancer . ^^^ In addition , EGF and heregulin stimulated growth of the ZR 75 1 cell line that naturally coexpresses Kit and SCF was also inhibited by the Kit blocking antibody . ^^^ Taken together these results suggest that coexpression of SCF and Kit may enhance responsiveness to erbB ligands by enhancing activation of the MAPK and PI3K pathways . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We have analyzed the role of the SCF receptor c kit in NK cell differentiation by reconstituting NK deficient mice with fetal liver ( FL ) HSCs of c kit ( / ) ( W / W ) mice . ^^^ In vitro experiments showed that generation of NK cells from FL precursors was reduced in the absence of c kit signaling and that SCF promoted the survival of peripheral c kit ( + ) NK cells . ^^^ We conclude that c kit / SCF interactions in vivo are dispensable for the commitment of HSC to the NK lineage , but they provide essential signals for generating normal numbers of fully mature NK cells . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Murine intraepithelial lymphocytes ( IEL ) express c kit , the receptor for stem cell factor ( SCF ) . ^^^ SCF stimulation of IEL resulted in an expansion of the c kit ( + ) , TCRgammadelta ( + ) cell population . ^^^ SCF induced proliferation was dependent upon SCF c kit interactions , since antibody to c kit blocked this response , and IEL obtained from c kit mutant ( W / W ( 5 ) ) mice failed to respond to SCF . ^^^ SCF treated mice had increased numbers of TCRalphabeta ( + ) and TCRgammadelta ( + ) cell populations , as well as increased numbers of c kit ( + ) and c kit ( ) IEL . ^^^ These data suggest that SCF c kit interactions play an important role in regulating IEL expansion and activation . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
As fibroblasts and mast cells are known to attach via stem cell factor ( SCF ) / c kit interaction when co cultured in monolayers , we also examined the effect of antibodies against SCF and c kit in this system . ^^^ These results indicate that mast cells enhance fibroblast mediated contraction of collagen lattices via direct cell cell contact , mediated in part by SCF / c kit interactions . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Moreover , expression of receptors for IL 7 ( IL 7R ) and stem cell factor ( SCF R , c Kit ) was also induced by CD40L in the majority of BCP ALL cases examined ; however , levels of induction were low compared to those for IL 3R . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Activating mutations in c Kit , the receptor for Stem Cell Factor ( SCF ) , have been identified in dysplasias and leukaemias of the mast cell lineage and have been shown to contribute to transformation in model systems . ^^^ We have found that mutant c Kit ( and normal c Kit in the presence of SCF ) provides a strong differentiation stimulus in normal and immortalised murine early myeloid cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
CD 117 ( SCF receptor ) expression , although very heterogeneous according to the subpopulations and the sources of progenitors evaluated , seems not to correlate with the difference of progenitor cell sensitivity to SCF nor with their proliferative capacity . ^^^ The density of CD 117 antigen expression ( expressed in terms of mean equivalent soluble fluorescence , MESF ) is significantly lower on fresh PB cells than on their BM ( P < 0 . 017 ) and CB ( P < 0 . 004 ) counterparts , particularly in the immature CD34+CD38 population ( 560 + / 131 , 2121 + / 416 and 1192 + / 129 MESF respectively ) ; moreover , when PB and BM CD34+CD38 cells are stimulated for 48 h with SCF + IL 3 , the CD 117 expression decreases by 1 . 5 and 1 . 66 fold , respectively . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The role of the c Kit receptor and its ligand stem cell factor ( SCF ) in the pathogenesis of the disease was analysed in marrow cell clonogenic assays . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The mRNAs of c kit ( SCF receptor ) , bFGF , TNF alpha , mMCP 2 , and mMCP 7 were expressed in both the adrenal glands and the mouse bone marrow derived mast cells ( mBMMCs ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The mRNAs encoding c kit and its ligand stem cell factor ( SCF ) , FSH receptor ( FSH R ) , follistatin , alpha inhibin subunit , and the beta ( A ) and beta ( B ) activin / inhibin subunits were localized in ovaries of ewes with 0 ( ++ ) , 1 ( I+ ) , or 2 ( 2 ) copies of the FecX ( 1 ) gene ( n = 4 9 animals per genotype per gene ) using in situ hybridization . ^^^ Tumors containing granulosa like cells ( n = 3 4 per gene ) expressed the mRNAs encoding alpha inhibin subunit , beta ( A ) , and beta ( B ) activin / inhibin subunits , follistatin , and the FSH R but did not contain detectable amounts of mRNA for c kit or SCF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , the ligand for c Kit , is known to regulate developmental and functional processes of haematopoietic stem cells , mast cells and melanocytes . ^^^ In order to explore the prognostic significance of these molecules in melanoma , total SCF , SCF splice variants and c Kit expression were studied in normal skin melanocytes and in 11 different melanoma cell lines , using reverse transcription polymerase chain reaction , immunocytochemistry and enzyme linked immunosorbent assay . ^^^ Coexpression of both SCF 1 and c Kit was noted in five cell lines , and only one cell line as well as normal melanocytes expressed both SCF 1 and SCF 2 as well as c Kit . ^^^ Of three exemplary melanoma cell lines studied , two expressing SCF mRNA also released SCF spontaneously and on stimulation , whereas the line lacking SCF and c kit mRNA ( SK Mel 23 ) failed to do so . ^^^ These data demonstrate thus that melanoma cell lines , particularly those known to metastasize in vivo , lose the ability to express SCF 2 mRNA , suggesting that this molecule may serve , next to c Kit , as a prognostic marker for malignant melanoma . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) and its receptor KIT play an important role in various biologic phases , such as hematopoiesis , reproduction , and regeneration . ^^^ It has been possible to measure both soluble SCF and soluble KIT using enzyme linked immunosorbent assay since 1993 and 1995 , respectively . ^^^ Although the significance of interaction of soluble SCF with soluble KIT has not yet been elucidated , in certain diseases proteins fluctuate in human sera . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) / c kit plays an important role in the regulation of hematopoiesis , melanogenesis , and spermatogenesis . ^^^ In the testis , the SCF / c kit system is believed to regulate germ cell proliferation , meiosis , and apoptosis . ^^^ However , the signaling pathway for this function of SCF / c kit has not been elucidated . ^^^ Furthermore , AKT , but not protein kinase C zeta , is used by SCF / c kit / PI3 K to activate p70S6K . ^^^ Thus , SCF / c kit uses a rapamycin sensitive PI 3 K / AKT / p70S6K / cyclin D 3 pathway to promote spermatogonial cell proliferation . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
IL 15 and SCF fail to induce NK differentiation and proliferation of CD34+ hematopoietic progenitors from chronic myeloid leukemia patients in contrast to normal stem cells although , both normal and leukemic CD34+ cells display comparable expression of c kit or IL 15 receptor subunits . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In this study , we have examined the expression of c KIT and its ligand , stem cell factor ( SCF ) , in human epithelial ovarian tumors , in normal ovaries and in cultured ovarian surface epithelium ( OSE ) . ^^^ Normal OSE expressed SCF , but not c KIT ; however , epithelial invaginations and inclusion cysts often expressed KIT protein . ^^^ Of 15 benign ovarian tumors and tumors of low malignant potential , 87 % expressed c KIT , and 92 % of these co expressed SCF , suggesting the possibility of autocrine growth regulation . ^^^ Of 35 malignant ovarian cancers , 71 % expressed c KIT ( 92 % co expressed SCF ) , with a trend for decreased c KIT expression in advanced stage disease . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
When BMMC were prepared from W / Wv mice , which lack a functional c kit , or when NIH / 3T3 fibroblasts were substituted with Sl / Sld derived fibroblasts , which lack membrane bound stem cell factor ( SCF ) , a lower , but significant , effect of IL 1 alpha was observed . ^^^ These results indicate that IL 1 alpha stimulates mast cell growth by a fibroblast dependent mechanism , in which SCF / c kit interaction may participate in a major way . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Using various prior observations , we have located potential Kit binding sites on the SCF dimer . ^^^ A superimposition of this dimer onto VEGF in its complex with the receptor Flt 1 places the binding sites on SCF in positions of topographical and electrostatic complementarity with the Kit counterparts of Flt 1 , and a similar model can be made for the complex of PDGF with its receptor . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
SCF mediates its biological effects by binding to and activating a receptor tyrosine kinase designated c kit or SCF receptor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , the c kit receptor ligand , plays a critical role in mast cell ( MC ) development and differentiation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , the ligand for the tyrosine kinase receptor c kit , usually induces differentiation of the CD 34 negative stem cells into CD 34 positive haematopoietic precursors . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
At least 70 % of small cell lung cancers express the Kit receptor tyrosine kinase and its ligand , stem cell factor ( SCF ) . ^^^ Pretreatment of the H 526 small cell lung cancer ( SCLC ) cell line with STI 571 inhibited SCF mediated Kit activation with an IC 50 of 0 . 1 microM as measured by inhibition of receptor tyrosine phosphorylation and 0 . 2 microM as measured by immune complex kinase assay . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
HUVEC dependent MC adhesion and proliferation were inhibited by neutralizing antibodies directed against SCF and vascular cell adhesion molecule ( VCAM ) 1 expressed on HUVECs , and c kit and very late antigen 4 ( VLA 4 ) on MCs . ^^^ The data suggest that two mechanisms ( membrane bound SCF / c kit and VCAM 1 / VLA 4 ) are involved in human MC endothelial cell interactions . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The interaction between stem cell factor ( SCF ) , a ligand produced by Sertoli cells , and its c kit receptor on germ cells is necessary for successful spermatogenesis in animal models . ^^^ SCF can be alternatively spliced into soluble and transmembrane forms , and it is the transmembrane form that is required for spermatogenesis in rodents . c Kit receptors are also present on Leydig cells , and soluble SCF has been implicated in the regulation of testosterone production . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Small cell lung cancer ( SCLC ) is characterized by multiple genetic alterations that include inactivation of the retinoblastoma protein ( Rb ) , the establishment of several autocrine loops including that induced by coexpression of stem cell factor ( SCF ) and Kit , and the ectopic expression and activation of Src family kinases . ^^^ Previous studies have shown that Lck associates with , and becomes activated by , Kit after SCF stimulation of SCLC cells . ^^^ In the present study , we have demonstrated that PP 1 , a pharmacological inhibitor of Src kinases , blocked SCF mediated activation of mitogen activated protein ( MAP ) kinase , but it also inhibited Kit activation . ^^^ Overexpression of Lck reduced the sensitivity of MAP kinase activation to PP 1 without altering the sensitivity of Kit activation , which suggested a role for Lck in SCF mediated MAP kinase activation . ^^^ These data suggest that the SCF / Kit autocrine loop , through activation of Lck and subsequently MAP kinase , and the mutational inactivation of Rb contribute to the loss of G 1 S phase checkpoint regulation during the pathogenesis of SCLC . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Interestingly , a more rapid and marked tyrosine phosphorylation of c kit was also detected in NHMC treated with SCF + ET 1 than NHMC treated with SCF only . ^^^ These data indicate that the synergistic cross talk between SCF and ET 1 signaling is initiated through the pathway of tyrosine phosphorylation of c kit , which results in the enhanced formation of the Shc Grb ( 2 ) complex which leads in turn to the synergistic activation of the Ras / Raf 1 / MEK / MAP kinase loop . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The c kit proto oncogene encodes a transmembrane glycoprotein identical to the receptor for the recently cloned stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In the embryonal gonad the c kit tyrosine kinase receptor and its ligand Stem Cell Factor ( SCF ) are required for the survival and proliferation of primordial germ cells . ^^^ In the postnatal animal , c kit / SCF are required for the production of the mature gametes in response to gonadotropic hormones , i . e . for the survival and / or proliferation of the only proliferating germ cells of the testis , the spermatogonia , and for the growth and maturation of the oocytes . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Reverse transcription polymerase chain reaction ( RT PCR ) analysis to detect gene expression of c kit , the receptor for SCF , was performed using the endometriotic tissue and the eutopic endometrium collected during the operation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The significance of the interaction between Sertoli cell produced stem cell factor ( SCF ) and its receptor , c kit , on Leydig cells ( LCs ) during LC development and differentiation is unknown . ^^^ In the present study , we investigated the potential role of the SCF / c kit system in LC apoptosis and precursor LC proliferation after ethylene dimethane sulfonate ( EDS ) treatment in rats . ^^^ A function blocking anti c kit antibody , ACK 2 , was used to block SCF / c kit interaction at four time points , corresponding to the peak of LC apoptosis and three waves of proliferation of precursor LCs . ^^^ Blockade of SCF / c kit interaction by ACK 2 accelerated LC apoptosis and inhibited proliferation of precursor LCs during the first two waves of precursor LC proliferation around days 3 4 and day 10 , but not the third wave of precursor LC proliferation around day 20 after EDS treatment . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Murine intraepithelial lymphocytes ( IEL ) that express the gamma / delta form of the T cell receptor for antigen ( TCRgammadelta ) also express c kit , the receptor for stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The tyrosine kinase receptor c kit and its ligand , stem cell factor ( SCF ) , are essential for the maintenance of primordial germ cells ( PGCs ) in both sexes . ^^^ In the adult testis , the c kit receptor is re expressed in differentiating spermatogonia , but not in spermatogonial stem cells , whereas SCF is expressed by Sertoli cells under FSH stimulation . ^^^ SCF stimulates DNA synthesis in type A spermatogonia cultured in vitro , and injection of anti c kit antibodies blocks their proliferation in vivo . ^^^ A point mutation in the c kit gene , which impairs SCF mediated activation of phosphatidylinositol 3 kinase , does not cause any significant reduction in PGCs number during embryonic development , nor in spermatogonial stem cell populations . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We have reported that simultaneous activation of glycoprotein ( gp ) 130 and c kit signals by interleukin ( IL ) 6 , soluble IL 6 receptor ( sIL 6R ) , and stem cell factor ( SCF ) promotes proliferation of human hematopoietic progenitor cells and their differentiation into erythroid , myelocytic , and megakaryocytic cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The hemopoietin stem cell factor ( SCF ) and its receptor c kit are expressed in some tumoral cells , including neuroblastoma ( NB ) cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
As described before for the SCF receptor KIT , GM CSFR and NGFR p 75 were partly or entirely downregulated on avidin positive mast cells in scars . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mutant c kit cDNA was transfected into 293T human embryonic kidney cells and the magnitude of autophosphorylation of the mutant KIT was examined with or without the ligand of KIT , stem cell factor ( SCF ) . ^^^ The mutant KIT exhibited constitutive autophosphorylation without SCF stimulation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
SCF and its receptor , Kit , are normally present in both cell surface and soluble forms . ^^^ Both forms of Kit can bind SCF . ^^^ In order to determine if soluble Kit can modulate SCF activity , we produced a fusion protein , Kit Fc , comprised of the extracellular domain of murine Kit and the Fc portion of human IgG ( 1 ) and investigated its ability to bind 125I SCF and to inhibit SCF stimulated hematopoietic colony growth in vitro . ^^^ Scatchard analysis demonstrated that Kit Fc binds iodinated SCF with high affinity ( Kd 570 pM ) . ^^^ Kit Fc also bound to transmembrane SCF displayed on the surface of fibroblasts . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The expression of Kit , the receptor for stem cell factor ( SCF ) , on bovine peripheral blood cells ( PBCs ) was examined by using monoclonal antibodies against the bovine Kit protein . ^^^ These results indicate that Kit is expressed on subsets of lymphocytes in bovine peripheral blood , but the ligand of Kit , SCF , does not directly induce the proliferation of this cell population . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We analyzed the expression of c kit and its ligand stem cell factor ( SCF ) in a panel of six Ewing ' s sarcoma cell lines . ^^^ All cell lines exhibited substantial levels of surface c kit expression , and five of six displayed transmembrane SCF on the cell surface . ^^^ Expression of c kit was down modulated in all lines by exposure to exogenous SCF . ^^^ This could be dependent on the down modulation of c kit expression observed in SCF pretreated cells , leading to a reduced sensitivity to the chemotactic and proliferative actions of SCF . ^^^ Our results indicate that the response to SCF mediated by c kit may be involved in growth , migration , and metastatic ability of Ewing ' s sarcoma cells . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In the bone marrow , B lineage cells were proportionately increased , reflecting enhanced production of pro B cells that resulted in part from hypersensitivity of precursors to SCF , the ligand for c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
OBJECTIVE : To characterize the expression and distribution of the Kit receptor in bovine bone marrow cells ( BMC ) and to define the function of its ligand , stem cell factor ( SCF ) . ^^^ Our results suggest that the Kit receptor and its ligand , SCF , are involved in early stages of granulopoiesis in calves . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The most common factor that stimulates mast cell growth , migration and differentiation is stem cell factor ( SCF ) , and the interaction of SCF and its receptor , KIT ( tyrosine kinase transmembrane receptor ) , appears to be the key event in the recruitment and proliferation of mast cells . ^^^ OBJECTIVES : To determine whether any altered metabolism of SCF and / or KIT is present in patients with AD . ^^^ METHODS : We measured serum levels of soluble SCF ( sSCF ) and soluble KIT ( sKIT ) using enzyme linked immunosorbent assay in 54 patients with AD , five patients with erythrodermic psoriasis vulgaris and 64 healthy individuals . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Maintenance of LTR HSCs is mediated by a mechanism other than SCF signaling through its receptor ( c Kit ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Because stem cell factor ( SCF ) , the ligand for Kit ( CD 117 ( + ) ) , is a chemoattractant for CD 117 ( + ) cells and one feature of mastocytosis is an abnormal collection of mast cells in tissues derived from CD 34 ( + ) CD 117 ( + ) mast cell precursors , the hypothesis was considered that the D816V mutation would enhance chemotaxis of these precursor cells . ^^^ Constructs encoding wild type Kit or Kit bearing the D816V mutation were transfected into Jurkat cells , labeled with Calcein AM , and migration to SCF assessed in the presence or absence of tyrosine kinase inhibitors . ^^^ Chemotaxis to SCF was enhanced in D816V transfectants compared to wild type Kit transfectants ( P < . 002 ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Their effects on stem cell factor ( SCF ) dependent Kit autophosphorylation and SCLC cell growth were also examined . ^^^ Inhibition of SCF stimulated Kit activation and cell growth in the H 526 cell line was dose dependent . ^^^ At concentrations that inhibited SCF stimulated H 526 cell growth , there was little effect on insulin like growth factor 1 stimulated growth , suggesting that these compounds exhibit reasonable selectivity for inhibition of Kit mediated proliferation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
This information in turn led to the critical observation that a substantial number of patients with mastocytosis exhibit activating mutations in c kit , the receptor for SCF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In vitro addition of stem cell factor ( SCF ) to c kit expressing A ( 1 ) A ( 4 ) spermatogonia from prepuberal mice stimulates their progression into the mitotic cell cycle and significantly reduces apoptosis in these cells . ^^^ All the events associated with SCF induced cell cycle progression are inhibited by the addition of either a PI3K inhibitor or a mitogen activated protein kinase kinase ( MEK ) inhibitor , indicating that both MEK and PI3K are essential for c kit mediated proliferative response . ^^^ Thus , SCF effects on mitogenesis and survival in c kit expressing spermatogonia rely on different signal transduction pathways . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) binds the receptor tyrosine kinase c Kit and is critical for normal hematopoiesis . ^^^ Immortalized murine progenitor cells ( MIHCs ) transduced with wild type c Kit proliferate in response to SCF , whereas cells expressing D816V c Kit ( MIHC D816V ) are factor independent and tumorigenic . ^^^ SCF stimulates association of p85PI3K with phosphorylated tyrosine 721 of wild type c Kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
They develop from bone marrow derived progenitor cells , primed with stem cell factor ( SCF ) , which mediates its actions by interacting with the SCF receptor or c kit on the cell surface . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
All NCCmelan 5 cells were positive for KIT ( SCF receptor ) , HMB 45 ( human melanosomal antigen ) , tyrosinase related protein 1 ( TYRP 1 ) , tyrosinase related protein 2 ( TYRP 2 ) , tyrosinase and endothelin receptor B ( EDNRB ) and all could oxidize 3 , 4 dihydroxyphenylalanine ( DOPA ) to form melanin . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) initiates its biological effects by binding to its receptor Kit . ^^^ Structure function analysis of the extracellular region of Kit has implicated the first three immunoglobulin like domains in SCF binding , and the fourth immunoglobulin like domain in receptor dimerization . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To this end , we compared expression of RNA encoding the receptors for interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , IL 6 , leukemia inhibitory factor ( LIF ) and stem cell factor ( SCF ) in populations of primitive hematopoietic progenitors ( lineage marker negative , Lin ( ) , and Lin ( ) c Kit ( + ) ) by RT PCR and in situ RT PCR . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Recently , inhibition of stem cell factor ( SCF ) induced c kit phosphorylation and cell proliferation by STI 571 was reported in the human myeloid cell line MO7e . ^^^ SCF induced phosphorylation of c kit in MO7e cells was inhibited by STI 571 . ^^^ In a c kit positive AML 4 cell line , as well as in AML samples , c kit phosphorylation was not induced by SCF exposure , suggesting that in these cases , the receptor could not be functionally activated . ^^^ In conclusion , with the exception of MO7e , SCF did not induce phosphorylation of c kit , and cell proliferation was not modulated in the presence of STI 571 . ^^^ We did not detect any SCF independent c kit phosphorylation in our experimental systems . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Review : melanocyte migration and survival controlled by SCF / c kit expression . ^^^ Finally , SCF transgene expression demonstrated that part of the c kit dependent step is regulated by spatiotemporally specific ligand expression and also indicated the presence of c kit independent melanocyte stem cells in postnatal skin . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
As responsible molecules for stromal cell dependent hematopoiesis , expression of stem cell factor ( SCF ) ( a ligand of c Kit ) , vascular cell adhesion molecule ( VCAM 1 ) ( a ligand of VLA 4 ) , and secretion of interleukin ( IL ) 6 were increased by OSM . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Interleukin 3 ( IL 3 ) and erythropoietin ( Epo ) , with or without stem cell factor ( SCF ) , upregulated c kit protein and its mRNA expression . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
These results suggest that IL 6 family cytokines stimulate mast cell growth by a fibroblast dependent mechanism , and also suggest the existence of another pathway between BMMC and NIH / 3T3 fibroblasts cooperating with the SCF / c kit pathway . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Interestingly , these cells were less responsive to stem cell factor ( SCF ) despite c kit expression on hematopoietic stem cells ( HSCs ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Previous studies have demonstrated that activation of Kit by stem cell factor ( SCF ) , its natural ligand , or by gain of function point mutation in its intracellular domain , confers significant protection against apoptosis induced by growth factor deprivation or radiation . ^^^ This study shows that daunorubicin induced apoptosis and cytotoxicity were reduced in the murine Ba / F3 cells transfected with Kit ( Ba / F3 Kit ) in the presence of SCF and in Ba / F3 cells transfected with a constitutively active Kit variant ( Ba / F3 KitDelta 27 ) , compared to either parental Ba / F3 ( Ba / F3 wt ) or unstimulated Ba / F3 Kit cells . ^^^ In Ba / F3 wt and in Ba / F3 Kit cells , daunorubicin stimulated within 8 15 min neutral sphingomyelinase and ceramide production but not in SCF stimulated Ba / F3 Kit or in Ba / F3 KitDelta 27 whereas all Ba / F3 cells were equally sensitive to exogenous cell permeant C 6 ceramide . ^^^ In Ba / F3 Kit , SCF induced Kit activation resulted in a rapid phospholipase Cgamma ( PLCgamma ) tyrosine phosphorylation followed by diacylglycerol release and protein kinase C ( PKC ) stimulation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The growth and differentiation of human mast cells are quite dependent on stem cell factor ( SCF ) , the ligand for the protein products of c kit . ^^^ Recent studies have demonstrated that all adult patients examined so far carry c kit point mutations , leading to SCF independent autophosphorylation of the receptor and autonomous cell growth . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
At each stage , key events of oocyte development and differentiation , such as the c kit reexpression , the early zona pellucida assembly , and the beginning of follicologenesis , were observed to occur independently by the presence of SCF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The signals generated by the binding of stem cell factor ( SCF ) to c kit receptor tyrosine kinase ( KIT ) are essential for their development and survival . ^^^ A double gene dose of mutant alleles at either the SCF or KIT locus results in a decrease of mast cells . ^^^ Although the phenotype of the few mast cells remaining in SCF and KIT mutant mice appeared to be normal , the phenotype of mast cells was abnormal in MITF mutant mice . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Although the initial events leading to mastocytosis have not yet been unraveled , acquired alterations in the c kit gene coding for the receptor of stem cell factor ( SCF ) , a major cytokine involved in MC growth , have been described in a significant number of patients . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) has crucial roles in proliferation , survival , and differentiation of hematopoietic stem cells and mast cells through binding to c Kit receptor ( KIT ) . ^^^ However , little is known about the intracellular signaling pathway of SCF / KIT mediated cell migration . ^^^ On stimulation with SCF , BAF 3 expressing KIT ( WT ) ( WT ) showed cell migration and Ca ( 2+ ) mobilization . ^^^ In Y567F , Lyn activation on SCF stimulation decreased and C terminal Src kinase ( Csk ) suppressed KIT mediated Ca ( 2+ ) influx and cell migration , suggesting that Y 567 mediated Src family kinase ( SFK ) activation leads to Ca ( 2+ ) influx and migration . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Disruption of the c Kit / stem cell factor ( SCF ) signaling pathway interferes with the survival , migration , and differentiation of melanocytes during generation of the hair follicle pigmentary unit . ^^^ We examined c Kit , SCF , and S 100 ( a marker for precursor melanocytic cells ) expression , as well as melanoblast / melanocyte ultrastructure , in perinatal C57BL / 6 mouse skin . ^^^ These findings suggest that melanoblasts express c Kit as a prerequisite for migration into the SCF supplying hair follicle epithelium . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Fluorescent activated cell sorting ( FACS ) analysis was performed with the M 86 monoclonal antibody ( specific for Gal ) , lineage markers , and biotinylated stem cell factor ( SCF ) to detect c kit expression . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Kit / stem cell factor ( SCF ) has been reported to be involved in survival and proliferation of male differentiating spermatogonial cells . ^^^ This kinetics study was designed to assess the role of Kit / SCF during spermatogenesis in mice , and the extent of male programmed germ cell death was measured between 8 and 150 days of age . ^^^ These results suggest involvement of Kit / SCF at different stages of spermatogenesis . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
BCR ABL mediated an increase in c kit expression that correlated with an enhanced mitogenic response to SCF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Vesicle formation upon SCF induced dimerization of the receptor was strictly dependent on kinase activity of c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
SU 5614 induced growth arrest and apoptosis in c kit expressing Kasumi 1 , UT 7 , and M 07e cells and inhibited the stem cell factor ( SCF ) induced tyrosine phosphorylation of c kit . ^^^ The sensitivity of Kasumi 1 cells towards the growth inhibitory activity of SU 5614 was caused by an autocrine production of SCF , but not by transforming mutations of c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The induction of LTP was prevented by perfusion with rmSCF started 30 min before tetanus , while preincubation of slices with antibody for SCF receptor , c kit , blocked LTP , suggesting that the intrinsic SCF is involved in the induction of LTP and the blockade of LTP by rmSCF might be due to an occlusion of SCF / c kit signaling . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) is essential to the migration and differentiation of melanocytes during embryogenesis because mutations in either the SCF gene , or its ligand , KIT , result in defects in coat pigmentation in mice . ^^^ Using a neural crest cell ( NCC ) primary culture system from wild type mice , we previously demonstrated that KIT positive and / or L 3 , 4 dihydroxyphenylalanine ( DOPA ) positive melanocyte precursors proliferate following the addition of SCF to the culture medium . ^^^ More KIT positive cells and DOPA positive cells were detected in the presence of SCF on ECM coated wells than on non coated wells . ^^^ In contrast , in the absence of SCF , few DOPA positive cells and KIT positive cells were detected on either the ECM coated or non coated wells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Most of the cells , cultured in the presence of SCF and IL 6 for 10 weeks , expressed c kit and contained a significant amount of histamine and tryptase and a low amount of chymase . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Key regulators of differentiation of MCs appear to be stem cell factor ( SCF ) and its tyrosine kinase receptor KIT ( c kit proto oncogene product=CD117 ) , downstream acting elements , and the mi transcription factor ( MITF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The SCF receptor , c kit , was expressed in neuronal cultures and in neuroproliferative zones of the adult rat brain , and in vivo administration of SCF increased BrdU labeling of immature neurons in these regions . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The stem cell factor c kit signaling pathway ( SCF / c kit ) has been previously implicated in normal hematopoiesis , melanogenesis , and gametogenesis through the formation and migration of c kit ( + ) cells . ^^^ Here we show that the activation of c kit by SCF specifically induces the expression of Slug , a Snail family member . ^^^ Kit ( + ) cells derived from Slug mutant mice exhibit migratory defects similar to those of c kit ( + ) cells derived from SCF and c kit mutant mice . ^^^ Endogenous Slug is expressed in migratory c kit ( + ) cells purified from control mice but is not present in c kit ( + ) cells derived from SCF mutant mice or in bone marrow cells from W / W ( 5 ) mice , though Slug is present in spleen c kit ( + ) cells of W / W ( 5 ) ( mutants expressing c kit with reduced surface expression and activity ) . ^^^ SCF induced migration was affected in primary c kit ( + ) cells purified from Slug / mice , providing evidence for a role of Slug in the acquisition of c kit ( + ) cells with ability to migrate . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In this study , we have compared the consequence ( s ) of activating Kit , the receptor for SCF by MA SCF with S SCF , and an obligate membrane restricted ( MR ) form of SCF ( MR SCF ) on erythroid cell survival , proliferation , cell cycle progression , and the activation of p 38 and ERK MAP kinase pathways . ^^^ Activation of Kit by MR SCF was associated with a significantly lower incidence of apoptosis and cell death in erythroid cells compared to either other isoform . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Results show that , in both Ba / F3 and 32D murine cell lines transfected with wild type c kit , stem cell factor ( SCF ) stimulation resulted in a significant reduction of IR induced apoptosis and cytotoxicity , whereas DNA repair remained unaffected . ^^^ The SCF protective effect was maintained in 32D KitYF 719 cells in which the PI3K / Akt signaling pathway is abolished due to mutation in Kit docking site for PI3K . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Although some in vivo experiments showed that the c kit receptor tyrosine kinase ( KIT ) and its ligand , stem cell factor ( SCF ) , might be required for the development of murine ICCs near birth , in vitro experiments would be useful to clarify the role of SCF KIT system for the development of ICCs . ^^^ Murine gastrointestinal cells from embryos or neonates were cultured with SCF and stained with anti KIT antibody and / or alcian blue . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In particular , we analysed the expression of c kit and its ligand stem cell factor ( SCF ) in a panel of HD derived cell lines and fibroblasts from HD involved lymph nodes ( HDF ) . ^^^ While c kit was expressed by HD derived cell lines , usually in the absence of SCF , this latter molecule , in its soluble and / or membrane bound ( mb ) form , was in turn expressed at a high level by primary HDF . ^^^ In vitro adhesion between HD derived cell lines and HDF was mainly mediated by c kit / SCF interactions , and this phenomenon was significantly inhibited by an excess of soluble SCF or by neutralizing anti c kit monoclonal antibodies . ^^^ Taken together , our data indicated the expression of functional c kit receptor by H RS cells and suggests a role of SCF in the pathobiology of cHD . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In mice , mutations in the c kit gene and in the gene encoding stem cell factor ( SCF ) cause pleiotropic defects among which sterility . ^^^ A possible involvement of the SCF / c kit system in human spermatogenesis was investigated . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In this study , we stained the stem cell factor ( SCF ) and the Kit receptor in tryptase positive mast cells , since these molecules are essential for mast cell survival , growth , migration and activation . ^^^ In the psoriatic skin of ten patients , lesional specimens showed significantly higher numbers of SCF positive dermal cells as well as a higher percentage ( 88+ / 12 % vs 46+ / 26 % , P=0 . 004 ) of Kit positive mast cells than nonlesional skin . ^^^ In conclusion , our findings show that the expression of SCF increases rapidly in the early stages of wound healing but declines thereafter , whereas the expression of Kit in mast cells is induced slowly in healing wounds . ^^^ In chronic wounds as well as in psoriatic lesions , both SCF and Kit are intensely expressed . ^^^ Thus , it seems possible that SCF and Kit receptor interact , and this could lead to persistent mast cell activation and growth in chronic wounds and psoriasis , whereas only temporary mast cell activation is apparently needed in healing wounds . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Consistent with the in vivo repopulating deficiency , when Sca 1 ( + ) c kit ( + ) lin ( ) ( KLS ) cells were isolated and stimulated with growth factors in vitro , up to a 13 fold reduced expansion of total nucleated cells was observed in response to cocktails containing interleukin 3 ( IL 3 ) , IL 6 , stem cell factor ( SCF ) , Flt 3 ligand , and thrombopoietin . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To further investigate the hemoglobin modulating effects of SCF , we examined the surface expression pattern of the SCF receptor , CD 117 , among maturing erythroblasts . ^^^ SCF related increases in the HbF / HbA ratio correlated with the expression pattern of CD 117 . ^^^ These results suggest that the HbF response to SCF is greatest at the late progenitor stage as a function of surface CD 117 expression . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
PIR B and c kit ( or mast / SCF receptor ) coligation also inhibited SCF induced mast cell responses . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) and its receptor c kit take part in the regulation of developmental processes of mast cells , hematopoietic stem cells , and melanocytes , as well as in the growth control of human malignancies . ^^^ To explore the possible role of the SCF c kit system and of mast cells in pancreatic cancer , the concomitant expression and distribution of the two molecules were examined in 17 normal and 26 cancerous human pancreatic tissues and in 6 cultured pancreatic cancer cell lines . ^^^ In addition , the effects of SCF and of the c kit tyrosine kinase inhibitor STI 571 on the growth of the cancer cell lines and of the normal pancreatic ductal cell line TAKA 1 were assessed . ^^^ Mast cells , identified by tryptase and chymase immunostaining on consecutive tissue sections , showed immunoreactivity for SCF and c kit in both normal and cancerous specimens and their number was significantly increased ( p = 0 . 03 ) in pancreatic cancer compared with the normal pancreas . ^^^ In conclusion , the SCF c kit system , possibly with the contribution of mast cells , may have a growth regulating role in the normal pancreas , which is altered during malignant transformation . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Although a role for stem cell factor ( SCF ) and SCF receptor ( KIT ) in long term survival of MCs has been proposed , the underlying biochemical mechanisms remain unknown . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Initial data demonstrated that eosinophils have significant levels of surface c kit protein , SCF receptor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The cytokine stem cell factor ( SCF ) and its interaction with its receptor c kit plays an important role in the development of germ cells in eutherians . ^^^ To investigate the putative roles of the SCF / c kit system in marsupials , recombinant Australian brushtail possum ( Trichosurus vulpecula ) SCF was purified after secretion by the methylotrophic yeast Pichia pastoris . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The Src selective kinase inhibitor PP 1 also inhibits Kit and Bcr Abl tyrosine kinases . 4 amino 5 ( 4 methylphenyl ) 7 ( t butyl ) pyrazolo [ 3 , 4 d ] pyrimidine ( PP 1 ) was identified as an Src selective tyrosine kinase inhibitor and has been used extensively to investigate signaling pathways involving Src kinases , including events downstream of the stem cell factor ( SCF ) receptor c Kit . ^^^ PP 1 inhibited SCF induced c Kit autophosphorylation in intact cells and blocked the activation of mitogen activated protein kinase and Akt . ^^^ SCF induced c Kit phosphorylation was also inhibited by the related inhibitor 4 amino 5 ( 4 chlorophenyl ) 7 ( t butyl ) pyrazolo [ 3 , 4 d ] pyrimidine ( PP 2 ) and by STI 571 but not by the Src inhibitor SU 6656 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) and its receptor c Kit have been identified as important regulators of SCLC viability and are coexpressed in approximately 40 70 % of SCLC specimens . ^^^ SCF and SDF 1alpha increased cellular proliferation over a course of 72 h in both the c Kit and the CXCR 4 positive NCI H 69 SCLC cell line . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Kit was constitutively phosphorylated on tyrosine residues and associated with phosphatidylinositol 3 ' kinase ( PI 3 kinase ) in both variants of HMC 1 , but this did not lead to a constitutive phosphorylation of Akt or extracellular regulated protein kinase ( ERK ) , which are signalling molecules normally activated by the interaction of stem cell factor ( SCF ) with Kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
These findings were positively correlated with the up regulation of cytokine mRNA expression such as granulocyte colony stimulating factor ( GM CSF ) , erythropoietin ( EPO ) , thrombopoietin ( TPO ) , stem cell factor ( SCF ) , and c Kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The c kit proto oncogene plays a dual role in the control of male fertility in mice through two alternative gene products : ( 1 ) . c kit [ the transmembrane tyrosine kinase receptor for stem cell factor ( SCF ) ] , which is expressed and functional in differentiating spermatogonia of the postnatal testis , in which c kit is essential for pre meiotic proliferation ; and ( 2 ) . tr kit , an intracellular protein which is specifically accumulated during spermiogenesis through the use of an alternative intronic promoter , and which is able to trigger mouse egg activation when microinjected into the cytoplasm of metaphase 2 arrested oocytes . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Donor cells were derived from the complementary mutant strain W / W ( 5 ) , which also exhibit anemia , due to mutations in c kit , the SCF receptor expressed on hematopoietic stem cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Neuronal stimulation was induced in rats by systemic administration of kainic acid ( KA ) to determine if such stimulation is responsible for changes in the expression patterns of c Kit and stem cell factor ( SCF ) in cerebellar synapses between inhibitory interneurons and Purkinje cells . ^^^ Using immunocytochemistry and immunoblotting analyses , we demonstrate that c Kit receptor tyrosine kinase and its ligand SCF are present on the pre and postsynaptic sides of inhibitory synapses on Purkinje cells . ^^^ Expression of both c Kit and SCF are significantly elevated in the Purkinje cell layer 24 hr after KA administration , and the Purkinje cell layer exhibits a loss of calbindin D 28K immunoreactivity . ^^^ Also during the first 48 hr after KA treatment , SCF levels increase in axonal processes of Purkinje cells , and these SCF positive axons correlate with c Kit positive pinceau structures . ^^^ The increased expression of c Kit and SCF in response to KA induced neuronal stimulation may indicate that c Kit receptor tyrosine kinase and its ligand SCF function in the inhibitory synapse between cerebellar interneurons and Purkinje cells , and that this role is most pronounced during the first 48 hr after KA treatment . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
It was concluded that cord blood CD 34 ( + ) stem / progenitor cells are capable of expressing Flt 3 and c kit for as long as 2 3 weeks in liquid medium , during the first week of culture , SCF and FL enhanced the generation of cells and progenitors notably . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The expression of the SCF receptor ( c kit ) was also detected in the Mo7e cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Recently , it could be shown that systemic mastocytosis ( SM ) is a clonal disorder often exhibiting mutations of c kit , a protooncogene encoding the tyrosine kinase receptor for stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Here , we show that activation of c Kit by its ligand , stem cell factor ( SCF ) , cooperates with alpha 4 integrin in inducing directed migration of mast cells on fibronectin . ^^^ Consistent with the pharmacologic results , abrogating the binding of the p85alpha subunit of class IA PI 3kinase to c Kit also resulted in inhibition of SCF induced migration on fibronectin . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
One such drug is imatinib mesylate ( imatinib , Glivic / Gleevec ) , an orally administered 2 phenylaminopyrimidine derivative that is a competitive inhibitor of the tyrosine kinases associated with platelet derived growth factor ( PDGF ) receptors , the Abelson ( ABL ) protein and the KIT protein ( also known as stem cell factor [ SCF ] receptor ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Imatinib mesilate ( Glivec ) is a protein tyrosine kinase inhibitor that potently inhibits the Bcr Abl tyrosine kinase as well as the receptors for platelet derived growth factor ( PDGF ) and stem cell factor ( SCF ) , c Kit , at in vitro and cellular kinase assay levels . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) supports proliferation and survival of early hematopoietic cells by binding to the c kit receptor . ^^^ The levels of drug induced caspase processing are significantly reduced in erythroblasts treated with SCF , indicating that activation of the c kit receptor generates antiapoptotic signals acting before amplification of the caspase cascade . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mutant D816VKit and wild type Kit were expressed in murine primary haemopoietic cells and grown in stem cell factor ( SCF ) or the absence of factors . ^^^ Expression of wild type Kit in the presence of SCF also failed to cause transformation and induced differentiation of mast cells and megakaryocytes . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
METHODS : In this study , renal biopsy specimens from 28 patients with a histopathologic diagnosis of CGN were evaluated immunohistochemically for the presence of mast cells , SCF , and its receptor ( c kit ) . ^^^ CONCLUSION : These results show the potential involvement of mast cells and their growth factor SCF / c kit in CGN . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
STUDY DESIGN AND METHODS : CD34+ cells were analyzed for the expression of CD 38 , CD 133 , and CD 117 and cultured in serum free culture medium with four cytokine combinations : SCF plus thrombopoietin plus flt 3 ligand ( STF ) , STF plus IL 3 , STF plus IL 6 , and STF plus IL 6 plus IL 3 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The striking difference in flt 3 and c kit expression on mouse and candidate human HSCs translated into a corresponding difference in flt 3 and c kit function because FL was more efficient than SCF at supporting the survival of candidate human HSCs . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Kit and its ligand stem cell factor ( SCF ) play a fundamental role in hematopoiesis , melanogenesis and gametogenesis . ^^^ Further , in vitro treatment with SCF was shown to downregulate Fas on fibroblasts expressing exogenous Kit through activation of PI 3 kinase / Akt . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Imatinib has also been shown to be the only effective drug therapy in the treatment of patients with metastatic gastrointestinal stromal tumors expressing the stem cell factor ( SCF ) receptor Kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Coexpression for c Kit receptor and its ligand stem cell factor ( SCF ) has been described in neuroblastoma ( NB ) cell lines and tumors , suggesting the existence of an autocrine loop modulating tumor growth . ^^^ We evaluated c Kit and SCF expression by immunohistochemistry in a series of 75 primary newly diagnosed neuroblastic tumors . ^^^ Immunostaining for c Kit was found in 10 / 75 and for SCF in 17 / 75 , with 5 / 10 c Kit positive tumors also expressing SCF . ^^^ For both , c Kit and SCF staining were predominantly found in the most aggressive subset of tumors , i . e . , those amplified for MYCN : c Kit was detected in 8 / 14 amplified vs . 2 / 61 single copy ( p < 0 . 001 ) , and SCF in 9 / 14 amplified vs . 8 / 61 single copy tumors ( p < 0 . 001 ) . ^^^ Furthermore , the association of c Kit expression with advanced stage ( 3 or 4 ) ( p=0 . 001 ) and of SCF expression with adrenal primary ( p=0 . 03 ) was substantiated . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , a key regulator of hematopoiesis signaling through c kit , enhanced the proliferation of TMBL 1 cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Upon binding its ligand , stem cell factor ( SCF ) , c Kit forms an active dimer that autophosphorylates itself and activates a signaling cascade that induces cell growth . ^^^ Disease causing human mutations that activate SCF independent constitutive expression of c Kit are found in acute myelogenous leukemia , human mast cell disease , and gastrointestinal stromal tumors . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The stem cell factor c kit signaling pathway ( SCF / c kit ) has been previously implicated in the hematopoietic recovery which prevents death from lethal irradiation , but the molecular mechanisms that mediate this biological effect are unknown . ^^^ Since mutations on SCF , c kit and Slug genes have a similar phenotype in mice , we examined if Slug could complement the radiosensitivity of kit deficient mice . ^^^ This effect can not be recovered by activating SCF / c kit in lethally irradiated Slug deficient mice , as SCF treated mice did not demonstrate stimulation of hematopoietic recovery leading to survival of the Slug deficient mice . ^^^ We conclude that the zinc finger transcription factor Slug is absolutely necessary for survival from lethal irradiation and identify Slug as the molecular target that mediates the radioprotection through SCF / c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Several signaling pathways have been recognized in normal c kit mediated signal transduction following stem cell factor ( SCF ) stimulation including Janus kinase ( JAK ) / signal transducer and activator of transcription ( STAT ) , mitogen activated protein kinase ( MAPK ) and phosphoinositol 3 kinase ( PI 3 K ) pathways . ^^^ However , the signal transduction cascades specific for the SCF independent c kit activation in GIST remains to be elucidated . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
This concept represented the rationale for the initiation of a multicenter phase 2 trial of SU 5416 , a small molecule inhibitor of phosphorylation of VEGF receptors 1 and 2 , c kit , the SCF receptor , and fms like tyrosine kinase 3 ( FLT 3 ) in patients with advanced AML . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Although activating c kit mutation Asp 816 to Val was not detected by PCR , the presence of KIT protein ( CD 117 ) positive intratumoral myofibroblastic spindle cells in chorioangiomas and chorangiomatosis might suggest involvement of the stem cell factor ( SCF ) receptor in pathologically enhanced angiogenesis . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , the ligand of the c kit protooncogene product , is a major regulator and chemoattractant of mast cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
BACKGROUND : Ewing ' s sarcoma cells express c kit , a receptor tyrosine kinase , and its ligand , stem cell factor ( SCF ) , creating a potential autocrine loop that may promote tumor survival . ^^^ Imatinib inhibited SCF mediated c kit phosphorylation ( IC 50 = 0 . 1 0 . 5 microM ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Previous studies have demonstrated that imatinib mesylate also inhibits the survival and functions of normal mast cells by interfering with the receptor tyrosine kinase for stem cell factor ( SCF ) , c kit , which is expressed by mast cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mutations in the dominant white spotting ( W ; c kit ) and stem cell factor ( Sl ; SCF ) genes , which encode the transmembrane tyrosine kinase receptor and its ligand , respectively , affect both the proliferation and differentiation of many types of stem cells . ^^^ To characterize spermatogenesis in c kit / SCF mutants and to understand the role of c kit signal transduction in spermatogonial stem cells , the existence , proliferation , and differentiation of spermatogonia were examined in the W / Wv mutant mouse testis . ^^^ The c kit / SCF signal transduction system appears to be necessary for the maintenance and proliferation of differentiated c kit receptor positive spermatogonia but not for the initial step of spermatogonial cell differentiation . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
BACKGROUND : The expression of c kit and / or its ligand , stem cell factor ( SCF ) , has been related to tumor proliferation , in several tumor systems . ^^^ MATERIALS AND METHODS : We analyzed the expression of the SCF / its receptor ( c kit ) mRNA and the production of soluble SCF in a human malignant fibrous histiocytoma ( MFH ) cell line ( TNMY 1 ) . ^^^ RESULTS : Immunocytochemical analysis revealed that the TNMY 1 cells were positive for both SCF and c kit . ^^^ Reverse transcriptase polymerase chain reaction ( RT PCR ) showed that the TNMY 1 cell line expressed mRNA for SCF and c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
OBJECTIVE : STI 571 is a tyrosine kinase inhibitor which inhibits the kinase activity of kit , the receptor for stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) / c kit system is critical for human mast cell development . ^^^ STI 571 at concentrations of 10 ( 6 ) M or higher almost completely abolished the SCF dependent progeny generation from cord blood derived cultured mast cells through an inhibition of the tyrosine phosphorylation of c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Follicular expression of c Kit / SCF and inhibin alpha in mouse ovary during development . ^^^ We performed an immunohistochemical ( IHC ) study to determine the follicular expressions of c kit , SCF , and inhibin alpha at different developmental stages in mouse ovary . ^^^ IHC for c kit , SCF , and inhibin alpha was carried out . c Kit and SCF were expressed on oogonia regardless of the developmental stage . ^^^ Immunoreactive c kit and SCF antigens were expressed on oocytes of primordial and primary follicles of neonate mouse ovaries . ^^^ In 21 dpp mouse ovary , the expression of c kit / SCF in oocytes gradually decreased as the follicles developed . c Kit / SCF was expressed strongly in oocytes of preantral follicles and weakly in granulosa and thecal cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
A soluble CAR SCF fusion protein improves adenoviral vector mediated gene transfer to c Kit positive hematopoietic cells . ^^^ The ability of CAR SCF to enhance Ad 5 eGFP infectivity was highly dependent on cellular c Kit expression levels . ^^^ CONCLUSIONS : The CAR SCF fusion protein assists Ad 5 mediated transduction to c Kit ( + ) CAR ( ) hematopoietic cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Point mutation in the SH 2 domain of Lnk , which abolishes the binding capability of Lnk to c Kit upon stimulation with stem cell factor ( SCF ) , led to loss of Lnk dependent inhibition of hematopoietic cell development in AGM cultures , suggesting Lnk mediated inhibition of the SCF / c Kit signaling pathway . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Therefore , STI 571 was examined for therapeutic efficacy against malignant Gastro Intestinal Stromal Tumors ( GIST ) , which are mainly caused by aberrant expression of a mutated c Kit that is constitutively active without binding of a ligand , stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In addition , populations of HSC were isolated using non neutralizing and neutralizing antibodies to the SCF receptor c kit , and their spatial distribution was analyzed post transplant . ^^^ The role of tm SCF was confirmed by analyzing the spatial distribution of HSC isolated using a neutralizing antibody to c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
BACKGROUND : Little information is available on the expression of stem cell factor ( SCF ) and its receptor c kit in soft tissue tumors of a fibrohistiocytic origin , including malignant fibrous histiocytoma ( MFH ) . ^^^ MATERIALS AND METHODS : We investigated the endogenous expression of SCF and c kit in 43 MFH tissue samples using immunohistochemical techniques . ^^^ CONCLUSION : Our data indicate that any direct autocrine effects of the SCF / c kit system on cell growth regulation are precluded in most MFH cases studied , but it is speculated that SCF might indirectly influence tumor growth by promoting local neovascularization . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
And 6 / 13 cell lines and 27 / 44 primary AML samples exhibit co expression of the KIT receptor and ligand ( SCF ) while 10 / 13 cell lines and 35 / 44 primary AML samples exhibit co expression of the FMS receptor and ligand ( CSF 1 ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In transient transfection assays , mutant isoforms D816V , D816H , Y823D , and N822K were constitutively phosphorylated in the absence of the natural ligand for KIT , stem cell factor ( SCF ) . ^^^ In contrast , activation of T801I and wild type KIT required SCF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Ectopic expression of SOCS 6 in Ba / F3 KIT cell line decreased cell proliferation in response to SCF but not SCF induced chemotaxis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In mammalian skin , stem cell factor ( SCF ) regulates the proliferation and maturation of mast cells and melanocytes , which are thought to be the only cutaneous cells that express the Kit tyrosine kinase receptor ( Kit ) and respond to epithelial and mesenchymal derived SCF . ^^^ Expression of Kit and SCF was strongly hair cycle dependent , suggesting a functional role of epithelial Kit expression in hair growth control . ^^^ This was supported by the observation that mice unable to respond to SCF stimulation ( Kit ( W ) / Kit ( W 5 ) ) showed a significant retardation of anagen development compared to their wild type littermates . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Neuroblastoma cells express platelet derived growth factor ( PDGF ) , stem cell factor ( SCF ) , and vascular endothelial growth factor ( VEGF ) and their respective receptors , PDGFR , c Kit , and Flk 1 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In a previous study , we described a coexpression of KIT and stem cell factor ( SCF ) mRNA in Synovial sarcomas , ( SS ) and in a limited number of cases , we demonstrated the presence of an activated receptor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
This small molecule , tyrosine kinase inhibitor , has also been shown to be effective against metastatic gastrointestinal stromal tumors ( GISTs ) expressing the stem cell factor ( SCF ) receptor kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
SCF is a potent pro proliferative cytokine crucial for haematopoiesis , which binds to c kit and activates its tyrosine kinase activity . ^^^ Inactivating mutations of either SCF or c kit have been described in mice and lead to increased sensitivity to treatment with ionising radiation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Aggregation of high affinity receptors for immunoglobulin E ( Fc epsilon RI ) on the surface of mast cells results in degranulation , a response that is potentiated by binding of stem cell factor ( SCF ) to its receptor Kit . ^^^ Fc epsilon RI and SCF mediated NTAL phosphorylation appear to be differentially regulated by Src kinases and / or Kit kinase , respectively . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The Kit receptor tyrosine kinase is a transmembrane receptor that is expressed in a variety of different tissues and mediates pleiotropic biological effects through its ligand stem cell factor ( SCF ) . ^^^ Sporadic mutations of Kit as well as autocrine / paracrine activation mechanisms of the SCF / Kit pathway have been implicated in a variety of malignancies , where its primary contribution to metastases is in enhancing tumor growth and reducing apoptosis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mechanisms underlying the dysfunction of melanocytes in vitiligo epidermis : role of SCF / KIT protein interactions and the downstream effector , MITF M . ^^^ This study has compared the expression of endothelin ( ET ) 1 , the ET 1 receptor ( ET ( B ) R ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , stem cell factor ( SCF ) , the SCF receptor ( KIT protein ) , tyrosinase , and S 100 alpha between lesional and non lesional vitiligo epidermis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
OBJECTIVE : Stem cell factor ( SCF ) is the ligand for the receptor tyrosine kinase ( RTK ) Kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Haematopoietic stem cells ( HSC ) express CD 34 , the adhesion structure which binds 2L selectin , CD 117 , a receptor for stem cell factor ( SCF ; c kit ligand ) , and CD 133 , a transmembrane protein belonging to the family of mucoproteins . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) / Kit and insulin like growth factor 1 ( IGF 1 ) / IGF 1 receptor ( IGF IR ) autocrine loops play a prominent role in the growth of small cell lung cancer ( SCLC ) . ^^^ SCF mediated Kit phosphorylation and Akt activation were inhibited with IC ( 50 ) values in the 1 5 micro M range . ^^^ One population , which lacks active SCF / Kit autocrine loops , was inhibited with IC ( 50 ) values between 0 . 1 and 0 . 5 micro M . ^^^ A second population , which has active SCF / Kit autocrine loops , was inhibited with IC ( 50 ) values in the 4 7 micro M range . ^^^ However , for optimal growth inhibition of SCLC cells with an active SCF / Kit autocrine loop , a combination of a Kit inhibitor ( STI 571 ) and an IGF IR inhibitor ( NVP ADW 742 ) appears to be necessary . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The inhibition by STI 571 appeared at least to some extent independent of c Kit inhibition since cells remained sensitive to SCF stimulation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
LPA accelerated the acquisition of hMC granules and increased Kit expression . hMCs derived in the presence of LPA were functional , as evidenced by their immunoglobulin E ( IgE ) dependent histamine release and by their characteristic proliferative responses to interleukin 3 ( IL 3 ) , IL 4 , and IL 9 in combination with SCF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The inhibitory effect of STI 571 suggests a role for c kit tyrosine kinase on glucose transport activation not only by SCF , but also by H2O2 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In unstimulated cells , mRNA for the stem cell factor ( SCF ) receptor c kit and the gamma chain of the high affinity IgE receptor ( FcepsilonRI ) was noted in all cells studied . ^^^ After a 10 day culture with SCF or fibroblast supernatants , baseline mRNA expression of most mast cell characteristics was upregulated , whereas c kit mRNA expression decreased in all but THP 1 cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Accordingly , we found that Stem Cell Factor ( SCF ) activated c Kit induces phosphorylation of the IL 2Rbeta chain in the absence of IL 2 . ^^^ Moreover , inhibition of IL 2Rbeta phosphorylation by blocking c Kit tyrosine kinase activity abolishes both , IL 2 and SCF mediated proliferation . ^^^ Thus , these results demonstrate that IL 2 triggers a JAK3 / STAT5 cascade in HPV associated cervical cancer cells expressing IL 2Rbetagamma , and that this receptor can be alternatively activated by SCF activated c Kit in the absence of IL 2 . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Here , we identified a pathway that involves stem cell factor ( SCF ) / c Kit / Slug in mediating multidrug resistance of MM cells . ^^^ When we compared gene expression profiles between five MM cells and their multidrug resistant ( MM DX ) sublines , we found that MM DX cells expressed both SCF and c Kit and had higher mRNA levels of Slug . ^^^ Transfection of c Kit in parental MM cells in the presence of SCF up regulated Slug and increased resistance to the chemotherapeutic agents . ^^^ These results indicate that induction of Slug by autocrine production of SCF and c Kit activation plays a key role in conferring a broad spectrum chemoresistance on MM cells and reveal a novel signal transduction pathway for pharmacological or genetic intervention of MM patients . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Several lines of evidence have previously reported that KIT and its ligand stem cell factor ( SCF ) have a regulatory role in melanocyte development and survival , suggesting a rational mechanism of action for imatinib mesylate in the pathogenesis of hypopigmentation . ^^^ The signal transduction mechanism currently is believed to involve SCF ligand binding of KIT and downstream activation of MAP kinase ( Erk 2 ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Ecotropic retroviral particles produced in cells engineered to express the membrane bound form of stem cell factor ( mbSCF ) transduce both human cell lines and primary cells with high efficiency in a strictly c kit ( SCF receptor ) dependent fashion . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
RESULTS : Retroviruses incorporating surface SCF showed increased levels of binding to cells ( MO7e ) expressing the SCF receptor , c kit . mbSCF displayed on the viral surface retained levels of biological activity comparable with those of soluble recombinant growth factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We previously reported that subcutaneous ( s . c . ) administration of stem cell factor ( SCF ) , the ligand for the c Kit receptor , to the back skin of mice promotes marked local increases in the numbers of cutaneous mast cells ( MCs ) , and that cessation of SCF treatment results in the rapid reduction of cutaneous MC populations by apoptosis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Modulation of c Kit / SCF pathway leads to alterations in topoisomerase 1 activity in small cell lung cancer . ^^^ The ligand for c Kit is stem cell factor ( SCF ) . ^^^ In SCLC , SCF can influence c Kit activation by autocrine or paracrine mechanisms . ^^^ We have recently shown that the c Kit / SCF pathway is operational in SCLC and can be inhibited by Glivec ( STI 571 ) . ^^^ Because the inhibition of topoisomerase 1 ( topo 1 ) is one approach used to treat SCLC , we determined the effects of c Kit / SCF signaling on topo 1 activity . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Therefore , it seemed that SCF initiated an anti apoptotic signal starting from its membrane receptor c kit to Bcl 2 family members through PI3K / AKT and other signaling cascades in the oocytes of primordial follicles . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
High resolution tracking of cell division demonstrates differential effects of TH 1 and TH 2 cytokines on SCF dependent human mast cell production in vitro : correlation with apoptosis and Kit expression . ^^^ To determine the effect of these cytokines on stem cell factor ( SCF ) dependent human mast cell production , we used high resolution tracking of cell division and correlated the results with cell apoptosis , expression of Kit , and mast cell degranulation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
SCF induces autophosphorylation of Kit and activates a variety of signaling components including Jnks , Erks , PI 3 Kinase , the JAK Stat pathway and members of the Src family . ^^^ Previously we showed that Lyn is activated at multiple points during SCF induced cell cycle progression and contributes to SCF mediated growth , chemotaxis and internalization of Kit . ^^^ In this study , we used Lyn deficient bone marrow mast cells ( BMMC ) to examine the contribution of this Src family member to tyrosine phosphorylation of Kit and SCF induced activation of Jnks , Akt , Stat 3 and Erks . ^^^ Although surface expression of Kit was increased in Lyn deficient BMMC , SCF induced phosphorylation and growth was reduced compared to wild type BMMC . ^^^ Downstream of Kit , SCF induced activation of Jnks was markedly reduced in Lyn deficient BMMC . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In IL 3 dependent Ba / F3 cells expressing c kit , forced expression of Spred 1 resulted in a reduced proliferation rate and ERK activation in response to not only SCF but also IL 3 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The SCF receptor , Kit , was expressed in neural stem cells and in their differentiated progeny . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
METHODS : We identified murine ICCs in live gastrointestinal muscles or primary cell cultures grown in the presence or absence of stem cell factor ( SCF ) expressing STO fibroblasts with fluorescent Kit ( CD 117 ) antibodies . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
PKCdelta is activated after wild type ( WT ) Kit binds stem cell factor ( SCF ) , is constitutively active in cells expressing the Kit catalytic domain mutant , and coprecipitates with both forms of Kit . ^^^ Both rottlerin and a dominant negative PKCdelta construct inhibited the growth of cells expressing mutant Kit , while SCF induced growth of cells expressing wild type Kit was not inhibited . ^^^ These data demonstrate that PKCdelta contributes to factor independent growth of cells expressing the D814Y mutant , but negatively regulates SCF induced growth of cells expressing wild type Kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The receptor , c kit , and its ligand , stem cell factor ( SCF ) , are important regulators of ovarian follicle growth and development . ^^^ The aim of this study was to identify the sites of expression of mRNA for c kit and SCF in prepubertal and mature ( pregnant and non pregnant ) animals . ^^^ Ovine SCF and c kit DNA were cloned into plasmid vectors to produce RNA probes . ^^^ Expression of mRNA encoding SCF and c kit were detected via in situ hybridization . ^^^ SCF mRNA was localized to the granulosa cell layer and was also extensively expressed in endothelial tissue and throughout the CL . c kit mRNA was detected in the theca layer , oocytes and also in CL . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
When human forearm skin was exposed to UVB radiation at two minimal erythemal doses , the expression of SCF mRNA transcripts was significantly enhanced at 3 days after irradiation with an early decrease and subsequently constant expression of SCF receptor ( c KIT ) mRNA transcripts . ^^^ These findings suggest that SCF / KIT signaling is predominantly involved in the early phase of UVB induced human pigmentation during which it stimulates the ET 1 / ET ( B ) R linkage that is associated with the later phase of UVB induced melanogenesis . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Compared with serum and multiple growth factor containing medium , cells cultured for 4 weeks in AF / SCF medium displayed downregulation of differentiation markers while maintaining a high fraction of cells expressing Sca 1 ( 51 . 8 % ) and c kit ( 10 . 2 % ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
All KIT exon 8 mutants induced receptor hyperactivation in response to stem cell factor ( SCF ) stimulation in terms of proliferation and resistance toward apoptotic cell death . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Binding of stem cell factor ( SCF ) to c kit receptor tyrosine kinase ( KIT ) transduces signals essential for mast cell development via several pathways including activation of phosphatidylinositol 3 kinase ( PI 3 K ) . ^^^ When cultured mast cells ( CMCs ) are cocultured with fibroblasts expressing membrane bound SCF , CMCs with normal KIT adhere to fibroblasts and proliferate , whereas CMCs lacking cell surface expression of KIT do neither . ^^^ The effect was abolished by an antibody that blocks SCF KIT interaction . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) and its receptor , KIT , are essential to the migration and differentiation of melanocytes during embryogenesis . ^^^ We previously demonstrated that apoptosis is induced by blocking survival function of the SCF / KIT interaction in a mouse neural crest cell ( NCC ) primary culture . ^^^ Furthermore , we hypothesize that these molecules combined with SCF / KIT play an important role in regulating the induction of vertebrate NCC apoptosis during embryogenesis . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
PURPOSE : Clinicobiological characteristics of neuroblastic tumor ( NT ) expressing c kit tyrosine kinase receptor and / or its ligand , stem cell factor ( SCF ) , are debated . ^^^ This study aimed at investigating the clinicobiological features of primary NTs expressing c kit and / or SCF in order to define the clinical relevance of selective therapeutic targeting . ^^^ EXPERIMENTAL DESIGN : c Kit and SCF expression was studied in 168 NTs using immunohistochemistry and in 106 of 168 using Northern blot . ^^^ Correlations between c kit and SCF expression and clinicobiological features were analyzed using chi 2 test , univariate , and multivariate regression analyses . ^^^ By univariate analysis , c kit and SCF expression correlated with advanced stage , MYCN amplification , and 1p36 allelic loss . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The KTHOS cells were immunoreactive for osteocalcin , osteonectin , stem cell factor ( SCF ) , and KIT ( CD 117 ) . ^^^ Reverse transcriptase polymerase chain reaction indicated that the KTHOS cell line expressed mRNA for SCF and KIT . ^^^ The results suggest that cell proliferation of the KTHOS cell line might be involved in autocrine and / or paracrine loops of the SCF / KIT signaling system . ^^^ The KTHOS cell line is a novel human osteosarcoma cell line that releases SCF and expresses KIT . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Here we investigated the expression of stem cell factor ( SCF ) , a specific ligand of c kit , in CNS germ cell tumor samples from 16 patients , using immunohistochemical methods to assay the expression of c kit and SCF protein . ^^^ The immunostaining patterns of c kit and SCF were almost identical . ^^^ In all germinoma containing tumors , c kit and SCF were diffusely expressed on the surface of germinoma cells ; lymphocytes and interstitial cells were negatively stained . ^^^ In immature teratomas , only some mature components , e . g . , cartilage and gland , were immunoreactive for c kit and SCF . ^^^ Syncytiotrophoblastic giant cells ( STGCs ) were negative for both SCF and c kit , suggesting that germinoma cells primarily coexpress SCF and c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In vitro , in the presence of interleukin 3 ( IL 3 ) and stem cell factor ( SCF ) these cells differentiate only into a granulated mast cell that now expresses CD 13 , c kit , mast cell specific gangliosides , FcepsilonRI , and binds immunoglobulin E ( IgE ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Analysis of KIT , SCF , and initial screening of SLUG in patients with piebaldism . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
No or little inhibitory effect of SCF was detected in BMCMC derived from c kit receptor defect WBB6F1 W / Wv mice . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Viable mice with mutations in the SCF gene ( encoded by the Steel ( Sl ) locus ) , or its receptor gene c Kit ( encoded by the White spotting ( W ) locus ) develop a hypoplastic macrocytic anemia . ^^^ Mutants of W or Sl that are completely devoid of c Kit or SCF expression die in utero of anemia between days 14 and 16 of gestation and contain reduced numbers of erythroid progenitors in the fetal liver . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Selective inhibitor of ERK , PD 098059 , and c kit inhibitors , STI 571 and PP 1 , suppressed the combined SCF and TNF alpha induced ICAM 1 expression . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The distribution of the tyrosine kinase receptor c Kit and its ligand stem cell factor ( SCF ) was evaluated by immunohistochemistry in primordial germ cells ( PGCs ) and human embryonic gonads during weeks 5 8 of prenatal life , and fetal ovaries during weeks 9 36 of prenatal life . ^^^ Distinct c Kit and SCF staining was present in primordial germ cells in the wall of the hindgut and in the dorsal mesentery , particularly on level with the 10th thoracic columnar segment . ^^^ Several PGCs were in close contact with c Kit negative but SCF positive autonomic nerve fibers of the dorsal mesentery . ^^^ Many fibroblasts and mesothelial cells of the dorsal mesentery were clearly stained for SCF , but not for c Kit . ^^^ Prominent c Kit and SCF staining was present in germ cells of the embryonic gonadal anlage and in oogonia during further ovarian development . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In addition , cellulose surface presentation of the SCF CBD attenuates c kit dephosphorylation kinetics , potentially modulating the overall response of the cell to the SCF signal . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The receptor tyrosine kinase c KIT and its ligand Stem Cell Factor ( SCF ) are critical in haemopoiesis but pathways linking receptor activation to specific responses in progenitor cells are still unclear . ^^^ We have investigated the role of c KIT expression level and the phosphatidylinositol 3 kinase ( PI 3 K ) pathway in survival and cell division of early myeloid cells in response to SCF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Although communications between mammalian oocytes and their surrounding granulosa cells mediated by the Kit Kit ligand ( KL , or stem cell factor , SCF ) system have been proven to be crucial for follicular development , Kit downstream signaling pathways in mammalian oocytes are largely unknown . ^^^ We propose that the cascade from granulosa cell SCF to oocyte Kit PI 3 kinase Akt FKHRL 1 may play an important role to regulate the growth rate of mammalian oocytes and hypothetically also the oocyte secretion of factors that may regulate the activation and early development of ovarian follicles . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
These Stem Cell Factor ( SCF ) and Interleukin 3 ( IL 3 ) dependent PMC lines retain their capacity to growth after conventional freezing methods and exhibit most of the morphological and biochemical properties of normal , although immature , MCs , including metachromatic granules containing sulfated polysaccharides , the expression of c kit and high affinity IgE receptors ( FcepsilonRI ) , and the ability to store histamine that is released upon cross linking of FcepsilonRI . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In order to investigate the levels of stem cell factor ( SCF ) and its receptor c kit protein and mRNA in pediatric aplastic anemia ( AA ) and their relevance to the pathogenesis , immunocytochemical and in situ hybridization were utilized to detect the expression of SCF and its receptor c kit gene protein and mRNA , respectively in 59 children with AA and 51 normal controls . ^^^ The relationship between SCF and c kit and the pathogenesis of AA was analyzed subsequently . ^^^ It was concluded that the expression of SCF is significantly decreased in children with AA , which may be closely associated with the pathogenesis of the AA . c kit may be unrelated to the development of pediatric AA . ^^^ Therefore , AA in children may have abnormalities at SCF / c kit signal transduction levels . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Expression of SCF protein was increased at 4 , 7 , and 9 days by TPO gene administration and that of c Kit was increased at 4 and 7 days . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
While stem cell factor ( SCF ) is abundant in the CNS and is chemotactic to mast cells in the periphery , thalamic mast cells in the rodent do not express c kit , the SCF receptor , suggesting that this factor may not be responsible for the effect . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Patients with tumours expressing c kit tend to exhibit better survival , but a paracrine / autocrine function for the stem cell factor ( SCF ) / c kit system in nasopharyngeal carcinomas has not been reported . ^^^ This study evaluated the expression of c kit and SCF by immunohistochemical staining of nasopharyngeal epithelium ( NPE ) and of primary and metastatic NPCs . ^^^ In addition , c kit and SCF expression were studied in HONE 1 NPC cells by immunoprecipitation and western blotting . ^^^ Expression of c kit and SCF was detected in 75 % and 57 % of NPE , respectively , and there was 48 % co expression . ^^^ In primary NPCs , 86 % expressed c kit , 69 % had SCF expression , and there was 67 % co expression . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Kinetic tracking of IL 3alpha receptor ( CD 123 ) and SCF receptor ( CD 117 ) expression on a sorted CD 45 cell population revealed the emergence of CD 45 CD123+ cells capable of osteogenesis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Expression of the serine proteases tryptase and chymase , as well as KIT protein , the receptor for stem cell factor ( SCF ) , has been demonstrated in mast cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) kit mediated phosphatidylinositol 3 ( PI 3 ) kinase signaling during mammalian oocyte growth and early follicular development . ^^^ We propose that the cascade from the granulosa cell produced stem cell factor ( SCF ) to the oocyte surface SCF receptor Kit , and to the oocyte PI 3 kinase pathway , may play an important role in the regulation of growth rate of mammalian oocytes , as well as in the activation and development of ovarian follicles . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Here , we show that bone morphogenetic protein type 2 receptor ( BMPR 2 ) which has a serine / threonine kinase domain , and stem cell factor receptor ( c kit ) which contains a tyrosine kinase domain form a complex in vitro and in vivo ; the interaction is induced upon treatment of cells with BMP 2 and SCF . ^^^ Abrogation of the c kit / SCF dependent phosphorylation of BMPR 2 at the Ser 757 interfered with the cooperative effect of BMP 2 and SCF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In the present study , we demonstrate a neuroprotective role for SCF and its tyrosine kinase receptor , c kit , against camptothecin induced apoptosis and glutamate excitotoxicity in rat cortical neurons . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Although stem cell factor ( SCF ) and its receptor c kit are constitutively expressed on ASM cells and mast cells , respectively , neutralizing antibodies to SCF and c kit failed to inhibit ASM cell mediated enhancement of mast cell degranulation . ^^^ These findings suggest that cell cell interaction between ASM cells and mast cells , via a SCF c kit independent but dexamethasone sensitive mechanism , enhances C3a induced mast cell degranulation , which likely regulates ASM function , thus contributing to the pathogenesis of asthma . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
SCF synergized with chemoattractants in causing basophil upregulation of the integrin CD11b , and this effect was inhibited by a c kit antibody , the tyrosine kinase inhibitor imatinib mesylate ( STI 571 ) , and a phosphatidylinositol 3 kinase inhibitor but not by inhibitors of p 38 mitogen activated protein kinase or mitogen activated protein kinase / extracellular signal regulated kinase kinase . ^^^ Basophils bound fluorescence labeled SCF and expressed its receptor , c kit , which was markedly upregulated in culture for 24 to 48 hours in the presence of IL 3 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
KIT is the receptor for stem cell factor ( SCF ) and required for normal hematopoiesis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , a progenitor cell growth factor , binds to and activates the c Kit receptor tyrosine kinase , which is critical for early stem cell differentiation in haematopoiesis and gametogenesis . ^^^ The present study was to investigate whether an increase in c Kit receptor activity in isolated human fetal islet epithelial clusters , by giving exogenous SCF , would promote beta cell development . ^^^ In the intact fetal pancreas , SCF and c Kit were observed co localizing with cytokeratin 19 in both ductal and newly forming islet cells . ^^^ We observed an increase in the number of c Kit , pancreatic and duodenal homeobox gene 1 ( PDX 1 ) , insulin and glucagon expressing cells in the SCF treated group ( PDX 1 and insulin , p < 0 . 05 ) . ^^^ PDX 1 and c Kit mRNA levels were also up regulated in the SCF group ( PDX 1 , p < 0 . 05 ) , with no change in preproinsulin or proglucagon gene expression . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Notably , all HF MCs showed immunoreactivity for Kit , the receptor for the MC growth and maturation factor SCF , as assessed by FITC avidin / Kit double labelling . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) stimulation of the receptor tyrosine kinase c kit has effects on the proliferation , differentiation , and apoptotic regulation of hematopoietic progenitor cell populations . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Herein , we report the presence of acquired mutations in the SCF receptor gene ( Kit ) in 86 % of tumors isolated during the late stage of the disease . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Both populations contain similar levels of stem cell factor ( SCF ) receptor ( c Kit ) but only the CD34+ population shows a growth response when treated with SCF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Molecular characterization of PDGFR alpha / PDGF A and c KIT / SCF in gliosarcomas . ^^^ Six cases were analyzed by immunohistochemistry for the expression of PDGFR alpha , c Kit and their ligands PDGF A and SCF , respectively . ^^^ Four cases showed expression of SCF , and c Kit was observed only in one case that also expressed SCF . ^^^ Nevertheless , the presence of a PDGFR a / PDGFA and c Kit / SCF autocrine / paracrine stimulation loop in a proportion of cases , supports the potential role of specific tyrosine kinase inhibitors in the treatment of gliosarcomas . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
SCF downregulation in the epidermis is concomitant with the dramatic reduction in Mb numbers observed in the Bcl 2 null , suggesting that Bcl 2 is indispensable for the survival of Mbs in the absence of c Kit signaling . ^^^ Consistently , abrogation of c Kit signaling in Bcl 2 null mice depletes all Mbs and Mcs , whereas continuous expression of SCF in epidermal keratinocytes rescues the MSCs . ^^^ Furthermore , a Bcl 2 independent mechanism of action of SCF in the Mc lineage is revealed as SCF c Kit signaling is functional in the absence of Bcl2 . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , the ligand of the c Kit receptor , is expressed by various structural and inflammatory cells in the airways . ^^^ Binding of SCF to c Kit leads to activation of multiple pathways , including phosphatidyl inositol 3 ( PI 3 ) kinase , phospholipase C ( PLC ) gamma , Src kinase , Janus kinase ( JAK ) / Signal Transducers and Activators of Transcription ( STAT ) and mitogen activated protein ( MAP ) kinase pathways . ^^^ Inhibition of the SCF / c Kit pathway leads to significant decrease of histamine levels , mast cells and eosinophil infiltration , interleukin ( IL ) 4 production and airway hyperresponsiveness in vivo . ^^^ Taken together , these data suggest that SCF / c Kit may be a potential therapeutic target for the control of mast cell and eosinophil number and activation in inflammatory diseases . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
For example , activation of Kit , a transmembrane receptor for stem cell factor ( SCF ) is critical for the growth and differentiation of normal mast cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Co expression of SCF and KIT in gastrointestinal stromal tumours ( GISTs ) suggests an autocrine / paracrine mechanism . ^^^ KIT is a tyrosine kinase receptor expressed by several tumours , which has for specific ligand the stem cell factor ( SCF ) . ^^^ Thus , KIT activation in GISTs may in part result from the presence of SCF within the tumours . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
OBJECTIVE : To express the first three immunoglobulin like domains of human stem cell factor receptor ( c Kit / Ig1 3 ) in E . coli and HEK 293 ET cells and study their binding activity for stem cell factor ( SCF ) . ^^^ RESULTS : In E . coli c Kit / Ig1 3 ( DM ) as produced as an inclusion body and showed low binding activity for SCF after refolding . ^^^ Eukaryotically expressed c Kit / Ig1 3 had specific binding activity for SCF , and the dissociation constant ( Kd ) was 9 . 39 nmol / L . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Despite the relevance of the c kit / stem cell factor ( SCF ) signaling pathway in mast cell ( MC ) diseases , the exact frequency of KIT mutations in different compartments of bone marrow ( BM ) hematopoietic cells of individuals with systemic mastocytosis ( SM ) , and its different diagnostic categories , remains unknown . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
OBJECTIVE : To evaluate canine histiocytic sarcoma cell lines and tumor samples for dysregulation of the Kit / stem cell factor ( SCF ) , Flt3 / Flt3 ligand ( Flt3L ) , and Met / hepatocyte growth factor ( HGF ) receptor tyrosine kinase signaling pathways , as these are known to contribute to the differentiation and survival of normal dendritic cells as well as malignant transformation of dendritic cells in mouse models . ^^^ PROCEDURE : Histiocytic sarcoma cell lines were evaluated for expression of Kit / SCF , Flt3 / Flt3L , and Met / HGF by use of reverse transcriptase PCR procedures . ^^^ CONCLUSIONS AND CLINICAL RELEVANCE : These data suggest that dysregulation of Kit / SCF , Flt3 / Flt3L , and Met / HGF signaling pathways is unlikely to occur in histiocytic sarcomas of dogs and that inhibitors of the Kit , Flt 3 , and Met pathways are unlikely to provide clinical benefit to dogs with histiocytic sarcomas . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We tried to determine whether the stem cell factor ( SCF ) and its receptor c kit are involved in the mechanism of hyperpigmentation of melasma because this factor is highly implicated in the stimulation of melanocyte function in vitro and in vivo . ^^^ OBJECTIVES : The present study was conducted to investigate the expression of SCF and c kit on the lesions of melasma compared with nonlesional skin . ^^^ Immunohistochemistry and reverse transcriptase polymerase chain reaction ( RT PCR ) analysis was performed to determine the expression of SCF and c kit in melasma . ^^^ RT PCR of SCF and c kit mRNAs demonstrated increased expression of both types of transcripts in the lesional skin compared with nonlesional skin . ^^^ CONCLUSIONS : These results suggest that the increased expression of SCF in the dermis and of c kit in the epidermis play an important role in the mechanism of hyperpigmentation in melasma . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We used mature MC from human skin , along with the MC lines LAD 2 and HMC 1 and treated them with stem cell factor ( SCF ) , cycloheximide , actinomycin D ( AD ) and combinations thereof , and determined expression levels of c Kit and other surface receptors by flow cytometry . ^^^ By Western blot analysis of LAD 2 cells , c Kit was found to nearly disappear 3 h after the addition of SCF to slowly recover thereafter . ^^^ On combined treatment , cycloheximide , AD and SCF displayed additive effects , resulting in a complete disappearance of c Kit from the cell surface . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The expression of SCF receptor Kit was assessed using immunostaining of paraffin embedded bronchial sections . ^^^ CONCLUSION : SCF and Kit are expressed in bronchial biopsies from lung transplant recipients irrespective of the clinical status of the graft . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The interaction between the cytokine stem cell factor ( SCF ) and its cognate receptor , the c kit receptor tyrosine kinase ( KIT ) , plays a central role in regulating MC growth and differentiation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In addition to hypoxia , peptide growth factors are known to regulate VEGF expression but the effect of stem cell factor ( SCF ) , the ligand for c Kit , on VEGF expression has not been characterized . ^^^ We therefore studied the effect of SCF mediated c Kit activation on VEGF expression by the H 526 small cell lung cancer ( SCLC ) cell line . ^^^ These data indicate that activation of c Kit by SCF leads to a predominantly HIF 1alpha mediated enhancement of VEGF expression and that inhibition of c Kit signaling with imatinib could result in inhibition of tumor angiogenesis . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We hypothesized that the stem cell factor ( SCF ) / c Kit tyrosine kinase signaling pathway is critical to neointimal formation by BM derived progenitors . ^^^ To characterize the role of the SCF / c Kit pathway , we used c Kit deficient W / W ( 5 ) and SCF deficient Steel Dickie mice . ^^^ Strikingly , vascular injury in these mice resulted in almost a complete inhibition of neointimal formation , whereas wild type BM reconstitution of c Kit mutant mice led to neointimal formation in a similar fashion as wild type animals , as did chronic administration of SCF in matrix metalloproteinase 9 deficient mice , a model of soluble SCF deficiency . ^^^ Pharmacological antagonism of the SCF / c Kit pathway with imatinib mesylate ( Gleevec ) or ACK 2 ( c Kit antibody ) also resulted in a marked reduction in intimal hyperplasia . ^^^ Vascular injury resulted in the local upregulation of SCF expression . c Kit ( + ) progenitor cells ( PCs ) homed to the injured vascular wall and differentiated into alphaSMA ( + ) cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The Rh 123lo population was determined to be more primitive than Rh 123hi based on the expression of stem cell markers such as the c kit protooncogene ( stem cell factor receptor ) and the Ly 6A / E stem cell antigen ( Sca 1 ) as well as the lack of in vitro colony forming ability . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In this study , recombinant human stem cell factor ( rhuSCF ) , the ligand for the c kit proto oncogene product called Kit , stimulated the growth and differentiation primarily of mast cells from dispersed fetal liver cells , whereas recombinant human interleukin 3 ( rhuIL 3 ) stimulated the differentiation of basophils along with other cell types . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In contrast , KIT antisense oligomers inhibited interleukin 3 / erythropoietin driven erythroid colony formation approximately 70 % and `` stem cell factor ' ' / erythropoietin driven colony formation 100 % . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Lack of apparent hematologic abnormalities in human patients with c kit ( stem cell factor receptor ) gene mutations . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Previous studies from ours and other laboratories suggested that mast cell differentiation involved the W allele product , c kit , as a receptor and Sl allele product , stem cell factor , as a growth factor . ^^^ The results suggest that c kit and its ligand , stem cell factor , are necessary for mast cell committed progenitors to proliferate and granulate in response to F CM but not IL 3 . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Recombinant human stem cell factor stimulates growth of a human glioblastoma cell line expressing c kit protooncogene . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We have studied the effect of the human recombinant c kit receptor ligand stem cell factor ( rhSCF ) on the release of inflammatory mediators from human skin mast cells and peripheral blood basophils and compared its activity to that of rhIL 3 , rhSCF ( 1 ng / ml to 1 microgram / ml ) activated the release of histamine and PGD 2 from mast cells isolated from human skin . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
CD25+ TN thymocytes proliferated in the presence of IL 7 and stem cell factor ( the ligand for c kit ) , and this proliferation was completely inhibited in the presence of anti c kit . ^^^ These observations indicate an important role for c kit / stem cell factor interactions during early thymocyte development . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The W locus encodes the c kit protein tyrosine kinase receptor whose ligand is the stem cell factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Some of these are influenced by action of cytokines at the cell surface , an example of which is the interaction of c kit with its ligand , the stem cell factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The kit protooncogene encodes a transmembrane tyrosine kinase related to the receptors for the platelet derived growth factor ( PDGF R ) and the macrophage growth factor ( CSF 1 R ) , and was very recently shown to bind a stem cell factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In 6 of 11 cases that expressed c kit product , AML cells were found to proliferate in response to recombinant human stem cell factor ( rhSCF ) , the ligand for c kit , and the synergistic stimulation of AML cells was observed by rhSCF and granulocyte macrophage colony stimulating factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
By contrast , the stem cell factor receptor ( SCFR ) , which is encoded by c kit , is essential for normal mast cell development . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The c kit proto oncogene encodes a tyrosine kinase receptor for stem cell factor and plays a critical role in the growth and differentiation of various types of cells including hematopoietic stem cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Ligand induced dimerization is a key step in the activation of receptor tyrosine kinases , including the epidermal growth factor receptor , stem cell factor receptor ( c kit ) , and colony stimulating factor 1 receptor ( c fms ) . ^^^ Here we show that chimeric receptors carrying the extracellular domain of the epidermal growth factor receptor or c kit linked to the cytoplasmic domain of the EPOR , transmitted epidermal growth factor or stem cell factor dependent proliferation signals in an interleukin 3 dependent cell line . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Although the proliferative response of rat cultured mast cells ( RCMC ) derived from Ws / Ws rats to ConA SCM was comparable to that of RCMC derived from control normal ( + / + ) rats , the proliferative response of Ws / Ws RCMC to rat recombinant stem cell factor ( rrSCF ; a ligand for the c kit receptor tyrosine kinase ) was much lower than that of + / + RCMC . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Four lines of evidence suggest that most are basophils : ( 1 ) The cells fulfill morphologic criteria for light microscopy . ( 2 ) Cell surface markers determined by immunofluorescence and flow cytometry revealed that the IgE bearing cells express the leukocyte antigens Fc gamma RII and the beta 2 integrins , LFA 1 and Mac 1 , but do not express the mast cell associated c kit receptor for stem cell factor . ( 3 ) The late phase histamine containing cells in late phase BAL fluids have the functional characteristics of basophils in their secretory responses to anti IgE , the f met peptide , and phorbol ester TPA . ( 4 ) The cells have a functional histamine type 2 receptor , a characteristic of basophils , not mast cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We demonstrate that rat oligodendrocyte type 2 astrocyte ( O 2A ) glial progenitor cells , precursors of the myelin forming cells in the CNS , express the transmembrane RTK c kit , the gene product of the murine dominant white spotting ( W ) locus and receptor for stem cell factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In the transfected cells , mutant form KITTyr 814 was strikingly phosphorylated on tyrosine and activated in immune complex kinase reaction regardless of stimulation with a ligand for KIT ( stem cell factor ) , whereas tyrosine phosphorylation and activation was barely detectable in wild form KIT . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Role for the stem cell factor / KIT complex in Schwann cell neoplasia and mast cell proliferation associated with neurofibromatosis . ^^^ The receptor for stem cell factor , KIT , was not expressed in normal Schwann cells but was expressed in the human Schwannoma line , suggesting that aberrant KIT expression may form an autocrine loop in certain Schwann cell neoplasias . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Besides the soluble factors , cell to cell contacts mediated by membrane stem cell factor on splenic stromal cells and c Kit receptors on mast cells also affect mast cell induction . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We assessed the expression of the adhesion molecules leukocyte function antigen 1 ( LFA 1 , CD11a ) , intercellular adhesion molecule 1 ( ICAM 1 , CD 54 ) , homing associated cell adhesion molecule ( H CAM , CD 44 ) , and c kit ( stem cell factor receptor ) on the CD34+ progenitor population from the leukapheresis products of 23 patients ( LP CD34+ ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor is a recently identified earliest acting hematopoietic growth factor and a ligand for the c kit proto oncogen . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Synergistic actions of stem cell factor and other burst promoting activities on proliferation of CD34+ highly purified blood progenitors expressing HLA DR or different levels of c kit protein . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
This effect is synergized by stem cell factor , the ligand of the tyrosine kinase receptor encoded by the c KIT protooncogene , which is active on early hemopoietic progenitors . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
All reagents stained normal skin mast cells , with toluidine blue , tryptase reactivity and antibodies against the Fc epsilon RI and the stem cell factor receptor ( c kit ) being most active . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Flow cytometric analysis of five myeloma cell lines ( RPMI 8226 , ARH 77 , KMM 1 , U 266 , and Hs ) and myeloma cells freshly isolated from eight patients showed that interleukin 1 receptor ( IL 1R ) type 1 and type 2 , IL 2R alpha and beta chains , IL 4R , IL 6R , IL 7R , IL 8R , granulocyte macrophage colony stimulating factor receptor ( GM CSFR ) , c kit ( stem cell factor receptor [ SCFR ] ) , membrane bound stem cell factor ( MBSCF ) , and tumor necrosis factor ( TNF ) receptors type 1 and type 2 were not always detected on the myeloma cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The receptors studied were interleukin ( IL ) 1 receptor type 1 ( IL 1RI ) and type 2 ( IL 1RII ) , p 75 and p 55 tumor necrosis factor ( TNF ) receptors ( p75TNFR and p55TNFR ) , interferon ( IFN ) alpha / beta and gamma receptors ( IFN alpha / beta R and IFN gamma R ) , granulocyte macrophage ( GM ) colony stimulating factors receptor alpha subunit ( GM CSFR ) , G CSF receptor ( G CSFR ) , M CSF receptor ( c fms , M CSFR ) , stem cell factor receptor ( c kit , SCFR ) , IL 6 receptor ( IL 6R ) , and IL 8 receptor ( IL 8R ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Burst forming units erythroid ( BFU E ) response to stem cell factor and c kit receptor expression . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Furthermore , the addition of stem cell factor stimulated the cells to increase the amount of c kit mRNA transcribed in response to its ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The c kit receptor transduces the stem cell factor triggered growth signal in murine interleukin 3 dependent cell line . ^^^ The stem cell factor is a glycoprotein hormone which regulates the proliferation and differentiation of primitive hematopoietic cells through its interaction with a tyrosine kinase transmembrane receptor which is encoded by the c kit proto oncogene . ^^^ One of the resulting clones , Ba / F3 clone BF K 96 , expressed the 140 kDa protein recognized by anti c kit monoclonal antibody and the expressed c kit receptor protein on the cell surface bound to a radiolabeled soluble form of murine stem cell factor ( mSCF ) with high affinity . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In the culture containing both ConA SCM and stem cell factor ( a ligand of c kit ) , however , the numbers of mast cell colonies produced by + / + blood MNC were 107 times as great as that of Ws / Ws blood MNC . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
These findings demonstrated that the significant expansion of human primitive hemopoietic progenitors could be achieved with the gp 130 and c Kit signalings initiated by the sIL 6R / IL 6 complex in the presence of stem cell factor and suggested the possible application of this method for ex vivo expansion of CD34+ cells for bone marrow transplantation . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The receptor for the stem cell factor encoded by the c kit proto oncogene is expressed by a number of epithelial cells including thyrocytes . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The proto oncogene c kit encodes the receptor for a stem cell factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Coexpression of the c kit and stem cell factor genes in breast carcinomas . ^^^ Expression of the c kit tyrosine kinase growth factor receptor has been reported in some breast tumors ; however , no data exist concerning expression of its ligand , stem cell factor . ^^^ The aim of this study was to determine how frequently the c kit and stem cell factor genes were coexpressed in breast tumors and tumor derived cell lines and to determine whether coexpression of c kit and stem cell factor could result in growth stimulation of breast tumor cells . ^^^ Expression of the c kit and stem cell factor genes in tissue specimens and cell lines was determined using an RNase protection assay , with confirmation of c kit protein expression by immunohistochemistry and Western blotting in tumor tissue and cell lines , respectively . ^^^ To determine the biological effects of coexpression of c kit and stem cell factor , the MCF 7 cell line , which expresses only stem cell factor , was transfected with a c kit expression vector . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Our results suggest that the differentiation pathway of basophils is different from that of mast cells , even though the receptor gene for stem cell factor ( c kit ) was expressed on the basophilic leukemic cells , as it was on mast cells . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We previously showed that the c kit messenger RNA is preferentially expressed in small cell lung cancer and that its ligand , stem cell factor , is expressed in a broad spectrum of human cancers . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor or steel factor ( SF ) is the ligand for the c kit receptor , and when combined with other hematopoietic growth factors , SF synergistically promotes the proliferation and differentiation of bone marrow stem cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Modulation of expression of the c kit proto oncogene product , the receptor for the recently identified stem cell factor , was studied on 12 O tetradecanoylphorbol 13 acetate ( TPA ) stimulated cultures of CD34+ normal bone marrow progenitor cells , blast cells from patients with primary acute myelogenous leukemia , cells from the leukemia cell lines HEL and MO7E , as well as cultured HMC 1 mast cells . ^^^ Treatment of virtually all cell types with nontoxic concentrations of TPA ( 10 ( 9 ) M ) for at least 48 h was associated with down regulation of synthesis of c kit transcripts and stem cell factor receptor surface expression . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The mRNAs encoding the c kit protooncogene tyrosine kinase receptor and its ligand , hemopoietic stem cell factor , are coexpressed in the majority of small cell lung cancer cell lines , suggesting that an autocrine growth loop may exist . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Furthermore , soluble c kit ligand / rh stem cell factor ( rhSCF ) , in vitro in combination with rhIL 3 and rhGM CSF or PIXY 321 , increased the size but not the number of colonies derived from recovery phase BFU Meg and CFU Meg . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We have screened genomic DNA from bone marrow mononuclear cells of 46 patients with myelodysplasia ( MDS ) for mutations / deletions of exons 6 , 13 , 17 , and 21 of the KIT gene ( stem cell factor receptor ) using polymerase chain reaction ( PCR ) , polyacrylamide gel electrophoresis , and autoradiography to detect single stranded conformational polymorphisms ( SSCP ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Human mast cells constitutively express the adhesion molecule ICAM 1 ( CD 54 ) and the receptor for stem cell factor ( Kit ) ( CD 117 ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
AG 1296 potently inhibits signaling of human PDGF alpha and beta receptors as well as of the related stem cell factor receptor ( c Kit ) but has no effect on autophosphorylation of the vascular endothelial growth factor receptor KDR or on DNA synthesis induced by vascular endothelial growth factor in porcine aortic endothelial cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The most important cytokine for the T cell dependent mechanism appears to be interleukin 3 , whereas for the fibroblast dependent mechanism it appears to be the ligand for the c kit receptor ( ie , stem cell factor ) . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Human foetal liver cells are an enriched source of mast cell progenitors that complete their differentiation and mature in response to stem cell factor , the ligand for Kit , in liquid culture . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
A high expression of Kit , the receptor for stem cell factor , was detected . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Characterization of these cells revealed several features distinct to each subset , in particular the expression of high levels of c kit ( the receptor for stem cell factor ) by CD44+CD25 TN and CD44+CD25+TN but not by CD 44 CD25+TN and CD 44 CD25 TN . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
KITG 559 and KITV 814 expressed in IC 2 cells were constitutively phosphorylated on tyrosine and demonstrated kinase activity in the absence of stem cell factor , which is a ligand for KIT . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The c kit proto oncogene product and its ligand stem cell factor play an important role in haematopoiesis , spermatogenesis , and melanogenesis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Coexpression of stem cell factor and c kit in embryonic and adult liver . ^^^ Stem cell factor and its receptor c kit constitute an important signal transduction system implicated in survival , proliferation , and differentiation of stem cells in hematopoiesis , gametogenesis , and melanogenesis . ^^^ In the adult liver both bile ducts and bile ductules were positive for stem cell factor and c kit . ^^^ When the activation of the liver stem cell compartment was induced by combining administration of acetylaminofluorene and partial hepatectomy , both stem cell factor and c kit were expressed in the infiltrating oval cell population , but absent in the newly formed basophilic hepatocytes . ^^^ Our data suggest that the stem cell factor / c kit signal transduction system is involved in the development of bile ducts and that it may also be an important member of the growth factor / receptor systems associated with the biology of liver stem cells . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor receptor ( c kit , CD 117 ) is expressed on blast cells from most immature types of acute myeloid mallignancies but is also a characteristic of a subset of acute promyelocytic leukaemia . ^^^ Investigating 208 patients with acute haematological malignancies , we found that stem cell factor receptor ( SCFR ) was expressed on high numbers of blast cells from the vast majority of patients ( 93 % ) with refractory anaemia with excess of blasts in transformation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We took advantage of the monoclonal antibody ( mAb ) 191B8 that was obtained by immunizing mice with cultured human thymocytes characterized by an immature surface phenotype [ CD 2 CD 3 CD 4 CD 8 stem cell factor receptor ( SCFR ) + ] and expressing cytoplasmic CD 3 epsilon chain . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The GrapSH 2 domain interacts with ligand activated receptors for stem cell factor ( c kit ) and erythropoietin ( EpoR ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
A critical role for stem cell factor and c kit in host protective immunity to an intestinal helminth . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
ICC may produce nitric oxide and amplify inhibitory neurotransmission . ( 5 ) Some classes of ICC fall to develop in animals with mutations in c kit or stem cell factor , the ligand for c Kit receptors . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The c kit protein is a transmembrane tyrosine kinase receptor that binds the growth factor stem cell factor . ^^^ Mutant alleles of the genes coding for both the receptor ( c kit ) and its ligand ( stem cell factor ) affect gametogenesis , development of melanoblasts and some aspects of haematopoiesis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The events primarily responsible for mast cell proliferation in mastocytosis are largely unknown , but a derangement of the network involving c kit receptor and its natural ligand ( stem cell factor , which promotes mast cell growth and differentiation in man ) is likely to have a primary role in this disease . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
By contrast , CP are present in germ free mice and in athymic ( nu / nu ) , SCID , TCR beta 10 delta / , RAG 2 / , PP deficient ( aly / aly ) , stem cell factor ( Sl / Sld ) and c kit ( W / Wv ) mutant mice . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor is a growth factor for normal human melanocytes , that acts through the tyrosine kinase receptor c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
However , the more restricted cellular distribution of the FLT 3 receptor may target the effects of FLT 3 ligand to primitive hematopoietic cells and to myeloid and lymphoid progenitor cells , in contrast to the pleiotropic effects of the c kit receptor ligand , stem cell factor . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
These cells expressed the NK markers asialoGM 1 , 10A7 , 2B4 , and Fc gammaRII / III but differed from FL A and splenic NK cells in expressing IL 2R alpha and stem cell factor receptor ( SCFR ) but no B 220 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
METHODS : Antibodies against c Kit ( e . g . , the receptor for stem cell factor ) were used to label ICs of the small intestines of embryos and neonatal mice . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We also found that EGCG inhibited the modulation of c kit , a receptor for stem cell factor , on leukemic cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In contrast to IL 7R alpha , loss of the stem cell factor receptor c kit was associated with enhanced lymphoid clonogenic potential and increased B lineage differentiation potential . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Action of human interleukin 4 and stem cell factor on erythroid and mixed colony formation by peripheral blood derived CD34+ c kit ( high ) or CD34+ c kit ( low ) cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The CD 117 ligand is stem cell factor , an important hematopoietic regulator . ^^^ Although CD 117 is a receptor for stem cell factor , its expression does not appear to correlate with CD 34 positivity . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Human megakaryoblastic leukemia cell lines have common biological features : high expression of the megakaryocytic specific antigen ( CD 41 ) ; high expression of early myeloid antigens ( CD 34 , CD 33 and CD 13 ) ; constitutive expression of IL 6 and platelet derived growth factor ; a complex karyotype picture ; expression of c kit ( the stem cell factor receptor ) ; growth dependency or stimulation by IL 3 and / or GM CSF ; and in vivo tumorigenicity in mice associated with marked fibrosis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We used CD 117 , the receptor for the stem cell factor ( also KIT oncogene ) as a new cellular surface marker . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In addition , c kit positive cases of SCLC showed autophosphorylation in response to recombinant human stem cell factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor , the ligand for the receptor encoded by c kit , is a major growth factor for mast cells in both rodent and primate species , and can modulate mast cell secretory function . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Cells within this population lacked markers of mature T cells but expressed heat stable antigen , the c kit receptor for stem cell factor , and / or the pre T cell alpha gene . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Induction of c kit molecules on human CD34+ / c kit < low cells : evidence for CD34+ / c kit < low cells as primitive hematopoietic stem cells . c kit , a receptor for stem cell factor , has been widely accepted as a distinctive marker for hematopoietic stem cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Expression of the stem cell factor receptor C KIT ( CD 117 ) in acute leukemias . ^^^ BACKGROUND AND OBJECTIVE : The receptor for stem cell factor ( CD 117 ) is the gene product of the c kit proto ontogene . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
CFU O was enriched in a nonadherent , low density , lineage marker negative ( Lin ) , Thy1 . 2 negative ( Thy1 . 2 ) , Sca 1 negative ( Sca 1 ) , and c kit positive ( c kit+ ) population , as were the progenitors that were responsive to macrophage colony stimulating factor ( CSF ; CFU M ) , granulocyte macrophage CSF ( CFU GM ) , and stem cell factor ( CFU SCF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor concentrations were measured using a commercially available ELISA kit according to the manufacturer ' s specifications . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
CD 14 and CD 122 , the interleukin 2 receptor beta chain ) and demonstrated a high degree of immaturity , as reflected by a high incidence of blasts expressing the stem cell factor receptor , CD 117 , and few blasts positive for the myeloid differentiation antigen CD 15 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Retroviral vector targeting human cells via c Kit stem cell factor interaction . ^^^ The ecotropic ( Moloney murine leukemia virus ) envelope protein was modified by insertion of a sequence encoding the N terminal 161 amino acids of murine stem cell factor ( mSCF ) , the ligand for murine c Kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We have also shown that anti TGF beta 1 can up modulate c KIT , the receptor of the stem cell factor ( steel factor ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The properties of MDS CD34+ cells are described here in association with proliferation and differentiation , with special emphasis on the role of stem cell factor ( a ligand for c kit ) in leukemic type growth of MDS CD34+ cells . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
BACKGROUND : The murine coat colour mutation recessive spotting ( rs ) maps very closely to the W / Kit locus , encoding the proto oncoprotein Kit , the protein tyrosine kinase receptor for stem cell factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In normal hemopoietic cells that are dependent on specific growth factors for cell survival , the expression of the basic helix loop helix transcription factor SCL / Tal1 correlates with that of c Kit , the receptor for Steel factor ( SF ) or stem cell factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
A synergistic interaction between EpoR and c kit is essential for the production of normal numbers of red blood cells , as demonstrated by the severe anemia of mice mutant for either c kit or its ligand , stem cell factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
When the purified cells were cultured in liquid phase for 10 days in the presence of recombinant human stem cell factor ( rSCF : a ligand for c kit ) , interleukin 3 ( rIL 3 ) , and thrombopoietin ( rTPO : a ligand for Mpl ) , the number of CD34+ / CD41+ cells increased to 19 % + / 7 % of total expanded cells on day 4 ( 4 days of liquid culture ) and then gradually decreased to 2 . 2 % + / 0 . 6 % on day 10 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
OBJECTIVE : To examine the expression of the c kit receptor and its ligand , stem cell factor , and their possible relation with apoptosis in infertile men . ^^^ MAIN OUTCOME MEASURE ( S ) : Expression of the c kit receptor protein , stem cell factor , and apoptosis in the testes . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Gab 1 is phosphorylated on tyrosine upon stimulation through the thrombopoietin receptor ( TPOR ) , stem cell factor receptor ( SCFR ) , and T cell and B cell antigen receptors ( TCR and BCR , respectively ) , in addition to IL 3R and gp 130 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Further , blocking the stem cell factor receptor , c kit , to facilitate complete ablation of mast cells over the period of parasite expulsion in resistant mice had no effect on the development of protective immunity . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Here we describe a second , distinct type of erythroid progenitor ( EpoR progenitors ) which can be established from freshly isolated bone marrow and is induced to self renew by ligands relevant for erythropoiesis , i . e . erythropoietin , stem cell factor , the ligand for c Kit and the glucocorticoid receptor ligand dexamethasone . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We found that anti c kit Abs mimicked the effect of stem cell factor and induced thymidine incorporation in Fc gamma RIIB / , but not in wild type ( wt ) mast cells unless Fc gamma RIIB were blocked or anti c kit F ( ab ' ) 2 were used . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
KIT receptor kinase activity is repressed , prior to stem cell factor binding , by unknown structural constraints . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The myeloid associated antigen CD 33 and the stem cell factor receptor ( CD 117 , c kit ) was expressed by 82 . 3 % and 73 . 5 % on CP CML patients and by 57 % and 57 . 5 % on healthy donors , respectively ( P < 0 . 005 ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
PU . 1 ( / ) HPC are unable to respond synergistically to erythropoietin plus stem cell factor and have reduced expression of c kit , which may explain the erythroid defect . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Inhibition of Kit expression by IL 4 and IL 10 resulted in a loss of Kit mediated signaling , as evidenced by reduced IL 13 and TNF alpha mRNA induction after stem cell factor stimulation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In addition , the cell line expressed the anti human epithelial related antigen ( MOC 31 ) , the human epithelial antigen ( HEA ) , and the gamma glutamyl transpeptidase , the hematopoietic growth factor , stem cell factor , and also its receptor , c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To further characterize cells within the CD 34 ( + ) / c kit ( + ) population that yield mast cells , this progenitor was additionally sorted for CD 13 , a myeloid marker known to appear early on rodent mast cells and cultured human mast cells , but not expressed or expressed at low levels on human tissue mast cells ; and cultured in recombinant human ( rh ) stem cell factor ( rhSCF ) , rh interleukin 3 ( rhIL 3 ; first week only ) , and rhIL 6 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Because several interleukin 3 independent mast cell lines with active mutations in the c kit gene did not release MMP 9 , the possible involvement of c kit receptor activation in downregulation of MMP 9 production was predicted . c kit receptor activation by stem cell factor led to a significant decrease in MMP 9 production of cultured mast cells and IC 2 mast cells transfected with the c kit gene . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Among various cytokines measured , including soluble c kit and stem cell factor , only the level of nerve growth factor was elevated . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Manipulating Kit signalling with reagents to block the receptor or downstream signalling pathways or by using mutant mice in which Kit or its ligand , stem cell factor , are defective has allowed novel studies into the specific functions of the different classes of ICC in several regions of the GI tract . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In wild type D 064 cells the accumulation of the cyclin dependent kinase inhibitor ( cdki ) p27kip 1 induces differentiation , which is dependent upon signalling via the ligand for the tyrosine kinase receptor c kit ( stem cell factor ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Expression of the receptor for stem cell factor , Kit protein , was examined on mast cells under conditions in which the dermal mast cell prevalence varied . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor and c kit gene expression and protein localization in the sheep ovary during fetal development . ^^^ The aim of this study was to investigate stem cell factor and c kit gene expression and protein localization in the mesonephros and ovary of sheep fetuses at different days of gestation , using RNA in situ hybridization and immunohistochemical procedures . ^^^ In conclusion , during follicle formation , c kit mRNA is localized to germ cells whereas c kit , together with stem cell factor protein , is localized to both germ cells and somatic cells , consistent with the hypothesis that the presence of this receptor ligand pair is essential to prevent apoptosis . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In order to explore a possible involvement of mast cells during human wound healing , we studied sections from scars ( 4 369 d old ) ( N = 20 ) and normal skin ( N = 10 ) for mast cell specific tryptase and chymase by enzyme histochemistry , for the stem cell factor receptor c Kit and the melanosomal marker TA 99 by immunohistochemistry , and for simultaneous c Kit expression and avidin fluorescence by double staining . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mastocytosis is a neoplastic disease caused at least in part by somatic mutations of the c KIT proto oncogene resulting in constitutive activation of its protein product , KIT , the receptor tyrosine kinase for stem cell factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Although it has been reported previously that pluripotent hemopoietic stem cells ( P HSCs ) express c kit , the receptor for stem cell factor ( steel factor ) , we and other groups have recently shown that P HSCs do not express c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In sheep , cows and pigs , numerous growth factor , growth factor receptor and gonadotrophin receptor mRNAs and peptides ( for example c kit , stem cell factor , GDF 9 , beta B and beta A activin / inhibin subunit , alpha inhibin subunit , follistatin , FGF 2 , EGF , EGF R , TGF beta 1 , 2 and 3 FSH R and LH R ) are expressed in a phase of growth ( for example types 1 5 ) specific and cell specific manner . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Canine mast cell tumors express stem cell factor receptor . c kit protooncogene encodes a type 3 transmembrane receptor kinase , the stem cell factor receptor , or KIT . ^^^ The ligand of the KIT . stem cell factor , is a cytokine that stimulates mast cell growth and differentiation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The total and proliferating cell nuclear antigen positive hepatic oval cells were significantly elevated in HGF transferred rats , in which stem cell factor and c kit mRNA increased at each time point . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To determine whether cytokine induced proliferation also limits the rate of short term engraftment and potential clinical utility of ex vivo expanded hematopoietic cells , murine Sca 1 ( + ) c kit ( + ) Lin ( ) cells were cultured in interleukin 6 ( IL 6 ) , IL 11 , granulocyte colony stimulating factor ( G CSF ) , stem cell factor , flk 2 ligand , and thrombopoietin for 7 days . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Twenty three cases with sinonasal natural killer / T cell lymphomas were collected from two high incidence regions : Beijing , China ( 14 cases ) and Osaka , Japan ( 9 cases ) . c kit mutations were analyzed on paraffin embedded specimens by PCR single strand conformation polymorphism followed by direct sequencing ; the c kit proto oncogene encodes a receptor of tyrosine kinase , which plays an important role in the regulation of normal and neoplastic hematopoiesis by the interaction with its specific ligand , termed stem cell factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
C kit proto oncogene product ( KIT , CD 117 ) is a tyrosine kinase growth factor receptor for stem cell factor . ^^^ C kit proto oncogene product ( KIT , CD 117 ) is a tyrosine kinase growth factor receptor for stem cell factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The staurosporine derivative PKC 412 was originally identified as an inhibitor of protein kinase C ( PKC ) and subsequently shown to inhibit other kinases including the kinase insert domain receptor ( KDR ) ( vascular endothelial growth factor receptor , VEGF R 2 ) , the receptor of platelet derived growth factor , and the receptor for the stem cell factor , c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Small cell lung cancer ( SCLC ) is an aggressive cancer characterized by several autocrine growth mechanisms including stem cell factor and its receptor c Kit . ^^^ A downstream phosphorylation target of c Kit , Akt , was not phosphorylated in response to stem cell factor in the presence of STI 571 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Soluble stem cell factor receptor ( CD 117 ) and IL 2 receptor alpha chain ( CD 25 ) levels in the plasma of patients with mastocytosis : relationships to disease severity and bone marrow pathology . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Coupling of the Kit receptor protein on gonocytes to stem cell factor from Sertoli cells is vital for successful migration by gonocytes , as antagonism of the former suppresses and addition of the latter stimulates gonocyte migration . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
However , this was not due to an intrinsic inability to form ruffles and lamellipodia because both were restored by stimulation of cells with phorbol 12 myristate 13 acetate in a Rac dependent manner , and by stem cell factor in melanoblasts expressing the receptor tyrosine kinase c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
By means of direct dephosphorylation , it down regulates a broad spectrum of growth promoting receptors , including the Kit tyrosine kinase , activated to elicit a prominent cascade of intracellular events by stem cell factor binding . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The transcription factor paired box 3 ( PAX 3 ) works at the promoter region of the microphthalmia associated transcription factor ( MITF ) gene , and the MITF transcription factor orders the expression of c kit , which encodes the receptor for stem cell factor , which in turn stimulates melanoblast migration from the neural tube to the skin in the embryo . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mutations of the c kit proto oncogene ( stem cell factor receptor ) and the p 53 tumor suppressor gene were examined in the MDS lesion and malignant lymphoma ( ML ) by the polymerase chain reaction single strand conformational polymorphism ( PCR SSCP ) method followed by direct sequencing . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The granulocyte macrophage colony formation of CD 34 ( + ) / Kit ( + ) / G CSFR ( + ) cells in patients was markedly decreased in response to G CSF alone and to the combination of stem cell factor , the ligand for flk2 / flt3 , and IL 3 with or without G CSF in serum deprived semisolid culture . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Immunohistological distribution of stem cell factor and kit receptor in angiosarcoma . ^^^ These results suggest that tumor cell derived stem cell factor may play a role in the increased number of mast cells , via the kit receptor , which may contribute to the proliferation of tumor cells , leading to the progression of angiosarcoma . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We used as experimental models wild type mast cells , which constitutively express the stem cell factor receptor Kit and FcgammaRIIB , FcgammaRIIB deficient mast cells reconstituted with wild type or mutated FcgammaRIIB , and Src homology 2 domain containing inositol polyphosphate 5 phosphatase 1 ( SHIP 1 ) deficient mast cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Previously we have demonstrated that stem cell factor and its receptor c kit are expressed in neural crest derived tumor cells and that a c kit block induces their apoptosis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Manipulating Kit signaling with reagents to block the receptor or downstream signaling pathways or by using mutant mice in which Kit or its ligand , stem cell factor , are defective has allowed novel studies of the development of these cells within the tunica muscularis and also allowed the study of specific functions of different classes of ICC in several regions of the GI tract . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The expression levels of stem cell factor , interleukin ( IL ) 7 , and IL 15 in IEC , and their corresponding receptors , 1 . e . c kit , IL 7 receptor , and IL 15 receptor , in CD8alphaalpha+ IEL were reduced following oral feeding with LT . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Gastrointestinal stromal tumor ( GIST ) cells overexpress the stem cell factor receptor CD 117 , the product of the proto oncogene c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mast cell progenitors ( c kit ( + ) CD 13 ( + ) cells with chloroacetate esterase activity ) were purified from 4 week old cultures of cord blood mononuclear cells maintained in stem cell factor , interleukin 6 ( IL 6 ) , and IL 10 using a CD 14 depletion column . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Genes and cancers : c Kit , SCFR ( stem cell factor receptor ) CD 117 ] . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Testosterone repressed prostate message 2 ( TRPM 2 ) was upregulated , while c kit ( stem cell factor receptor ) mRNA was downregulated following DBP exposure . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Antibodies were used to vimentin , CD 31 , CD 34 , alpha smooth muscle actin , S 100 protein , thrombomodulin , calretinin and c kit ( CD 117 ) , a tyrosine kinase growth factor receptor for stem cell factor usually expressed by embryonic / fetal endothelium . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Recent studies suggest that activating mutations of c kit , a protooncogene encoding for the receptor ( kit ) of stem cell factor , are a possible cause of some forms of mastocytosis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
SU 5416 is a competitive inhibitor of VEGF receptor subtypes VEGFR 1 and VEGFR 2 and stem cell factor receptor c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Ovarian development and function have been extensively studied in eutherian species , with stem cell factor and its receptor , c kit , having been shown to play key roles at various stages of these processes . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Antibody against stem cell factor receptor , c kit , had higher sensitivity and specificity than pi GST and CK 19 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The interaction between stem cell factor and its receptor , c Kit , has been found to be essential for the maturation of male germ cells and peripheral mast cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Transforming growth factor beta 1 regulates melanocyte proliferation and differentiation in mouse neural crest cells via stem cell factor / KIT signaling . ^^^ Stem cell factor is essential to the migration and differentiation of melanocytes during embryogenesis based on the observation that mutations in either the stem cell factor gene , or its ligand , KIT , result in defects in coat pigmentation in mice . ^^^ These results indicate that transforming growth factor beta 1 affect melanocyte precursor proliferation and differentiation in the presence of stem cell factor / KIT in an autocrine / paracrine manner . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Although no recurrent cytogenetic abnormalities specific for mastocytosis have yet been recognized , the majority of cases display one of several mutations in c KIT , the gene encoding the receptor for stem cell factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In spite of similarities in chromosomal location , down regulation upon differentiation of TGCTs , and PDGF alpha receptor and c KIT ( the stem cell factor receptor ) both being a tyrosine kinase receptor , no correlation was found between activity of the P 2 promoter of the PDGF alpha receptor gene and expression of c KIT . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
A strong c kit and stem cell factor expression was detected in the Hodgkin and Reed Sternberg cell line L 1236 , but not in 20 primary cases of classical Hodgkin ' s disease . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Immunohistochemical analysis was performed on paraffin embedded tissue using monoclonal antibodies against Ki 67 and thyroid transcription factor 1 ( TTF 1 ) and polyclonal antibodies against c Kit antigen ( a stem cell factor receptor / mast cell growth factor receptor ) in conjunction with Leder stain for naphthol ASD chloroacetate esterase . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Several models with defects in ICC networks have been developed , and these include animals with defects in the Kit signaling pathway ( e . g . , white spotting mutants that have defects in Kit receptors ; steel mutants that have mutations in stem cell factor , the ligand for Kit ; and animals that are chronically treated with reagents that block Kit or downstream signaling proteins ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
All 3 indolinones inhibited phosphorylation of WT Kit in the presence of stem cell factor at concentrations as low as 0 . 01 microM . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
This review focuses on selected areas implicated in male infertility including : ( 1 ) autosomal and sex chromosomal abnormalities ; ( 2 ) genetic disorders associated with impaired gonadotrophin secretion or action ; ( 3 ) microdeletions within regions of the Y chromosome containing candidate gene families for spermatogenesis ; ( 4 ) the genetic nexus between cystic fibrosis and congenital bilateral absence of the vas deferens ; and ( 5 ) insights into human infertility as gleaned from animal studies into mechanisms involving the Bcl 2 family of apoptosis regulators and the interaction between the c kit encoded tyrosine kinase receptor and its ligand , stem cell factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
CD 45 ( ) Sca 1 ( + ) c kit ( ) cells expanded ex vivo in 5 ng / mL murine stem cell factor , mFlt 3L , and megakaryocyte growth and development factor ( MGDF ) for 9 days increased their in vivo hematopoietic repopulating potential 5 . 3 fold relative to fresh cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
GISTs characteristically express the KIT protein , a transmembrane tyrosine kinase receptor for stem cell factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Here we demonstrate , by use of immobilized synthetic phosphopeptides corresponding to various autophosphorylated tyrosine residues in the receptor for stem cell factor ( c Kit ) , that APS preferentially associates with phosphorylated Tyr 568 and Tyr 936 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stimulation of Kit by its ligand , stem cell factor activates several distinct early signaling pathways , including phospholipase C gamma , phosphatidylinositol 3 kinase , Src kinase , Grb 2 , and Grb 7 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Sequencing of c kit complementary DNA from human GIST cells has demonstrated a high frequency of mutations that lead to constitutive activation of the KIT tyrosine kinase in the absence of stimulation by its physiologic ligand ( stem cell factor ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
PURPOSE : The tyrosine kinase receptor c kit and its ligand stem cell factor are coexpressed in many small cell lung cancer ( SCLC ) cell lines , leading to the hypothesis that this coexpression constitutes an autocrine growth loop . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor plays a key role in the development of human mast cells via interaction with Kit receptor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Besides the Bcr Abl protein , the drug is also active against other tyrosine kinases , such as Abl , the stem cell factor receptor ( c kit ) and the platelet derived growth factor receptor , whose inhibition might have potential implications for the treatment of several malignancies . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In mice , expression of the receptor for stem cell factor , c kit , on dermal mast cells correlates with prevalence . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Increment of murine spermatogonial cell number by gonadotropin releasing hormone analogue is independent of stem cell factor c kit signal . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
GISTs are now defined by their overexpression of CD 117 ( KIT ) , the receptor for the stem cell factor , and can thus be discriminated from smooth muscle tumors . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
It also inhibits phosphorylation of Kit ( stem cell factor receptor ) and platelet derived growth factor receptor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In biochemical assays , SU 10944 exhibits potent inhibitory activity against VEGFR 1 ; weak activity against other related subgroup members , including stem cell factor receptor ( SCFR ) , platelet derived growth factor receptor beta ( PDGFRbeta ) , and fibroblast growth factor receptor 1 ( FGFR 1 ) ; and no detectable activity against other protein tyrosine kinases such as epidermal growth factor receptor ( EGFR ) , Src , and hepatocyte growth factor receptor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
As a result of the latter event , Kit negative spermatogonia acquire sensitivity to Stem Cell Factor . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
METHODS : Liver tissues taken from 59 SH patients were tested for the receptor of stem cell factor ( c kit ) , pi class glutathione S transferase ( GST pi ) , cluster of differentiation 34 ( CD 34 ) , cytokeratin 19 ( CK 19 ) , cytokeratin 18 ( CK 18 ) and alpha fetoprotein ( AFP ) by immunohistochemistry ( IHC ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The mutant c Kit proteins , which are activated without a stem cell factor , contribute to the tumor development . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
C kit and its ligand stem cell factor regulate melanocyte development and survival . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mast cell proliferation , differentiation , survival and activation are regulated by stem cell factor , the ligand for the c kit tyrosine kinase receptor , expressed on the mast cell surface . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Both withdrawal of stem cell factor from our expansion cultures or functional inhibition of c kit led to a similar degree of inhibition of expansion , whereas the effect of imatinib was substantially greater at all dose levels tested . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Inhibition of the c abl , platelet derived growth factor receptor and stem cell factor receptor ( c kit ) tyrosine kinases by imatinib has also been reported . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In the prehypertensive ( 2 week old ) SHRs , the increased expression of c kit and soluble stem cell factor correlated with an increased number of cardiac mast cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
One of the undoubted major breakthroughs in the recent treatment of cancer is imatinib , a tyrosine kinase inhibitor of the bcr abl fusion protein , the stem cell factor receptor c kit ( KIT ) and the platelet derived growth factor receptor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Definite melanosomes on electron microscopic analysis and coexpression of HMB 45 antigen and stem cell factor receptor ( CD 117 ) may provide the clue to understanding perivascular epithelioid cell tumor because angiomyolipoma also coexpresses HMB 45 antigen and CD117 . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Reverse transcription polymerase chain reaction of RNA isolated from human melanocytes exposed to SPC revealed an upregulation of mRNA transcripts for tyrosinase , microphthalmia associated transcription factor M , endothelin B receptor and the stem cell factor receptor , c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
MM cells expressed abl , but its level of tyrosine phosphorylation was low and unaffected by treatment with STI 571 . c Kit was also expressed in certain MM cell lines , and its phosphorylation was stimulated by stem cell factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor receptor ( c KIT ) codon 816 mutations predict development of bilateral testicular germ cell tumors . ^^^ ITGCN cells are characterized , among others , by the presence of stem cell factor receptor c KIT . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The product of the proto oncogene c kit is a transmembrane receptor protein that plays an important role in the regulation of normal and neoplastic hematopoiesis via the interaction with its specific ligand termed stem cell factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
RESULTS : Although long term engraftment was predominantly detected in Flt 3 ( ) populations as previously described , a 6 day cultivation of Lin ( / low ) c kit ( + ) Sca 1 ( + ) Flt 3 ( ) bone marrow cells with stem cell factor and interleukin 11 resulted in the generation of Flt 3 ( + ) HSCs with long term engraftment capabilities . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To investigate the relationship between c kit expression and cell cycle regulation by endogenous transforming growth factor beta ( TGF beta ) in human bone marrow hematopoietic progenitor cells , CD34+ CD 38 c kit ( low / ) and CD34+ CD 38 c kit ( high ) populations were cultured in stem cell factor , thrombopoietin , interleukin 3 ( IL 3 ) , IL 6 , granulocyte colony stimulating factor , granulocyte / macrophage colony stimulating factor and anti TGF beta , and analyzed for cell cycle status . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mutations of the KIT gene are known to be present in most gastrointestinal stromal tumors and result in gain of function , with permanent activation of the expressed KIT receptor in the absence of binding of the stem cell factor ligand . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We present a case of a BPB with emphasis on expression of the stem cell factor receptor KIT ( CD 117 ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Lineage marker depleted ( Lin ( ) ) murine bone marrow cells expressing stem cell antigen 1 ( Sca 1 ) were sorted on the basis of stem cell factor receptor ( c kit ) expression to obtain Lin ( ) Sca ( + ) Kit ( + ) or Lin ( ) Sca ( + ) Kit ( ) cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To evaluate the etiologic role of ultraviolet ( UV ) radiation in acquired dermal melanocytosis ( ADM ) , we investigated the effects of UVA and UVB irradiation on the development and differentiation of melanocytes in primary cultures of mouse neural crest cells ( NCC ) by counting the numbers of cells positive for KIT ( the receptor for stem cell factor ) and for the L 3 , 4 dihydroxyphenylalanine ( DOPA ) oxidase reaction . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In contrast , Erk activation induced by stem cell factor , which activates c Kit in the same cells , is sensitive to Raf inhibition and insensitive to PI3K and PKC inhibitors . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Imatinib mesylate is a small molecule drug that in vitro inhibits the Abelson ( Abl ) , Arg ( abl related gene ) , stem cell factor receptor ( Kit ) , and platelet derived growth factor receptor A and B ( PDGFRA and PDGFRB ) tyrosine kinases . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The overwhelming majority of GISTs express the KIT protein , the transmembrane receptor tyrosine kinase for the stem cell factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
BACKGROUND : CD 34 ( + ) progenitor cells develop into tryptase ( + ) , CD 117 ( + ) mast cells when cultured in the presence of recombinant human stem cell factor ( rhSCF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Differentially expressed genes like the stem cell factor receptor c kit and the chemokine receptor CCR 5 can contribute to the homing and differentiation of hepatic NK cells in the liver sinusoids . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
BACKGROUND : Mutations of the stem cell factor receptor C KIT play a major pathogenetic role in the development of different malignant diseases like human mastocytosis , myeloproliferative disorders , gastrointestinal stromal tumors , acute myelogenous leukemia , and sinonasal lymphomas . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Germ cells were characterized by expression of stem cell factor receptor c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
AIM : To investigate the expression of CD 34 and c kit ( receptor of stem cell factor ) in cholangiocarcinoma . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Signal transduction via the stem cell factor receptor / c Kit . ^^^ Together with its ligand , stem cell factor , the receptor tyrosine kinase c Kit is a key controlling receptor for a number of cell types , including hematopoietic stem cells , mast cells , melanocytes and germ cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Germ cells were localized by expression of a specific antibody for stem cell factor receptor c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
RESULTS : In all cases , we showed the presence of wild type KIT receptors by analyzing the entire coding sequence , which together with the detection of the cognate ligand stem cell factor , supports the establishment of an autocrine loop . ^^^ CONCLUSIONS : The KIT molecular profile derived from the analysis of SCLC surgical specimens shows that wild type KIT is overexpressed and phosphorylated in the presence of stem cell factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The tyrosine kinase receptor c kit ( CD 117 ) and its ligand stem cell factor are considered to be co expressed in various solid tumors , including adenocarcinomas of the lung . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
BACKGROUND AND AIMS : The c kit protooncogene receptor and its ligand stem cell factor regulate the proliferation and survival of germ cells as well as interstitial cells of Cajal ( ICCs ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
SU 11657 ( SUGEN ) is a selective multitargeted tyrosine kinase inhibitor with antitumor and antiangiogenic activity exerted by targeting PDGF receptors ( PDGFR ) , VEGF receptors ( VEGFR ) , stem cell factor receptor ( c KIT ) , and FMS related tyrosine kinase 3 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
METHODS : Using immunohistochemistry , the following markers were studied : placental / germ like cell alkaline phosphatases ( PLAP ) , the marker of pluripotency OCT3 / 4 , the proliferation marker Ki 67 , beta catenin and E cadherin , the stem cell factor receptor c KIT , and VASA , a protein specific for the germ cell lineage . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Gastrointestinal stromal tumor ( GIST ) is the most common mesenchymal tumor in the digestive tract and the majority of GIST has characteristic gain of function mutations of the c kit gene , which encodes the KIT receptor for stem cell factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Thrombopoietin alone or in the presence of stem cell factor supports the growth of KIT ( CD 117 ) low / MPL ( CD 110 ) + human mast cells from hematopoietic progenitor cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Of 43 ( 35 % ) tumors tested for both c kit receptor and its ligand ( stem cell factor ) , 15 expressed both proteins indicating the possibility that this autocrine stimulation feedback loop is a factor in the growth of some ovarian cancers . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Herein , we will review the current state of the art using agents that target as prototypes Bcr Abl , platelet derived growth factor receptor ( PDGFR ) , KIT ( stem cell factor receptor ) , and epidermal growth factor receptor ( EGFR ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Furthermore , several membrane proteins were among the 48 decreased genes correlating most closely with the functional assays , including the stem cell factor receptor c Kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Imatinib also inhibits the c abl , platelet derived growth factor receptor ( PDGFR ) , abl related gene and stem cell factor receptor , c kit , protein tyrosine kinases . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Expression of c Kit ( CD 117 ) and stem cell factor / c Kit mediated cell proliferation were tested in vitro in young and old B6D2F1 mice to study the role of c Kit signaling in hematopoietic stem cell ( HSC ) senescence . ^^^ Sorted Lin ( ) Sca 1 ( + ) CD 117 ( + ) BM cells from an old mouse expanded 5 fold when cultured in vitro for 72 hours with stem cell factor at 25 ng / ml , which was significantly higher than a 2 . 5 fold expansion of the same cells from a young donor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The BUC layer also stained for the stem cell factor c Kit suggesting its involvement in urothelial cell development . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mechanisms leading to the activation and the proliferation of hepatic progenitor cells are still largely unknown : they involve growth factors as the stem cell factor , ligand of c kit , cytokines , chemokines as SDF 1 a and vagal or sympathetic innervatio . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Diagnosis of mastocytosis is based on appropriate stains ( Giemsa , toluidine blue ) and immunophenotype features ( tryptase , CD 117 , also known as c KIT and stem cell factor receptor ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Interventions : Interventions included hematoxylin eosin staining , immunohistochemistry for octamer binding transcription factor ( OCT ) 3 / 4 , gene encoding the stem cell factor receptor that has tyrosine kinase activity c KIT , placental / germ alkaline phosphatase ( PLAP ) , testis specific protein Y encoded ( TSPY ) , and VASA , double staining for OCT3 / 4 and VASA , with ploidy determination by fluorescent in situ hybridization . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The AML blast cells in these elderly patients are often characterised by several unfavourable covariates that predict the poor treatment outcome , including high stem cell marker CD 34 expression , minimally or undifferentiated features , high P glycoprotein expression , high bcl 2 / bax ratio , unfavourable karyotype and more frequent internal tandem duplications ( ITDs ) and mutations of class 3 receptor type tyrosine kinase for key haematopoietic cytokines : Flt 3 ( receptor for Flt ligand ) , c kit ( receptor for stem cell factor ) and fms ( receptor for M CSF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Signaling by Kit protein tyrosine kinase the stem cell factor receptor . ^^^ Signaling by stem cell factor and Kit , its receptor , plays important roles in gametogenesis , hematopoiesis , mast cell development and function , and melanogenesis . ^^^ Stem cell factor exists as both a soluble and a membrane bound glycoprotein while Kit is a receptor protein tyrosine kinase . ^^^ The complete absence of stem cell factor or Kit is lethal . ^^^ Binding of stem cell factor to Kit results in receptor dimerization and activation of protein kinase activity . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
MEP cells , but not PEM cells , express c kit , respond to stem cell factor in vitro , and form spleen colonies in vivo . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
These were positive for the stem cell factor receptor c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Two of these mutations in exon 17 were novel , and the other seven mutations were identical to the already known gain of function mutations which cause activation of KIT without ligand stem cell factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Structure and regulation of Kit protein tyrosine kinase the stem cell factor receptor . ^^^ Signaling by stem cell factor and Kit , its receptor , play important roles in gametogenesis , hematopoiesis , mast cell development and function , and melanogenesis . ^^^ Stem cell factor exists as both a soluble and a membrane bound glycoprotein while Kit is a glycoprotein receptor protein tyrosine kinase . ^^^ The complete absence of stem cell factor or Kit is lethal . ^^^ Binding of stem cell factor to Kit results in receptor dimerization and activation of protein kinase activity . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
W and Sl encode the Kit receptor tyrosine kinase and its ligand , stem cell factor , respectively . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
DISCUSSION : Imatinib mesylate is a protein tyrosine kinase inhibitor that inhibits the BCR ABL tyrosine kinase , the receptor tyrosine kinases for platelet derived growth factor , and stem cell factor c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Cells expressing the stem cell factor receptor , c kit , contribute to neoangiogenesis in diabetes . ^^^ Origins from bone marrow and endothelial cell differentiation were identified by immunolabelling for the stem cell factor receptor , c kit and von Willebrand factor ( vWF ) , respectively . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Ba / F3 cells expressing KIT ( WT ) , KIT ( L 540 ) and KIT ( K 563 ) showed dose dependent tyrosine phosphorylation after treatment with increasing concentrations of recombinant mouse stem cell factor ( rmSCF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Expression of intracellular oncogenic kinase BCR / Abl , myeloid cell surface antigen CD 33 , human stem cell factor receptor c Kit and integrin receptor VLA 4 were investigated using model human leukemia cell lines . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
BACKGROUND : Small cell lung carcinoma ( SCLC ) cell lines commonly express KIT and its ligand , stem cell factor , suggesting an autocrine loop promoting cell growth . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Furthermore , blast cells of the AML 1 mutated patients expressing surface c KIT , and SHP 2 mutants contributed to prolonged and enhanced extracellular signal regulated kinase activation following stem cell factor stimulation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The stem cell factor receptor / c Kit as a drug target in cancer . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The precise roles played by the transmembrane receptor tyrosine kinase c kit and its ligand stem cell factor in early T cell development are difficult to study . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Germ cell proliferation , migration and survival during all stages of spermatogenesis are affected by stem cell factor signalling through the c Kit receptor , the expression and function of which are vital for normal male reproductive function . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
METHODS : Seven PVS cases were studied with antibodies for smooth muscle actin ( SMA ) , muscle specific actin ( MSA ) , monoclonal desmin , S 100 protein , CD 31 , CD 34 , CD45RO , CD 68 , CD 99 , Ki 67 ( MIB 1 ) , and with antibodies directed against several receptor tyrosine kinases ( RTK ) , including platelet derived growth factor alpha and beta receptor ( PDGFR alpha and beta ) , epidermal growth factor receptor ( EGFR ) , fibroblast growth factor receptor ( FGFR ) , vascular endothelial growth factor 1 and 2 receptor ( VEGFR ) , and stem cell factor receptor ( c kit ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Finally , immunohistochemical detection of the stem cell factor receptor ( c Kit ) revealed its specific induction which coincided with nestin immunolabelling . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
However , because imatinib inhibits c kit , the stem cell factor receptor , it could interfere with bone marrow engraftment . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Proliferative activity ( proliferating cell nuclear antigen ( PCNA ) protein expression ) , hepatic growth factor ( HGF ) receptor ( c Met ) , stem cell factor receptor ( c kit ) , Notch 2 and Hes 1 expression were also evaluated . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Expression of c kit , a stem cell factor receptor , and nestin was examined in porcine Muller cells cultured with basic fibroblast growth factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stem cell factor is another important hematopoietic cytokine that signals through c Kit , a receptor tyrosine kinase , and regulates hematopoietic stem cell maintenance and erythroid development . ^^^ Furthermore , alphaGMR inhibited c Kit auto phosphorylation induced by the ligand stem cell factor . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
We hypothesized that hypomelanosis in hypopigmented mycosis fungoides may have a similar mechanism as in vitiligo , a condition in which it is believed that alterations in expression of CD 117 ( stem cell factor receptor / KIT protein ) on epidermal melanocytes and abnormal interactions between melanocytes and surrounding keratinocytes may play a pathogenic role . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Direct binding of Cbl to Tyr 568 and Tyr 936 of the stem cell factor receptor / c Kit is required for ligand induced ubiquitination , internalization and degradation . ^^^ The ubiquitin E 3 ligase Cbl has been shown to negatively regulate tyrosine kinase receptors , including the stem cell factor receptor / c Kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Receptor tyrosine kinases ( RTK ) , such as vascular endothelial growth factor receptor ( VEGFR ) , platelet derived growth factor receptor ( PDGFR ) , stem cell factor receptor ( KIT ) , and fms like tyrosine kinase 3 ( FLT 3 ) , are expressed in malignant tissues and act in concert , playing diverse and major roles in angiogenesis , tumor growth , and metastasis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
AIM : An oral , multitargeted receptor tyrosine kinase inhibitor , SU 11248 , inhibits vascular endothelial growth factor receptor 2 ( VEG FR 2 , kinase domain receptor F1K1 ) , platelet derived growth factor receptor , stem cell factor receptor ( Kit ) , and fetal liver tyrosine kinase 3 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Kit positive proliferating male primordial germ cells ( PGCs ) show low levels of telomerase activity , but they increase telomerase activity upon Kitl stimulation . ^^^ Diplotene arrested growing oocytes that reexpress Kit do not increase telomerase activity upon Kitl stimulation . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To investigate the mechanisms of the pigment pattern formation of plumage and clarify the roles of the Bh locus in the pattern formation , we examined the expression pattern of genes relating to melanocyte development ( Mitf , MelEM antigen , Kitl , Kit and EdnrB 2 ) and melanin pigment production ( Dct , Tyrp 1 , Tyr and Mmp 115 ) in Bh mutant and wild type embryos throughout development . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
JMML arises from clonal expansion of a hematopoietic stem cell , and JMML cells and murine Nf 1 / hematopoietic cells are hypersensitive to granulocyte macrophage colony stimulating factor and KitL , the ligand for c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Kit and its ligand , Kitl , function in hematopoiesis , melanogenesis , and gametogenesis . ^^^ In the testis , Kitl is expressed by Sertoli cells and Kit is expressed by spermatogonia and Leydig cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Genes expressed in the germ cells such as Figla , Nobox , Kit and Ntrk 2 , as well as genes expressed in the surrounding somatic cells such as Foxl 2 , Kitl and Ngf , play critical functions during early folliculogenesis . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
This stability of pigmented spots also applies to Kit ( W ) and Kitl ( Sl ) mutant mice . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The membrane anchored forms of transforming growth factor alpha ( TGF alpha ) and stem cell growth factors ( Kit ligands ) KL 1 and KL 2 are converted to soluble growth factor forms by a regulated proteolytic cleavage process . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
To evaluate the sensitivity of immunohistochemical techniques in diagnosing monophasic fibrous SS ( MFSS ) and poorly differentiated SS ( PDSS ) , we examined 60 t ( 10 ; 18 ) ( SYT SSX ) positive cases ( 47 MFSS and 13 PDSS ) for cytokeratin AE1 / AE3 , cytokeratin KL 1 , epithelial membrane antigen , E cadherin , CD 34 , S 100 protein , alpha smooth muscle actin , desmin , h caldesmon , CD 99 , bcl 2 , and C kit ( CD 117 ) antibodies . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Moreover , ADAM 19 cleaves peptides corresponding to the known cleavage sites of tumor necrosis factor alpha ( TNF alpha ) , TNF related activation induced cytokine ( TRANCE , also referred to as osteoprotegerin ligand ) , and kit ligand 1 ( KL 1 ) in vitro . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Stromal cells or epithelial cells derived from fetal thymus or liver , which can support growth and differentiation of c kit+ lymphocyte progenitor clones , synthesize mRNA for Steel Factor ( SF ) , the ligand of c kit . ^^^ Using Cos 1 cells transfected with mouse SF cDNA and an antagonistic c kit receptor specific antibody , we show that the c kit / SF system contributes to the survival of lymphocyte progenitors and enhances the proliferative responses of these cells to other growth factors ( i . e . ^^^ However , the c kit receptor / SF ligand pair is neither sufficient nor necessary for the differentiation of lymphocyte progenitors into mature T or B lymphocytes . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Expression of CSF 1 / c fms and SF / c kit mRNA during preimplantation mouse development . c fms and c kit are structurally related tyrosine kinase receptors for colony stimulating factor 1 ( CSF 1 ) and for steel factor ( SF ) , respectively . ^^^ The patterns of CSF 1 / c fms mRNA and SF / c kit mRNA expression are consistent with the hypothesis that these two ligand / receptor systems may act in a compensatory or synergistic manner during preimplantation development . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Entry into the cell cycle of dormant hematopoietic progenitors appears to be regulated by multiple synergistic factors , including interleukin 6 ( IL 6 ) , granulocyte colony stimulating factor ( G CSF ) , IL 11 , and the ligand for c kit , which is also known as steel factor ( SF ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Steel factor ( SF ) , the ligand for the proto oncogene c kit , acts synergistically with GM CSF or IL 3 to support the growth of normal human hematopoietic progenitor cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Mutations in the Steel locus , encoding a growth factor ( Steel factor or SF ) or c kit , the gene encoding its receptor , result in severe anemia in the mouse . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The recent simplification of the method of measurement of U citrate ( Boehringer Mannheim kit ) led us to address this issue in 118 male idiopathic ( 1 ) SF , with either idiopathic hypercalciuria of the renal ( n = 6 ) , absorptive ( n = 19 ) or dietary ( n = 41 ) type , or with normocalciuria ( n = 52 ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
This SF induced phosphorylation also occurred within 10 min of incubation at 4 degrees C , indicating that this might be a relatively early event in the c kit signaling pathway . ^^^ Last , this SF induced phosphorylation of the IL 3R occurred in the presence of the tyrosine kinase inhibitor , genistein , at levels which blocked the autophosphorylation of c kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
TGF beta 1 has been shown to repress human and murine leukemic cell and murine lin bone marrow mononuclear cell expression of the receptor for SF ( c kit ) . ^^^ We speculated that TGF beta 1 might exert its inhibitory effect on hematopoiesis in part by decreasing SF / c kit interactions . ^^^ Therefore , we tested the hypothesis that TGF beta 1 inhibits both stromal cell expression of SF and hematopoietic progenitor cell expression of c kit . ^^^ We measured stromal cell expression of SF protein and hematopoietic progenitor cell expression of membrane bound c kit before and after exposure to recombinant human TGF beta 1 . ^^^ Both stromal cell expression of SF protein and hematopoietic progenitor cell expression of c kit protein were inhibited 50 % to 80 % by TGF beta 1 . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In SF embryos the c kit expressing melanoblasts migrate initially in the dorso lateral migration pathway as in normal birds . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Steel factor ( SF ) , the ligand for c kit , is an essential regulator of normal hematopoiesis , melanogenesis , gametogenesis , and mast cell growth and development . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Optimal colony formation from c kit ( low ) cells from 5 FU treated mice required the interactions of at least two factors among interleukin 3 ( IL 3 ) , IL 11 and steel factor ( SF ) whereas colony formation from c kit ( high ) cells of normal mice was supported well by IL 3 alone . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Taken together , these findings are consistent with the view that SF activation of c kit receptor induced signaling events is not a rate limiting mechanism controlling red blood cell production during normal development until hematopoietic cells differentiate beyond the day 3 BFU E stage . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In the presence of varying combinations of TPO , Steel factor ( SF ) , and interleukin 3 ( IL 3 ) , CD34+ / c kit ( low ) / CD38neg / low cells produced fewer colonies than CD34+ / c kit ( low ) / CD38high cells . ^^^ In suspension culture of CD34+ / c kit ( low ) / CD38neg / low cells , TPO acted synergistically with SF and / or IL 3 in support of the production of colony forming cells for granulocyte / macrophage colonies , erythroid colonies , and mixed colonies . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In contrast , the receptor for SF , i . e . , Kit , is highly expressed on very immature CD 34 bright / HLA DR dull cells , which include stem cells . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The receptor for SF , c Kit , contains intrinsic tyrosine kinase activity , and binding of SF induces rapid tyrosine phosphorylation of several cellular proteins , including c Kit itself . ^^^ We conclude that SF induces the formation of a signaling complex potentially containing CRKL and p 120 ( CBL ) , both of which bind to c Kit through p 85 ( PI3K ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
TPO in combination with the ligand for c kit ( SF ) or interleukin 3 ( IL 3 ) but not TPO alone supported the growth of murine primitive hematopoietic progenitors . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Genetics studies have shown that the SI locus encodes the Steel factor ( SF ) , which is the ligand for the tyrosine kinase receptor c kit , the product of the W locus . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
This SF induced degranulation , which was not due to higher c kit levels in SHIP / cells , correlated with higher intracellular calcium than that in SHIP+ / + cells and was dependent on the influx of extracellular calcium . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
As a first step toward identifying the mechanisms involved in mediating such cytokine dose dependent effects , we have now investigated the kinetics of SF receptor ( c kit ) internalization by human Mo7e cells exposed to different extracellular concentrations of soluble SF . ^^^ Transfer of Mo7e cells to a higher concentration of SF caused an initially rapid downregulation of cell surface c kit which was accompanied by a rapid depletion of extracellular SF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
In patients with SCN , the nonadherent light density bone marrow cells and cells that were purified on the basis of the expression of CD 34 and Kit receptor ( CD 34 ( + ) / Kit ( + ) cells ) showed the reduced response to the combination of steel factor ( SF ) , the ligand for flk2 / flt3 ( FL ) , and interleukin 3 ( IL 3 ) with or without G CSF in serum deprived culture . ^^^ Furthermore , when individual CD 34 ( + ) / Kit ( + ) cells from patients were cultured in the presence of SF , FL , and IL 3 , with or without G CSF for 10 days , the number of clones proliferated and the number of cells per each proliferating clone was significantly less than those in normal subjects . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Preincubation with a monoclonal antibody ( MAb ) neutralizing the binding of SF to p145c kit did not affect the growth of MO7p210 cells , thus eliminating the possibility of an autocrine SF secretion . 32Dkit cells transfected with bcr / abl containing an inactivating point mutation ( Lys > Arg 271 ) in the Abl kinase domain ( 32Dp210 ( Arg 271 ) kit ) retained their responsiveness to the effects of rhSF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
TPO in combination with the ligand for c kit ( SF ) or interleukin 3 ( IL 3 ) supported colony formation by marrow cells of 5 fluorouracil ( 5 FU ) treated mice whereas TPO alone yielded no colony . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Functional analysis of the cell subpopulations , 5FU Lin ( ) Sca 1 ( + ) c kit ( + ) or normal bone marrow ( NBM ) Lin ( ) Sca 1 ( + ) c kit ( + ) CD 34 ( ) cells , indicated that exposure of these cells to recombinant human ( rh ) IL 11 in combination with steel factor ( SF ) stimulates the formation of colonies in methylcellulose and their proliferation in single cell containing liquid cultures . ^^^ RhIL 11 also synergized with both SF and IL 3 to stimulate MK colony growth from NBM Lin ( ) Sca 1 ( + ) c kit ( + ) cells ( early progenitors ) and NBM Lin ( ) Sca 1 ( ) c kit ( + ) cells ( committed late progenitors ) . ^^^ In the presence of IL 3 , NBM , Lin ( ) Sca 1 ( ) c kit ( + ) cells responded more strongly to rhIL 11 than SF . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Erythropoiesis is severely impaired in mice with inactivating mutations in the Steel factor ( SF ) gene ( Sl / Sl mice ) or in c kit , in the SF receptor gene ( W / W mice ) , and in mice with null mutations in the genes for either erythropoietin ( EPO ) or the erythropoietin receptor ( EPO R ) . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Serum SF HGF was determined using a commercially available enzyme linked immunosorbent assay kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
Soluble ICAM 1 and E sel in serum and SF were measured by a sandwich ELISA kit . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
More detailed analyses of the effects on c kit ( + ) Sca 1 ( + ) lin ( ) cells of IL 11 , SF , and FL concentrations and their interactions using response surface methodology showed IL 11 to have a maximal stimulatory effect on HSC expansion at 20 ng / mL with higher concentrations being inhibitory . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The binding of Steel factor ( SF ) to c kit initiates a signaling pathway essential for development and maintenance of interstitial cells of Cajal ( ICC ) . ^^^ The number of c kit immunoreactive cells was greater in the coculture system compared with cultures grown without SF producing fibroblasts . ^^^ All forms of SF producing fibroblasts increased ICC number in culture but physical separation of the fibroblasts from the c kit immunoreactive cells , the addition of exogenous SF to the culture medium , or fibroblast conditioned media did not . ^^^ These results are consistent with the hypothesis that the membrane bound form of SF preferentially contributes to expression of c kit positive ICC under cell culture conditions . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
The degree of redundancy between thrombopoietin ( Tpo ) and steel factor ( SF ) cytokine pathways in the regulation of hematopoiesis was investigated by generating mice lacking both c Mpl and fully functional c Kit receptors . ^^^ While Kit ( Wv / Wv ) mice , but not c Mpl ( / ) mice , are anemic , the anemia was more severe in double mutant c Mpl ( / ) Kit ( Wv / Wv ) mice , indicating redundancy between Tpo and SF in erythropoiesis . ^^^ Thus , c Mpl ( / ) Kit ( Wv / Wv ) mice reveal nonredundant and synergistic effects of Tpo and SF on primitive hematopoietic cells . . ^^^
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P10721	Pubmed	SVM Score :0.0
NA