Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.50742464
The effects on hematopoiesis of recombinant stem cell factor ( ligand for c kit ) administered in vivo to mice either alone or in combination with granulocyte colony stimulating factor . 0.50742464^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.55760218
Recombinant rat stem cell factor synergizes with recombinant human granulocyte colony stimulating factor in vivo in mice to mobilize peripheral blood progenitor cells that have enhanced repopulating potential . 0.55760218^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.86985898
Steel factor ( SF ) , the ligand for the proto oncogene c kit , acts synergistically with GM CSF or IL 3 to support the growth of normal human hematopoietic progenitor cells . 0.86985898^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.68413123
Stem cell factor ( SCF , also known as mast cell growth factor , Steel factor or Kit ligand ) has been found to synergize with GM CSF , IL 6 , IL 3 , IL 11 or Epo to increase the numbers of megakaryocyte containing colonies ( i . e . , CFU Meg , BFU Meg , CFU GMM , CFU GEMM ) . 0.68413123^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.62043646
The interactions of SCF with GM CSF , TNF , interleukin 3 ( IL 3 ) and macrophage colony stimulating factor ( M CSF ) in promoting CFU DL development have now been studied in serum free cultures of unfractionated as well as progenitor enriched cells from either bone marrow or cord blood . 0.62043646^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.54127254
Mast cell growth factor ( MGF , the ligand for c kit receptor ) can stimulate proliferation of factor dependent myeloid cell line , M07e , and MGF synergizes with granulocyte macrophage colony stimulating factor ( GM CSF ) or IL 3 in this effect . 0.54127254^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In agar culture of post 5 fluorouracil mouse bone marrow cells ( FUBM ) , recombinant rat stem cell factor ( rrSCF ) synergizes with granulocyte colony stimulating factor ( G CSF ) , interleukin 3 ( IL 3 ) or interleukin 6 ( IL 6 ) to stimulate primitive progenitor cells ( HPP CFCs ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Severe combined immunodeficient ( SCID ) mice transplanted with human bone marrow were treated with human mast cell growth factor , a fusion of interleukin 3 and granulocyte macrophage colony stimulating factor ( PIXY 321 ) , or both , starting immediately or 1 month later . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The activity of the colony stimulating factor ( SCF ) was measured in kidney subcellular fractions in mice . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The activities of a colony stimulating factor ( CSF ) , which stimulates granulocyte macrophage colony formation by mouse hemopoietic cells , and macrophage growth factor ( MGF ) , which stimulates proliferation of activated peritoneal macrophages , have been demonstrated by various criteria to reside in the same molecular species . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We show that recombinant rat SCF ( rrSCF 164 ) administered to weanling rats selectively induces an increase in thymocyte progenitor activity in the spleens of treated rats as compared to rats treated with vehicle , polyethylene glycol ( PEG ) conjugated rat albumin , or recombinant human granulocyte colony stimulating factor ( rhG CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In this report we investigated the growth of HPP CFC stimulated by combinations of interleukin ( IL ) 1 , IL 6 , kit ligand ( KL ) , granulocyte ( G ) colony stimulating factor ( CSF ) , macrophage CSF ( M CSF ) , granulocyte macrophage CSF ( GM CSF ) and IL 3 in clonal cultures incubated at 7 % or 21 % O 2 tension . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The receptors for at least two hematopoietic growth factors , namely the stem cell factor and colony stimulating factor 1 , belong to class 3 receptor tyrosine kinases . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In addition , a number of cytokines such as lymphotoxin and interferon gamma ( IFN gamma ) , are expressed exclusively in thymocytes whereas others such as stem cell factor ( SCF ) , IL 1 receptor antagonist 2 ( IRAP 2 ) and granulocyte macrophage colony stimulating factor ( GM CSF ) are produced only in stromal cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Human SCF / KL alone or in combination with the hematopoietic growth factors , interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and IL 6 , stimulated proliferation of these megakaryocytic cell lines . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
MGF alone did not promote HPP CFC colony formation by CD34+ DR CD 15 marrow cells , but synergistically augmented the ability of a combination of granulocyte monocyte colony stimulating factor ( GM CSF ) interleukin ( IL ) 3 and a recombinant GM CSF / IL 3 fusion protein ( FP ) to promote the formation of HPP CFC derived colonies . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
To determine if neoplastic human hematopoietic cells would also respond to SCF , we cultured marrow mononuclear cells from 20 patients with newly diagnosed acute myelogenous leukemia ( AML ) and two normal donors with SCF , interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , or combinations of growth factors in semisolid medium , and assessed colony growth . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Effect of stem cell factor with and without granulocyte colony stimulating factor on neonatal hematopoiesis : in vivo induction of newborn myelopoiesis and reduction of mortality during experimental group B streptococcal sepsis . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
When AML blast progenitors were grown either in the presence of fetal calf serum ( FCS ) alone or with one of the following : phytohemagglutinin leukocyte conditioned medium ( PHA LCM ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , G CSF , interleukin 3 ( IL 3 ) , or stem cell factor ( SCF ) , addition of 100 ng / mL sIL 1R or IL 1RA inhibited blast colony formation by 3 % to 96 % and 2 % to 97 % , respectively . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
SCF interacted with erythropoietin ( Epo ) and granulocyte colony stimulating factor ( G CSF ) to maintain large numbers of cells as well as to generate a twofold to threefold increase in CFC in the case of Epo , and a 10 fold increase in CFC in the case of G CSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Entry into the cell cycle of dormant hematopoietic progenitors appears to be regulated by multiple synergistic factors , including interleukin 6 ( IL 6 ) , granulocyte colony stimulating factor ( G CSF ) , IL 11 , and the ligand for c kit , which is also known as steel factor ( SF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Granulocyte macrophage colony stimulating factor ( GM CSF ) , Interleukin 3 ( IL 3 ) , and Steel Factor ( SF ) induce proliferation of hematopoietic cells through binding to specific , high affinity , cell surface receptors . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Neither granulocyte macrophage colony stimulating factor , interleukin 3 , interleukin 6 , nor the kit ligand induced the phosphorylation of the M ( r ) 75 , 000 receptor protein , although these growth factors induced the phosphorylation of other proteins . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
SCF , in combination with erythropoietin ( Epo ) , interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and granulocyte colony stimulating factor ( G CSF ) , increased the number of hematopoietic colonies formed in a semisolid medium by AA marrows . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Conversely , the ability of SCF to induce primary colony formation by AML clonogenic cells ( CFU L ) was lower than that of granulocyte macrophage colony stimulating factor ( GM CSF ) and 5637 SN in all but four cases . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In the presence of SCF alone , or in combination with granulocyte macrophage colony stimulating factor or interleukin 3 , there was less than a 17 % increase in the colony growth of Du 4475 , H 69 , or H 128 cell lines . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Bone marrow cells were plated in vitro for colony forming unit granulocyte macrophage ( CFU GM ) ( in the presence of granulocyte macrophage colony stimulating factor [ GM CSF ] ) , and for burst forming unit erythroid ( BFU E ) and CFU granulocyte , erythroid , monocyte , megakaryocyte ( CFU GEMM ) colonies ( in the presence of erythropoietin and interleukin 3 [ IL 3 ] ) , with or without 20 ng / mL of SCF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Isolated cells responded to interleukin 3 , granulocyte macrophage colony stimulating factor , mast cell growth factor , and / or granulocyte colony stimulating factor alone or in combinations in short term liquid cultures . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We have investigated the proliferation of 31 myeloid leukemia cell lines as well as fresh myeloid leukemic blasts from 17 patients in a 72 hour 3H thymidine uptake assay in the presence of various concentrations of recombinant human ( rh ) SCF alone or in combination with saturating concentrations of granulocyte macrophage colony stimulating factor ( GM CSF ) , G CSF , M CSF , interleukin 3 ( IL 3 ) , or erythropoietin ( EPO ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
A combination of 6 HGF , ie , interleukin 1 beta ( IL 1 ) , IL 3 , IL 6 , granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage CSF ( GM CSF ) , and stem cell factor ( SCF ) , was identified as the most potent combination of those tested . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We and others have recently shown that the c kit gene product is expressed on human megakaryocytes and that soluble KL / SCF in combination with granulocyte macrophage colony stimulating factor , interleukin 3 ( IL 3 ) , or IL 6 increased megakaryocyte progenitor colony formation ( CFU MEG ) and stimulated mature megakaryocytes . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
SCF increased colony forming unit granulocyte macrophage ( CFU GM ) formation from CB CD34+ cells compared to unconditioned media and had a significant additive increase with IL 3 ( p = 0 . 006 ) and granulocyte colony stimulating factor ( p = 0 . 03 ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In serum deprived conditions , no colonies were formed in the presence of macrophage colony stimulating factor ( M CSF ) , but when M CSF was combined with SCF , a marked change was noted in that large colonies were produced containing predominantly macrophages . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Non adherent cord blood and bone marrow mononuclear cells were analyzed by multiparameter flow cytometry before and at day 2 , 4 , 7 , and 11 of culture in recombinant interleukin 3 ( IL 3 ) and granulocyte colony stimulating factor ( G CSF , cord blood ) or stem cell factor ( SCF ) , IL 3 and granulocyte macrophage colony stimulating factor ( GM CSF , BM ) to assess the differentiation and maturational pathway of myeloid cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Combinations of two factors that included SF plus IL 6 , IL 11 , or granulocyte colony stimulating factor were all effective in the primary culture in the maintenance of the B lymphoid potential . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We studied the effect of MGF on the in vitro growth of clonogenic leukemic precursor cells in the presence or absence of interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and / or erythropoietin ( EPO ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
To determine if earlier hematopoietic cells remained after treatment with LLME , progenitor cell experiments were repeated with 0 . 5 mM LLME using recombinant human granulocyte macrophage colony stimulating factor ( rhGM CSF ) or recombinant human interleukin 3 ( rhIL 3 ) plus or minus recombinant human mast cell growth factor ( rhMGF ) as sources of colony stimulating activity . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
When used in combination with plateau concentrations of pokeweed mitogen mouse spleen cell conditioned medium or granulocyte macrophage colony stimulating factor ( CSF ) , MGF enhanced in greater than additive fashion colony formation by CFU GM . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Genetic and structural homology of stem cell factor and macrophage colony stimulating factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Purified natural ( n ) and recombinant ( r ) murine ( mu ) mast cell growth factor ( MGF , a c kit ligand ) were evaluated alone and in combination with r human ( hu ) erythropoietin ( Epo ) , rhu granulocyte macrophage colony stimulating factor ( rhuGM CSF ) , rhuG CSF , and / or rhuM CSF for effects in vitro on colony formation by multipotential ( colony forming unit granulocyte , erythroid , monocyte , megakaryocyte [ CFU GEMM ] ) , erythroid ( burst forming unit erythroid [ BFU E ] ) and granulocyte macrophage ( CFU GM ) progenitor cells from normal human bone marrow . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
SCF , in combination with interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , or granulocyte colony stimulating factor ( G CSF ) caused CD34+ cells to form increased numbers of granulocyte macrophage colonies ( CFU GM ) , and to form macroscopic erythroid burst forming units ( BFU E ) in the presence of IL 3 , erythropoietin ( Epo ) , and SCF . ^^^ Recombinant human stem cell factor enhances the formation of colonies by CD34+ and CD34+lin cells , and the generation of colony forming cell progeny from CD34+lin cells cultured with interleukin 3 , granulocyte colony stimulating factor , or granulocyte macrophage colony stimulating factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Combination of SCF with granulocyte , granulocyte macrophage , or multipotential colony stimulating factors , but not macrophage colony stimulating factor , resulted in enhancement of colony size . ^^^ Granulocyte colony stimulating factor enhanced cell proliferation initiated by SCF , but not vice versa , and resulted in a 10 fold increase in colony cell numbers and a 7 fold increase in progenitor cells in blast colonies . ^^^ No evidence was obtained that SCF , alone or in combination with granulocyte colony stimulating factor , could stimulate self generation by blast colony forming cells . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CD 45 cell surface antigens are linked to stimulation of early human myeloid progenitor cells by interleukin 3 ( IL 3 ) , granulocyte / macrophage colony stimulating factor ( GM CSF ) , a GM CSF / IL 3 fusion protein , and mast cell growth factor ( a c kit ligand ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Stem cell factor in combination with granulocyte colony stimulating factor ( CSF ) or granulocyte macrophage CSF synergistically increases granulopoiesis in vivo . ^^^ Recombinant rat stem cell factor ( rrSCF ) and recombinant human granulocyte colony stimulating factor ( G CSF ) coinjected for 1 week in rats cause a synergistic increase in mature marrow neutrophils accompanied by a striking decrease in erythroid and lymphoid marrow elements . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Murine mast cell growth factor ( muMGF ) , a c kit ligand , has additive to greater than additive effects on in vitro colony formation of murine and human myeloid progenitor cells stimulated with erythropoietin , granulocyte macrophage colony stimulating factor ( GM CSF ) , and / or interleukin ( IL ) 3 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
While SCF alone had little colony stimulating activity under fetal bovine serum ( FBS ) deprived culture conditions , SCF synergized with erythropoietin ( Epo ) , granulocyte / macrophage colony stimulating factor ( GM CSF ) , and interleukin 3 ( IL 3 ) to stimulate colony growth . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Individual day 14 to 18 CFU GEMM or BFU E colonies were removed from primary cultures and reseeded into secondary methylcellulose cultures containing a combination of Epo , MGF , and rhu granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
An evaluation of the effects of a recombinant , soluble form of the c kit ligand alone and in combination with either granulocyte macrophage colony stimulating factor ( GM CSF ) or interleukin 3 ( IL 3 ) on the regulation of human megakaryocytopoiesis was performed using a serum depleted clonal assay system and a long term bone marrow culture system . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In 6 of 11 cases that expressed c kit product , AML cells were found to proliferate in response to recombinant human stem cell factor ( rhSCF ) , the ligand for c kit , and the synergistic stimulation of AML cells was observed by rhSCF and granulocyte macrophage colony stimulating factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In clonal cultures of normal mouse marrow cells , combination of granulocyte , granulocyte macrophage , or multipotential colony stimulating factor ( G CSF , GM CSF , or multi CSF , respectively ) with stem cell factor ( SCF ) did not alter the number of blast colonies stimulated to develop compared with SCF alone but induced an up to 25 fold increase in their mean cell content and an up to 6 fold increase in their mean progenitor cell content . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We tested the ability of recombinant human ( rhu ) mast cell growth factor ( MGF ) , also known as c kit ligand , to stimulate the colony formation of human bone marrow cells in semisolid medium alone and in combination with rhu erythropoietin ( EPO ) , rhu Interleukin 3 ( IL 3 ) , rhu granulocyte colony stimulating factor ( G CSF ) and rhu granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Members of the receptor tyrosine kinase family bind dimeric forms of macrophage colony stimulating factor , stem cell factor and platelet derived growth factor leading to kinase activation and phosphorylation of many substrates involved in production of second messengers . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
However , in SF conditions the sequential analysis of these rGFs , alone or in combination , clearly showed a stimulating activity of interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) and erythropoietin ( EPO ) on granulopoiesis and erythropoiesis . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In vitro , SCF alone stimulated little growth of hematopoietic colony forming cells from baboon marrow , but did increase the number of colonies formed in response to erythropoietin ( Epo ) , interleukin 3 ( IL 3 ) , and granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The MO mitogenic principle of MGF was identified as the murine , macrophage specific colony stimulating factor , CSF 1 ( M CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Characterization of the cell surface receptor for a multi lineage colony stimulating factor ( CSF 2 alpha ) . 125I Labeled colony stimulating factor ( CSF ) 2 alpha ( interleukin 3 , multi CSF , and mast cell growth factor ) was used to characterize receptors specific for this lymphokine on the cell surface of the factor dependent cell line FDC P 2 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Failure to detect T cell lymphokines ( interleukin 2 and interleukin 3 ) and presence of macrophage colony stimulating factor ( CSF 1 ) and a novel mast cell growth factor in rheumatoid synovitis . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
To examine the relationship between the bone resorbing factor and other protein factors produced by spleen cells , the colony stimulating factor ( CSF ) , the differentiation inducing factor ( DIF ) , the macrophage fusion factor ( MFF ) , and the macrophage growth factor ( MGF ) were purified from 2 . 68 liters of conditioned medium of mouse spleen cell cultures treated with concanavalin A . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
A macrophages growth factor ( MGF ) was purified and , at the final step of purification on HPLC , eluted at the same position as a colony stimulating factor ( CSF ) , suggesting that MGF is identical with CSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The T cells expressed the L3T4+ Ly 2 phenotype and secreted the lymphokines : interleukin 1 ( IL 1 ) , interleukin 2 ( IL 2 ) , gamma interferon ( IFN gamma ) , colony stimulating factor ( CSF ) , mast cell growth factor ( MCGF ) and eosinophil differentiation factor ( EDF ) in response to antigen stimulation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Demonstration of WEHI 3 growth factor activity , mast cell growth factor activity , p cell stimulating factor activity , colony stimulating factor activity , and histamine producing cell stimulating factor activity . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The growth factor receptor phenotype of this immature cell fraction has been determined for granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) , and IL 6 as well as for kit ligand , making c kit an especially strong growth factor receptor marker for reconstituting stem cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Culturing of CD34+ cells for 14 days with interleukin ( IL ) 1 , IL 3 , IL 6 , stem cell factor , granulocyte macrophage colony stimulating factor ( GM CSF ) , and G CSF resulted in on average a 1 , 750 fold amplification of cell number , of which 83 % + / 7 % were MPO+ . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In tritiated thymidine incorporation assays , stem cell factor plus granulocyte macrophage colony stimulating factor stimulated thymidine incorporation in normal CD34+ cells was reduced to 72 % of control values in the presence of MIP 1 alpha , whereas incorporation by CML CD34+ cells exposed to the same factors was not altered . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In this study , we show a dose dependent response of AS 101 in the induction of high secretion levels of IL 6 , IL 3 , granulocyte macrophage colony stimulating factor ( GM CSF ) , and stem cell factor ( SCF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The combined action of stem cell factor ( SCF ) and granulocyte colony stimulating factor ( G CSF ) on granulocyte recovery and infection with Candida albicans was studied . ^^^ Effects of stem cell factor and granulocyte colony stimulating factor on granulocyte recovery and Candida albicans infection in granulocytopenic mice . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Recombinant granulocyte macrophage colony stimulating factor ( rGM CSF ) , recombinant granulocyte CSF ( rG CSF ) , recombinant macrophage CSF ( rM CSF ) , rIL 6 , rIL 11 , and recombinant stem cell factor ( rSCF ) did not alone expand nonerythroid progenitor cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
However , whereas IL 1 , IL 2 , IL 3 , IL 4 , granulocyte colony stimulating factor , and granulocyte macrophage colony stimulating factor were ineffective , IL 7 was a potent cytokine , alone or in synergy with stem cell factor ( SCF ) to induce immature thymocyte proliferation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We analyze here the amplification potential of CD34+ cord blood cells in liquid cultures stimulated with stem cell factor ( SCF ) in combination with interleukin 3 ( IL 3 ) , erythropoietin ( Epo ) or granulocyte colony stimulating factor ( G CSF ) under serum deprived conditions . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
These experiments revealed that there is an inverse correlation between the SCF R density on the cells and the doses of granulocyte macrophage colony stimulating factor ( GM CSF ) in culture medium ; the lower the dose , the higher the density of SCF R on the cells . ^^^ Granulocyte macrophage colony stimulating factor ( GM CSF ) reduces the density of stem cell factor receptors ( c kit oncogene product ) on a GM CSF dependent human myeloid cell line . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Ligand induced dimerization is a key step in the activation of receptor tyrosine kinases , including the epidermal growth factor receptor , stem cell factor receptor ( c kit ) , and colony stimulating factor 1 receptor ( c fms ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
At concentrations ranging from 0 . 1 to 10 . 0 micrograms / ml , it significantly inhibited colony forming units granulocyte macrophage ( CFU GM ) in the presence of either fetal calf serum alone , erythropoietin , leukocyte conditioned medium prepared with phytohemagglutinin , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) , or stem cell factor ( SCF ) , in a dose dependent fashion . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) was found to stimulate the growth of the haemopoietic cell line FDC P 1 in synergy with either interleukin 3 ( IL 3 ) or granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^ Stem cell factor ( SCF ) was found to stimulate the growth of the haemopoietic cell line FDC P 1 in synergy with either interleukin 3 ( IL 3 ) or granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Growth factor enhancement of CFU GM yield was obtained only by stem cell factor ( SCF ) at day 7 ; SCF , as well as granulocyte macrophage colony stimulating factor ( GM CSF ) and interleukin 3 ( IL 3 ) , had an enhancing effect at days 14 and 21 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
LTCBC initiated with CD34+HLA DR+ and CD34+HLA DR CB cells were examined over a period of 8 weeks for the production of assayable burst forming units erythroid ( BFU E ) , colony forming units granulocyte / macrophage ( CFU GM ) , and colony forming units mixed ( CFU GEMM ) in response to repeated additions of stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , IL 6 , and either erythropoietin ( Epo ) or granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The hemopoietic cytokines IL 3 ( interleukin 3 ) , IL 5 and GM CSF ( granulocyte macrophage colony stimulating factor ) are involved in the regulation of basophils ( and eosinophils ) , whereas the ligand for c kit , SCF ( stem cell factor ) is a mast cell specific agonist . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
It is known that , although SCF and bFGF have limited clonogenic activity on their own , they can augment colony stimulating factor ( CSF ) mediated progenitor cell growth . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Putative cytokines and growth factors , such as granulocyte colony stimulating factor ( CSF ) , macrophage CSF , granulocyte macrophage CSF , stem cell factor ( SCF ) , interleukin ( IL ) 6 , and IL 3 , do not possess HSC chemotactic activity . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Colony stimulating activity was provided by the recombinant human colony stimulating factors granulocyte macrophage colony stimulating factor ( GM CSF ) , granulocyte colony stimulating factor ( G CSF ) , interleukin 3 ( IL 3 ) , fusion protein ( FP ) , c kit ligand ( KL ) or GM CSF combined with other cytokines ( KL , IL 3 ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Four CD34+ cell populations , ie , ( 1 ) CD 38 , HLA DR+ , ( 2 ) CD 38 , HLA DR , ( 3 ) CD38+ , HLA DR , and ( 4 ) CD38+ , HLA DR+ cells , were sorted as single cells into 96 well flat bottom culture plates containing long term culture medium supplemented with interleukin 3 , interleukin 6 , stem cell factor ( SCF ) , granulocyte macrophage colony stimulating factor , erythropoietin , basic fibroblast growth factor ( bFGF ) , and insulin like growth factor 1 ( IGF 1 ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The effects of recombinant human stem cell factor ( SCF / c kit ligand ) , interleukin 3 ( IL 3 ) and granulocyte / macrophage colony stimulating factor ( GM CSF ) on erythroid colony formation by non phagocytic mononuclear cells ( MNC ) and CD34+ cells derived from normal human bone marrow ( BM ) , peripheral blood ( PB ) and umbilical cord blood ( CB ) were studied using a methylcellulose culture containing recombinant human erythropoietin ( Epo ) . ^^^ Different responses of human marrow and circulating erythroid progenitors to stem cell factor , interleukin 3 and granulocyte / macrophage colony stimulating factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
With a highly enriched population of these cells we have found that although GM CFC are equally responsive to macrophage colony stimulating factor ( M CSF ) and stem cell factor ( SCF ) in terms of DNA synthesis , M CSF stimulated the development of colonies containing macrophages in soft gel assays , while SCF promoted neutrophilic colony formation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Immediately after marrow transplant , the mice received SCF , interleukin 3 ( IL 3 ) , and granulocyte macrophage colony stimulating factor ( GM CSF ) alone or in combination daily for 6 days . ^^^ Synergistic effect of stem cell factor with interleukin 3 or granulocyte macrophage colony stimulating factor on the proliferation of murine primitive hematopoietic progenitors . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
When combined with subplateau concentrations ( 0 . 1 microgram / L ) of IL 3 or granulocyte macrophage colony stimulating factor , SCF had an additive or synergistic effect on clonogenic maturation of granulocyte macrophage colony forming unit and CFU MIX . ^^^ When combined with higher concentrations ( 5 . 0 micrograms / L ) of IL 3 or granulocyte macrophage colony stimulating factor , SCF generally did not enhance colony formation but did increase the number of cells per colony . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In this study , we have investigated the effects of ECM molecules , particularly of FN , on the proliferation of a myeloid leukemia cell line , M07E , which proliferates in response to either human granulocyte / macrophage colony stimulating factor ( GM CSF ) or stem cell factor ( SCF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Recombinant human SCF has major clinical potential through its synergy with other factors , especially recombinant human granulocyte colony stimulating factor , to enhance mobilization of PBPC . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The aim of this study was to analyze the potential of c kit ligand ( CKL ) combined with granulocyte macrophage colony stimulating factor ( GM CSF ) and / or interleukin 3 ( IL 3 ) to induce proliferation and differentiation of human bone marrow CD34+ cells in vitro . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The effects of recombinant canine granulocyte colony stimulating factor ( rcG CSF ) and recombinant canine stem cell factor ( rcSCF ) , a c kit ligand , on the circulation of hematopoietic progenitor and stem cells were studied in a canine model . ^^^ Effects of granulocyte colony stimulating factor and stem cell factor , alone and in combination , on the mobilization of peripheral blood cells that engraft lethally irradiated dogs . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
SCF enabled neutrophil and neutrophil / macrophage mixed progenitors to respond to granulocyte / macrophage colony stimulating factor ( GM CSF ) or interleukin 3 ( IL 3 ) and to develop the colony and further cluster formation . ^^^ The neutrophil colonies stimulated by GM CSF or IL 3 consisted mainly of immature cells , while the colonies stimulated by granulocyte colony stimulating factor ( G CSF ) consisted of mature neutrophils irrespective of the addition of SCF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , a potent colony stimulating factor , enhanced both erythroid and nonerythroid colony formation . ^^^ Stem cell factor ( SCF ) , a potent colony stimulating factor , enhanced both erythroid and nonerythroid colony formation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Furthermore , TNF alpha blocked all colony formation stimulated by SCF in combination with granulocyte colony stimulating factor ( CSF ) or CSF 1 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The number of the different haemopoietic progenitors / 1 10 10 ( 3 ) CD34+ cells , except CFU meg , was significantly higher in fetal blood than in adult bone marrow in cultures stimulated by any combination of cytokines including interleukin 3 ( IL 3 ) , granulocyte / macrophage colony stimulating factor ( GM CSF ) or stem cell factor ( SCF ) plus erythropoietin ( Epo ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Circulating haemopoietic progenitor cells from premature infants were assessed for their ability to respond to interleukin 3 , granulocyte macrophage colony stimulating factor and stem cell factor ( SCF ) in vitro . ^^^ The in vitro effects of stem cell factor , interleukin 3 and granulocyte macrophage colony stimulating factor on haemopoietic progenitor cells from premature infants . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Raf 1 is a necessary component of the mitogenic response of the human megakaryoblastic leukemia cell line MO 7 to human stem cell factor , granulocyte macrophage colony stimulating factor , interleukin 3 , and interleukin 9 . ^^^ We have examined the role of Raf 1 in the mitogenic response of the factor deprived human megakaryoblastic leukemia cell line MO 7 to recombinant human granulocyte macrophage colony stimulating factor , interleukin 3 , interleukin 9 , and stem cell factor by using c raf antisense oligodeoxyribonucleotides . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Mobilization of long term hematopoietic reconstituting cells in mice by the combination of stem cell factor plus granulocyte colony stimulating factor . ^^^ In this study , we have compared the ability of recombinant human granulocyte colony stimulating factor ( rhG CSF ) alone and the combination of low doses of recombinant rat pegylated stem cell factor ( rrSCF PEG ) plus rhG CSF to mobilize peripheral blood progenitor cells ( PBPCs ) with long term engrafting potential . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Furthermore , PB CD34+ cells were expanded in liquid culture in the presence of granulocyte macrophage colony stimulating factor ( GM CSF ) or G CSF alone or combined with interleukin 3 ( IL 3 ) , stem cell factor ( SCF ) , interleukin 1 ( IL 1 ) , interleukin 6 ( IL 6 ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Proliferation of ELF 153 cells was highly stimulated by interleukin 3 ( IL 3 ) and granulocyte macrophage colony stimulating factor and to a lesser extent by stem cell factor and IL 6 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We have studied the effects of recombinant human interleukin 9 ( IL 9 ) , alone and combined with stem cell factor ( SCF , c kit ligand ) , IL 3 , and granulocyte macrophage colony stimulating factor ( GM CSF ) on the clonogenic proliferation of highly enriched human hematopoietic CD34+ and CD34+CD33 DR progenitor cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Mast cell growth factor ( C kit ligand ) in combination with granulocyte macrophage colony stimulating factor and interleukin 3 : in vivo hemopoietic effects in irradiated mice compared to in vitro effects . ^^^ In the presence of hemopoietic cytokines such as granulocyte macrophage colony stimulating factor ( GM CSF ) and interleukin 3 ( IL 3 ) , mast cell growth factor ( MGF ; also known as steel factor , stem cell factor , and c kit ligand ) has proven to be a potent hemopoietic regulator in vitro . ^^^ Mast cell growth factor ( C kit ligand ) in combination with granulocyte macrophage colony stimulating factor and interleukin 3 : in vivo hemopoietic effects in irradiated mice compared to in vitro effects . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We treated mice with granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) to mobilize hematopoietic progenitor cells into the peripheral blood . ^^^ Efficient retrovirus transduction of mouse pluripotent hematopoietic stem cells mobilized into the peripheral blood by treatment with granulocyte colony stimulating factor and stem cell factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Pretreatment with epoxybutene in vitro suppressed recombinant interleukin 3 stimulated colony formation in haematopoietic stem and progenitor cells , had no effect on colony formation with recombinant granulocyte / macrophage colony stimulating factor or recombinant granulocyte colony stimulating factor alone , and completely blocked the synergism of recombinant c kit ligand and granulocyte / macrophage colony stimulating factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The liquid culture of PB CD34+ cells in the presence of granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) , and stem cell factor ( SCF ) resulted in a marked and long lasting increase of colony forming units granulocyte / macrophage ( CFU GM ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Based on our recent observations that recombinant rat interleukin 3 ( IL 3 ) , human interleukin 6 ( IL 6 ) and murine granulocyte macrophage colony stimulating factor ( GM CSF ) possessed different degrees of suppressive activities on the proliferation of LT 12 cell line derived from BNML rat leukemic model , SCF was evaluated alone and in combination with either IL 3 , IL 6 or GM CSF for effects on leukemopoiesis in vitro . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In vivo synergy between recombinant human stem cell factor and recombinant human granulocyte colony stimulating factor in baboons enhanced circulation of progenitor cells . ^^^ Recombinant human stem cell factor ( rhSCF ) and recombinant human granulocyte colony stimulating factor ( rhG CSF ) are synergistic in vitro in stimulating the proliferation of hematopoietic progenitor cells and their precursors . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Cells were cultured in suspension in the presence of c kit ligand , interleukin 3 , interleukin 6 , erythropoietin , and granulocyte colony stimulating factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Proliferative capacity was measured by generation of nascent myeloid progenitor cells ( granulocyte macrophage colony stimulating factor ; CFU GM ) and nucleated cells in a stroma free liquid culture stimulated by a combination of six hematopoietic growth factors ( interleukin 1 ( IL 1 ) , IL 3 , IL 6 , GM CSF , G CSF , and stem cell factor ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The combination of SCF and Epo or granulocyte macrophage colony stimulating factor ( GM CSF ) supported the production and maintenance of CFC from CD34+Rh 123low cells > 28 days compared with only 21 days for the CD34+Rh 123high cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Using clonogenic assay we investigated the effect of stem cell factor ( SCF ) on the in vitro growth of clonogenic precursor cells from acute myeloid leukemia ( AML ) and myelodysplastic syndromes ( MDS ) in the presence or absence of recombinant human erythropoietin ( rhEpo ) or recombinant human granulocyte colony stimulating factor ( rhG CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Convergence of signaling by interleukin 3 , granulocyte macrophage colony stimulating factor , and mast cell growth factor on JAK 2 tyrosine kinase . ^^^ Mast cell growth factor ( MGF ) ( also called stem cell factor ) synergizes with several lymphokines , including interleukin 3 ( IL 3 ) and granulocyte macrophage colony stimulating factor ( GM CSF ) , to promote proliferation and differentiation of certain hemopoietic progenitor cells . ^^^ Mast cell growth factor ( MGF ) ( also called stem cell factor ) synergizes with several lymphokines , including interleukin 3 ( IL 3 ) and granulocyte macrophage colony stimulating factor ( GM CSF ) , to promote proliferation and differentiation of certain hemopoietic progenitor cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
ELISA and bioassays showed that stem cell factor and interleukin 6 were produced by the HUCB stromal cell cultures , but interleukin 3 or granulocyte / macrophage colony stimulating factor was not detected . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We studied the synergistic effects of stem cell factor ( SCF ) and other burst promoting activities ( BPAs ) such as interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , or IL 9 on proliferation of human peripheral blood derived highly purified progenitors . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We have previously examined the effects of granulocyte colony stimulating factor ( G CSF ) alone or in combination with either stem cell factor ( SCF ) or interleukin 11 ( IL 11 ) on in vivo neonatal rat hematopoiesis . ^^^ The combined effects of interleukin 11 , stem cell factor , and granulocyte colony stimulating factor on newborn rat hematopoiesis : significant enhancement of the absolute neutrophil count . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The current study asked whether survival could be further improved by treating allograft recipients with recombinant canine granulocyte colony stimulating factor ( G CSF ) , stem cell factor ( SCF ) , or G CSF / SCF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
For its effect , SCF requires the synergistic action of erythropoietin ( Epo ) , granulocyte colony stimulating factor ( G CSF ) , or interleukin 3 ( IL 3 ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We sorted single cells from four populations of CD34+ cells from fetal bone marrow : ( 1 ) CD 38 HLA DR , ( 2 ) CD 38 HLA DR+ , ( 3 ) CD38+ HLA DR , and ( 4 ) CD38+ HLA DR+ into liquid culture media supplemented with interleukin 3 ( IL 3 ) IL 6 , stem cell factor ( SCF ) , granulocyte macrophage colony stimulating factor , erythropoietin , basic fibroblast growth factor ( bFGF ) and insuline like growth factor ( IGF 1 ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
RNA analysis showed that stromal cells transcribed , in addition to SCF , messages for granulocyte colony stimulating factor ( G CSF ) , granulocyte monocyte CSF ( GM CSF ) , interleukin 6 ( IL 6 ) , and transforming growth factor beta ( TGF beta ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Rapid engraftment by peripheral blood progenitor cells mobilized by recombinant human stem cell factor and recombinant human granulocyte colony stimulating factor in nonhuman primates . ^^^ We have previously shown that administration of low dose recombinant human stem cell factor ( rhSCF ) plus recombinant human granulocyte colony stimulating factor ( rhG CSF ) to baboons mobilizes greater numbers of progenitor cells in the blood than does administration of rhG CSF alone . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
To examine the role of stem cell factor ( SCF ) or its receptor in this disorder , we investigated the stimulatory effects of recombinant canine SCF ( rc SCF ) on in vitro marrow cultures , cloned and sequenced the grey collie SCF gene , and treated three grey collies with rc SCF , either alone or in combination with recombinant canine granulocyte colony stimulating factor ( rcG CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Maximal numbers of granulocyte monocyte progenitors in mononuclear cell cultures , could be achieved with a mixture of stem cell factor , interleukin 3 and granulocyte monocyte colony stimulating factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Flow cytometric analysis of five myeloma cell lines ( RPMI 8226 , ARH 77 , KMM 1 , U 266 , and Hs ) and myeloma cells freshly isolated from eight patients showed that interleukin 1 receptor ( IL 1R ) type 1 and type 2 , IL 2R alpha and beta chains , IL 4R , IL 6R , IL 7R , IL 8R , granulocyte macrophage colony stimulating factor receptor ( GM CSFR ) , c kit ( stem cell factor receptor [ SCFR ] ) , membrane bound stem cell factor ( MBSCF ) , and tumor necrosis factor ( TNF ) receptors type 1 and type 2 were not always detected on the myeloma cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Mutual inhibition of murine erythropoiesis and granulopoiesis during combined erythropoietin , granulocyte colony stimulating factor , and stem cell factor administration : in vivo interactions and dose response surfaces . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Cells were cultured in a suspension system for 3 days in the presence of stem cell factor and interleukin 3 ( IL 3 ) , after which granulocyte colony stimulating factor ( G CSF ) was added . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Synergism between FL and either granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) or KIT ligand ( KL ) was observed in promoting the growth of high proliferative potential ( HPP ) colony forming cells ( CF ) and / or low proliferative potential ( LPP ) CFC in cultures of CD34++ CD38+ Lin and CD34++ CD 38 Lin LDFL cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Cell to cell interaction of cytokine dependent myeloblastic line constitutively expressing membrane bound stem cell factor abrogates cytokine dependency partially through granulocyte macrophage colony stimulating factor production . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Modulation of human cord blood progenitor cell growth by recombinant human interleukin 3 ( IL 3 ) , IL 6 , granulocyte macrophage colony stimulating factor ( GM CSF ) and stem cell factor ( SCF ) in serum supplemented and serum free medium . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The selected cells were expanded for two weeks in Iscove ' s medium supplemented with 20 % FBS and various combinations of interleukin 3 ( IL 3 ) , granulocyte colony stimulating factor ( G CSF ) , stem cell factor ( SCF ) and interleukin 6 ( IL 6 ) added either simultaneously or sequentially . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The proliferation kinetics and clonogenic activity of CD34+ / 38hi ( CD38hi ) and CD34+ / 38lo ( CD38lo ) human marrow cells were measured before and after culturing the cells in vitro over a 6 day period in serum deprived medium containing recombinant growth factors ( interleukin 1 [ IL 1 ] , IL 3 , IL 6 , granulocyte colony stimulating factor [ G CSF ] , granulocyte macrophage [ GM ] CSF , kit ligand , and erythropoietin ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Polymerase chain reaction analysis of Saka cell RNA detected expression of messenger RNAs for interleukin 6 ( IL 6 ) , IL 1 beta , granulocyte macrophage colony stimulating factor , macrophage colony stimulating factor , stem cell factor , and the 1 , 25 dihydroxyvitamin D 3 receptor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
For instance , leukemia , inhibitor factor and stem cell factor act on pluripotent cells and immature progenitors , granulocyte macrophage colony stimulating factor ( GM CSF ) acts at early stages of the development of myelomonocytic lineage , whereas granulocyte colony stimulating factor ( G CSF ) and macrophage colony stimulating factor ( M CSF ) act on more mature cells of the same lineage and are only required later during the differentiation of this cell lineage . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The use of powerful combinations of newer growth factors , such as interleukin 3 with granulocyte macrophage colony stimulating factor ( GM CSF ) , or granulocyte colony stimulating factor ( G CSF ) with stem cell factor , may obviate the need for chemotherapy . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Granulocyte colony stimulating factor , granulocyte macrophage colony stimulating factor , PIXY 321 , stem cell factor , interleukin 3 , and interleukin 7 : receptor binding and effects on clonogenic proliferation in acute lymphoblastic leukemia . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Specifically , TNF alpha is a potent inhibitor of the clonal growth of primitive and committed murine and human bone marrow progenitors in combination with multiple cytokines , including granulocyte colony stimulating factor ( G CSF ) , CSF 1 , erythropoietin ( Epo ) , stem cell factor ( SCF ) , and flt 3 ligand ( FL ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Whereas FL was a weak proliferative stimulator alone , it potently synergized with a number of other HGFs , including all four colony stimulating factor ( CSF ) , interleukin ( IL ) 6 , IL 11 , IL 12 , and stem cell factor ( SCF ) , to promote the colony formation of Lin Sca 1+ , but not Lin Sca 1 or erythroid progenitor cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In the present study , we investigated the capacity of porcine microvascular endothelial cells ( PMVECs ) together with combinations of cytokines ( granulocyte macrophage colony stimulating factor [ GM CSF ] + stem factor [ SCF ] , interleukin 3 [ IL 3 ] + SCF + IL 6 , and GM CSF + IL 3 + SCF + IL 6 ) to support the expansion and development of purified human CD34+ bone marrow cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The granulomonocytic colony forming unit ( CFU GM ) assay measures committed progenitors responsive to a single species of colony stimulating activity such as granulocyte macrophage colony stimulating factor ( GM CSF ) , whereas the pre CFU assay identifies more primitive progenitors by measuring interleukin 3 ( IL 3 ) and kit ligand ( KL ) induced generation of secondary CFU GM from CD34+ , 4 hydroperoxycyclophosphamide resistant progenitors that require multiple cytokine stimuli . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Between days 5 and 7 of culture , CD34+PKH2bright and CD34+PKH2dim cells were sorted in a limiting dilution scheme into 96 well plates prepared with medium , SCF , IL 3 , IL 6 , granulocyte macrophage colony stimulating factor , and erythropoietin . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In the presence of fetal bovine serum ( FBS ) , IL 12 increased the number of colony forming units for granulocyte macrophage ( CFU GM ) from CD34+33 cells which responded to IL 3 , but not the number of CFU GM from those which responded to stem cell factor ( SCF ) or granulocyte colony stimulating factor ( G CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Peripheral blood progenitor cells mobilized by recombinant human granulocyte colony stimulating factor plus recombinant rat stem cell factor contain long term engrafting cells capable of cellular proliferation for more than two years as shown by serial transplantation in mice . ^^^ Transplantation of recombinant human granulocyte colony stimulating factor ( rhG CSF ) plus recombinant rat stem cell factor ( rrSCF ) mobilized PBPC resulted in 98 % donor engraftment of primary recipients at 12 to 14 months post transplantation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Specificity of CD 64 for the granulo monocytic lineage was shown by demonstrating that colonies arising from CD34+ , CD64+ cells consisted of 98 % + / 2 % colony forming unit granulocyte macrophage ( CFU GM ) in semisolid medium containing stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , IL 6 , granulocyte macrophage colony stimulating factor ( GM CSF ) , and erythropoietin ( EPO ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Stem cell factor and interleukin 6 , alone or in combination with granulocyte colony stimulating factor , do not affect the growth of solid tumor cell lines . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
When stem cells were stimulated in liquid culture with FL plus interleukin ( IL ) 3 , IL 6 , granulocyte colony stimulating factor ( G CSF ) , or stem cell factor ( SCF ) , cells capable of forming colonies in secondary methylcellulose cultures ( CFU c ) were produced in high numbers . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Hematopoietic progenitor assays for unseparated ( cell concentration 3 10 10 ( 4 ) , 1 10 10 ( 5 ) , 3 10 10 ( 5 ) ) and CD 34 enriched cells ( cell concentration 1 10 10 ( 3 ) , 3 10 10 ( 3 ) , 1 10 10 ( 4 ) ) were performed in the presence of 5 U human interleukin 3 ( hu IL 3 ) , 30 U hu IL 6 , 10 U human granulocyte colony stimulating factor , 6 U erythropoietin , and 15 ng human stem cell factor . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Expansion of immunostimulatory dendritic cells among the myeloid progeny of human CD34+ bone marrow precursors cultured with c kit ligand , granulocyte macrophage colony stimulating factor , and TNF alpha . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We conclude from these findings that PHSCs are programmed to interact with stem cell factor , IL 3 , and IL 6 but not with granulocyte or macrophage colony stimulating factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
SPY 3 2 expressed high levels of c kit ligand and low levels of granulocyte macrophage colony stimulating factor and interleukin 3 , and these molecules were all involved in this long term erythropoiesis . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Diversity and similarity in signaling pathways of granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) , and stem cell factor ( SCF ) in five human factor responsive leukemia cell lines were investigated by immunoblotting to detect tyrosine phosphorylation of intracellular proteins . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Northern blots of LTBMC adherent layers obtained after in vitro treatment with AM 5 revealed a significant mRNA expression for interleukin 1 alpha ( IL 1 alpha ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , macrophage CSF ( M CSF ) and granulocyte CSF ( G CSF ) , in contrast with adherent layers from untreated cultures which only expressed , in detectable levels , M CSF and stem cell factor ( SCF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
When employed with cytokines regulating DC development ( tumor necrosis factor [ TNF ] + GM colony stimulating factor [ GM CSF ] ) , SCF increased the size of monocyte ( mono ) and mono DC colonies arising from cord blood CD34+ progenitor cells . ^^^ Stem cell factor augments tumor necrosis factor granulocyte macrophage colony stimulating factor mediated dendritic cell hematopoiesis . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In contrast to the vigorous proliferation in vivo , CML N 1 cells from any passage failed to proliferate in vitro under standard liquid culture conditions with or without growth factors , such as granulocyte macrophage colony stimulating factor , granulocyte colony stimulating factor , monocyte colony stimulating factor , interleukin 3 , interleukin 6 and stem cell factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Binding of the cytokines interleukin ( IL ) 1 alpha , IL 3 , IL 6 , stem cell factor ( SCF ) , macrophage inhibitory protein ( MIP ) 1 alpha , granulocyte colony stimulating factor ( G CSF ) , and granulocyte macrophage ( GM ) CSF to these CD34+ cell populations was also analyzed by flow cytometry . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Canine MGDF is characterized as a human megakaryocyte colony stimulating factor that acts synergistically with human recombinant stem cell factor but not interleukin 3 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Further flow cytometric analysis using biotinylated growth factors showed that the IL 3R+ basophils also expressed receptors for granulocyte macrophage colony stimulating factor ( GM CSF ) , but not for IL 6 or Kit ligand . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Furthermore , rhTPO induced proliferation of AML cells was augmented with the addition of interleukin 3 ( IL 3 ) , IL 6 , stem cell factor , or granulocyte macrophage colony stimulating factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In vitro , SCF synergizes with other growth factors , such as granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor , and interleukin 3 to stimulate the proliferation and differentiation of cells of the lymphoid , myeloid , erythroid , and megakaryocytic lineages . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
OBJECTIVE : To determine fetal serum and amniotic fluid ( AF ) levels of interleukin ( IL ) 3 , IL 6 , granulocyte macrophage colony stimulating factor , stem cell factor , and erythropoietin , and to explore the relationship between cytokines and hemoglobin concentration , white blood cell count ( WBC ) , and platelet count in fetuses affected by Rh immunization . ^^^ The levels of IL 3 , IL 6 , granulocyte macrophage colony stimulating factor , stem cell factor , and erythropoietin were estimated using commercially available immunoassays . ^^^ Fetal serum contained higher concentrations of IL 3 , granulocyte macrophage colony stimulating factor , stem cell factor , and erythropoietin compared with AF . ^^^ Other studied cytokines ( such as stem cell factor , granulocyte macrophage colony stimulating factor , and IL 6 ) did not correlate with the degree of fetal anemia . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Ex vivo expansion of peripheral blood mononuclear cells ( MNCs ) , cultured both directly and after selection for CD34+ cells , was compared in static and continuously perfused cultures containing interleukin ( IL ) 3 , IL 6 , granulocyte colony stimulating factor ( G CSF ) , and stem cell factor ( SCF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
TM 8 cells expressed certain levels of macrophage colony stimulating factor and stem cell factor messenger RNAs ( mRNAs ) , but low levels of c fms mRNA . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We also demonstrated that , in the presence of interleukin 3 , interleukin 6 , granulocyte colony stimulating factor , and erythropoietin , TGF beta 1 antisense oligonucleotides or anti TGF beta serum have an additive effect with KIT ligand ( Steel factor [ SF ] ) , which suggests that they control different pathways of regulation in these conditions . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The same RA patient also showed a marked increase in myeloid colony numbers , which were undetectable with granulocyte macrophage colony stimulating factor alone , but within the normal range when SCF was added . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The effect of basic fibroblast growth factor ( bFGF ) alone and in combination with other hematopoietic growth factors on the colony formation of K 562 human leukemic cells was studied using soft agar colony assay . bFGF was found to have a weak colony stimulating activity on K 562 cells derived from the blastic crisis cells of human chronic myelogenous leukemia and to potentiate the K 562 cell colony stimulating activity of granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) and stem cell factor ( SCF ) at a low concentration of below 1 ng / ml . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The exogenously added GFs interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , stem cell factor ( SCF ) , and erythropoietin ( Epo ) each exhibited different , but reproducible , consumption kinetics . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
MGF alone had a statistically significant stimulatory effect on GM colony formation , as also did interleukin 3 ( IL 3 ) and GM colony stimulating factor ( GM CSF ) , although the progenitors could form colonies spontaneously as well . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The SCID hu mice were treated with a cytokine mixture ( recombinant human stem cell factor , interleukin 3 , granulocyte / macrophage colony stimulating factor , and granulocyte colony stimulating factor ) for 4 months and were then maintained for further 8 months under cytokine free conditions . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The FLT 3 gene encodes a receptor tyrosine kinase that is closely related to two well known receptors , KIT and FMS , that regulate with their respective ligands , stem cell factor ( SCF ) and macrophage colony stimulating factor ( M CSF ) , proliferation and differentiation of hematopoietic cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Macrophage colony stimulating factor ( M CSF ) , interleukin 3 ( IL 3 ) , PIXY 321 , stem cell factor ( SCF ) , IL 1 , IL 6 , and IL 11 represent cytokines not yet approved ; the majority of these newer agents have their principal action at an earlier time point in the hematopoietic cascade than the currently approved cytokines . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Effects of recombinant canine stem cell factor , a c kit ligand , and recombinant granulocyte colony stimulating factor on hematopoietic recovery after otherwise lethal total body irradiation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Colony formation was assessed in 1 degree wells containing various cytokines , alone and in combination , and single colonies deriving after 21 days in semisolid medium were replated into 2 degree wells in the presence of the combination of purified preparations of recombinant human steel factor ( SF , a c kit ligand ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , granulocyte colony stimulating factor ( G CSF ) , interleukin 3 ( IL 3 ) , and erythropoietin ( Epo ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The ability of CD34+ / c kit+ cells to form multilineage colonies ( CFU GEMM ) was assayed after sorting with an antibody that did not show any significant effect on c kit ligand ( RL ) or granulocyte / macrophage colony stimulating factor ( GM CSF ) induced colony formation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In clonogenetic dishes , graded amounts of c kit ligand ( KL ) were added together with saturating levels of erythropoietin ( Ep ) and variable amounts of interleukin 3 and granulocyte macrophage colony stimulating factor ( IL 3 / GM CSF ) , ie , high or low level , or no IL 3 / GM CSF addition . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Cell line stimulation and antibody blocking indicated the presence of c kit ligand , interleukin 6 ( IL 6 ) and granulocyte colony stimulating factor ( G CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The optimum growth factor combination of conditioned medium from Mia PaCa 2 cells ( Mia CM ) , recombinant granulocyte macrophage colony stimulating factor and recombinant stem cell factor ( SCF ) supported the formation of macroscopic ( > or = mm ) colonies ( 97 % of which were multilineage ) , at an average incidence of 250 / 10 ( 5 ) MNC . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Interleukin 6 ( IL 6 ) , granulocyte colony stimulating factor ( G CSF ) , interleukin 11 ( IL 11 ) , interleukin 4 ( IL 4 ) , interleukin 1 alpha ( IL 1 alpha ) , and tumor necrosis factor alpha ( TNF alpha ) also supported survival of a few progenitors , but much less effectively than either IL 3 or SF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
To that effect , stromal cell free LTBMC were initiated with CD34+ HLA DR CD 15 rhodamine 123dull bone marrow cells and were maintained with repeated addition of c kit ligand and a synthetic interleukin 3 / granulocyte macrophage colony stimulating factor fusion protein . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Further , Northern analysis showed that the preadipocyte cell line acquired the ability to produce a significant amount of stem cell factor ( SCF ) , interleukin 6 ( IL 6 ) , leukemia inhibitory factor , and macrophage colony stimulating factor mRNAs in response to IL 1 or lipopolysaccharide stimulation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
MGF synergized with interleukin 3 ( IL 3 ; four out of 10 cases ) , granulocyte macrophage colony stimulating factor ( GM CSF ; three out of 10 cases ) , granulocyte colony stimulating factor ( G CSF ; six out of 10 cases ) , macrophage colony stimulating factor ( M CSF ; one out of 10 cases ) and erythropoietin ( EPO ; one out of 10 cases ) when added to culture in combination . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In both colony stimulating factor 1 and stem cell factor stimulated cells , there is a marginal increase in tyrosine phosphorylation of HCP . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Four cytokine combinations were tested : ( 1 ) mast cell growth factor ( MGF ; a c kit ligand ) and interleukin 6 ( IL 6 ) ; ( 2 ) MGF , IL 6 , IL 3 , and erythropoietin ( Epo ) ; ( 3 ) MGF , IL 6 , granulocyte macrophage colony stimulating factor ( GM CSF ) / IL 3 fusion protein ( FP ) , macrophage colony stimulating factor ( M CSF ) , and granulocyte CSF ( G CSF ) ; and ( 4 ) MGF , IL 6 , FP , M CSF , G CSF , and Epo . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
However , SCF , in combination with interleukin 3 ( IL 3 ) or with `` late acting factors ' ' ( granulocyte colony stimulating factor ( G CSF ) or erythropoietin ( Epo ) ) , generated large numbers of mature cells as well as CFC . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CD34+ cells enriched from bone marrow were cultured for up to 5 weeks in interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) and granulocyte colony stimulating factor ( G CSF ) with or without stem cell factor ( SCF ) ( also termed c kit ligand ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In liquid cultures , recombinant human stem cell factor ( SCF ) , in combination with interleukin 1 ( IL 1 ) , IL 3 , IL 6 , granulocyte macrophage colony stimulating factor , or granulocyte colony stimulating factor caused untreated , but not 4HC treated , CD34+lin cells to form CFC . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) acts in synergy with other growth factors such as erythropoietin ( Epo ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , or interleukin 3 ( IL 3 ) , to stimulate the growth of primitive hematopoietic cells . ^^^ Stem cell factor ( SCF ) acts in synergy with other growth factors such as erythropoietin ( Epo ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , or interleukin 3 ( IL 3 ) , to stimulate the growth of primitive hematopoietic cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Treatment of these cells with c myb antisense oligodeoxynucleotides caused downregulation of c myb expression , decreased in the number of marrow CFU F colonies ( approximately 54 % inhibition ) and in the cell number within residual colonies ( approximately 80 % ) , and downregulation of granulocyte / macrophage colony stimulating factor ( GM CSF ) and stem cell factor ( SCF ) mRNA expression . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Antibodies against interleukin 1 ( IL 1 ) , IL 3 , granulocyte macrophage colony stimulating factor ( GM CSF ) , and Epo could not abolish the enhancing effect of MGF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
MGF alone or in combination with MoT cell line conditioned medium ( MoCM ) or granulocyte macrophage colony stimulating factor ( GM CSF ) + interleukin 3 ( IL 3 ) significantly increased the proliferative capacity of erythropoietin ( EPO ) dependent CML and normal BFU E . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
All four tumors stained positively for stem cell factor ( SCF ) , granulocyte colony stimulating factor ( G CSF ) , interleukin 2 ( IL 2 ) , IL 4 , IL 5 , interferon alpha ( IFN alpha ) , and insulin like growth factor 1 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
These studies show that culture of DR cells for 5 weeks in a `` stroma free ' ' culture supplemented with a combination of four early acting cytokines ( Interleukin 3 [ IL 3 ] , stem cell factor [ SCF ] , leukemia inhibitory factor [ LIF ] , and granulocyte colony stimulating factor [ G CSF ] ) results in a similar cell expansion as when DR cells are cultured in `` stroma noncontact ' ' cultures supplemented with the same cytokines . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
However , combination of SF with lineage specific factors , such as erythropoietin ( Epo ) or / and granulocyte colony stimulating factor ( G CSF ) was not sufficient for the proliferation of multipotential progenitors ( CFU GEMM ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Combination in marrow cultures of SCF and granulocyte colony stimulating factor ( G CSF ) increased cell numbers more than tenfold in developing granulocytic and blast cell colonies . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Ten healthy donors and four patients with Diamond Blackfan anemia ( DBA ) have been investigated for granulocyte macrophage colony stimulating factor ( GM CSF ) and stem cell factor ( SCF ) production by bone marrow enriched fibroblasts ( BMEF ) in a highly sensitive biological assay on growth factor dependent M 07e cells . ^^^ M 07e cell bioassay detects stromal cell production of granulocyte macrophage colony stimulating factor and stem cell factor in normal and in Diamond Blackfan anemia bone marrow . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Since either an early block of differentiation in stem cells or their insufficient support by stromal functions could be an underlying factor , levels of stem cell factor ( SCF ) and cytokines have been measured in blood and in supernatants of monocytes after stimulation with granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Addition of SCF ( 50 ng / ml ) to marrow cultures stimulated with the standard mixture of growth factors ( interleukin 3 or IL 3 , granulocyte macrophage colony stimulating factor or GM CSF , and erythropoietin or Epo ) significantly increased the mean ( + / SD ) concentration of mafosfamide inducing 95 % inhibition of CFU GM ( 106 + / 17 versus 130 + / 29 , p < or = . 0005 ) , but not granulocyte / erythroid / macrophage / megakaryocyte CFU ( CFU GEMM ) ( 85 + / 4 versus 90 + / 1 , p < or = . 1 ) and BFU E ( 90 + / 5 versus 92 + / 5 , p < or = . 1 ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Stem cell factor enhances in vivo effects of granulocyte colony stimulating factor for stimulating mobilization of peripheral blood progenitor cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In both murine and human systems the c kit ligand , also known as mast cell growth factor ( MGF ) , acts synergistically with several colony stimulating factors , including the granulocyte macrophage colony stimulating factor ( GM CSF ) and interleukin 3 ( IL 3 ) , in stimulating the proliferation and differentiation of different types of hematopoietic progenitors . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
When added to interleukin 3 ( IL 3 ) and / or granulocyte macrophage colony stimulating factor ( GM CSF ) , SCF has an especially profound effect on the in vitro proliferation of several classes of primitive hematopoietic progenitor cells including the burst forming unit megakaryocyte ( BFU MK ) , the high proliferative potential colony forming cell ( HPP CFC ) and the long term bone marrow culture initiating cell ( LTBMC IC ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Other growth factors such as PIXY , interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) and granulocyte CSF ( G CSF ) and the combination of MGF plus PIXY were also tested . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We tested the hypothesis that the Fanconi anemia mutation results in insufficient production of hematopoietic growth factors by stromal cells by quantifying constitutive and induced production of interleukin 6 ( IL 6 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , granulocyte colony stimulating factor ( G CSF ) , macrophage colony stimulating factor ( M CSF ) , and steel factor ( SF ) by untransformed fibroblasts from eight patients with FA from five different families . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The addition of granulocyte macrophage colony stimulating factor ( GM CSF ) , IL 6 , stem cell factor ( SCF ) , or leukemia inhibitory factor ( LIF ) to low levels of IL 3 also stimulated megakaryocyte growth , as measured by IIb / IIIa expression . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Several investigational rhHGFs , including interleukin 3 , 6 , and 11 , PIXY 321 ( a granulocyte macrophage colony stimulating factor / interleukin 3 hybrid ) , and kit ligand ( also known as `` stem cell factor ' ' ) , have the potential for affecting multiple lineages . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Effect of c kit ligand ( stem cell factor ) in combination with interleukin 5 , granulocyte macrophage colony stimulating factor , and interleukin 3 , on eosinophil lineage . ^^^ Although interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and interleukin 5 ( IL 5 ) each exhibited eosinophil colony stimulating activity , SCF did not do so alone . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Severe congenital neutropenia : abnormal growth and differentiation of myeloid progenitors to granulocyte colony stimulating factor ( G CSF ) but normal response to G CSF plus stem cell factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Effect of stem cell factor ( c kit ligand ) , granulocyte macrophage colony stimulating factor and interleukin 3 on hematopoietic progenitors in human long term bone marrow cultures . ^^^ We evaluate the effects of recombinant human stem cell factor ( SCF or c kit ligand ) alone and also in combination with recombinant human granulocyte macrophage colony stimulating factor ( GM CSF ) and interleukin 3 ( IL 3 ) , on cell proliferation and differentiation in human long term bone marrow cultures ( LTBMC ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We have studied the effects of recombinant human interleukin 11 ( rhIL 11 ) , alone and combined with stem cell factor ( SCF or c kit ligand ) , IL 3 , and granulocyte macrophage colony stimulating factor ( GM CSF ) on the proliferation of highly enriched human hematopoietic CD34+ and CD34+CD33 DR progenitor cells . ^^^ Interleukin 11 stimulates the proliferation of human hematopoietic CD34+ and CD34+CD33 DR cells and synergizes with stem cell factor , interleukin 3 , and granulocyte macrophage colony stimulating factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Consistent gene expression in the BM of all 7 individuals was detected for macrophage colony stimulating factor ( CSF ) , stem cell factor , interleukin 6 ( IL 6 ) , IL 7 , erythroid potentiating factor , erythroid differentiating factor , and insulinlike growth factor 1 , all cytokines with reported direct stimulatory effects on in vitro hematopoiesis . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Primitive hematopoietic progenitors were stimulated by early acting stimulatory factors such as SCF , IL 11 , IL 9 and the intermediate late acting growth factors IL 3 and granulocyte macrophage colony stimulating factor ( GM CSF ) , alone and in combination . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
LDA11 cells can be induced with lipopolysaccharide ( LPS ) , IL 1 alpha , or interferon gamma ( IFN gamma ) to express mRNA for monocyte chemoattractant protein 1 ( MCP 1 / JE ) , IFN inducible protein 10 ( IP 10 ) , stem cell factor ( SCF ) , and macrophage colony stimulating factor ( M CSF ) but not for IL 1 alpha , IL 3 , or tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In addition , CML and normal stromal supernatants contained similar quantities of both growth stimulatory ( granulocyte colony stimulating factor ( CSF ) , interleukin 6 , stem cell factor , granulocyte macrophage CSF , and interleukin 1 beta ) and growth inhibitory cytokines ( transforming growth factor beta , macrophage inflammatory protein 1 alpha , and tumor necrosis factor alpha ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Bone marrow cells from each of two inbred mouse strains , C57BL / 6J and DBA / 2J , were placed into separate bioreactor cultures perfused continuously with growth medium containing erythropoietin ( Epo ) , interleukin 3 ( IL 3 ) , granulocyte macrphage colony stimulating factor ( GM CSF ) , and Kit ligand as well as 5 % CO 2 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We therefore searched for immediate early cytokine responsive genes and isolated a novel gene , CIS ( cytokine inducible SH 2 containing protein ) which is induced in hematopoietic cells by a subset of cytokines including interleukin 2 ( IL 2 ) , IL 3 , granulocyte macrophage colony stimulating factor ( GM CSF ) and erythropoietin ( EPO ) , but not by stem cell factor , granulocyte colony stimulating factor and IL 6 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Purified CD 34 positive hematopoietic progenitors were uPAR negative , but expressed uPAR during differentiation in short term liquid culture stimulated in vitro by recombinant interleukin ( IL ) 1 , IL 3 , IL 6 , granulocyte macrophage colony stimulating factor ( CSF ) , granulocyte CSF , and stem cell factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
A new serum free culture ( SFC ) system for human AML CFU was established and the colony promoting activity of four recombinant human hematopoietic growth factors ( rhHGFs ) including granulocyte macrophage colony stimulating factor ( rhGM CSF ) , interleukin 3 ( rhIL 3 ) , erythropoietin ( rhEPO ) and newly developed stem cell factor ( rhSCF ) were investigated in this SFC system . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Although the combination of SF , IL 7 , and Ep was sufficient to support formation of lymphomyeloid colonies , addition of IL 11 , granulocyte colony stimulating factor or IL 12 to the combination of SF , IL 7 , and Ep increased the number of lymphomyeloid colonies . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Low numbers of day 9 EB cells ( 5 10 10 ( 5 ) or less ) cultured in the presence of several growth factors ( interleukin 3 [ IL 3 ] , IL 1 , c kit ligand , basic fibroblast growth factor , insulin growth factor 1 , IL 6 , granulocyte colony stimulating factor , fetal liver kinase 2 ligand ) develop few or no CFCs after 1 week of culture . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Furthermore , fraxiparin was found to potentiate the stimulating activity of aplastic anaemia serum ( AAS ) but not stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) and erythropoietin ( Epo ) , on MK colony growth in vitro , and to neutralize the inhibitory effect of platelet factor 4 ( PF 4 ) in vitro and in vivo . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Two cell lines , HS 5 and HS 21 , appear to be fibroblastoid and secrete significant levels of granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage CSF ( GM CSF ) , macrophage CSF ( M CSF ) , Kit ligand ( KL ) , macrophage inhibitory protein 1 alpha , interleukin 6 ( IL 6 ) , IL 8 , and IL 11 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
METHODS We measured IL 6 , IL 1 beta , tumour necrosis factor alpha ( TNF alpha ) , granulocyte macrophage colony stimulating factor , IL 8 , and polymorphonuclear leucocyte ( PMNL ) chemotaxis and degranulation in SF from patients with RA ( n = 30 ) in the early phase of the disease . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We also showed that 5 FU resistant PBSC have a greater capacity for expansion of IL 1 / IL 3 / SCF responsive immature progenitors ( p = 0 . 05 ) , amplification of IL 3 plus GM CSF responsive progenitors ( p = 0 . 01 ) , and production of committed single growth factor responsive ( granulocyte macrophage colony stimulating factor [ GM CSF ] ) precursors ( p = 0 . 01 ) than the untreated PBSC . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
To identify the possible factors responsible for the activities detected in the keratinocyte conditioned media , we performed northern blot analysis using the cytokine probes granulocyte colony stimulating factor , macrophage colony stimulating factor , granulocyte macrophage colony stimulating factor , stem cell factor , interleukin 1 alpha , interleukin 3 , and tumor necrosis factor alpha . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Allergen inhalation increased airway responsiveness ( P < 0 . 001 ) and the number of granulocyte macrophage colony forming units ( CFU ) when cultured with dog serum and either recombinant canine stem cell factor ( rcSCF ) ( P < 0 . 001 ) or granulocyte colony stimulating factor ( rcG CSF ) ( P = 0 . 035 ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We have monitored the serum concentrations of hematopoietic growth factors ( HGFs ; ie , stem cell factor [ SCF ] , leukemia inhibitory factor [ LIF ] , interleukin 3 [ IL 3 ] , IL 6 , IL 8 , and granulocyte colony stimulating factor [ G CSF ] ) in 15 lymphoma / leukemia and 6 ovarian cancer patients undergoing autologous bone marrow ( BM ) or peripheral blood ( PB ) stem cell transplantation ( SCT ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
With tumor necrosis factor ( TNF ) + granulocyte macrophage colony stimulating factor ( GM CSF ) + stem cell factor treatment numerous colony like clusters developed . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Supplementation of serum free medium with recombinant cytokines , including stem cell factor , granulocyte macrophage colony stimulating factor ( GM CSF ) , and tumor necrosis factor ( TNF ) alpha or with human placenta conditioned medium ( HPCM ) matched the degree of inhibition of apoptosis induced by BMF in only 50 % of cases . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Second , it is demonstrated that there is no granulocyte monocyte colony stimulating factor ( GM CSF ) , CSF 1 , interleukin 3 ( IL 3 ) and possibly stem cell factor ( SCF ) production by the marrow cells during the contact . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We cultured M TAT cells long term ( > 1 year ) in the continuous presence of erythropoietin ( EPO ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , or stem cell factor ( SCF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Early and late normal progenitor / stem cells were studied in the presence of placenta conditioned medium ( placenta conditioned medium granulocyte macrophage colony forming units ) ; in the presence of granulocyte colony stimulating factor , granulocyte macrophage colony stimulating factor , interleukin 3 , erythropoietin , stem cell factor ( 5R CFU GM ) ; stimulated granulocyte macrophage colony forming and in a long term culture initiating cell assay . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Reverse transcriptase polymerase chain reaction showed that IL 3 , IL 4 , IL 6 , stem cell factor , granulocyte colony stimulating factor , and granulocyte / macrophage colony stimulating factor mRNA expression were higher in 15 deoxyspergualin treated chimeras than in control chimeras , indicating that these cytokines are responsible for an enhancement of hematopoiesis . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Neutralizing antibodies to macrophage colony stimulating factor ( M CSF ) and granulocyte macrophage colony stimulating factor ( GM CSF ) but not antibody to interleukin 3 ( IL 3 ) , or stem cell factor ( SCF ) significantly inhibited the growth of the progenitor cells induced by mesangial cell conditioned media . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Nor is Stat 4 activated in myeloid cells by a number of cytokines , including erythropoietin , IL 3 , granulocyte colony stimulating factor , stem cell factor , colon stimulating factor 1 , hepatocyte growth factor , IL 2 , IL 4 , and IL 6 . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
IL 3 used in conjunction with synergistically acting cytokines such as granulocyte macrophage colony stimulating factor ( GM CSF ) or granulocyte CSF ( G CSF ) have already yielded interesting results , while new factors like stem cell factor are in the process of clinical evaluations and appear promising . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In vitro response of normal and aplastic anemia bone marrow to mast cell growth factor and in combination with granulocyte macrophage colony stimulating factor and interleukin 3 . ^^^ We have examined the effect of mast cell growth factor ( MGF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and interleukin 3 ( IL 3 ) , singly or in combination , on the growth of normal and aplastic anemia ( AA ) bone marrow in clonogenic assay and long term bone marrow culture ( LTBMC ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The FLT3 / FLK2 receptor tyrosine kinase is closely related to two receptors , c Kit and c Fms , which function with their respective ligands , Kit ligand and macrophage colony stimulating factor to control differentiation of haematopoietic and non haematopoietic cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The optimal combination was found to be : Interleukin 3 ( IL 3 ) + Granulocyte Monocyte Colony Stimulating Factor ( GM CSF ) + kit ligand ( KL ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Although these single factors stimulate MTHCs , combinations of these factors with colony stimulating factor 1 ( CSF 1 or M CSF ) or Steel factor ( SLF or SCF ) act synergistically to promote colony formation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
By various analyses , this protein was shown to be molecularly different from the heretofore identified cytokines that may affect megakaryocytopoiesis , i . e . , interleukin 1 ( IL 1 ) , IL 2 , IL 3 , IL 6 , IL 7 , IL 11 , granulocyte colony stimulating factor ( CSF ) , macrophage CSF , GM CSF , leukemia inhibitory factor , stem cell factor and tumor necrosis factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In clonogenetic fetal calf serum supplemented ( FCS+ ) or nonsupplemented ( FCS ) culture treated with saturating levels of interleukin 3 ( IL 3 ) granulocyte macrophage colony stimulating factor ( GM CSF ) and erythropoietin ( Ep ) ( combined with c kit ligand in FCS ( ) culture conditions ) , RA induces a dramatic dose dependent shift from erythroid to granulomonocytic colony formation , the latter colonies being essentially represented by granulocytic clones . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Although cells grown from 5 of 6 AML patients were inhibited by TGF beta 1 , cells from 1 AML patient were growth stimulated by TGF beta 1 in the presence of granulocyte macrophage colony stimulating factor ( GM CSF ) , M CSF , or mast cell growth factor ( kit ligand ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
This process was inhibited by granulocyte and granulocyte / macrophage colony stimulating factor , but not by interleukin 3 ( IL 3 ) , IL 6 , or stem cell factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The cell cycle kinetics of AML blast cells are susceptible to stimulation by a variety of cytokines , including granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin ( IL ) 3 , granulocyte colony stimulating factor and stem cell factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We show here that bone marrow myeloid progenitor cells from p 53 deficient mice are more resistant to induction of apoptosis when there was only a low concentration of the viability factors granulocyte macrophage colony stimulating factor ; interleukins 1 alpha , 3 , and 6 ; or stem cell factor ; or when apoptosis was induced in these cells by irradiation or heat shock . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Studies in which stem cell factor was used in combination with other growth factors showed that stem cell factor allowed the survival of stem cells , while a second growth factor ( granulocyte macrophage colony stimulating factor ) stimulated the stem cells to develop normally . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The viable 5 FU resistant cells were then cultured in semi solid media in the presence of either single cytokines : TCM 5637 , Granulocyte Macrophage Colony Stimulating Factor ( GM CSF ) , or a combination of cytokines : interleukin 1 ( IL 1 ) IL 1 + IL 3 + 5637 , IL 1 + IL 3 + Stem Cell Factor ( SCF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
This is probably an indirect effect inasmuch as these polypeptides can regulate the stromal cell expression of hemopoietic factors such as macrophage colony stimulating factor ( M CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 6 ( IL 6 ) , and the stem cell factor ( SCF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Analysis of a panel of samples from different patients with acute myeloblastic leukemia ( AML ) and myeloid leukemic cell lines indicated that IL 11 alone was ineffective in supporting myeloid leukemic cell growth but frequently enhanced growth supported by IL 3 , granulocyte macrophage colony stimulating factor ( GM CSF ) , or SF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In a kinetic analysis , colony formation by PB granulocyte macrophage colony forming unit ( CFU GM ) was delayed in a dose dependent manner by the addition of activin A only when stimulated with interleukin 3 ( IL 3 ) , but not when stimulated with granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , or stem cell factor ( SCF ) plus G CSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In addition , both lipopolysaccharide and granulocyte macrophage colony stimulating factor induced modest increases in IL 1ra mRNA by cultured SF PMN . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Interleukin 3 , granulocyte macrophage colony stimulating factor and stem cell factor play regulatory functions in the early steps of erythropoiesis . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Polymerase chain reaction analyses showed that most of the cell lines produce FLK 2 / FLT 3 ligand , stem cell factor , macrophage colony stimulating factor , leukemia inhibitory factor , and interleukin 6 ( IL 6 ) , whereas there is a generally low or not measurable production of granulocyte colony stimulating factor , granulocyte macrophage colony stimulating factor , IL 1 , IL 3 , transforming growth factor beta 1 , erythropoietin , or thrombopoietin . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Gradient separated bone marrow mononuclear cells ( BMMNC ) from 38 recipients of TCD BMT , 15 recipients of UM BMT , and 23 normal donors ( NLD ) were plated in cultures of semisolid , serum containing medium with the addition of stem cell factor ( SCF ) , erythropoietin ( Epo ) , and granulocyte colony stimulating factor ( G CSF ) or granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Addition of Steel factor ( SF ) , interleukin 6 ( IL 6 ) , or granulocyte colony stimulating factor ( G CSF ) to cultures containing interleukin 3 enhanced blast cell colony formation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Methylcellulose committed progenitor cell assays were carried out using FL alone and in combinations with granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) and c kit ligand ( KL ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We examined here how DCs differentiated from human cord blood CD34+ progenitor cells cultured with granulocyte macrophage colony stimulating factor , tumor necrosis factor alpha , and stem cell factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We detected erythropoietin ( EPO ) in 11 of 15 , stem cell factor ( SCF ) in 7 of 11 , granulocyte colony stimulating factor ( G CSF ) in 4 of 15 , granulocyte / macrophage colony stimulating factor ( GM CSF ) in 6 of 15 , IL 3 in 1 of 12 , leukemia inhibitory factor ( LIF ) in 1 of 9 , and macrophage colony stimulating factor ( M CSF ) in 1 of 8 conditioned media . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Tumour necrosis factor ( TNF ) alpha exerts multiple effects on human acute myeloblastic leukaemia ( AML ) cells in vitro , including ( 1 ) synergistic stimulation of proliferation with interleukin 3 ( IL 3 ) and granulocyte macrophage colony stimulating factor ( GM CSF ) ; ( 2 ) inhibition of granulocyte CSF ( G CSF ) and stem cell factor ( SCF ) induced growth ; ( 3 ) suppression of multiplication of clonogenic leukaemic cells ; ( 4 ) induction of autocrine growth . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
A marked reduction in the number of blast cell colonies supported by SCF alone was noted by the addition of neutralizing antibody ( Ab ) against granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The recombinant SCF was similar to natural SCF with respect to its interaction with monoclonal antibody and its synergistic biological activity in conjunction with colony stimulating factor . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Murine short term bone marrow cultures were also treated with AcSDKP in the presence or absence of cytokines ( interleukin 3 [ LI 3 ] , stem cell factor [ SCF ] , or granulocyte colony stimulating factor [ G CSF ] ) for various time periods . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Some promising mobilizing regimens include the use of stem cell factor and growth factors such as interleukin 3 , granulocyte colony stimulating factor , and granulocyte macrophage colony stimulating factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
To investigate humoral inhibitory activities we examined the response of 15 DBA bone marrows in vitro to the inhibitory chemokine macrophage inflammatory protein 1 alpha ( MIP 1 alpha ) in the presence of the stimulatory cytokines erythropoietin , granulocyte macrophage colony stimulating factor , IL 3 , and stem cell factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We investigated the effects of granulocyte macrophage colony stimulating factor , interleukin 3 , stem cell factor , interleukin 6 , and granulocyte colony stimulating factor ( G CSF ) alone , and in combination , on the clonogenic potential of normal and aplastic anemia ( AA ) bone marrow mononuclear cells ( BMMC and CD34+ cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Culture of DR cells in a combination of M 2 10B4 derived PG , but not glycoproteins and picogram concentrations of recombinant human interleukin 6 ( IL 6 ) , granulocyte colony stimulating factor ( G CSF ) , stem cell factor ( SCF ) , leukemia inhibitory factor ( LIF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and macrophage inflammatory protein 1alpha ( MIP 1alpha ) resulted in the recovery of 96 % + / 8 % of LTC IC maintained in cultures supplemented with unfractionated stromal SN . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We have studied graft versus host disease ( GVHD ) after transplantation of allogeneic peripheral blood stem cells ( PBSC ) mobilized by either recombinant canine granulocyte colony stimulating factor ( rcG CSF ) alone or combined with stem cell factor ( rcSCF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
FL alone was a weak growth stimulator of CD34+ BM cells , but synergistically and directly enhanced colony formation in combination with interleukin ( IL ) 3 , granulocyte colony stimulating factor ( G CSF ) , CSF 1 , granulocyte macrophage ( GM ) CSF stem cell factor ( SCF ) , and IL 6 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) have been shown to stimulate the circulation of hematopoietic progenitor cells in both mice and nonhuman primates . ^^^ Peripheral blood CD34+ cells differ from bone marrow CD34+ cells in Thy 1 expression and cell cycle status in nonhuman primates mobilized or not mobilized with granulocyte colony stimulating factor and / or stem cell factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The FLT3R belongs to a family of receptor tyrosine kinases involved in hematopoiesis that also includes KIT , the receptor for SCF ( stem cell factor ) , and FMS . the receptor for M CSF ( macrophage colony stimulating factor ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In particular , the following hematopoietic growth factors are discussed : erythropoietin , stem cell factor , interleukin 3 , interleukin 5 , granulocyte macrophage colony stimulating factor , granulocyte colony stimulating factor , and macrophage colony stimulating factor . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Surprisingly , TNF alpha was a very potent stimulator of the proliferation of CD34++CD38 cells and was the most potent synergistic factor for the IL 3 induced proliferation of these cells of all cytokines tested ( IL 1 , IL 6 , granulocyte colony stimulating factor , kit ligand ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In contrast to spontaneous blast proliferation , in the presence of exogenous hematopoietic growth factors ( granulocyte colony stimulating factor , granulocyte macrophage colony stimulating factor , interleukin 3 , tumor necrosis factor alpha , stem cell factor ) , IL 1 inhibition caused either increased or decreased AML blast proliferation depending on the individual patient . ^^^ When AML blasts were cultured with stem cell factor plus granulocyte macrophage colony stimulating factor , IL 1 inhibition significantly increased AML blast proliferation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In contrast , KIT ligand ( KL ) and granulocyte macrophage colony stimulating factor ( GM CSF ) were ineffective in promoting IL 7 dependent pro B cell growth and differentiation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The combinations of cytokines tested that were most efficient in inducing the CD34+ cells into cycle were stem cell factor ( SCF ) plus one of the following : interleukin 1 ( IL 1 ) , IL 3 , granulocyte colony stimulating factor ( G CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The cytokines interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) and stem cell factor ( SCF ) could independently enhance the FL stimulated proliferation in a synergistic fashion . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Co incubation of M 07e with various cytokines and TPO showed additive proliferative effects for interleukin 3 ( IL 3 ) and granulocyte macrophage colony stimulating factor ( GM CSF ) and synergistic responses for stem cell factor ( SCF ) , interferon ( IFN ) alpha , and to a lesser extent for IFN gamma and tumor necrosis factor ( TNF ) alpha . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Moreover , mice receiving either granulocyte colony stimulating factor ( G CSF ) or G CSF+ stem cell factor before low dose TBI ( 160 cGy ) exhibited a marked increase in engraftment compared with mice receiving a vehicle control before low dose TBI ( 18 . 9 % and 20 . 6 % 5 5 . 6 % at a 1 month , respectively ; 29 % and 35 % 5 15 . 1 % at 4 months , respectively ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CD34+ precursors in normal human bone marrow ( BM ) generate large numbers of dendritic cells alongside macrophages and granulocytic precursors when cultured for 12 to 14 days in c kit ligand , granulocyte macrophage colony stimulating factor ( GM CSF ) , and tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We examined the effects of ML , as a single factor or in combinations with early acting factors such as steel factor ( SF ) , interleukin ( IL ) 3 , IL 1 , IL 6 , and granulocyte colony stimulating factor ( G CSF ) , on colony formation from primitive progenitors of mice . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In contrast , stem cell factor ( SCF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and interleukin 3 ( IL 3 ) , as well as G CSF , when tested individually , generated significant numbers of GM colonies in some JCML patients . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Steel factor ( SF ) , interleukin 3 ( IL 3 ) , IL 4 , IL 6 , IL 11 , and granulocyte colony stimulating factor ( G CSF ) as single agents supported formation of significant numbers of colonies , but flt3 / flk 2 ligand ( FL ) and IL 12 did not . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Using a plasma clot clonal assay and a long term bone marrow culture ( LTBMC ) system , we studied the effects of the recently cloned human flt 3 ligand ( FL ) alone and in combination with granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) , or stem cell factor ( c kit ligand [ KL ] ) on human megakaryocytopoiesis . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
B lymphoid potential was not supported , however , in SF + IL 3 or in SF + IL 3 plus any single growth factor ( IL 1 to 12 , granulocyte macrophage colony stimulating factor [ GM CSF ] , G CSF , erythropoietin [ Epo ] , leukemia inhibitory factor [ LIF ] , tumor necrosis factor alpha [ TNF alpha ] , transforming growth factor beta [ TGF beta ] , gamma interferon [ IFN gamma ] , or insulin like growth factor 1 [ IGF 1 ] ) , but was supported in SF + IL 3 + 5 % PWM SCM . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Effect of cytokine treatment ( granulocyte colony stimulating factor and stem cell factor ) on hematopoiesis and the circulating pool of hematopoietic stem cells in mice . ^^^ The mobilization of hematopoietic stem cells ( HSCs ) into the peripheral blood of mice was induced by recombinant human granulocyte colony stimulating factor ( rhG CSF ) ( 250 microgram / kg / d ) alone or combined with recombinant rat stem cell factor ( rrSCF ) ( 34 microgram / kg / d ) , injected subcutaneously ( s . c . ) once a day for 10 and 17 days . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) and transforming growth factor beta 1 ( TGF beta l ) potently inhibited the clonal growth of murine Lin Sca l+ bone marrow progenitors stimulated by FL alone or in combination with granulocyte colony stimulating factor ( G CSF ) , stem cell factor ( SCF ) , interleukin ( IL ) 3 , IL 6 , IL 11 , or IL 12 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In addition , individual cells in this population can differentiate either to natural killer cells in the presence of stem cell factor ( SCF ) , interleukin 7 ( IL 7 ) , and IL 2 or to dendritic cells in the presence of SCF , granulocyte macrophage colony stimulating factor , and tumor necrosis factor alpha ( TNFalpha ) , indicating that CD34+CD38dim thymocytes contain multi potential hematopoietic progenitors . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Quantitative reverse transcriptase polymerase chain reaction analysis revealed that the mRNAs of macrophage colony stimulating factor and stem cell factor , but not that of Interleukin 3 , were detectable in all the hematopoietic supportive and non supportive stromal cell lines . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Incubation with single recombinant cytokines from a broad spectrum of growth factors established that the strongest proliferation response of MUTZ 2 cells was elicited by FLT 3 ligand , granulocyte colony stimulating factor ( G CSF ) , macrophage CSF ( M CSF ) , interferon gamma ( IFN gamma ) and stem cell factor ( SCF ) , whereas granulocyte macrophage CSF ( GM CSF ) , M CSF , interleukin 3 ( IL 3 ) and SCF were the most effective growth factors in inducing proliferation of MUTZ 3 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
When combined with SF , TPO showed stronger stimulation of production of progenitors in suspension culture than other early acting factors , such as IL 6 , IL 11 , and granulocyte colony stimulating factor ( G CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We have previously shown that normal CD34+ HPCs express high levels of fibronectin ( Fn ) receptors very late antigen 4 ( VLA 4 ) and VLA 5 in a low affinity state , which do not allow HPCs to strongly adhere on immobilized Fn , and that cytokines such as interleukin 3 , granulocyte monocyte colony stimulating factor , and stem cell factor transiently activate these receptors , providing HPCs with an adhesive phenotype on Fn . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
IL1RA release from AML blasts was also increased by stem cell factor , tumour necrosis factor alpha ( TNF alpha ) , granulocyte macrophage colony stimulating factor and macrophage colony stimulating factor , whereas interleukin 3 , interleukin 6 , leukaemia inhibitory factor and granulocyte colony stimulating factor did not significantly alter IL1RA release . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Also , none of these compounds affected SCF synergism with other hematopoietic growth factors , such as interleukin 3 or granulocyte colony stimulating factor , suggesting a population specific phenomenon . ^^^ Reactive oxygen species mediate stem cell factor synergy with granulocyte / macrophage colony stimulating factor in a subpopulation of primitive murine hematopoietic progenitor cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We have examined the repopulating ability of bone marrow and peripheral blood cells collected immediately and at intervals after treatment of donor mice with the combination of granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) . ^^^ Bone marrow collected 14 days after in vivo administration of granulocyte colony stimulating factor and stem cell factor to mice has 10 fold more repopulating ability than untreated bone marrow . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Monocytes from SF and blood of patients with RA produced highly elevated levels of interleukin 1 alpha ( IL 1 alpha ) , IL 6 , tumor necrosis factor alpha ( TNF alpha ) , and granulocyte macrophage colony stimulating factor ( GMCSF ) , the leading mediators of inflammation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Transcripts for interleukin ( IL ) 6 , IL 7 , macrophage colony stimulating factor ( M CSF ) , and stem cell factor ( SCF ) were detected in FS 1 cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
It has been demonstrated that these cells produce RNA corresponding to the interleukins IL 1 alpha , 1 beta , 3 , 7 , 8 , and 11 , granulocyte macrophage colony stimulating factor ( GM CSF ) , stem cell factor ( SCF ) , transforming growth factor beta ( TGF beta ) , tumor necrosis factor alpha ( TNF alpha ) , interferon alpha ( INF alpha ) , and basic fibroblast growth factor ( bFGF ) . ^^^ Additionally , RNA corresponding to the receptors for IL 6 , GM CSF , SCF , INF alpha , beta , bFGF , and monocyte colony stimulating factor ( M CSF ) were also expressed by the cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
MTK 1 also inhibited the bone marrow derived colony forming unit granulocyte / macrophage ( CFU GM ) formed by granulocyte macrophage colony stimulating factor ( GM CSF ) and SCF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Human erythroid progenitor cells were isolated from peripheral blood of healthy donors and amplified in a suspension culture system using recombinant growth factors ( stem cell factor , interleukin 3 , granulocyte macrophage colony stimulating factor and erythropoietin ) as well as conditioned medium from a human bone marrow stroma cell line to support cell proliferation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Granulocyte / macrophage colony stimulating factor and kit ligand cotreatment did not overcome this inhibition . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The purified CD34+ cells were cultured for 14 days with various combinations of cytokines , including recombinant human macrophage colony stimulating factor ( rM CSF ) , granulocyte CSF ( rG CSF ) , granulocyte macrophage CSF ( rGM CSF ) , interleukin 3 ( rIL 3 ) , and stem cell factor ( SCF ; a ligand for c kit ) in serum free medium . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Both preclinical and early clinical studies using recombinant methionyl human SCF plus recombinant methionyl human granulocyte colony stimulating factor ( Filgrastim ) have demonstrated increased PBPC mobilization as compared with the use of either factor alone . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Although c fms is homologous to c kit , which encodes the receptor for stem cell factor ( SCF ) , EML C 1 cells neither express c fms nor respond to macrophage colony stimulating factor ( M CSF ) , the ligand for c fms . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Preincubation of the cells with MGF in suspension did not sensitize them to the effect of IL 3 , granulocyte macrophage colony stimulating factor ( GM CSF ) , G CSF , or IL 4 in a clonogenic cell culture assay . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The level of alkyltransferase in CD34+ cells or in mononuclear BM cells did not increase after incubation with granulocyte macrophage colony stimulating factor , interleukin 3 , stem cell factor , the combination , or 5637 conditioned medium . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Continuous culture of these hematopoietic cells in the presence of the cytokines interleukin 3 , c kit ligand , granulocyte colony stimulating factor resulted in tenfold expansion by day 7 and 25 fold expansion by day 14 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Combinations of cytokines , such as kit ligand ( KL ) + interleukin 3 or KL + granulocyte macrophage colony stimulating factor ( GM CSF ) upregulated the expression of CD 95 on PHPs upon in vitro culture . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
A clinical isolate of HCMV inhibited the colony formation of myeloid BM progenitors responsive to granulocyte macrophage colony stimulating factor ( CSF ) , granulocyte CSF , macrophage CSF , interleukin 3 ( IL 3 ) and the combination of IL 3 and stem cell factor ( SCF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Here , we show that the sequential use of early acting hematopoietic growth factors , stem cell factor , interleukin ( IL ) 3 , and IL 6 , followed on day 8 by differentiation in the two factor combination IL 4 plus granulocytemacrophage colony stimulating factor ( GM CSF ) ( CC4GM ) is more efficient and allows the cells to be arrested in the LC stage for more than 1 week while continuous maturation occurs in CC 7 7 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Substantial expansion of myeloid ( 181 fold ) and megakaryocyte ( 41 fold ) progenitors cells was demonstrated , using the conditions that we determined to be optimal : CD34+ progenitors cultured unperturbed for 7 ( marrow ) or 10 ( blood ) days in Teflon coated bags with 10 Vivo 10 medium containing 10 % autologous plasma , 100 ng / ml , respectively , of recombinant stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , interleukin 6 ( IL 6 ) , and granulocyte colony stimulating factor ( G CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We compared the in vitro response of myeloid progenitor cells [ colony forming units of culture ( CFU C ) ] that were prepared from human umbilical cord blood to the administration of the combination of granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) versus that of CFU C obtained from normal human bone marrow . ^^^ Synergistic response of cord blood myeloid progenitor cells to the combined administration of human granulocyte colony stimulating factor and human stem cell factor in vitro . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The ability of Tpo to maintain viable Lin Sca 1+ progenitors was comparable to that of granulocyte colony stimulating factor and interleukin 1 , whereas stem cell factor ( SCF ) promoted the viability of a higher number of Lin Sca 1+ progenitor cells when incubated for 40 hours . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
A number of factors possessing burst promoting activity ( BPA ) have been characterized , such as interleukin 3 ( IL 3 ) , stem cell factor ( SCF ) , and granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CD34+ cells isolated from bone marrow were cultured in iscove ' s modified Dulbecco ' s medium supplemented with 10 % fetal calf serum , 1 % bovine serum albumin , 50 ng / mL stem cell factor , 50 ng / mL interleukin 3 ( IL 3 ) , 50 ng / mL IL 6 , 100 ng / mL granulocyte colony stimulating factor , and 3 U / mL erythropoietin for 7 days . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In previous studies we showed that 5 days of treatment with granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) mobilized murine repopulating cells to the peripheral blood ( PB ) and that these cells could be efficiently transduced with retroviral vectors . ^^^ Improved retroviral gene transfer into murine and Rhesus peripheral blood or bone marrow repopulating cells primed in vivo with stem cell factor and granulocyte colony stimulating factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
IL 1 beta , granulocyte macrophage colony stimulating factor , macrophage colony stimulating factor and stem cell factor increased IL 6 secretion , whereas IL 4 caused decreased IL 6 secretion together with increased release of IL 1 receptor antagonist . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Similar results were obtained in culture supplemented with a combination of IL 3 , IL 6 , SCF , granulocyte colony stimulating factor , erythropoietin , and thrombopoietin . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
When selected PB CD34+ cells that were expanded ex vivo with combinations of human hematopoietic growth factors including the c kit ligand ( KL ) , interleukin ( IL ) 1 beta , IL 3 , IL 6 , erythropoietin ( EPO ) and granulocyte macrophage colony stimulating factor ( GM CSF ) for 14 days were grafted to cotransplant carrying SCID mice , a considerable loss of their proliferative potential was observed regardless of the HGF combination used . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CD34+ cells were cultured in semisolid medium containing serum with increasing concentrations of GM CSF , G CSF , macrophage colony stimulating factor ( M CSF ) , stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) and IL 11 alone or in combination with HGF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The effects of interleukins 3 and 6 , stem cell factor , and granulocyte macrophage colony stimulating factor on human fetal hematopoietic , bone marrow , and cord blood cells were studied on the basis of the colony forming capacity . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
By assessing the formation of colonies , CD34+ enrichment was evaluated with and without cytokine stimulation ( interleukins 3 and 6 , stem cell factor , granulocyte macrophage colony stimulating factor ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We have investigated the mobilization of these cells into the blood of patients with ovarian carcinoma randomized to receive granulocyte colony stimulating factor ( G CSF ; 5 micrograms / kg ) plus different doses of stem cell factor ( SCF ; c kit ligand ) after chemotherapy or G CSF alone after chemotherapy . ^^^ Increased numbers of long term culture initiating cells in the apheresis product of patients randomized to receive increasing doses of stem cell factor administered in combination with chemotherapy and a standard dose of granulocyte colony stimulating factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We have performed a comprehensive analysis of cell lines and tissues to compare and contrast the expression patterns of Flt 3 ligand ( FL ) , c Kit ligand ( KL ) , and macrophage colony stimulating factor as well as their receptors , Flt 3 , c Kit , and c Fms . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In contrast , stem cell factor ( SCF ) , granulocyte / macrophage colony stimulating factor ( GM CSF ) , IL 10 , IL 8 , monocyte chemotactic and activating factor ( MCAF ) , and the complement split product C5a failed to modulate the expression of any adhesion molecule examined . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Male donor mice ( age 8 12 weeks , weight 20 25 grams ) were pretreated intraperitoneally ( ip ) with a fixed dose of 2 . 5 micrograms of either granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , IL 3 , stem cell factor ( SCF ) , or saline administered twice daily for 2 to 4 days . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
On the other hand , murine M 2 10B4 fibroblasts engineered to produce high levels of both human granulocyte colony stimulating factor ( G CSF ) and interleukin 3 ( IL 3 ; 190 and 4 ng / mL , respectively ) , either alone or mixed 1 : 1 with SI / SI fibroblasts engineered to produce high levels of soluble Steel factor ( SF ) , with or without production of the transmembrane form of SF ( 60 and 4 ng / mL , respectively ) , stimulated the production of up to 20 fold more CFC in LTC of cells from normal human marrow , G CSF mobilized blood or cord blood when compared with parallel cocultures containing HMF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Mononuclear BM cells from patients with newly diagnosed AML were cultured in serum free conditions with flt 3 L , SCF , granulocyte colony stimulating factor ( G CSF ) and granulocyte macrophage colony stimulating factor ( GM CSF ) alone and in combination . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Nonadherent mononuclear bone marrow cells were incubated for 8 days in the presence of the serum and recombinant canine hemopoietic cytokines ( stem cell factor , granulocyte colony stimulating factor , GM colony stimulating factor ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CD1a+ dendritic cells ( DC ) differentiate from a major population of nonadherent CD 13 ( hi ) lin cells that appear when human cord blood CD34+ hematopoietic progenitor cells are cultured with stem cell factor , granulocyte / macrophage ( MA ) colony stimulating factor , and tumor necrosis factor alpha ( TNF alpha ) for 5 days . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We investigated the recovery of human myeloid progenitors incubated with IL 11 alone or in combination with other cytokines , including stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) and granulocyte macrophage colony stimulating factor ( GM CSF ) following their in vitro treatment with ARA C ( 10 ( 9 ) M ) or Eilatin ( 10 ( 7 ) M ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The supernatants of primary cell and BMEC 1 contain stem cell factor , interleukin 6 ( IL 6 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , IL 1 alpha , IL 11 , and G CSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
After retroviral transduction , human CD34+ cells were differentiated into DCs in vitro using granulocyte macrophage colony stimulating factor , tumor necrosis factor alpha , and stem cell factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We found that a combination of epidermal growth factor ( EGF ) , the OP 9 stromal cell line , which lacks macrophage colony stimulating factor , recombinant stem cell factor , and the chemically defined medium mSFO 2 provides a microenvironment where c Kit+ Thy 1+ / lo Mac 1+ / lo B 220 TER 119 common beta + IL 2R gamma + gp130+ cells are selectively propagated from normal , unfractionated bone marrow cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Retroviral transduction of human progenitor cells : use of granulocyte colony stimulating factor plus stem cell factor to mobilize progenitor cells in vivo and stimulation by Flt3 / Flk 2 ligand in vitro . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Among recombinant cytokines , interleukin 3 ( IL 3 ) , IL 6 , and IL 7 , but not IL 2 , IL 4 , stem cell factor ( SCF ) , and granulocyte macrophage colony stimulating factor ( GM CSF ) could induce RAG 1 activation in FL8 . 2 . 4 . 4 cells , and a significant synergistic effect between IL 3 , IL 6 , and IL 7 was observed . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The effect of macrophage colony stimulating factor ( M CSF ) and stem cell factor ( SCF ) on osteoclast progenitors was examined by pre culturing mouse bone marrow ( BM ) cells in agar medium containing M CSF or SCF and overlaying CESJ medium 0 7 days later . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The addition of granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage CSF ( GM CSF ) , IL 1beta , IL 11 or macrophage inflammatory protein 1alpha to cultures containing IL 3 , SCF and IL 6 could not explain the SCM effect and in all these combinations SCM addition further increased the recovery of HSC subsets . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In the present work , mouse spleen cells were cultured with recombinant ( r ) IL 1 beta , rIL 5 , rIL 6 , rIL 9 , granulocyte macrophage colony stimulating factor ( GM CSF ) , stem cell factor ( SCF ) , tumor transforming growth factor beta ( TGF beta ) , or tumor necrosis factor alpha ( TNF alpha ) in the presence of endogenous IL 3 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
A combination of erythropoietin ( EPO ) plus stem cell factor ( SCF ) drove purified unfractionated granulocyte colony stimulating factor ( G CSF ) / chemotherapy mobilized peripheral blood CD34+ cells to selective erythroid differentiation in liquid culture with an average 28 fold increase in the total cell number after 21 d . ^^^ In parallel experiments the same circulating CD34+ cells underwent granulocytic / monocytic differentiation in liquid culture in response to granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) and SCF , demonstrating that these CD34+ progenitors had intact pluripotent differentiating potential . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The myeloid cytokines granulocyte colony stimulating factor , granulocyte monocyte colony stimulating factor , interleukin 3 and stem cell factor enhanced the adhesion of AML blast cells to BMFs in some cases . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In a methylcellulose system , where a combination of stem cell factor ( SCF ) , IL 3 , and granulocyte / macrophage colony stimulating factor ( GM CSF ) were used , TSP inhibited the growth of colony forming unit granulocyte erythroblast , megakaryocyte , and macrophage ( CFU GEMM ) but not CFU GM and burst forming unit erythroblast ( BFU E ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Using this approach distinct expression patterns have been shown for the receptors for interleukin 3 ( IL 3 ) , IL 6 , granulocyte / macrophage colony stimulating factor ( GM CSF ) and Steel Factor ( SF ) on subsets of CD34+ and CD 34 cells in bone marrow . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We conclude that long term marrow repopulating cells mobilized into circulation by treatment with granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) are capable of maintaining lifelong polyclonal hematopoiesis in reconstituted mice . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Moreover , we found that CD 44 mediated adhesion of CD34+ cells to HA could be enhanced by phorbol 12 myristate 13 acetate ( PMA ) , the function activating anti CD 44 monoclonal antibody H 90 , and cytokines such as granulocyte monocyte colony stimulating factor , interleukin 3 ( IL 3 ) , and stem cell factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The growth factors granulocyte colony stimulating factor ( G CSF ) and erythropoietin ( EPO ) , but not interleukin 3 ( IL 3 ) or stem cell factor ( SCF ) , also stimulated platelet activation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The unstimulated and induced production of granulocyte macrophage colony stimulating factor ( GM CSF ) , granulocyte colony stimulating factor ( G CSF ) , IL 3 , IL 6 , stem cell factor ( SCF ) , IL 1beta , tumour necrosis factor alpha ( TNF alpha ) , TNF beta , interferon gamma ( IFN gamma ) and transforming growth factor beta ( TGF beta ) was determined after culture of blood mononuclear cells from 22 patients with severe beta thalassaemia in a regular transfusion programme , five non regularly transfused patients with beta thalassaemia intermedia and nine normal persons . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We studied the interaction of interleukin ( IL ) 4 and other burst promoting activity ( BPA ) factors , such as IL 3 , granulocyte / macrophage colony stimulating factor ( GM CSF ) , IL 9 and stem cell factor ( SCF ) , on erythroid burst forming unit ( BFU E ) and erythrocyte containing mixed ( CFU Mix ) colony formation in serum free culture . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
MGDF also enhanced interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , c kit ligand ( KL ) and FLT 3 ligand ( FL ) stimulated blast cell proliferation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Costimulatory effects of granulocyte colony stimulating factor ( G CSF ) , granulocyte / macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) and stem cell factor ( SCF ) on the generation of lymphokine activated killer ( LAK ) , bacillus Calmette Gurin activated killer ( BAK ) and natural killer ( NK ) cell cytotoxicity were investigated in this assay . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Furthermore , the TPO induced proliferation of AML cells was augmented by the addition of the other hematopoietic growth factors such as interleukin 3 ( IL 3 ) , IL 6 , stem cell factor , or granulocyte macrophage colony stimulating factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Previous studies have shown that primitive human hematopoietic cells detectable as long term culture initiating cells ( LTC ICs ) and colony forming cells ( CFCs ) can be amplified when CD 34 ( + ) CD 38 ( ) marrow cells are cultured for 10 days in serum free medium containing flt 3 ligand ( FL ) , Steel factor ( SF ) , interleukin ( IL ) 3 , IL 6 , and granulocyte colony stimulating factor . ^^^ Thus , maximal expansion of LTC ICs ( 60 fold ) was obtained in the presence of 30 times more FL , SF , IL 3 , IL 6 , and granulocyte colony stimulating factor than could concomitantly stimulate the near maximal ( 280 fold ) amplification of CFCs . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We show that a five factor combination of interleukin 3 ( IL 3 ) , IL 6 , granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage CSF ( GM CSF ) , and stem cell factor ( SCF ) produces a mean increase of 85 % in colony number . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The enriched population of CD34+ cells ( more than 95 % ) was cultured in the presence of stem cell factor , interleukin 3 ( IL 3 ) , IL 6 , and either granulocyte colony stimulating factor or macrophage colony stimulating factor to achieve granulocyte or monocyte / macrophage differentiation , respectively . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Activation of ToMC by recombinant human ( rh ) SCF or anti IgE resulted in histamine secretion , whereas no effects were seen with rhC5a , rh granulocyte macrophage colony stimulating factor , or rh interleukin 1 through 10 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CD34+ cord blood ( CB ) cells were expanded in stromal cell free long term culture ( LTC ) , in the presence of various combinations of interleukin 3 ( IL 3 ) , stem cell factor ( SCF ) , IL 6 , granulocyte macrophage colony stimulating factor ( GM CSF ) , and anti transforming growth factor beta ( anti TGF beta ) antibody . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Ex vivo expansion cultures were established in Teflon cell culture bags ( American Fluoroseal Corp , Columbia , MD ) in 25 ml defined medium ( Amgen Inc , Thousand Oaks , CA ) containing 100 ng / ml each of stem cell factor ( SCF ) , granulocyte colony stimulating factor ( G CSF ) , and megakaryocyte growth and development factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The expansions of nucleated cells , granulocyte macrophage colony stimulating factor ( GM CSF ) responsive committed precursors , IL 1 + IL 3 + SCF + erythropoietin ( EPO ) responsive multilineage progenitors , and 5 fluorouracil ( 5 FU ) resistant quiescent progenitor were 8 fold , 59 fold , 4 . 4 fold , and 2 . 2 fold , respectively . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
While housekeeping genes were expressed normally in BMSC of infected mice , the expression of genes encoding the essential hemopoietins SCF , granulocyte colony stimulating factor , and interleukin 6 was markedly reduced . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The combinations of GF used sustained either multilineage [ stem cell factor ( SCF ) + interleukin 3 ( IL 3 ) , or erythroid [ SCF + IL 3 + erythropoietin ( Epo ) ] or myeloid [ SCF + IL 3 + granulocyte colony stimulating factor ( G CSF ) ] differentiation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We showed that even a complex cocktail of HGFs ( stem cell factor , interleukin 3 , IL 6 , IL 11 , granulocyte colony stimulating factor , and erythropoietin ) is unable to induce significant myeloid differentiation in day 12 embryoid bodies . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
SCF also potentiates the mobilization of hematopoietic progenitor cells into the peripheral blood in response to granulocyte colony stimulating factor ( G CSF ) , with or without chemotherapy . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The inhibitory effect of IL 6 on blast cell colony formation was retained when IL 6 was combined with granulocyte colony stimulating factor , but was lost if IL 6 was used in combination with mast cell growth factor , IL 3 , granulocyte macrophage colony stimulating factor , or IL 4 . ^^^ In these cases , however , IL 6 stimulated significantly clonogenic cell growth if combined with mast cell growth factor or granulocyte macrophage colony stimulating factor . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In methylcellulose cultures , flt3L stimulated formation of hematopoietic colonies only weakly , whereas it had an additive effect when combined with erythropoietin ( Epo ) , stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , interleukin 11 ( IL 11 ) , or granulocyte colony stimulating factor ( G CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CD34+ cells were cultured in liquid medium in the presence of interleukin 3 , granulocyte macrophage colony stimulating factor . stem cell factor , granulocyte colony stimulating factor and a powerful proliferation with prevalent differentiation along the granulocytic / monocytic lineage was obtained . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Recombinant human granulocyte macrophage colony stimulating factor ( CSF ) , but not recombinant human granulocyte CSF , down regulates the recombinant human stem cell factor dependent differentiation of human fetal liver derived mast cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Neither , stem cell factor ( SCF ) nor granulocyte colony stimulating factor ( G CSF ) alone supported the growth of SAS 1 cells , but supported their viability for more than 4 days and arrested them in the G0 / G1 phase . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Previous reports demonstrating expression of Hyl and Matk in hematopoietic lineages led us to investigate the regulation of Lsk expression in response to stem cell factor ( SCF ) and granulocyte macrophage colony stimulating factor ( GM CSF ) in M07e , a human leukemic cell line . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Using suitable assay systems , we analyzed cytokines in AGL SCM and found significant increases in stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and IL 6 levels compared with control SCM . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Whereas secretion of cytokines lacking a transmembrane domain precursor ( IL 1 alpha , IL 1 beta , IL 6 or IL 10 ) is either unaffected or augmented , shedding / secretion of transmembrane domain containing cytokines and cytokine receptors [ TNF alpha , macrophage colony stimulating factor ( M CSF ) , transforming growth factor ( TGF ) alpha , stem cell factor ( SCF ) , TNF R 55 , TNF R 75 , and IL 6R ] was dramatically decreased in the presence of the metalloproteinase inhibitor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Delayed addition of a cytokine cocktail ( cytokines ; interleukin ( IL ) 3 , IL 6 , stem cell factor , erythropoietin , granulocyte macrophage colony stimulating factor , and TPO ) to cultures with TPO alone on day 7 induced various colonies including granulocyte macrophage ( GM ) colonies , erythroid bursts ( E ) , granulocyte erythrocyte macrophage megakaryocyte ( GEMM ) colonies . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The addition of FL to serum free cultures of CD34+ cells supplemented with TGF beta 1 , granulocyte macrophage colony stimulating factor , tumor necrosis factor alpha , and stem cell factor strongly increases both percentages ( mean , 36 % + / 5 % 5 64 % + / 4 % ; P = . 001 ) and total numbers ( 4 . 4 + / 0 . 8 fold ) of CD1a+ dendritic cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The level of cell adhesion was significantly reduced by low concentrations of interleukin 3 ( IL 3 ) ( 2 . 5 to 10 ng / mL ) , whereas stem cell factor ( SCF ) and granulocyte colony stimulating factor ( G CSF ) at these concentrations did not affect adherence of the cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Recipients were injected at 1 2 years of age with rat stem cell factor ( SCF ) and human granulocyte colony stimulating factor ( G CSF ) for 7 days and tested for donor cells in the blood . ^^^ Twenty seven of 49 animals were negative in the blood , liver , and spleen before cytokine therapy ; 14 of 27 ( 52 % ) became positive in the blood after stem cell factor / granulocyte colony stimulating factor injection . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In an increasing number of hematopoietic cytokine receptor systems ( T cell receptor , B cell receptor , and macrophage colony stimulating factor , stem cell factor , interleukin 3 , and erythropoietin [ EPO ] receptors ) , inhibitory roles for the protein tyrosine phosphatase hematopoietic cell phosphatase ( HCP ; SHPTP 1 , PTP1C , and SHP 1 ) have been defined in proliferative signaling . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
To characterize the expression of wt 1 in differentiating hematopoietic progenitors , we isolated and cultured CD34+ progenitor cells from four healthy bone marrow donors with stem cell factor ( SCF ) and granulocyte colony stimulating factor ( G CSF ) to induce differentiation into granulocytes . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Besides expressing stem cell factor ( SCF ) and macrophage colony stimulating factor ( M CSF ) , PA 6 cells were found by flow cytometry analysis to express high levels of stem cell antigen 1 ( Sca 1 ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The addition of stem cell factor ( SCF ) , but not of IL 6 , IL 11 , granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage ( GM ) CSF , IL 1 , and IL 7 , increased CFC and LTC IC expansion beyond the levels observed with SCM+IL 3 alone . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The ligand for flt 3 ( FLT3L ) exhibits striking structural homology with stem cell factor ( SCF ) and monocyte colony stimulating factor ( M CSF ) and also acts in synergy with a range of other hematopoietic growth factors ( HGF ) . ^^^ To investigate the basis for the capacity of FLT3L to augment the de novo generation of myeloid progenitors from CD34+CD38 cells , single bone marrow CD34+CD38 cells were sorted into Terasaki wells containing serum free medium supplemented with interleukin 3 ( IL 3 ) , IL 6 , granulocyte colony stimulating factor ( G CSF ) , SCF ( 4 HGF ) + / FLT3L . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Significantly , soluble growth factors such as granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) or SCF of MS 5 stromal cells ( MS 5 CM ) could replace the requirement of stroma cells for a considerable period . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Selected CD34+ cells from mobilized apheresis products were cultured in serum free or serum containing media supplemented with granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) , and stem cell factor ( SCF ; c kit ligand ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Hematopoietic stem cells , CD 34 positive cells , were isolated from the peripheral blood of three patients with malignant lymphoma , and were cultivated in suspension for 14 days in the presence of cytokines , including granulocyte colony stimulating factor , granulocyte macrophage colony stimulating factor , interleukin 3 and stem cell factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Here we examined the effect of combining stem cell factor ( SCF ) and granulocyte colony stimulating factor ( G CSF ) versus G CSF alone in a randomized clinical study involving 62 women with early stage breast cancer . ^^^ As with administration of G CSF alone , the combination of SCF plus G CSF did not alter the wide variation in levels of PBPC observed between individuals and did not alter the selective nature of PBPC release , with preferential release of day 14 granulocyte macrophage colony stimulating factor ( GM CFC ) versus day 7 GM CFC . ^^^ Enhanced levels and enhanced clonogenic capacity of blood progenitor cells following administration of stem cell factor plus granulocyte colony stimulating factor to humans . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Migration depended on the continuous presence of hematopoietic cytokines interleukin 3 ( IL 3 ) , granulocyte colony stimulating factor ( G CSF ) , macrophage CSF ( M CSF ) , granulocyte macrophage CSF ( GM CSF ) , or stem cell factor ( SCF ) , whereas other cytokines , such as IL 8 , macrophage inflammatory protein 1alpha , macrophage chemotactic and activating factor , and erythropoietin resulted in very little or no migratory response . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Results obtained with SCF treatment were similar to those previously obtained in the same model with recombinant human granulocyte colony stimulating factor ( rhG CSF ) treatment except that recovery of lymphocytes to 500 / mm3 appeared to be more rapid in G CSF treated dogs ( median 15 days vs . 21 days , p = 0 . 03 ) . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Our previous studies indicated that maximal production of nucleated cells and myeloid progenitor cells from PB CD34+ cells occurred with multiple hematopoietic growth factor ( HGF ) , notably the 6 HGF combination of interleukin ( IL ) 1 , IL 3 , IL 6 , granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage CSF ( GM CSF ) , and stem cell factor ( SCF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Low dose stem cell factor , 25 micrograms / kg / d , that does not elicit a detectable biological response , enhances the effects of granulocyte colony stimulating factor in vivo , increasing the neutrophilia and circulation of progenitor and marrow repopulating cells above that which is achieved with either factor alone . ^^^ Phase 1 2 trials of low dose stem cell factor in combination with granulocyte colony stimulating factor show that the combination increases the circulation of CD34+ cells and colony forming progenitor cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Other members of this family include Steel factor ( also known as mast cell growth factor , stem cell factor , and kit ligand ) and colony stimulating factor 1 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Non adherent mononuclear bone marrow cells were incubated in the presence of the serum and granulocyte / macrophage colony stimulating factor ( GM CSF ) , granulocyte stimulating factor ( G CSF ) , or stem cell factor ( SCF ) , and the number of GM CFU counted . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We report the generation of dendritic cells ( DC ) starting from CD34+ bone marrow ( BM ) progenitor cells , using a two stage culture system in which , besides granulocyte macrophage colony stimulating factor ( GM CSF ) and tumour necrosis factor alpha ( TNF alpha ) , stem cell factor ( SCF ) was added during the first 5 days , while interleukin 4 ( IL 4 ) and / or interferon gamma ( IFN gamma ) were added during the secondary culture period of 9 days . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We first evaluated the proliferative response of 3 leukemic cell lines and 32 primary samples from AML patients to IL 9 alone and combined with rh IL 3 , granulocyte macrophage colony stimulating factor ( GM CSF ) and stem cell factor ( SCF , c kit ligand ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Moreover , colony formation ( including CFU G , BFU E , CFU Mix , and CFU Meg ) was observed even in the presence of neutralizing antibodies for granulocyte colony stimulating factor , erythropoietin , and thrombopoietin ( c Mpl ) , whereas neutralizing antibodies for gp 130 , IL 6R , IL 3 , and SCF partially or completely blocked the synergistic effect . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We found that anti VLA 4 treatment combined with either granulocyte colony stimulating factor ( G CSF ) treatment or kit ligand treatment leads to significant enhancement of mobilization efficiency ( fivefold to eightfold ) well above the levels produced by either cytokine alone or anti VLA 4 treatment alone . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Upon culture with granulocyte macrophage colony stimulating factor ( GM CSF ) and stem cell factor ( SCF ) for 14 days , wild type mouse Lin c kit+ HPCs did not exhibit characteristic features of DC such as sheet like projections and veil processes . ^^^ Induction of dendritic cell differentiation by granulocyte macrophage colony stimulating factor , stem cell factor , and tumor necrosis factor alpha in vitro from lineage phenotypes negative c kit+ murine hematopoietic progenitor cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Culturing CD34+ cells with stem cell factor ( c kit ligand ) plus granulocyte macrophage colony stimulating factor resulted in the appearance of a significant proportion of cells expressing phenotypic markers characteristic of dendritic cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Cytokines that were upregulated included interleukin 1alpha ( IL 1alpha ) and 1beta , IL 1 receptor antagonist , IL 6 , IL 11 , granulocyte colony stimulating factor , granulocyte macrophage colony stimulating factor , macrophage colony stimulating factor , stem cell factor , lymphotoxin beta , transforming growth factor beta 1 , and tumor necrosis factor alpha . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
To analyze the synergism between SCF and granulocyte macrophage colony stimulating factor ( GM CSF ) , we established a new megakaryoblastic cell line , HML 2 , by culture in the presence of both SCF and GM CSF . ^^^ Analysis of synergism between stem cell factor and granulocyte macrophage colony stimulating factor on human megakaryoblastic cells : an increase in tyrosine phosphorylation of 145 kDa subunit of c kit in two factor combination . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The response of human acute myelogenous leukemia ( AML ) cells to four different hematopoietic growth factors ( granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 1 beta ( IL 1beta ) , interleukin 3 ( IL 3 ) , and stem cell factor ( SCF ) ) and the relationship of the proliferative response of the AML cells to treatment outcome were studied . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Cells from all three sources that had been expanded ex vivo in the presence of stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , IL 6 , and granulocyte colony stimulating factor ( G CSF ) showed transduction efficiencies ranging from 5 45 % , as measured by acquisition of G 418 resistance . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CD34+ cells were cultured in the presence of EPO , SCF , interleukin 3 , granulocyte / monocyte colony stimulating factor and granulocyte colony stimulating factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
ELISA documented high amounts of granulocyte colony stimulating factor ( G CSF ; > 5 ng / ml ) ; also granulocyte macrophage CSF ( GM CSF ) , macrophage CSF ( M CSF ) , stem cell factor ( SCF ) , IL 6 , and IL 8 were detected in KU 19 19 CM . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
RESULTS : All of these cell lines express granulocyte macrophage colony stimulating factor ( GM CSF ) and macrophage colony stimulating factor ( M CSF ) , and the DU 145 and PC 3 lines express stem cell factor ( SCF ) , as determined by RT PCR and ELISA . ^^^ Each of these cell lines expresses the receptors for SCF , GM CSF , M CSF , and granulocyte colony stimulating factor ( G CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
There were no differences as to protein production of the extracellular matrix , production of granulocytic macrophagal colony stimulating factor , stem cell factor , transforming growth factor beta , interleukin 1 beta and interleukin 6 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Eotaxin activity synergized with stem cell factor but not with interleukin 3 or granulocyte macrophage colony stimulating factor and was inhibited by pertussis toxin . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The system sustained extensive erythropoiesis at low erythropoietin concentrations ( 0 . 2 U / mL ) , plus stem cell factor , interleukin 3 , granulocyte macrophage colony stimulating factor , and insulin like growth factor 1 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
GM CFC will form neutrophils in the presence of the cytokines stem cell factor and granulocyte colony stimulating factor , whereas macrophage colony stimulating factor leads to macrophage formation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
IL 1 alpha , IL 1 beta , and IL 3 , tumour necrosis factor alpha ( TNF alpha ) , Stem cell factor ( SCF ) and granulocyte macrophage colony stimulating factor ( GM CSF ) ( at 10 ng / ml ) stimulated IL 6 and IL 8 production in 0 % and 10 % FCS . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Culture of these isolated CD 34 ( + ) CD 33 ( lo ) thymic progenitors with a mixture of cytokines , including interleukin 7 ( IL 7 ) , IL 1alpha , IL 6 , granulocyte macrophage colony stimulating factor , and stem cell factor , results in predominant generation of DCs . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CD34+ cells were stimulated with basic fibroblast growth factor ( bFGF ) , stem cell factor / c kit ligand ( SCF ) , interleukin 3 ( IL 3 ) , IL 6 , leukemia inhibitory factor ( LIF ) , and granulocyte monocyte colony stimulating factor ( GM CSF ) alone or in combination in short term serum free liquid suspension cultures ( LSC ) immediately after irradiation and then assayed for clonogenic progenitors . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
A combination of stem cell factor and granulocyte colony stimulating factor enhances the growth of human progenitor B cells supported by murine stromal cell line MS 5 . ^^^ Intriguingly , the addition of recombinant human stem cell factor ( rhSCF ) and granulocyte colony stimulating factor ( rhG CSF ) , but not rhIL 7 , strikingly enhanced the growth of progenitor B cells from CB CD34+ population cultured on MS 5 stromal cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Bone marrow mononuclear cells were cultured with combination of human recombinant ( rhu ) erythropoietin ( EPO ) , granulocyte monocyte colony stimulating factor ( GM CSF ) , granulocyte colony stimulating factor ( G CSF ) , Interleukin 3 ( IL 3 ) , either with or without stem cell factor ( SCF ) , and burst forming unit of erythroid ( BFU E ) growth was assessed . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We used the competitive repopulation assay in mice to test the ability of granulocyte macrophage colony stimulating factor ( GM CSF ) and stem cell factor ( SCF ) , cytokines given in clinical settings to enhance marrow recovery after XRT or BMT and to protect the marrow repopulating ability of primitive hematopoietic stem cells ( PHSC ) after these modalities . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Mice were treated with every combination of recombinant human ( rh ) erythropoietin ( EPO ) , rh granulocyte colony stimulating factor ( G CSF ) , recombinant rat ( rr ) stem cell factor ( SCF ) , and rh interleukin ( IL ) 11 by continuous infusion over 7 days ( full factorial design with three dose levels for each cytokine ) . ^^^ Interactions of erythropoietin , granulocyte colony stimulating factor , stem cell factor , and interleukin 11 on murine hematopoiesis during simultaneous administration . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The addition of stem cell factor ( SCF ) and granulocyte colony stimulating factor ( G CSF ) highly enhanced the production of CD19+ cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We previously reported the aberrant growth of granulocyte macrophage ( GM ) progenitors induced by a combination of stem cell factor ( SCF ) and granulocyte macrophage colony stimulating factor ( GM CSF ) in juvenile chronic myelogenous leukemia ( JCML ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The growth response of colony forming cells to varying concentrations of defined growth factors ( interleukin 3 , interleukin 6 , granulocyte macrophage colony stimulating factor , stem cell factor , and erythropoietin ) was determined , as well as the frequency of burst forming units erythroid , colony forming units granulocyte macrophage , and total colony forming cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We have compared the effects of ATRA and GCSF on the colony growth of 10 MDS marrows , in semi solid and liquid serum free mononuclear cell ( MNC ) cultures , supplemented with a mixture of stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) and granulocyte monocyte colony stimulating factor ( GmCSF ) ( SIGm mix ) , which is fully supportive for myeloid and erythroid ( with erythropoietin ( EPO ) ) colony formation in normal marrow . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Hematopoietic growth factors , such as granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) or stem cell factor ( SCF ) , have been revealed to stimulate the growth of leukemic blast progenitors . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
BACKGROUND : To explore the effect of cytokine therapy on the NADPH oxidase in mature myeloid cells , we isolated neutrophils from patients receiving recombinant human granulocyte colony stimulating factor ( G CSF ) and recombinant human stem cell factor ( SCF ) and evaluated oxidase activity . ^^^ The effects of stem cell factor and granulocyte colony stimulating factor therapy on the activity of the neutrophil NADPH oxidase enzyme system . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We have recently established the culture system to generate dendritic cells ( DCs ) from murine Lin c kit+ bone marrow hematopoietic progenitor cells ( HPCs ) in the presence of granulocyte macrophage colony stimulating factor ( GM CSF ) + stem cell factor ( SCF ) + tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Granulocyte macrophage colony stimulating factor or stem cell factor ( SCF ) alone could stimulate proliferation of HML / SE cells , however interleukin 3 , interleukin 6 , granulocyte colony stimulating factor and thrombopoietin could not . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
PU . 1 / myeloid progenitors can proliferate in vitro in response to the multilineage cytokines interleukin 3 ( IL 3 ) , IL 6 and stem cell factor but are unresponsive to the myeloid specific cytokines granulocyte macrophage colony stimulating factor ( GM CSF ) , G CSF and M CSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
To address the possibility that SCL may function upstream of c kit , we sought to modulate endogenous SCL function in the CD 34 ( + ) hemopoietic cell line TF 1 , which requires SF , granulocyte / macrophage colony stimulating factor , or interleukin 3 for survival . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In order to analyze these effects we studied the levels of interleukin 3 ( IL 3 ) , interleukin 6 , granulocyte macrophage colony stimulating factor , erythropoietin , stem cell factor , soluble vascular adhesion molecule 1 , soluble intercellular adhesion molecule 1 , soluble E selectin and soluble P selectin in 7 SS patients before and during 5 months of HU treatment . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Hematopoietic cytokines ( stem cell factor , interleukin 3 , granulocyte macrophage colony stimulating factor [ GM CSF ] , and granulocyte colony stimulating factor [ G CSF ] contribute to upregulation of CD 95 on bone marrow cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We confirmed functionally that HGF / SF alone has no effect on proliferation of HPCs , but that when combined with granulocyte / macrophage colony stimulating factor ( GM CSF ) or interleukin 3 it acts as a synergistic proliferative factor , although not as potently as kit ligand or FLT 3 / FLK 2 ligand . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Blasts were cultured for 2 weeks in a combination of cytokines c Kit ligand , interleukin 3 and granulocyte macrophage colony stimulating factor ( SIGm mix ) together with all trans retinoic acid or 1alpha , 25 dihydroxy vitamin D 3 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In this study , we have investigated cell reactivity and proliferation with G CSF , granulocyte macrophage colony stimulating factor ( GM CMF ) , macrophage colony stimulating factor ( M CSF ) , stem cell factor ( SCF ) and thrombopoietin ( TPO ) in cases of AML . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The CD 14 dependent and independent dendritic cell ( DC ) pathways are instituted simultaneously when CD 34 ( + ) progenitor cells are treated with granulocyte macrophage colony stimulating factor ( GM CSF ) / tumor necrosis factor ( TNF ) + / stem cell factor ( SCF ) ( GTS ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Marrow stromal cells of patients treated by autologous bone marrow transplantation ( ABMT ) for malignancies have been assessed for their ability to secrete granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , stem cell factor ( SCF ) , leukemia inhibitory factor ( LIF ) , interleukin 6 ( IL 6 ) , transforming growth factor beta 1 ( TGFbeta 1 ) and macrophage inflammatory protein 1alpha . ( MIP 1alpha ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Cord blood CD34+ cells were cultured with granulocyte macrophage colony stimulating factor ( GM CSF ) and tumor necrosis factor alpha ( TNF alpha ) , to which stem cell factor and Flt 3 ligand were added for 5 days . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In this study , normal dogs were given retrovirally transduced bone marrow cells with and without preharvest mobilization by the canine growth factors granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
A number of haematopoietic factors are described that increase the burst number : interleukin 3 ( IL 3 ) , stem cell factor ( SCF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , IL 9 , IL 11 , insulin like growth factor 1 , and erythroid potentiating activity ( EPA ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) / granulocyte colony stimulating factor ( G CSF ) mobilized and CD 34 enriched PB cells were divided into two equal aliquots and transduced with one of two retroviral vectors carrying the neomycin resistance gene ( neo ) for 4 days in the presence of interleukin 3 ( IL 3 ) , IL 6 , and SCF in the first 5 animals , IL 3 / IL 6 / SCF / Flt 3 ligand ( FLT ) in 2 subsequent animals , or IL 3 / IL 6 / SCF / FLT plus an autologous stromal monolayer ( STR ) in the final 2 . ^^^ Stem cell factor ( SCF ) / granulocyte colony stimulating factor ( G CSF ) mobilized and CD 34 enriched PB cells were divided into two equal aliquots and transduced with one of two retroviral vectors carrying the neomycin resistance gene ( neo ) for 4 days in the presence of interleukin 3 ( IL 3 ) , IL 6 , and SCF in the first 5 animals , IL 3 / IL 6 / SCF / Flt 3 ligand ( FLT ) in 2 subsequent animals , or IL 3 / IL 6 / SCF / FLT plus an autologous stromal monolayer ( STR ) in the final 2 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Target cells in this assay were CML cells that were cultured in the presence of stem cell factor , interleukin 3 , granulocyte macrophage colony stimulating factor , granulocyte colony stimulating factor , and erythropoietin . [ 3H ] thymidine incorporation at day 7 represented the proliferation of the progeny of the CD 34 ( + ) CML progenitor cells , and not of the more mature CD 34 ( ) CML cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In addition we examined the in vitro effect of MGDF as well as the combined effect of MGDF and granulocyte colony stimulating factor ( G CSF ) or stem cell factor ( SCF ) on leukemic blast proliferation , recruitment into S phase , induction of programmed cell death and activation of signal transducers and activators of transcription ( STAT ) proteins . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
These cells were cultured with either the HS 5 stromal cell line serum deprived conditioned media supplemented with 10 ng / mL kit ligand ( HS 5CM / KL ) or a recombinant growth factor mix ( GFmix ) containing 10 ng / mL each of interleukin ( IL ) 1 , IL 3 , IL 6 , KL , granulocyte colony stimulating factor , granulocyte macrophage colony stimulating factor , and 3 U / mL of erythropoietin . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
When proliferation was enhanced by growth promoting cytokines ( e . g . granulocyte macrophage colony stimulating factor , GM CSF , stem cell factor , SCF , or PIXY 321 ) , some previously TGF beta 1 sensitive cell lines acquired cellular resistance toward TGF beta 1 mediated growth suppression , whereas four other cell lines remained susceptible to TGF beta 1 growth inhibition despite possible counteraction by other cytokines . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We used a porcine microvascular endothelial cell ( PMVEC ) line in conjunction with exogenous interleukin ( IL ) 3 , IL 6 , granulocyte macrophage colony stimulating factor ( GM CSF ) , and stem cell factor ( SCF ) to expand human HSCs that express the CD 34 and Thy 1 antigens but lack lineage associated markers ( CD34+Thy 1+Lin cells ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Baboon marrow was harvested after in vivo administration ( priming ) of stem cell factor ( SCF ) and granulocyte colony stimulating factor ( G CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Among a panel of growth regulatory factors tested ( granulocyte macrophage colony stimulating factor [ GM CSF ] , IL 3 , granulocyte colony stimulating factor , stem cell factor , and insulin like growth factor 1 ) GM CSF was the only molecule for which both an inhibition of spontaneous BFU E formation by its respective antibody as well as a significant restimulation of erythroid colonies in IL 10 treated cultures by exogenous addition was found . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Because stem cell factor , interleukin 6 ( IL 6 ) , and oncostatin M , but not IL 3 , IL 11 , leukemia inhibitory factor , granulocyte colony stimulating factor , granulocyte macrophage colony stimulating factor , thrombopoietin , and Flk 2 ligand were detected in reverse transcription polymerase chain reaction analysis of AGM S 3 cells , the cells seem to express species cross reactive molecule ( s ) other than the cytokines examined and which act on primitive hematopoietic progenitor / stem cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Circulating CD34+ cell populations characterized by a low rate ( up to five ) or high rate ( more than five ) of cell divisions were isolated from 8 d cultures in the presence of stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , granulocyte colony stimulating factor ( G CSF ) , erythropoietin ( EPO ) , Flt 3 ligand and Peg rHu megakaryocyte growth and development factor ( Peg rHuMGDF ) using the fluorescent dye 5 , 6 carboxyfluorescein diacetate succinimidyl ester ( CFDA SE ) and flow cytometric cell sorting . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
OBJECTIVE : We have investigated the effects of interleukin ( IL ) 10 , IL 4 + granulocyte / macrophage colony stimulating factor ( GM CSF ) and tumour necrosis factor alpha ( TNF alpha ) on the phenotype and antigen presenting capacity of synovial fluid ( SF ) macrophages from patients with rheumatoid arthritis . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Using the adhesive interaction of a cytokine dependent human hemopoietic cell line to immobilized fibronectin , we have analyzed the requirements in divalent cations Mn2+ , Mg2+ and Ca2+ for alpha 4 beta 1 and alpha 5 beta 1 activation by `` inside out ' ' mechanisms triggered by cytokines such as granulocyte macrophage colony stimulating factor or KIT ligand , or by external conformational constraints with the function activating anti beta 1 integrin monoclonal antibody 8A2 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Replating potential of colony forming units of granulocytes / macrophages ( CFU GM ) expanded ex vivo by stem cell factor , interleukin ( IL ) 3 , IL 6 , granulocyte colony stimulating factor , erythropoietin with or without thrombopoietin . ^^^ CD34+ cells isolated from cord blood were cultured in Iscove ' s modified Dulbecco ' s medium supplemented with 10 % fetal bovine serum , 1 . 0 % bovine serum albumin , 50 ng / ml stem cell factor , 50 ng / ml interleukin 3 ( IL 3 ) , 50 ng / ml IL 6 , 100 ng / ml granulocyte colony stimulating factor , and 3 U / ml erythropoietin for 0 , 5 , 7 , 10 , 14 , and 21 days . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We here report that the in vitro proliferative potential of r metHuSCF ( stem cell factor ) plus filgrastim ( granulocyte colony stimulating factor ; r metHuG CSF ) mobilized peripheral blood ( PB ) CD 34 ( + ) cells obtained from previously heavily treated non Hodgkin ' s lymphoma patients inversely correlates with extent of prior therapy . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
By reverse transcription polymerase chain reaction , the expression by MMHs of cytokines involved in the survival and self renewal of early progenitor cells ( stem cell factor and FLT 3 ligand ) as well as those acting at different stages of progenitor differentiation ( interleukin [ IL ] 1beta , IL 3 , leukemia inhibitory factor , IL 6 , granulocyte macrophage colony stimulating factor , granulocyte colony stimulating factor , macrophage colony stimulating factor , and thrombopoietin ) was shown . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
When combinations of cytokines were used over 7 days , between 5 % ( FLT 3 ligand [ FLT 3L ] , stem cell factor [ SCF ] , IL 3 , IL 6 , granulocyte colony stimulating factor [ G CSF ] , beta nerve growth factor [ betaNGF ] ) and 22 % ( FLT 3L + HGF ) of the cells remained undivided , between 15 % ( HGF , IL 1 , IL 11 , G CSF ) and 68 % ( SCF + TPO ) of the cells had doubled and between 27 % ( FLT 3L + TPO ) and 70 % ( HGF , IL 1 , IL 11 , G CSF ) died . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Since cytokines are of crucial significance in this process , we decided to examine the role of stem cell factor ( SCF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) , granulocyte colony stimulating factor ( G CSF ) , and interleukin 1 alpha ( IL 1 alpha ) , known for their stimulatory effects on in vitro hematopoiesis ; and transforming growth factor beta ( TGF beta ) , interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) , known for their inhibitory effect , in the anemia of GCA . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Cultures of sorted cells , supplemented with granulocyte colony stimulating factor ( G CSF ) / granulocyte macrophage colony stimulating factor ( GM CSF ) / IL 1 / IL 3 / IL 6 / stem cell factor ( SCF ) and erythropoietin ( EPO ) indicated that the LFA 1 ( neg ) fraction contained the majority of the colony forming cells ( CFCs ) ( LFA 1 ( neg ) 183 + / 62 / 7 , 500 cells 5 LFA 1 ( pos ) 29 + / 17 / 7 , 500 cells , P < . 001 ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Additional neutralizing antibody studies suggest that MDA is not one of the cytokines known to induce some degree of megakaryopoiesis in vitro or in vivo including interleukin 3 ( IL 3 ) , IL 6 , IL 11 , granulocyte macrophage colony stimulating factor , erythropoietin , or stem cell factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The interaction between SCF and TPO on the neutrophilic cell generation was greater than the combined effects of SCF with granulocyte colony stimulating factor ( G CSF ) or granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
DCs were generated ex vivo in the presence of tumor necrosis factor alpha , granulocyte macrophage colony stimulating factor , stem cell factor , and flk 2 / flt 3 ligand taken from CD34+ hematopoietic progenitors that were mobilized into the peripheral blood of cancer patients treated with high dose cyclophosphamide and filgrastim . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Cryopreserved CB CD 34 ( + ) cells as well as freshly isolated CB CD 34 ( + ) cells cultured with granulocyte macrophage colony stimulating factor ( GM CSF ) / stem cell factor ( SCF ) / tumor necrosis factor alpha ( TNF alpha ) for 14 days gave rise to CD1a+ / CD4 ( + ) / CD11c+ / CD14 ( ) / CD40 ( + ) / CD80 ( + ) / CD83 ( + ) / CD86 ( + ) / HLA DR+ cells with dendritic morphology . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Two animals in each group were treated as follows : 200 or 500 microg / kg FL , 10 microg / kg granulocyte colony stimulating factor ( G CSF ) , 10 or 75 microg / kg stem cell factor ( SCF ) , 10 microg / kg G CSF + 500 microg / kg FL , 10 microg / kg G CSF + 75 microg / kg SCF + 500 microg / kg FL . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We have recently demonstrated that CD11b / dullCD11c+ and CD11b+hiCD11c+ dendritic cell ( DC ) precursor subsets represent two distinct DC differentiation pathways from murine bone marrow lineage phenotype negative ( Lin ) c kit+ hematopoietic progenitor cells ( HPCs ) stimulated with granulocyte macrophage colony stimulating factor ( GM CSF ) + stem cell factor ( SCF ) + tumor necrosis factor alpha ( TNFalpha ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Although granulocyte colony stimulating factor ( G CSF ) , interleukin ( IL ) 6 , IL 7 , and IL 11 synergize with SCF to increase blast colony formation , cytokines such as granulocyte macrophage CSF or IL 3 are without significant effect . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The results show that using a growth factor combination including granulocyte macrophage colony stimulating factor plus tumor necrosis factor alpha plus interleukin 4 plus stem cell factor plus Flt 3 ligand , more than 70 % of DCs derived from CB or MPB CD34+ cells can be transduced . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Culturing cord blood CD34+ cells with granulocyte macrophage colony stimulating factor ( GM CSF ) and tumor necrosis factor ( TNF ) alpha for 12 days , and stem cell factor ( SCF ) for 5 days , resulted in a 40 + / 26 fold expansion in cell numbers , with 38 + / 20 % dendritic cells ( DCs ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
To examine whether progenitor homing was altered upon ex vivo expansion , highly enriched Sca 1 ( + ) c kit+Lin cells were cultured for 9 days in serum free medium containing interleukin ( IL ) 6 , IL 11 , granulocyte colony stimulating factor , stem cell factor , flk 2 / flt3 ligand , and thrombopoietin . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In vitro growth of myeloid leukemic cell lines and of blasts from 14 primary AMLs demonstrated that recombinant human leptin alone induced low level proliferation , significantly ( P < . 05 ) increased proliferation induced by recombinant human granulocyte colony stimulating factor , interleukin 3 , and stem cell factor in a subset of AML and increased colony formation ( P < . 005 ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Synergistic stimulatory effects of TPO with stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , granulocyte colony stimulating factor and granulocyte macrophage colony stimulating factor were observed in both TPO responding cases ( 9 / 17 ) and TPO non responding cases ( 8 / 13 ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Human cord blood CD 34 ( + ) stem cells were allowed to differentiate in the presence of cytokines stem cell factor ( SCF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) and tumour necrosis factor alpha ( TNF alpha ) into functional CD1a+dendritic cells ( DC ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Different culture conditions were generated by adding G CSF , granulocyte macrophage colony stimulating factor ( GM CSF ) , Flt 3 ligand ( Flt 3 ) , megakaryocyte growth and development factor ( Peg rHuMGDF ) , GM CSF / erythropoietin ( EPO ) hybrid protein ( MEN 11303 ) , and interleukin 15 ( IL 15 ) to the basic stem cell factor ( SCF ) + interleukin 3 ( IL 3 ) + EPO combination . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Marked up regulation of growth factors , cytokines and receptor expression was observed in thick ( > 1 mm ) primary melanomas , which were stained with polyclonal or monoclonal antibodies ( MAbs ) for IL 1alpha , IL 1beta , IL 6 , IL 8 , TNF alpha , TGF beta , granulocyte macrophage colony stimulating factor ( GMCSF ) and stem cell factor ( SCF ) , but not IL 2 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Other cytokines stimulating megakaryocytopoiesis in various species using different dosing regimens include Interleukin 1 ( IL 1 ) , Interleukin 3 ( IL 3 ) , Interleukin 6 ( IL 6 ) , Interleukin 11 ( IL 11 ) , stem cell factor ( SCF ) , granulocytemacrophage colony stimulating factor , ( GM CSF ) , fusion proteins , such as promegapoietin , and a peptide agonist of TPO receptor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Prolonged release and c kit expression of haemopoietic precursor cells mobilized by stem cell factor and granulocyte colony stimulating factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We found that granulocyte macrophage colony stimulating factor ( GM CSF ) optimally activated proliferation of TF 1 cells regardless of the presence or absence of stem cell factor ( SCF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
First , we investigated whether CD 34 ( + ) stem / progenitor cells express gelatinases A ( MMP 2 ) and / or B ( MMP 9 ) and whether growth factors and cytokines ( granulocyte colony stimulating factor [ G CSF ] , granulocyte macrophage colony stimulating factor [ GM CSF ] , stem cell factor [ SCF ] , macrophage colony stimulating factor [ M CSF ] , interleukin 3 [ IL 3 ] , IL 6 , IL 8 , and tumor necrosis factor alpha [ TNF alpha ] ) are able to modulate their expression . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The recombinant ovine ( rov ) SCF protein has been expressed in Chinese hamster ovary ( CHO ) cells , purified , and its biological activity on ovine bone marrow cells compared with that of interleukin 3 ( rovIL 3 ) , granulocyte macrophage colony stimulating factor ( rovGM CSF ) , interleukin 5 ( rovIL 5 ) , human macrophage colony stimulating factor ( M CSF ) and human erythropoietin ( epo ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
After 6 7 days of culturing the CD34+ cells of HNSCC patients with granulocyte macrophage colony stimulating factor , stem cell factor , and tumor necrosis factor at , there appeared CD14+CD1a+ and a lesser proportion of CD 14 ( ) CD1a+ cells resembling the precursor cells of the bipotential and committed dendritic cell differentiation pathways that have been described for cord blood CD34+ cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In this trial , stem cell mobilization with granulocyte colony stimulating factor ( G CSF ) alone was compared to mobilization with G CSF combined with stem cell factor ( SCF ) in heavily pretreated patients with Hodgkin ' s and non Hodgkin ' s lymphoma . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Impaired response of granulocyte committed progenitor cells to stem cell factor and granulocyte colony stimulating factor in human cyclic neutropenia . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We have assessed tumor contamination of peripheral blood progenitor cells ( PBPC ) in 203 high risk breast cancer patients who were prospectively randomized to mobilization with stem cell factor ( SCF ) plus granulocyte colony stimulating factor ( G CSF ) versus G CSF alone . ^^^ Incidence of tumor cell contamination in leukapheresis products of breast cancer patients mobilized with stem cell factor and granulocyte colony stimulating factor ( G CSF ) or with G CSF alone . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The present study focused on whether it is possible to expand monocytic cells from CD34+ progenitor cells by using macrophage colony stimulating factor ( M CSF ) in the absence and presence of mast cell growth factor ( MGF ) and IL 6 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Stimulation of human hematopoietic growth factor responsive cell lines with stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , and granulocyte macrophage colony stimulating factor ( GM CSF ) demonstrate the rapid tyrosine phosphorylation of SHIP 2 and its resulting association with SHC . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The cytokines macrophage colony stimulating factor ( M CSF ) and stem cell factor ( SCF ) bind to related members of this tyrosine kinase receptors family , c fms and c kit , respectively . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
When fresh or thawed cells are cultured with granulocyte macrophage colony stimulating factor and stem cell factor with or without tumour necrosis factor alpha ( TNF alpha ) , they generate numerous cells with the characteristic ultrastructural , phenotypic and functional properties of dendritic cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
First , Baf cells only proliferated in response to BAC OV IL 5 and did not respond to addition of recombinant ovine cytokines granulocyte macrophage colony stimulating factor ( GM CSF ) , IL 1beta , IL 2 , IL 3 , IL 6 , IL 8 , stem cell factor ( SCF ) or IFN gamma at doses from 0 . 01 to 1 microg / well . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In a second step , we established three different cytokine combinations by adding the megakaryocyte growth and development factor + / erythropoietin and granulocyte colony stimulating factor to a fetal calf serum containing medium with Flt 3 , stem cell factor , and interleukin 3 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Monocytic differentiation of CD 34 ( + ) cells supported by FLT 3 ligand and interleukin 3 ( IL 3 ) was correlated to high levels of hypophosphorylated pRb , whereas neutrophilic differentiation , supported by granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) , was correlated to low levels . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Normal bone marrow cells or Thy 1 . 1lowSca 1+Lin ( neg ) HSPC were incubated in methylcellulose cultures and stimulated with various combinations of steel factor ( SF ) , interleukin ( IL ) 3 , IL 6 , granulocyte colony stimulating factor ( G CSF ) , and erythropoietin ( EPO ) in the presence of increasing concentrations of Cy A . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Recombinant human cytokines stem cell factor ( SCF ) , pegylated megakaryocyte growth and differentiation factor ( PEG MGDF ) , FLT 3 L , Interleukin ( IL ) 3 , IL 6 , and granulocyte macrophage colony stimulating factor ( GM CSF ) were systematically added at various concentrations ( 10 to 300 ng / mL ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Co expression of CD 7 on CD34+ cells was induced to decrease significantly after short term in vitro culture with the differentiation inducing agent phorbol ester ( PMA ) and with a combination of cytokines ( stem cell factor , interleukin 3 and granulocyte colony stimulating factor ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The c mpl ligand , thrombopoietin ( TPO ) , is a physiologic regulator of platelet and megakaryocytic production , acting synergistically on thrombopoiesis with the growth factors interleukin 11 ( IL 11 ) , stem cell factor , interleukin 3 ( IL 3 ) , interleukin 6 ( IL 6 ) , and granulocyte macrophage colony stimulating factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In short term cultures containing erythropoietin ( Epo ) , interleukin ( IL ) 1 , IL 3 , and IL 6 , or granulocyte ( G ) and granulocyte macrophage colony stimulating factor ( GM CSF ) together with stem cell factor ( SCF ) or Flt 3 ligand ( FL ) , expansion of CFUs was six to eightfold at week 1 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The Th 2 cytokines IL 3 , IL 5 , IL 6 , IL 9 , and granulocyte / macrophage colony stimulating factor each augmented the SCF dependent proliferation of hPrMCs and hMCs . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CIS 1 is induced by interleukin 2 ( IL 2 ) , IL 3 , GM CSF , and erythropoietin ( EPO ) , but not by IL 6 , granulocyte colony stimulating factor ( G CSF ) , or stem cell factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In the presence of submaximal concentrations of recombinant human granulocyte macrophage colony stimulating factor ( rhGM CSF ; 0 . 5 ng / ml ) , PGG glucan significantly increased BMMC myeloid colony formation comparable to the increase observed with either interleukin 3 ( rhIL 3 ) or stem cell factor ( rhSCF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In neutralizing experiments on HUVEC derived cytokines , antibodies to both stem cell factor ( SCF ) and granulocyte / macrophage colony stimulating factor ( GM CSF ) were added to the cell cultures . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Growth factors such as stem cell factor ( SCF ) , granulocyte macrophage colony stimulating factor , and thrombopoietin were reported to upregulate the adhesion of hematopoietic progenitors to immobilized fibronectin through activation of integrin alpha4beta1 and alpha5beta1 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
However , the cellular growth rate was significantly accelerated in the presence of various growth factors such as granulocyte colony stimulating factor , stem cell factor , granulocyte macrophage colony stimulating factor , interleukin 3 , and thrombopoietin . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Selected progenitors were then cultured in the mixed colony assay with incremental concentrations of combinations containing erythropoietin ( Epo ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) and c kit ligand . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In 2 animals , cells of the different fractions were transduced in the presence of human FLT 3 ligand ( FLT3L ) , canine stem cell factor ( cSCF ) , and human megakaryocyte growth and development factor ( MGDF ) , and in 2 other dogs , transduction was performed in the presence of FLT3L , cSCF , and canine granulocyte colony stimulating factor ( cG CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
SVZ neural precursor cells could not be generated with platelet derived growth factor ( PDGF ) , granulocyte macrophage colony stimulating factor , stem cell factor , heparin binding epidermal growth factor ( HB EGF ) , granulocyte colony stimulating factor , or ciliary neurotrophic factor ( CNTF ) , but could be with EGF , fibroblast growth factor 2 ( FGF 2 ) , and FGF 2 plus heparin . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
OBJECTIVES : The purpose of this study was to analyse the presence of the granulocyte macrophage colony stimulating factor ( GM CSF ) in human cerebrospinal fluid ( SF ) of patients affected by multiple sclerosis ( MS ) in comparison with non inflammatory neurological diseases . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
After single cell sorting , the CD 34 ( + ) / CD38 ( ) cells derived from FLV were exposed to flt 3 ligand , thrombopoietin , stem cell factor ( SCF ) , or medium containing a cytokine cocktail ( with SCF , interleukin 3 , interleukin 6 , granulocyte macrophage colony stimulating factor , and erythropoietin ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
METHODS : To obtain large numbers of pig hematopoietic cells , leukapheresis was used to collect blood cell products in miniature swine ( n = 5 ) after progenitor cell mobilization by use of a course of hematopoietic growth factors ( cytokines ) , consisting of porcine interleukin 3 , porcine stem cell factor , and human granulocyte colony stimulating factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Constitutive Cdc25A protein levels were enhanced with granulocyte macrophage colony stimulating factor ( GM CSF ) plus steel factor ( SF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We have generated DC from human umbilical cord blood CD34+ progenitors cultured with granulocyte macrophage colony stimulating factor , tumor necrosis factor alpha ( TNF alpha ) , and stem cell factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Colonies grown from progenitor cells cultured in methylcellulose were determined for 14 days in the presence or absence of growth factors , including granulocyte monocyte colony stimulating factor , stem cell factor , and interleukin 3 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
A murine bone marrow stromal cell line , HESS 5 , supports the survival and proliferation of Lin ( ) CD 34 ( ) cells in the presence of fetal calf serum and human cytokines thrombopoietin , Flk 2 / Flt 3 ligand , stem cell factor , granulocyte colony stimulating factor , interleukin 3 , and interleukin 6 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We describe here that lineage phenotype negative ( Lin ) ( ) c kit ( + ) hematopoietic progenitor cells ( HPCs ) from day 13 postcoitus ( dpc ) murine fetal liver ( FL ) can generate dendritic cell ( DC ) precursors when cultured in vitro in the presence of PA 6 stromal cells plus granulocyte / macrophage colony stimulating factor ( GM CSF ) + stem cell factor ( SCF ) + Flt 3 ligand ( Flt3L ) for 12 to 14 days , and develop into mature DCs when stimulated with GM CSF plus mouse tumor necrosis factor alpha ( mTNFalpha ) for an additional 3 to 5 days . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Medium for the ex vivo cultures contained either serum and six recombinant human hematopoietic growth factors ( GFs ) , including Flt 3 ligand ( FL ) , Kit ligand ( KL = stem cell factor ) , thrombopoietin ( Tpo ) , interleukin 3 ( IL 3 ) , granulocyte colony stimulating factor ( G CSF ) , and interleukin 6 ( IL 6 ) , or a serum free medium containing only FL , KL , and Tpo . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In vitro proliferation and differentiation of cord blood derived CD 34 ( + ) cells ( induced by stem cell factor and granulocyte macrophage colony stimulating factor ) were consistently inhibited by the addition of anti p75 / AIRM1 mAb . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Culture of bone marrow precursor cells with cytokines , including granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 4 ( IL 4 ) and the tyrosine kinase receptor binding proteins Flt 3 ligand ( Flt 3L ) and stem cell factor ( SCF ) , has previously been shown , in both mouse and human , to result in the generation of large numbers of dendritic cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Significant growth responses of leukemic blasts to TPO were seen in 10 / 10 CD7+ and 7 / 20 CD 7 AML cases using 3H thymidine incorporation , while synergistic stimulatory effects of TPO with stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , granulocyte colony stimulating factor , and granulocyte macrophage colony stimulating factor were observed in both groups . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
When cultured with the single cytokines as stem cell factor ( SCF ) , TPO , or interleukin ( IL ) 3 , these cells were maintained or had increased by day 5 , whereas only a few cells survived in cultures with granulocyte colony stimulating factor , IL 11 , or IL 6 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We found that the cytokines stem cell factor ( maximal response with 1 mM ) , granulocyte colony stimulating factor ( 0 , 1 mM ) and erythropoietin ( 1 mM ) relaxed , while tumor necrosis factor alpha ( 0 . 1 mM ) , interleukin ( IL ) 6 ( 10 mM ) and IL 10 ( 0 . 1 mM ) induced contraction of arterial but not of venous segments . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We found no neutralization of granulocyte colony stimulating factor , macrophage colony stimulating factor , stem cell factor , IL 1beta , IL 2 , IL 3 , IL 4 , IL 6 , IL 9 , IL 10 , IL 12 , tumour necrosis factor alpha , oncostatin M ( OSM ) and IFN gamma . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CD34+ cells from a total of 93 patients with either acute myeloid leukemia ( AML ; n = 25 ) , chronic myeloid leukemia ( CML ; n = 21 ) , chronic lymphocytic leukemia ( CLL ; n = 18 ) , polycythemia vera ( PV ; n = 16 ) , or myelodysplastic syndromes ( MDS ; n = 13 ) were analyzed before and in 19 patients after ex vivo expansion in the presence of multiple cytokines ( kit ligand , interleukin 3 , interleukin 6 , and granulocyte colony stimulating factor plus erythropoietin ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The combination of cytokines flt 3 ligand + c kit ligand + granulocyte macrophage colony stimulating factor ( GM CSF ) + interleukin 4 ( IL 4 ) + tumor necrosis factor alpha ( TNF alpha ) was used to maximize the output of mature DCs in the culture of CD34+ cells while minimizing the production of monocytes . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Murine bone marrow cells were incubated at 1 % oxygen in liquid medium supplemented with stem cell factor , granulocyte colony stimulating factor , interleukin 6 and interleukin 3 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Interleukin 1 , interleukin 6 , granulocyte monocyte colony stimulating factor , stem cell factor , scatter factor / hepatocyte growth factor and transforming growth factor beta , added separately to MCF 7 / AZ cells , could not mimic the effects of CM COLO 16 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : The effects of pH AND pO 2 on granulocyte proliferation , differentiation , and granulocyte colony stimulating factor receptor ( G CSFR ) expression were evaluated using mobilized peripheral blood CD 34 ( + ) cells directed down the granulocytic pathway with stem cell factor , interleukin 3 , interleukin 6 , and G CSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Incubation ( 24 h ) with interleukin 3 ( IL 3 ) , recombinant human stem cell factor ( SCF ) and granulocyte colony stimulating factor ( G CSF ) significantly increased the proportion of cells in the S phase for both CFC and LTC IC without inducing any loss in numbers . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We have shown that the sequential use of early acting hematopoietic growth factors , stem cell factor , IL 3 and IL 6 , followed by differentiation with IL 4 and granulocyte macrophage colony stimulating factor allows the in vitro generation of large numbers of immature DCs from CD 34 ( + ) peripheral blood progenitor cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Single human cord blood CD34+CD38 cells were individually cultured by micromanipulation with interleukin ( IL ) 3 , stem cell factor ( SCF ) , fIt 3 ligand ( FL ) , IL 6 and granulocyte colony stimulating factor ( G CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We preinjected the mice with murine stem cell factor ( SCF ) encoding adenovirus ( AdSCF ) and murine granulocyte macrophage colony stimulating factor ( GM CSF ) encoding adenovirus ( AdGM CSF ) ; after 7 days , the mice were inoculated with CT 26 colon adenocarcinoma . ^^^ Enhanced antitumoral effect of adenovirus mediated cytosine deaminase gene therapy by induction of antigen presenting cells through stem cell factor / granulocyte macrophage colony stimulating factor gene transfer . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
To determine whether cytokine induced proliferation also limits the rate of short term engraftment and potential clinical utility of ex vivo expanded hematopoietic cells , murine Sca 1 ( + ) c kit ( + ) Lin ( ) cells were cultured in interleukin 6 ( IL 6 ) , IL 11 , granulocyte colony stimulating factor ( G CSF ) , stem cell factor , flk 2 ligand , and thrombopoietin for 7 days . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Moreover , addition of any of the hematopoietic cytokines , erythropoietin , stem cell factor , granulocytic colony stimulating factor , or granulomonocytic colony stimulating factor induced a highly significant increase in tube formation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The addition of granulocyte macrophage colony stimulating factor to hyper IL 6 and stem cell factor was essential for the differentiation of expanded progenitor cells into antigen presenting cells capable of inducing a primary T cell response to soluble protein , which is a typical feature of dendritic cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : CD 34 ( + ) PBPC were cultured in serum free medium comparing different ( n = 32 ) combinations of stem cell factor ( S ) , interleukin 1 ( 1 ) , interleukin 3 ( IL 3 ) ( 3 ) , interleukin 6 ( 6 ) , erythropoietin ( E ) , granulocyte colony stimulating factor ( G ) , granulate macrophage colony stimulating factor ( GM ) , daniplestim ( D , a novel IL 3 receptor agonist ) , and Flt 3 ligand ( FL ) under various culture conditions . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Such cytokines included granulocyte macrophage colony stimulating factor , granulocyte colony stimulating factor , interleukin 3 , and stem cell factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We assessed PEG rHuMGDF combined with granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage CSF ( GM CSF ) , interleukin 3 ( IL 3 ) , IL 6 , stem cell factor ( SCF ) or erythropoietin in 40 MDS , 33 AML and 16 normal bone marrow samples . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
BACKGROUND AND OBJECTIVE : Hepatocyte growth factor ( HGF ) is known to augment the effects of stem cell factor , interleukin 3 , granulocyte macrophage colony stimulating factor ( GM CSF ) , erythropoetin , and granulocyte colony stimulating factor , all of which are involved in hematopoiesis . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
HbF was moderately enhanced by interleukin 3 ( IL 3 ) and granulocyte macrophage colony stimulating factor treatment ( up to 5 % 8 % gamma globin content ) , while sharply reactivated in a dose dependent fashion by c kit ligand ( KL ) and NaB ( 20 % 23 % ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The CFU C content could be expanded 50 fold by culture for 1 week in serum deprived medium and the growth factors kit ligand and granulocyte macrophage colony stimulating factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Purified CD34+ haematopoietic progenitor cells were cultivated with stem cell factor , interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) and granulocyte CSF ( G CSF ) for 7 d , and thereafter non adherent cells were divided into two groups . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Their production of M CSF cultured in serum free medium is enhanced in a time dependent manner in response to tumour necrosis factor ( TNF ) alpha and interleukin ( IL ) 4 but not to IL 1 , IL 3 , IL 6 , IL 7 , IL 10 , SCF , granulocyte macrophage colony stimulating factor ( GM CSF ) , G CSF , bFGF and transforming growth factor ( TGF ) beta . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Stem Cell Factor ( SCF ) can stimulate the growth and development of primitive multipotential and unipotential hematopoietic stem cells , either alone or in combination with other cytokines such as Granulocyte Macrophage Colony Stimulating factor ( GM CSF ) and Granulocyte CSF ( G CSF ) . ^^^ Stem Cell Factor ( SCF ) can stimulate the growth and development of primitive multipotential and unipotential hematopoietic stem cells , either alone or in combination with other cytokines such as Granulocyte Macrophage Colony Stimulating factor ( GM CSF ) and Granulocyte CSF ( G CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The adherent stroma cell layer was modified by interleukin 3 ( IL 3 ) + granulocyte macrophage colony stimulating factor ( GM CSF ) + stem cell factor ( SCF ) with an increased cellularity and higher percentage of differentiated myeloid cells and a reduced percentage of stroma cells . ^^^ Effect of interleukin 3 , stem cell factor and granulocyte macrophage colony stimulating factor on committed stem cells , long term culture initiating cells and bone marrow stroma in a one step long term bone marrow culture . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Lin ( ) CD 34 ( ) population isolated from human cord blood was cultured in the presence of murine bone marrow stroma cell line , HESS 5 , and human cytokines , thrombopoietin , Flk2 / Flt3 ligand , stem cell factor , granulocyte colony stimulating factor , interleukin 3 ( IL 3 ) , and IL 6 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Comparisons of structure function relationships of Flt3L with the homologous hematopoietic cytokines macrophage colony stimulating factor ( MCSF ) and stem cell factor ( SCF ) suggest that they have a common receptor binding mode that is distinct from the paradigm derived from the complex of growth hormone with its receptor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Cytokines that appear to have a functional role in mammalian implantation include leukaemia inhibitory factor , interleukin 1 , hepatocyte growth factor , stem cell factor , macrophage colony stimulating factor and insulin like growth factors . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Primitive hematopoietic progenitors were stimulated in liquid cultures and clonogenic assays by early acting growth factors such as stem cell factor ( SCF ) and interleukin 11 ( IL 11 ) and the intermediate late acting stimulating factors IL 3 , granulocyte macrophage colony stimulating factor , and erythropoietin . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We examined the effects of granulocyte colony stimulating factor ( G CSF ) , stem cell factor ( SCF ) , and thrombopoietin ( TPO ) , alone or in combination , on the generation of neutrophils by bone marrow ( BM ) cells from three patients with severe congenital neutropenia ( SCN ) through the use of a serum deprived liquid culture system . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The yield of pre CFUs with the combination of granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) was significantly higher than with G CSF alone , cyclophosphamide ( Cyclo ) and granulocyte monocyte colony stimulating factor ( GM CSF ) , interleukin ( IL ) 3 and GM CSF , or Cyclo alone . ^^^ Combination of stem cell factor and granulocyte colony stimulating factor mobilizes the highest number of primitive haemopoietic progenitors as shown by pre colony forming unit ( pre CFU ) assay . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In this paper the effects of flt 3 ligand ( FL ) , stem cell factor ( SCF ) , interleukin ( IL ) 3 , IL 6 , granulocyte colony stimulating factor ( G CSF ) and granulocyte monocyte colony stimulating factor ( GM CSF ) on long term ex vivo expansion and differentiation of cord blood hematopoietic cells were investigated . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Reassessment of interactions between hematopoietic receptors using common beta chain and interleukin 3 specific receptor beta chain null cells : no evidence of functional interactions with receptors for erythropoietin , granulocyte colony stimulating factor , or stem cell factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Addition of other cytokines , including interleukin 3 ( IL 3 ) , IL 6 , granulocyte macrophage colony stimulating factor , and stem cell factor , to LIF in the cultures caused a 150 fold expansion of cells retaining the CD 34 ( + ) thy 1 ( + ) phenotype . ^^^ Furthermore , we have ruled out the contribution of 6 known early acting cytokines , including interleukin 3 , interleukin 6 , granulocyte macrophage colony stimulating factor , stem cell factor , flt 3 ligand , and thrombopoietin , to this SCEPF activity . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CD34+ bone marrow cells of healthy donors were used to generate DCs with the recombinant human cytokines granulocyte macrophage colony stimulating factor ( GM CSF ) , tumour necrosis factor alpha ( TNF alpha ) and stem cell factor ( SCF ) , both with and without interleukin 4 ( IL 4 ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Compared with hMC maintained in stem cell factor alone , hMC primed with IL 5 expressed 2 to 4 fold higher steady state levels of TNF alpha , IL 5 , IL 13 , macrophage inflammatory protein 1alpha , and granulocyte macrophage colony stimulating factor transcripts 2 h after FcvarepsilonRI crosslinking and secreted 2 to 5 fold greater quantities of the corresponding cytokines , except IL 13 , at 6 h . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The CD 34 ( + ) cells were then transduced in virus containing medium ( gibbon ape leukemia virus pseudotype vector LAPSN [ PG 13 ] ) in wells coated with the recombinant human fibronectin fragment CH 296 and in the presence of multiple hematopoietic growth factors ( interleukin 6 , stem cell factor , granulocyte colony stimulating factor , granulocyte macrophage colony stimulating factor , and megakaryocyte growth and development factor ) and protamine sulfate . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Unselected PBPC were cultured in gas permeable bags containing 1 L serum free media , granulocyte colony stimulating factor , stem cell factor , and pegylated megakaryocyte growth and development factor for 9 days . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The concentrations of stem cell factor , interleukin 3 , interleukin 6 , erythropoietin , and granulocyte colony stimulating factor ( G CSF ) were measured using a commercially available immunoassay . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In cultures of human bone marrow cells stimulated with macrophage colony stimulating factor and stem cell factor , interleukin 6 inhibited colony formation by 51 74 % . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Following high dose chemotherapy administration , we transplanted adult patients with CB expanded in static culture for 10 days , in DM containing stem cell factor ( SCF ) , granulocyte colony stimulating factor ( G CSF ) , and megakaryocyte growth and development factor ( MGDF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
To determine the frequency and responsiveness to cytokines of megakaryocyte progenitors ( CFU Meg ) in TAR syndrome , the authors studied marrow samples from 3 patients and 6 normal controls , using optimally standardized megakaryocyte growth media incorporating interleukin 3 , interleukin 6 , stem cell factor , and granulocyte monocyte colony stimulating factor , with and without pegylated recombinant human megakaryocyte growth and development factor ( PEG rHuMGDF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
MKE did not affect the total number of colonies of erythroid burst forming units ( BFU E ) , granulocyte macrophage colony forming units ( CFU GM ) or granulocyte erythroid macrophage megakaryocyte colony forming units ( CFU Mix / CFU GEMM ) in a methylcellulose culture with exogenous recombinant human granulocyte colony stimulating factor , granulocytemacrophage colony stimulating factor , interleukin 3 , stem cell factor and erythropoietin . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In cultures with SCF , Flt 3 ligand ( FL ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and tumor necrosis factor ( TNF ) alpha ( standard condition ) , CD 7 ( + ) CD45RA ( + ) progenitors expanded less ( 37 vs 155 fold ) but yielded 2 fold higher CD1a ( + ) DC percentages than CD 7 ( ) CD45RA ( + ) or CD 7 ( + ) CD45RA ( ) progenitors . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Nonenriched CB cells were cultured for 21 days in the presence of conditioned medium from the HS 5 stromal cell line and FLT 3 L or alternatively in the presence of FLT 3 L , stem cell factor ( SCF ) , megakaryocite growth and development factor ( MGDF ) , and granulocyte colony stimulating factor ( G CSF ) ( FSMG ) , either on fibronectin fragment CH 296 coated dishes or on uncoated dishes . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In serum free liquid cultures supplemented with stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) and granulocyte colony stimulating factor , the expression of surface CXCR 4 progressively increased in cells differentiating along the granulocytic lineage . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CD34 ( + ) cord blood ( CB ) cells were cultured in serum free medium containing stem cell factor , Flt 3 ligand , megakaryocyte growth and development factor , and granulocyte colony stimulating factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
MSCs constitutively expressed mRNA for interleukin ( IL ) 6 , IL 11 , leukemia inhibitory factor ( LIF ) , macrophage colony stimulating factor ( M CSF ) , and stem cell factor ( SCF ) . ^^^ MSCs treated with IL 1alpha upregulated mRNA levels of IL 6 , IL 11 , and LIF , and began to express detectable levels of granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) . mRNA levels of M CSF and SCF did not change . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In this paper we show the megakaryocytopoietic productivity of small scale in vitro serum free cultures of human CD34+ cells containing MK growth and development factor ( MGDF ) and stem cell factor ( Kit ligand ; SCF ) + / granulocyte colony stimulating factor ( G CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Culture of AML cells in serum free medium with 100 ng / ml Steel factor ( SF ) , 20 ng / ml interleukin 3 ( IL 3 ) and 20 ng / ml granulocyte colony stimulating factor ( G CSF ) for 16 24 h maintained the number of AML CFC and LTC IC at near input values ( mean % input + / s . d . for CFC and LTC IC were 78 + / 33 and 126 + / 53 , respectively ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Therefore BM cells were ( a ) either frozen in liquid nitrogen or ( b ) precultured for 14 days with cytokines : IL 1beta , IL 3 , IL 6 , erythropoietin ( EPO ) , stem cell factor ( SCF ) with ( ' Cytok1 ' ) or without granulocyte macrophage colony stimulating factor ( GM CSF ) ( ' Cytok2 ' ) or with no added cytokines ( ' ISC / FCS ' ) as a control . ( 3 ) Southern blot analysis was used to detect clonal BM cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In vivo priming of target cells , i . e . mobilization of HSC into the circulation with granulocyte colony stimulating factor and stem cell factor , as well as optimization of the in vitro transduction conditions , now allow a stable in vivo gene transfer efficiency of up to 10 15 % in both lymphoid and myeloid circulating cells in non human primates , levels that would be adequate for many clinical applications . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In a second set of experiments , CB derived progenitor cells were incubated with interleukin ( IL ) 3 , stem cell factor ( SCF ) and granulocyte colony stimulating factor ( G CSF ) and their cell cycle status assessed both by the cytosine arabinoside ( Ara C ) suicide approach and by flow cytometric DNA analysis . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The cells were cultured with four cytokines : interleukin 3 ( IL 3 ) , thrombopoietin ( TPO ) , stem cell factor ( SCF ) , and Flt 3 ) ; five cytokines , IL 3 , TPO , SCF , Flt 3 plus granulocyte macrophage colony stimulating factor ( GM CSF ) , or erythropoietin ( Epo ) ; or all six cytokines in combination . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Candidate stem cells were transduced immediately after isolation or after up to 4 days of culture in serum deprived medium containing the growth factors kit ligand and granulocyte macrophage colony stimulating factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We discuss recent data indicating that engagement of p75 / AIRM1 or CD 33 sharply inhibits the in vitro proliferation / differentiation of CD34+ myeloid precursors induced by stem cell factor and granulocyte macrophage colony stimulating factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Results of this study indicate that expression of vascular cell adhesion molecule 1 ( VCAM 1 ) is strongly reduced in vivo in the BM during HPC mobilization by granulocyte colony stimulating factor ( G CSF ) and stem cell factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CD 34 ( + ) cells from the bone marrow of 22 RA patients differentiated into cells with fibroblast like morphology , which expressed prolyl 4 hydroxylase , in the presence of stem cell factor ( SCF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and tumor necrosis factor alpha ( TNF alpha ) , much more effectively than CD 34 ( + ) cells from bone marrow of 15 control subjects ( 10 patients with osteoarthritis and 5 healthy individuals ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In the present study , we examined the effect of IL 3 on human B lymphopoiesis using the co culture system on a murine stromal cell line , MS 5 , with stem cell factor ( SCF ) and granulocyte colony stimulating factor ( G CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The present study was undertaken to : ( 1 ) compare the effect of some hematopoietic growth factors , like interleukine 3 , thrombopoietin , granulocyte megakaryocyte colony stimulating factor , stem cell factor , and reactive oxygen species such as H ( 2 ) O ( 2 ) on glucose uptake in a human leukemic megakaryocytic cell line , M 07 ; ( 2 ) investigate the changes in kinetic parameters of the transport activity induced by these stimuli ; and ( 3 ) evaluate the effect of genistein , a tyrosine kinase inhibitor , on the glucose uptake activation by the cited agents . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
METHODS : Murine bone marrow derived Lin c kit+ HPCs were isolated by a cell sorter and cultured in the presence of granulocyte macrophage colony stimulating factor ( GM CSF ) , stem cell factor ( SCF ) , tumor necrosis factor alpha ( TNF alpha ) and TGF beta 1 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
DCs could be induced either from short or long term cultured CD34+ cells after switching the cytokines from Flk 2 / Flt 3 ligand , stem cell factor ( SCF ) , thrombopoietin ( TPO ) to granulocyte macrophage colony stimulating factor ( GM CSF ) and interleukin 4 ( IL 4 ) ( immature type ) plus tumor necrosis factor ( TNF ) alpha with stimulation by CD40L transfectant ( mature type ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We found that several HGFs , including granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) , steel factor ( SF ) , and thrombopoietin ( TPO ) induce a rapid increase in reactive oxygen species ( ROS ) in quiescent cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The combination of thermal injury plus PAO 1 infection enhanced the expression of several pro inflammatory and haematopoietic cytokines [ stem cell factor ( SCF ) , leukocyte inhibitory factor ( LIF ) , IL 6 and TNF alpha ] ; induced the expression of granulocyte macrophage colony stimulating factor ( GM CSF ) and G CSF by 5 h and the expression of additional cytokines , including TGF beta , TNF beta , lymphotoxin beta ( LT beta ) , interferon gamma ( IFN gamma ) , and IFN beta by 40 h post burn / infection . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : In severely hypoxic ( 0 . 9 % O ( 2 ) ) murine bone marrow cell cultures containing stem cell factor , interleukin 6 , and granulocyte colony stimulating factor , pre CFC maintenance was characterized by the culture repopulating ability assay , an in vitro analogue of the marrow repopulating ability assay . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Cord blood CD 34 ( + ) cells were cultured for 3 , 7 or 10 d with stem cell factor , Flt 3 , thrombopoietin , interleukin 3 ( IL 3 ) , IL 6 and granulocyte colony stimulating factor , all at 10 ng / ml in serum replete conditions . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Immortalized human bone marrow endothelial cells ( BMEC ) transfected with an expression vector carrying cDNA encoding the human telomerase reverse transcriptase ( hTERT ) and human umbilical vein endothelial cells ( HUVEC ) were transfected with combinations of adenovectors expressing murine c kit ligand ( KL ) , human thrombopoietin ( TPO ) , human Flt 3 ligand ( FL ) , and human granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Colony formation from normal bone marrow was not increased if stromal layers were incubated with 100 ng / mL granulocyte colony stimulating factor ( G CSF ) or stem cell factor ( SCF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
To this end , we compared expression of RNA encoding the receptors for interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , IL 6 , leukemia inhibitory factor ( LIF ) and stem cell factor ( SCF ) in populations of primitive hematopoietic progenitors ( lineage marker negative , Lin ( ) , and Lin ( ) c Kit ( + ) ) by RT PCR and in situ RT PCR . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Among various combinations , the combination of c kit ligand , flt 3 ligand , c mpl ligand ( TPO ) , and interleukin ( IL ) 4 most potently generated the number of CD1a+CD14 DCs in cultures containing granulocyte macrophage colony stimulating factor ( GM CSF ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The aim of this study was to identify signal transduction pathways activated by erythropoietin ( EpO ) and erythropoietin co stimulatory factors ( kit ligand ) , insulin like growth factor , thrombopoietin , interleukin 3 and granulocyte macrophage colony stimulating factor ) in normal human bone marrow CD 34 ( + ) cells and d 11 erythroid burst forming unit derived glycophorin+ cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Equivalent yields of MP were obtained using TPO and one of IL 1 , IL 3 , granulocyte macrophage colony stimulating factor or SCF , suggesting that large cytokine combinations are not necessary for this procedure . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Samples of the patient ' s bone marrow showed trilineage hypoplasia with absence of myeloid , erythroid , and megakaryocyte progenitor cells but with elevated endogenous levels of erythropoietin , granulocyte colony stimulating factor , and stem cell factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Purified CD34+ cells from CB and PB were cultured on murine stroma cell line MS 5 with stem cell factor and granulocyte colony stimulating factor for 6 weeks . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Tissue sections were tested using immunohistochemistry for the expression of keratinocyte derived cytokines with stimulating activity , such as granulocyte monocyte colony stimulating factor ( GM CSF ) , basic fibroblastic growth factor ( bFGF ) , and stem cell factor ( SCF ) or with inhibiting activity , such as interleukin 6 ( IL 6 ) and tumour necrosis factor alpha ( TNF alpha ) on melanocytes . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
PATIENTS AND METHODS : Unselected peripheral blood progenitor cells ( PBPCs ) from 43 breast cancer patients were seeded into gas permeable bags containing serum free media , granulocyte colony stimulating factor , stem cell factor , and pegylated megakaryocyte growth and development factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In this assay , unfractionated mononuclear cells from apheresis donors are incubated in nonpyrogenic plastic bags for 7 days in the presence of culture medium either containing fetal calf serum or human plasma , granulocyte colony stimulating factor , and stem cell factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Synergistic effects with the haematopoietic cytokines stem cell factor ( SCF ) and granulocyte macrophage colony stimulating factor ( GM CSF ) were observed . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Furthermore , when compared with WT , hematopoietic precursor cells from PLSCR 1 ( / ) mice showed defective colony formation and impaired differentiation to mature granulocytes as stimulated by stem cell factor and granulocyte colony stimulating factor ( G CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Lin ( ) Sca 1 ( + ) HPC cultured 9 days in 100 ng / mL stem cell factor ( SCF ) , 20 ng / mL interleukin 3 ( IL 3 ) , 50 ng / mL monocyte colony stimulating factor ( M CSF ) , 5 ng / mL granulocyte monocyte colony stimulating factor ( GM CSF ) , and 25 ng / mL FLT 3 ligand ( FLT 3 L ) proliferate 387 fold and differentiate into DC precursors . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
MATERIAL AND METHODS : CD 34 ( + ) cells were selected from CB products and cultured in a two step procedure in the presence of stem cell factor , megakaryocyte growth and differentiation factor , and granulocyte colony stimulating factor for 14 days . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Cytokine stimulated stem cell factor ( SCF ) and granulocyte colony stimulating factor ( G CSF ) purified sca ( + ) / lin ( ) cells of C57BL / 6 ( H 2b , 1E ( + ) ) background were injected into MHC mismatched BALB / c ( H 2d , 1E ( ) ) fetal mouse recipients at day 13 of gestation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Recipient mice were treated for 1 or 7 days with growth factors ( stem cell factor [ SCF ] plus interleukin 11 [ IL 11 ] , SCF plus Flt 3 ligand [ FL ] , or granulocyte colony stimulating factor [ G CSF ] ) prior to low dose TBI ( 4 Gy ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Using bone marrow cells as the source of precursors we generated `` myeloid type ' ' DCs from the adherent population using granulocyte macrophage colony stimulating factor ( GM CSF ) , IL 4 and Flt 3L or `` lymphoid type ' ' DCs from the non adherent population using cytokines which included IL 7 , IL 3 , SCF and TNFalpha . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Retroviral mediated expression of DokR in bone marrow cells dramatically inhibited their capacity to form colonies in vitro in response to the cytokines macrophage colony stimulating factor and stem cell factor , whereas responses to interleukin 3 and granulocyte macrophage colony stimulating factor were only weakly affected . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Macrophage colony stimulating factor , granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) and interleukin 7 ( IL 7 ) are potent cytokines belonging to the same structual class that may affect function , growth and apoptosis both in the heart and other organs . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
TGD also enhanced secretion of granulocyte monocyte colony stimulating factor and stem cell factor but did not affect the production of erythropoietin , interleukin 3 , growth hormone , or of full length TPO . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Using a nuclease protection assay we investigated mRNA expression of stem cell factor ( SCF ) , a crucial survival factor for mast cells , and the macrophage growth factors macrophage colony stimulating factor ( M CSF ) and granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Moreover , OGP 10 14 preserves stem cells self renewal potential in long term culture ( LTC ) initiating cells and acts directly on CD34+ enriched cells or by increasing activity of stem cell factor ( SCF ) and granulocyte megakaryocyte colony stimulating factor . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Using in vitro progenitor assays , serum free in vitro cultures , and the nonobese diabetic / severe combined immune deficient ( NOD / SCID ) ecotropic murine virus knockout xenotransplantation model to detect human SCID repopulating cells ( SRCs ) with multilineage reconstituting function , we have characterized and compared purified subpopulations harvested from the peripheral blood ( PB ) of patients receiving granulocyte colony stimulating factor ( G CSF ) alone or in combination with stem cell factor ( SCF ) . ^^^ Functional analysis of human hematopoietic repopulating cells mobilized with granulocyte colony stimulating factor alone versus granulocyte colony stimulating factor in combination with stem cell factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The synergy between stem cell factor ( SCF ) and granulocyte colony stimulating factor ( G CSF ) : molecular basis and clinical relevance . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Administration of granulocyte colony stimulating factor and stem cell factor resulted in mobilization of transduced bone marrow cells detectable as mature granulocyte progeny which expressed the GFP gene , suggesting that transduced progenitor cells in bone marrow could be mobilized into the peripheral blood and differentiated into granulocytes . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Concentrations of the cytokines known to induce CD 16 ( including transforming growth factor beta 1 [ TGFbeta 1 ] , macrophage colony stimulating factor [ M CSF ] , and interleukin 10 [ IL 10 ] ) and concentrations of the soluble form of CD 14 ( sCD 14 ) in plasma and synovial fluid ( SF ) samples were measured by enzyme linked immunosorbent assay . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Bone marrow was aspirated from the ilium and / or tuber ischiae after administration of recombinant human stem cell factor ( SCF ) and granulocyte colony stimulating factor ( G CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
This study investigated early ex vivo expansion kinetics of UCB AC133+ cells ( 2 4 10 10 ( 4 ) / ml ) , mononuclear cells ( MNC , 1 2 10 10 ( 6 ) / ml ) and AC133negative cells ( AC 133 ( neg ) , 2 4 10 10 ( 4 ) / ml ) in stroma free 8 d liquid culture ( fetal bovine serum supplemented Iscove ' s modified Dulbecco ' s medium ( IMDM ) with either ' K36EG ' [ c Kit ligand , interleukin 3 ( IL 3 ) , IL 6 , erythropoietin , granulocyte colony stimulating factor ] or ' TPOFL ' ( thrombopoietin , Flt 3 ligand ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The usage of cytokines was classified into two groups : group A ( stem cell factor , granulocyte / macrophage colony stimulating factor , tumor necrosis factor alpha and interleukin 4 ) and group B ( granulocyte / macrophage colony stimulating factor , tumor necrosis factor alpha and interleukin 4 ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Synergism between stem cell factor ( SCF ) and granulocyte macrophage colony stimulating factor ( GM CSF ) has been shown to be essential for hematopoietic cell proliferation . ^^^ Synergism between stem cell factor and granulocyte macrophage colony stimulating factor on cell proliferation by induction of cyclins . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In most nonhuman primate studies , hematopoietic stem cells ( HSCs ) collected from the peripheral blood or bone marrow after administration of granulocyte colony stimulating factor ( G CSF ) + stem cell factor ( SCF ) have been used as targets , but this cytokine combination is not generally available for clinical use , and the optimum target cell population has not been systematically studied . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
A fraction of each patient ' s CB allograft was CD 34 selected and cultured ex vivo for 10 days prior to transplantation in defined media with stem cell factor , granulocyte colony stimulating factor , and megakaryocyte growth and differentiation factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Although Shiga toxin 2 alone induced colonies mainly composed of macrophages , it induced colonies mainly consisting of granulocytes when combined with physiological doses of interleukin 1beta , granulocyte colony stimulating factor , or stem cell factor with interleukin 3 . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CD34+ cells from cord blood ( CB ) , bone marrow ( BM ) , or peripheral blood ( PB ) were expanded in a medium containing stem cell factor ( SCF ) , flt 3 ligand ( Flt3L ) and thrombopoietin ( TPO ) with or without HESS 5 , a murine BM stromal cell line , for 2 weeks ( the first expansion step ) , then differentiated to DC in a medium containing granulocyte macrophage colony stimulating factor ( GM CSF ) , flt 3 ligand ( Flt3L ) , stem cell factor ( SCF ) , tumor necrosis factor alpha ( TNF alpha ) , IL 4 , and lipopolysaccharide ( LPS ) for 9 days ( the second differentiation step ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
All 4 AML samples showed evidence of autocrine production of 2 or more of SF , FL , IL 3 , and granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Patients ( n = 69 ) with multiple myeloma undergoing peripheral blood stem cell collection ( PBSC ) were treated with cyclophosphamide and a combination of recombinant methionyl human granulocyte colony stimulating factor ( r metHuG CSF , filgrastim ) and recombinant methionyl human stem cell factor ( r metHuSCF , ancestim ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Based on granulocyte colony stimulating factor ( G CSF ) and erythropoietin ( EPO ) alone serve as a baseline with which to compare the effects of the combinations with stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) or granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The ability of acute myeloid leukaemia ( AML ) cells to acquire dendritic cell ( DC ) like characteristics in vitro with a rapid culture method based either on the phorbol ester PMA or calcium ionophores has been studied in comparison to conventional AML DC cultures with the cytokines granulocyte macrophage colony stimulating factor ( GM CSF ) , tumour necrosis factor alpha ( TNF alpha ) , interleukin 3 ( IL 3 ) , SCF , FLT 3 L and IL 4 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We replaced granulocyte macrophage colony stimulating factor with macrophage colony stimulating factor from a serum free culture containing granulocyte macrophage colony stimulating factor , stem cell factor , Flt 3 ligand , tumor necrosis factor alpha , and transforming growth factor beta 1 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
These findings were positively correlated with the up regulation of cytokine mRNA expression such as granulocyte colony stimulating factor ( GM CSF ) , erythropoietin ( EPO ) , thrombopoietin ( TPO ) , stem cell factor ( SCF ) , and c Kit . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
STUDY DESIGN AND METHODS : The effects of three different mobilization regimens stem cell factor ( SCF ) plus granulocyte colony stimulating factor ( G CSF ) plus cyclophosphamide ( CCP ) , G CSF alone , and G CSF plus CCP on the composition of LPs from patients with nonhematologic PBPC malignancies compared to LPs from G CSF mobilized healthy donors and normal marrow ( BM ) samples were analyzed . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We previously reported that chronic myelogenous leukemia ( CML ) primitive granulocyte monocyte ( GM ) progenitors have a greatly reduced requirement for kit ligand ( KL ) to achieve optimal growth with granulocyte colony stimulating factor ( G CSF ) + granulocyte monocyte colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Compared with prestimulated ( stem cell factor , granulocyte colony stimulating factor , granulocyte macrophage colony stimulating factor , interleukin 3 , interleukin 6 , erythropoietin , and monoclonal antibody to transforming growth factor beta ) , transfection of thawed nonstimulated PBSCs was equally efficient , with a median transfection rate of 3 . 7 % , transfection efficiency of 0 . 8 % , and survival of 19 . 5 % ( n = 5 ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
METHODS : Purified 1 10 10 ( 4 ) circulating CD34+ cells isolated from rHuEPO hyporesponsive HD patients ( EPO H ; n = 10 ) , rHuEPO responsive non HD patients with CRF ( EPO R ; n = 8 ) , nonanemic HD patients without rHuEPO therapy ( EPO W / O ; n = 10 ) , and healthy volunteer controls ( CON ; n = 10 ) were subjected to a methylcellulose culture system supplemented with rHuEPO , recombinant human interleukin 3 ( IL 3 ) , recombinant human stem cell factor ( SCF ) , and recombinant human granulocyte macrophage colony stimulating factor ( GM CSF ) for 14 days . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Successful mobilization of peripheral blood stem cells after addition of ancestim ( stem cell factor ) in patients who had failed a prior mobilization with filgrastim ( granulocyte colony stimulating factor ) alone or with chemotherapy plus filgrastim . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Growth factors contributing to it , namely interleukin ( IL ) 6 , IL 11 , stem cell factor , leukemia inhibitory factor , granulocyte colony stimulating factor , thrombopoietin ( TPO ) , and the regulation of megakaryocytopoiesis during the inflammatory cascade are reviewed . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
METHODS : Tissue sections from patients with different types of urticaria and healthy controls were studied for the immunohistochemical expression of known mast cell growth factors ( stem cell factor , SCF ; nerve growth factor , NGF ) , of the inhibitory granulocyte macrophage colony stimulating factor ( GM CSF ) , and of the corresponding receptors , using the alkaline phosphatase antialkaline phosphatase technique . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
RT PCR analysis showed that HYMEQ 5 produced FL , SCF , interleukin 6 , and macrophage colony stimulating factor ( M CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The adherent cells were differentiated into DCs within 2 weeks by adding granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 4 ( IL 4 ) , c kit ligand and tumour necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CD 34 ( + ) cells purified from ten CBs by immunomagnetic selection were cultured in serum free essential media in the presence of a combination of the several cytokines including EPO , thrombopoietin , flt 3 ligand ( FL ) , stem cell factor ( SCF ) , granulocyte colony stimulating factor , interleukin ( IL ) 3 and / or IL 11 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
When vibrissal follicles of adult mice were cultured in the presence of stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , IL 7 , granulocyte macrophage colony stimulating factor , and Flt 3 ligand , CD45+ / lineage / c kit+ / FcepsilonRI+ cells became detectable on the outgrowing fibroblasts in 10 days and expanded progressively thereafter . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Several hematopoietic growth factors including interleukin 3 ( IL 3 ) , IL 6 , granulocyte macrophage colony stimulating factors ( GM CSF ) , granulocyte colony stimulating factor ( G CSF ) , and stem cell factor ( SCF ) have been reported to be positive regulators of granulopoiesis and act at different stages of myeloid cell development . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Alteration of spontaneous proliferation by at least one mediator was detected in five of 38 patients ; whereas , altered cytokine ( Flt 3 ligand , granulocyte macrophage colony stimulating factor , stem cell factor ) dependant proliferation was observed for 10 patients . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Successful stem cell mobilization with stem cell factor and granulocyte colony stimulating factor in patients with solid tumors failing conventional mobilization with chemotherapy and G CSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Enhanced antileukemic activity of allogeneic peripheral blood progenitor cell transplants following donor treatment with the combination of granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) in a murine transplantation model . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CD 14 ( + ) monocytes and CD 34 ( + ) stem cells were isolated from human umbilical cord blood and were induced to differentiate into dendritic cells using 100 ng / mL granulocyte macrophage colony stimulating factor ( GM CSF ) , 25 ng / mL IL 4 , 2 . 5 ng / mL tumor necrosis factor alpha ( TNF alpha ) , 100 ng / mL GM CSF , 25 ng / mL stem cell factor , and 2 . 5 ng / mL TNF alpha , respectively . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Recombinant porcine ( rp ) SCF and granulocyte macrophage colony stimulating factor ( GM CSF ) were expressed in the mammalian HEK 293 cell based expression system . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
RECENT FINDINGS : Several studies have recently shown that fibroblasts can modulate the functions of mast cells and eosinophils through the membrane form of stem cell factor and granulocyte macrophage colony stimulating factor , respectively . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We examined the effects of thrombopoietin ( TPO ) in combination with stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , and granulocyte colony stimulating factor ( G CSF ) on the proliferation and differentiation of human neutrophils . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The FLCM was assayed for the presence of four principal colony stimulating cytokines , namely stem cell factor ( SCF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) , and erythropoietin ( Epo ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
DESIGN AND METHODS : Cord blood mononuclear cells were co cultured in a liquid culture system containing the hematopoietic cytokines interleukin ( IL ) 1 , IL 3 , IL 6 , stem cell factor ( SCF ) , granulocyte monocyte colony stimulating factor ( GM CSF ) and G CSF for a total of 20 days with or without depletion of NK cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CD34+ stem cells were isolated from umbilical cord blood and cultured using granulocyte macrophage colony stimulating factor , stem cell factor , and varying concentrations of TNFalpha . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Most gene transfer studies have used peripheral blood or marrow repopulating cells collected after administration of granulocyte colony stimulating factor and stem cell factor ( G CSF / SCF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
To investigate the relationship between c kit expression and cell cycle regulation by endogenous transforming growth factor beta ( TGF beta ) in human bone marrow hematopoietic progenitor cells , CD34+ CD 38 c kit ( low / ) and CD34+ CD 38 c kit ( high ) populations were cultured in stem cell factor , thrombopoietin , interleukin 3 ( IL 3 ) , IL 6 , granulocyte colony stimulating factor , granulocyte / macrophage colony stimulating factor and anti TGF beta , and analyzed for cell cycle status . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
This study investigated whether mobilization of polymorphonuclear cells ( PMN ) by granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) enhances immigration of opioid containing PMN and peripheral opioid analgesia in rats with Freund complete adjuvant induced hind paw inflammation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Therefore , we examined the expression of receptors for stem cell factor ( SCF ) , interleukin 6 ( IL 6 ) , IL 3 , granulocyte colony stimulating factor ( G CSF ) and IL 7 on the CD 34 ( + ) cells of cord blood , mobilized peripheral blood and bone marrow . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Fresh leukaemic cells from five patients with acute myeloid leukaemia ( AML ) were isolated and retrovirally transduced with OX40L during the culture with a combination of cytokines from stem cell factor , fms like tyrosine kinase ( Flt ) 3 ligand , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 4 ( IL 4 ) and TNF alpha . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
These include endothelin ( ET ) 1 , granulocyte macrophage colony stimulating factor , membrane type stem cell factor ( SCF ) and growth related oncogene alpha for interactions between keratinocytes and melanocytes , and hepatocyte growth factor and soluble type SCF for interactions between fibroblasts and melanocytes . ^^^ These are ET 1 / ET B receptor as well as membrane type SCF / c KIT for ultraviolet B melanosis , granulocyte macrophage colony stimulating factor for ultraviolet A melanosis , ET 1 / ET B receptor as well as membrane type SCF for lentigo senilis , growth related oncogene alpha for Riehl ' s melanosis , sphingosylphosphorylcholine for hyperpigmentation in atopic dermatitis , ET 1 for seborrhoeic keratosis , soluble type SCF as well as hepatocyte growth factor for dermatofibroma and caf au lait macules , and c KIT for vitiligo vulgaris . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Human umbilical cord blood CD14+ monocytes and CD34+ stem cells were induced to differentiate into dendritic cells using 100 ng / mL granulocyte macrophage colony stimulating factor ( GM CSF ) , 25 ng / mL interleukin ( 2 ) 4 , 2 . 5 ng / mL tumor necrosis factor alpha ( TNF alpha ) and 100 ng / mL GM CSF , 25 ng / mL stem cell factor , and 2 . 5 ng / mL TNF alpha , respectively . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
RESULTS : We found that growth factors such as epidermal growth factor , transforming growth factor alpha , amphiregulin , heparin binding epidermal growth factor like growth factor , beta cellulin , insulin like growth factor 1 , insulin like growth factor 2 and stem cell factor stimulated directional ( chemotactic ) and / or random ( chemokinetic ) motility in all mesothelioma cell lines tested , whereas none of acidic fibroblast growth factor , basic fibroblast growth factor , granulocyte macrophage colony stimulating factor or interleukin 6 induced migration in the same mesothelioma cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Synergistic growth of stem cell factor and granulocyte macrophage colony stimulating factor involves kinase dependent and independent contributions from c Kit . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Mice with targeted deletion of PLSCR 1 display perinatal granulocytopenia due to defective response of hematopoietic precursors to granulocyte colony stimulating factor and stem cell factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Several cytokines , especially stem cell factor ( SCF ) and FLT 3 ligand ( FL ) , have been identified as essential to produce large numbers of myeloid precursors and even to increase DC yield obtained by the action of granulocyte macrophage colony stimulating factor ( GM CSF ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We report the results of experiments with CD 34 ( + ) cells from cord blood ( CB , n = 13 ) , bone marrow ( BM , n = 4 ) , and mobilized peripheral blood stem cells ( PBSC , n = 5 ) using two combinations of cytokines : ( A ) granulocyte colony stimulating factor ( G CSF ) , interleukin 3 ( IL 3 ) , interleukin 6 ( IL 6 ) , stem cell factor ( SCF ) , erythropoietin ( EPO ) , insulin like growth factor 1 ( IGF 1 ) , basic fibroblast growth factor ( FGF b ) and ( B ) combination A plus FLT 3 ligand ( FL ) and megakaryocyte growth and development factor ( PEG rhMGDF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Noncontact HUBEC cultures and the addition of thrombopoietin , stem cell factor ( SCF ) , and macrophage colony stimulating factor 1 receptor ( Fms ) like tyrosine kinase 3 ( Flt 3 ) ligand supported further increases in CD 34 ( + ) CD 38 cells ( 6 . 4 fold and 13 . 1 fold ) , which correlated with significant increases in SRC activity . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
LTA altered either cytokine ( granulocyte macrophage colony stimulating factor + stem cell factor + IL 3 ) dependent proliferation or the release of IL1beta / IL8 for 23 patients ; the effects were divergent but increased proliferation / cytokine levels were most commonly observed . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
This study has compared the expression of endothelin ( ET ) 1 , the ET 1 receptor ( ET ( B ) R ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , stem cell factor ( SCF ) , the SCF receptor ( KIT protein ) , tyrosinase , and S 100 alpha between lesional and non lesional vitiligo epidermis . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In order to permit cellular differentiation towards the myeloid lineage , PBPCs were cultured for 14 21 days in a liquid , serum free medium supplemented with interleukin 1 ( IL 1 ) , IL 3 , IL 6 , granulocyte macrophage colony stimulating factor , granulocyte colony stimulating factor and stem cell factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The nature of UCBC was freshly determined and then UCBC were cultured for 1 , 2 , 3 and 4 weeks with granulocyte monocyte colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) , recombinant human stem cell factor ( SCF ) and EPO . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In addition , this fraction differentially regulated liver and lung cytokine mRNA levels , resulting in significant increases in message for hematopoietic cytokines [ granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) ] . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We cultured cord blood CD34+ cells in RPMI 1640 with 10 % human AB plasma , stem cell factor ( days 1 6 ) , granulocyte macrophage colony stimulating factor ( days 1 18 ) , interleukin 4 ( days 6 18 ) and varying schedules of TNF alpha ( 0 144 h after thawing ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The concentrations of interleukin 6 , stem cell factor , granulocyte monocyte colony stimulating factor , granulocyte colony stimulating factor , erythropoietin , and growth hormone ( GH ) were evaluated before and in the eight hours after exercise . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Similar to endothelial cells , myxoma cells did not produce stem cell factor , granulocyte colony stimulating factor , hepatocyte growth factor , or ET 3 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The cells were cultured in STEM PRO 34 liquid medium , supplemented with rHuEPO , IL 3 , stem cell factor , and granulocyte macrophage colony stimulating factor for 7 d and then transferred to a semisolid methylcellulose culture medium for performing burst forming unit erythroid ( BFU E ) colony assay . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
METHODS : CD34+ cells purified from the bone marrow of 13 patients with active RA and 9 control subjects ( 7 osteoarthritis [ OA ] patients and 2 healthy individuals ) were cultured in the presence of stem cell factor ( 10 ng / ml ) and granulocyte macrophage colony stimulating factor ( 1 ng / ml ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In addition , these infected cells were differentiated into immature and mature dendritic cells ( DCs ) using cytokine induction with recombinant human granulocyte macrophage colony stimulating factor ( rhGm CSF ) , recombinant human tumor necrosis factor ( rhTNF alpha ) and recombinant human stem cell factor ( rhSCF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
BM mononuclear cells from 3 dogs with melanoma and from 1 healthy dog were cultured for 12 days in media supplemented with recombinant human granulocyte macrophage colony stimulating factor , stem cell factor , tumor necrosis factor , and Flt 3 ligand . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
This study investigated whether the enhanced availability of hematopoietic stem cells , induced by stem cell factor and granulocyte colony stimulating factor , to the injured kidney provides an adequate strategy for stem cell based therapy to counteract renal ischemia / reperfusion injury . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
A novel human stem cell factor ( SCF ) / macrophage colony stimulating factor ( M CSF ) fusion protein gene was constructed , in which the coding regions of human SCF cDNA ( 1 165aa ) and the truncated M CSF cDNA ( 1 149aa ) were connected by a linker sequence encoding a short peptide GGGGSGGGGSGG . ^^^ Expression of a novel recombinant stem cell factor / macrophage colony stimulating factor fusion protein in baculovirus infected insect cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Late MSCs also exhibited attenuated synthesis of the hematopoietic cytokines granulocyte colony stimulating factor ( G CSF ) , leukemia inhibitory factor ( LIF ) , and stem cell factor ( SCF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Successful mobilization of peripheral blood stem cells using recombinant human stem cell factor in heavily pretreated patients who have failed a previous attempt with a granulocyte colony stimulating factor based regimen . ^^^ To assess the efficacy of recombinant human stem cell factor ( rHuSCF ) , 48 patients who had failed to mobilize > 2 . 0 10 10 ( 6 ) CD34+ cells / kg with granulocyte colony stimulating factor ( G CSF ) ( 10 microg / kg twice daily ) with , or without , concomitant chemotherapy ( G CSF based regimen ) , were remobilized with the addition of rHuSCF ( 20 microg / kg / day ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Subcutaneous administration of granulocyte colony stimulating factor and stem cell factor ameliorates the outcome of acute myocardial infarction . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Monocytes from healthy donors were differentiated in vitro into DC with granulocyte macrophage colony stimulating factor ( GM CSF ) and interleukin ( IL ) 4 , in the absence or presence of soluble factors ( SF ) from SCC or AD cell lines . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) and macrophage colony stimulating factor ( M CSF ) can act in synergistic way to promote the growth of mononuclear phagocytes . ^^^ Design of recombinant stem cell factor macrophage colony stimulating factor fusion proteins and their biological activity in vitro . ^^^ Stem cell factor ( SCF ) and macrophage colony stimulating factor ( M CSF ) can act in synergistic way to promote the growth of mononuclear phagocytes . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
OBJECTIVES : We investigated whether granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) could promote myocardial regeneration after coronary artery occlusion and improve left ventricular ( LV ) function . ^^^ Granulocyte colony stimulating factor and stem cell factor improve contractile reserve of the infarcted left ventricle independent of restoring muscle mass . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
To evaluate the OP 9 capability to sustain long term hematopoiesis , we reported the expansion of granulocyte colony stimulating factor ( G CSF ) mobilized human peripheral blood ( PB ) CD 34 ( + ) cells in co culture with murine OP 9 and MS 5 stromal cells , either transfected with various combinations of adenovectors ( Ad ) expressing c kit ligand ( KL ) ( either soluble or transmembrane form ) , thrombopoietin ( TPO ) , flt 3 / flk2 ligand ( FL ) , and granulocyte macrophage ( GM ) CSF or with weekly addition of these cytokines . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We studied the effect of two different cytokine combinations kit ligand ( KL ) , interleukin 3 ( IL 3 ) , and Flt 3 ligand supplemented with thrombopoietin and IL 11 ( combination 1 ) or granulocyte / macrophage colony stimulating factor ( GM CSF ) and G CSF ( combination 2 ) for their ability to affect speed and extent of engraftment using limited numbers ( 5 10 10 ( 4 ) ) of murine bone marrow ( BM ) light density ( LD ) cells or their progeny expanded ex vivo in the presence one or the other cytokine combination for 6 days . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Ex vivo culture was performed in medium supplemented with granulocyte colony stimulating factor , stem cell factor and either thrombopoietin or megakaryocyte growth and differentiation factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Granulocyte colony stimulating factor ( G CSF ) , alone or in combination with stem cell factor ( SCF ) , can improve hemodynamic cardiac function after myocardial infarction . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Immature DCs are generated from human cord blood CD34+ hematopoietic stem / progenitor cells that were cultured in the presence of stem cell factor , Fms like tyrosine kinase 3 ligand , granulocyte macrophage colony stimulating factor , and transform growth factor beta 1 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Since primitive CML cells differentiate more rapidly in vitro than their normal counterparts and are also preferentially killed by mafosfamide and imatinib , we examined the purging effectiveness on CD 34 ( + ) CML cells using a strategy that combines a brief exposure to imatinib ( 0 . 5 1 . 0 microM for 72 h ) and then mafosfamide ( 30 90 microg / ml for 30 min ) followed by 2 weeks in culture with cytokines ( 100 ng / ml each of stem cell factor , granulocyte colony stimulating factor and thrombopoietin ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
BACKGROUND : Hematopoietic cytokines , granulocyte colony stimulating factor ( G CSF ) , and stem cell factor ( SCF ) were reported to show a neuroprotective effect or to support neurogenesis . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
OBJECTIVE : The aims of this study were , first , to determine if granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) improved left ventricular function in the setting of a reperfusion model of myocardial infarction ( MI ) and , second , to evaluate the effects of G CSF / SCF on cellular repair and , in particular , the fate of bone marrow cells homing to the site of tissue injury . ^^^ Granulocyte colony stimulating factor and stem cell factor improve endogenous repair after myocardial infarction . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Silencing of NFkappaB 1 by small interfering RNA abrogated the capacity of RA bone marrow CD34+ cells to differentiate into fibroblast like cells and to produce MMP 1 and vascular endothelial growth factor upon stimulation with stem cell factor , granulocyte macrophage colony stimulating factor and TNF alpha without influencing their viability and capacity to produce beta 2 microglobulin . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Most large animal studies have utilized peripheral blood or marrow derived cells collected after administration of granulocyte colony stimulating factor ( G SCF ) and stem cell factor ( SCF ) ; however , SCF is unavailable for clinical use in the United States and the European Union . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Wild type ( WT ) mice underwent a 30 minute coronary occlusion followed by reperfusion and received vehicle , granulocyte colony stimulating factor ( G CSF ) +Flt 3 ligand ( FL ) , G CSF+stem cell factor ( SCF ) , or G CSF alone starting 4 hours after reperfusion . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Soluble Kit ligand ( sKitL ) , thrombopoietin ( TPO , encoded by Thpo ) and , to a lesser extent , erythropoietin ( EPO ) and granulocyte macrophage colony stimulating factor ( GM CSF ) induced the release of SDF 1 from platelets , enhancing neovascularization through mobilization of CXCR4+ VEGFR1+ hemangiocytes . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
These conditions include : the combinations and concentrations of hematopoietic growth factors ( stem cell factor [ SCF ] , granulocyte macrophage colony stimulating factor [ GM CSF ] , interleukin [ IL ] 3 , thrombopoietin [ Tpo ] , IL 6 and Fms like tyrosine kinase 3 ligand [ Flt 3L ] ) , the duration of culture , and the effect of serum supplementation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
On the basis of changes in protein expression , we identified 6 cytokines that accurately discriminate between individuals on the basis of adverse event status : granulocyte colony stimulating factor , stem cell factor , monokine induced by interferon gamma ( CXCL 9 ) , intercellular adhesion molecule 1 , eotaxin , and tissue inhibitor of metalloproteinases 2 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The messenger RNA expressions of LARC ( liver and activation regulated chemokine ) , GMCSF ( granulocyte macrophage colony stimulating factor ) , epiregulin , ICAM 1 ( intercellular adhesion molecule 1 ) , and TGFA ( transforming growth factor alpha ) were more strongly up regulated by IL 1alpha and / or IL 1beta in MECF than in SF , suggesting that these fibroblasts derived from different tissues retained their typical gene expression profiles . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CSFs included stem cell factor ( SCF ) , erythropoietin ( EPO ) , granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , macrophage colony stimulating factor ( M CSF , CSF 1 ) , and thrombopoietin ( TPO ) , or analogues of these . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
OBJECTIVE : Cytokine treatment with granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and stem cell factor ( SCF ) is a mainstay of current and future clinical and research protocols for peripheral blood stem cell mobilization , therapeutic care after hematopoietic stem cell transplantation ( HSCT ) , and ex vivo hematopoietic stem and progenitor cell ( HSC / HPC ) expansion . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) are potential new therapies to ameliorate post myocardial infarction ( post MI ) remodeling , as they enhance endogenous cardiac repair mechanisms and decrease cardiomyocyte apoptosis . ^^^ Aging impairs the beneficial effect of granulocyte colony stimulating factor and stem cell factor on post myocardial infarction remodeling . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
While thrombopoietin ( TPO ) , granulocyte colony stimulating factor ( G CSF ) or stem cell factor ( SCF ) were not able to increase cAMP levels , the antiapoptotic activity exerted by these growth factors was blocked by inhibition of the adenylate cyclase and synergized by BIMPS . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
This cell line , designated OCI / AML1a , does not respond to GM CSF , interleukin 3 ( IL 3 ) , IL 1 or stem cell factor as well as G CSF . ^^^ This cell line TMD 2 does not respond to G CSF , GM CSF , IL 1 , or stem cell factor and anti IL 3 antibody blocks the stimulatory effect of IL 3 on these cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Gonarthrosis in 11 human T cell leukemia virus 1 ( HTLV 1 ) carriers were studied for sera , synovial fluids ( SF ) and cerebrospinal fluids ( CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Using optimal concentrations of colony stimulating factors ( CSF ) without added SCF approximately 2 . 5 % of cells formed colonies . ^^^ SCF also had CSF activity on this population , up to 0 . 5 % of cells forming small colonies in response to SCF alone . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Analysis of the NK cell supernatants using factor dependent myeloid progenitor cell lines showed that the NK cells were producing G CSF and growth promoting activity that could not be attributed to IL 1 , IL 3 , IL 4 , IL 5 , IL 6 , GM CSF , G CSF , macrophage CSF ( M CSF ) , or stem cell factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Synovial fluids ( SF ) from patients with osteoarthritis ( OA ) and rheumatoid arthritis ( RA ) and various other arthritides were examined for the presence of colony stimulating factors ( CSF ) . ^^^ CSF was found in 7 of 13 ( 54 % ) SF from OA patients and in 8 of 12 ( 67 % ) SF from RA patients . ^^^ While CSF was found in both inflammatory and noninflammatory effusions , significantly greater numbers of colonies were stimulated by RA SF than by OA SF and in general greater numbers of colonies correlated with higher SF leukocyte counts . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We describe a high performance liquid chromatographic method , using electrochemical detection , for the determination of pirprofen in cerebrospinal fluid ( CSF ) , plasma , and synovial fluid ( SF ) . ^^^ The detection limit was 0 . 1 ng / mL for CSF , and 1 ng / mL for plasma and SF , with a recovery greater than 96 % and intraday coefficient of variation less than 5 % in all cases . ^^^ The main advantages of this method include high specificity and sensitivity which allow the analysis of CSF and the use of small volumes of plasma and SF . ^^^ The application of the method for the analysis of plasma and SF samples and the kinetic profile in CSF are shown . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We have investigated the influence of different hematopoietic growth factors , including granulocyte colony stimulating factor ( G CSF ) , stem cell factor ( SCF ) , Flt 3 ligand ( Flt 3L ) and thrombopoietin ( TPO ) , on the course of relapsing experimental autoimmune encephalomyelitis , a mouse model of multiple sclerosis . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
A liquid culture technique was used to study 23 synovial fluids ( SF ) ( 21 from inflammatory joint diseases and 2 noninflammatory SF ) and supernatants of two cultured rheumatoid arthritis ( RA ) synovial tissues for colony stimulating factor ( CSF ) . ^^^ CSF activity was observed in inflammatory SF , with tritiated thymidine uptake of 3 , 501 + / 1 , 140 cpm in the presence of RA samples ( n = 15 ) compared to 1 , 985 + / 628 for non RA inflammatory SF ( n = 7 ) ( P less than 0 . 05 ) and 583 + / 525 for medium ( n = 6 ) ( P less than 0 . 01 ) . ^^^ Two RA SF were fractionated by Sephadex G 100 column chromatography ; low levels of CSF activity were detected in fractions corresponding to Mr of 70 100 kD , but the major CSF activity was found in the 20 24 kD fractions . ^^^ A polyclonal rabbit anti GM CSF antibody eliminated the stimulating activity from both rGM CSF and RA SF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The presence of muscular CP isoenzyme in the CSF of stroke patients was explained by increased blood brain barrier permeability , and blood admixture to SCF . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
A new growth factor ( synergistic factor , SF ) has recently been described , which , in combination with a macrophage CSF source , is able to stimulate the proliferation of primitive high proliferative potential macrophage progenitor cells ( HPP CFC ) in mouse bone marrow in culture . ^^^ It has been found that the addition of supraoptimal amounts of the crude CSF sources , extracts of pregnant mouse uterus and embryo , media conditioned by L cells or the mouse mammary tumor cell line ( EMT 6 ) , stimulated the proliferation of HPP CFC in the absence of any added SF . ^^^ This conclusion is supported by the results obtained from Sephacryl S 200 chromatography of these three CSF sources , which indicate that in all three cases fractions with apparent molecular weight ( MW ) greater than 68 , 000 contained the major portion of the CSF 1 activity , whereas fractions with MW less than 68 , 000 contained the SF like activity , together with minor amounts of CSF 1 activity . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
These properties of CSF 2 alpha and 2 beta are similar to those reported for interleukin 3 , hematopoietic cell growth factor , mast cell growth factor , and persisting cell growth factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
This G CSF copurifies with a mast cell growth factor but can be separated from M and GM CSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
After a control period during which both lateral ventricles were perfused with mock CSF with the same composition , the drug under study was added to one side ( experimental side ) while the other side was perfused further with the control mock SCF ( control side ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Clonal analysis of bone marrow and spleen cells suggested that CEE may contain the avian equivalents of stem cell factor , interleukin 3 , granulocyte macrophage CSF , granulocyte CSF , and macrophage CSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
SCF in combination with G CSF significantly augmented the proliferation of GM CFCs . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Although no significant effects were observed on granulocyte macrophage CSF ( GM CSF ) interleukin 3 or stem cell factor ( SCF ) induced proliferation , the combinations of G CSF and each of these cytokines were all inhibited . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Stem cell factor ( CSF ) , also called c kit ligand ( KL ) or mast cell growth factor ( MGF ) is a peptide growth factor / cytokine with broad activities , especially on hematopoiesis . ^^^ Recombinant human SCF has major clinical potential through its synergy with other factors , especially G CSF , to enhance mobilization of stem cells in peripheral blood . . ^^^ Stem cell factor ( CSF ) , also called c kit ligand ( KL ) or mast cell growth factor ( MGF ) is a peptide growth factor / cytokine with broad activities , especially on hematopoiesis . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Substance P ( SP ) , neurokinin A ( NKA ) , calcitonin gene related peptide ( CGRP ) and neuropeptide Y ( NPY ) like immunoreactivity ( LI ) was studied in rats ' cerebrospinal fluid ( CSF ) , plasma and synovial fluid ( SF ) from both knee joints at 2 and 24 h following an intraperitoneal administration of 0 . 05 ml human recombinant interleukin 1 alpha ( i . p . hrIL 1 alpha ) or saline . ^^^ There was a correlation in CGRP LI content between plasma and CSF following i . p . hrIL 1 alpha but not between plasma and SF or CSF and SF . ^^^ It can be concluded that ( 1 ) i . p . hrIL 1 alpha activates somatosensory afferents thereby increasing SP and CGRP LI content in CSF and plasma and NKA LI in CSF ; 2 ) i . p . hrIL 1 alpha induces a bilateral increase of CGRP LI in SF which is not mediated through systemic circulation and is possibly a part of the general host defensive reaction . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Herein , we show that the cytokines interleukin ( IL ) 3 , granulocyte macrophage CSF ( GM CSF ) , and KIT ligand ( KL ) are physiological activators of VLA 4 and VLA 5 expressed by MO7e , TF 1 , and normal bone marrow CD34+ progenitor cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
These results suggest that SF CSF estimations using commercially available assays could provide useful diagnostic clues for clinicians , but careful interpretation is warranted particularly in patients on long term NSAID treatment . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
OBJECTIVE To assess the influence of inflammatory synovial fluid ( SF ) on apoptosis of joint and blood neutrophils with particular reference to levels of colony stimulating factors ( CSF ) contained therein . ^^^ Separated neutrophils were cultured in vitro and exposed to : varying concentrations of SF in which CSF levels were measured , recombinant G CSF and GM CSF , and hyaluronic acid control solutions . ^^^ Numbers of apoptotic neutrophils and CSF levels were also measured in fresh SF samples . ^^^ CONCLUSION These data suggest that SF contains a factor or factors capable of directly or indirectly promoting neutrophil apoptosis and normally powerful enough to overcome the apoptosis inhibiting effects of cytokines such as GM CSF at concentrations usually found in inflammatory synovial fluids . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
While IL 1 or IL 6 had no proliferative effects as single growth factors , IL 1 , in combination with granulocyte macrophage CSF ( GM CSF ) , IL 3 , CSF 1 , and SCF , but not granulocyte CSF ( G CSF ) , enhanced the cloning frequency of Lin Sca 1+ progenitors three to five fold , whereas IL 6 increased the cloning frequency in response to all four CSFs and SCF two to seven fold . ^^^ Furthermore , the combined action of IL 1 and IL 6 resulted in additive or synergistic enhancement of CSF and SCF stimulated colony formation of Lin Sca 1+ high proliferative potential colony forming cells ( HPP CFCs ) . ^^^ Finally , IL 6 , but not IL 1 , enhanced the number of immature blast cells observed in CSF and SCF stimulated cultures . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Cerebrospinal fluid ( CSF ) and synovial fluid ( SF ) were obtained from patients during spinal puncture and athroscopy , respectively . ^^^ Only CSF and SF of patients with proven non pathological conditions were used in this study . ^^^ In non pathological conditions sIL 6R concentrations in CSF are 1 . 6 + / 0 . 4 ng / ml , and in SF 11 . 6 + / 3 . 3 ng / ml , while IL 6 concentrations are below detectable ranges in these fluids . ^^^ CONCLUSIONS : sIL 6R levels are detectable in serum , urine , CSF and SF during non pathological conditions . sIL 6R levels in serum outrange levels in CSF , urine and SF and large interindividual differences in baseline concentrations for sIL 6R exist . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The combination of bFGF or a FGF and G CSF , GM CSF , interleukin 3 , or stem cell factor ( SCF ) had a synergistic effect on blast colony formation in three patients . ^^^ In fact , bFGF was found to suppress the stimulation of blast colony formation due to GM CSF in one of 10 patients and that due to SCF in four of eight patients . aFGF suppressed the stimulation of blast colony formation due to GM CSF in two of 11 patients and that due to SCF in four of eight patients . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
This HFFF layer stimulated blood stem cells of GM CSF treated patients significantly more than a cocktail of exogenous growth factors with IL 1 , IL 6 , and stem cell factor ( SCF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Granulocyte macrophage colony forming cells ( GM CFC ) are bipotential progenitor cells that can proliferate and develop into macrophages in response to macrophage CSF or into neutrophils in response to stem cell factor or granulocyte CSF . ^^^ Calphostin C also blocked IL 4 mediated development of macrophages in stem cell factor and granulocyte CSF treated cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Third , we have tested the response to SCF of peripheral blood ( PB ) hemopoietic progenitors collected from patients receiving in vivo long term treatment with granulocyte CSF ( G CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Furthermore , soluble c kit ligand / rh stem cell factor ( rhSCF ) , in vitro in combination with rhIL 3 and rhGM CSF or PIXY 321 , increased the size but not the number of colonies derived from recovery phase BFU Meg and CFU Meg . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In contrast , colony and cluster formations from eight of nine c kitR cases was not stimulated at all by SCF alone nor SCF in combination with CSF nor with any CSF alone . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Enhanced gene expression for TNF alpha , macrophage CSF ( M CSF ) , and stem cell factor ( SCF ) was not detected . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
HSNC clones produced various cytokines , including IL 6 , IL 7 , IL 8 , IL 9 , granulocyte CSF ( G CSF ) , granulocyte macrophage CSF ( GM CSF ) , macrophage CSF ( CSF 1 ) , TGF beta 1 , and c kit ligand , but could not produce IL 1 alpha , IL 1 beta , IL 2 , IL 3 , IL 4 , TNF alpha , or TNF beta . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
These results indicate that CPA is a novel factor distinct from IL 1 , IL 3 , G , M , GM CSF , IL 6 and SCF ( c kit ligand ) . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The form of the RT / SF function : ( a ) replicated the inverse of the contrast sensitivity function ( CSF ) at near threshold contrast levels ; ( b ) behaved differently at higher contrasts , exhibiting two branches at contrast close to 1 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Bacterial endotoxin ( LPS ) modulation of CSF 1 , granulocyte macrophage ( GM ) CSF , G CSF , IL 1 , TNF , and Kit Ligand receptors on murine bone marrow cells ( BMC ) in vivo and in vitro was investigated . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Serum levels of stem cell factor ( SCF ) , interleukin ( IL ) 1 alpha , IL 6 , IL 3 , granulocyte macrophage CSF ( GM CSF ) and G CSF were analyzed before chemotherapy and during the myelosuppressive stage and correlations between cytokine levels and myelosuppression severity were examined . ^^^ Serum SCF levels did not fluctuate significantly during myelosuppression , and IL 1 , IL 3 and GM CSF were rarely detected in serum even after chemotherapy . ^^^ In the present study , the roles of IL 1 alpha , SCF , IL 3 and GM CSF chemotherapy induced myelosuppression were not clear . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Significantly , apoptosis of the cells occurred even in the presence of the survival factors granulocyte CSF and stem cell factor but was prevented by engagement of beta ( c ) with interleukin 3 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
To further address the role of IL 9 in the development of acute leukemia , we evaluated the proliferative response of three leukemic cell lines and 32 primary samples from acute myeloblastic leukemia ( AML ) patients to recombinant human ( rh ) IL 9 alone and combined with rh IL 3 , granulocyte macrophage CSF ( GM CSF ) , and stem cell factor ( [ SCF ] c kit ligand ) . ^^^ IL 9 stimulated 36 . 8 % of CFU L induced by phytohemagglutinin lymphocyte conditioned medium ( PHA LCM ) , and it was the most effective CSF for promoting leukemic cell growth among those tested in this study ( i . e . , SCF , IL 3 , and GM CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
PGE 2 enhanced the SF and IL 6 dependent development of mast cells from CBMC probably by blocking granulocyte macrophage CSF ( GM CSF ) secretion from accessory cells , because 1 ) PGE 2 , or anti GM CSF enhanced the mast cell development induced by SF and IL 6 from CBMC , but not from CD34+ cells ; 2 ) GM CSF inhibited the enhancing effect of IL 6 on the mast cell development from CD34+ cells ; and 3 ) PGE 2 inhibited GM CSF secretion from CBMC . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Human megakaryoblastic leukemia cell lines have common biological features : high expression of the megakaryocytic specific antigen ( CD 41 ) ; high expression of early myeloid antigens ( CD 34 , CD 33 and CD 13 ) ; constitutive expression of IL 6 and platelet derived growth factor ; a complex karyotype picture ; expression of c kit ( the stem cell factor receptor ) ; growth dependency or stimulation by IL 3 and / or GM CSF ; and in vivo tumorigenicity in mice associated with marked fibrosis . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We report here that the cytokine combination granulocyte macrophage CSF ( GM CSF ) plus TNF alpha and stem cell factor ( SCF ) commonly used for the in vitro generation of DC in serum / plasma supplemented medium is , in the absence of serum supplementation , very inefficient in inducing DC development . ^^^ Culture of CD34+ cells under serum free conditions with TGF beta 1 plus GM CSF , TNF alpha , and SCF strongly induces DC differentiation . ^^^ This culture condition is even more efficient than culturing CD34+ cells with GM CSF plus TNF alpha and SCF in the presence of cord blood plasma . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Using delayed addition of growth promoting cytokines , we demonstrate that FL potently promotes the in vitro survival of Lin Sca 1+ progenitors responsive to a potent myeloid growth factor combination ( FL , stem cell factor ( SCF ) , granulocyte CSF ( G CSF ) , and IL 1 alpha ) . ^^^ Whereas SCF was superior to FL in promoting the survival of FL , SCF , G CSF , and IL 1 alpha stimulated Lin Sca 1+ progenitors , FL was more efficient than SCF at promoting the survival of progenitors with a B cell potential , as measured by their ability to produce B220+ cells in response to delayed addition of FL , SCF , and IL 7 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Furthermore , MKE acted synergistically with granulocyte colony stimulating factor ( CSF ) , macrophage CSF , IL 6 and IL 11 on colony formation , but did not act with GM CSF , stem cell factor and Epo . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In quiescent HPCs the receptors for early acting [ flt 3 ligand ( FL ) , c kit ligand ( KL ) , bFGF , interleukin 6 ( IL 6 ) ] and multilineage [ IL 3 , granulocyte macrophage CSF ( GM CSF ) ] HGFs are expressed at significant levels but with different patterns , eg , kit and flt 3 are detected on a majority and minority of HPCs , respectively , whereas IL 3Rs and GM CSFRs are present on almost all HPCs . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
IGFBP 1 in serum , synovial fluid ( SF ) , cerebrospinal fluid ( CSF ) , and amniotic fluid ( AF ) were measured before and after treatment with alkaline phosphatase ( ALP ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
After two passages , total RNA was isolated from these confluent monolayers , fractionated on denaturing agarose gels , transferred to nylon membranes , and analyzed by Northern hybridization using complementary DNAs from epidermal growth factor ( EGF ) , stem cell factor , also known as Kit ligand , colony stimulating factor 1 ( CSF ) , leukemia inhibitory factor , and interleukin 6 ( IL 6 ) . ^^^ RESULT ( S ) : Human oviduct coculture cells express EGF ( kit ligand ) , CSF , leukemia inhibitory factor , and IL 6 . ^^^ Buffalo rat liver cells contain the messenger RNA transcripts for kit ligand and CSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Mice now exist lacking the granulopoietic factors G CSF , GM CSF , M CSF ( CSF 1 ) , SCF , several other factors influencing haemopoiesis ( including erythropoietin , interleukins 5 and 6 ) , combinations of these factors ( GM & M CSF ; G & GM CSF ; G & GM & M CSF ) and several CSF receptor components . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CD34+ cells were first expanded with the growth factors granulocyte macrophage CSF and stem cell factor to generate a population of intermediate progenitor cells with a relatively immature phenotype . ^^^ To induce specific differentiation to dendritic cells , the cultures were switched to serum free medium with the growth factors granulocyte macrophage CSF , stem cell factor , TNF alpha , and IL 4 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Low proliferative capacity was observed after 1 week in culture in the presence of stem cell factor ( SCF ) + Flt 3 ligand ( FL ) + G CSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Erythropoiesis was induced in HU 3 parental cells and five clones harvested from culture medium containing GM CSF by replacement of the growth promoting cytokine with stem cell factor ( SCF ) and erythropoietin ( Epo ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
However , mobilized CD34+ cells were significantly more responsive than their steady state BM counterparts to interleukin 3 ( IL 3 ) and stem cell factor ( SCF ) combined with G CSF or IL 3 in presence of erythropoietin ( Epo ) . ^^^ However , longer incubation with Ara C ( 16 to 18 hours ) , in the presence of SCF , IL 3 and G CSF , or IL 6 , showed that more than 60 % of LTC IC are actually cycling , with no difference being found with BM cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Urine , cerebrospinal fluid ( CSF ) , and SF specimens from 57 patients with Lyme borreliosis and from 58 controls were examined . ^^^ In clinical samples the diagnostic sensitivity of PCR was 85 % with SF samples , 79 % with urine samples , and 91 % with paired SF urine samples from patients with Lyme arthritis and was 79 % with CSF samples , 45 % with urine samples , and 87 % with paired CSF urine specimens from neuroborreliosis patients . ^^^ At least two different primer sets should be used , and whenever possible , urine and CSF or SF should be analyzed in parallel to achieve maximum sensitivity of the test . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CFU O was enriched in a nonadherent , low density , lineage marker negative ( Lin ) , Thy1 . 2 negative ( Thy1 . 2 ) , Sca 1 negative ( Sca 1 ) , and c kit positive ( c kit+ ) population , as were the progenitors that were responsive to macrophage colony stimulating factor ( CSF ; CFU M ) , granulocyte macrophage CSF ( CFU GM ) , and stem cell factor ( CFU SCF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Results show that ( 1 ) color CSF ' s are a low pass function of SF , whereas TE curves are a bandpass function of mask SF , and ( 2 ) a minimum of six SF tuned color mechanisms ( one low pass and five bandpass functions of SF , with peak SF ' s of 0 . 13 , 0 . 5 , 2 , 4 , and 8 cycles per degree and bandwidths of 3 . 9 , 4 . 4 , 2 . 9 , 2 . 1 , 1 . 1 , and 1 . 2 octaves ) , similar to oblique masking color mechanisms , are extracted by the multiple mechanisms model . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
These studies demonstrate for the first time that ( 1 ) mouse oval cells respond to a wide variety of GDFs including various members of the interleukin , chemokine , stem cell factor , EGF , FGF , PDGF , TGF beta , VEGF , insulin , CSF , TNF , HGF , and IFN growth and differentiation factor families in addition to multiple comitogens and ( 2 ) during tumorigenic conversion mouse oval cells undergo alterations which result in both alterations in GDF responsiveness and the autocrine / paracrine production of multiple GDFs . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
METHODS : a ) CFU GM , CFU GEMM and BFU E cultures in the presence of CSF , BM serum or HUCB serum ; b ) 35 day LTC in liquid media whether in presence of IL 3 + GM CSF + SCF or autologous serum ( AS ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Furthermore , TPO increased the colony stimulatory effects of G CSF , GM CSF , IL 3 , and stem cell factor ( SCF ) on MDS marrow cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The further observation that SF EC cultures elaborated comparable amounts of granulocyte macrophage ( GM ) CSF as FCS cultured EC , but were relatively impaired in their IL 1alpha and TNF alpha production , supports the role of GM CSF in LC maturation and , less so , in LC survival . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Bone marrow cells cultured for high proliferative potential CFCs in the presence of interleukin ( IL ) 1 , IL 3 , macrophage CSF , and stem cell factor ( SCF ) , or cultured for erythroid burst forming units in the presence of IL 3 , SCF , and erythropoietin , also exhibited enhanced growth in the presence of PGG glucan . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
However , mobilized CD34+ cells were significantly more responsive than their steady state BM counterparts to interleukin 3 ( IL 3 ) and stem cell factor ( SCF ) combined with G CSF or IL 3 in the presence of erythropoietin ( Epo ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We examined the effect of recombinant human interleukin ( IL ) 11 alone or in combination with various colony stimulating factors ( CSFs ) , including IL 3 , granulocyte / macrophage ( GM ) CSF , granulocyte ( G ) CSF , stem cell factor ( SCF ) , flt 3 ligand ( FL ) , and thrombopoietin ( TPO ) , on colony formation by leukemic progenitor cells ( L CFU ) obtained from 33 patients with acute myelogenous leukemia ( AML ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Infection of MYA 1 cells , a CD4+ T lymphoblastoid cell line , with FeLV A activated expression of macrophage inflammatory protein 1alpha ( MIP 1alpha ) , increased transcript levels of GM CSF ( 8 fold ) , macrophage CSF ( M CSF ) ( 16 fold ) and stem cell factor ( SCF ) ( 250 fold ) , and decreased ( 4 fold ) expression of IL 10 and tumor necrosis factor alpha ( TNF alpha ) . ^^^ Productive infection with four different FIV molecular clones caused progressive MYA 1 cell death ; however , the mRNA expression profiles were unchanged except for 2 to 4 fold increases in M CSF and 16 to 500 fold increases in SCF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The addition of Stem Cell Factor ( SCF ) or IL 6 to the standard combination of flt 3L+ / GM CSF produces a large increase in the proliferation of GM and DC progenitors ( 28 times and 11 times respectively ) in the first step of the culture . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
METHODS : Cardiac samples from 15 autopsied subjects ( HD , n=6 ; control , n=9 ) were sequentially extracted with 0 . 9 % NaCl and collagenase to obtain two fractions [ soluble fraction ( SF ) and collagenase soluble fraction ( CSF ) ] . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Soluble dlk IgG Fc chimeric protein completely inhibited the colony formation of lineage marker negative ( Lin ) bone marrow cells by GM CSF , G CSF , or macrophage CSF ( M CSF ) in the presence of stem cell factor ( SCF ) . ^^^ Furthermore , dlk failed to inhibit the colony formation of Hes 1 null fetal liver cells by M CSF in the presence of SCF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The effect of granulocyte colony stimulating factor ( G CSF ) induced mobilization of peripheral blood progenitor cells ( PBPC ) on the endogenous serum levels of cytokines stem cell factor ( SCF ) and flt 3 ligand ( flt 3 L ) was studied in 18 healthy subjects undergoing allogeneic PBPC donation . ^^^ Endogenous serum flt 3 L and SCF were determined prior to G CSF administration , on the day of leukapheresis , and followed up until day +100 after cessation of G CSF administration . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Exposure of HUVECs to ethanol ( 0 . 01 % 1 % ) dose dependently inhibited ( by 12 % 27 % ) the release of stem cell factor , granulocyte macrophage and granulocyte colony stimulating factors ( CSFs ) , or interleukin ( IL ) 8 , but not of macrophage CSF triggered by lipopolysaccharide ( LPS ) or IL 1 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Granulocyte macrophage CSF , stem cell factor , interleukin 3 , and vascular endothelial growth factor could not similarly replace the need for M CSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Meis1a suppresses differentiation by G CSF and promotes proliferation by SCF : potential mechanisms of cooperativity with Hoxa 9 in myeloid leukemia . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Apart from granulocyte macrophage CSF ( GM CSF ) , there have as yet been no reports of lithium salts inducing early activating factors for the megakaryocytic lineage , such as IL 3 , IL 11 , stem cell factor and flt 3 ligand , or maturation factors , such as thrombopoietin ( TPO ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Results show the following : ( 1 ) The color CSF is a low pass function of SF with average half height SF of 0 . 7 cpd and cutoff SF of 14 cpd with the use of a color detection criterion . ( 2 ) TvSF curves are broadly bandpass and fall into five groups , peaking at approximately 0 . 13 , 0 . 5 , 2 , 4 , and 8 cpd . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Sera , synovial ( SF ) and cerebro spinal fluids ( CSF ) were obtained from 90 patients with clinical symptoms of Lyme borreliosis . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We show here that exogenous Emi 1 is unstable in CSF arrested Xenopus eggs and is destroyed by the SCF ( betaTrCP ) ubiquitin ligase , suggesting that endogenous Emi 1 , an apparent 44 kDa protein , requires a stabilizing factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
ES CM contained interleukin ( IL ) 1alpha , IL 10 , IL 11 , macrophage colony stimulating factor ( CSF ) , oncostatin M , stem cell factor , vascular endothelial growth factor , as well as a number of chemokines and other proteins , some of which are known to enhance survival / anti apoptosis of progenitors . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The APC / C inhibitor Emi 2 or XErp 1 has recently been characterized as a pivotal CSF component , required to maintain metaphase 2 arrest and rapidly destroyed in response to Ca ( 2+ ) signaling through phosphorylation by Plx 1 and ubiquitination by the SCF ( betaTrCP ) complex . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The hitherto known growth factors are divided into four basic groups : 1 . haematopoietic growth factors proper [ multi CSF ( IL 3 ) , GM CSF , G CSF , M CSF , erythropoietin , lymphopoietin ( IL 7 ) and megakaryopoietin ( IL 11 ) ] , 2 . cytokines ( IL 1 to IL 11 , TFN ) . , 3 . other growth factors ( PDGF , FGF , MGF ) and 4 . so called negative regulators of haematopoiesis ( IFN , MIP , TGF beta and IL 10 ) , some of which support the differentiation of stem cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The authors present an account of haematopoietic growth factors which include the stem cell factor ( SCF ) , factors stimulating granulocytomacrophage colonies ( GM CSF ) , granulocyte ( G CSF ) , monocytomacrophage ( M CSF ) factors , erythropoietin ( EPO ) and also interleukin ( IL ) 1 to 11 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Human mast cell growth factor ( MGF , a c kit ligand ) and colony stimulating factors ( Epo , GM CSF , G CSF , IL 3 ) were assessed in the absence or presence of serum for stimulation in semi solid medium of single CD 34 , CD 34 HLA DR+ , or CD 34 HLA DR+CD33 cells sorted per microtiter well . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The present studies analyzing stromal cytokine messenger RNA ( mRNA ) production by standard Northern blot analysis show constitutive production of mRNAs for CSF 1 , GM CSF , granulocyte CSF ( G CSF ) , c kit ligand ( KL ) , and interleukin 6 ( IL 6 ) , but not IL 3 , IL 4 , or IL 5 by 3 week irradiated or nonirradiated murine Dexter stromal cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
IL 3 and stem cell factor / mast cell growth factor ( SCF ) showed only a marginal effect , and granulocyte macrophage and macrophage CSFs ( GM CSF and M CSF , respectively ) were inactive . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In the present study , we have addressed the mechanism of synergistic growth activation , at the cellular level , by SF and GM CSF using the blast cells of acute myeloblastic leukemia ( AML blasts ) . ^^^ Analysis of cultures performed in the presence of SF and GM CSF at different cell concentrations , ranging from 1 , 000 to 20 , 000 cells , suggested a single limiting element , i . e . , the blast clonogenic cell , while 2 or more limiting elements were found in cultures stimulated with GM CSF alone , suggesting interacting cell populations . ^^^ In all samples tested , SF appears to increase the responsiveness of AML blasts to GM CSF , as demonstrated by a 3 fold decrease of GM CSF half efficient concentration on addition of SF to the cultures . ^^^ Exposure of AML blasts to SF did not affect GM CSF receptor expression , suggesting that this increase in GM CSF responsiveness is likely to occur at the postreceptor level . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
AIC2A and AIC2B transcription was transiently induced in these cells in response to a variety of hematopoietic growth factors , including erythropoietin ( EPO ) , monocyte CSF , IL 3 , GM CSF , and stem cell factor ( SCF or kit ligand ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Recombinant human stem cell factor synergises with GM CSF , G CSF , IL 3 and epo to stimulate human progenitor cells of the myeloid and erythroid lineages . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The line grows slowly without stimulation but responds to interleukin 3 ( IL 3 ) , GM CSF and mast cell growth factor ( MGF ) , a ligand for the receptor encoded by the c kit oncogene . ^^^ When OCI / AML 4 cells are exposed to MGF with IL 3 or GM CSF , additive or synergistic effects are seen . ^^^ The cells are more ara C sensitive in MGF or G CSF than in IL 3 or GM CSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The TF 1 cell line responds to rhu IL 3 , rhu GM CSF and to a lesser extent to rhu Erythropoietin ( EPO ) and mast cell growth factor ( MGF ) , but not to rhu G CSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Type 1 T helper cells ( TH 1 ) produced IL 2 , interferon gamma , GM CSF , and IL 3 in response to antigen + presenting cells or to Con A , whereas type 2 helper T cells ( TH 2 ) produced IL 3 , BSF 1 , and two other activities unique to the TH 2 subset , a mast cell growth factor distinct from IL 3 and a T cell growth factor distinct from IL 2 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In order to study its mechanism of action and to explore the molecular basis for its synergistic activity we have examined the proteins that become tyrosine phosphorylated in response to SF , IL 3 , and GM CSF . ^^^ We report herein that SF , but not IL 3 or GM CSF , dramatically stimulates the tyrosine phosphorylation of the product of the recently discovered proto oncogene , vav , in two SF responsive human cell lines , M07E and TF 1 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We have probed the pathways activated by SF and GM CSF in suppression of active cell death ( apoptosis ) using two classes of inhibitors : Tyrphostins that are specific inhibitors of protein tyrosine kinase , and amiloride derivatives ( 5 ( N , N ethyl n isopropyl ) amiloride and 5 ( N , N hexamethylene ) amiloride ) that have been designed as specific inhibitors of the Na+ / H+ antiporter . ^^^ Both SF dependent and GM CSF dependent pathways are sensitive to inhibition by Tyrphostins with , nonetheless , a quantitative difference . ^^^ In contrast to the discrepancy in Tyrphostin dose response curves , titration curves for 5 ( N ethyl n isopropyl ) amiloride and 5 ( N , N hexamethylene ) amiloride are comparable in SF or GM CSF stimulated cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Finally , involvement of an autocrine loop of activation induced by MS 5 was excluded : RT PCR analysis did not detect increased transcripts for GM CSF , IL 3 , IL 6 , SCF , or Epo in UT 7 cells cocultured for 2 to 6 days with MS 5 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In this study , we have attempted to relate the murine HPP CFC , stimulated by various combinations of growth factors ( GFs ) CSF 1 , GM CSF , IL 3 , IL 6 , IL 1 , stem cell factor ( SCF ) , and transforming growth factor beta ( TGF beta ) and by CSF 1 , GM CSF , and IL 3 on their own , to these known progenitors . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The human factor dependent cell line MO 7 has been used as a model to study the interaction of SF with other growth factors such as GM CSF , because both factors support the proliferation of this cell line and are synergistic in combination . ^^^ In an attempt to further explore the biological and biochemical mechanisms of the synergy between SF and GM CSF , we examined the effects of these growth factors on the regulation of nuclear proto oncogenes , cell cycle control genes , and G 1 > S transition of MO 7 cells . ^^^ Individually , GM CSF was a much more potent growth factor for MO 7 cells than SF , particularly under serum free conditions . ^^^ Only GM CSF , but not SF , was able to stimulate G 1 > S transition of MO 7 cells after factor deprivation for 24 h . ^^^ Northern blot analyses showed also differential effects of GM CSF and SF on the expression of some nuclear proto oncogenes and G 1 cyclins . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Synergistic activities were seen when FL was combined with G CSF , GM CSF , IL 3 , or stem cell factor ( SCF ) . ^^^ In the presence of suboptimal and optimal concentrations of G CSF , GM CSF , IL 3 , SCF , or a combination of all factors , FL strongly enhanced the number of leukemic colonies ( up to 18 fold ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , the ligand of the c kit receptor , is a potent enhancing cytokine for haematopoietic cells in the presence of IL 3 , GM CSF and erythropoietin ( Epo ) . ^^^ In the clonogenic assays of 63 MDS patients , the addition of rh SCF + GM CSF and / or IL 3 induced a significant increase ( p < 0 . 001 ) in the number and size of CFU GM . ^^^ Stem cell factor ( SCF ) , the ligand of the c kit receptor , is a potent enhancing cytokine for haematopoietic cells in the presence of IL 3 , GM CSF and erythropoietin ( Epo ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
To determine if the c kit ligand mast cell growth factor ( MGF ) can improve progenitor growth in MDS , we evaluated in vitro responsiveness of bone marrow progenitors from 25 patients to MGF and / or GM CSF , interleukin 3 ( IL 3 ) and PIXY 321 , and examined the relationship between progenitor response and cellular expression of the c kit receptor . ^^^ Among 17 patients with absent growth in lymphocyte conditioned media , MGF stimulated CFU GEMM recovery in 59 % , compared to 23 % with PIXY 321 , 12 % with IL 3 and 8 % with GM CSF . ^^^ MGF and / or IL 3 were comparatively weak stimulants of CFU GM formation , whereas GM CSF and PIXY in combination with MGF increased colony number 2 to 15 fold in 60 and 70 % of patients , respectively , while preserving maturation competence as evidenced by colony composition and increased colony / cluster ratio . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We demonstrated that MDS marrow hematopoietic progenitor cells reactive to GM CSF or EPO were additionally stimulated with early acting hematopoietic growth factors including IL 6 , IL 3 and SCF in a fashion similar to those of normal individuals . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
However , if combined with GM CSF , which per se downregulates the SCF R on these cells as well , TNF alpha antagonized the effect of GM CSF and slightly increased the density of SCF R . ^^^ Yet the cells incubated for 24 hours in medium without cytokines invariably expressed a higher level of SCF R than the cells incubated in the presence of TNF alpha and GM CSF , either alone or in combination . ^^^ In contrast , no such morphological changes could be observed in the presence of GM CSF or SCF . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
These include kit ligand ( KL ) , interleukins ( IL ) 1 , 3 and 6 and granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In addition , rapamycin blocks the proliferative response of cell lines to a variety of hematopoietic growth factors , including interleukin 3 ( IL 3 ) , interleukin 6 ( IL 6 ) , granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and kit ligand ( KL ) , suggesting that it should be a strong inhibitor of hematopoiesis . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Because of the importance of cytokines in the regulation of allergic inflammation , we investigated the effects of SCF , IL 3 , IL 5 and GM CSF on immunological histamine release from sensitized human lung fragments as well as human leukocytes . ^^^ Modulation of immunological histamine release from human lung fragments by stem cell factor , IL 3 , IL 5 and GM CSF : comparison with human leukocytes . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Single factors such as IL 3 , IL 6 , GM CSF , and stem cell factor did not optimally stimulate growth of cord blood CFU GM . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Using this system , CD34+ cells isolated from bone marrow were cultured in the presence of GM CSF , G CSF , erythropoietin , stem cell factor , IL 1 , IL 3 , and IL 6 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Taken together , these data demonstrate that IL 3 is a major early mast cell growth factor , that mast cells become more dependent on SCF with time , and that the effects of IL 3 and SCF are upregulated ( IL 4 ) or downregulated ( M CSF , GM CSF , IFN gamma ) by both growth factors and proinflammatory cytokines . . ^^^ Mouse bone marrow ( BM ) was cultured in the presence of recombinant mouse ( rm ) interleukin 3 ( IL 3 ) , rmIL 4 , rmIL 5 , rmIL 7 , purified mouse ( m ) IL 9 , rmIL 10 , recombinant human ( rh ) macrophage colony stimulating factors ( M CSF ) , rm granulocyte macrophage colony stimulating factors ( GM CSF ) rm stem cell factor ( SCF ) , rh interferon alpha ( IFN alpha ) , rmIFN gamma , and mNGF to determine which cytokine would give rise to mast cells in murine BM cultures . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
A study of gene expression of GM CSF , IL 3 , c kit ligand , and IL 1 by bone marrow stromal cells of mice who were treated with an LD 50 cytosine arabinoside ( Ara C ) dose was carried out . ^^^ We conclude that GM CSF , c kit ligand and IL 1 have important roles in sustaining marrow regeneration following extensive damage . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In this preliminary experience we found that , in vitro , rhEPO potentiates the effect of rhG CSF on HP growth and differentiation toward the granulocyte macrophage lineage . rhEPO plus rhG CSF produced in vitro a proliferative stimulus of HP which represents 26 % of the maximum stimulation obtained using IL 1 , IL 3 , IL 6 , G CSF , GM CSF and stem cell factor in combination . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The expression of an exogenous c myc proto oncogene or a 12H ras oncogene lowered the requirement of FDC P 1 cells for IL 3 or GM CSF , in a similar manner to M CSF or SCF addition . ^^^ These results strongly support a role for ras and myc in the synergistic action of M CSF and , by implication , of SCF , which implies that these signalling intermediates are rate limiting for the action of IL 3 and GM CSF and possibly other haemopoietic growth factors . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In vitro colony assay revealed that the combination of PSK and any of GM CSF , IL 3 or stem cell factor ( SCF ) showed a greater increase in colony numbers than when these materials were administered individually , although G CSF did not show a synergistic effect with PSK . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Inclusion of SCF , together with IL 3 , GM CSF or both cytokines stimulated growth of these cells over 14 day period . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We show that treatment of UT 7 cells with EPO , GM CSF or stem cell factor ( SCF ) rapidly induces phosphorylation of p95B raf as indicated by a shift of electrophoretic mobility . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
GM CSF or mast cell growth factor ( MGF ) , whereas it was undetectable in growth factor deprived cells . ^^^ By means of the M 07e bioassay , which allows the detection of GM CSF , IL 3 and MGF activities , and neutralizing antibodies to each of these factors , GM CSF activity was detected in the cell free extract of both IL 3 and MGF sustained cells and of cells deprived for 24 h . ^^^ This finding demonstrates that M 07e cells produce and store biologically active GM CSF in response to both IL 3 and MGF . ^^^ In contrast , analysis of the growth stimulatory activity present in the culture supernatants revealed that MGF , unlike IL 3 , is able to induce the secretion of consistent amounts of GM CSF . ^^^ Taken together , our results suggest that , in M 07e cells , GM CSF gene transcription and GM CSF production are mediated , unlike its secretion , by mechanisms shared by IL 3 and MGF . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In the presence of optimal concentrations of recombinant interleukin 3 ( IL 3 ) , stem cell factor ( SCF ) , granulocyte / macrophage colony stimulating factor ( GM CSF ) , and erythropoietin ( Epo ) , low and high doses of RA induced striking differential effects on CD34+ cell proliferation in liquid cultures and colony formation in semisolid assays . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , a c kit ligand , has a preferential effect on the proliferation of several classes of immature hematopoietic progenitor cells in combination with GM CSF or IL 3 . ^^^ To analyze the costimulatory role of SCF in leukemic growth , we investigated the effect of SCF in the presence of GM CSF and / or IL 3 on isolated CD 34 positive ( CD34+ ) leukemic blasts from 15 patients with acute myelogenous leukemia ( AML ) . ^^^ When the proliferation of CD34+ AML blasts in the presence of GM CSF and / or IL 3 were evaluated in vitro for the effects of SCF , two patterns emerged . ^^^ In one pattern , CD34+ AML blasts responded with a significant increase in DNA synthesis and / or colony formation when SCF was used with GM CSF and / or IL 3 relative to the growth with SCF alone ; This result is consistent with those CD34+ bone marrow cells from normal donors . ^^^ In the other pattern , nine of the patients ( 60 % ) had CD34+ leukemic cells whose growth with SCF plus either GM CSF , IL 3 , or GM CSF+IL 3 , was not significantly different from the growth noted in the presence of SCF alone . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
When either IL 4 or MGF was added to the combination of IL 3 , GM CSF , G CSF , and IL 6 , a statistically significant increase in the colony number was observed . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Culture of isolated CD 34 cells in liquid culture with interleukin 3 , stem cell factor , G CSF , and granulocyte macrophage GM CSF showed over a 100 fold expansion in cell numbers after 25 days , with the peak expansion of colony forming cells occurring between days 11 and 16 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
This defect is characterized by suppression of a subpopulation of IL 3 responsive cells and ablation of stem cell factor synergy with GM CSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The present study is to evaluate , in addition to TNF alpha and G CSF , whether serum stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) and granulocyte macrophage CSF ( GM CSF ) levels to see if they were also elevated in patients with acute bacterial infection . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Recombinant cytokines including GM CSF , G CSF , IL 1 beta , IL 6 , stem cell factor , LIF , erythropoietin , and IL 3 had no effect on P gp expression whereas TNF alpha induced dose and time dependent P gp and mdr 1 gene overexpression . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
A large fraction ( > 30 % ) of single cell / well sorted CD34bright / RhLA DRdull cells formed multilineage colonies after 2 to 4 weeks of stimulation with IL 3 , GM CSF , Kit ligand , and IL 6 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
TGF beta 1 at 80 pM exhibited differential effects on the normal hematopoietic progenitors when cells were stimulated with different growth factors , such as G CSF , GM CSF , interleukin 3 ( IL 3 ) , or stem cell factor ( SCF ) . ^^^ The suppressive effect by TGF beta 1 was increased for growth with GM CSF , IL 3 , and SCF , and growth with G CSF was unaffected in hematologic malignancies , TGF beta 1 suppression for growth with G CSF was increased for essential thrombocythemia ( ET ) and polycythemia vera ; chronic myelogenous leukemia ( CML ) in chronic phase ; CML in accelerated phase ; CML in myeloid crisis ; myelodysplastic syndrome ( MDS ) in refractory anemia ; MDS in refractory anemia with an excess of blasts ; and acute myeloblastic leukemia ( AML ) . ^^^ The mean ED50s for the growth of leukemic blast progenitors were 1 . 6 , 1 . 2 , 0 . 7 , and 0 . 2 pM in the presence of G CSF , GM CSF , IL 3 , and SCF , respectively , c myc and c myb antisense oligonucleotides significantly suppressed the growth of leukemic blast progenitors , but not that of clonogenic cells from normal donors and patients with ET . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Leukocytes from 12 patients with documented anaphylactic sensitivity to wasp venom were preincubated in the presence or absence of IL 3 , granulocyte / macrophage colony stimulating factor ( GM CSF ) , IL 5 , IL 8 , or stem cell factor ( SCF ) . ^^^ Preincubation of leukocytes with IL 3 , GM CSF , or IL 5 ( 0 . 02 2 ng / ml ) , but not with IL 8 and SCF , caused a dose dependent enhancement of antigen induced basophilic histamine release in all subjects tested . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
While interleukin ( IL ) 3 and IL 5 ( 20 ng / ml ) were unable to modulate immunologic HR , GM CSF ( 20 ng / ml ) produced significant potentiation ( P < 0 . 05 ) , which was , however , smaller than that observed with SCF . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We describe a subclass of agar based high proliferative potential colony forming cells ( HPP CFC ) derived from populations enriched for early hematopoietic progenitors which , when stimulated with the combination of CSF 1 , G CSF , GM CSF , IL 1 alpha , IL 3 and SCF , produces colonies with trilineage potential for macrophages , granulocytes , and megakaryocytes . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
FSDC stimulated poorly allogeneic or syngeneic T cells in the primary mixed leukocyte reaction , and markedly increased this function after treatment with GM CSF , GM CSF and interleukin ( IL ) 4 or interferon gamma ( IFN gamma ) ; in contrast , stem cell factor , IL 1 alpha and tumor necrosis factor alpha had no effect . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Neither SCF , IL 3 , GM CSF nor TNF alpha were found in conditioned media of long term bone marrow cultures ( LTBMC ) of normal or MAIDS mice . ^^^ Addition of soluble GM CSF and SCF only transiently increased the production of CFUs ( BFUE and CFU G / M ) in MAIDS LTBMC . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
A hierarchy appeared clearly regarding sensitivity to the drug in the following order of increasing resistance : PCM CFU GM ( grown in presence of placenta conditioned medium ) , 4R CFU GM ( grown in presence of GM CSF + IL 3 + G CSF + EPO ) , 5R CFU GM ( grown in presence GM CSF + IL 3 + G CSF + EPO + SCF ) and LTC IC with respective lethal dose 95 ( LD 95 ) levels of 40 micrograms , 55 micrograms , 90 micrograms and 140 micrograms / 10 ( 7 ) MNC ( P = 0 . 05 to P = 0 . 018 ) . ^^^ Even the primitive stem cells treated with very high doses of mafosfamide ( 2 to 4 times the usually recommended dose ) responded to a combination of SCF + GM CSF + G CSF + IL 3 in liquid marrow culture , suggesting that they were functionally spared by the treatment . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The response of B cell precursors to the cytokine interleukin 7 is variably affected while that of several other cytokines ( stem cell factor , interleukin 3 , GM CSF , G CSF , and erythropoietin ) is normal in c abl mutants . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
It is particular mechanism that we have investigated , showing that molecules such as GM CSF , G CSF , IL 6 , IL 8 and SCF can be produced by airways epithelial cells and fibroblasts in quantities sufficient to induce hemopoietic events , either systemically or locally . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We examined the morphological and phenotypic characteristics and cell cycle status of the subpopulations and determined the responsiveness of granulocyte monocyte progenitors ( colony forming units / granulocyte macrophage ) derived from each subpopulation to mast cell growth factor in combination with granulocyte ( G CSF ) or granulocyte macrophage ( GM CSF ) colony stimulating factors alone and in combination . ^^^ Mast cell growth factor addition to cultures stimulated by G CSF , GM CSF , or G CSF plus GM CSF , exerted the greatest synergistic effect ( increased colony number and size ) in the normal small and intermediate lin blast cultures , but mast cell growth factor had considerably less effect , or no effect , in cultures of comparable CML subpopulations , indicating that CML lin progenitors had a somewhat lower requirement for multiple growth factors . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
SCF at a determined optimum concentration of 100 ng / mL added to the combination of IL 3 , GM CSF , and G CSF enhanced colony growth and size relative to proliferation in response to the latter three factors alone ( from 78 to 188 total colonies / 10 ( 4 ) PBHP plated and from 10 to 93 large [ > 200 cells ] colonies / 10 ( 4 ) PBHP plated ) . ^^^ Furthermore , addition of bFGF and / or IL 6 to the combination of optimum concentrations of SCF , IL 3 , GM CSF , and G CSF further enhanced colony number and size in a dose dependent fashion . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The effects of interleukin 1 beta ( IL 1 ) , IL 3 , IL 6 , granulocyte macrophage CSF ( GM CSF ) , G CSF , macrophage CSF ( M CSF ) , stem cell factor ( SCF ) , erythropoietin ( EPO ) , leukemia inhibitory factor ( LIF ) , and interferon gamma , as well as 36 combinations of these factors were tested . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
SCF plays an important role in the GM CSF or IL 3 dependent production of neutrophils and macrophages . ^^^ In vitro colony assay also suggests an increase in sensitivity of GM progenitors to cytokines ( GM CSF , IL 3 , G CSF and / or SCF ) in a patient with juvenile chronic myelogenous leukemia . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
SCF stimulated leukemic blast progenitors in a manner almost comparable to or more prominently than that of other CSFs , namely , IL 3 , GM CSF , and G CSF , in 9 patients . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Human stem cell factor ( c kit ligand ) induces an autocrine loop of growth in a GM CSF dependent megakaryocytic leukemia cell line . ^^^ In fact , in short term proliferation assays the stimulatory activity of SCF is blocked about 75 % by a GM CSF specific serum . ^^^ In addition , we could detect GM CSF expression by SCF stimulated M07e cells , both at the protein and mRNA levels . ^^^ Overall , these data show that the ability of SCF to support the growth of this megakaryocytic cell line is mediated mostly by the induction of an autocrine loop of activation involving GM CSF production . ^^^ The finding that SCF can stimulate GM CSF secretion also in an IL 2 dependent T lymphoblastic leukemia cell line indicates that SCF can act on cells of both myeloid and lymphoid lineages , and that the ability to induce cytokines in target cells represents an important aspect of its mechanism of action . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In vivo daily administration of SCF plus GM CSF results in a synergistic increase in marrow neutrophils , but not the striking synergistic increase in circulating neutrophils that is observed after SCF plus G CSF . ^^^ Colony forming assays reveal a synergistic increase in CFU GMs in the marrow , but not in peripheral blood , after coincubation with SCF plus GM CSF as opposed to GM CSF alone , demonstrating anatomic compartmentalization between a more primitive marrow CFU GM subset and a more mature peripheral blood CFU GM subset . . ^^^ Hematologic effects of stem cell factor alone and in combination with G CSF and GM CSF in vivo and in vitro in rodents . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Overall , our data indicate that STAT 5 is widely expressed in hematopoietic cells and activated by a number of cytokines , including TPO , GM CSF and EPO , but not by IFN gamma or SCF . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Although neither stem cell factor , nor GM CSF alone can significantly induce the proliferation of stem cells , the combination induces the proliferation of these cells . ^^^ Committed progenitor cells such as granulocyte macrophage colony forming cells , however , are stimulated to proliferate by GM CSF alone , while stem cell factor in combination with GM CSF results in only a slight additive effect . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Culture supernatants from blasts cultured with or without TPA showed the production of large amounts of IL 8 , IL 6 , TNF alpha , MIP 1 alpha , IL 10 and interferon gamma and modest amounts of IL 1 alpha , GM CSF and stem cell factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Particularly the identification of new surface membrane molecules such as CD 40 ligand on basophils or c kit on mast cells , and of new triggering agents and modulators of mediator release such as IL 3 , IL 5 , GM CSF and nerve growth factor ( for basophils ) or c kit ligand ( for mast cells ) allows a better understanding of the regulation of these cell types . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The comparative study of the cytokine gene expression in MS 5 and in the nonsupportive cell lines included Northern blot and reverse transcriptase polymerase chain reaction analysis of messenger RNA for interleukin 1 , 3 , 6 , granulo macrophage colony stimulating factor ( GM CSF ) , granulocyte CSF , macrophage CSF , stem cell factor , transforming growth factor beta , tumor necrosis factor alpha , macrophage inflammatory protein 1 alpha , and leukemia inhibitory factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The growth factor combination in the perfusion medium consisted of interleukin 3 ( IL 3 ) , stem cell factor ( SCF ) , GM CSF and erythropoietin ( Epo ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Expansion of marrow in all cases was accomplished through the use of added cytokines such as IL 3 , GM CSF , and c kit ligand . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We compared the ability of SF and GR cells to sustain in vitro proliferation of the FDC P 1 myeloid cell line , dependent upon IL 3 or GM CSF . 3 . ^^^ The same activity could be restored to NaCl treated GR cells , but not to SF , by incubation with recombinant murine GM CSF . 4 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
After vector exposure , optimal gene transfer was noted when CML mononuclear cell cultures contained stem cell factor , IL 3 , GM CSF and erythropoietin . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
GF dependent samples had significant proliferative responses to steel factor ( SF ) , GM CSF and IL 3 ; mean Ki67+ cell number increased by 4 . 1 , 3 . 3 , and 5 . 2 fold , respectively . ^^^ SF was additive or synergistic with GM CSF or IL 3 among GF dependent cases ; however , combinations were no more effective than single cytokines among the GF independent group . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Low doses of IL 2 ( 0 . 1 1000 U / ml ) were studied without or in combination with recombinant IL 3 , GM CSF and erythropoietin , in SF long term marrow culture ( LTMC ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The remainder ( interleukins 1 to 11 , G CSF , GM CSF , LIF , SCF , erythropoetin , IFN gamma , RANTES , MIP 1 alpha , FGF , EGF , PDGF , dexamethasone , forskolin , retinoic acid and cyclosporin A ) failed to alter expression . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Neither stromal cell co culture nor exposure of factor dependent and factor independent cell lines to GM CSF , IL 3 , SCF , or combinations thereof significantly changed the percentage of cells expressing Ki 67 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
After pre exposition to rhIL 3 , IL 1 beta , rIL 6 , GM CSF and stem cell factor ( SCF ) and cloning with SCF the number of hematopoietic progenitors increased 14 18 fold compared to basal values . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Neutralizing antibodies to IL 3 , GM CSF , and mast cell growth factor inhibited proliferation of the factor responsive clones by 60 % to 90 % . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
However , combinations of cytokines present in conditioned media from the T cell lines MO or C5MJ , or defined multiple cytokine combinations containing IL 1 , IL 3 , GM CSF , kit ligand , IL 6 , and IL 9 , restored myelopoiesis in vitro . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In two LTCs initiated in the blastic stage , we demonstrated the selective effect of three culture media ( serum dependent , serum free ( SF ) supplemented or not with IL 3 and GM CSF ) on the proliferative potential of normal hematopoietic ( CFU GM / BFU E ) and leukemic progenitors ( CFU ALL ) . ^^^ The addition of IL 3 and GM CSF to the SF medium enhanced the growth of CFU GM / BFU E and shortened the purging period . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Cross reactivity between GFRs and heterologous GFs ( including IL 6 , IL 3 , GM CSF , Ep , and the kit ligand [ KL ] ) was explored by competition experiments on purified progenitors with radiolabeled and excess cold HGFs at +4 degrees C . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The expression of interleukin ( IL ) 7 , stem cell factor ( SCF ) , IL 1 alpha and granulocyte monocyte colony stimulating factor ( GM CSF ) mRNA was mapped to individual stromal cell types , while the expression of IL 1 alpha and GM CSF , along with interferon ( IFN ) gamma and IL 4 was detected in the lymphoid compartment of fetal day ( Fd ) 14 thymus . ^^^ Purified major histocompatibility complex class II+ cortical epithelial cells strongly expressed IL 7 and SCF , but only limited expression of IL 1 alpha and GM CSF could be detected . ^^^ Fetal mesenchyme , on the other hand , expressed SCF , IL 1 alpha and GM CSF but not IL 7 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
This effect is further increased by addition of other cytokines such as GM CSF , IL 3 , IL 4 , Stem cell factor ( SCF ) , while Tumor Necrosis Factor ( TNF ) and Transforming Growth Factor beta 1 ( TGF beta 1 ) seem to exert an inhibitory activity . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
By RT PCR analysis , constitutive mRNA expression of c kit , G CSF , GM CSF , IL 1 beta , IL 6 , IL 7 , IL 8 , IL 11 , Kit ligand ( KL ) , LIF , M CSF , MIP 1 alpha , TGF beta , and TNF alpha was demonstrated in both cell lines , with L87 / 4 a more potent cytokine producer than L88 / 5 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Thus , we further determined the presence in the PBMNC conditioned medium ( CM ) of inhibitory cytokines ( macrophage inflammatory protein 1 alpha [ MIP 1 alpha ] , transforming growth factor beta 2 [ TGF beta 2 ] , interferon gamma [ IFN gamma ] , and tumor necrosis factor alpha [ TNF alpha ] ) and stimulatory cytokines ( granulocyte macrophage colony stimulatory factor [ GM CSF ] , interleukin 3 [ IL 3 ] , and stem cell factor [ SCF ] ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Receptor transmodulation between GM CSF , IL 3 , and stem cell factor ( or kit ligand ) was not detected on CD34+ cells in 48 hour cultures . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We have used a sensitive bioassay to investigate production of granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin ( IL ) 3 , IL 6 and stem cell growth factor ( SCF ) , by normal and AA stroma in long term bone marrow culture ( LTBMC ) . ^^^ All normal stroma populations produced G CSF and GM CSF , 93 % produced IL 3 , 80 % produced IL 6 , and 70 % produced SCF . ^^^ Similarly , all AA stroma produced G CSF and GM CSF , and 71 % produced SCF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Plasma levels of granulocyte colony stimulating factor ( G CSF ) , granulocyte / monocyte colony stimulating factor ( GM CSF ) , interleukin 6 ( IL 6 ) , stem cell factor ( SCF ) and macrophage inhibition factor alpha ( MIP 1 alpha ) during the transplant procedure were similar whether or not accessory cells were given . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Furthermore the stimulatory effect of the P 1 fraction on DNA synthesis in human melanocytes was neutralized by antibodies to GM CSF , but not to basic fibroblast growth factor or stem cell factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
More than moderate levels of RNA for IL 1 alpha were expressed in six of six cell lines , IL 1 beta in four , IL 6 in six , IL 7 in five , IL 10 in six , G CSF in six , GM CSF in six , SCF in six , MIP 2 beta in two , and LIF in six . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) was a weak growth stimulant , however the combination of this factor with GM CSF or IL 3 was synergistically stimulatory . ^^^ These results suggest that leukemic transformation of PMF occurs mainly at the level of myeloid stem cell , and that GM CSF , IL 3 , G CSF and SCF are major growth factors for the blast cells in these cases . . ^^^ Stem cell factor ( SCF ) was a weak growth stimulant , however the combination of this factor with GM CSF or IL 3 was synergistically stimulatory . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Constitutively expressed cytokines in this growth phase include G CSF , SCF , LIF , M CSF , IL 6 , and IL 11 , while GM CSF , IL 3 , TGF beta 2 and OSM were not detected in the growth medium . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
A combination of four growth factors ( GF ) including SCF , IL 3 , IL 6 and GM CSF was identified as the optimal combination for expanding clonogenic progenitor cells in MDS bone marrow liquid cultures . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Culturing sorted CD34+ LFA 1+ cells in single cell per well assays in medium supplemented with SCF , Epo , IL 3 , Il 6 , GM CSF , and G CSF revealed that they gave rise to dispersed macrophage like colonies , supporting the notion that CD34+LFA 1+ cells indeed consist of a mature committed cell population . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Interestingly , none of them secreted GM CSF , SCF or IL 3 , which are cytokines reputed for their ability to stimulate hematopoietic progenitors , and strikingly , only two of them were able to produce detectable levels of G CSF in culture supernatants despite the propensity of all of them to favor granulocyte differentiation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In contrast , cytokines such as IL 1 , IL 2 , IL 3 , IL 4 , IL 6 , G CSF , GM CSF , or SCF ( c kit ligand ) altered neither the proliferation capacity nor the phenotypical constellation of LW / SO cells ( each tested alone ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We also show that heparin significantly potentiates the megakaryocytopoietic activity of C Mpl ligand and interleukin ( IL ) 6 but not IL 3 , GM CSF , SCF , and Epo . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
However , in vitro stimulation of these progenitor cells using IL 3 , GM CSF , erythropoietin and steel factor ( SF ) revealed that , after 48 h in suspension culture , up to 30 % of the CD34+ cells were in S / G2m phase . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We also used the progenitor assay to screen several human and murine recombinant cytokines for cross reactivity to swine bone marrow cells , including interleukin 3 ( IL 3 ) , IL 6 , Il 11 , granulocyte and granulocyte macrophage colony stimulating factors ( G CSF and GM CSF ) , c kit ligand ( also called mast cell growth factor [ MGF ] ) , and erythropoietin ( Epo ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
TPO significantly enhanced the cytokine induced growth of AML cells in a substantial fraction of cases responsive to GM CSF , IL 3 , IL 6 or SCF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Isolated CD34+lin+ cells formed increased numbers of colony forming cells ( CFC ) when flt 3l was added together with IL 3 , IL 6 , G CSF , GM CSF or c kit ligand ( KL ) , or with the combination of IL 3 and KL . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Progenitors which responded to IL 3 , GM CSF , G CSF , M CSF and SCF were then assayed in soft agar cultures . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Cells grown in SCF also can be maintained in GM CSF but not vice versa . ^^^ Stem cell factor removal results in apoptosis and a decline in viability which can be restored partially by re addition of SCF , GM CSF , or co culture with adherent marrow stromal cells . ^^^ Stem cell factor removal results in apoptosis and a decline in viability which can be restored partially by re addition of SCF , GM CSF , or co culture with adherent marrow stromal cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
AML cells were induced to proliferation by G CSF , GM CSF , IL 3 , IL 1 beta and SCF to a different extent . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
FL promotes the growth of clonogenic myeloid progenitor cells in the presence of other cytokines known to be active on myeloid progenitors , including GM CSF , interleukin 3 ( IL 3 ) , kit ligand ( KL ) , M CSF and G CSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Cells from a single blast colony were separated into two aliquots and replated in secondary methylcellulose cultures containing SCF and IL 7 for B cell lineage and SCF , IL 3 , G CSF , GM CSF and EPO for myeloid lineage . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In five cases CD34+ selected cells grown in a stroma free long term bone marrow culture system with the addition of stem cell factor , interleukin 3 , interleukin 6 and GM CSF every 48 h , showed a 15 ( + / 15 ) and 31 ( + / 21 ) mean colony forming unit granulocyte / macrophage fold increase on cultures at days 7 and 14 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In methylcellulose assays rhMIP 1 alpha had no consistent effect on colony / cluster formation in the presence of either GM CSF + SCF or HPCM . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Pluripotential hematopoietic stem cells are most influenced by IL 3 , GM CSF , and stem cell factor while committed progenitor cells are regulated by variable concentrations of GM CSF , G CSF , M CSF , IL 5 , Epo , and Tpo . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We propose that these unique plasma , which do not contain elevated level of IL 6 , IL 3 , GM CSF , IL 1 beta , erythropoietin or SCF , probably contain high levels of TPO . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Peripheral blood blast cells were cultured in a clonogenic assay at three cell concentrations and with the following combinations of growth factors : no added growth factor ( NF ) , G CSF , GM CSF , Kit ligand ( KL ) , G CSF + KL , GM CSF + KL , and G CSF + GM CSF + KL . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
No significant ( < 10 pg / ml ) GM CSF , SCF , LIF , MIP 1 alpha , IL 2 , IL 4 , IL 11 , IL 12 , IL 13 , IL 15 , sIL 2R , or IFN gamma was detected . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CTS cells showed no significant proliferative response to the cytokines , IL 2 , IL 3 , IL 6 , IL 11 , GM CSF , G CSF , EPO , SCF , but were induced to differentiate to the T cell , B cell , erythroid or megakaryocytic lineage in the presence of particular cytokines . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
First , CD34+ cells ( 2 MM , 4 NHL ) were grown for 14 days in 5 ml of IMDM plus 12 . 5 % horse serum ( HS ) , 12 . 5 % fetal calf serum ( FCS ) and a commonly used combination of cytokines : IL1alpha , IL 3 , IL 6 , SCF , GM CSF , G CSF ( 10 ng / ml each ) and EP ( 4 UI / ml ) . ^^^ Two cultures ( 1 MM , 1 NHL ) were carried out for 14 days in 20 ml of 10 Vivo 10 medium , 2 % human serum , IL1alpha , IL 3 , IL 6 , SCF , GM CSF , G CSF ( 6 ng / ml each ) and EP ( 2 UI / ml ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CD34+ purified cells , were cultured in suspension with 6 combined hematopoietic growth factors ( IL1beta , IL 3 , IL 6 at 100 U / ml and G CSF , GM CSF and stem cell factor at 10 ng / ml of each ) for up to four weeks . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Combination of SCF , IL 6 , IL 3 , and GM CSF increases the mitotic index in short term bone marrow cultures from acute promyelocytic leukemia ( APL ) patients . ^^^ This study examined the proliferative ability of the recombinant human growth factors stem cell factor ( SCF ) , interleukin 6 ( IL 6 ) , interleukin 3 ( IL 3 ) , and granulocyte macrophage colony stimulating factor ( GM CSF ) to determine if they would provide a consistent improvement over the standard cytogenetic culturing techniques in terms of mitotic index ( MI ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In contrast , in mice treated with either GM CSF , GM CSF plus IL 4 , c kit ligand ( c kitL ) , or G CSF , class II+ CD11c+ cells were not significantly increased . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Optimal outgrowth was achieved by initially culturing CD34+ cells for 5 days in medium containing GM CSF , MGF and TNF alpha . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In this study , the effects of c kit ligand ( KL ) and flt 3 ligand ( FL ) were compared in the presence of IL 3 , GM CSF , and erythropoietin ( 3 / GM / EPO ) , using frequent medium exchange cultures of human bone marrow mononuclear cells ( BMMNC ) and CD 34 enriched cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Bronchial myofibroblasts grown from bronchial biopsies were capable of producing GM CSF , interleukin 6 ( IL 6 ) , interleukin 8 ( IL 8 ) , and stem cell factor ( SCF ) constitutively . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Platelet coated cells decreased dramatically after several days of incubation in a serum free medium supplemented with stem cell factor , IL 3 , IL 6 , and / or GM CSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
When bone marrow ( BM ) cells were cultured with GM CSF , IL 3 and SCF in a semisolid system , the colonies were exclusively eosinophilic . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Combinations of cytokines such as hepatocyte growth factor , interleukin ( IL ) 3 , IL 11 , kit ligand , GM CSF and erythropoietin were used to study the in vitro radiation dose response of human cord blood CD34+ hematopoietic progenitor cells using clonogenic survival assays . ^^^ With the combination of IL 3 , GM CSF , kit ligand and HGF , D 0 values were not significantly altered and ranged between 1 . 61 and 2 . 60 Gy . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Short term mono DC cultures initiated with GM CSF + tumor necrosis factor ( TNF ) + stem cell factor ( SCF ) exhibited increases in IL 6 level until day 11 ( peak DC growth ) . ^^^ Long term mono DC cultures sustained with GM CSF + TNF + SCF contained the highest IL 6 levels ( 671 pg / ml ) on day 11 . ^^^ Anti IL 6 profoundly inhibited cell proliferation associated with DC hematopoiesis when added on days 0 , 2 and 5 to GM CSF + TNF + SCF cultures , indicating that various stages of mono DC development rely on IL 6 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
PV hematopoietic progenitor cells also exhibit increased sensitivity to the hematopoietic growth factors GM CSF , IL 3 , and SCF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We observed no significant alterations in bone marrow stromal cell proliferation or the production of IL 6 , GM CSF , soluble c kit ligand and TNF alpha following HCMV replication in either stimulated lipopolysaccharide ( LPS ) or unstimulated conditions . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
However , optimal yields of colonies containing cells of both Mk and non Mk lineages required the addition of other growth factors , of which a combination of IL 3 , IL 6 , GM CSF and Steel factor ( SF ) + / TPO was the best of those tested . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Indeed , it has been shown that tyrosine kinase dependent stimulation of CD34+ hematopoietic cell lines with c kit ligand ( KL ) , IL 3 or GM CSF transiently activates the ability of VLA 4 to bind to VCAM 1 or FN . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In comparison with the GM CSF treated cells , the receptors for GM CSF and interleukin 3 ( IL 3 ) were down regulated and the receptors for stem cell factor ( SCF ) and TPO were up regulated in the TPO exposed cells . ^^^ A short term proliferation assay showed a stronger response of the TPO cell lines to erythropoietin , GM CSF , IL 3 , PIXY 321 , SCF and TPO than the GM CSF cell lines . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
After positive selection , CD34+ cells from leukapheresis products of 4 MM and 5 NHL patients and CD34+ cells from bone marrow ( BM ) of 3 healthy donors were grown in IMDM plus 12 . 5 % horse serum , 12 . 5 % fetal calf serum , IL 1alpha , IL 3 , IL 6 , SCF , GM CSF , G CSF ( 10 ng / ml each ) , and EP ( 4 UI / ml ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The expression patterns of high affinity receptors for G CSF , GM CSF , stem cell factor ( SCF ) , and the ligand for flk 2 on CD38hi and CD 38 / dim PB CD34+ cells were determined by binding of 125I labeled ligand and autoradiography . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Quantitation by specific ELISA and by bioassays detected high amounts of G CSF and GM CSF and smaller quantities of IL 1beta , M CSF and SCF in 5637 CM ; the concentration of IL 3 was below the detection level of the ELISA , if present at all . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Peripheral blood CD34+ cells were cultured in serum free medium with interleukin 3 ( IL 3 ) , stem cell factor ( SCF ) , and various combinations of thrombopoietin ( TPO ) , IL 6 , GM CSF , and / or G CSF . ^^^ The cytokine combination of IL 3 , SCF , TPO , IL 6 , GM CSF and G CSF produced the greatest number of granulocytes and CFU GM . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The effects of recombinant thrombopoietin ( TPO ) alone and in combination with erythropoietin ( EPO ) and early acting cytokines such as interleukin 3 ( IL 3 ) , stem cell factor ( SCF ) and GM CSF on highly purified mobilized human CD34+ progenitor cells were studied in a serum depleted culture system . ^^^ The addition of TPO to early acting cytokines ( EPO , GM CSF , SCF and / or IL 3 ) not only increased the overall megakaryocyte expansion , but also generated a different maturation pattern of the CD41+ megakaryocyte progenitors . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Bone marrow cells from male B6D2F1 / J mice were cultured for up to 28 days in serum free medium in the absence or presence of stem cell factor ( SCF ) , GM CSF or a combination of the two factors . ^^^ Compared to initial seeding levels , the presence of SCF and GM CSF increased total cell numbers 90 fold and GM CFC numbers 42 fold over a 21 28 day culture period . ^^^ Although spleen colony forming unit cells did not increase , they were maintained at initial seeding levels over a 21 day period in the presence of SCF and GM CSF . ^^^ In lethally irradiated mice , survival enhancement and hematologic reconstitution were optimum with cells cultured for only seven days : survival at six months was 100 % with cells cultured in SCF plus GM CSF or SCF alone , compared to 50 % with cells cultured with only GM CSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Proliferation of most ( n = 13 17 ) , but not of all cell lines was significantly enhanced by GM CSF , IL 3 , PIXY 321 , SCF and IFN gamma . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
TPO significantly enhanced the cytokine induced growth of AML cells in a substantial fraction of cases responsive to GM CSF , IL 3 , or SCF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The RT PCR investigation evidences the mRNA encoding for bFGF , IL 7 , GM CSF , and SCF ( c kit ligand ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
By contrast , M CSF ( 1000 U / ml ) , GM CSF ( 100 ng / ml ) , IL 1 , IL 3 , IL 6 and stem cell factor ( 10 ng / ml ) did not stimulate PAF production . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Moreover , GM CSF in the presence of SCF and IL 3 potentiates the HPP CFC formation ( i . e . , an increase of 3 . 1 fold compared to the effect induced by SCF+IL 3 ) with strong inhibitory effects on the number and size of megakaryocyte colonies . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Using ELISA and RIA , we studied , in the supernatant of cells from CDCL , the time course of interleukins 3 and 6 ( IL 3 , IL 6 ) , stem cell factor ( SCF ) , granulocyte macrophage , granulocyte and macrophage colony stimulating factors ( GM CSF , G CSF and M CSF ) , macrophage inflammatory protein 1alpha ( MIP 1alpha ) and transforming growth factor beta 1 ( TGF beta 1 ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Addition of HGFs increased the median TE 1 . 8 fold with stem cell factor alone and 2 . 6 fold with SCF , interleukin 3 and GM CSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The proliferative effects of SP correlate with the induction of positive hematopoietic growth factors such as IL 3 , IL 6 , GM CSF and c kit ligand and the inhibitory effects by NK A correlate with the induction of two negative hematopoietic regulators , MIP 1 alpha and TGF beta . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Addition of SCF ( 50 ng / ml ) to methyl cellulose cultures stimulated with maximal concentrations of G CSF , GM CSF , interleukin 3 and erythropoietin significantly increased the growth ( mean + / SE ) of CFU Mix ( 7 . 7 + / 1 . 7 versus 2 . 4 + / 0 . 6 , p < or = 0 . 0001 ) , BFU E ( 47 + / 10 versus 32 + / 6 , p < or = 0 . 002 ) and CFU GM ( 173 + / 31 versus 112 + / 20 , p < or = 0 . 0001 ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Interestingly , the combination of TPO with cytokines known to have burst promoting activity ( BPA ) , including IL 3 , GM CSF , IL 9 , and SCF , increased the number of BFU E and CFU Mix in the presence of Epo . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The effect of different expansion protocols on the expression levels of CD49dw / CD29 ( VLA 4 ) , CD11a / CD18 ( LFA 1 ) , CD 31 ( PECAM 1 ) , CD 44 , and CD 34 was determined after cord blood CD34+ cells were cultured for defined periods with the following : 1 ) A growth factor mix ( GFmix ) containing interleukin ( IL ) 1 , IL 3 , IL 6 , kit ligand ( KL ) , G CSF , GM CSF , and erythropoietin ( Epo ) ; 2 ) IL 3 + KL ; and 3 ) HS 5 ( a human stromal cell line supernatant ) + KL . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Prior to retroviral infections , CD34+ CB cells were stimulated for 60 h in a serum free medium containing a DC differentiation inducing cytokine cocktail : stem cell factor ( SCF ) , granulocyte / macrophage colony stimulating factor ( GM CSF ) , tumor necrosis factor alpha ( TNFalpha ) , and transforming growth factor beta 1 ( TGF beta 1 ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Using a combination of GM CSF , SCF , flk 2 / flt 3 ligand , and IL 4 , dendritic cells ( DC ) have been generated in vitro from the adherent fraction of mononuclear cells isolated from the blood of patients with MM . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Functionally distinct apoptotic schedules were associated with different phases of DC development when multipotent CD34+ progenitor cells were treated with GM CSF + TNF + / SCF ( c kit ligand ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
An increase in IL 3 + GM CSF + SCF responsive colony forming unit ( CFU ) ( 1 . 7 10 10 ( 4 ) + / 9 . 4 10 10 ( 3 ) and 1 . 6 10 10 ( 5 ) + / 7 . 7 10 10 ( 4 ) respectively ) was also observed as compared with input values ( 1 . 5 10 10 ( 4 ) + / 1 10 10 ( 4 ) and 2 . 3 10 10 ( 4 ) + / 8 . 9 10 10 ( 3 ) respectively ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
A million of lung mast cells generated 41 . 4 pg ( 7 . 0 273 . 6 ) ( median with range ) of TNF alpha , 252 . 6 pg ( 158 . 7 3 , 652 ) of GM CSF and 735 pg ( < 10 2 , 750 ) of IL 5 24 h after activation with SCF and anti IgE . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Interestingly , AS E 2 cells are strictly dependent on EPO for their growth and survival ; other cytokines including GM CSF , stem cell factor , or IL 3 can not support the growth of this cell line . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
WTKit cells were able to grow in the presence of the human ligand for Kit , stem cell factor ( SCF ) , but displayed reduced growth and clonogenic potential in either SCF or GM CSF compared with the parental cells in GM CSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We observed that the best yielding in CD14+ cells was obtained by adding SCF and GM CSF into RPMI 1640 completed medium and by using Teflon bags as culture containers . ^^^ For DC the best association of growth factors combined GM CSF , IL 4 and SCF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
HPC were expanded in the presence of interleukin ( IL ) 3 , IL 6 and stem cell factor ( SCF ) ( days 1 7 ) and subsequently induced by IL 4+ GM CSF ( days 8 26 ) to differentiate to Langerhans type cells ( pLC ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
METHODS : Bone marrow cells were grown in stroma free liquid cultures in the presence of SCF , IL 3 , IL 6 and GM CSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Transduction was performed with fetal bovine serum ( FBS ) or autologous plasma , IL 3 , GM CSF , IL 6 , and SCF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Fused sIL 6R / IL 6 produced a marked stimulation of cell expansion and a marked increase in the number of colony forming units when subsequently plated in semi solid medium with IL 3 , GM CSF , SCF and erythropoietin . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Ligand induced activation of HRMCs through immunoglobulin ( Ig ) E R or SCF R ( c kit ) resulted in histamine secretion ( control : < 10 % ; alphaIgE , 1 microg / mL : 50 . 12 + / 5 . 18 % ; rhSCF , 100 ng / mL : 29 . 24 + / 22 . 39 ) , whereas recombinant C5a , erythropoietin ( EPO ) , IL 1 through 10 , and GM CSF exerted no effects . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Long term coculture studies have shown that BMEC monolayers are unique type of endothelium and can support long term proliferation of hematopoietic progenitor cells particularly megakaryocytic and myeloid progenitor cells by constitutive elaboration of lineage specific cytokines such as G CSF , GM CSF , M CSF , Kit ligand , IL 6 , FLK 2 ligand , and leukemia inhibitory factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We have compared the effects of AcSDKP , Thymosin beta 4 ( Tbeta 4 ) , MIP1alpha and TGFbeta on acute myeloid leukemia ( AML ) and B lineage acute lymphoid leukemia ( B ALL ) cells using liquid cultures in the presence of GM CSF , IL 3 and SCF for AML cells and IL 3 and IL 7 for ALL cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In contrast , marked differences were not found in the levels of interleukin 3 ( IL 3 ) , tumor necrosis factor alpha ( TNF alpha ) , stem cell factor ( SCF ) , granulocyte / macrophage colony stimulating factor ( GM CSF ) , or interferon gamma ) ( IF gamma ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The expansion of bone marrow progenitor cells in a ' stroma free ' long term liquid culture system supplemented with SCF , IL 3 , IL 6 and GM CSF from 19 transplanted patients was significantly reduced compared to normal controls . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We investigated the increase in total nucleated cells , colony forming cells ( CFC ) , CD34+ cells and LTC IC by limiting dilution after a 14 day culture of CB CD34+ cells ( 5 10 10 ( 3 ) / ml ) with SCF , IL 3 , IL 6 , GM CSF and G CSF all at 10 ng / ml . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Therefore , four combinations of SCF , Flt 3 L , IL 3 , erythropoietin and GM CSF as well as three media were evaluated for their capacity to amplify hematopoietic progenitors . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In order to investigate the sensitivity of malignant target cells to lysis by LAK cells according to their clonogenic capacities and their position along the maturation pathway , we compared clonogenic and chromium release cytotoxicity assays performed on human hematopoietic cell lines using Effector : Target ratios of 1 : 1 , 3 : 1 , 6 : 1 , 12 : 1 , 24 : 1 , 48 : 1 and 96 : 1 , and studied the sensitivity of HL 60 and U 937 human cell lines after exposure to different factors including GM CSF , SCF , IFN , Retinoic acid ( RA ) , DMSO , and TPA which are able to recruit cells into the cell cycle or to induce cell differentiation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The polymerase chain reaction based differential display method ( DDRT PCR ) was used to identify mRNAs differentially expressed during the maturation of human CD34+ progenitor cells stimulated to differentiate in vitro towards granulomonocytic or erythroid lineages with a mixture of hemopoietins ( kit ligand + interleukin 3 + GM CSF in the absence or presence of erythropoietin , respectively ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
TGF beta 1 was found to be an absolute requirement for in vitro LC development under serum free conditions upon stimulation with the classical DC growth and differentiation factors GM CSF , TNF alpha and SCF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In the present study , abnormal hematologic profiles were induced with erythropoietin ( EPO ) , recombinant murine stem cell factor ( rm SCF ) , granulocyte macrophage stimulating factor ( GM CSF ) , and cyclophosphamide ( CP ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We have investigated the growth and proliferation of purified populations of hematopoietic progenitor cells isolated from Nf 1 knockout mice in response to the cytokines interleukin ( IL ) 3 and stem cell factor ( SCF ) , as well as to GM CSF . ^^^ We found abnormal proliferation of both immature and lineage restricted progenitor populations , and we observed increased synergy between SCF and either IL 3 or GM CSF in Nf 1 / progenitors . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Cells with a dendritic morphology and expressing HLA DR , CD1a , S 100 and CD 83 were maximally expanded under serum free conditions with the addition of SCF , GM CSF , TNF alpha , TGF beta and Flt 3 ligand ( fold increase of CD1a+ cells = 102 + / 32 after 2 weeks of culture ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Conversely , production of GM CSF , SCF , M CSF and MCP 1alpha was elevated in NS compared with BM fibroblasts . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Optimum colony growth was obtained when stem cell factor ( SCF ) and granulocyte macrophage colony stimulating factor ( GM CSF ) were used as growth stimuli of colony forming units ( CFU ) . ^^^ Loss of long term culture initiating ability could be demonstrated in this system with lineage negative marrow cells expanded ex vivo with SCF and GM CSF . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Then CD34+ cells isolated from cord blood were infected three times after a 48 h prestimulation with IL 3 , IL 6 , SCF or with IL 3 , IL 6 , SCF , GM CSF , Flt 3 L and TPO . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We analyzed the effect of tumor necrosis factor ( TNF ) alpha on the differentiation and viability of dendritic cells ( DC ) generated from cord blood CD34+ progenitors cultured for five days with GM CSF , Flt 3 ligand ( FL ) , and stem cell factor ( SCF ) , and then with GM CSF only [ TNF ( ) cultures ] . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In the absence of recombinant human ( rh ) SCF , anti IgE induced a median GM CSF response of 202 ( < 15 to approximately 681 ) pg / 10 ( 6 ) mast cells / 24 h , whereas in the presence of rhSCF the median IgE dependent GM CSF release was 356 ( 152 to approximately 1216 ) pg / 10 ( 6 ) mast cells / 24 h . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
FL acts synergistically in vitro with a number of other hematopoietic growth factors such as IL 3 , IL 6 , IL 11 , IL 12 , KIT Ligand and GM CSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We investigated the co stimulatory activity of 14 cytokines ( interleukin 1 to 8 , IL 10 , IL 13 , INF alpha , TNF alpha , GM CSF and SCF ) on the proliferation of CD 40 activated follicular lymphoma cells in comparison to tonsillar GC B cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
ELISA confirmed the presence of one or several of the following cytokines in the CM of carcinoma cell lines : GM CSF , G CSF , macrophage CSF ( M CSF ) , stem cell factor ( SCF ) and IL 6 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The cell lines produced G CSF ( 1 / 11 cell lines ) , GM CSF ( 2 / 11 ) , SCF ( 2 / 11 ) , M CSF ( 6 / 11 ) . and interleukin 6 ( 9 / 11 ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CD34+ cells purified from leukapheresis product were seeded at 1 10 10 ( 4 ) cells / mL in complete medium supplemented with GM CSF , TNF alpha , IL 4 , c kit ligand , and flt 3 ligand ( FL ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CD34+ cells were cultured in serum free medium containing stem cell factor ( SCF ) , GM CSF , and interleukin 3 ( IL 3 ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Moreover , SNAP 23 was induced in response to a wide variety of cytokines , including IL 2 , IL 3 , IL 5 , IL 10 , stem cell factor , G CSF , GM CSF , leukemia inhibitory factor , and erythropoietin . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Marrow stromal cells were evaluated several months after autologous BMT for their capacity to support both normal hemopoiesis and secrete the main growth factors involved in its control , G CSF , GM CSF , IL 3 and SCF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Among the cytokines , IL 3 , IL 6 , and IL 7 , but not IL 2 , IL 4 , SCF , GM CSF induces RAG activation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Clonogenic assays in methylcellulose showed an increased frequency and proliferation of colony forming unit dendritic cells ( CFU DC ) in circulating CD34+ cells , compared to that of BM CD34+ precursors in response to GM CSF and TNF alpha with or without SCF and FLT 3L . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Supernatants of IL 1alpha stimulated EC potentiated the effects of an association of IL 1 , IL 3 , IL 6 , LIF , SCF , Flt 3 ligand , TPO , G CSF , GM CSF , M CSF and IL 11 on the proliferation of hematopoietic progenitors suggesting that EC may produce other soluble growth factors potentiating the action of the above set of cytokines . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The abilities of stromal cells to produce hematopoietic growth factors , including G GSF , GM CSF , IL 6 and SCF , are either normal or elevated in the majority of patients . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We investigated the increase in total nucleated cells , colony forming cells ( CFC ) , CD34+ cells and long term culture initiating cells ( LTC IC ) by limiting dilution after a 14 day culture of CB CD34+ cells ( 5 10 10 ( 3 ) / ml ) with SCF , IL 3 , IL 6 , GM CSF and G CSF all at 10 ng / ml . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Purified human CD34+ cells were cocultured with PMVECs for 7 days in the presence of optimal concentrations of granulocyte / macrophage colony stimulating factor ( GM CSF ) + interleukin ( IL ) 3 + IL 6 + stem cell factor ( SCF ) + Flt 3 ligand . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In vivo administration of G CSF reduced the adherence capacity of CD34+ cells to normal BM stroma ; in vitro incubation with SCF or IL 3 enhanced the expression of CD49d on CD34+ cells , while GM CSF reduced the expression of CD62L . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
No increase in GM colony formation was observed , either , when FL was used in combination with other GFs , such as mast cell growth factor ( MGF ) , granulocyte colony stimulating factor ( G CSF ) , GM CSF or interleukin 3 ( IL 3 ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The addition of growth factors such as G CSF , GM CSF , IL 3 , IL 6 , or Stem Cell Factor did not affect the growth . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Similarly , high BMP 9 concentrations dramatically inhibited primary colony formation induced by SF+IL 11 / 12 , whereas in the presence of TGF beta 1 neutralizing Ab only 3 ng or more BMP 9 per ml stimulated both the time of colony appearance , the colony size and colony numbers in the presence of IL 3 , M CSF , GM CSF , SF , SF+Flt3 L , SF+IL 3 , SF+IL 11 / 12 or IL 3+SF+IL 11 / 12 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The cytokines investigated included interleukin ( IL ) 1alpha , IL 1beta , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , IL 7 , IL 8 , IL 10 , IL 13 , TNF alpha , TNF beta , IFN gamma , TGF beta 2 , G CSF , GM CSF and SCF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Treating HAS 303 cells with SP increased the intracellular Ca2+ concentration and stimulated production of G CSF , GM CSF , SCF and IL 6 , but not IL 1alpha , IL 1beta and TNF alpha , but did not enhance proliferation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
An increased production of G CSF , GM CSF , IL 8 , IL 6 , IL 1alpha , IL 11 , MIP 1alpha and SCF and no production of LIF , TNF alpha , TPO and IL 3 by 2 Gy irradiated TrHBMEC was observed . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In contrast , in LFSS patients , low levels of TGF beta and the increased release of GM CSF and SCF maintain the intense erythropoiesis in response to higher erythropoietic stress , in these more severe patients . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Using the panel of specific antibodies the expression of cytokine receptors ( IL 1 , 2 , 3 , 6 , 7 , 8 , 10 , SCF , gp 130 , G CSF , GM CSF , EPO ) and Fas , Pgp and 24 34 kD multidrug resistance associated protein were examined . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We describe the in vitro effect of pegylated recombinant human MGDF ( PEGrHuMGDF ) alone and in combination with other haemopoietic growth factors ( G CSF , GM CSF , IL 3 , IL 6 , erythropoietin , SCF ) on megakaryopoiesis in bone marrow from 11 normal subjects and 19 patients with aplastic anaemia ( AA ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In this study we have investigated the effect of the early acting haemopoietic cytokines flt 3 ligand ( FL ) , IL 3 and GM CSF on the SCF dependent differentiation of mast cells from cord blood mononuclear cells . ^^^ The appearance of mast cells was attenuated when the cells had been in culture with IL 3 or GM CSF prior to culture in SCF , compared to cultures exposed to SCF alone for 7 weeks . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
DESIGN AND METHODS : To achieve long term expression of a specific transgene in APCs , G CSF mobilized peripheral blood CD34+ cell populations were retrovirally transduced with the bacterial nlsLacZ , a marker gene used here as a model , in the presence of IL 3 , IL 6 , GM CSF and SCF prior to being induced to differentiate into dendritic and macrophage cells by GM CSF and TNF a . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
G CSF , GM CSF , IL 3 , M CSF , PIXY 321 , SCF ) had synergistic or additive mitogenic effects . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Although GM CSF had minimal effects on the survival or proliferation of primitive progenitors when added alone , the cytokine enhanced stem cell factor ( SCF ) induced cell cycle entry in the first generation . ^^^ The effect was not observed when cells were incubated sequentially with SCF and GM CSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
By adding TPO to all possible combinations of GM CSF , IL 3 , and c kit ligand ( CKL ) in a suspension culture system , we found a significant increase in both relative and absolute numbers of cells in cultures containing TPO of the megakaryocytic lineage and CD34+ cells after 14 days of culture . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Purified CD34+ cells expanded exponentially and produced 800 to 4000 fold more nucleated cells , 300 to 3500 fold more GM CFU and 10 to 80 fold more HPP CFC in stroma free suspension culture with interleukin 1 ( IL 1beta ) , IL 3 , IL 6 , GM CSF and stem cell factor ( SCF ) , than did the starting cell input . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Endogenous serum thrombopoietin ( TPO ) and various cytokines including erythropoietin ( EPO ) , interleukin ( IL ) 3 , IL 6 , IL 11 , granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) and stem cell factor ( SCF ) levels were measured in five patients with acute promyelocytic leukaemia ( APL ) during all trans retinoic acid ( RA ) treatment . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Then they were cultured , both in agar and in limiting dilution liquid culture , in the presence of 5637 cell line supernatant ( containing GM CSF , G CSF and interleukin 1 ) , stem cell factor ( SCF ) and interleukin 3 ( IL 3 ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Cord blood CD34+ cells were then cultured for 14 d with IL 3 , IL 6 , SCF , G CSF and GM CSF , each at 10 ng / ml . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We have tested the efficacy of pig specific cytokines ( PSC ) ( IL 3 , SCF , GM CSF ) in the mobilization of porcine bone marrow ( BM ) progenitors in vivo ( 1 ) in the pig and ( 2 ) in baboons that underwent a conditioning regimen and porcine BM transplantation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The cytokines IL 3 , IL 4 , stem cell factor ( SCF ) , GM CSF , and insulin all induced the rapid and transient activation of PKB in responsive cell lines . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We could demonstrate in HUAEC that stimulation with IL 1alpha and TNF alpha led to the mRNA expression of basic fibroblast growth factor ( bFGF ) and vascular endothelial growth factor ( VEGF ) which are crucial in the process of angioneogenesis and atherosclerosis as well as of the granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) and stem cell factor ( SCF ) which are main growth factors in haematopoiesis . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Using KSOM as the culture medium , the effects of stem cell factor ( SCF ) and granulocyte macrophage colony stimulating factor ( GM CSF ) were studied . ^^^ The combination of SCF with GM CSF had no significant effect on the percentage survival of type A spermatogonial cells . ^^^ These data indicate that SCF and GM CSF play a role in the regulation of survival and / or proliferation of type A spermatogonia . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
However , these enriched cells ( CD56+ , CD 45 , CD 34 ) did not give rise to hemopoietic cells under the culture conditions used , i . e . in the presence of the growth factors IGF 1 , bFGF , SCF , IL 3 , and GM CSF Together , our data do not support the hypothesis that EDC are totipotent mesenchymal progenitors giving rise to hemopoietic cells . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
However , addition of human SCF , G CSF , GM CSF , LIF , MIP 1alpha and IL 6 in concentrations found in human marrow stroma conditioned medium to AFT 024 non contact cultures increased LTC IC maintenance to 72 % at 5 weeks . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The present studies showed a significant baseline level of apoptotic cells in short term ( 5 day ) cultures of normal CD 34 ( + ) cells containing GM CSF plus stem cell factor ( SCF ) , and a markedly greater level of apoptosis in cytokine deficient cultures . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In contrast , the presence of both SCF and GM CSF allows for the continuous survival and expansion of hematopoietic progenitor cells in culture , as well as favoring their terminal differentiation along granulocytic and monocytic pathways . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
To clarify this point , the serum level of G CSF was measured using an ELISA , and various other cytokines , including GM CSF , macrophage CSF ( M CSF ) , SCF , IL 6 , IFN gamma , and soluble IL 2 receptor ( IL 2R ) , were tested for comparison in children receiving conventional or high dose chemotherapy and autologous transplantation with unmanipulated or purified PBSC . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Although adenoviruses can infect nondividing cells , we observed that a combination of growth factors ( GM CSF , IL 3 , stem cell factor ) was required for efficient transduction in order to maintain AML blast cell viability . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Despite this low level expression , flt 3 L exerted synergistic effects with a combination of c kit ligand , IL 3 , IL 6 , GM CSF and G CSF , mainly to induce proliferation of CD34+ / CD38 cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The improvement of the procedure of hematopoietic cells transplantation and its efficacy is caused by the stimulation of the hematopoiesis by using the combination of cytokines ( G CSF , GM CSF , IL 3 , SCF ) and purging of the hematopoietic cells obtained from the cellular apheresis . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Finally , purified CD 34 ( + ) CD 40 ( + ) blood cells stimulated an alloreactive T cell response in MLC , were enriched in granulocytic , monocytic , and dendritic precursors , and generated high numbers of DC in 11 14 d liquid cultures with GM CSF , SCF , TNF alpha and FLT 3L . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The colonies grown from progenitor cells were cultured in methylcellulose either in the presence or absence of growth factors , including GM CSF , stem cell factor , and IL 3 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Single CD34+ cells from the CD 123 subsets were cultured in a cytokine cocktail of stem cell factor , interleukin 3 ( IL 3 ) , IL 6 , GM CSF , erythropoietin , insulin like growth factor 1 , and basic fibroblast growth factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Since SF DC have differentiated in vivo in the presence of proinflammatory cytokines , PB DC were cocultured in the presence of IL 10 and either GM CSF , IL 1beta , TNF alpha , IL 6 , or TGF beta . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The occurrence of receptors for G CSF , GM CSF , IL 3 and SCF on marrow cells was assessed using immunofluorescent ligand binding assays . ^^^ Incubation of AML cells in serum free medium ( SFM ) + / G + GM CSF , IL 3 , SCF , G + GM CSF + SCF or IL 3 + SCF prior to S phase assessment revealed that priming permutations inclusive of SCF were most effective but also that the baseline level of S phase activity with SFM alone influenced the subsequent response to priming . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Preincubation of CD 34 ( + ) cells with stem cell factor ( SCF ) before A 23187 stimulation resulted in primed PLA ( 2 ) activity , whereas interleukin 3 ( IL 3 ) , GM CSF , and tumor necrosis factor alpha had no significant effect . ^^^ When CD 34 ( + ) cells were induced to differentiate , PLA ( 2 ) activity remained responsive to SCF for several days , but after 8 days , at which stage morphological and functional evidence of maturation was occurring , priming of PLA ( 2 ) by SCF could no longer be elicited , whereas responses to GM CSF and IL 3 had developed . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NALM 29 cells responded significantly to the proliferative stimuli of FLT 3 ligand and IL 7 , but not to GM CSF , IL 3 , IL 6 , PIXY 321 or SCF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Three lines of Tg mice were generated : one carrying porcine IL 3 and GM CSF genes only ( termed IL / GM ) and the remaining two lines carrying in addition , the soluble SCF gene ( termed IL / GM / sS ) or membrane bound SCF gene ( termed IL / GM / mS ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
DCs were derived from CD 34 ( + ) progenitors using SCF plus GM CSF plus TNFalpha . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We have investigated the serum level of stem cell factor ( SCF ) and granulocyte macrophage colony stimulating factor ( GM CSF ) in relation to a control group and commonly accepted tumour markers , such as carcinoembryonic antigen ( CEA ) and cytokeratin fragment 19 ( CYFRA 21 1 ) . ^^^ Additionally , we have defined the diagnostic sensitivity , specificity , positive predictive value , negative predictive value and receiver operating characteristics ( ROC ) curve of SCF and GM CSF in non small cell lung cancer ( NSCLC ) . ^^^ SCF and GM CSF were determined using enzyme linked immunosorbent assay ( ELISA ) , CYFRA 21 1 was measured by radioimmunoassay and CEA by microparticle enzyme immunoassay . ^^^ There were significant increases in circulating SCF and GM CSF in the lung cancer patients compared to the control group . ^^^ The diagnostic specificity of GM CSF was lower ( 65 % ) than SCF specificity ( 70 % ) , but the GM CSF predictive values were higher in relation to the predictive values of SCF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
A large number of cells with the morphological , phenotypical , and functional characteristics of DCs was generated from both c kit ( low ) and c kit ( low ) populations when cultured with a combination of cytokines ( GM CSF , tumor necrosis factor a [ TNF a ] , interleukin 7 [ IL 7 ] , IL 3 , stem cell factor [ SCF ] , and flt 3 ligand ) ; the cytokine combination studies revealed that SCF and IL 3 in addition to GM CSF and TNF a are essential for DCs to be generated from these primitive populations . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Twelve cases of human AML were tested for ability to be differentiated toward a professional APC phenotype in short term culture with addition of GM CSF and the following recombinant proteins : TNFalpha , IL 4 , CD 40 ligand , Flt 3 ligand and SCF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Also in CD34+ precursors , cultured in the presence of flt 3 ligand , c Kit ligand , GM CSF , IL 4 and TNF alpha , addition of anti CD 33 mAb prevented the recovery of mature dendritic cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The highest yield of CD1a ( + ) / CD14 ( ) cells could be obtained with stem cell factor , transforming growth factor beta , tumor necrosis factor alpha , GM CSF , and FLT 3 ligand . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
TGF beta , GM CSF , c kit ligand , and IL 7 are produced constitutively and IL 1beta , IL 6 , IL 8 , TNF alpha , IFN gamma , and MIP 1 alpha are upregulated upon stimulation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Leukemic cells were cultured in the presence or absence of various cytokines including GM CSF , SCF , TNF alpha , IL 4 , and gamma interferon . ^^^ Proliferation ( 5 / 14 ) or increased survival ( 7 / 14 ) of AML cells was observed in the presence of GM CSF , SCF , and TNF alpha . ^^^ GM CSF , SCF , and TNF alpha induced morphologic changes typical for DC and increased the expression of costimulatory and adhesion molecules . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
SCF , IL 1beta , IL 1ra and GM CSF in the bone marrow and serum of normal individuals and of AML and CML patients . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Recently , cytokines and interleukins such as SCF , GM CSF , G CSF , TGF beta , IL 6 , IL 7 , IL 8 , IL 11 have been reported to be elaborated by endothelial cells . ^^^ Our finding of the existence of mRNAs of SCF , GM CSF , IL 6 , TGF beta , IL 1 , and IL 11 in these cells was in agreement with the data reported previously . ^^^ Among these cytokines and interleukins , SCF , GM CSF , IL 6 , IL 1 , and IL 11 are haematopoietic stimulators which may be responsible for the stimulative effects on the growth of haematopoietic progenitors . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We demonstrate here that LC development from human CD34+ hemopoietic progenitor cells in response to TGF beta 1 costimulation ( basic cytokine combination GM CSF plus TNF alpha , stem cell factor , and Flt 3 ligand ) is associated with pronounced cell cluster formation of developing LC precursor cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
By day 21 , the endothelial based system receiving the FLT3L , SCF , IL 6 , and GM CSF cytokine combination contained five fold greater numbers of CD34+ than the stroma cell free culture cell system . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Enhanced antitumor effect of suicide gene therapy by SCF , GM CSF gene transfer in vivo and its immunological mechanism ] . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Peripheral blood mononuclear cells ( PBMCs ) from 15 newly diagnosed acute myeloid leukemia ( AML ) patients were cultured in fetal calf serum free media supplemented with either granulocyte / macrophage colony stimulating factor ( GM CSF ) , interleukin ( IL ) 4 and tumor necrosis factor alpha ( TNFalpha ) , or GM CSF , stem cell factor ( SCF ) , TNFalpha and transforming growth factor beta ( TGFbeta ) in order to generate leukemia derived dendritic cells ( DCs ) . ^^^ GM CSF , IL 4 plus TNFalpha was superior in 11 cases , and better results were obtained with GM CSF , SCF , TNFalpha plus TGFbeta in 3 cases . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
A significant reduction of CD 34 positive cells after incubation with a cytokine mix [ interleukin ( IL ) 1beta , IL 3 , IL 6 , stem cell factor ( SCF ) , erythropoietin ( EP ) with granulocyte macrophage / colony stimulating factor ( GM CSF , Cytok 1 ) could be achieved in all 16 cases with a CD 34 positive blast phenotype studied at diagnosis ( P < 0 . 001 ) , in 3 of 10 cases at relapse , and in 8 of 18 cases in complete remission . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
When stimulated in agar cultures by GM CSF , G CSF , M CSF , IL 3 , SCF , IL 6 , leukemia inhibitory factor ( LIF ) , IL 5 or IFNgamma , GB 2 cells generated blast colonies or colonies of maturing granulocytes and macrophages . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In physical or biological characteristics , it exhibited a unique character different from IL 3 , IL 6 , GM CSF , SCF or FLT 3 ligand those are known to have hematopoietic stem cell proliferation stimulating activity . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
MC were produced by using GM CSF and SCF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In this article we describe the differentiation and expansion of lentivirus vector marked DCs from umbilical cord blood , bone marrow , and cytokine mobilized peripheral blood CD 34 ( + ) cells in the presence of GM CSF , TNF alpha , SCF , Flt 3 , and IL 4 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
PMVEC coculture supplemented with GM CSF + IL 3 + IL 6 + SCF + Flt 3 ligand induced > 93 % of the CD 34 ( + ) CD 38 ( ) population to enter the G ( 1 ) or G ( 2 ) / S / M phase while increasing this population from 1 . 4 % on day 0 to 6 . 5 % of the total population by day 5 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Hemopoietic cells were grown in long term cultures in the presence of various combinations of early acting cytokines such as FLT 3 ligand ( FLT 3 L ) and stem cell factor ( SCF ) and the differentiating growth factors GM CSF and TNF alpha . ^^^ Conversely , FLT 3 L and SCF do not directly affect DC differentiation , but instead sustain the long term expansion of CFU DC , which can be induced to produce mature DC by GM CSF and TNF alpha . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
G CSF , IL 6 and thrombopoietin ( TPO ) each enhanced light scatter compared to control PRP , although IL 3 , GM CSF , erythropoietin and stem cell factor did not . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
RESULTS : Different combinations of HGFs , including SCF , IL 3 , IL 6 , GM CSF , Epo and Tpo increased BFU E , CFU GM , CFU MK and CD41a+ cells by 14 . 97 + / 2 . 89 fold , 14 . 46 + / 3 . 19 fold , 34 . 67 + / 4 . 62 fold and 17 . 29 + / 2 . 34 fold , respectively . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The addition of AcSDKP in short term bone marrow cultures resulted in a maximum depression of the total number of progenitor cells as well as the number of progenitor cells entering cell cycle following culture with 10 ( 12 ) to 10 ( 14 ) M AcSDKP and 10 ( 14 ) M AcSDKP when exogenous cytokines ( GM CSF , IL 3 , or SCF ) were added . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Megakaryocytopoiesis is a complex network regulated by different megakaryocyte ( MK ) stimulating factors ( i . e . , thrombopoietin [ TPO ] , stem cell factor [ SCF ] , interleukin 3 [ IL 3 ] , IL 6 , IL 11 and GM CSF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We treated human cord blood CD34+ cells with either GM CSF alone or in combination with stem cell factor and followed methylation at this locus using bisulfite genomic sequencing . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CD 38 ( ) and CD 38 ( + ) progenitors that expressed high levels of CD 34 were grown in serum deprived medium supplemented with kit ligand , flk2 / flt3 ligand , GM CSF , c mpl ligand , erythropoietin , and IL 15 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In the first phase of the study ( Protocol Refinement ) , two SOPs were assessed , by using two cell culture media ( Test A , containing GM CSF ; and Test B , containing G CSF , GM CSF , IL 3 , IL 6 and SCF ) , and the two tests were applied to cells from both human ( bone marrow and umbilical cord blood ) and mouse ( bone marrow ) CFU GM . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
RESULTS : Cultures of cord blood CD34+ cells with the combination of GM CSF + TNF alpha + SCF + FL yielded ( 27 . 18 + / 1 . 56 ) % CD1a+ cells , much higher than that ( 0 . 65 + / 0 . 38 ) % with GM CSF alone . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Despite the incapacity of SF to sustain FDC P 1 cells , the biologically active GM CSF could be recovered from all the studied stromas by treatment with high salt buffers that release non covalently bound molecules from stroma cells . ^^^ Glycosaminoglycans purified from stromas had distinct effect on the GM CSF mediated proliferation of FDC P 1 cells : those purified from S 17 and GR cells were stimulatory , whereas those obtained from SF cells were slightly stimulatory at low concentration , but inhibitory at the higher ones . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We have already shown that cytokine cocktails ( IL 1beta , IL 3 , IL 6 , SCF , GM CSF ) and / or lymphokine activated killer ( LAK ) cells can reduce the amounts of clonal , CD 34 positive mononuclear bone marrow cells ( BM MNC ) in acute myeloid leukemia ( AML ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
To assess the hematopoietic defect involved in PNH , CD34+ CD59+ ( normal phenotype hematopoietic stem / progenitor ) and CD34+ CD 59 ( PNH phenotype ) cells from PNH patients ( n = 16 ) and CD34+ CD59+ cells from healthy volunteers ( n = 10 ) were sorted as single cells into 96 well flat bottom culture plates containing culture medium supplemented with stem cell factor , interleukin ( IL ) 3 , erythropoietin , granulocyte macrophage colony stimulating factor ( GM CSF ) , G CSF , IL 6 , thrombopoietin , and Flt 3 ligand . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
However , in vitro expansion and differentiation in the presence of GM CSF , TNF alpha , Flt 3L , SCF and IL 4 resulted in a reduction in the percentage of DC expressing the transgene . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Blast cells were expanded in serum free medium supplemented with Flt3L , G CSF , GM CSF , IL 3 , IL 6 and SCF for 7 days and subsequently stimulated with Flt3L , GM CSF and TGF beta for a further 14 days , with the addition of TNF alpha for the final 48 h of culture . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The rhTpo induced proliferation of AML cells was enhanced when combined with IL 3 , GM CSF or SCF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
METHODS : Hematopoietic cells from human umbilical cord blood were cultured with G CSF , GM CSF , rIL 3 , rIL 6 , SCF and Epo in a long term culture system , the cell cycle and cellular markers were analysed dynamically . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
DCs were induced from cord blood CD ( 34 ) ( + ) cells with a combination of cytokines ( GM CSF , TNF alpha , SCF and FL ) and ( 3 ) H TdR was adopted to determine the proliferation of T cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Stem cell factor could cooperate with G CSF , GM CSF , and erythropoietin significantly increasing the numbers of CFU GM , BFU E and CFU E of CAA , SAA and normal control ' s enriched CD 34 positive MNC . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
When cultured in a semi liquid methylcellulose medium , there were more eosinophil colony forming units grown from asthmatic non adherent mononuclear cell depleted of T cells in the presence of the growth factors GM CSF , SCF and IL 3 , but not of IL 5 . 3 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In conclusion we have discovered a new function of the hemopoietic stem cell factor GM CSF , which is also a powerful arteriogenic peptide that acts via prolongation of the life cycle of monocytes / macrophages . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
By screening a panel of secreted factors , granulocyte macrophage colony stimulating factor ( GM CSF ) , interferon ( IFN ) gamma , and stem cell factor ( SCF ) were found induced by HTLV 2 in TF 1 cells . ^^^ Neutralization experiments indicated that GM CSF and IFN gamma , but not SCF , were responsible for HTLV 2 induced STAT activation , whereas anti GM CSF antibodies greatly inhibited TF 1 cell proliferation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Langerhans cells can originate in vitro from immature precursors stimulated with granulocyte macrophage colony stimulating factor ( GM CSF ) , tumour necrosis factor ( TNF ) alpha and stem cell factor ( SCF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Stem Cell Factor ( SCF ) + IL 6 and FLT 3 ligand , which have no effect on colony formation by themselves , were used to enhance the effects of IL 3 and GM CSF , respectively . ^^^ Stem Cell Factor ( SCF ) + IL 6 and FLT 3 ligand , which have no effect on colony formation by themselves , were used to enhance the effects of IL 3 and GM CSF , respectively . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Optimal growth and DC characteristics were obtained with GM CSF , FL , and SCF in 3 5 day cultures , suggesting a practical strategy for the immunotherapy of leukemia . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The two step method consists of 10 days of first step culture for the expansion and proliferation of CD34+ hematopoietic progenitor cells in the presence of SCF , IL 3 , IL 6 , G CSF , and 7 11 days of second step culture for the induction of DC in the presence of GM CSF , IL 4 and TNF alpha . ^^^ With this two step culture , the absolute number of CD1a+ cells that co expressed HLA DR , CD 80 , CD 40 and CD 58 was enhanced approximately 3 times in CB cell culture and 1 . 9 times in BM cell culture , compared with the commonly used one step culture method for the generation of DC from CD34+ cells using SCF , GM CSF and TNF alpha . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Significantly higher levels of proliferation and expansion of progenitor cells were observed in the presence of SF , IL 6 , GM CSF , and G CSF with preferential stimulation of myelopoiesis . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Although mRNA for IL 3 and GM CSF was never detected , transcripts for c kit ligand and , more commonly , its receptor were . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
When estimating the general profile of the cytokines of these thymocytes by PCR with revertase , it was demonstrated the cells in question did not express cytokines with colony stimulating activities ( SCF , IL 3 , or GM CSF ) or cytokines affecting the migration of bone marrow stem elements ( IL 2 , 4 , or 7 ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Best results were achieved when 24 h of prestimulation were followed by a 24 48 h transduction period in the presence of the CH 296 FN fragment and IL 3 , IL 11 , SCF , erythropoietin ( EPO ) , and GM CSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Bone marrow CD34+ progenitor cells stimulated with stem cell factor and GM CSF have the capacity to activate IgD B cells through direct cellular interaction . ^^^ Bone marrow CD34+ cells from RA patients that had been stimulated with stem cell factor and GM CSF for 3 4 weeks ( > 90 % CD14+ HLA DR+ cells , < 0 . 5 % CD19+ B cells , and < 0 . 5 % CD3+ T cells ; MLC ) induced the production of IgG much more effectively than that of IgM by highly purified B cells from healthy donors in the presence of IL 2 and IL 10 . ^^^ These results indicate that upon stimulation with stem cell factor and GM CSF , CD34+ progenitor cells differentiate into MLC that activate preferentially IgD B cells through direct cellular interactions to produce IgG . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Although cytokines do not appear to be centrally involved in the pathogenesis of acute leukemias , leukemic cells express receptors for many of the cytokines regulating normal hematopoiesis , particularly G CSF , GM CSF , IL 3 , and stem cell factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Here we report that Nup 98 HoxA9 , indeed mimicks Hoxa 9 plus Meis 1 coexpression it immortalizes myeloid progenitors , prevents differentiation in response to GM CSF , IL 3 , G CSF , and permits proliferation in SCF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Combinations of thrombopoietin and cytokines that act on megakaryocyte development ( stem cell factor , IL 3 , IL 6 , IL 11 , flt 3 ligand ( now known as FLT3LG ) , erythropoietin , GM CSF and G CSF were evaluated for their ability to enhance clonal growth in vitro of 10 irradiated CD 34 ( + ) megakaryocytic progenitor cells ( CFU megakaryocytes ) purified from normal human peripheral blood . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
METHOD : The CD ( 34 ) ( + ) CD ( 59 ) ( + ) and CD ( 34 ) ( + ) CD ( 59 ) ( ) cells from PNH patients and CD ( 34 ) ( + ) CD ( 59 ) ( + ) cells from normal volunteers were sorted as each single cell into a well of 96 well culture plates containing culture medium supplemented with SCF , IL 3 , Epo , GM CSF , G CSF , IL 6 , Tpo and Flt 3 ligand . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The cells were cultured for 12 days in the presence of GM CSF , SCF , FL , IL 4 and TNF alpha to get mature DC enriched population . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
BAEP 2 SV40 cell line , but not BAEP 2 W , expressed interleukin ( IL ) 1 , IL 6 , granulocyte macrophage colony stimulating factor ( GM CSF ) , granulocyte colony stimulating factor , stem cell factor and vascular cell adhesion molecule 1 mRNAs , and secreted IL 6 and GM CSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In serum free suspension cultures of CD34+ cells , elastase completely abrogated the proliferation induced by G CSF but not that of GM CSF or stem cell factor ( SCF ) . ^^^ When exposed to enzymatically active elastase , G CSF , but not GM CSF or SCF , was rapidly cleaved and rendered inactive . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
TTA inhibited AML blast proliferation in the presence of single cytokines ( GM CSF and SCF : P > 0 . 05 , 35 patients with detectable proliferation ) and a combination of cytokines ( P < 0 . 005 , n = 21 ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Cloning of canine GM CSF and SCF genes . ^^^ In order to determine the complementary DNA ( cDNA ) of canine GM CSF and canine SCF , cDNA clones were generated from lipopolysaccharide ( LPS ) stimulated peripheral blood mononuclear cells ( PBMCs ) and bone marrow cells by reverse transcription PCR amplification . ^^^ The canine GM CSF cDNA obtained in this study contains an open reading frame encoding 144 amino acid residues and has 53 75 % homology with those of human , cat , sheep , pig , cow and mouse , Canine SCF cDNA consist of an open reading frame encoding 274 amino acid residues and shares 81 92 % homology with those of human , cat , pig , cow and mouse . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The component above 10 kD combined with IL 3 , IL 6 , EPO , SCF or GM CSF further enhanced HPP CFC colony formation . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
EPO acts synergistically with several growth factors ( SCF , GM CSF , 1L 3 , and IGF 1 ) to cause maturation and proliferation of erythroid progenitor cells ( primarily colony forming unit E ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
For the work presented here , DC were differentiated from human peripheral blood mononuclear cells ( PBMC DC ) in culture medium containing GM CSF and interleukin ( IL ) 4 to ensure an immature phenotype or were derived from the KG 1 cell line ( KG 1 DC ) using a defined cytokine cocktail consisting of GM CSF , IL 4 , Flt 3 / Flk 2 ligand , thrombopoietin , stem cell factor , and tumor necrosis factor alpha ( TNFalpha ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Importantly , the maturational effects of TRAIL were observed also in primary normal CD 34 ( + ) cells , seeded in serum free liquid cultures for 4 to 8 days in the presence of SCF + GM CSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CD34+ peripheral blood progenitor cells were differentiated to immature DC in the presence of GM CSF , IL 6 / IL 6R fusion protein and stem cell factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The observed activity of SCF in marrow cultures was relatively weak , but its action may be stronger in vivo , and SCF needs to be added to GM CSF , IL 5 , and IL 3 in the list of cytokines able to stimulate eosinophil formation . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We investigated the role of three parameters : A ) the culture medium ( serum free versus serum dependent ) ; B ) the initial cell population ( Ficoll separated mononucleated cells versus CD 34 ( + ) selected cells ) , and C ) the low concentration of recombinant cytokines , flt 3 ligand , and thrombopoietin in association with a basic cocktail of stem cell factor , interleukin ( IL ) 6 , IL 3 , GM CSF , and erythropoietin . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We studied O 2 ( o ) production and p47phox phosphorylation in synovial fluid ( SF ) from patients with rheumatoid arthritis ( RA ) and spondylarthropathy ( SpA ) according to TNFalpha , IL 8 and GM CSF levels . ^^^ High levels of TNFalpha and IL 8 ( but not GM CSF ) are detected in patient ' s SF ( compared to circulating blood levels ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CD 34 progenitor cells were first amplified in vitro with stem cell factor ( SCF ) , Flt 3 ligand ( FL ) , thrombopoietin and IL 6 / soluble IL 6 receptor fusion protein , and cells were then induced to differentiate into DC with IL 4 and GM CSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In the current study , AML cell engraftment in standard NOD / SCID mice was compared to that obtained with NOD / SCID mice transgenic for the human growth factor genes Steel factor ( SF ) , interleukin 3 ( IL 3 ) and granulocyte macrophage colony stimulating factor ( GM CSF ) ( N / S S / GM / 3 ) as well as beta 2 microglobulin null NOD / SCID ( N / S beta 2m ( / ) ) mice . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Progenitor cells from the blood of normal and asthmatic subjects were cultured for 14 days in methylcellulose with GM CSF , stem cell factor , IL 3 and IL 5 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
To study the biological role of human cultured bone marrow mesenchymal stem cell ( BM MSC ) in hematopoiesis by investigation of its expression of multiple hematopoietic growth factors , RT PCR was used to analyze the expression of SCF , Flt 3 ligand , TPO , LIF , G CSF , GM CSF , IL 3 , IL 6 and IL 11 at mRNA level for human BM MSC from healthy donors and patients with leukemia and lymphoma . ^^^ The results clearly showed that the cultured BM MSC expressed mRNA of SCF , Flt 3 ligand , TPO , LIF , IL 6 and IL 11 at passages 3 up to 15 , but did not express G CSF , GM CSF and IL 3 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Therefore , in the present study , we have investigated in colorectal cancer patients the serum levels of selected HGFs , such as stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and M CSF in relation to controls and to the classical tumor markers , such as carcinoembryonic antigen ( CEA ) and carbohydrate antigen 19 9 ( CA 19 9 ) in colorectal cancer . ^^^ There were significant differences in the levels of circulating SCF , IL 3 , M CSF , GM CSF , and CEA and CA 19 9 in the colorectal cancer patients compared to the control group . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The multiple number of CFU GM increased from 2 . 3 + / 0 . 8 to 7 . 8 + / 1 . 9 times when Ang 2 was added into SCF + G CSF + GM CSF + IL 3 combination . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
GM CSF dependent human cell line MO7e was stimulated with GM CSF with SCF , and investigated activation of p70S6K by using phospho specific antibody . ^^^ A possible contribution of p70S6K to SCF synergy was examined by measuring p 21 induction as a model system . p70S6K was slightly activated by GM CSF alone and markedly activated by SCF alone . ^^^ These data suggest that synergistically activated p70S6K by GM CSF plus SCF involves , at least in part , protein translational control including regulation of p 21 protein . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
CD34+ cell derived hematopoietic precursors amplified with FLT 3 ligand , thrombopoietin and stem cell factor became , after a 6 day induction with GM CSF , IL 4 and TGF beta 1 , HLA DR+ , CD1a+ , CD 83 , CD 86 , CD 80 cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In 10 day suspension cultures of normal or CML CD34+ cells supplemented with G CSF , stem cell factor ( SCF ) , and granulocyte macrophage colony stimulating factor ( GM CSF ) , CML cells had diminished sensitivity to the growth inhibitory effect of elastase . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
SCF enhances IgE dependent release of mediators from mast cells , including histamine , leukotrienes , cytokines ( TNF alpha , IL 5 , GM CSF ) and chemokines ( RANTES / CCL5 , MCP 1 / CCL2 , TARC / CCL17 e MDC / CCL22 ) ; it is required for IL 4 production in mast cells . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In cultures with only lineage unspecific cytokines ( IL 3 , SCF , GM CSF ) , BAALC transcripts persisted up to day 20 , while CD 34 transcripts disappeared earlier . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Here , we show that nonobese diabetic severe combined immunodeficiency mice genetically engineered to produce ng / ml serum levels of human interleukin 3 ( IL 3 ) , granulocyte / macrophage stimulating factor ( GM CSF ) and Steel factor ( SF ) display a complex phenotype when transplanted with primitive human bone marrow ( BM ) or fetal liver cells . ^^^ Our findings suggest that long term exposure of primitive human hematopoietic cells to elevated levels of human IL 3 , GM CSF and SF in vivo may deleteriously affect the stem cell compartment , while expanding terminal myelopoiesis . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
This study demonstrates that PU . 1 ( / ) fetal erythroid progenitors are synergistically expanded by TPO plus SCF , but not combinations of EPO plus SCF , IL 3 or GM CSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Results of preclinical and clinical growth factor therapy studies with G CSF , GM CSF , pegylated G CSF , SCF , and IL 3 are summarized . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Dendritic cells were generated from cord blood CD34+ cells by culture with GM CSF , SCF , and TNF alpha , or from peripheral blood adherent cells or CD14+ cells by culture with GM CSF and IL 4 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
RESULTS : Proliferation by CFU GM and BFU E was enhanced synergistically when common beta chain receptor cytokines ( IL 3 or GM CSF ) were combined with G CSF or EPO , but not with gp 130 receptor cytokines ( LIF or IL 6 ) or tyrosine kinase receptor cytokines ( SCF , HGF , Flt 3 ligand , or PDGF ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
BUC ID suppressed the production of solubleVCAM 1 by RA bone marrow CD34+ cells stimulated with SCF , GM CSF and TNF alpha more effectively than BUC . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Finally , in vitro proliferation , but not differentiation , of CD34+ cells cultured in the presence of fms like tyrosine kinase 3 ligand ( FLT3L ) , stem cell factor , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 , and G CSF is profoundly inhibited by treatment with CsA in a dose dependent manner . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Different concentrations of CD 34 ( + ) cells were grown in gas permeable Teflon bags with different serum free and serum containing media supplemented with GM CSF , IL 4 , TNF alpha , SCF , Flt 3L and INF alpha . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In this study , the effect of hematopoietic cytokines , i . e . , granulocyte colony stimulating factor ( G CSF ) , stem cell factor ( SCF ) , and granulocyte macrophage colony stimulating factor ( GM CSF ) , on renal function was studied in cisplatin induced acute renal failure in mice . ^^^ There was no significant change observed among these parameters following treatment with SCF or with GM CSF . ^^^ In conclusion , G CSF , but not SCF or GM CSF , acts to accelerate regeneration and prevent apoptosis of renal tubular epithelial cells and leads to reduced renal injury in cisplatin induced acute renal failure in mice . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Evidence provided that the most efficient long term outgrowth of DC progenitors was obtained by GM CSF independent culture systems with the aid of Flt3 / Flk 2 and IL 6 , not with c kit ligand and IL 6 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , granulocyte colony stimulating factor ( G CSF ) and macrophage colony stimulating factor ( M CSF ) are members of a group of glycoproteins called hematopoietic cytokines ( HCs ) . ^^^ Stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , granulocyte colony stimulating factor ( G CSF ) and macrophage colony stimulating factor ( M CSF ) are members of a group of glycoproteins called hematopoietic cytokines ( HCs ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
HGF including SCF , FL , G CSF , GM CSF , M CSF and IL 6 were expressed in hPDAC . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Moreover , cell lines expressed interleukin ( IL ) 1 beta , IL 6 , granulocyte macrophage colony stimulating factor ( GM CSF ) , G CSF and stem cell factor ( SCF ) , and secreted variable amount of IL 1 beta , IL 6 and GM CSF . ^^^ However , the inductive capacity was significantly higher in the case of cryopreserved cells , where the rise in clonal growth reached that induced by the addition to the culture media of IL 3 , GM CSF and SCF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : A human osteoclastogenesis model , using as precursors colony forming unit granulocyte macrophage ( CFU GM ) colonies generated from umbilical cord mononuclear cells cultured in methylcellulose with GM CSF , interleukin ( IL ) 3 and stem cell factor ( SCF ) , has been developed . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Qualitatively , BB gelatin induced secretion of KC , MIP 2 , MCP 1 , RANTES , and MIP 1a as well as IL 6 but not 6Ckine , CTACK , Eotaxin , G CSF , GM CSF , IL 2 , 3 , 4 , 5 , 9 , 10 , 12 , 13 , 17 , Leptin , IFN gamma , SCF , sTNFri , TARC , TNF alpha , TIMP 1 , Tpo , and VEGF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
RESULTS : The optimal compositions of serum substitutes and the cytokine cocktail for HSC expansion in the MNC culture system were BIT ( 4 g / L BSA , 0 . 71 microg / mL insulin , and 27 . 81 microg / mL transferrin ) , and CC 9 ( 5 . 53 ng / mL TPO , 2 . 03 ng / mL IL 3 , 16 ng / mL SCF , 4 . 43 ng / mL FL , 2 . 36 ng / mL IL 6 , 1 . 91 ng / mL G CSF , 1 . 56 ng / mL GM CSF , 2 . 64 ng / mL SCGF , and 0 . 69 ng / mL IL 11 ) in the Iscove ' s modified Dulbecco ' s medium . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In addition , mitogenic cytokines SCF , IL 3 , and GM CSF induced colony formation of K 562 cells that could be inhibited by PD 98059 or enhanced by SB 203580 , respectively , indicating that these mitogenic cytokines induce K 562 colony formation by activating ERK and inactivating p 38 MAPK . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
By means of flow cytometry , CD34+ / CD38 hematopoietic stem cells ( HSC ) were collected from umbilical cord blood ( UCB ) of 10 healthy women at the time of delivery and cultivated in stem cell culture media supplemented with cell growth stimulating factors ( IL 3 , IL 6 , GM CSF , EPO , IGF 1 , and SCF ) for long periods . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Therefore , in the present study , we investigated in pancreatic cancer patients the serum levels of selected HCs , such as stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , granulocyte colony stimulating factor ( G CSF ) and macrophage colony stimulating factor ( M CSF ) in relation to a control group and to a group of patients with chronic pancreatitis . ^^^ There were significant differences in the levels of circulating SCF , IL 3 , GM CSF , M CSF , CEA and CA 19 9 in the pancreatic cancer patients compared to the control group . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
RESULTS : USSC constitutively produced SCF , LIF , TGF 1beta , M CSF , GM CSF , VEGF , IL 1beta , IL 6 , IL 8 , IL 11 , IL 12 , IL 15 , SDF 1alpha , and HGF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The purpose of this study was to investigate the radioprotective effect of HGFs ( GM CSF , IL 3 and SCF ) in irradiated human peripheral blood mononuclear cells ( PBMCs ) in vitro , and the survival effect of lethally irradiated C3H mice in vivo . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In PSBS SCM , the levels of IL 6 , IL 17 , G CSF and GM CSF were markedly increased , but not those of IL 3 and SCF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Cytokine and growth factor genes , including IL 6 , IL 8 , IL 11 , IL 12 , IL 14 , IL 15 , LIF , G CSF , GM CSF , M SCF , FL and SCF , were found to be expressed in the MSCs . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The effects of cytokines such as TPO , GM CSF and SCF and of a low dose of H 202 on the transport activity and its kinetic parameters are compared . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Proliferation assay using a variety of cytokines demonstrated that the HSM 911 cells proliferate in response to recombinant granulocyte macrophage colony stimulating factor ( rGM CSF ) , recombinant Interleukin 3 ( rIL 3 ) and recombinant stem cell factor ( rSCF ) , but do not in response to recombinant granulocyte colony stimulating factor ( rG CSF ) , natural macrophage colony stimulating factor ( M CSF ) , rIL 1 , rIL 2 , rIL 4 , rIL 5 , rIL 6 or recombinant erythropoietin ( rEpo ) . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
After surgically excising established LLC tumors , mice received at the excision site gelatin sponge implants containing VEGF to chemoattract CD 34 ( + ) cells , and / or GM CSF plus SCF to induce CD 34 ( + ) cell differentiation into dendritic cells . ^^^ Implants containing VEGF , GM CSF / SCF or VEGF / GM CSF / SCF had a higher proportion of CD 34 ( + ) cells compared to control implants . ^^^ However , the number of dendritic cells was higher in implants containing GM CSF / SCF or VEGF / GM CSF / SCF than those containing either VEGF or diluent . ^^^ Regional lymph node from mice containing GM CSF / SCF or VEGF / GM CSF / SCF implants showed increased dendritic cell levels . ^^^ However , when lysates from LLC ( GFP ) were added to the implants , the highest proportion of dendritic cells associated with GFP was in lymph nodes of mice containing GM CSF / SCF implants . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Expansion of umbilical cord blood mononuclear cells ( UCB MNCs ) was carried out in a rotating wall vessel ( RWV ) bioreactor and tissue culture flasks ( T flasks ) in serum containing medium supplemented with relatively low doses of purified recombinant human cytokines ( 5 . 33 ng / ml IL 3 , 16 ng / ml SCF , 3 . 33 ng / ml G CSF , 2 . 13 ng / ml GM CSF , 7 . 47 ng / ml FL and 7 . 47 ng / ml TPO ) for 8 days . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
METHODS : CD34+ cells were seeded at 1 10 10 ( 5 ) / mL and expanded for 14 days in RPMI + 10 % autologous plasma supplemented with GM CSF , IL 4 , Flt 3L and SCF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Tryptase also induced the expression of mRNA for GM CSF and SCF , two cytokines known to promote growth of AML cells , in cultured bone marrow fibroblasts . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Lyn is renowned for its role in B cell antigen receptor and FcepsilonRI signaling ; however , it is becoming increasingly apparent that Lyn also functions in signal transduction from growth factor receptors including the receptors for GM CSF , IL 3 , IL 5 , SCF , erythropoietin , CSF 1 , G CSF , thrombopoietin and Flt 3 ligand . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In cultures of TRL 01 , addition of GM CSF , SCF , and G CSF and adhesion to fibronectin coated plates promoted transient proliferation and survival , although they did not support long term culture . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Response to the cytokines SCF , IL 3 , and GM CSF was determined by this assay for the leukemic mouse strain BXH 2 and ten other inbred mouse strains . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
We have generated immunodeficient scid / scid ( SCID ) transgenic mice expressing the genes for human interleukin 3 , granulocyte / macrophage colony stimulating factor , and stem cell factor . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
IL 4 , but not SCF , enhanced ionomycin induced transcription and secretion of several genes , including the cytokines IL 3 , IL 4 , granulocyte / macrophage colony stimulating factor , IL 8 and the receptor for IL 6 in the human HMC 1 mast cell line . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Recombinant stem cell factor , recombinant granulocyte macrophage colony stimulating factor , and catalase were also analyzed by TGGE , indicating that the technique can be used to analyze denaturation of monomeric and multimeric proteins . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
The priming effect on epinephrine induced platelet aggregation in hirudin PRP was unique to thrombopoietin , with no effects seen using interleukin 6 ( IL 6 ) , IL 11 , IL 3 , erythropoietin , granulocyte colony stimulating factor , granulocyte macrophage colony stimulating factor , or c kit ligand . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
IL 4 , stem cell factor ( SCF ) , tumor necrosis factor ( TNF ) , and granulocyte or granulocyte macrophage colony stimulating factor . hCG concentration was determined by immunoradiometric methods and cell number was enumerated by means of an electronic particle counter . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Furthermore , this intracellular marker of priming is selective for IL 5 related cytokines , in that it is observed following exposure to IL 5 and granulocyte macrophage colony stimulating factor but not to interferon gamma , stem cell factor , tumor necrosis factor alpha , or IL 4 . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
METHODS : Immunodeficient transgenic mice were developed by crossing transgenic founders carrying porcine interleukin 3 , granulocyte macrophage colony stimulating factor , and stem cell factor genes with severe combined immunodeficient mice or non obese diabetic / severe combined immunodeficient mice . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
JMML arises from clonal expansion of a hematopoietic stem cell , and JMML cells and murine Nf 1 / hematopoietic cells are hypersensitive to granulocyte macrophage colony stimulating factor and KitL , the ligand for c kit . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
PERV was released from cultured porcine bone marrow cells without stimulation , and combinations of the porcine hematopoietic cytokines , interleukin 3 , granulocyte macrophage colony stimulating factor and stem cell factor had no additional effect on the infectivity or in vitro tropism of released PERV virions . . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
In vitro generation of DC was achieved using a cytokine cocktail containing tumor necrosis factor alpha , granulocyte macrophage colony stimulating factor , c kit ligand , and soluble CD 40 ligand ; yet , addition of the HDAC inhibitor ( Hdi ) trichostatin A enhanced DC differentiation with retention of the fusion transcripts . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Growth of RA A progenitors in cytokines promoting myelopoiesis ( GMCSF , TNF , stem cell factor ) produced increased monocyte and dendritic cell progeny , in support of the flow cytometry data . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Several other factors have a direct ( interleukin 3 , 6 , 11 , 13 , GMCSF , erythropoietin ) or indirect ( interleukin 1 , stem cell factor ) positive effect . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
To study this , we incubated peripheral blood lymphocytes from CLL patients with the following cytokines : EPO , SCF , TNFalpha , TGFbeta , GMCSF , TPO , IL 1 , IL 2 , IL 3 , IL 4 , GCSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
Eighteen AML samples were cultured for 7 d in serum free medium with or without a supplement of recombinant cytokines comprising c kit ligand , IL 3 and GMCSF . ^^^
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P21583	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.68413123
Stem cell factor ( SCF , also known as mast cell growth factor , Steel factor or Kit ligand ) has been found to synergize with GM CSF , IL 6 , IL 3 , IL 11 or Epo to increase the numbers of megakaryocyte containing colonies ( i . e . , CFU Meg , BFU Meg , CFU GMM , CFU GEMM ) . 0.68413123^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.54127254
Mast cell growth factor ( MGF , the ligand for c kit receptor ) can stimulate proliferation of factor dependent myeloid cell line , M07e , and MGF synergizes with granulocyte macrophage colony stimulating factor ( GM CSF ) or IL 3 in this effect . 0.54127254^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.62043646
The interactions of SCF with GM CSF , TNF , interleukin 3 ( IL 3 ) and macrophage colony stimulating factor ( M CSF ) in promoting CFU DL development have now been studied in serum free cultures of unfractionated as well as progenitor enriched cells from either bone marrow or cord blood . 0.62043646^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.50742464
The effects on hematopoiesis of recombinant stem cell factor ( ligand for c kit ) administered in vivo to mice either alone or in combination with granulocyte colony stimulating factor . 0.50742464^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.55760218
Recombinant rat stem cell factor synergizes with recombinant human granulocyte colony stimulating factor in vivo in mice to mobilize peripheral blood progenitor cells that have enhanced repopulating potential . 0.55760218^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.86985898
Steel factor ( SF ) , the ligand for the proto oncogene c kit , acts synergistically with GM CSF or IL 3 to support the growth of normal human hematopoietic progenitor cells . 0.86985898^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Human mast cell growth factor ( MGF , a c kit ligand ) and colony stimulating factors ( Epo , GM CSF , G CSF , IL 3 ) were assessed in the absence or presence of serum for stimulation in semi solid medium of single CD 34 , CD 34 HLA DR+ , or CD 34 HLA DR+CD33 cells sorted per microtiter well . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The present studies analyzing stromal cytokine messenger RNA ( mRNA ) production by standard Northern blot analysis show constitutive production of mRNAs for CSF 1 , GM CSF , granulocyte CSF ( G CSF ) , c kit ligand ( KL ) , and interleukin 6 ( IL 6 ) , but not IL 3 , IL 4 , or IL 5 by 3 week irradiated or nonirradiated murine Dexter stromal cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We tested the ability of recombinant human ( rhu ) mast cell growth factor ( MGF ) , also known as c kit ligand , to stimulate the colony formation of human bone marrow cells in semisolid medium alone and in combination with rhu erythropoietin ( EPO ) , rhu Interleukin 3 ( IL 3 ) , rhu granulocyte colony stimulating factor ( G CSF ) and rhu granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Purified natural ( n ) and recombinant ( r ) murine ( mu ) mast cell growth factor ( MGF , a c kit ligand ) were evaluated alone and in combination with r human ( hu ) erythropoietin ( Epo ) , rhu granulocyte macrophage colony stimulating factor ( rhuGM CSF ) , rhuG CSF , and / or rhuM CSF for effects in vitro on colony formation by multipotential ( colony forming unit granulocyte , erythroid , monocyte , megakaryocyte [ CFU GEMM ] ) , erythroid ( burst forming unit erythroid [ BFU E ] ) and granulocyte macrophage ( CFU GM ) progenitor cells from normal human bone marrow . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CD 45 cell surface antigens are linked to stimulation of early human myeloid progenitor cells by interleukin 3 ( IL 3 ) , granulocyte / macrophage colony stimulating factor ( GM CSF ) , a GM CSF / IL 3 fusion protein , and mast cell growth factor ( a c kit ligand ) . ^^^ A possible link was evaluated between expression of CD 45 antigens on human myeloid progenitor cells ( MPC ) ( colony forming unit granulocyte / macrophage [ CFU GM ] , burst forming unit erythroid [ BFU E ] , and colony forming unit granulocyte / erythroid / macrophage / megakaryocyte [ CFU GEMM ] ) and regulation of MPC by colony stimulating factors ( CSF ) ( interleukin 3 [ IL 3 ] , GM CSF , G CSF , M CSF , and erythropoietin [ Epo ] ) , a GM CSF / IL 3 fusion protein , and mast cell growth factor ( MGF ; a c kit ligand ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Murine mast cell growth factor ( muMGF ) , a c kit ligand , has additive to greater than additive effects on in vitro colony formation of murine and human myeloid progenitor cells stimulated with erythropoietin , granulocyte macrophage colony stimulating factor ( GM CSF ) , and / or interleukin ( IL ) 3 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
An evaluation of the effects of a recombinant , soluble form of the c kit ligand alone and in combination with either granulocyte macrophage colony stimulating factor ( GM CSF ) or interleukin 3 ( IL 3 ) on the regulation of human megakaryocytopoiesis was performed using a serum depleted clonal assay system and a long term bone marrow culture system . ^^^ The c kit ligand alone had no megakaryocyte colony stimulating activity ( MK CSA ) but was capable of augmenting the MK CSA of both GM CSF and IL 3 . ^^^ In the case of the BFU MK , the c kit ligand synergistically augmented the numbers of colonies formed in the presence of IL 3 , but not GM CSF , but increased the size of BFU MK derived colonies cloned in the presence of both of these cytokines . ^^^ However , at the level of the CFU MK , c kit ligand synergized with both GM CSF and IL 3 by increasing both colony numbers and size . ^^^ Although the c kit ligand alone exhibited limited potential in sustaining long term megakaryocytopoiesis in vitro , it synergistically augmented the ability of IL 3 , but not GM CSF , to promote long term megakaryocytopoiesis . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Cells were cultured in suspension in the presence of c kit ligand , interleukin 3 , interleukin 6 , erythropoietin , and granulocyte colony stimulating factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Colony stimulating activity was provided by the recombinant human colony stimulating factors granulocyte macrophage colony stimulating factor ( GM CSF ) , granulocyte colony stimulating factor ( G CSF ) , interleukin 3 ( IL 3 ) , fusion protein ( FP ) , c kit ligand ( KL ) or GM CSF combined with other cytokines ( KL , IL 3 ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
To determine if the c kit ligand mast cell growth factor ( MGF ) can improve progenitor growth in MDS , we evaluated in vitro responsiveness of bone marrow progenitors from 25 patients to MGF and / or GM CSF , interleukin 3 ( IL 3 ) and PIXY 321 , and examined the relationship between progenitor response and cellular expression of the c kit receptor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The aim of this study was to analyze the potential of c kit ligand ( CKL ) combined with granulocyte macrophage colony stimulating factor ( GM CSF ) and / or interleukin 3 ( IL 3 ) to induce proliferation and differentiation of human bone marrow CD34+ cells in vitro . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The effects of recombinant canine granulocyte colony stimulating factor ( rcG CSF ) and recombinant canine stem cell factor ( rcSCF ) , a c kit ligand , on the circulation of hematopoietic progenitor and stem cells were studied in a canine model . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We have studied the effects of recombinant human interleukin 9 ( IL 9 ) , alone and combined with stem cell factor ( SCF , c kit ligand ) , IL 3 , and granulocyte macrophage colony stimulating factor ( GM CSF ) on the clonogenic proliferation of highly enriched human hematopoietic CD34+ and CD34+CD33 DR progenitor cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Mast cell growth factor ( C kit ligand ) in combination with granulocyte macrophage colony stimulating factor and interleukin 3 : in vivo hemopoietic effects in irradiated mice compared to in vitro effects . ^^^ In the presence of hemopoietic cytokines such as granulocyte macrophage colony stimulating factor ( GM CSF ) and interleukin 3 ( IL 3 ) , mast cell growth factor ( MGF ; also known as steel factor , stem cell factor , and c kit ligand ) has proven to be a potent hemopoietic regulator in vitro . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Pretreatment with epoxybutene in vitro suppressed recombinant interleukin 3 stimulated colony formation in haematopoietic stem and progenitor cells , had no effect on colony formation with recombinant granulocyte / macrophage colony stimulating factor or recombinant granulocyte colony stimulating factor alone , and completely blocked the synergism of recombinant c kit ligand and granulocyte / macrophage colony stimulating factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
A study of gene expression of GM CSF , IL 3 , c kit ligand , and IL 1 by bone marrow stromal cells of mice who were treated with an LD 50 cytosine arabinoside ( Ara C ) dose was carried out . ^^^ We conclude that GM CSF , c kit ligand and IL 1 have important roles in sustaining marrow regeneration following extensive damage . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Colony formation was assessed in 1 degree wells containing various cytokines , alone and in combination , and single colonies deriving after 21 days in semisolid medium were replated into 2 degree wells in the presence of the combination of purified preparations of recombinant human steel factor ( SF , a c kit ligand ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , granulocyte colony stimulating factor ( G CSF ) , interleukin 3 ( IL 3 ) , and erythropoietin ( Epo ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Stem cell factor ( CSF ) , also called c kit ligand ( KL ) or mast cell growth factor ( MGF ) is a peptide growth factor / cytokine with broad activities , especially on hematopoiesis . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , a c kit ligand , has a preferential effect on the proliferation of several classes of immature hematopoietic progenitor cells in combination with GM CSF or IL 3 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Expansion of immunostimulatory dendritic cells among the myeloid progeny of human CD34+ bone marrow precursors cultured with c kit ligand , granulocyte macrophage colony stimulating factor , and TNF alpha . ^^^ These bone marrow CD34+ derived dendritic cell that develop during 2 wk culture have the same morphologic , phenotypic , and functional properties that distinguish mature dendritic cells in blood . c kit ligand does not directly influence dendritic cell differentiation per se , but rather increases the total cell number in synergistic combination with GM CSF and TNF alpha . ^^^ The synergistic combination of c kit ligand , GM CSF , and TNF alpha therefore expands substantial numbers of immunostimulatory CD 14 HLA DR+ dendritic cells from defined CD34+ progenitors in human bone marrow . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
SPY 3 2 expressed high levels of c kit ligand and low levels of granulocyte macrophage colony stimulating factor and interleukin 3 , and these molecules were all involved in this long term erythropoiesis . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Effects of recombinant canine stem cell factor , a c kit ligand , and recombinant granulocyte colony stimulating factor on hematopoietic recovery after otherwise lethal total body irradiation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The ability of CD34+ / c kit+ cells to form multilineage colonies ( CFU GEMM ) was assayed after sorting with an antibody that did not show any significant effect on c kit ligand ( RL ) or granulocyte / macrophage colony stimulating factor ( GM CSF ) induced colony formation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In clonogenetic dishes , graded amounts of c kit ligand ( KL ) were added together with saturating levels of erythropoietin ( Ep ) and variable amounts of interleukin 3 and granulocyte macrophage colony stimulating factor ( IL 3 / GM CSF ) , ie , high or low level , or no IL 3 / GM CSF addition . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
To that effect , stromal cell free LTBMC were initiated with CD34+ HLA DR CD 15 rhodamine 123dull bone marrow cells and were maintained with repeated addition of c kit ligand and a synthetic interleukin 3 / granulocyte macrophage colony stimulating factor fusion protein . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Four cytokine combinations were tested : ( 1 ) mast cell growth factor ( MGF ; a c kit ligand ) and interleukin 6 ( IL 6 ) ; ( 2 ) MGF , IL 6 , IL 3 , and erythropoietin ( Epo ) ; ( 3 ) MGF , IL 6 , granulocyte macrophage colony stimulating factor ( GM CSF ) / IL 3 fusion protein ( FP ) , macrophage colony stimulating factor ( M CSF ) , and granulocyte CSF ( G CSF ) ; and ( 4 ) MGF , IL 6 , FP , M CSF , G CSF , and Epo . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CD34+ cells enriched from bone marrow were cultured for up to 5 weeks in interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) and granulocyte colony stimulating factor ( G CSF ) with or without stem cell factor ( SCF ) ( also termed c kit ligand ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In both murine and human systems the c kit ligand , also known as mast cell growth factor ( MGF ) , acts synergistically with several colony stimulating factors , including the granulocyte macrophage colony stimulating factor ( GM CSF ) and interleukin 3 ( IL 3 ) , in stimulating the proliferation and differentiation of different types of hematopoietic progenitors . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Effect of c kit ligand ( stem cell factor ) in combination with interleukin 5 , granulocyte macrophage colony stimulating factor , and interleukin 3 , on eosinophil lineage . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Effect of stem cell factor ( c kit ligand ) , granulocyte macrophage colony stimulating factor and interleukin 3 on hematopoietic progenitors in human long term bone marrow cultures . ^^^ We evaluate the effects of recombinant human stem cell factor ( SCF or c kit ligand ) alone and also in combination with recombinant human granulocyte macrophage colony stimulating factor ( GM CSF ) and interleukin 3 ( IL 3 ) , on cell proliferation and differentiation in human long term bone marrow cultures ( LTBMC ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Cell line stimulation and antibody blocking indicated the presence of c kit ligand , interleukin 6 ( IL 6 ) and granulocyte colony stimulating factor ( G CSF ) . ^^^ Baseline or `` constitutive ' ' expression of BMM and mRNA for CSF 1 and c kit ligand was seen . ^^^ There was a biphasic response of c kit ligand mRNA expression to CSF 1 exposure ; low levels of CSF 1 ( 50 U / mL ) induced , while higher levels ( 2000 U / mL ) inhibited , expression . ^^^ These data indicate that BMM ( and by analogy the macrophage component of Dexter culture stroma ) , are important sources of CSF 1 and c kit ligand but not GM CSF or IL 3 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Human stem cell factor ( c kit ligand ) induces an autocrine loop of growth in a GM CSF dependent megakaryocytic leukemia cell line . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Particularly the identification of new surface membrane molecules such as CD 40 ligand on basophils or c kit on mast cells , and of new triggering agents and modulators of mediator release such as IL 3 , IL 5 , GM CSF and nerve growth factor ( for basophils ) or c kit ligand ( for mast cells ) allows a better understanding of the regulation of these cell types . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
HSNC clones produced various cytokines , including IL 6 , IL 7 , IL 8 , IL 9 , granulocyte CSF ( G CSF ) , granulocyte macrophage CSF ( GM CSF ) , macrophage CSF ( CSF 1 ) , TGF beta 1 , and c kit ligand , but could not produce IL 1 alpha , IL 1 beta , IL 2 , IL 3 , IL 4 , TNF alpha , or TNF beta . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
These results indicate that CPA is a novel factor distinct from IL 1 , IL 3 , G , M , GM CSF , IL 6 and SCF ( c kit ligand ) . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In clonogenetic fetal calf serum supplemented ( FCS+ ) or nonsupplemented ( FCS ) culture treated with saturating levels of interleukin 3 ( IL 3 ) granulocyte macrophage colony stimulating factor ( GM CSF ) and erythropoietin ( Ep ) ( combined with c kit ligand in FCS ( ) culture conditions ) , RA induces a dramatic dose dependent shift from erythroid to granulomonocytic colony formation , the latter colonies being essentially represented by granulocytic clones . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Methylcellulose committed progenitor cell assays were carried out using FL alone and in combinations with granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) and c kit ligand ( KL ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
To further address the role of IL 9 in the development of acute leukemia , we evaluated the proliferative response of three leukemic cell lines and 32 primary samples from acute myeloblastic leukemia ( AML ) patients to recombinant human ( rh ) IL 9 alone and combined with rh IL 3 , granulocyte macrophage CSF ( GM CSF ) , and stem cell factor ( [ SCF ] c kit ligand ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We have studied the effects of recombinant human interleukin 11 ( rhIL 11 ) , alone and combined with stem cell factor ( SCF or c kit ligand ) , IL 3 , and granulocyte macrophage colony stimulating factor ( GM CSF ) on the proliferation of highly enriched human hematopoietic CD34+ and CD34+CD33 DR progenitor cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Low numbers of day 9 EB cells ( 5 10 10 ( 5 ) or less ) cultured in the presence of several growth factors ( interleukin 3 [ IL 3 ] , IL 1 , c kit ligand , basic fibroblast growth factor , insulin growth factor 1 , IL 6 , granulocyte colony stimulating factor , fetal liver kinase 2 ligand ) develop few or no CFCs after 1 week of culture . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Expansion of marrow in all cases was accomplished through the use of added cytokines such as IL 3 , GM CSF , and c kit ligand . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The priming effect on epinephrine induced platelet aggregation in hirudin PRP was unique to thrombopoietin , with no effects seen using interleukin 6 ( IL 6 ) , IL 11 , IL 3 , erythropoietin , granulocyte colony stimulating factor , granulocyte macrophage colony stimulating factor , or c kit ligand . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Using a plasma clot clonal assay and a long term bone marrow culture ( LTBMC ) system , we studied the effects of the recently cloned human flt 3 ligand ( FL ) alone and in combination with granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) , or stem cell factor ( c kit ligand [ KL ] ) on human megakaryocytopoiesis . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In contrast , cytokines such as IL 1 , IL 2 , IL 3 , IL 4 , IL 6 , G CSF , GM CSF , or SCF ( c kit ligand ) altered neither the proliferation capacity nor the phenotypical constellation of LW / SO cells ( each tested alone ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We also used the progenitor assay to screen several human and murine recombinant cytokines for cross reactivity to swine bone marrow cells , including interleukin 3 ( IL 3 ) , IL 6 , Il 11 , granulocyte and granulocyte macrophage colony stimulating factors ( G CSF and GM CSF ) , c kit ligand ( also called mast cell growth factor [ MGF ] ) , and erythropoietin ( Epo ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Isolated CD34+lin+ cells formed increased numbers of colony forming cells ( CFC ) when flt 3l was added together with IL 3 , IL 6 , G CSF , GM CSF or c kit ligand ( KL ) , or with the combination of IL 3 and KL . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Continuous culture of these hematopoietic cells in the presence of the cytokines interleukin 3 , c kit ligand , granulocyte colony stimulating factor resulted in tenfold expansion by day 7 and 25 fold expansion by day 14 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CD34+ precursors in normal human bone marrow ( BM ) generate large numbers of dendritic cells alongside macrophages and granulocytic precursors when cultured for 12 to 14 days in c kit ligand , granulocyte macrophage colony stimulating factor ( GM CSF ) , and tumor necrosis factor alpha ( TNF alpha ) . ^^^ Therefore , normal CD34+ BM precursors can generate a post CFU bipotential intermediate in the presence of c kit ligand , GM CSF , and TNF alpha . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
When selected PB CD34+ cells that were expanded ex vivo with combinations of human hematopoietic growth factors including the c kit ligand ( KL ) , interleukin ( IL ) 1 beta , IL 3 , IL 6 , erythropoietin ( EPO ) and granulocyte macrophage colony stimulating factor ( GM CSF ) for 14 days were grafted to cotransplant carrying SCID mice , a considerable loss of their proliferative potential was observed regardless of the HGF combination used . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We have investigated the mobilization of these cells into the blood of patients with ovarian carcinoma randomized to receive granulocyte colony stimulating factor ( G CSF ; 5 micrograms / kg ) plus different doses of stem cell factor ( SCF ; c kit ligand ) after chemotherapy or G CSF alone after chemotherapy . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We have performed a comprehensive analysis of cell lines and tissues to compare and contrast the expression patterns of Flt 3 ligand ( FL ) , c Kit ligand ( KL ) , and macrophage colony stimulating factor as well as their receptors , Flt 3 , c Kit , and c Fms . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In quiescent HPCs the receptors for early acting [ flt 3 ligand ( FL ) , c kit ligand ( KL ) , bFGF , interleukin 6 ( IL 6 ) ] and multilineage [ IL 3 , granulocyte macrophage CSF ( GM CSF ) ] HGFs are expressed at significant levels but with different patterns , eg , kit and flt 3 are detected on a majority and minority of HPCs , respectively , whereas IL 3Rs and GM CSFRs are present on almost all HPCs . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In contrast , in mice treated with either GM CSF , GM CSF plus IL 4 , c kit ligand ( c kitL ) , or G CSF , class II+ CD11c+ cells were not significantly increased . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In this study , the effects of c kit ligand ( KL ) and flt 3 ligand ( FL ) were compared in the presence of IL 3 , GM CSF , and erythropoietin ( 3 / GM / EPO ) , using frequent medium exchange cultures of human bone marrow mononuclear cells ( BMMNC ) and CD 34 enriched cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We observed no significant alterations in bone marrow stromal cell proliferation or the production of IL 6 , GM CSF , soluble c kit ligand and TNF alpha following HCMV replication in either stimulated lipopolysaccharide ( LPS ) or unstimulated conditions . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Indeed , it has been shown that tyrosine kinase dependent stimulation of CD34+ hematopoietic cell lines with c kit ligand ( KL ) , IL 3 or GM CSF transiently activates the ability of VLA 4 to bind to VCAM 1 or FN . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
MGDF also enhanced interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , c kit ligand ( KL ) and FLT 3 ligand ( FL ) stimulated blast cell proliferation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The RT PCR investigation evidences the mRNA encoding for bFGF , IL 7 , GM CSF , and SCF ( c kit ligand ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The proliferative effects of SP correlate with the induction of positive hematopoietic growth factors such as IL 3 , IL 6 , GM CSF and c kit ligand and the inhibitory effects by NK A correlate with the induction of two negative hematopoietic regulators , MIP 1 alpha and TGF beta . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Selected CD34+ cells from mobilized apheresis products were cultured in serum free or serum containing media supplemented with granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) , and stem cell factor ( SCF ; c kit ligand ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Functionally distinct apoptotic schedules were associated with different phases of DC development when multipotent CD34+ progenitor cells were treated with GM CSF + TNF + / SCF ( c kit ligand ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We first evaluated the proliferative response of 3 leukemic cell lines and 32 primary samples from AML patients to IL 9 alone and combined with rh IL 3 , granulocyte macrophage colony stimulating factor ( GM CSF ) and stem cell factor ( SCF , c kit ligand ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Culturing CD34+ cells with stem cell factor ( c kit ligand ) plus granulocyte macrophage colony stimulating factor resulted in the appearance of a significant proportion of cells expressing phenotypic markers characteristic of dendritic cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Blasts were cultured for 2 weeks in a combination of cytokines c Kit ligand , interleukin 3 and granulocyte macrophage colony stimulating factor ( SIGm mix ) together with all trans retinoic acid or 1alpha , 25 dihydroxy vitamin D 3 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CD34+ cells purified from leukapheresis product were seeded at 1 10 10 ( 4 ) cells / mL in complete medium supplemented with GM CSF , TNF alpha , IL 4 , c kit ligand , and flt 3 ligand ( FL ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
By adding TPO to all possible combinations of GM CSF , IL 3 , and c kit ligand ( CKL ) in a suspension culture system , we found a significant increase in both relative and absolute numbers of cells in cultures containing TPO of the megakaryocytic lineage and CD34+ cells after 14 days of culture . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Despite this low level expression , flt 3 L exerted synergistic effects with a combination of c kit ligand , IL 3 , IL 6 , GM CSF and G CSF , mainly to induce proliferation of CD34+ / CD38 cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Selected progenitors were then cultured in the mixed colony assay with incremental concentrations of combinations containing erythropoietin ( Epo ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) and c kit ligand . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The combination of cytokines flt 3 ligand + c kit ligand + granulocyte macrophage colony stimulating factor ( GM CSF ) + interleukin 4 ( IL 4 ) + tumor necrosis factor alpha ( TNF alpha ) was used to maximize the output of mature DCs in the culture of CD34+ cells while minimizing the production of monocytes . ^^^ Dendritic cells generated from CD34+ progenitor cells with flt 3 ligand , c kit ligand , GM CSF , IL 4 , and TNF alpha are functional antigen presenting cells resembling mature monocyte derived dendritic cells . ^^^ Differentiation was marked by a time dependent increase in allostimulatory function , which , at its peak , was more potent than in cultures of DCs generated from monocytes with GM CSF + IL 4 , but was comparable on a cell to cell basis to that of mature monocytes cultured in flt 3 ligand + c kit ligand + GM CSF + IL 4 + TNF alpha . ^^^ Altogether , these results suggest that DCs generated from CD34+ cells of patients with breast cancer with flt 3 ligand , c kit ligand , GM CSF , IL 4 , and TNF alpha are competent antigen presenting cells , particularly for CD8+ cytotoxic T lymphocytes , and resemble mature monocyte derived DCs in the assays described here . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Also in CD34+ precursors , cultured in the presence of flt 3 ligand , c Kit ligand , GM CSF , IL 4 and TNF alpha , addition of anti CD 33 mAb prevented the recovery of mature dendritic cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Eighteen AML samples were cultured for 7 d in serum free medium with or without a supplement of recombinant cytokines comprising c kit ligand , IL 3 and GMCSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
TGF beta , GM CSF , c kit ligand , and IL 7 are produced constitutively and IL 1beta , IL 6 , IL 8 , TNF alpha , IFN gamma , and MIP 1 alpha are upregulated upon stimulation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
HbF was moderately enhanced by interleukin 3 ( IL 3 ) and granulocyte macrophage colony stimulating factor treatment ( up to 5 % 8 % gamma globin content ) , while sharply reactivated in a dose dependent fashion by c kit ligand ( KL ) and NaB ( 20 % 23 % ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Among various combinations , the combination of c kit ligand , flt 3 ligand , c mpl ligand ( TPO ) , and interleukin ( IL ) 4 most potently generated the number of CD1a+CD14 DCs in cultures containing granulocyte macrophage colony stimulating factor ( GM CSF ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^ These findings demonstrated that the combination of c kit ligand plus flt 3 ligand plus TPO with GM CSF plus TNF alpha , followed by IL 4 , is useful for ex vivo generation of human DCs from mobilized CD34+ peripheral blood progenitors . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Immortalized human bone marrow endothelial cells ( BMEC ) transfected with an expression vector carrying cDNA encoding the human telomerase reverse transcriptase ( hTERT ) and human umbilical vein endothelial cells ( HUVEC ) were transfected with combinations of adenovectors expressing murine c kit ligand ( KL ) , human thrombopoietin ( TPO ) , human Flt 3 ligand ( FL ) , and human granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Although mRNA for IL 3 and GM CSF was never detected , transcripts for c kit ligand and , more commonly , its receptor were . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
This study investigated early ex vivo expansion kinetics of UCB AC133+ cells ( 2 4 10 10 ( 4 ) / ml ) , mononuclear cells ( MNC , 1 2 10 10 ( 6 ) / ml ) and AC133negative cells ( AC 133 ( neg ) , 2 4 10 10 ( 4 ) / ml ) in stroma free 8 d liquid culture ( fetal bovine serum supplemented Iscove ' s modified Dulbecco ' s medium ( IMDM ) with either ' K36EG ' [ c Kit ligand , interleukin 3 ( IL 3 ) , IL 6 , erythropoietin , granulocyte colony stimulating factor ] or ' TPOFL ' ( thrombopoietin , Flt 3 ligand ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The adherent cells were differentiated into DCs within 2 weeks by adding granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 4 ( IL 4 ) , c kit ligand and tumour necrosis factor alpha ( TNF alpha ) . ^^^ CONCLUSIONS : Our findings indicate that plastic adherent peripheral blood cells , when cultured with GM CSF , IL 4 , c kit ligand and TNF alpha in autologous human plasma supplemented media , are a potent source of functional DCS that may be of value for human therapy . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In vitro generation of DC was achieved using a cytokine cocktail containing tumor necrosis factor alpha , granulocyte macrophage colony stimulating factor , c kit ligand , and soluble CD 40 ligand ; yet , addition of the HDAC inhibitor ( Hdi ) trichostatin A enhanced DC differentiation with retention of the fusion transcripts . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Evidence provided that the most efficient long term outgrowth of DC progenitors was obtained by GM CSF independent culture systems with the aid of Flt3 / Flk 2 and IL 6 , not with c kit ligand and IL 6 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
To evaluate the OP 9 capability to sustain long term hematopoiesis , we reported the expansion of granulocyte colony stimulating factor ( G CSF ) mobilized human peripheral blood ( PB ) CD 34 ( + ) cells in co culture with murine OP 9 and MS 5 stromal cells , either transfected with various combinations of adenovectors ( Ad ) expressing c kit ligand ( KL ) ( either soluble or transmembrane form ) , thrombopoietin ( TPO ) , flt 3 / flk2 ligand ( FL ) , and granulocyte macrophage ( GM ) CSF or with weekly addition of these cytokines . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In this report we investigated the growth of HPP CFC stimulated by combinations of interleukin ( IL ) 1 , IL 6 , kit ligand ( KL ) , granulocyte ( G ) colony stimulating factor ( CSF ) , macrophage CSF ( M CSF ) , granulocyte macrophage CSF ( GM CSF ) and IL 3 in clonal cultures incubated at 7 % or 21 % O 2 tension . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Neither granulocyte macrophage colony stimulating factor , interleukin 3 , interleukin 6 , nor the kit ligand induced the phosphorylation of the M ( r ) 75 , 000 receptor protein , although these growth factors induced the phosphorylation of other proteins . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
AIC2A and AIC2B transcription was transiently induced in these cells in response to a variety of hematopoietic growth factors , including erythropoietin ( EPO ) , monocyte CSF , IL 3 , GM CSF , and stem cell factor ( SCF or kit ligand ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The growth factor receptor phenotype of this immature cell fraction has been determined for granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) , and IL 6 as well as for kit ligand , making c kit an especially strong growth factor receptor marker for reconstituting stem cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
These include kit ligand ( KL ) , interleukins ( IL ) 1 , 3 and 6 and granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In addition , rapamycin blocks the proliferative response of cell lines to a variety of hematopoietic growth factors , including interleukin 3 ( IL 3 ) , interleukin 6 ( IL 6 ) , granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and kit ligand ( KL ) , suggesting that it should be a strong inhibitor of hematopoiesis . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Synergism between FL and either granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) or KIT ligand ( KL ) was observed in promoting the growth of high proliferative potential ( HPP ) colony forming cells ( CF ) and / or low proliferative potential ( LPP ) CFC in cultures of CD34++ CD38+ Lin and CD34++ CD 38 Lin LDFL cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The proliferation kinetics and clonogenic activity of CD34+ / 38hi ( CD38hi ) and CD34+ / 38lo ( CD38lo ) human marrow cells were measured before and after culturing the cells in vitro over a 6 day period in serum deprived medium containing recombinant growth factors ( interleukin 1 [ IL 1 ] , IL 3 , IL 6 , granulocyte colony stimulating factor [ G CSF ] , granulocyte macrophage [ GM ] CSF , kit ligand , and erythropoietin ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The granulomonocytic colony forming unit ( CFU GM ) assay measures committed progenitors responsive to a single species of colony stimulating activity such as granulocyte macrophage colony stimulating factor ( GM CSF ) , whereas the pre CFU assay identifies more primitive progenitors by measuring interleukin 3 ( IL 3 ) and kit ligand ( KL ) induced generation of secondary CFU GM from CD34+ , 4 hydroperoxycyclophosphamide resistant progenitors that require multiple cytokine stimuli . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Herein , we show that the cytokines interleukin ( IL ) 3 , granulocyte macrophage CSF ( GM CSF ) , and KIT ligand ( KL ) are physiological activators of VLA 4 and VLA 5 expressed by MO7e , TF 1 , and normal bone marrow CD34+ progenitor cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
A large fraction ( > 30 % ) of single cell / well sorted CD34bright / RhLA DRdull cells formed multilineage colonies after 2 to 4 weeks of stimulation with IL 3 , GM CSF , Kit ligand , and IL 6 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Further flow cytometric analysis using biotinylated growth factors showed that the IL 3R+ basophils also expressed receptors for granulocyte macrophage colony stimulating factor ( GM CSF ) , but not for IL 6 or Kit ligand . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We also demonstrated that , in the presence of interleukin 3 , interleukin 6 , granulocyte colony stimulating factor , and erythropoietin , TGF beta 1 antisense oligonucleotides or anti TGF beta serum have an additive effect with KIT ligand ( Steel factor [ SF ] ) , which suggests that they control different pathways of regulation in these conditions . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Several investigational rhHGFs , including interleukin 3 , 6 , and 11 , PIXY 321 ( a granulocyte macrophage colony stimulating factor / interleukin 3 hybrid ) , and kit ligand ( also known as `` stem cell factor ' ' ) , have the potential for affecting multiple lineages . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Bone marrow cells from each of two inbred mouse strains , C57BL / 6J and DBA / 2J , were placed into separate bioreactor cultures perfused continuously with growth medium containing erythropoietin ( Epo ) , interleukin 3 ( IL 3 ) , granulocyte macrphage colony stimulating factor ( GM CSF ) , and Kit ligand as well as 5 % CO 2 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Two cell lines , HS 5 and HS 21 , appear to be fibroblastoid and secrete significant levels of granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage CSF ( GM CSF ) , macrophage CSF ( M CSF ) , Kit ligand ( KL ) , macrophage inhibitory protein 1 alpha , interleukin 6 ( IL 6 ) , IL 8 , and IL 11 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The FLT3 / FLK2 receptor tyrosine kinase is closely related to two receptors , c Kit and c Fms , which function with their respective ligands , Kit ligand and macrophage colony stimulating factor to control differentiation of haematopoietic and non haematopoietic cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The optimal combination was found to be : Interleukin 3 ( IL 3 ) + Granulocyte Monocyte Colony Stimulating Factor ( GM CSF ) + kit ligand ( KL ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
However , combinations of cytokines present in conditioned media from the T cell lines MO or C5MJ , or defined multiple cytokine combinations containing IL 1 , IL 3 , GM CSF , kit ligand , IL 6 , and IL 9 , restored myelopoiesis in vitro . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Bacterial endotoxin ( LPS ) modulation of CSF 1 , granulocyte macrophage ( GM ) CSF , G CSF , IL 1 , TNF , and Kit Ligand receptors on murine bone marrow cells ( BMC ) in vivo and in vitro was investigated . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Although cells grown from 5 of 6 AML patients were inhibited by TGF beta 1 , cells from 1 AML patient were growth stimulated by TGF beta 1 in the presence of granulocyte macrophage colony stimulating factor ( GM CSF ) , M CSF , or mast cell growth factor ( kit ligand ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Cross reactivity between GFRs and heterologous GFs ( including IL 6 , IL 3 , GM CSF , Ep , and the kit ligand [ KL ] ) was explored by competition experiments on purified progenitors with radiolabeled and excess cold HGFs at +4 degrees C . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
By RT PCR analysis , constitutive mRNA expression of c kit , G CSF , GM CSF , IL 1 beta , IL 6 , IL 7 , IL 8 , IL 11 , Kit ligand ( KL ) , LIF , M CSF , MIP 1 alpha , TGF beta , and TNF alpha was demonstrated in both cell lines , with L87 / 4 a more potent cytokine producer than L88 / 5 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Receptor transmodulation between GM CSF , IL 3 , and stem cell factor ( or kit ligand ) was not detected on CD34+ cells in 48 hour cultures . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Surprisingly , TNF alpha was a very potent stimulator of the proliferation of CD34++CD38 cells and was the most potent synergistic factor for the IL 3 induced proliferation of these cells of all cytokines tested ( IL 1 , IL 6 , granulocyte colony stimulating factor , kit ligand ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In contrast , KIT ligand ( KL ) and granulocyte macrophage colony stimulating factor ( GM CSF ) were ineffective in promoting IL 7 dependent pro B cell growth and differentiation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Granulocyte / macrophage colony stimulating factor and kit ligand cotreatment did not overcome this inhibition . ^^^ Granulocyte / macrophage colony stimulating factor and kit ligand cotreatment did not overcome this inhibition . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Combinations of cytokines , such as kit ligand ( KL ) + interleukin 3 or KL + granulocyte macrophage colony stimulating factor ( GM CSF ) upregulated the expression of CD 95 on PHPs upon in vitro culture . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
FL promotes the growth of clonogenic myeloid progenitor cells in the presence of other cytokines known to be active on myeloid progenitors , including GM CSF , interleukin 3 ( IL 3 ) , kit ligand ( KL ) , M CSF and G CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Peripheral blood blast cells were cultured in a clonogenic assay at three cell concentrations and with the following combinations of growth factors : no added growth factor ( NF ) , G CSF , GM CSF , Kit ligand ( KL ) , G CSF + KL , GM CSF + KL , and G CSF + GM CSF + KL . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Combinations of cytokines such as hepatocyte growth factor , interleukin ( IL ) 3 , IL 11 , kit ligand , GM CSF and erythropoietin were used to study the in vitro radiation dose response of human cord blood CD34+ hematopoietic progenitor cells using clonogenic survival assays . ^^^ With the combination of IL 3 , GM CSF , kit ligand and HGF , D 0 values were not significantly altered and ranged between 1 . 61 and 2 . 60 Gy . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
After two passages , total RNA was isolated from these confluent monolayers , fractionated on denaturing agarose gels , transferred to nylon membranes , and analyzed by Northern hybridization using complementary DNAs from epidermal growth factor ( EGF ) , stem cell factor , also known as Kit ligand , colony stimulating factor 1 ( CSF ) , leukemia inhibitory factor , and interleukin 6 ( IL 6 ) . ^^^ RESULT ( S ) : Human oviduct coculture cells express EGF ( kit ligand ) , CSF , leukemia inhibitory factor , and IL 6 . ^^^ Buffalo rat liver cells contain the messenger RNA transcripts for kit ligand and CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The effect of different expansion protocols on the expression levels of CD49dw / CD29 ( VLA 4 ) , CD11a / CD18 ( LFA 1 ) , CD 31 ( PECAM 1 ) , CD 44 , and CD 34 was determined after cord blood CD34+ cells were cultured for defined periods with the following : 1 ) A growth factor mix ( GFmix ) containing interleukin ( IL ) 1 , IL 3 , IL 6 , kit ligand ( KL ) , G CSF , GM CSF , and erythropoietin ( Epo ) ; 2 ) IL 3 + KL ; and 3 ) HS 5 ( a human stromal cell line supernatant ) + KL . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Other members of this family include Steel factor ( also known as mast cell growth factor , stem cell factor , and kit ligand ) and colony stimulating factor 1 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We found that anti VLA 4 treatment combined with either granulocyte colony stimulating factor ( G CSF ) treatment or kit ligand treatment leads to significant enhancement of mobilization efficiency ( fivefold to eightfold ) well above the levels produced by either cytokine alone or anti VLA 4 treatment alone . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Long term coculture studies have shown that BMEC monolayers are unique type of endothelium and can support long term proliferation of hematopoietic progenitor cells particularly megakaryocytic and myeloid progenitor cells by constitutive elaboration of lineage specific cytokines such as G CSF , GM CSF , M CSF , Kit ligand , IL 6 , FLK 2 ligand , and leukemia inhibitory factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
FL acts synergistically in vitro with a number of other hematopoietic growth factors such as IL 3 , IL 6 , IL 11 , IL 12 , KIT Ligand and GM CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
These cells were cultured with either the HS 5 stromal cell line serum deprived conditioned media supplemented with 10 ng / mL kit ligand ( HS 5CM / KL ) or a recombinant growth factor mix ( GFmix ) containing 10 ng / mL each of interleukin ( IL ) 1 , IL 3 , IL 6 , KL , granulocyte colony stimulating factor , granulocyte macrophage colony stimulating factor , and 3 U / mL of erythropoietin . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Using the adhesive interaction of a cytokine dependent human hemopoietic cell line to immobilized fibronectin , we have analyzed the requirements in divalent cations Mn2+ , Mg2+ and Ca2+ for alpha 4 beta 1 and alpha 5 beta 1 activation by `` inside out ' ' mechanisms triggered by cytokines such as granulocyte macrophage colony stimulating factor or KIT ligand , or by external conformational constraints with the function activating anti beta 1 integrin monoclonal antibody 8A2 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Medium for the ex vivo cultures contained either serum and six recombinant human hematopoietic growth factors ( GFs ) , including Flt 3 ligand ( FL ) , Kit ligand ( KL = stem cell factor ) , thrombopoietin ( Tpo ) , interleukin 3 ( IL 3 ) , granulocyte colony stimulating factor ( G CSF ) , and interleukin 6 ( IL 6 ) , or a serum free medium containing only FL , KL , and Tpo . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CD34+ cells from a total of 93 patients with either acute myeloid leukemia ( AML ; n = 25 ) , chronic myeloid leukemia ( CML ; n = 21 ) , chronic lymphocytic leukemia ( CLL ; n = 18 ) , polycythemia vera ( PV ; n = 16 ) , or myelodysplastic syndromes ( MDS ; n = 13 ) were analyzed before and in 19 patients after ex vivo expansion in the presence of multiple cytokines ( kit ligand , interleukin 3 , interleukin 6 , and granulocyte colony stimulating factor plus erythropoietin ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The CFU C content could be expanded 50 fold by culture for 1 week in serum deprived medium and the growth factors kit ligand and granulocyte macrophage colony stimulating factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In this paper we show the megakaryocytopoietic productivity of small scale in vitro serum free cultures of human CD34+ cells containing MK growth and development factor ( MGDF ) and stem cell factor ( Kit ligand ; SCF ) + / granulocyte colony stimulating factor ( G CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The polymerase chain reaction based differential display method ( DDRT PCR ) was used to identify mRNAs differentially expressed during the maturation of human CD34+ progenitor cells stimulated to differentiate in vitro towards granulomonocytic or erythroid lineages with a mixture of hemopoietins ( kit ligand + interleukin 3 + GM CSF in the absence or presence of erythropoietin , respectively ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We confirmed functionally that HGF / SF alone has no effect on proliferation of HPCs , but that when combined with granulocyte / macrophage colony stimulating factor ( GM CSF ) or interleukin 3 it acts as a synergistic proliferative factor , although not as potently as kit ligand or FLT 3 / FLK 2 ligand . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Candidate stem cells were transduced immediately after isolation or after up to 4 days of culture in serum deprived medium containing the growth factors kit ligand and granulocyte macrophage colony stimulating factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CD 38 ( ) and CD 38 ( + ) progenitors that expressed high levels of CD 34 were grown in serum deprived medium supplemented with kit ligand , flk2 / flt3 ligand , GM CSF , c mpl ligand , erythropoietin , and IL 15 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The aim of this study was to identify signal transduction pathways activated by erythropoietin ( EpO ) and erythropoietin co stimulatory factors ( kit ligand ) , insulin like growth factor , thrombopoietin , interleukin 3 and granulocyte macrophage colony stimulating factor ) in normal human bone marrow CD 34 ( + ) cells and d 11 erythroid burst forming unit derived glycophorin+ cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We previously reported that chronic myelogenous leukemia ( CML ) primitive granulocyte monocyte ( GM ) progenitors have a greatly reduced requirement for kit ligand ( KL ) to achieve optimal growth with granulocyte colony stimulating factor ( G CSF ) + granulocyte monocyte colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We studied the effect of two different cytokine combinations kit ligand ( KL ) , interleukin 3 ( IL 3 ) , and Flt 3 ligand supplemented with thrombopoietin and IL 11 ( combination 1 ) or granulocyte / macrophage colony stimulating factor ( GM CSF ) and G CSF ( combination 2 ) for their ability to affect speed and extent of engraftment using limited numbers ( 5 10 10 ( 4 ) ) of murine bone marrow ( BM ) light density ( LD ) cells or their progeny expanded ex vivo in the presence one or the other cytokine combination for 6 days . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Soluble Kit ligand ( sKitL ) , thrombopoietin ( TPO , encoded by Thpo ) and , to a lesser extent , erythropoietin ( EPO ) and granulocyte macrophage colony stimulating factor ( GM CSF ) induced the release of SDF 1 from platelets , enhancing neovascularization through mobilization of CXCR4+ VEGFR1+ hemangiocytes . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Severe combined immunodeficient ( SCID ) mice transplanted with human bone marrow were treated with human mast cell growth factor , a fusion of interleukin 3 and granulocyte macrophage colony stimulating factor ( PIXY 321 ) , or both , starting immediately or 1 month later . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Isolated cells responded to interleukin 3 , granulocyte macrophage colony stimulating factor , mast cell growth factor , and / or granulocyte colony stimulating factor alone or in combinations in short term liquid cultures . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
To determine if earlier hematopoietic cells remained after treatment with LLME , progenitor cell experiments were repeated with 0 . 5 mM LLME using recombinant human granulocyte macrophage colony stimulating factor ( rhGM CSF ) or recombinant human interleukin 3 ( rhIL 3 ) plus or minus recombinant human mast cell growth factor ( rhMGF ) as sources of colony stimulating activity . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The line grows slowly without stimulation but responds to interleukin 3 ( IL 3 ) , GM CSF and mast cell growth factor ( MGF ) , a ligand for the receptor encoded by the c kit oncogene . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The TF 1 cell line responds to rhu IL 3 , rhu GM CSF and to a lesser extent to rhu Erythropoietin ( EPO ) and mast cell growth factor ( MGF ) , but not to rhu G CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Type 1 T helper cells ( TH 1 ) produced IL 2 , interferon gamma , GM CSF , and IL 3 in response to antigen + presenting cells or to Con A , whereas type 2 helper T cells ( TH 2 ) produced IL 3 , BSF 1 , and two other activities unique to the TH 2 subset , a mast cell growth factor distinct from IL 3 and a T cell growth factor distinct from IL 2 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Characterization of the cell surface receptor for a multi lineage colony stimulating factor ( CSF 2 alpha ) . 125I Labeled colony stimulating factor ( CSF ) 2 alpha ( interleukin 3 , multi CSF , and mast cell growth factor ) was used to characterize receptors specific for this lymphokine on the cell surface of the factor dependent cell line FDC P 2 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Failure to detect T cell lymphokines ( interleukin 2 and interleukin 3 ) and presence of macrophage colony stimulating factor ( CSF 1 ) and a novel mast cell growth factor in rheumatoid synovitis . ^^^ Sephadex column chromatography of RA SF revealed that the mast cell growth factor ( approximately 6 10 10 ( 3 ) mol wt ) and the CSF ( approximately 40 and 100 10 10 ( 3 ) mol wt ) are distinct . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The T cells expressed the L3T4+ Ly 2 phenotype and secreted the lymphokines : interleukin 1 ( IL 1 ) , interleukin 2 ( IL 2 ) , gamma interferon ( IFN gamma ) , colony stimulating factor ( CSF ) , mast cell growth factor ( MCGF ) and eosinophil differentiation factor ( EDF ) in response to antigen stimulation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Demonstration of WEHI 3 growth factor activity , mast cell growth factor activity , p cell stimulating factor activity , colony stimulating factor activity , and histamine producing cell stimulating factor activity . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
These properties of CSF 2 alpha and 2 beta are similar to those reported for interleukin 3 , hematopoietic cell growth factor , mast cell growth factor , and persisting cell growth factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
This G CSF copurifies with a mast cell growth factor but can be separated from M and GM CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Taken together , these data demonstrate that IL 3 is a major early mast cell growth factor , that mast cells become more dependent on SCF with time , and that the effects of IL 3 and SCF are upregulated ( IL 4 ) or downregulated ( M CSF , GM CSF , IFN gamma ) by both growth factors and proinflammatory cytokines . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Convergence of signaling by interleukin 3 , granulocyte macrophage colony stimulating factor , and mast cell growth factor on JAK 2 tyrosine kinase . ^^^ Mast cell growth factor ( MGF ) ( also called stem cell factor ) synergizes with several lymphokines , including interleukin 3 ( IL 3 ) and granulocyte macrophage colony stimulating factor ( GM CSF ) , to promote proliferation and differentiation of certain hemopoietic progenitor cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
GM CSF or mast cell growth factor ( MGF ) , whereas it was undetectable in growth factor deprived cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We examined the morphological and phenotypic characteristics and cell cycle status of the subpopulations and determined the responsiveness of granulocyte monocyte progenitors ( colony forming units / granulocyte macrophage ) derived from each subpopulation to mast cell growth factor in combination with granulocyte ( G CSF ) or granulocyte macrophage ( GM CSF ) colony stimulating factors alone and in combination . ^^^ Mast cell growth factor addition to cultures stimulated by G CSF , GM CSF , or G CSF plus GM CSF , exerted the greatest synergistic effect ( increased colony number and size ) in the normal small and intermediate lin blast cultures , but mast cell growth factor had considerably less effect , or no effect , in cultures of comparable CML subpopulations , indicating that CML lin progenitors had a somewhat lower requirement for multiple growth factors . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In vitro response of normal and aplastic anemia bone marrow to mast cell growth factor and in combination with granulocyte macrophage colony stimulating factor and interleukin 3 . ^^^ We have examined the effect of mast cell growth factor ( MGF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and interleukin 3 ( IL 3 ) , singly or in combination , on the growth of normal and aplastic anemia ( AA ) bone marrow in clonogenic assay and long term bone marrow culture ( LTBMC ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Neutralizing antibodies to IL 3 , GM CSF , and mast cell growth factor inhibited proliferation of the factor responsive clones by 60 % to 90 % . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The inhibitory effect of IL 6 on blast cell colony formation was retained when IL 6 was combined with granulocyte colony stimulating factor , but was lost if IL 6 was used in combination with mast cell growth factor , IL 3 , granulocyte macrophage colony stimulating factor , or IL 4 . ^^^ In these cases , however , IL 6 stimulated significantly clonogenic cell growth if combined with mast cell growth factor or granulocyte macrophage colony stimulating factor . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
No increase in GM colony formation was observed , either , when FL was used in combination with other GFs , such as mast cell growth factor ( MGF ) , granulocyte colony stimulating factor ( G CSF ) , GM CSF or interleukin 3 ( IL 3 ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The present study focused on whether it is possible to expand monocytic cells from CD34+ progenitor cells by using macrophage colony stimulating factor ( M CSF ) in the absence and presence of mast cell growth factor ( MGF ) and IL 6 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The activities of a colony stimulating factor ( CSF ) , which stimulates granulocyte macrophage colony formation by mouse hemopoietic cells , and macrophage growth factor ( MGF ) , which stimulates proliferation of activated peritoneal macrophages , have been demonstrated by various criteria to reside in the same molecular species . ^^^ CSF and MGF activities of L cell conditioned medium are ascribable to a glycoprotein of mol wt approximately 60 , 000 which migrates electrophoretically with alpha globulin . ^^^ Human urinary CSF , which also possesses MGF activity , has similar properties and can be neutralized by antiserum to highly purified L cell medium CSF . ^^^ A procedure is described for the partial purification of material from L cell medium that has activity at 1 ng / ml in both MGF and CSF assays . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The hitherto known growth factors are divided into four basic groups : 1 . haematopoietic growth factors proper [ multi CSF ( IL 3 ) , GM CSF , G CSF , M CSF , erythropoietin , lymphopoietin ( IL 7 ) and megakaryopoietin ( IL 11 ) ] , 2 . cytokines ( IL 1 to IL 11 , TFN ) . , 3 . other growth factors ( PDGF , FGF , MGF ) and 4 . so called negative regulators of haematopoiesis ( IFN , MIP , TGF beta and IL 10 ) , some of which support the differentiation of stem cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
MGF alone did not promote HPP CFC colony formation by CD34+ DR CD 15 marrow cells , but synergistically augmented the ability of a combination of granulocyte monocyte colony stimulating factor ( GM CSF ) interleukin ( IL ) 3 and a recombinant GM CSF / IL 3 fusion protein ( FP ) to promote the formation of HPP CFC derived colonies . ^^^ Repeated additions of IL 3 , GM CSF , or FP alone to CD34+ DR CD 15 marrow cells in a stromal cell free culture system increased cell numbers 10 ( 3 ) fold by day 56 of long term bone marrow culture ( LTBMC ) , while combinations of MGF with IL 3 or FP yielded 10 ( 4 ) and 10 ( 5 ) fold expansion of cell numbers . ^^^ Throughout the LTBMC , cells receiving MGF possessed a higher cloning efficiency than those receiving IL 3 , GM CSF , or FP alone . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We studied the effect of MGF on the in vitro growth of clonogenic leukemic precursor cells in the presence or absence of interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and / or erythropoietin ( EPO ) . ^^^ In the presence of IL 3 and / or GM CSF , MGF weakly stimulated the colony formation by clonogenic precursor cells from patients with AML . ^^^ In contrast , in the presence of IL 3 and / or GM CSF , MGF strongly induced both size and number of leukemic colonies from patients with CML in chronic phase . ^^^ In patients with MDS , MGF strongly stimulated myeloid colony formation in the presence of IL 3 and / or GM CSF ( up to fourfold ) , and erythroid colony formation in the presence of EPO ( up to eightfold ) . ^^^ In the absence of exogenous EPO and in the presence of 10 % human AB serum , MGF in the presence of IL 3 and / or GM CSF induced erythroid colony formation from normal bone marrow and patients with MDS or CML , illustrating that MGF greatly diminished the EPO requirement for erythroid differentiation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
A macrophages growth factor ( MGF ) was purified and , at the final step of purification on HPLC , eluted at the same position as a colony stimulating factor ( CSF ) , suggesting that MGF is identical with CSF . ^^^ In the present study , we examined the relationship between proliferation and fusion of macrophages using purified CSF ( MGF ) and 1 alpha , 25 dihydroxyvitamin D 3 [ 1 alpha , 25 ( OH ) 2D3 ] . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
When either IL 4 or MGF was added to the combination of IL 3 , GM CSF , G CSF , and IL 6 , a statistically significant increase in the colony number was observed . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
MGF alone had a statistically significant stimulatory effect on GM colony formation , as also did interleukin 3 ( IL 3 ) and GM colony stimulating factor ( GM CSF ) , although the progenitors could form colonies spontaneously as well . ^^^ When MGF was combined with either IL 3 or GM CSF the effect was additive and was as great as that achieved with a mixture of IL 3 , GM CSF , G CSF and IL 6 . ^^^ MGF was the most potent single factor in PV , while GM CSF was most effective in idiopathic myelofibrosis and both IL 3 and GM CSF in CML . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
When used in combination with plateau concentrations of pokeweed mitogen mouse spleen cell conditioned medium or granulocyte macrophage colony stimulating factor ( CSF ) , MGF enhanced in greater than additive fashion colony formation by CFU GM . ^^^ MGF did not enhance Macrophage CSF stimulated colony numbers or size . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Individual day 14 to 18 CFU GEMM or BFU E colonies were removed from primary cultures and reseeded into secondary methylcellulose cultures containing a combination of Epo , MGF , and rhu granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The MO mitogenic principle of MGF was identified as the murine , macrophage specific colony stimulating factor , CSF 1 ( M CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
To examine the relationship between the bone resorbing factor and other protein factors produced by spleen cells , the colony stimulating factor ( CSF ) , the differentiation inducing factor ( DIF ) , the macrophage fusion factor ( MFF ) , and the macrophage growth factor ( MGF ) were purified from 2 . 68 liters of conditioned medium of mouse spleen cell cultures treated with concanavalin A . ^^^ The DIF was successfully separated from CSF and MGF on HPLC . ^^^ CSF coincided with MGF on HPLC , but MFF disappeared before application to HPLC . ^^^ Only the DIF exhibited bone resorbing activity , whereas CSF and MGF did not . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Optimal outgrowth was achieved by initially culturing CD34+ cells for 5 days in medium containing GM CSF , MGF and TNF alpha . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The activity of the colony stimulating factor ( SCF ) was measured in kidney subcellular fractions in mice . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We show that recombinant rat SCF ( rrSCF 164 ) administered to weanling rats selectively induces an increase in thymocyte progenitor activity in the spleens of treated rats as compared to rats treated with vehicle , polyethylene glycol ( PEG ) conjugated rat albumin , or recombinant human granulocyte colony stimulating factor ( rhG CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
MGF synergized with interleukin 3 ( IL 3 ; four out of 10 cases ) , granulocyte macrophage colony stimulating factor ( GM CSF ; three out of 10 cases ) , granulocyte colony stimulating factor ( G CSF ; six out of 10 cases ) , macrophage colony stimulating factor ( M CSF ; one out of 10 cases ) and erythropoietin ( EPO ; one out of 10 cases ) when added to culture in combination . ^^^ Antibodies against GM CSF , M CSF , G CSF , and IL 6 did not inhibit the MGF response , suggesting that the stimulative effect of MGF was not mediated through autocrine release of those cytokines . ^^^ The MGF dose response curve in the presence of IL 3 or GM CSF resulted in a higher plateau of DNA synthesis , however no shift in the dose response was apparent . ^^^ The respective reciprocal dose response relations to GM CSF , IL 3 , or G CSF were similarly elevated when MGF was added . ^^^ MGF did not alter IL 3 and GM CSF receptor expression , nor did IL 3 , GM CSF , G CSF , TNF , or IL 4 influence MGF binding to AML cells . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Antibodies against interleukin 1 ( IL 1 ) , IL 3 , granulocyte macrophage colony stimulating factor ( GM CSF ) , and Epo could not abolish the enhancing effect of MGF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
MGF alone or in combination with MoT cell line conditioned medium ( MoCM ) or granulocyte macrophage colony stimulating factor ( GM CSF ) + interleukin 3 ( IL 3 ) significantly increased the proliferative capacity of erythropoietin ( EPO ) dependent CML and normal BFU E . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Other growth factors such as PIXY , interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) and granulocyte CSF ( G CSF ) and the combination of MGF plus PIXY were also tested . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Preincubation of the cells with MGF in suspension did not sensitize them to the effect of IL 3 , granulocyte macrophage colony stimulating factor ( GM CSF ) , G CSF , or IL 4 in a clonogenic cell culture assay . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In addition , a number of cytokines such as lymphotoxin and interferon gamma ( IFN gamma ) , are expressed exclusively in thymocytes whereas others such as stem cell factor ( SCF ) , IL 1 receptor antagonist 2 ( IRAP 2 ) and granulocyte macrophage colony stimulating factor ( GM CSF ) are produced only in stromal cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The authors present an account of haematopoietic growth factors which include the stem cell factor ( SCF ) , factors stimulating granulocytomacrophage colonies ( GM CSF ) , granulocyte ( G CSF ) , monocytomacrophage ( M CSF ) factors , erythropoietin ( EPO ) and also interleukin ( IL ) 1 to 11 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Human SCF / KL alone or in combination with the hematopoietic growth factors , interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and IL 6 , stimulated proliferation of these megakaryocytic cell lines . ^^^ Addition of SCF / KL with IL 3 or GM CSF to these cultures resulted in a more marked marrow megakaryocytic cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
When AML blast progenitors were grown either in the presence of fetal calf serum ( FCS ) alone or with one of the following : phytohemagglutinin leukocyte conditioned medium ( PHA LCM ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , G CSF , interleukin 3 ( IL 3 ) , or stem cell factor ( SCF ) , addition of 100 ng / mL sIL 1R or IL 1RA inhibited blast colony formation by 3 % to 96 % and 2 % to 97 % , respectively . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
SCF interacted with erythropoietin ( Epo ) and granulocyte colony stimulating factor ( G CSF ) to maintain large numbers of cells as well as to generate a twofold to threefold increase in CFC in the case of Epo , and a 10 fold increase in CFC in the case of G CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Using optimal concentrations of colony stimulating factors ( CSF ) without added SCF approximately 2 . 5 % of cells formed colonies . ^^^ SCF also had CSF activity on this population , up to 0 . 5 % of cells forming small colonies in response to SCF alone . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
SCF , in combination with erythropoietin ( Epo ) , interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and granulocyte colony stimulating factor ( G CSF ) , increased the number of hematopoietic colonies formed in a semisolid medium by AA marrows . ^^^ Proliferation of AA cells cultured in a liquid medium containing SCF together with Epo , IL 3 , GM CSF , and G CSF approached 70 % of the control level , as measured by 3H thymidine incorporation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
To determine if neoplastic human hematopoietic cells would also respond to SCF , we cultured marrow mononuclear cells from 20 patients with newly diagnosed acute myelogenous leukemia ( AML ) and two normal donors with SCF , interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , or combinations of growth factors in semisolid medium , and assessed colony growth . ^^^ Our results show that SCF alone or in combination with IL 3 or GM CSF increased both the number and size of colonies in 10 of the patients . ^^^ These results identify c kit receptors on human AML blasts , and indicate that SCF acts synergistically with IL 3 or GM CSF to stimulate colony growth from the marrow cells of a portion of patients with AML . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We have investigated the proliferation of 31 myeloid leukemia cell lines as well as fresh myeloid leukemic blasts from 17 patients in a 72 hour 3H thymidine uptake assay in the presence of various concentrations of recombinant human ( rh ) SCF alone or in combination with saturating concentrations of granulocyte macrophage colony stimulating factor ( GM CSF ) , G CSF , M CSF , interleukin 3 ( IL 3 ) , or erythropoietin ( EPO ) . ^^^ Synergistic activities of SCF were found with G CSF , GM CSF , EPO , and IL 3 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
A combination of 6 HGF , ie , interleukin 1 beta ( IL 1 ) , IL 3 , IL 6 , granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage CSF ( GM CSF ) , and stem cell factor ( SCF ) , was identified as the most potent combination of those tested . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We and others have recently shown that the c kit gene product is expressed on human megakaryocytes and that soluble KL / SCF in combination with granulocyte macrophage colony stimulating factor , interleukin 3 ( IL 3 ) , or IL 6 increased megakaryocyte progenitor colony formation ( CFU MEG ) and stimulated mature megakaryocytes . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
IL 3 and stem cell factor / mast cell growth factor ( SCF ) showed only a marginal effect , and granulocyte macrophage and macrophage CSFs ( GM CSF and M CSF , respectively ) were inactive . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
SCF increased colony forming unit granulocyte macrophage ( CFU GM ) formation from CB CD34+ cells compared to unconditioned media and had a significant additive increase with IL 3 ( p = 0 . 006 ) and granulocyte colony stimulating factor ( p = 0 . 03 ) . ^^^ In this study , we compared the success of separating very early hematopoietic progenitor cells ( CD34+ ) from both cord blood ( CB ) and adult bone marrow ( ABM ) and their differential response to SCF , PIXY 321 , and other later acting colony stimulating factors ( CSF ) . ^^^ Briefly , CD34+ cells were isolated from CB and ABM with an anti CD 34 MAb , HPCA 1 , and incubated with various combinations of SCF , PIXY 321 , and other CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Conversely , the ability of SCF to induce primary colony formation by AML clonogenic cells ( CFU L ) was lower than that of granulocyte macrophage colony stimulating factor ( GM CSF ) and 5637 SN in all but four cases . ^^^ However , SCF potentiated the stimulatory effect of GM CSF , G CSF , and IL 3 on both 3H TdR incorporation and colony formation . ^^^ A further increment was obtained by combinations of SCF with GM CSF , G CSF , or IL 3 , and this was significantly more effective than 5637 SN . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In the presence of SCF alone , or in combination with granulocyte macrophage colony stimulating factor or interleukin 3 , there was less than a 17 % increase in the colony growth of Du 4475 , H 69 , or H 128 cell lines . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Bone marrow cells were plated in vitro for colony forming unit granulocyte macrophage ( CFU GM ) ( in the presence of granulocyte macrophage colony stimulating factor [ GM CSF ] ) , and for burst forming unit erythroid ( BFU E ) and CFU granulocyte , erythroid , monocyte , megakaryocyte ( CFU GEMM ) colonies ( in the presence of erythropoietin and interleukin 3 [ IL 3 ] ) , with or without 20 ng / mL of SCF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In serum deprived conditions , no colonies were formed in the presence of macrophage colony stimulating factor ( M CSF ) , but when M CSF was combined with SCF , a marked change was noted in that large colonies were produced containing predominantly macrophages . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Non adherent cord blood and bone marrow mononuclear cells were analyzed by multiparameter flow cytometry before and at day 2 , 4 , 7 , and 11 of culture in recombinant interleukin 3 ( IL 3 ) and granulocyte colony stimulating factor ( G CSF , cord blood ) or stem cell factor ( SCF ) , IL 3 and granulocyte macrophage colony stimulating factor ( GM CSF , BM ) to assess the differentiation and maturational pathway of myeloid cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
SCF , in combination with interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , or granulocyte colony stimulating factor ( G CSF ) caused CD34+ cells to form increased numbers of granulocyte macrophage colonies ( CFU GM ) , and to form macroscopic erythroid burst forming units ( BFU E ) in the presence of IL 3 , erythropoietin ( Epo ) , and SCF . ^^^ CD34+lin cells cultured in liquid medium containing SCF combined with IL 3 , GM CSF , or G CSF gave rise to increased numbers of CFC . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Combination of SCF with granulocyte , granulocyte macrophage , or multipotential colony stimulating factors , but not macrophage colony stimulating factor , resulted in enhancement of colony size . ^^^ Granulocyte colony stimulating factor enhanced cell proliferation initiated by SCF , but not vice versa , and resulted in a 10 fold increase in colony cell numbers and a 7 fold increase in progenitor cells in blast colonies . ^^^ No evidence was obtained that SCF , alone or in combination with granulocyte colony stimulating factor , could stimulate self generation by blast colony forming cells . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Colony forming assays of all experimental groups showed a synergistic increase in colony forming unit granulocyte macrophage ( CFU GM ) in the marrow , but not in peripheral blood , after coincubation with SCF plus granulocyte macrophage CSF ( GM CSF ) as opposed to GM CSF alone , showing anatomic compartmentalization between a more primitive marrow CFU GM subset and a more mature peripheral blood CFU GM subset . ^^^ In vivo daily administration of SCF plus GM CSF results in a synergistic increase in marrow neutrophils , but not the striking synergistic increase in circulating neutrophils that is observed with SCF plus G CSF . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
While SCF alone had little colony stimulating activity under fetal bovine serum ( FBS ) deprived culture conditions , SCF synergized with erythropoietin ( Epo ) , granulocyte / macrophage colony stimulating factor ( GM CSF ) , and interleukin 3 ( IL 3 ) to stimulate colony growth . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In clonal cultures of normal mouse marrow cells , combination of granulocyte , granulocyte macrophage , or multipotential colony stimulating factor ( G CSF , GM CSF , or multi CSF , respectively ) with stem cell factor ( SCF ) did not alter the number of blast colonies stimulated to develop compared with SCF alone but induced an up to 25 fold increase in their mean cell content and an up to 6 fold increase in their mean progenitor cell content . ^^^ However , combination of GM CSF or multi CSF with SCF significantly increased the relative frequency of granulocytic progenitors and , for multi CSF , also of eosinophil progenitor cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In vitro , SCF alone stimulated little growth of hematopoietic colony forming cells from baboon marrow , but did increase the number of colonies formed in response to erythropoietin ( Epo ) , interleukin 3 ( IL 3 ) , and granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The presence of muscular CP isoenzyme in the CSF of stroke patients was explained by increased blood brain barrier permeability , and blood admixture to SCF . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
After a control period during which both lateral ventricles were perfused with mock CSF with the same composition , the drug under study was added to one side ( experimental side ) while the other side was perfused further with the control mock SCF ( control side ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Synergistic activities were seen when FL was combined with G CSF , GM CSF , IL 3 , or stem cell factor ( SCF ) . ^^^ In the presence of suboptimal and optimal concentrations of G CSF , GM CSF , IL 3 , SCF , or a combination of all factors , FL strongly enhanced the number of leukemic colonies ( up to 18 fold ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In this study , we show a dose dependent response of AS 101 in the induction of high secretion levels of IL 6 , IL 3 , granulocyte macrophage colony stimulating factor ( GM CSF ) , and stem cell factor ( SCF ) . ^^^ We suggest that the endogenous production of cytokines such as IL 1 , IL 6 , IL 3 , SCF , and GM CSF in mice treated with AS 101 offers protection to circulating blood elements and ameliorates the reconstitution of megakaryocytic and erythroid progenitors . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The combined action of stem cell factor ( SCF ) and granulocyte colony stimulating factor ( G CSF ) on granulocyte recovery and infection with Candida albicans was studied . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
However , whereas IL 1 , IL 2 , IL 3 , IL 4 , granulocyte colony stimulating factor , and granulocyte macrophage colony stimulating factor were ineffective , IL 7 was a potent cytokine , alone or in synergy with stem cell factor ( SCF ) to induce immature thymocyte proliferation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We analyze here the amplification potential of CD34+ cord blood cells in liquid cultures stimulated with stem cell factor ( SCF ) in combination with interleukin 3 ( IL 3 ) , erythropoietin ( Epo ) or granulocyte colony stimulating factor ( G CSF ) under serum deprived conditions . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
These experiments revealed that there is an inverse correlation between the SCF R density on the cells and the doses of granulocyte macrophage colony stimulating factor ( GM CSF ) in culture medium ; the lower the dose , the higher the density of SCF R on the cells . ^^^ More detailed analyses showed that , in contrast to SCF which rapidly downregulates its own receptor , GM CSF does not alter the measurable level of SCF R in an exposition period of 60 minutes , which suggests that the internalization or shedding of the receptor is not the mechanism of action . ^^^ Since the most striking difference regarding density of SCF R between GM CSF treated and untreated cells was observed on day 2 , the modulation of c kit oncogene protein by GM CSF likely occur prior to expression of protein onto the cell surface . ^^^ In order to exclude the possibility that altered cell viability due to insufficient GM CSF content in culture medium might be responsible for the increased SCF R densities on GM CSF dependent cells , we subsequently generated a GM CSF independent subclone which still responded to GM CSF as well as the dependent did . ^^^ Thus our data suggest that GM CSF is directly involved in the regulation of SCF receptor density on GM / SO cells . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
At concentrations ranging from 0 . 1 to 10 . 0 micrograms / ml , it significantly inhibited colony forming units granulocyte macrophage ( CFU GM ) in the presence of either fetal calf serum alone , erythropoietin , leukocyte conditioned medium prepared with phytohemagglutinin , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) , or stem cell factor ( SCF ) , in a dose dependent fashion . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) was found to stimulate the growth of the haemopoietic cell line FDC P 1 in synergy with either interleukin 3 ( IL 3 ) or granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^ Synergy between SCF or M CSF with IL 3 or GM CSF in FDC P 1 cells : a sensitive assay of transforming mutations of c fms . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Growth factor enhancement of CFU GM yield was obtained only by stem cell factor ( SCF ) at day 7 ; SCF , as well as granulocyte macrophage colony stimulating factor ( GM CSF ) and interleukin 3 ( IL 3 ) , had an enhancing effect at days 14 and 21 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
LTCBC initiated with CD34+HLA DR+ and CD34+HLA DR CB cells were examined over a period of 8 weeks for the production of assayable burst forming units erythroid ( BFU E ) , colony forming units granulocyte / macrophage ( CFU GM ) , and colony forming units mixed ( CFU GEMM ) in response to repeated additions of stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , IL 6 , and either erythropoietin ( Epo ) or granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , the ligand of the c kit receptor , is a potent enhancing cytokine for haematopoietic cells in the presence of IL 3 , GM CSF and erythropoietin ( Epo ) . ^^^ In the clonogenic assays of 63 MDS patients , the addition of rh SCF + GM CSF and / or IL 3 induced a significant increase ( p < 0 . 001 ) in the number and size of CFU GM . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Putative cytokines and growth factors , such as granulocyte colony stimulating factor ( CSF ) , macrophage CSF , granulocyte macrophage CSF , stem cell factor ( SCF ) , interleukin ( IL ) 6 , and IL 3 , do not possess HSC chemotactic activity . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Four CD34+ cell populations , ie , ( 1 ) CD 38 , HLA DR+ , ( 2 ) CD 38 , HLA DR , ( 3 ) CD38+ , HLA DR , and ( 4 ) CD38+ , HLA DR+ cells , were sorted as single cells into 96 well flat bottom culture plates containing long term culture medium supplemented with interleukin 3 , interleukin 6 , stem cell factor ( SCF ) , granulocyte macrophage colony stimulating factor , erythropoietin , basic fibroblast growth factor ( bFGF ) , and insulin like growth factor 1 ( IGF 1 ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The effects of recombinant human stem cell factor ( SCF / c kit ligand ) , interleukin 3 ( IL 3 ) and granulocyte / macrophage colony stimulating factor ( GM CSF ) on erythroid colony formation by non phagocytic mononuclear cells ( MNC ) and CD34+ cells derived from normal human bone marrow ( BM ) , peripheral blood ( PB ) and umbilical cord blood ( CB ) were studied using a methylcellulose culture containing recombinant human erythropoietin ( Epo ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
SCF in combination with G CSF significantly augmented the proliferation of GM CFCs . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Finally , involvement of an autocrine loop of activation induced by MS 5 was excluded : RT PCR analysis did not detect increased transcripts for GM CSF , IL 3 , IL 6 , SCF , or Epo in UT 7 cells cocultured for 2 to 6 days with MS 5 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In this study , we have attempted to relate the murine HPP CFC , stimulated by various combinations of growth factors ( GFs ) CSF 1 , GM CSF , IL 3 , IL 6 , IL 1 , stem cell factor ( SCF ) , and transforming growth factor beta ( TGF beta ) and by CSF 1 , GM CSF , and IL 3 on their own , to these known progenitors . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The hemopoietic cytokines IL 3 ( interleukin 3 ) , IL 5 and GM CSF ( granulocyte macrophage colony stimulating factor ) are involved in the regulation of basophils ( and eosinophils ) , whereas the ligand for c kit , SCF ( stem cell factor ) is a mast cell specific agonist . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
It is known that , although SCF and bFGF have limited clonogenic activity on their own , they can augment colony stimulating factor ( CSF ) mediated progenitor cell growth . ^^^ In this study , we show that clonogenic growth derived from low density bone marrow cells stimulated by GM CSF is significantly augmented ( P < . 001 ) in the presence of maximal ( 100 ng / mL ) concentrations of SCF in combination with 100 ng / mL of bFGF . ^^^ When CD34+ cells are used , the synergistic effect of bFGF and SCF for GM CSF mediated progenitor cell growth is further increased , resulting in as much as a sevenfold increase in detectable colony forming units granulocyte macrophage ( P < . 001 ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
With a highly enriched population of these cells we have found that although GM CFC are equally responsive to macrophage colony stimulating factor ( M CSF ) and stem cell factor ( SCF ) in terms of DNA synthesis , M CSF stimulated the development of colonies containing macrophages in soft gel assays , while SCF promoted neutrophilic colony formation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We demonstrated that MDS marrow hematopoietic progenitor cells reactive to GM CSF or EPO were additionally stimulated with early acting hematopoietic growth factors including IL 6 , IL 3 and SCF in a fashion similar to those of normal individuals . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
However , if combined with GM CSF , which per se downregulates the SCF R on these cells as well , TNF alpha antagonized the effect of GM CSF and slightly increased the density of SCF R . ^^^ Yet the cells incubated for 24 hours in medium without cytokines invariably expressed a higher level of SCF R than the cells incubated in the presence of TNF alpha and GM CSF , either alone or in combination . ^^^ In contrast , no such morphological changes could be observed in the presence of GM CSF or SCF . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Immediately after marrow transplant , the mice received SCF , interleukin 3 ( IL 3 ) , and granulocyte macrophage colony stimulating factor ( GM CSF ) alone or in combination daily for 6 days . ^^^ In contrast , treatment of primary recipients with SCF and GM CSF or IL 3 or with all three cytokines resulted in a synergistic increase of CFU S in secondary recipients , indicating increased pre CFU S levels . ^^^ We conclude that SCF with IL 3 and / or GM CSF increases pre CFU S proliferation . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
When combined with subplateau concentrations ( 0 . 1 microgram / L ) of IL 3 or granulocyte macrophage colony stimulating factor , SCF had an additive or synergistic effect on clonogenic maturation of granulocyte macrophage colony forming unit and CFU MIX . ^^^ When combined with higher concentrations ( 5 . 0 micrograms / L ) of IL 3 or granulocyte macrophage colony stimulating factor , SCF generally did not enhance colony formation but did increase the number of cells per colony . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In this study , we have investigated the effects of ECM molecules , particularly of FN , on the proliferation of a myeloid leukemia cell line , M07E , which proliferates in response to either human granulocyte / macrophage colony stimulating factor ( GM CSF ) or stem cell factor ( SCF ) . ^^^ The [ 3H ] thymidine incorporation and cell enumeration assays showed that FN strikingly inhibited GM CSF or SCF induced proliferation of M07E cells in a dose dependent manner , whereas little or no inhibition was induced by collagen types 1 and 4 . ^^^ The growth suppression of M07E cells was not due to the inhibitory effect of FN on ligand binding or very early events in the signal transduction pathways from the GM CSF or SCF receptors . ^^^ DNA content analysis using flow cytometry after staining with propidium iodide revealed that the treatment of M07E cells with FN did not block the entry of the cells into the cell cycle after stimulation with GM CSF or SCF , whereas the treatment resulted in the appearance of subdiploid peak . ^^^ Furthermore , FN was found to induce oligonucleosomal DNA fragmentation and chromatin condensation in the cells even in the presence of GM CSF or SCF , suggesting the involvement of programmed cell death ( apoptosis ) in the FN induced growth suppression . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Furthermore , TNF alpha blocked all colony formation stimulated by SCF in combination with granulocyte colony stimulating factor ( CSF ) or CSF 1 . ^^^ The synergistic effect of SCF observed in combination with GM CSF or IL 3 was also inhibited by TNF alpha , resulting in colony numbers similar to those obtained in the absence of SCF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The number of the different haemopoietic progenitors / 1 10 10 ( 3 ) CD34+ cells , except CFU meg , was significantly higher in fetal blood than in adult bone marrow in cultures stimulated by any combination of cytokines including interleukin 3 ( IL 3 ) , granulocyte / macrophage colony stimulating factor ( GM CSF ) or stem cell factor ( SCF ) plus erythropoietin ( Epo ) . ^^^ Addition of optimal concentrations of IL 3 , GM CSF or SCF , alone or in various combinations , to Epo containing cultures induced a significant increase in both the number and size of fetal CFU GEMM , and CFU GM , and a parallel decrease of fetal BFU E . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Circulating haemopoietic progenitor cells from premature infants were assessed for their ability to respond to interleukin 3 , granulocyte macrophage colony stimulating factor and stem cell factor ( SCF ) in vitro . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We treated mice with granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) to mobilize hematopoietic progenitor cells into the peripheral blood . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Recombinant human SCF has major clinical potential through its synergy with other factors , especially recombinant human granulocyte colony stimulating factor , to enhance mobilization of PBPC . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Furthermore , PB CD34+ cells were expanded in liquid culture in the presence of granulocyte macrophage colony stimulating factor ( GM CSF ) or G CSF alone or combined with interleukin 3 ( IL 3 ) , stem cell factor ( SCF ) , interleukin 1 ( IL 1 ) , interleukin 6 ( IL 6 ) . ^^^ The combination of GM CSF , IL 3 , SCF , IL 1 and IL 6 produced the maximum increase of both mononuclear cells ( 30 fold ) and granulocyte macrophage colony forming units ( CFU GM ) ( 4 . 6 fold ) after 7 days of cultures ; yet after 14 days a strong decrease of the CFU GM occurred . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
SCF enabled neutrophil and neutrophil / macrophage mixed progenitors to respond to granulocyte / macrophage colony stimulating factor ( GM CSF ) or interleukin 3 ( IL 3 ) and to develop the colony and further cluster formation . ^^^ The neutrophil colonies stimulated by GM CSF or IL 3 consisted mainly of immature cells , while the colonies stimulated by granulocyte colony stimulating factor ( G CSF ) consisted of mature neutrophils irrespective of the addition of SCF . ^^^ In macrophage and eosinophil lineages , SCF augmented the colony formation in the presence of GM CSF or IL 3 , whereas the enhancement of total progenitor cell growth ( colonies plus clusters ) was not so marked as compared with the neutrophil lineage . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , a potent colony stimulating factor , enhanced both erythroid and nonerythroid colony formation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Although no significant effects were observed on granulocyte macrophage CSF ( GM CSF ) interleukin 3 or stem cell factor ( SCF ) induced proliferation , the combinations of G CSF and each of these cytokines were all inhibited . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The combination of SCF and Epo or granulocyte macrophage colony stimulating factor ( GM CSF ) supported the production and maintenance of CFC from CD34+Rh 123low cells > 28 days compared with only 21 days for the CD34+Rh 123high cells . ^^^ The addition of anti GM CSF receptor Ab to cultures of CD34+Rh 123high and low cells supplemented with SCF , interleukin 3 ( IL 3 ) , and IL 6 resulted in an initial 10 fold decrease in CFC in cultures of both the CD34+Rh 123high and low cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The expression of an exogenous c myc proto oncogene or a 12H ras oncogene lowered the requirement of FDC P 1 cells for IL 3 or GM CSF , in a similar manner to M CSF or SCF addition . ^^^ These results strongly support a role for ras and myc in the synergistic action of M CSF and , by implication , of SCF , which implies that these signalling intermediates are rate limiting for the action of IL 3 and GM CSF and possibly other haemopoietic growth factors . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Because of the importance of cytokines in the regulation of allergic inflammation , we investigated the effects of SCF , IL 3 , IL 5 and GM CSF on immunological histamine release from sensitized human lung fragments as well as human leukocytes . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The liquid culture of PB CD34+ cells in the presence of granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) , and stem cell factor ( SCF ) resulted in a marked and long lasting increase of colony forming units granulocyte / macrophage ( CFU GM ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Based on our recent observations that recombinant rat interleukin 3 ( IL 3 ) , human interleukin 6 ( IL 6 ) and murine granulocyte macrophage colony stimulating factor ( GM CSF ) possessed different degrees of suppressive activities on the proliferation of LT 12 cell line derived from BNML rat leukemic model , SCF was evaluated alone and in combination with either IL 3 , IL 6 or GM CSF for effects on leukemopoiesis in vitro . ^^^ The results indicated that SCF alone had suppressive effect on DNA synthesis and colony forming unit leukemic blast ( CFU L ) in LT 12 cells . 100ng / ml of SCF caused substantial reduction in colony number and 3H TdR uptake although this suppression was of lower magnitude than those induced by IL 3 , IL 6 or GM CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We have previously examined the effects of granulocyte colony stimulating factor ( G CSF ) alone or in combination with either stem cell factor ( SCF ) or interleukin 11 ( IL 11 ) on in vivo neonatal rat hematopoiesis . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The current study asked whether survival could be further improved by treating allograft recipients with recombinant canine granulocyte colony stimulating factor ( G CSF ) , stem cell factor ( SCF ) , or G CSF / SCF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We sorted single cells from four populations of CD34+ cells from fetal bone marrow : ( 1 ) CD 38 HLA DR , ( 2 ) CD 38 HLA DR+ , ( 3 ) CD38+ HLA DR , and ( 4 ) CD38+ HLA DR+ into liquid culture media supplemented with interleukin 3 ( IL 3 ) IL 6 , stem cell factor ( SCF ) , granulocyte macrophage colony stimulating factor , erythropoietin , basic fibroblast growth factor ( bFGF ) and insuline like growth factor ( IGF 1 ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
RNA analysis showed that stromal cells transcribed , in addition to SCF , messages for granulocyte colony stimulating factor ( G CSF ) , granulocyte monocyte CSF ( GM CSF ) , interleukin 6 ( IL 6 ) , and transforming growth factor beta ( TGF beta ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
To examine the role of stem cell factor ( SCF ) or its receptor in this disorder , we investigated the stimulatory effects of recombinant canine SCF ( rc SCF ) on in vitro marrow cultures , cloned and sequenced the grey collie SCF gene , and treated three grey collies with rc SCF , either alone or in combination with recombinant canine granulocyte colony stimulating factor ( rcG CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Using clonogenic assay we investigated the effect of stem cell factor ( SCF ) on the in vitro growth of clonogenic precursor cells from acute myeloid leukemia ( AML ) and myelodysplastic syndromes ( MDS ) in the presence or absence of recombinant human erythropoietin ( rhEpo ) or recombinant human granulocyte colony stimulating factor ( rhG CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We studied the synergistic effects of stem cell factor ( SCF ) and other burst promoting activities ( BPAs ) such as interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , or IL 9 on proliferation of human peripheral blood derived highly purified progenitors . ^^^ SCF , IL 3 , GM CSF , and IL 9 showed significant BPA when CD34+HLA DR+ cells were used as the target population . ^^^ SCF and IL 9 showed much weaker BPA than that of IL 3 or GM CSF . ^^^ However , GM CSF showed a significant additive effect with IL 9 or SCF . ^^^ Also , a distinct additive effect between SCF and IL 3 or GM CSF on erythrocyte containing mixed colony formation was observed . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
For its effect , SCF requires the synergistic action of erythropoietin ( Epo ) , granulocyte colony stimulating factor ( G CSF ) , or interleukin 3 ( IL 3 ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
UT 7 cultured with SCF and UT 7scf9 cultured without cytokines expressed GM CSF , and anti GM CSF neutralizing antibody partially inhibited their growth . ^^^ These results suggest that SCF stimulated UT 7 proliferation partially through the autocrine loop of GM CSF , and UT 7scf9 expressed SCF mostly as a membrane bound form , which transduces its growth signal through c kit receptor as they aggregate by cell to cell interaction . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In vitro colony assay revealed that the combination of PSK and any of GM CSF , IL 3 or stem cell factor ( SCF ) showed a greater increase in colony numbers than when these materials were administered individually , although G CSF did not show a synergistic effect with PSK . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Modulation of human cord blood progenitor cell growth by recombinant human interleukin 3 ( IL 3 ) , IL 6 , granulocyte macrophage colony stimulating factor ( GM CSF ) and stem cell factor ( SCF ) in serum supplemented and serum free medium . ^^^ We evaluated the growth of cord blood myeloid progenitors or colony forming units granulocyte macrophage ( CFU GM ) and their response to various recombinant growth factors or colony stimulating factors ( CSFs ) : interleukin 3 ( IL 3 ) , IL 6 , granulocyte macrophage CSF ( GM CSF ) and stem cell factor ( SCF ) . ^^^ The association of IL 3 , IL 6 , GM CSF and SCF induced significantly more CB day 14 CFU GM than HPCM . ^^^ In conclusion : a ) day 14 / day 7 CFU GM ratio was higher in CB than in BM indicating that CB CFU GM are more primitive than BM CFU GM ; b ) FCS can be successfully replaced by serum free medium ; c ) FCS contains inhibitors of day 14 CFU GM and among them TGF beta ; and d ) the association IL 3 , SCF , GM CSF and IL 6 seems able to totally overcome the inhibitory effect of FCS . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The selected cells were expanded for two weeks in Iscove ' s medium supplemented with 20 % FBS and various combinations of interleukin 3 ( IL 3 ) , granulocyte colony stimulating factor ( G CSF ) , stem cell factor ( SCF ) and interleukin 6 ( IL 6 ) added either simultaneously or sequentially . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In this study , we investigated the expression of receptors for G CSF , GM CSF , SCF , IL 3 , and IL 7 on acute lymphoblastic leukemia ( ALL ) blasts and the effects of these growth factors ( GF ) on ALL blast colony formation . ^^^ Receptor binding for myeloid associated GF was observed in the majority of precursor B ALL ( G CSF = 100 % , GM CSF = 65 % , IL 3 = 83 % , SCF = 74 % ) , but not in T ALL . ^^^ Expression of receptors for G CSF , GM CSF , IL 3 , and SCF was not associated with expression of myeloid antigens , or with specific cytogenetic abnormalities . ^^^ We conclude that ALL blast cells have receptors not only for IL 7 , but also for G CSF , GM CSF , SCF , and IL 3 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Specifically , TNF alpha is a potent inhibitor of the clonal growth of primitive and committed murine and human bone marrow progenitors in combination with multiple cytokines , including granulocyte colony stimulating factor ( G CSF ) , CSF 1 , erythropoietin ( Epo ) , stem cell factor ( SCF ) , and flt 3 ligand ( FL ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Inclusion of SCF , together with IL 3 , GM CSF or both cytokines stimulated growth of these cells over 14 day period . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Specificity of CD 64 for the granulo monocytic lineage was shown by demonstrating that colonies arising from CD34+ , CD64+ cells consisted of 98 % + / 2 % colony forming unit granulocyte macrophage ( CFU GM ) in semisolid medium containing stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , IL 6 , granulocyte macrophage colony stimulating factor ( GM CSF ) , and erythropoietin ( EPO ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Whereas FL was a weak proliferative stimulator alone , it potently synergized with a number of other HGFs , including all four colony stimulating factor ( CSF ) , interleukin ( IL ) 6 , IL 11 , IL 12 , and stem cell factor ( SCF ) , to promote the colony formation of Lin Sca 1+ , but not Lin Sca 1 or erythroid progenitor cells . ^^^ The synergistic activity of FL was concentration dependent , with maximum stimulation occurring at 250 ng / ml , and was observed when cells were plated at a concentration of one cell per culture , suggesting that its effects are directly mediated . 2 wk of treatment with FL in combination with IL 3 or SCF resulted in the production of a high proportion of mature myeloid cells ( granulocytes and macrophages ) , whereas the combination of FL with G CSF , IL 11 , or IL 12 resulted predominantly in the formation of cells with an immature blast cell appearance . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We show that treatment of UT 7 cells with EPO , GM CSF or stem cell factor ( SCF ) rapidly induces phosphorylation of p95B raf as indicated by a shift of electrophoretic mobility . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In the present study , we investigated the capacity of porcine microvascular endothelial cells ( PMVECs ) together with combinations of cytokines ( granulocyte macrophage colony stimulating factor [ GM CSF ] + stem factor [ SCF ] , interleukin 3 [ IL 3 ] + SCF + IL 6 , and GM CSF + IL 3 + SCF + IL 6 ) to support the expansion and development of purified human CD34+ bone marrow cells . ^^^ Maximal expansion of nonadherent cells ( 42 fold ) and total CD34+ cells ( 12 . 6 fold ) occurred in PMVEC contact cultures treated with GM CSF + IL 3 + SCF + IL 6 , with similar increases in the number of granulocyte macrophage colony forming units ( CFU GM ) , CFU mix , erythroid burst forming units ( BFU E ) , CFU blast and CFU megakaryocyte ( CFU Mk ) progenitor cells . ^^^ In long term PMVEC contact cultures , CD34+ cells seeded onto PMVEC monolayers with GM CSF + IL 3 + SCF + IL 6 showed a total calculated expansion of over 5 , 000 , 000 fold of nonadherent cells over 35 days in culture . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Between days 5 and 7 of culture , CD34+PKH2bright and CD34+PKH2dim cells were sorted in a limiting dilution scheme into 96 well plates prepared with medium , SCF , IL 3 , IL 6 , granulocyte macrophage colony stimulating factor , and erythropoietin . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In the presence of fetal bovine serum ( FBS ) , IL 12 increased the number of colony forming units for granulocyte macrophage ( CFU GM ) from CD34+33 cells which responded to IL 3 , but not the number of CFU GM from those which responded to stem cell factor ( SCF ) or granulocyte colony stimulating factor ( G CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
When stem cells were stimulated in liquid culture with FL plus interleukin ( IL ) 3 , IL 6 , granulocyte colony stimulating factor ( G CSF ) , or stem cell factor ( SCF ) , cells capable of forming colonies in secondary methylcellulose cultures ( CFU c ) were produced in high numbers . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Diversity and similarity in signaling pathways of granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) , and stem cell factor ( SCF ) in five human factor responsive leukemia cell lines were investigated by immunoblotting to detect tyrosine phosphorylation of intracellular proteins . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Northern blots of LTBMC adherent layers obtained after in vitro treatment with AM 5 revealed a significant mRNA expression for interleukin 1 alpha ( IL 1 alpha ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , macrophage CSF ( M CSF ) and granulocyte CSF ( G CSF ) , in contrast with adherent layers from untreated cultures which only expressed , in detectable levels , M CSF and stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
When employed with cytokines regulating DC development ( tumor necrosis factor [ TNF ] + GM colony stimulating factor [ GM CSF ] ) , SCF increased the size of monocyte ( mono ) and mono DC colonies arising from cord blood CD34+ progenitor cells . ^^^ Most ( approximately 70 % ) of the CFUs were mono DC CFU , and SCF did not alter the proportion of mono DC CFU to mono CFU obtained with TNF + GM CSF alone . ^^^ In long term cultures established with TNF + GM CSF + SCF , high levels of proliferation were prolonged for up to three weeks . ^^^ There was no induction of polymorphonuclear ( PMN ) cells in 2 degree cultures treated with either GM CSF , GM CSF + SCF or GM CSF + granulocyte CSF ( G CSF ) , implying that the DC progenitor being replated was post GM CFU . ^^^ Thus , SCF augments DC growth from progenitor cells without altering the developmental commitment instituted by TNF + GM CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In the presence of optimal concentrations of recombinant interleukin 3 ( IL 3 ) , stem cell factor ( SCF ) , granulocyte / macrophage colony stimulating factor ( GM CSF ) , and erythropoietin ( Epo ) , low and high doses of RA induced striking differential effects on CD34+ cell proliferation in liquid cultures and colony formation in semisolid assays . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Binding of the cytokines interleukin ( IL ) 1 alpha , IL 3 , IL 6 , stem cell factor ( SCF ) , macrophage inhibitory protein ( MIP ) 1 alpha , granulocyte colony stimulating factor ( G CSF ) , and granulocyte macrophage ( GM ) CSF to these CD34+ cell populations was also analyzed by flow cytometry . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
IL 4 , but not SCF , enhanced ionomycin induced transcription and secretion of several genes , including the cytokines IL 3 , IL 4 , granulocyte / macrophage colony stimulating factor , IL 8 and the receptor for IL 6 in the human HMC 1 mast cell line . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In vitro , SCF synergizes with other growth factors , such as granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor , and interleukin 3 to stimulate the proliferation and differentiation of cells of the lymphoid , myeloid , erythroid , and megakaryocytic lineages . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Ex vivo expansion of peripheral blood mononuclear cells ( MNCs ) , cultured both directly and after selection for CD34+ cells , was compared in static and continuously perfused cultures containing interleukin ( IL ) 3 , IL 6 , granulocyte colony stimulating factor ( G CSF ) , and stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The combination of bFGF or a FGF and G CSF , GM CSF , interleukin 3 , or stem cell factor ( SCF ) had a synergistic effect on blast colony formation in three patients . ^^^ In fact , bFGF was found to suppress the stimulation of blast colony formation due to GM CSF in one of 10 patients and that due to SCF in four of eight patients . aFGF suppressed the stimulation of blast colony formation due to GM CSF in two of 11 patients and that due to SCF in four of eight patients . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
TGF beta 1 at 80 pM exhibited differential effects on the normal hematopoietic progenitors when cells were stimulated with different growth factors , such as G CSF , GM CSF , interleukin 3 ( IL 3 ) , or stem cell factor ( SCF ) . ^^^ The suppressive effect by TGF beta 1 was increased for growth with GM CSF , IL 3 , and SCF , and growth with G CSF was unaffected in hematologic malignancies , TGF beta 1 suppression for growth with G CSF was increased for essential thrombocythemia ( ET ) and polycythemia vera ; chronic myelogenous leukemia ( CML ) in chronic phase ; CML in accelerated phase ; CML in myeloid crisis ; myelodysplastic syndrome ( MDS ) in refractory anemia ; MDS in refractory anemia with an excess of blasts ; and acute myeloblastic leukemia ( AML ) . ^^^ The mean ED50s for the growth of leukemic blast progenitors were 1 . 6 , 1 . 2 , 0 . 7 , and 0 . 2 pM in the presence of G CSF , GM CSF , IL 3 , and SCF , respectively , c myc and c myb antisense oligonucleotides significantly suppressed the growth of leukemic blast progenitors , but not that of clonogenic cells from normal donors and patients with ET . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Leukocytes from 12 patients with documented anaphylactic sensitivity to wasp venom were preincubated in the presence or absence of IL 3 , granulocyte / macrophage colony stimulating factor ( GM CSF ) , IL 5 , IL 8 , or stem cell factor ( SCF ) . ^^^ Preincubation of leukocytes with IL 3 , GM CSF , or IL 5 ( 0 . 02 2 ng / ml ) , but not with IL 8 and SCF , caused a dose dependent enhancement of antigen induced basophilic histamine release in all subjects tested . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The same RA patient also showed a marked increase in myeloid colony numbers , which were undetectable with granulocyte macrophage colony stimulating factor alone , but within the normal range when SCF was added . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
While interleukin ( IL ) 3 and IL 5 ( 20 ng / ml ) were unable to modulate immunologic HR , GM CSF ( 20 ng / ml ) produced significant potentiation ( P < 0 . 05 ) , which was , however , smaller than that observed with SCF . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The exogenously added GFs interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , stem cell factor ( SCF ) , and erythropoietin ( Epo ) each exhibited different , but reproducible , consumption kinetics . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The FLT 3 gene encodes a receptor tyrosine kinase that is closely related to two well known receptors , KIT and FMS , that regulate with their respective ligands , stem cell factor ( SCF ) and macrophage colony stimulating factor ( M CSF ) , proliferation and differentiation of hematopoietic cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We describe a subclass of agar based high proliferative potential colony forming cells ( HPP CFC ) derived from populations enriched for early hematopoietic progenitors which , when stimulated with the combination of CSF 1 , G CSF , GM CSF , IL 1 alpha , IL 3 and SCF , produces colonies with trilineage potential for macrophages , granulocytes , and megakaryocytes . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Macrophage colony stimulating factor ( M CSF ) , interleukin 3 ( IL 3 ) , PIXY 321 , stem cell factor ( SCF ) , IL 1 , IL 6 , and IL 11 represent cytokines not yet approved ; the majority of these newer agents have their principal action at an earlier time point in the hematopoietic cascade than the currently approved cytokines . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Neither SCF , IL 3 , GM CSF nor TNF alpha were found in conditioned media of long term bone marrow cultures ( LTBMC ) of normal or MAIDS mice . ^^^ Addition of soluble GM CSF and SCF only transiently increased the production of CFUs ( BFUE and CFU G / M ) in MAIDS LTBMC . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The effect of basic fibroblast growth factor ( bFGF ) alone and in combination with other hematopoietic growth factors on the colony formation of K 562 human leukemic cells was studied using soft agar colony assay . bFGF was found to have a weak colony stimulating activity on K 562 cells derived from the blastic crisis cells of human chronic myelogenous leukemia and to potentiate the K 562 cell colony stimulating activity of granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) and stem cell factor ( SCF ) at a low concentration of below 1 ng / ml . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
This HFFF layer stimulated blood stem cells of GM CSF treated patients significantly more than a cocktail of exogenous growth factors with IL 1 , IL 6 , and stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The present study is to evaluate , in addition to TNF alpha and G CSF , whether serum stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) and granulocyte macrophage CSF ( GM CSF ) levels to see if they were also elevated in patients with acute bacterial infection . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The optimum growth factor combination of conditioned medium from Mia PaCa 2 cells ( Mia CM ) , recombinant granulocyte macrophage colony stimulating factor and recombinant stem cell factor ( SCF ) supported the formation of macroscopic ( > or = mm ) colonies ( 97 % of which were multilineage ) , at an average incidence of 250 / 10 ( 5 ) MNC . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Further , Northern analysis showed that the preadipocyte cell line acquired the ability to produce a significant amount of stem cell factor ( SCF ) , interleukin 6 ( IL 6 ) , leukemia inhibitory factor , and macrophage colony stimulating factor mRNAs in response to IL 1 or lipopolysaccharide stimulation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In vivo daily administration of SCF plus GM CSF results in a synergistic increase in marrow neutrophils , but not the striking synergistic increase in circulating neutrophils that is observed after SCF plus G CSF . ^^^ Colony forming assays reveal a synergistic increase in CFU GMs in the marrow , but not in peripheral blood , after coincubation with SCF plus GM CSF as opposed to GM CSF alone , demonstrating anatomic compartmentalization between a more primitive marrow CFU GM subset and a more mature peripheral blood CFU GM subset . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
SCF at a determined optimum concentration of 100 ng / mL added to the combination of IL 3 , GM CSF , and G CSF enhanced colony growth and size relative to proliferation in response to the latter three factors alone ( from 78 to 188 total colonies / 10 ( 4 ) PBHP plated and from 10 to 93 large [ > 200 cells ] colonies / 10 ( 4 ) PBHP plated ) . ^^^ Furthermore , addition of bFGF and / or IL 6 to the combination of optimum concentrations of SCF , IL 3 , GM CSF , and G CSF further enhanced colony number and size in a dose dependent fashion . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The effects of interleukin 1 beta ( IL 1 ) , IL 3 , IL 6 , granulocyte macrophage CSF ( GM CSF ) , G CSF , macrophage CSF ( M CSF ) , stem cell factor ( SCF ) , erythropoietin ( EPO ) , leukemia inhibitory factor ( LIF ) , and interferon gamma , as well as 36 combinations of these factors were tested . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
A hierarchy appeared clearly regarding sensitivity to the drug in the following order of increasing resistance : PCM CFU GM ( grown in presence of placenta conditioned medium ) , 4R CFU GM ( grown in presence of GM CSF + IL 3 + G CSF + EPO ) , 5R CFU GM ( grown in presence GM CSF + IL 3 + G CSF + EPO + SCF ) and LTC IC with respective lethal dose 95 ( LD 95 ) levels of 40 micrograms , 55 micrograms , 90 micrograms and 140 micrograms / 10 ( 7 ) MNC ( P = 0 . 05 to P = 0 . 018 ) . ^^^ Even the primitive stem cells treated with very high doses of mafosfamide ( 2 to 4 times the usually recommended dose ) responded to a combination of SCF + GM CSF + G CSF + IL 3 in liquid marrow culture , suggesting that they were functionally spared by the treatment . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
It is particular mechanism that we have investigated , showing that molecules such as GM CSF , G CSF , IL 6 , IL 8 and SCF can be produced by airways epithelial cells and fibroblasts in quantities sufficient to induce hemopoietic events , either systemically or locally . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
All four tumors stained positively for stem cell factor ( SCF ) , granulocyte colony stimulating factor ( G CSF ) , interleukin 2 ( IL 2 ) , IL 4 , IL 5 , interferon alpha ( IFN alpha ) , and insulin like growth factor 1 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
These studies show that culture of DR cells for 5 weeks in a `` stroma free ' ' culture supplemented with a combination of four early acting cytokines ( Interleukin 3 [ IL 3 ] , stem cell factor [ SCF ] , leukemia inhibitory factor [ LIF ] , and granulocyte colony stimulating factor [ G CSF ] ) results in a similar cell expansion as when DR cells are cultured in `` stroma noncontact ' ' cultures supplemented with the same cytokines . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
However , SCF , in combination with interleukin 3 ( IL 3 ) or with `` late acting factors ' ' ( granulocyte colony stimulating factor ( G CSF ) or erythropoietin ( Epo ) ) , generated large numbers of mature cells as well as CFC . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
SCF plays an important role in the GM CSF or IL 3 dependent production of neutrophils and macrophages . ^^^ In vitro colony assay also suggests an increase in sensitivity of GM progenitors to cytokines ( GM CSF , IL 3 , G CSF and / or SCF ) in a patient with juvenile chronic myelogenous leukemia . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In liquid cultures , recombinant human stem cell factor ( SCF ) , in combination with interleukin 1 ( IL 1 ) , IL 3 , IL 6 , granulocyte macrophage colony stimulating factor , or granulocyte colony stimulating factor caused untreated , but not 4HC treated , CD34+lin cells to form CFC . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) acts in synergy with other growth factors such as erythropoietin ( Epo ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , or interleukin 3 ( IL 3 ) , to stimulate the growth of primitive hematopoietic cells . ^^^ Because of the prominent role of CSF in the maintenance of normal erythropoiesis in vivo , we examined the effects of SCF on the Epo inducible human erythroleukemia cell line MB 02 , and characterized the c kit receptor in these cells . ^^^ SCF also enhanced cell proliferation in the presence of either IL 3 or low concentrations of GM CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Treatment of these cells with c myb antisense oligodeoxynucleotides caused downregulation of c myb expression , decreased in the number of marrow CFU F colonies ( approximately 54 % inhibition ) and in the cell number within residual colonies ( approximately 80 % ) , and downregulation of granulocyte / macrophage colony stimulating factor ( GM CSF ) and stem cell factor ( SCF ) mRNA expression . ^^^ Transfection of T98G glioblastoma cells , in which expression of c myb , GM CSF , and SCF mRNAs is undetectable or barely detectable , with a plasmid containing a full length c myb cDNA under the control of the SV 40 promoter induced the expression of biologically active SCF and GM CSF in these cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Combination in marrow cultures of SCF and granulocyte colony stimulating factor ( G CSF ) increased cell numbers more than tenfold in developing granulocytic and blast cell colonies . ^^^ Combination with G CSF enhanced progenitor cell numbers five fold in developing blast colonies , but a majority of these were committed macrophage progenitor cells not able to proliferate further with SCF plus G CSF . ^^^ This process of amplified but abortive formation of progenitor cells was also noted with use of the combination of SCF plus IL 6 but not with combination of SCF with granulocyte macrophage CSF ( GM CSF ) or Multi CSF ( IL 3 ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Ten healthy donors and four patients with Diamond Blackfan anemia ( DBA ) have been investigated for granulocyte macrophage colony stimulating factor ( GM CSF ) and stem cell factor ( SCF ) production by bone marrow enriched fibroblasts ( BMEF ) in a highly sensitive biological assay on growth factor dependent M 07e cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Since either an early block of differentiation in stem cells or their insufficient support by stromal functions could be an underlying factor , levels of stem cell factor ( SCF ) and cytokines have been measured in blood and in supernatants of monocytes after stimulation with granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^ In two of three FA patients , no GM CSF was detectable , and simultaneously SCF was decreased to 8 % and 15 % of normal values . ^^^ The combination of low SCF and GM CSF may be implied in the pathogenesis of marrow aplasia , since comparison with W / Sl mice shows that impairment of the SCF / c kit function alone has different effects . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Addition of SCF ( 50 ng / ml ) to marrow cultures stimulated with the standard mixture of growth factors ( interleukin 3 or IL 3 , granulocyte macrophage colony stimulating factor or GM CSF , and erythropoietin or Epo ) significantly increased the mean ( + / SD ) concentration of mafosfamide inducing 95 % inhibition of CFU GM ( 106 + / 17 versus 130 + / 29 , p < or = . 0005 ) , but not granulocyte / erythroid / macrophage / megakaryocyte CFU ( CFU GEMM ) ( 85 + / 4 versus 90 + / 1 , p < or = . 1 ) and BFU E ( 90 + / 5 versus 92 + / 5 , p < or = . 1 ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Third , we have tested the response to SCF of peripheral blood ( PB ) hemopoietic progenitors collected from patients receiving in vivo long term treatment with granulocyte CSF ( G CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
When added to interleukin 3 ( IL 3 ) and / or granulocyte macrophage colony stimulating factor ( GM CSF ) , SCF has an especially profound effect on the in vitro proliferation of several classes of primitive hematopoietic progenitor cells including the burst forming unit megakaryocyte ( BFU MK ) , the high proliferative potential colony forming cell ( HPP CFC ) and the long term bone marrow culture initiating cell ( LTBMC IC ) . ^^^ The addition of SCF , IL 3 and GM CSF to suspension cultures initiated with CD34+HLA DR cells results in an exponential expansion of the numbers of hematopoietic progenitor cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The addition of granulocyte macrophage colony stimulating factor ( GM CSF ) , IL 6 , stem cell factor ( SCF ) , or leukemia inhibitory factor ( LIF ) to low levels of IL 3 also stimulated megakaryocyte growth , as measured by IIb / IIIa expression . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
SCF stimulated leukemic blast progenitors in a manner almost comparable to or more prominently than that of other CSFs , namely , IL 3 , GM CSF , and G CSF , in 9 patients . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The growth factor combination in the perfusion medium consisted of interleukin 3 ( IL 3 ) , stem cell factor ( SCF ) , GM CSF and erythropoietin ( Epo ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Primitive hematopoietic progenitors were stimulated by early acting stimulatory factors such as SCF , IL 11 , IL 9 and the intermediate late acting growth factors IL 3 and granulocyte macrophage colony stimulating factor ( GM CSF ) , alone and in combination . ^^^ When tested on CD34+ / 4 HC resistant progenitors , IL 11 , IL 9 and SCF increased the number of clonogenic cells responsive to IL 3 and GM CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
LDA11 cells can be induced with lipopolysaccharide ( LPS ) , IL 1 alpha , or interferon gamma ( IFN gamma ) to express mRNA for monocyte chemoattractant protein 1 ( MCP 1 / JE ) , IFN inducible protein 10 ( IP 10 ) , stem cell factor ( SCF ) , and macrophage colony stimulating factor ( M CSF ) but not for IL 1 alpha , IL 3 , or tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Overall , our data indicate that STAT 5 is widely expressed in hematopoietic cells and activated by a number of cytokines , including TPO , GM CSF and EPO , but not by IFN gamma or SCF . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Furthermore , fraxiparin was found to potentiate the stimulating activity of aplastic anaemia serum ( AAS ) but not stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) and erythropoietin ( Epo ) , on MK colony growth in vitro , and to neutralize the inhibitory effect of platelet factor 4 ( PF 4 ) in vitro and in vivo . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We also showed that 5 FU resistant PBSC have a greater capacity for expansion of IL 1 / IL 3 / SCF responsive immature progenitors ( p = 0 . 05 ) , amplification of IL 3 plus GM CSF responsive progenitors ( p = 0 . 01 ) , and production of committed single growth factor responsive ( granulocyte macrophage colony stimulating factor [ GM CSF ] ) precursors ( p = 0 . 01 ) than the untreated PBSC . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
While IL 1 or IL 6 had no proliferative effects as single growth factors , IL 1 , in combination with granulocyte macrophage CSF ( GM CSF ) , IL 3 , CSF 1 , and SCF , but not granulocyte CSF ( G CSF ) , enhanced the cloning frequency of Lin Sca 1+ progenitors three to five fold , whereas IL 6 increased the cloning frequency in response to all four CSFs and SCF two to seven fold . ^^^ Furthermore , the combined action of IL 1 and IL 6 resulted in additive or synergistic enhancement of CSF and SCF stimulated colony formation of Lin Sca 1+ high proliferative potential colony forming cells ( HPP CFCs ) . ^^^ Finally , IL 6 , but not IL 1 , enhanced the number of immature blast cells observed in CSF and SCF stimulated cultures . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We have monitored the serum concentrations of hematopoietic growth factors ( HGFs ; ie , stem cell factor [ SCF ] , leukemia inhibitory factor [ LIF ] , interleukin 3 [ IL 3 ] , IL 6 , IL 8 , and granulocyte colony stimulating factor [ G CSF ] ) in 15 lymphoma / leukemia and 6 ovarian cancer patients undergoing autologous bone marrow ( BM ) or peripheral blood ( PB ) stem cell transplantation ( SCT ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Second , it is demonstrated that there is no granulocyte monocyte colony stimulating factor ( GM CSF ) , CSF 1 , interleukin 3 ( IL 3 ) and possibly stem cell factor ( SCF ) production by the marrow cells during the contact . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We cultured M TAT cells long term ( > 1 year ) in the continuous presence of erythropoietin ( EPO ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , or stem cell factor ( SCF ) . ^^^ These long term cultures are referred to as M TAT / EPO , M TAT / GM CSF , and M TAT / SCF cells , respectively . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Neutralizing antibodies to macrophage colony stimulating factor ( M CSF ) and granulocyte macrophage colony stimulating factor ( GM CSF ) but not antibody to interleukin 3 ( IL 3 ) , or stem cell factor ( SCF ) significantly inhibited the growth of the progenitor cells induced by mesangial cell conditioned media . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The remainder ( interleukins 1 to 11 , G CSF , GM CSF , LIF , SCF , erythropoetin , IFN gamma , RANTES , MIP 1 alpha , FGF , EGF , PDGF , dexamethasone , forskolin , retinoic acid and cyclosporin A ) failed to alter expression . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Enhanced gene expression for TNF alpha , macrophage CSF ( M CSF ) , and stem cell factor ( SCF ) was not detected . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Neither stromal cell co culture nor exposure of factor dependent and factor independent cell lines to GM CSF , IL 3 , SCF , or combinations thereof significantly changed the percentage of cells expressing Ki 67 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
After pre exposition to rhIL 3 , IL 1 beta , rIL 6 , GM CSF and stem cell factor ( SCF ) and cloning with SCF the number of hematopoietic progenitors increased 14 18 fold compared to basal values . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Although these single factors stimulate MTHCs , combinations of these factors with colony stimulating factor 1 ( CSF 1 or M CSF ) or Steel factor ( SLF or SCF ) act synergistically to promote colony formation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The viable 5 FU resistant cells were then cultured in semi solid media in the presence of either single cytokines : TCM 5637 , Granulocyte Macrophage Colony Stimulating Factor ( GM CSF ) , or a combination of cytokines : interleukin 1 ( IL 1 ) IL 1 + IL 3 + 5637 , IL 1 + IL 3 + Stem Cell Factor ( SCF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
This is probably an indirect effect inasmuch as these polypeptides can regulate the stromal cell expression of hemopoietic factors such as macrophage colony stimulating factor ( M CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 6 ( IL 6 ) , and the stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The expression of interleukin ( IL ) 7 , stem cell factor ( SCF ) , IL 1 alpha and granulocyte monocyte colony stimulating factor ( GM CSF ) mRNA was mapped to individual stromal cell types , while the expression of IL 1 alpha and GM CSF , along with interferon ( IFN ) gamma and IL 4 was detected in the lymphoid compartment of fetal day ( Fd ) 14 thymus . ^^^ Purified major histocompatibility complex class II+ cortical epithelial cells strongly expressed IL 7 and SCF , but only limited expression of IL 1 alpha and GM CSF could be detected . ^^^ Fetal mesenchyme , on the other hand , expressed SCF , IL 1 alpha and GM CSF but not IL 7 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In a kinetic analysis , colony formation by PB granulocyte macrophage colony forming unit ( CFU GM ) was delayed in a dose dependent manner by the addition of activin A only when stimulated with interleukin 3 ( IL 3 ) , but not when stimulated with granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , or stem cell factor ( SCF ) plus G CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
This effect is further increased by addition of other cytokines such as GM CSF , IL 3 , IL 4 , Stem cell factor ( SCF ) , while Tumor Necrosis Factor ( TNF ) and Transforming Growth Factor beta 1 ( TGF beta 1 ) seem to exert an inhibitory activity . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Thus , we further determined the presence in the PBMNC conditioned medium ( CM ) of inhibitory cytokines ( macrophage inflammatory protein 1 alpha [ MIP 1 alpha ] , transforming growth factor beta 2 [ TGF beta 2 ] , interferon gamma [ IFN gamma ] , and tumor necrosis factor alpha [ TNF alpha ] ) and stimulatory cytokines ( granulocyte macrophage colony stimulatory factor [ GM CSF ] , interleukin 3 [ IL 3 ] , and stem cell factor [ SCF ] ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Gradient separated bone marrow mononuclear cells ( BMMNC ) from 38 recipients of TCD BMT , 15 recipients of UM BMT , and 23 normal donors ( NLD ) were plated in cultures of semisolid , serum containing medium with the addition of stem cell factor ( SCF ) , erythropoietin ( Epo ) , and granulocyte colony stimulating factor ( G CSF ) or granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Plasma levels of granulocyte colony stimulating factor ( G CSF ) , granulocyte / monocyte colony stimulating factor ( GM CSF ) , interleukin 6 ( IL 6 ) , stem cell factor ( SCF ) and macrophage inhibition factor alpha ( MIP 1 alpha ) during the transplant procedure were similar whether or not accessory cells were given . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We detected erythropoietin ( EPO ) in 11 of 15 , stem cell factor ( SCF ) in 7 of 11 , granulocyte colony stimulating factor ( G CSF ) in 4 of 15 , granulocyte / macrophage colony stimulating factor ( GM CSF ) in 6 of 15 , IL 3 in 1 of 12 , leukemia inhibitory factor ( LIF ) in 1 of 9 , and macrophage colony stimulating factor ( M CSF ) in 1 of 8 conditioned media . ^^^ With immunoenzymatic labeling we localized EPO and SCF to the cytoplasm of epithelial HB cells , whereas stromal cells and cells of immature fibrous tissue of mixed HB expressed SCF , G CSF , GM CSF , LIF , and M CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Tumour necrosis factor ( TNF ) alpha exerts multiple effects on human acute myeloblastic leukaemia ( AML ) cells in vitro , including ( 1 ) synergistic stimulation of proliferation with interleukin 3 ( IL 3 ) and granulocyte macrophage colony stimulating factor ( GM CSF ) ; ( 2 ) inhibition of granulocyte CSF ( G CSF ) and stem cell factor ( SCF ) induced growth ; ( 3 ) suppression of multiplication of clonogenic leukaemic cells ; ( 4 ) induction of autocrine growth . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Serum levels of stem cell factor ( SCF ) , interleukin ( IL ) 1 alpha , IL 6 , IL 3 , granulocyte macrophage CSF ( GM CSF ) and G CSF were analyzed before chemotherapy and during the myelosuppressive stage and correlations between cytokine levels and myelosuppression severity were examined . ^^^ Serum SCF levels did not fluctuate significantly during myelosuppression , and IL 1 , IL 3 and GM CSF were rarely detected in serum even after chemotherapy . ^^^ In the present study , the roles of IL 1 alpha , SCF , IL 3 and GM CSF chemotherapy induced myelosuppression were not clear . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We have used a sensitive bioassay to investigate production of granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin ( IL ) 3 , IL 6 and stem cell growth factor ( SCF ) , by normal and AA stroma in long term bone marrow culture ( LTBMC ) . ^^^ All normal stroma populations produced G CSF and GM CSF , 93 % produced IL 3 , 80 % produced IL 6 , and 70 % produced SCF . ^^^ Similarly , all AA stroma produced G CSF and GM CSF , and 71 % produced SCF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Culture of DR cells in a combination of M 2 10B4 derived PG , but not glycoproteins and picogram concentrations of recombinant human interleukin 6 ( IL 6 ) , granulocyte colony stimulating factor ( G CSF ) , stem cell factor ( SCF ) , leukemia inhibitory factor ( LIF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and macrophage inflammatory protein 1alpha ( MIP 1alpha ) resulted in the recovery of 96 % + / 8 % of LTC IC maintained in cultures supplemented with unfractionated stromal SN . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
FL alone was a weak growth stimulator of CD34+ BM cells , but synergistically and directly enhanced colony formation in combination with interleukin ( IL ) 3 , granulocyte colony stimulating factor ( G CSF ) , CSF 1 , granulocyte macrophage ( GM ) CSF stem cell factor ( SCF ) , and IL 6 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
A marked reduction in the number of blast cell colonies supported by SCF alone was noted by the addition of neutralizing antibody ( Ab ) against granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^ SCF caused stimulation by acting synergistically with G CSF , GM CSF , IL 3 , IL 6 , IL 9 , IL 11 , and IL 12 in 20 of 27 ( 74 % ) , 17 of 27 ( 63 % ) , 14 of 28 ( 50 % ) , 9 of 28 ( 32 % ) , 1 of 15 ( 7 % ) , 3 of 28 ( 11 % ) , and 2 of 15 ( 13 % ) patients , respectively . ^^^ G CSF at the optimal concentration increased the sensitivity of blasts to SCF . ^^^ GM CSF , IL 6 , and TNF alpha may be produced endogenously , whereas G CSF and SCF may be supplied exogenously . ^^^ GM CSF , IL 6 , and TNF alpha may be produced endogenously , whereas G CSF and SCF may be supplied exogenously . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The recombinant SCF was similar to natural SCF with respect to its interaction with monoclonal antibody and its synergistic biological activity in conjunction with colony stimulating factor . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
More than moderate levels of RNA for IL 1 alpha were expressed in six of six cell lines , IL 1 beta in four , IL 6 in six , IL 7 in five , IL 10 in six , G CSF in six , GM CSF in six , SCF in six , MIP 2 beta in two , and LIF in six . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) was a weak growth stimulant , however the combination of this factor with GM CSF or IL 3 was synergistically stimulatory . ^^^ These results suggest that leukemic transformation of PMF occurs mainly at the level of myeloid stem cell , and that GM CSF , IL 3 , G CSF and SCF are major growth factors for the blast cells in these cases . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Murine short term bone marrow cultures were also treated with AcSDKP in the presence or absence of cytokines ( interleukin 3 [ LI 3 ] , stem cell factor [ SCF ] , or granulocyte colony stimulating factor [ G CSF ] ) for various time periods . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Constitutively expressed cytokines in this growth phase include G CSF , SCF , LIF , M CSF , IL 6 , and IL 11 , while GM CSF , IL 3 , TGF beta 2 and OSM were not detected in the growth medium . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In contrast , colony and cluster formations from eight of nine c kitR cases was not stimulated at all by SCF alone nor SCF in combination with CSF nor with any CSF alone . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Co incubation of M 07e with various cytokines and TPO showed additive proliferative effects for interleukin 3 ( IL 3 ) and granulocyte macrophage colony stimulating factor ( GM CSF ) and synergistic responses for stem cell factor ( SCF ) , interferon ( IFN ) alpha , and to a lesser extent for IFN gamma and tumor necrosis factor ( TNF ) alpha . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) have been shown to stimulate the circulation of hematopoietic progenitor cells in both mice and nonhuman primates . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
A combination of four growth factors ( GF ) including SCF , IL 3 , IL 6 and GM CSF was identified as the optimal combination for expanding clonogenic progenitor cells in MDS bone marrow liquid cultures . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The FLT3R belongs to a family of receptor tyrosine kinases involved in hematopoiesis that also includes KIT , the receptor for SCF ( stem cell factor ) , and FMS . the receptor for M CSF ( macrophage colony stimulating factor ) . ^^^ GM CSF ( granulocyte macrophage CSF ) , M CSF , interleukin 3 ( IL 3 ) , PIXY 321 ( an IL 3 / GM CSF fusion protein ) and SCF . ^^^ In the mouse , FL potently enhanced growth of various types of progenitor / precursor cells in synergy with G CSF , GM CSF , M CSF , IL 3 , IL 6 , IL 7 , IL 11 , IL 12 and SCF . ^^^ Combinations of FL with G CSF , GM CSF , M CSF , IL 3 , PIXY 321 or SCF and FL with IL 3 or IL 7 had synergistic or additive mitogenic effects on primary AML and ALL cells , respectively . ^^^ The potent stimulation of the myelomonocytic cell lines was further augmented by addition of bFGF ( basic fibroblast growth factor ) , GM CSF , IL 3 or SCF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The combinations of cytokines tested that were most efficient in inducing the CD34+ cells into cycle were stem cell factor ( SCF ) plus one of the following : interleukin 1 ( IL 1 ) , IL 3 , granulocyte colony stimulating factor ( G CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The cytokines interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) and stem cell factor ( SCF ) could independently enhance the FL stimulated proliferation in a synergistic fashion . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Culturing sorted CD34+ LFA 1+ cells in single cell per well assays in medium supplemented with SCF , Epo , IL 3 , Il 6 , GM CSF , and G CSF revealed that they gave rise to dispersed macrophage like colonies , supporting the notion that CD34+LFA 1+ cells indeed consist of a mature committed cell population . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Interestingly , none of them secreted GM CSF , SCF or IL 3 , which are cytokines reputed for their ability to stimulate hematopoietic progenitors , and strikingly , only two of them were able to produce detectable levels of G CSF in culture supernatants despite the propensity of all of them to favor granulocyte differentiation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We also show that heparin significantly potentiates the megakaryocytopoietic activity of C Mpl ligand and interleukin ( IL ) 6 but not IL 3 , GM CSF , SCF , and Epo . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Also , none of these compounds affected SCF synergism with other hematopoietic growth factors , such as interleukin 3 or granulocyte colony stimulating factor , suggesting a population specific phenomenon . ^^^ Stem cell factor ( SCF ) , a potent comitogen for many hematopoietic growth factors , also synergized with GM CSF . ^^^ However , the synergistic enhancement of GM CSF with xanthine / XO and SCF was not additive , indicating that xanthine / XO and SCF may target the same subpopulation of HPC . ^^^ Support for this conclusion came from experiments demonstrating that 1 ) mutant mice strains constitutively lacking a SCF responsive population of HPC [ White spotted ( W / WV ) and Steel ( SI / SId ) ] are unresponsive to xanthine / XO and SCF induced enhancement of GM CSF and 2 ) 3 , 4 epoxybutene , which selectively abrogates SCF synergy with GM CSF , inhibits xanthine / XO induced enhancement . ^^^ Both N tert butyl alpha phenylnitrone and superoxide dismutase effectively inhibited SCF and xanthine / XO synergism with GM CSF , whereas catalase had no effect , indicating that the superoxide anion may be involved . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) and transforming growth factor beta 1 ( TGF beta l ) potently inhibited the clonal growth of murine Lin Sca l+ bone marrow progenitors stimulated by FL alone or in combination with granulocyte colony stimulating factor ( G CSF ) , stem cell factor ( SCF ) , interleukin ( IL ) 3 , IL 6 , IL 11 , or IL 12 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In addition , individual cells in this population can differentiate either to natural killer cells in the presence of stem cell factor ( SCF ) , interleukin 7 ( IL 7 ) , and IL 2 or to dendritic cells in the presence of SCF , granulocyte macrophage colony stimulating factor , and tumor necrosis factor alpha ( TNFalpha ) , indicating that CD34+CD38dim thymocytes contain multi potential hematopoietic progenitors . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In contrast , stem cell factor ( SCF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and interleukin 3 ( IL 3 ) , as well as G CSF , when tested individually , generated significant numbers of GM colonies in some JCML patients . ^^^ In particular , GM CSF plus SCF exerted an interaction equivalent to the all factor combination in most patients . ^^^ Significant differences in the size and constituent cells of GM colonies stimulated by GM CSF plus SCF were also observed . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Incubation with single recombinant cytokines from a broad spectrum of growth factors established that the strongest proliferation response of MUTZ 2 cells was elicited by FLT 3 ligand , granulocyte colony stimulating factor ( G CSF ) , macrophage CSF ( M CSF ) , interferon gamma ( IFN gamma ) and stem cell factor ( SCF ) , whereas granulocyte macrophage CSF ( GM CSF ) , M CSF , interleukin 3 ( IL 3 ) and SCF were the most effective growth factors in inducing proliferation of MUTZ 3 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
IL 4 , stem cell factor ( SCF ) , tumor necrosis factor ( TNF ) , and granulocyte or granulocyte macrophage colony stimulating factor . hCG concentration was determined by immunoradiometric methods and cell number was enumerated by means of an electronic particle counter . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The purified CD34+ cells were cultured for 14 days with various combinations of cytokines , including recombinant human macrophage colony stimulating factor ( rM CSF ) , granulocyte CSF ( rG CSF ) , granulocyte macrophage CSF ( rGM CSF ) , interleukin 3 ( rIL 3 ) , and stem cell factor ( SCF ; a ligand for c kit ) in serum free medium . ^^^ Evidence we obtained showed that SCF plays a central role in the leukemic type growth of MDS CD34+ cells and that G CSF , GM CSF ; and / or IL 3 synergize with SCF to increase undifferentiated blast cell colonies and clusters over that seen in normal CD34+ cells . ^^^ This depends , at least in part , on cytokines such as G CSF , GM CSF , IL 3 , and SCF . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We have examined the repopulating ability of bone marrow and peripheral blood cells collected immediately and at intervals after treatment of donor mice with the combination of granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Transcripts for interleukin ( IL ) 6 , IL 7 , macrophage colony stimulating factor ( M CSF ) , and stem cell factor ( SCF ) were detected in FS 1 cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
It has been demonstrated that these cells produce RNA corresponding to the interleukins IL 1 alpha , 1 beta , 3 , 7 , 8 , and 11 , granulocyte macrophage colony stimulating factor ( GM CSF ) , stem cell factor ( SCF ) , transforming growth factor beta ( TGF beta ) , tumor necrosis factor alpha ( TNF alpha ) , interferon alpha ( INF alpha ) , and basic fibroblast growth factor ( bFGF ) . ^^^ Additionally , RNA corresponding to the receptors for IL 6 , GM CSF , SCF , INF alpha , beta , bFGF , and monocyte colony stimulating factor ( M CSF ) were also expressed by the cells . ^^^ Analysis at the protein level demonstrated that significant amounts of INF alpha , TNF alpha , GM CSF , SCF , IL 1 alpha , and a soluble form of the IL 6 receptor were produced by the cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
MTK 1 also inhibited the bone marrow derived colony forming unit granulocyte / macrophage ( CFU GM ) formed by granulocyte macrophage colony stimulating factor ( GM CSF ) and SCF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
TPO significantly enhanced the cytokine induced growth of AML cells in a substantial fraction of cases responsive to GM CSF , IL 3 , IL 6 or SCF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Both preclinical and early clinical studies using recombinant methionyl human SCF plus recombinant methionyl human granulocyte colony stimulating factor ( Filgrastim ) have demonstrated increased PBPC mobilization as compared with the use of either factor alone . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Although c fms is homologous to c kit , which encodes the receptor for stem cell factor ( SCF ) , EML C 1 cells neither express c fms nor respond to macrophage colony stimulating factor ( M CSF ) , the ligand for c fms . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
A number of factors possessing burst promoting activity ( BPA ) have been characterized , such as interleukin 3 ( IL 3 ) , stem cell factor ( SCF ) , and granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Progenitors which responded to IL 3 , GM CSF , G CSF , M CSF and SCF were then assayed in soft agar cultures . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Cells grown in SCF also can be maintained in GM CSF but not vice versa . ^^^ Stem cell factor removal results in apoptosis and a decline in viability which can be restored partially by re addition of SCF , GM CSF , or co culture with adherent marrow stromal cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
AML cells were induced to proliferation by G CSF , GM CSF , IL 3 , IL 1 beta and SCF to a different extent . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Using delayed addition of growth promoting cytokines , we demonstrate that FL potently promotes the in vitro survival of Lin Sca 1+ progenitors responsive to a potent myeloid growth factor combination ( FL , stem cell factor ( SCF ) , granulocyte CSF ( G CSF ) , and IL 1 alpha ) . ^^^ Whereas SCF was superior to FL in promoting the survival of FL , SCF , G CSF , and IL 1 alpha stimulated Lin Sca 1+ progenitors , FL was more efficient than SCF at promoting the survival of progenitors with a B cell potential , as measured by their ability to produce B220+ cells in response to delayed addition of FL , SCF , and IL 7 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
A clinical isolate of HCMV inhibited the colony formation of myeloid BM progenitors responsive to granulocyte macrophage colony stimulating factor ( CSF ) , granulocyte CSF , macrophage CSF , interleukin 3 ( IL 3 ) and the combination of IL 3 and stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We report here that the cytokine combination granulocyte macrophage CSF ( GM CSF ) plus TNF alpha and stem cell factor ( SCF ) commonly used for the in vitro generation of DC in serum / plasma supplemented medium is , in the absence of serum supplementation , very inefficient in inducing DC development . ^^^ Culture of CD34+ cells under serum free conditions with TGF beta 1 plus GM CSF , TNF alpha , and SCF strongly induces DC differentiation . ^^^ This culture condition is even more efficient than culturing CD34+ cells with GM CSF plus TNF alpha and SCF in the presence of cord blood plasma . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Cells from a single blast colony were separated into two aliquots and replated in secondary methylcellulose cultures containing SCF and IL 7 for B cell lineage and SCF , IL 3 , G CSF , GM CSF and EPO for myeloid lineage . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Substantial expansion of myeloid ( 181 fold ) and megakaryocyte ( 41 fold ) progenitors cells was demonstrated , using the conditions that we determined to be optimal : CD34+ progenitors cultured unperturbed for 7 ( marrow ) or 10 ( blood ) days in Teflon coated bags with 10 Vivo 10 medium containing 10 % autologous plasma , 100 ng / ml , respectively , of recombinant stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , interleukin 6 ( IL 6 ) , and granulocyte colony stimulating factor ( G CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In methylcellulose assays rhMIP 1 alpha had no consistent effect on colony / cluster formation in the presence of either GM CSF + SCF or HPCM . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We compared the in vitro response of myeloid progenitor cells [ colony forming units of culture ( CFU C ) ] that were prepared from human umbilical cord blood to the administration of the combination of granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) versus that of CFU C obtained from normal human bone marrow . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We propose that these unique plasma , which do not contain elevated level of IL 6 , IL 3 , GM CSF , IL 1 beta , erythropoietin or SCF , probably contain high levels of TPO . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The ability of Tpo to maintain viable Lin Sca 1+ progenitors was comparable to that of granulocyte colony stimulating factor and interleukin 1 , whereas stem cell factor ( SCF ) promoted the viability of a higher number of Lin Sca 1+ progenitor cells when incubated for 40 hours . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In previous studies we showed that 5 days of treatment with granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) mobilized murine repopulating cells to the peripheral blood ( PB ) and that these cells could be efficiently transduced with retroviral vectors . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Similar results were obtained in culture supplemented with a combination of IL 3 , IL 6 , SCF , granulocyte colony stimulating factor , erythropoietin , and thrombopoietin . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CD34+ cells were cultured in semisolid medium containing serum with increasing concentrations of GM CSF , G CSF , macrophage colony stimulating factor ( M CSF ) , stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) and IL 11 alone or in combination with HGF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
No significant ( < 10 pg / ml ) GM CSF , SCF , LIF , MIP 1 alpha , IL 2 , IL 4 , IL 11 , IL 12 , IL 13 , IL 15 , sIL 2R , or IFN gamma was detected . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CTS cells showed no significant proliferative response to the cytokines , IL 2 , IL 3 , IL 6 , IL 11 , GM CSF , G CSF , EPO , SCF , but were induced to differentiate to the T cell , B cell , erythroid or megakaryocytic lineage in the presence of particular cytokines . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In contrast , stem cell factor ( SCF ) , granulocyte / macrophage colony stimulating factor ( GM CSF ) , IL 10 , IL 8 , monocyte chemotactic and activating factor ( MCAF ) , and the complement split product C5a failed to modulate the expression of any adhesion molecule examined . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
First , CD34+ cells ( 2 MM , 4 NHL ) were grown for 14 days in 5 ml of IMDM plus 12 . 5 % horse serum ( HS ) , 12 . 5 % fetal calf serum ( FCS ) and a commonly used combination of cytokines : IL1alpha , IL 3 , IL 6 , SCF , GM CSF , G CSF ( 10 ng / ml each ) and EP ( 4 UI / ml ) . ^^^ Two cultures ( 1 MM , 1 NHL ) were carried out for 14 days in 20 ml of 10 Vivo 10 medium , 2 % human serum , IL1alpha , IL 3 , IL 6 , SCF , GM CSF , G CSF ( 6 ng / ml each ) and EP ( 2 UI / ml ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Combination of SCF , IL 6 , IL 3 , and GM CSF increases the mitotic index in short term bone marrow cultures from acute promyelocytic leukemia ( APL ) patients . ^^^ This study examined the proliferative ability of the recombinant human growth factors stem cell factor ( SCF ) , interleukin 6 ( IL 6 ) , interleukin 3 ( IL 3 ) , and granulocyte macrophage colony stimulating factor ( GM CSF ) to determine if they would provide a consistent improvement over the standard cytogenetic culturing techniques in terms of mitotic index ( MI ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Male donor mice ( age 8 12 weeks , weight 20 25 grams ) were pretreated intraperitoneally ( ip ) with a fixed dose of 2 . 5 micrograms of either granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , IL 3 , stem cell factor ( SCF ) , or saline administered twice daily for 2 to 4 days . ^^^ Donor mice pretreated with HGF for 2 days and subsequently injected with IL 8 showed an increase in the numbers of circulating CFU GM per mL blood from 168 + / 98 to 402 + / 201 ( mean + / SD , CFU GM / mL blood ) when GM CSF was used , 314 + / 133 to 2502 + / 513 with G CSF , and 27 + / 15 to 524 + / 339 with SCF compared with saline pretreated controls ( 28 + / 17 to 462 + / 335 CFU GM / mL blood , mean + / SD ; n = 42 and 40 per interval ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Mononuclear BM cells from patients with newly diagnosed AML were cultured in serum free conditions with flt 3 L , SCF , granulocyte colony stimulating factor ( G CSF ) and granulocyte macrophage colony stimulating factor ( GM CSF ) alone and in combination . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Bronchial myofibroblasts grown from bronchial biopsies were capable of producing GM CSF , interleukin 6 ( IL 6 ) , interleukin 8 ( IL 8 ) , and stem cell factor ( SCF ) constitutively . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We investigated the recovery of human myeloid progenitors incubated with IL 11 alone or in combination with other cytokines , including stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) and granulocyte macrophage colony stimulating factor ( GM CSF ) following their in vitro treatment with ARA C ( 10 ( 9 ) M ) or Eilatin ( 10 ( 7 ) M ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Among recombinant cytokines , interleukin 3 ( IL 3 ) , IL 6 , and IL 7 , but not IL 2 , IL 4 , stem cell factor ( SCF ) , and granulocyte macrophage colony stimulating factor ( GM CSF ) could induce RAG 1 activation in FL8 . 2 . 4 . 4 cells , and a significant synergistic effect between IL 3 , IL 6 , and IL 7 was observed . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
When bone marrow ( BM ) cells were cultured with GM CSF , IL 3 and SCF in a semisolid system , the colonies were exclusively eosinophilic . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Short term mono DC cultures initiated with GM CSF + tumor necrosis factor ( TNF ) + stem cell factor ( SCF ) exhibited increases in IL 6 level until day 11 ( peak DC growth ) . ^^^ Long term mono DC cultures sustained with GM CSF + TNF + SCF contained the highest IL 6 levels ( 671 pg / ml ) on day 11 . ^^^ Anti IL 6 profoundly inhibited cell proliferation associated with DC hematopoiesis when added on days 0 , 2 and 5 to GM CSF + TNF + SCF cultures , indicating that various stages of mono DC development rely on IL 6 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The addition of granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage CSF ( GM CSF ) , IL 1beta , IL 11 or macrophage inflammatory protein 1alpha to cultures containing IL 3 , SCF and IL 6 could not explain the SCM effect and in all these combinations SCM addition further increased the recovery of HSC subsets . ^^^ Similarly , addition of anti cytokine antibodies ( ie alpha G CSF , alpha GM CSF , alpha IL 11 , alpha leukemia inhibitory factor ) to liquid cultures containing IL 3 , SCF , IL 6 and SCM could not neutralize the SCM effect . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In the present work , mouse spleen cells were cultured with recombinant ( r ) IL 1 beta , rIL 5 , rIL 6 , rIL 9 , granulocyte macrophage colony stimulating factor ( GM CSF ) , stem cell factor ( SCF ) , tumor transforming growth factor beta ( TGF beta ) , or tumor necrosis factor alpha ( TNF alpha ) in the presence of endogenous IL 3 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The effects of recombinant thrombopoietin ( TPO ) alone and in combination with erythropoietin ( EPO ) and early acting cytokines such as interleukin 3 ( IL 3 ) , stem cell factor ( SCF ) and GM CSF on highly purified mobilized human CD34+ progenitor cells were studied in a serum depleted culture system . ^^^ The addition of TPO to early acting cytokines ( EPO , GM CSF , SCF and / or IL 3 ) not only increased the overall megakaryocyte expansion , but also generated a different maturation pattern of the CD41+ megakaryocyte progenitors . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
A combination of erythropoietin ( EPO ) plus stem cell factor ( SCF ) drove purified unfractionated granulocyte colony stimulating factor ( G CSF ) / chemotherapy mobilized peripheral blood CD34+ cells to selective erythroid differentiation in liquid culture with an average 28 fold increase in the total cell number after 21 d . ^^^ In parallel experiments the same circulating CD34+ cells underwent granulocytic / monocytic differentiation in liquid culture in response to granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) and SCF , demonstrating that these CD34+ progenitors had intact pluripotent differentiating potential . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The effect of macrophage colony stimulating factor ( M CSF ) and stem cell factor ( SCF ) on osteoclast progenitors was examined by pre culturing mouse bone marrow ( BM ) cells in agar medium containing M CSF or SCF and overlaying CESJ medium 0 7 days later . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
PV hematopoietic progenitor cells also exhibit increased sensitivity to the hematopoietic growth factors GM CSF , IL 3 , and SCF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
However , mobilized CD34+ cells were significantly more responsive than their steady state BM counterparts to interleukin 3 ( IL 3 ) and stem cell factor ( SCF ) combined with G CSF or IL 3 in presence of erythropoietin ( Epo ) . ^^^ However , longer incubation with Ara C ( 16 to 18 hours ) , in the presence of SCF , IL 3 and G CSF , or IL 6 , showed that more than 60 % of LTC IC are actually cycling , with no difference being found with BM cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We conclude that long term marrow repopulating cells mobilized into circulation by treatment with granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) are capable of maintaining lifelong polyclonal hematopoiesis in reconstituted mice . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The growth factors granulocyte colony stimulating factor ( G CSF ) and erythropoietin ( EPO ) , but not interleukin 3 ( IL 3 ) or stem cell factor ( SCF ) , also stimulated platelet activation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The unstimulated and induced production of granulocyte macrophage colony stimulating factor ( GM CSF ) , granulocyte colony stimulating factor ( G CSF ) , IL 3 , IL 6 , stem cell factor ( SCF ) , IL 1beta , tumour necrosis factor alpha ( TNF alpha ) , TNF beta , interferon gamma ( IFN gamma ) and transforming growth factor beta ( TGF beta ) was determined after culture of blood mononuclear cells from 22 patients with severe beta thalassaemia in a regular transfusion programme , five non regularly transfused patients with beta thalassaemia intermedia and nine normal persons . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In a methylcellulose system , where a combination of stem cell factor ( SCF ) , IL 3 , and granulocyte / macrophage colony stimulating factor ( GM CSF ) were used , TSP inhibited the growth of colony forming unit granulocyte erythroblast , megakaryocyte , and macrophage ( CFU GEMM ) but not CFU GM and burst forming unit erythroblast ( BFU E ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We studied the interaction of interleukin ( IL ) 4 and other burst promoting activity ( BPA ) factors , such as IL 3 , granulocyte / macrophage colony stimulating factor ( GM CSF ) , IL 9 and stem cell factor ( SCF ) , on erythroid burst forming unit ( BFU E ) and erythrocyte containing mixed ( CFU Mix ) colony formation in serum free culture . ^^^ When CD34+c kit ( high ) cells were used as the target , four factors known to have BPA , IL 3 , GM CSF , IL 9 and SCF , could express BPA . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In comparison with the GM CSF treated cells , the receptors for GM CSF and interleukin 3 ( IL 3 ) were down regulated and the receptors for stem cell factor ( SCF ) and TPO were up regulated in the TPO exposed cells . ^^^ A short term proliferation assay showed a stronger response of the TPO cell lines to erythropoietin , GM CSF , IL 3 , PIXY 321 , SCF and TPO than the GM CSF cell lines . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Mice now exist lacking the granulopoietic factors G CSF , GM CSF , M CSF ( CSF 1 ) , SCF , several other factors influencing haemopoiesis ( including erythropoietin , interleukins 5 and 6 ) , combinations of these factors ( GM & M CSF ; G & GM CSF ; G & GM & M CSF ) and several CSF receptor components . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Costimulatory effects of granulocyte colony stimulating factor ( G CSF ) , granulocyte / macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) and stem cell factor ( SCF ) on the generation of lymphokine activated killer ( LAK ) , bacillus Calmette Gurin activated killer ( BAK ) and natural killer ( NK ) cell cytotoxicity were investigated in this assay . ^^^ GM CSF , but not G CSF , IL 3 or SCF , was able to increase NK , BAK and LAK cytotoxicity in a dose dependent manner . ^^^ GM CSF , but not G CSF , IL 3 or SCF , was able to increase NK , BAK and LAK cytotoxicity in a dose dependent manner . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We show that a five factor combination of interleukin 3 ( IL 3 ) , IL 6 , granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage CSF ( GM CSF ) , and stem cell factor ( SCF ) produces a mean increase of 85 % in colony number . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
After positive selection , CD34+ cells from leukapheresis products of 4 MM and 5 NHL patients and CD34+ cells from bone marrow ( BM ) of 3 healthy donors were grown in IMDM plus 12 . 5 % horse serum , 12 . 5 % fetal calf serum , IL 1alpha , IL 3 , IL 6 , SCF , GM CSF , G CSF ( 10 ng / ml each ) , and EP ( 4 UI / ml ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Activation of ToMC by recombinant human ( rh ) SCF or anti IgE resulted in histamine secretion , whereas no effects were seen with rhC5a , rh granulocyte macrophage colony stimulating factor , or rh interleukin 1 through 10 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The expression patterns of high affinity receptors for G CSF , GM CSF , stem cell factor ( SCF ) , and the ligand for flk 2 on CD38hi and CD 38 / dim PB CD34+ cells were determined by binding of 125I labeled ligand and autoradiography . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CD34+ cord blood ( CB ) cells were expanded in stromal cell free long term culture ( LTC ) , in the presence of various combinations of interleukin 3 ( IL 3 ) , stem cell factor ( SCF ) , IL 6 , granulocyte macrophage colony stimulating factor ( GM CSF ) , and anti transforming growth factor beta ( anti TGF beta ) antibody . ^^^ Our results show that ( a ) CD34+ cell expansion is highly variable depending on the cord blood samples studied , ( b ) significant correlations between B 1 CFC and CD 34 + CD 38 and between total CFU and CD34+ cell expansion are observed , ( c ) SCF in combination with IL 3 appears to expand cell subsets that continue to express their CD 34 + CD 38 phenotype and that generate both immature and committed progenitors , and ( d ) the addition of IL 6 , GM CSF , or anti TGF beta does not significantly improve these expansions . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The expansions of nucleated cells , granulocyte macrophage colony stimulating factor ( GM CSF ) responsive committed precursors , IL 1 + IL 3 + SCF + erythropoietin ( EPO ) responsive multilineage progenitors , and 5 fluorouracil ( 5 FU ) resistant quiescent progenitor were 8 fold , 59 fold , 4 . 4 fold , and 2 . 2 fold , respectively . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Quantitation by specific ELISA and by bioassays detected high amounts of G CSF and GM CSF and smaller quantities of IL 1beta , M CSF and SCF in 5637 CM ; the concentration of IL 3 was below the detection level of the ELISA , if present at all . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
While housekeeping genes were expressed normally in BMSC of infected mice , the expression of genes encoding the essential hemopoietins SCF , granulocyte colony stimulating factor , and interleukin 6 was markedly reduced . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Peripheral blood CD34+ cells were cultured in serum free medium with interleukin 3 ( IL 3 ) , stem cell factor ( SCF ) , and various combinations of thrombopoietin ( TPO ) , IL 6 , GM CSF , and / or G CSF . ^^^ The cytokine combination of IL 3 , SCF , TPO , IL 6 , GM CSF and G CSF produced the greatest number of granulocytes and CFU GM . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Addition of SCF ( 50 ng / ml ) to methyl cellulose cultures stimulated with maximal concentrations of G CSF , GM CSF , interleukin 3 and erythropoietin significantly increased the growth ( mean + / SE ) of CFU Mix ( 7 . 7 + / 1 . 7 versus 2 . 4 + / 0 . 6 , p < or = 0 . 0001 ) , BFU E ( 47 + / 10 versus 32 + / 6 , p < or = 0 . 002 ) and CFU GM ( 173 + / 31 versus 112 + / 20 , p < or = 0 . 0001 ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Bone marrow cells from male B6D2F1 / J mice were cultured for up to 28 days in serum free medium in the absence or presence of stem cell factor ( SCF ) , GM CSF or a combination of the two factors . ^^^ Compared to initial seeding levels , the presence of SCF and GM CSF increased total cell numbers 90 fold and GM CFC numbers 42 fold over a 21 28 day culture period . ^^^ Although spleen colony forming unit cells did not increase , they were maintained at initial seeding levels over a 21 day period in the presence of SCF and GM CSF . ^^^ In lethally irradiated mice , survival enhancement and hematologic reconstitution were optimum with cells cultured for only seven days : survival at six months was 100 % with cells cultured in SCF plus GM CSF or SCF alone , compared to 50 % with cells cultured with only GM CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Proliferation of most ( n = 13 17 ) , but not of all cell lines was significantly enhanced by GM CSF , IL 3 , PIXY 321 , SCF and IFN gamma . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Ex vivo expansion cultures were established in Teflon cell culture bags ( American Fluoroseal Corp , Columbia , MD ) in 25 ml defined medium ( Amgen Inc , Thousand Oaks , CA ) containing 100 ng / ml each of stem cell factor ( SCF ) , granulocyte colony stimulating factor ( G CSF ) , and megakaryocyte growth and development factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Whereas secretion of cytokines lacking a transmembrane domain precursor ( IL 1 alpha , IL 1 beta , IL 6 or IL 10 ) is either unaffected or augmented , shedding / secretion of transmembrane domain containing cytokines and cytokine receptors [ TNF alpha , macrophage colony stimulating factor ( M CSF ) , transforming growth factor ( TGF ) alpha , stem cell factor ( SCF ) , TNF R 55 , TNF R 75 , and IL 6R ] was dramatically decreased in the presence of the metalloproteinase inhibitor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
SCF also potentiates the mobilization of hematopoietic progenitor cells into the peripheral blood in response to granulocyte colony stimulating factor ( G CSF ) , with or without chemotherapy . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In methylcellulose cultures , flt3L stimulated formation of hematopoietic colonies only weakly , whereas it had an additive effect when combined with erythropoietin ( Epo ) , stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , interleukin 11 ( IL 11 ) , or granulocyte colony stimulating factor ( G CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Neither , stem cell factor ( SCF ) nor granulocyte colony stimulating factor ( G CSF ) alone supported the growth of SAS 1 cells , but supported their viability for more than 4 days and arrested them in the G0 / G1 phase . ^^^ SCF also enhanced GM CSF or IL 3 induced growth , but other cytokines did not have this synergistic effect . ^^^ SCF also increased the number of colonies formed by GM CSF or IL 3 treatment , while SAS 1 cells did not form colonies in the presence of SCF alone . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Moreover , GM CSF in the presence of SCF and IL 3 potentiates the HPP CFC formation ( i . e . , an increase of 3 . 1 fold compared to the effect induced by SCF+IL 3 ) with strong inhibitory effects on the number and size of megakaryocyte colonies . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Previous reports demonstrating expression of Hyl and Matk in hematopoietic lineages led us to investigate the regulation of Lsk expression in response to stem cell factor ( SCF ) and granulocyte macrophage colony stimulating factor ( GM CSF ) in M07e , a human leukemic cell line . ^^^ Induction of Lsk / Hyl / Matk protein and mRNA was observed after treatment with SCF but not with GM CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Using suitable assay systems , we analyzed cytokines in AGL SCM and found significant increases in stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and IL 6 levels compared with control SCM . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The combinations of GF used sustained either multilineage [ stem cell factor ( SCF ) + interleukin 3 ( IL 3 ) , or erythroid [ SCF + IL 3 + erythropoietin ( Epo ) ] or myeloid [ SCF + IL 3 + granulocyte colony stimulating factor ( G CSF ) ] differentiation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
TPO significantly enhanced the cytokine induced growth of AML cells in a substantial fraction of cases responsive to GM CSF , IL 3 , or SCF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Besides expressing stem cell factor ( SCF ) and macrophage colony stimulating factor ( M CSF ) , PA 6 cells were found by flow cytometry analysis to express high levels of stem cell antigen 1 ( Sca 1 ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The addition of stem cell factor ( SCF ) , but not of IL 6 , IL 11 , granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage ( GM ) CSF , IL 1 , and IL 7 , increased CFC and LTC IC expansion beyond the levels observed with SCM+IL 3 alone . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Using ELISA and RIA , we studied , in the supernatant of cells from CDCL , the time course of interleukins 3 and 6 ( IL 3 , IL 6 ) , stem cell factor ( SCF ) , granulocyte macrophage , granulocyte and macrophage colony stimulating factors ( GM CSF , G CSF and M CSF ) , macrophage inflammatory protein 1alpha ( MIP 1alpha ) and transforming growth factor beta 1 ( TGF beta 1 ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Addition of HGFs increased the median TE 1 . 8 fold with stem cell factor alone and 2 . 6 fold with SCF , interleukin 3 and GM CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The level of cell adhesion was significantly reduced by low concentrations of interleukin 3 ( IL 3 ) ( 2 . 5 to 10 ng / mL ) , whereas stem cell factor ( SCF ) and granulocyte colony stimulating factor ( G CSF ) at these concentrations did not affect adherence of the cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Recipients were injected at 1 2 years of age with rat stem cell factor ( SCF ) and human granulocyte colony stimulating factor ( G CSF ) for 7 days and tested for donor cells in the blood . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Interestingly , the combination of TPO with cytokines known to have burst promoting activity ( BPA ) , including IL 3 , GM CSF , IL 9 , and SCF , increased the number of BFU E and CFU Mix in the presence of Epo . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
To characterize the expression of wt 1 in differentiating hematopoietic progenitors , we isolated and cultured CD34+ progenitor cells from four healthy bone marrow donors with stem cell factor ( SCF ) and granulocyte colony stimulating factor ( G CSF ) to induce differentiation into granulocytes . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The ligand for flt 3 ( FLT3L ) exhibits striking structural homology with stem cell factor ( SCF ) and monocyte colony stimulating factor ( M CSF ) and also acts in synergy with a range of other hematopoietic growth factors ( HGF ) . ^^^ To investigate the basis for the capacity of FLT3L to augment the de novo generation of myeloid progenitors from CD34+CD38 cells , single bone marrow CD34+CD38 cells were sorted into Terasaki wells containing serum free medium supplemented with interleukin 3 ( IL 3 ) , IL 6 , granulocyte colony stimulating factor ( G CSF ) , SCF ( 4 HGF ) + / FLT3L . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Prior to retroviral infections , CD34+ CB cells were stimulated for 60 h in a serum free medium containing a DC differentiation inducing cytokine cocktail : stem cell factor ( SCF ) , granulocyte / macrophage colony stimulating factor ( GM CSF ) , tumor necrosis factor alpha ( TNFalpha ) , and transforming growth factor beta 1 ( TGF beta 1 ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Significantly , soluble growth factors such as granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) or SCF of MS 5 stromal cells ( MS 5 CM ) could replace the requirement of stroma cells for a considerable period . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Using a combination of GM CSF , SCF , flk 2 / flt 3 ligand , and IL 4 , dendritic cells ( DC ) have been generated in vitro from the adherent fraction of mononuclear cells isolated from the blood of patients with MM . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Low proliferative capacity was observed after 1 week in culture in the presence of stem cell factor ( SCF ) + Flt 3 ligand ( FL ) + G CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Here we examined the effect of combining stem cell factor ( SCF ) and granulocyte colony stimulating factor ( G CSF ) versus G CSF alone in a randomized clinical study involving 62 women with early stage breast cancer . ^^^ As with administration of G CSF alone , the combination of SCF plus G CSF did not alter the wide variation in levels of PBPC observed between individuals and did not alter the selective nature of PBPC release , with preferential release of day 14 granulocyte macrophage colony stimulating factor ( GM CFC ) versus day 7 GM CFC . ^^^ The action of SCF was not explicable in terms of altered SCF , GM CSF , or G CSF responsiveness , but SCF pretreatment was associated with maximum serum SCF levels at the time G CSF was commenced . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Migration depended on the continuous presence of hematopoietic cytokines interleukin 3 ( IL 3 ) , granulocyte colony stimulating factor ( G CSF ) , macrophage CSF ( M CSF ) , granulocyte macrophage CSF ( GM CSF ) , or stem cell factor ( SCF ) , whereas other cytokines , such as IL 8 , macrophage inflammatory protein 1alpha , macrophage chemotactic and activating factor , and erythropoietin resulted in very little or no migratory response . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
An increase in IL 3 + GM CSF + SCF responsive colony forming unit ( CFU ) ( 1 . 7 10 10 ( 4 ) + / 9 . 4 10 10 ( 3 ) and 1 . 6 10 10 ( 5 ) + / 7 . 7 10 10 ( 4 ) respectively ) was also observed as compared with input values ( 1 . 5 10 10 ( 4 ) + / 1 10 10 ( 4 ) and 2 . 3 10 10 ( 4 ) + / 8 . 9 10 10 ( 3 ) respectively ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Results obtained with SCF treatment were similar to those previously obtained in the same model with recombinant human granulocyte colony stimulating factor ( rhG CSF ) treatment except that recovery of lymphocytes to 500 / mm3 appeared to be more rapid in G CSF treated dogs ( median 15 days vs . 21 days , p = 0 . 03 ) . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
A million of lung mast cells generated 41 . 4 pg ( 7 . 0 273 . 6 ) ( median with range ) of TNF alpha , 252 . 6 pg ( 158 . 7 3 , 652 ) of GM CSF and 735 pg ( < 10 2 , 750 ) of IL 5 24 h after activation with SCF and anti IgE . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Our previous studies indicated that maximal production of nucleated cells and myeloid progenitor cells from PB CD34+ cells occurred with multiple hematopoietic growth factor ( HGF ) , notably the 6 HGF combination of interleukin ( IL ) 1 , IL 3 , IL 6 , granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage CSF ( GM CSF ) , and stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
WTKit cells were able to grow in the presence of the human ligand for Kit , stem cell factor ( SCF ) , but displayed reduced growth and clonogenic potential in either SCF or GM CSF compared with the parental cells in GM CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Non adherent mononuclear bone marrow cells were incubated in the presence of the serum and granulocyte / macrophage colony stimulating factor ( GM CSF ) , granulocyte stimulating factor ( G CSF ) , or stem cell factor ( SCF ) , and the number of GM CFU counted . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We report the generation of dendritic cells ( DC ) starting from CD34+ bone marrow ( BM ) progenitor cells , using a two stage culture system in which , besides granulocyte macrophage colony stimulating factor ( GM CSF ) and tumour necrosis factor alpha ( TNF alpha ) , stem cell factor ( SCF ) was added during the first 5 days , while interleukin 4 ( IL 4 ) and / or interferon gamma ( IFN gamma ) were added during the secondary culture period of 9 days . ^^^ We conclude first , that the expansion of DC from BM progenitors could most effectively be enhanced in a two stage culture assay using SCF , GM CSF , TNF alpha and IL 4 ; second , that the effect of TNF alpha in DC generation involves signalling via the TNF R 1 receptor ; and third , that IFN gamma counteracts some of the effects of IL 4 in DC generation . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Moreover , colony formation ( including CFU G , BFU E , CFU Mix , and CFU Meg ) was observed even in the presence of neutralizing antibodies for granulocyte colony stimulating factor , erythropoietin , and thrombopoietin ( c Mpl ) , whereas neutralizing antibodies for gp 130 , IL 6R , IL 3 , and SCF partially or completely blocked the synergistic effect . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Upon culture with granulocyte macrophage colony stimulating factor ( GM CSF ) and stem cell factor ( SCF ) for 14 days , wild type mouse Lin c kit+ HPCs did not exhibit characteristic features of DC such as sheet like projections and veil processes . ^^^ Collectively , these results suggest that TNFalpha has a pivotal role in DC generation from murine early HPCs in collaboration with GM CSF and SCF through the interaction of TNF Rp 55 and TNF Rp75 . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We observed that the best yielding in CD14+ cells was obtained by adding SCF and GM CSF into RPMI 1640 completed medium and by using Teflon bags as culture containers . ^^^ For DC the best association of growth factors combined GM CSF , IL 4 and SCF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
HPC were expanded in the presence of interleukin ( IL ) 3 , IL 6 and stem cell factor ( SCF ) ( days 1 7 ) and subsequently induced by IL 4+ GM CSF ( days 8 26 ) to differentiate to Langerhans type cells ( pLC ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Erythropoiesis was induced in HU 3 parental cells and five clones harvested from culture medium containing GM CSF by replacement of the growth promoting cytokine with stem cell factor ( SCF ) and erythropoietin ( Epo ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
METHODS : Bone marrow cells were grown in stroma free liquid cultures in the presence of SCF , IL 3 , IL 6 and GM CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Transduction was performed with fetal bovine serum ( FBS ) or autologous plasma , IL 3 , GM CSF , IL 6 , and SCF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
To analyze the synergism between SCF and granulocyte macrophage colony stimulating factor ( GM CSF ) , we established a new megakaryoblastic cell line , HML 2 , by culture in the presence of both SCF and GM CSF . ^^^ While SCF alone or GM CSF alone supported modest cell growth , SCF and GM CSF together induced substantial growth of this cell line . ^^^ The combination of SCF and GM CSF resulted in a synergistic increase in tyrosine phosphorylation of the 145 kDa subunit of c kit . ^^^ Thus , a synergistic increase in tyrosine phosphorylation of the 145 kDa subunit of c kit may be one possible mechanism underlying the cooperation of SCF and GM CSF on the HML 2 cell growth . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The response of human acute myelogenous leukemia ( AML ) cells to four different hematopoietic growth factors ( granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 1 beta ( IL 1beta ) , interleukin 3 ( IL 3 ) , and stem cell factor ( SCF ) ) and the relationship of the proliferative response of the AML cells to treatment outcome were studied . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Cells from all three sources that had been expanded ex vivo in the presence of stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , IL 6 , and granulocyte colony stimulating factor ( G CSF ) showed transduction efficiencies ranging from 5 45 % , as measured by acquisition of G 418 resistance . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CD34+ cells were cultured in the presence of EPO , SCF , interleukin 3 , granulocyte / monocyte colony stimulating factor and granulocyte colony stimulating factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Fused sIL 6R / IL 6 produced a marked stimulation of cell expansion and a marked increase in the number of colony forming units when subsequently plated in semi solid medium with IL 3 , GM CSF , SCF and erythropoietin . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
ELISA documented high amounts of granulocyte colony stimulating factor ( G CSF ; > 5 ng / ml ) ; also granulocyte macrophage CSF ( GM CSF ) , macrophage CSF ( M CSF ) , stem cell factor ( SCF ) , IL 6 , and IL 8 were detected in KU 19 19 CM . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
RESULTS : All of these cell lines express granulocyte macrophage colony stimulating factor ( GM CSF ) and macrophage colony stimulating factor ( M CSF ) , and the DU 145 and PC 3 lines express stem cell factor ( SCF ) , as determined by RT PCR and ELISA . ^^^ Each of these cell lines expresses the receptors for SCF , GM CSF , M CSF , and granulocyte colony stimulating factor ( G CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Ligand induced activation of HRMCs through immunoglobulin ( Ig ) E R or SCF R ( c kit ) resulted in histamine secretion ( control : < 10 % ; alphaIgE , 1 microg / mL : 50 . 12 + / 5 . 18 % ; rhSCF , 100 ng / mL : 29 . 24 + / 22 . 39 ) , whereas recombinant C5a , erythropoietin ( EPO ) , IL 1 through 10 , and GM CSF exerted no effects . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We have compared the effects of AcSDKP , Thymosin beta 4 ( Tbeta 4 ) , MIP1alpha and TGFbeta on acute myeloid leukemia ( AML ) and B lineage acute lymphoid leukemia ( B ALL ) cells using liquid cultures in the presence of GM CSF , IL 3 and SCF for AML cells and IL 3 and IL 7 for ALL cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In contrast , marked differences were not found in the levels of interleukin 3 ( IL 3 ) , tumor necrosis factor alpha ( TNF alpha ) , stem cell factor ( SCF ) , granulocyte / macrophage colony stimulating factor ( GM CSF ) , or interferon gamma ) ( IF gamma ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The expansion of bone marrow progenitor cells in a ' stroma free ' long term liquid culture system supplemented with SCF , IL 3 , IL 6 and GM CSF from 19 transplanted patients was significantly reduced compared to normal controls . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We investigated the increase in total nucleated cells , colony forming cells ( CFC ) , CD34+ cells and LTC IC by limiting dilution after a 14 day culture of CB CD34+ cells ( 5 10 10 ( 3 ) / ml ) with SCF , IL 3 , IL 6 , GM CSF and G CSF all at 10 ng / ml . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Therefore , four combinations of SCF , Flt 3 L , IL 3 , erythropoietin and GM CSF as well as three media were evaluated for their capacity to amplify hematopoietic progenitors . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
IL 1 alpha , IL 1 beta , and IL 3 , tumour necrosis factor alpha ( TNF alpha ) , Stem cell factor ( SCF ) and granulocyte macrophage colony stimulating factor ( GM CSF ) ( at 10 ng / ml ) stimulated IL 6 and IL 8 production in 0 % and 10 % FCS . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In order to investigate the sensitivity of malignant target cells to lysis by LAK cells according to their clonogenic capacities and their position along the maturation pathway , we compared clonogenic and chromium release cytotoxicity assays performed on human hematopoietic cell lines using Effector : Target ratios of 1 : 1 , 3 : 1 , 6 : 1 , 12 : 1 , 24 : 1 , 48 : 1 and 96 : 1 , and studied the sensitivity of HL 60 and U 937 human cell lines after exposure to different factors including GM CSF , SCF , IFN , Retinoic acid ( RA ) , DMSO , and TPA which are able to recruit cells into the cell cycle or to induce cell differentiation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In the absence of recombinant human ( rh ) SCF , anti IgE induced a median GM CSF response of 202 ( < 15 to approximately 681 ) pg / 10 ( 6 ) mast cells / 24 h , whereas in the presence of rhSCF the median IgE dependent GM CSF release was 356 ( 152 to approximately 1216 ) pg / 10 ( 6 ) mast cells / 24 h . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CD34+ cells were stimulated with basic fibroblast growth factor ( bFGF ) , stem cell factor / c kit ligand ( SCF ) , interleukin 3 ( IL 3 ) , IL 6 , leukemia inhibitory factor ( LIF ) , and granulocyte monocyte colony stimulating factor ( GM CSF ) alone or in combination in short term serum free liquid suspension cultures ( LSC ) immediately after irradiation and then assayed for clonogenic progenitors . ^^^ Combination of IL 3 with IL 6 , SCF or GM CSF resulted in best survival for CFU GM BFU E and CFU Mix , respectively . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We used the competitive repopulation assay in mice to test the ability of granulocyte macrophage colony stimulating factor ( GM CSF ) and stem cell factor ( SCF ) , cytokines given in clinical settings to enhance marrow recovery after XRT or BMT and to protect the marrow repopulating ability of primitive hematopoietic stem cells ( PHSC ) after these modalities . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Mice were treated with every combination of recombinant human ( rh ) erythropoietin ( EPO ) , rh granulocyte colony stimulating factor ( G CSF ) , recombinant rat ( rr ) stem cell factor ( SCF ) , and rh interleukin ( IL ) 11 by continuous infusion over 7 days ( full factorial design with three dose levels for each cytokine ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
TGF beta 1 was found to be an absolute requirement for in vitro LC development under serum free conditions upon stimulation with the classical DC growth and differentiation factors GM CSF , TNF alpha and SCF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The addition of stem cell factor ( SCF ) and granulocyte colony stimulating factor ( G CSF ) highly enhanced the production of CD19+ cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We previously reported the aberrant growth of granulocyte macrophage ( GM ) progenitors induced by a combination of stem cell factor ( SCF ) and granulocyte macrophage colony stimulating factor ( GM CSF ) in juvenile chronic myelogenous leukemia ( JCML ) . ^^^ In serum deprived single cell cultures of normal bone marrow ( BM ) CD34+CD38high cells , the addition of TPO to the culture containing SCF + GM CSF resulted in an increase in the number and size of GM colonies . ^^^ In the JCML cultures , in contrast , the number of SCF + GM CSF dependent GM colonies was not increased by the addition of TPO . ^^^ The addition of TPO to the culture containing SCF or SCF + GM CSF caused a significant increase in the production of GM colony forming cells by JCML CD34+CD38neg / low population , indicating the stimulatory effects of TPO on JCML primitive hematopoietic progenitors . ^^^ Normal BM cells yielded a significant number of megakaryocytes as well as myeloid cells in response to a combination of SCF , GM CSF , and / or TPO . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We have investigated the growth and proliferation of purified populations of hematopoietic progenitor cells isolated from Nf 1 knockout mice in response to the cytokines interleukin ( IL ) 3 and stem cell factor ( SCF ) , as well as to GM CSF . ^^^ We found abnormal proliferation of both immature and lineage restricted progenitor populations , and we observed increased synergy between SCF and either IL 3 or GM CSF in Nf 1 / progenitors . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Bone marrow mononuclear cells were cultured with combination of human recombinant ( rhu ) erythropoietin ( EPO ) , granulocyte monocyte colony stimulating factor ( GM CSF ) , granulocyte colony stimulating factor ( G CSF ) , Interleukin 3 ( IL 3 ) , either with or without stem cell factor ( SCF ) , and burst forming unit of erythroid ( BFU E ) growth was assessed . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Optimum colony growth was obtained when stem cell factor ( SCF ) and granulocyte macrophage colony stimulating factor ( GM CSF ) were used as growth stimuli of colony forming units ( CFU ) . ^^^ Loss of long term culture initiating ability could be demonstrated in this system with lineage negative marrow cells expanded ex vivo with SCF and GM CSF . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Then CD34+ cells isolated from cord blood were infected three times after a 48 h prestimulation with IL 3 , IL 6 , SCF or with IL 3 , IL 6 , SCF , GM CSF , Flt 3 L and TPO . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We analyzed the effect of tumor necrosis factor ( TNF ) alpha on the differentiation and viability of dendritic cells ( DC ) generated from cord blood CD34+ progenitors cultured for five days with GM CSF , Flt 3 ligand ( FL ) , and stem cell factor ( SCF ) , and then with GM CSF only [ TNF ( ) cultures ] . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We have compared the effects of ATRA and GCSF on the colony growth of 10 MDS marrows , in semi solid and liquid serum free mononuclear cell ( MNC ) cultures , supplemented with a mixture of stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) and granulocyte monocyte colony stimulating factor ( GmCSF ) ( SIGm mix ) , which is fully supportive for myeloid and erythroid ( with erythropoietin ( EPO ) ) colony formation in normal marrow . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Hematopoietic growth factors , such as granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) or stem cell factor ( SCF ) , have been revealed to stimulate the growth of leukemic blast progenitors . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
BACKGROUND : To explore the effect of cytokine therapy on the NADPH oxidase in mature myeloid cells , we isolated neutrophils from patients receiving recombinant human granulocyte colony stimulating factor ( G CSF ) and recombinant human stem cell factor ( SCF ) and evaluated oxidase activity . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Cells with a dendritic morphology and expressing HLA DR , CD1a , S 100 and CD 83 were maximally expanded under serum free conditions with the addition of SCF , GM CSF , TNF alpha , TGF beta and Flt 3 ligand ( fold increase of CD1a+ cells = 102 + / 32 after 2 weeks of culture ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Conversely , production of GM CSF , SCF , M CSF and MCP 1alpha was elevated in NS compared with BM fibroblasts . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We have recently established the culture system to generate dendritic cells ( DCs ) from murine Lin c kit+ bone marrow hematopoietic progenitor cells ( HPCs ) in the presence of granulocyte macrophage colony stimulating factor ( GM CSF ) + stem cell factor ( SCF ) + tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
METHODS : a ) CFU GM , CFU GEMM and BFU E cultures in the presence of CSF , BM serum or HUCB serum ; b ) 35 day LTC in liquid media whether in presence of IL 3 + GM CSF + SCF or autologous serum ( AS ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Granulocyte macrophage colony stimulating factor or stem cell factor ( SCF ) alone could stimulate proliferation of HML / SE cells , however interleukin 3 , interleukin 6 , granulocyte colony stimulating factor and thrombopoietin could not . ^^^ Granulocyte macrophage colony stimulating factor or stem cell factor ( SCF ) alone could stimulate proliferation of HML / SE cells , however interleukin 3 , interleukin 6 , granulocyte colony stimulating factor and thrombopoietin could not . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Marrow stromal cells were evaluated several months after autologous BMT for their capacity to support both normal hemopoiesis and secrete the main growth factors involved in its control , G CSF , GM CSF , IL 3 and SCF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Among the cytokines , IL 3 , IL 6 , and IL 7 , but not IL 2 , IL 4 , SCF , GM CSF induces RAG activation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Clonogenic assays in methylcellulose showed an increased frequency and proliferation of colony forming unit dendritic cells ( CFU DC ) in circulating CD34+ cells , compared to that of BM CD34+ precursors in response to GM CSF and TNF alpha with or without SCF and FLT 3L . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In this study , we have investigated cell reactivity and proliferation with G CSF , granulocyte macrophage colony stimulating factor ( GM CMF ) , macrophage colony stimulating factor ( M CSF ) , stem cell factor ( SCF ) and thrombopoietin ( TPO ) in cases of AML . ^^^ Proliferative reaction of myelogenous leukemia cells with cytokines G CSF , GM CSF , M CSF , SCF and TPO . ^^^ G CSF , GM CSF , M CSF , SCF and TPO caused proliferation of leukemic cells in 25 % , 58 . 3 % , 8 . 3 % , 21 . 1 % and 0 % of cases , respectively . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The CD 14 dependent and independent dendritic cell ( DC ) pathways are instituted simultaneously when CD 34 ( + ) progenitor cells are treated with granulocyte macrophage colony stimulating factor ( GM CSF ) / tumor necrosis factor ( TNF ) + / stem cell factor ( SCF ) ( GTS ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) / granulocyte colony stimulating factor ( G CSF ) mobilized and CD 34 enriched PB cells were divided into two equal aliquots and transduced with one of two retroviral vectors carrying the neomycin resistance gene ( neo ) for 4 days in the presence of interleukin 3 ( IL 3 ) , IL 6 , and SCF in the first 5 animals , IL 3 / IL 6 / SCF / Flt 3 ligand ( FLT ) in 2 subsequent animals , or IL 3 / IL 6 / SCF / FLT plus an autologous stromal monolayer ( STR ) in the final 2 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Supernatants of IL 1alpha stimulated EC potentiated the effects of an association of IL 1 , IL 3 , IL 6 , LIF , SCF , Flt 3 ligand , TPO , G CSF , GM CSF , M CSF and IL 11 on the proliferation of hematopoietic progenitors suggesting that EC may produce other soluble growth factors potentiating the action of the above set of cytokines . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The abilities of stromal cells to produce hematopoietic growth factors , including G GSF , GM CSF , IL 6 and SCF , are either normal or elevated in the majority of patients . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Furthermore , TPO increased the colony stimulatory effects of G CSF , GM CSF , IL 3 , and stem cell factor ( SCF ) on MDS marrow cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Marrow stromal cells of patients treated by autologous bone marrow transplantation ( ABMT ) for malignancies have been assessed for their ability to secrete granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , stem cell factor ( SCF ) , leukemia inhibitory factor ( LIF ) , interleukin 6 ( IL 6 ) , transforming growth factor beta 1 ( TGFbeta 1 ) and macrophage inflammatory protein 1alpha . ( MIP 1alpha ) . ^^^ Significant differences between patient groups and controls were apparent in baseline production of GM CSF , SCF , MIP 1alpha and TGFbeta 1 . ^^^ The production of TGFbeta 1 , LIF , IL 6 and more particularly SCF were reduced in post transplant patients , while elevated levels of GM CSF and MIP 1alpha were observed in these patients only when the values were corrected for the number of cells growing in the SL . ^^^ These results indicate a prolonged stromal defect in growth factor production following ABMT for the early stage acting cytokines IL 6 , LIF and SCF as well as for G CSF , but not for GM CSF , while the production of the 2 inhibitors shows different pathways . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We investigated the increase in total nucleated cells , colony forming cells ( CFC ) , CD34+ cells and long term culture initiating cells ( LTC IC ) by limiting dilution after a 14 day culture of CB CD34+ cells ( 5 10 10 ( 3 ) / ml ) with SCF , IL 3 , IL 6 , GM CSF and G CSF all at 10 ng / ml . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In this study , normal dogs were given retrovirally transduced bone marrow cells with and without preharvest mobilization by the canine growth factors granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We investigated the co stimulatory activity of 14 cytokines ( interleukin 1 to 8 , IL 10 , IL 13 , INF alpha , TNF alpha , GM CSF and SCF ) on the proliferation of CD 40 activated follicular lymphoma cells in comparison to tonsillar GC B cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
A number of haematopoietic factors are described that increase the burst number : interleukin 3 ( IL 3 ) , stem cell factor ( SCF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , IL 9 , IL 11 , insulin like growth factor 1 , and erythroid potentiating activity ( EPA ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
ELISA confirmed the presence of one or several of the following cytokines in the CM of carcinoma cell lines : GM CSF , G CSF , macrophage CSF ( M CSF ) , stem cell factor ( SCF ) and IL 6 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In addition we examined the in vitro effect of MGDF as well as the combined effect of MGDF and granulocyte colony stimulating factor ( G CSF ) or stem cell factor ( SCF ) on leukemic blast proliferation , recruitment into S phase , induction of programmed cell death and activation of signal transducers and activators of transcription ( STAT ) proteins . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Circulating CD34+ cell populations characterized by a low rate ( up to five ) or high rate ( more than five ) of cell divisions were isolated from 8 d cultures in the presence of stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , granulocyte colony stimulating factor ( G CSF ) , erythropoietin ( EPO ) , Flt 3 ligand and Peg rHu megakaryocyte growth and development factor ( Peg rHuMGDF ) using the fluorescent dye 5 , 6 carboxyfluorescein diacetate succinimidyl ester ( CFDA SE ) and flow cytometric cell sorting . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
When proliferation was enhanced by growth promoting cytokines ( e . g . granulocyte macrophage colony stimulating factor , GM CSF , stem cell factor , SCF , or PIXY 321 ) , some previously TGF beta 1 sensitive cell lines acquired cellular resistance toward TGF beta 1 mediated growth suppression , whereas four other cell lines remained susceptible to TGF beta 1 growth inhibition despite possible counteraction by other cytokines . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CD34+ cells were cultured in serum free medium containing stem cell factor ( SCF ) , GM CSF , and interleukin 3 ( IL 3 ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The cytokines investigated included interleukin ( IL ) 1alpha , IL 1beta , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , IL 7 , IL 8 , IL 10 , IL 13 , TNF alpha , TNF beta , IFN gamma , TGF beta 2 , G CSF , GM CSF and SCF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
When combinations of cytokines were used over 7 days , between 5 % ( FLT 3 ligand [ FLT 3L ] , stem cell factor [ SCF ] , IL 3 , IL 6 , granulocyte colony stimulating factor [ G CSF ] , beta nerve growth factor [ betaNGF ] ) and 22 % ( FLT 3L + HGF ) of the cells remained undivided , between 15 % ( HGF , IL 1 , IL 11 , G CSF ) and 68 % ( SCF + TPO ) of the cells had doubled and between 27 % ( FLT 3L + TPO ) and 70 % ( HGF , IL 1 , IL 11 , G CSF ) died . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We used a porcine microvascular endothelial cell ( PMVEC ) line in conjunction with exogenous interleukin ( IL ) 3 , IL 6 , granulocyte macrophage colony stimulating factor ( GM CSF ) , and stem cell factor ( SCF ) to expand human HSCs that express the CD 34 and Thy 1 antigens but lack lineage associated markers ( CD34+Thy 1+Lin cells ) . ^^^ These data suggest that the proliferation of HSCs in the presence of IL 3 , IL 6 , GM CSF , and SCF without stromal cell support may result in impairment of engraftment capacity , which may be overcome by coculture with PMVECs . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Baboon marrow was harvested after in vivo administration ( priming ) of stem cell factor ( SCF ) and granulocyte colony stimulating factor ( G CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The cell lines produced G CSF ( 1 / 11 cell lines ) , GM CSF ( 2 / 11 ) , SCF ( 2 / 11 ) , M CSF ( 6 / 11 ) . and interleukin 6 ( 9 / 11 ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The interaction between SCF and TPO on the neutrophilic cell generation was greater than the combined effects of SCF with granulocyte colony stimulating factor ( G CSF ) or granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^ The addition of neutralizing antibody against G CSF or GM CSF did not influence the SCF + TPO dependent neutrophilic cell production . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
An increased production of G CSF , GM CSF , IL 8 , IL 6 , IL 1alpha , IL 11 , MIP 1alpha and SCF and no production of LIF , TNF alpha , TPO and IL 3 by 2 Gy irradiated TrHBMEC was observed . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Cryopreserved CB CD 34 ( + ) cells as well as freshly isolated CB CD 34 ( + ) cells cultured with granulocyte macrophage colony stimulating factor ( GM CSF ) / stem cell factor ( SCF ) / tumor necrosis factor alpha ( TNF alpha ) for 14 days gave rise to CD1a+ / CD4 ( + ) / CD11c+ / CD14 ( ) / CD40 ( + ) / CD80 ( + ) / CD83 ( + ) / CD86 ( + ) / HLA DR+ cells with dendritic morphology . ^^^ These results indicate that frozen CB CD 34 ( + ) cells cultured with GM CSF / TNF alpha / SCF gave rise to dendritic cells which were morphologically , phenotypically and functionally similar to DCs derived from fresh CB CD 34 ( + ) cells . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Since cytokines are of crucial significance in this process , we decided to examine the role of stem cell factor ( SCF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) , granulocyte colony stimulating factor ( G CSF ) , and interleukin 1 alpha ( IL 1 alpha ) , known for their stimulatory effects on in vitro hematopoiesis ; and transforming growth factor beta ( TGF beta ) , interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) , known for their inhibitory effect , in the anemia of GCA . ^^^ RESULTS : Consistent constitutive gene expression was detected in the marrow stromal cells of all control samples for SCF , GM CSF , IL 1 alpha , TGF beta and TNF alpha , while only 2 / 6 expressed IFN gamma . ^^^ CONCLUSION : The combination of the defective expression of SCF and GM CSF , cytokines with a known stimulatory effect on in vitro hematopoiesis , may contribute to the pathogenesis of anemia in GCA . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Bone marrow cells cultured for high proliferative potential CFCs in the presence of interleukin ( IL ) 1 , IL 3 , macrophage CSF , and stem cell factor ( SCF ) , or cultured for erythroid burst forming units in the presence of IL 3 , SCF , and erythropoietin , also exhibited enhanced growth in the presence of PGG glucan . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Treating HAS 303 cells with SP increased the intracellular Ca2+ concentration and stimulated production of G CSF , GM CSF , SCF and IL 6 , but not IL 1alpha , IL 1beta and TNF alpha , but did not enhance proliferation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Cultures of sorted cells , supplemented with granulocyte colony stimulating factor ( G CSF ) / granulocyte macrophage colony stimulating factor ( GM CSF ) / IL 1 / IL 3 / IL 6 / stem cell factor ( SCF ) and erythropoietin ( EPO ) indicated that the LFA 1 ( neg ) fraction contained the majority of the colony forming cells ( CFCs ) ( LFA 1 ( neg ) 183 + / 62 / 7 , 500 cells 5 LFA 1 ( pos ) 29 + / 17 / 7 , 500 cells , P < . 001 ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We have recently demonstrated that CD11b / dullCD11c+ and CD11b+hiCD11c+ dendritic cell ( DC ) precursor subsets represent two distinct DC differentiation pathways from murine bone marrow lineage phenotype negative ( Lin ) c kit+ hematopoietic progenitor cells ( HPCs ) stimulated with granulocyte macrophage colony stimulating factor ( GM CSF ) + stem cell factor ( SCF ) + tumor necrosis factor alpha ( TNFalpha ) . ^^^ Phenotypically , this inhibitory effect was accompanied by markedly suppressed expression of Ia and CD 86 antigens as well as major histocompatibility complex ( MHC ) class 2 transactivator ( CIITA ) and CC chemokine receptor 7 ( CCR 7 ) mRNAs in Lin c kit+ HPC cultures stimulated with GM CSF + SCF + TNFalpha at day 6 . ^^^ In the absence of TNFalpha , TGF beta 1 markedly suppressed the expression of CIITA and CCR 7 mRNAs in GM CSF + SCF stimulated Lin c kit+ HPCs at either day 6 or day 12 and induced the differentiation solely into monocytes / macrophages as evident in morphology , active phagocytic , and endocytic activities . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Although granulocyte colony stimulating factor ( G CSF ) , interleukin ( IL ) 6 , IL 7 , and IL 11 synergize with SCF to increase blast colony formation , cytokines such as granulocyte macrophage CSF or IL 3 are without significant effect . ^^^ Data indicate a pivotal role for SCF / c kit interaction during antigenic maturation , self renewal , and apoptotic protection of these lineage restricted progenitors during non CSF mediated induction of differentiation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
However , mobilized CD34+ cells were significantly more responsive than their steady state BM counterparts to interleukin 3 ( IL 3 ) and stem cell factor ( SCF ) combined with G CSF or IL 3 in the presence of erythropoietin ( Epo ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Two animals in each group were treated as follows : 200 or 500 microg / kg FL , 10 microg / kg granulocyte colony stimulating factor ( G CSF ) , 10 or 75 microg / kg stem cell factor ( SCF ) , 10 microg / kg G CSF + 500 microg / kg FL , 10 microg / kg G CSF + 75 microg / kg SCF + 500 microg / kg FL . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In contrast , in LFSS patients , low levels of TGF beta and the increased release of GM CSF and SCF maintain the intense erythropoiesis in response to higher erythropoietic stress , in these more severe patients . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Using the panel of specific antibodies the expression of cytokine receptors ( IL 1 , 2 , 3 , 6 , 7 , 8 , 10 , SCF , gp 130 , G CSF , GM CSF , EPO ) and Fas , Pgp and 24 34 kD multidrug resistance associated protein were examined . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We describe the in vitro effect of pegylated recombinant human MGDF ( PEGrHuMGDF ) alone and in combination with other haemopoietic growth factors ( G CSF , GM CSF , IL 3 , IL 6 , erythropoietin , SCF ) on megakaryopoiesis in bone marrow from 11 normal subjects and 19 patients with aplastic anaemia ( AA ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Purified human CD34+ cells were cocultured with PMVECs for 7 days in the presence of optimal concentrations of granulocyte / macrophage colony stimulating factor ( GM CSF ) + interleukin ( IL ) 3 + IL 6 + stem cell factor ( SCF ) + Flt 3 ligand . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In this study we have investigated the effect of the early acting haemopoietic cytokines flt 3 ligand ( FL ) , IL 3 and GM CSF on the SCF dependent differentiation of mast cells from cord blood mononuclear cells . ^^^ The appearance of mast cells was attenuated when the cells had been in culture with IL 3 or GM CSF prior to culture in SCF , compared to cultures exposed to SCF alone for 7 weeks . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Synergistic stimulatory effects of TPO with stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , granulocyte colony stimulating factor and granulocyte macrophage colony stimulating factor were observed in both TPO responding cases ( 9 / 17 ) and TPO non responding cases ( 8 / 13 ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Human cord blood CD 34 ( + ) stem cells were allowed to differentiate in the presence of cytokines stem cell factor ( SCF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) and tumour necrosis factor alpha ( TNF alpha ) into functional CD1a+dendritic cells ( DC ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Different culture conditions were generated by adding G CSF , granulocyte macrophage colony stimulating factor ( GM CSF ) , Flt 3 ligand ( Flt 3 ) , megakaryocyte growth and development factor ( Peg rHuMGDF ) , GM CSF / erythropoietin ( EPO ) hybrid protein ( MEN 11303 ) , and interleukin 15 ( IL 15 ) to the basic stem cell factor ( SCF ) + interleukin 3 ( IL 3 ) + EPO combination . ^^^ Compared to equimolar concentrations of GM CSF and EPO , MEN 11303 hybrid protein showed a significantly higher capacity of expanding CFU GM , BFU E , LTC IC , CD34+ , and CD34+ / CD38 cells when these cytokines were tested in combination with IL 3 / SCF / Flt3 . ^^^ These cultures indicated that Peg rHuMGDF addition to IL 3 / SCF / EPO / Flt3 does not affect CFU GM and BFU E expansion but , unlike G CSF or GM CSF , it does not decrease the ability of Flt 3 to expand primitive LTC IC . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Marked up regulation of growth factors , cytokines and receptor expression was observed in thick ( > 1 mm ) primary melanomas , which were stained with polyclonal or monoclonal antibodies ( MAbs ) for IL 1alpha , IL 1beta , IL 6 , IL 8 , TNF alpha , TGF beta , granulocyte macrophage colony stimulating factor ( GMCSF ) and stem cell factor ( SCF ) , but not IL 2 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Other cytokines stimulating megakaryocytopoiesis in various species using different dosing regimens include Interleukin 1 ( IL 1 ) , Interleukin 3 ( IL 3 ) , Interleukin 6 ( IL 6 ) , Interleukin 11 ( IL 11 ) , stem cell factor ( SCF ) , granulocytemacrophage colony stimulating factor , ( GM CSF ) , fusion proteins , such as promegapoietin , and a peptide agonist of TPO receptor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Culturing cord blood CD34+ cells with granulocyte macrophage colony stimulating factor ( GM CSF ) and tumor necrosis factor ( TNF ) alpha for 12 days , and stem cell factor ( SCF ) for 5 days , resulted in a 40 + / 26 fold expansion in cell numbers , with 38 + / 20 % dendritic cells ( DCs ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In vivo administration of G CSF reduced the adherence capacity of CD34+ cells to normal BM stroma ; in vitro incubation with SCF or IL 3 enhanced the expression of CD49d on CD34+ cells , while GM CSF reduced the expression of CD62L . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
DESIGN AND METHODS : To achieve long term expression of a specific transgene in APCs , G CSF mobilized peripheral blood CD34+ cell populations were retrovirally transduced with the bacterial nlsLacZ , a marker gene used here as a model , in the presence of IL 3 , IL 6 , GM CSF and SCF prior to being induced to differentiate into dendritic and macrophage cells by GM CSF and TNF a . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
G CSF , GM CSF , IL 3 , M CSF , PIXY 321 , SCF ) had synergistic or additive mitogenic effects . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We found that granulocyte macrophage colony stimulating factor ( GM CSF ) optimally activated proliferation of TF 1 cells regardless of the presence or absence of stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Stimulation of human hematopoietic growth factor responsive cell lines with stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , and granulocyte macrophage colony stimulating factor ( GM CSF ) demonstrate the rapid tyrosine phosphorylation of SHIP 2 and its resulting association with SHC . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The cytokines macrophage colony stimulating factor ( M CSF ) and stem cell factor ( SCF ) bind to related members of this tyrosine kinase receptors family , c fms and c kit , respectively . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Although GM CSF had minimal effects on the survival or proliferation of primitive progenitors when added alone , the cytokine enhanced stem cell factor ( SCF ) induced cell cycle entry in the first generation . ^^^ The effect was not observed when cells were incubated sequentially with SCF and GM CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Purified CD34+ cells expanded exponentially and produced 800 to 4000 fold more nucleated cells , 300 to 3500 fold more GM CFU and 10 to 80 fold more HPP CFC in stroma free suspension culture with interleukin 1 ( IL 1beta ) , IL 3 , IL 6 , GM CSF and stem cell factor ( SCF ) , than did the starting cell input . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Endogenous serum thrombopoietin ( TPO ) and various cytokines including erythropoietin ( EPO ) , interleukin ( IL ) 3 , IL 6 , IL 11 , granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) and stem cell factor ( SCF ) levels were measured in five patients with acute promyelocytic leukaemia ( APL ) during all trans retinoic acid ( RA ) treatment . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
First , we investigated whether CD 34 ( + ) stem / progenitor cells express gelatinases A ( MMP 2 ) and / or B ( MMP 9 ) and whether growth factors and cytokines ( granulocyte colony stimulating factor [ G CSF ] , granulocyte macrophage colony stimulating factor [ GM CSF ] , stem cell factor [ SCF ] , macrophage colony stimulating factor [ M CSF ] , interleukin 3 [ IL 3 ] , IL 6 , IL 8 , and tumor necrosis factor alpha [ TNF alpha ] ) are able to modulate their expression . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The recombinant ovine ( rov ) SCF protein has been expressed in Chinese hamster ovary ( CHO ) cells , purified , and its biological activity on ovine bone marrow cells compared with that of interleukin 3 ( rovIL 3 ) , granulocyte macrophage colony stimulating factor ( rovGM CSF ) , interleukin 5 ( rovIL 5 ) , human macrophage colony stimulating factor ( M CSF ) and human erythropoietin ( epo ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Then they were cultured , both in agar and in limiting dilution liquid culture , in the presence of 5637 cell line supernatant ( containing GM CSF , G CSF and interleukin 1 ) , stem cell factor ( SCF ) and interleukin 3 ( IL 3 ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In this trial , stem cell mobilization with granulocyte colony stimulating factor ( G CSF ) alone was compared to mobilization with G CSF combined with stem cell factor ( SCF ) in heavily pretreated patients with Hodgkin ' s and non Hodgkin ' s lymphoma . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We studied the sensitivity of progenitor cells of a CN patient to colony stimulating factors ( CSF ) including G CSF , interleukin 3 ( IL 3 ) , and stem cell factor ( SCF ) . ^^^ While the presence of SCF enhanced the number of G CSF induced colonies regardless of the concentration of G CSF in normal controls , this synergistic effect of SCF was limited to the high concentration of G CSF in the patient , indicating that the abnormality in hematopoiesis in CN involved more immature progenitor cells responsive to SCF . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Cord blood CD34+ cells were then cultured for 14 d with IL 3 , IL 6 , SCF , G CSF and GM CSF , each at 10 ng / ml . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We have tested the efficacy of pig specific cytokines ( PSC ) ( IL 3 , SCF , GM CSF ) in the mobilization of porcine bone marrow ( BM ) progenitors in vivo ( 1 ) in the pig and ( 2 ) in baboons that underwent a conditioning regimen and porcine BM transplantation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The cytokines IL 3 , IL 4 , stem cell factor ( SCF ) , GM CSF , and insulin all induced the rapid and transient activation of PKB in responsive cell lines . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We could demonstrate in HUAEC that stimulation with IL 1alpha and TNF alpha led to the mRNA expression of basic fibroblast growth factor ( bFGF ) and vascular endothelial growth factor ( VEGF ) which are crucial in the process of angioneogenesis and atherosclerosis as well as of the granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) and stem cell factor ( SCF ) which are main growth factors in haematopoiesis . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Using KSOM as the culture medium , the effects of stem cell factor ( SCF ) and granulocyte macrophage colony stimulating factor ( GM CSF ) were studied . ^^^ The combination of SCF with GM CSF had no significant effect on the percentage survival of type A spermatogonial cells . ^^^ These data indicate that SCF and GM CSF play a role in the regulation of survival and / or proliferation of type A spermatogonia . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We examined the effect of recombinant human interleukin ( IL ) 11 alone or in combination with various colony stimulating factors ( CSFs ) , including IL 3 , granulocyte / macrophage ( GM ) CSF , granulocyte ( G ) CSF , stem cell factor ( SCF ) , flt 3 ligand ( FL ) , and thrombopoietin ( TPO ) , on colony formation by leukemic progenitor cells ( L CFU ) obtained from 33 patients with acute myelogenous leukemia ( AML ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
However , addition of human SCF , G CSF , GM CSF , LIF , MIP 1alpha and IL 6 in concentrations found in human marrow stroma conditioned medium to AFT 024 non contact cultures increased LTC IC maintenance to 72 % at 5 weeks . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The present studies showed a significant baseline level of apoptotic cells in short term ( 5 day ) cultures of normal CD 34 ( + ) cells containing GM CSF plus stem cell factor ( SCF ) , and a markedly greater level of apoptosis in cytokine deficient cultures . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In contrast , the presence of both SCF and GM CSF allows for the continuous survival and expansion of hematopoietic progenitor cells in culture , as well as favoring their terminal differentiation along granulocytic and monocytic pathways . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
To clarify this point , the serum level of G CSF was measured using an ELISA , and various other cytokines , including GM CSF , macrophage CSF ( M CSF ) , SCF , IL 6 , IFN gamma , and soluble IL 2 receptor ( IL 2R ) , were tested for comparison in children receiving conventional or high dose chemotherapy and autologous transplantation with unmanipulated or purified PBSC . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In short term cultures containing erythropoietin ( Epo ) , interleukin ( IL ) 1 , IL 3 , and IL 6 , or granulocyte ( G ) and granulocyte macrophage colony stimulating factor ( GM CSF ) together with stem cell factor ( SCF ) or Flt 3 ligand ( FL ) , expansion of CFUs was six to eightfold at week 1 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The Th 2 cytokines IL 3 , IL 5 , IL 6 , IL 9 , and granulocyte / macrophage colony stimulating factor each augmented the SCF dependent proliferation of hPrMCs and hMCs . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We have assessed tumor contamination of peripheral blood progenitor cells ( PBPC ) in 203 high risk breast cancer patients who were prospectively randomized to mobilization with stem cell factor ( SCF ) plus granulocyte colony stimulating factor ( G CSF ) versus G CSF alone . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
However , these enriched cells ( CD56+ , CD 45 , CD 34 ) did not give rise to hemopoietic cells under the culture conditions used , i . e . in the presence of the growth factors IGF 1 , bFGF , SCF , IL 3 , and GM CSF Together , our data do not support the hypothesis that EDC are totipotent mesenchymal progenitors giving rise to hemopoietic cells . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Recombinant human cytokines stem cell factor ( SCF ) , pegylated megakaryocyte growth and differentiation factor ( PEG MGDF ) , FLT 3 L , Interleukin ( IL ) 3 , IL 6 , and granulocyte macrophage colony stimulating factor ( GM CSF ) were systematically added at various concentrations ( 10 to 300 ng / mL ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Monocytic differentiation of CD 34 ( + ) cells supported by FLT 3 ligand and interleukin 3 ( IL 3 ) was correlated to high levels of hypophosphorylated pRb , whereas neutrophilic differentiation , supported by granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) , was correlated to low levels . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The improvement of the procedure of hematopoietic cells transplantation and its efficacy is caused by the stimulation of the hematopoiesis by using the combination of cytokines ( G CSF , GM CSF , IL 3 , SCF ) and purging of the hematopoietic cells obtained from the cellular apheresis . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Finally , purified CD 34 ( + ) CD 40 ( + ) blood cells stimulated an alloreactive T cell response in MLC , were enriched in granulocytic , monocytic , and dendritic precursors , and generated high numbers of DC in 11 14 d liquid cultures with GM CSF , SCF , TNF alpha and FLT 3L . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
After single cell sorting , the CD 34 ( + ) / CD38 ( ) cells derived from FLV were exposed to flt 3 ligand , thrombopoietin , stem cell factor ( SCF ) , or medium containing a cytokine cocktail ( with SCF , interleukin 3 , interleukin 6 , granulocyte macrophage colony stimulating factor , and erythropoietin ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Moreover , the addition of PGG glucan to cultures containing GM CSF + IL 3 or GM CSF + SCF significantly augmented granulocyte macrophage colony production above baseline , demonstrating that PGG glucan acts independently of those early acting cytokines and can enhance their activity in an additive manner . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Preincubation of CD 34 ( + ) cells with stem cell factor ( SCF ) before A 23187 stimulation resulted in primed PLA ( 2 ) activity , whereas interleukin 3 ( IL 3 ) , GM CSF , and tumor necrosis factor alpha had no significant effect . ^^^ When CD 34 ( + ) cells were induced to differentiate , PLA ( 2 ) activity remained responsive to SCF for several days , but after 8 days , at which stage morphological and functional evidence of maturation was occurring , priming of PLA ( 2 ) by SCF could no longer be elicited , whereas responses to GM CSF and IL 3 had developed . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NALM 29 cells responded significantly to the proliferative stimuli of FLT 3 ligand and IL 7 , but not to GM CSF , IL 3 , IL 6 , PIXY 321 or SCF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
First , Baf cells only proliferated in response to BAC OV IL 5 and did not respond to addition of recombinant ovine cytokines granulocyte macrophage colony stimulating factor ( GM CSF ) , IL 1beta , IL 2 , IL 3 , IL 6 , IL 8 , stem cell factor ( SCF ) or IFN gamma at doses from 0 . 01 to 1 microg / well . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In neutralizing experiments on HUVEC derived cytokines , antibodies to both stem cell factor ( SCF ) and granulocyte / macrophage colony stimulating factor ( GM CSF ) were added to the cell cultures . ^^^ Addition of anti SCF antibody or anti GM CSF monoclonal antibody to the culture significantly suppressed HUVEC combined IL 3 and IL 5 induced eosinophil differentiation on day 28 of culture by 49 . 2 and 55 . 0 % , respectively . ^^^ CONCLUSIONS : These results indicate that several cytokines including GM CSF and SCF from HUVEC promote IL 3 and IL 5 induced eosinophil differentiation from CD34+ cells . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Growth factors such as stem cell factor ( SCF ) , granulocyte macrophage colony stimulating factor , and thrombopoietin were reported to upregulate the adhesion of hematopoietic progenitors to immobilized fibronectin through activation of integrin alpha4beta1 and alpha5beta1 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The occurrence of receptors for G CSF , GM CSF , IL 3 and SCF on marrow cells was assessed using immunofluorescent ligand binding assays . ^^^ Incubation of AML cells in serum free medium ( SFM ) + / G + GM CSF , IL 3 , SCF , G + GM CSF + SCF or IL 3 + SCF prior to S phase assessment revealed that priming permutations inclusive of SCF were most effective but also that the baseline level of S phase activity with SFM alone influenced the subsequent response to priming . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We describe here that lineage phenotype negative ( Lin ) ( ) c kit ( + ) hematopoietic progenitor cells ( HPCs ) from day 13 postcoitus ( dpc ) murine fetal liver ( FL ) can generate dendritic cell ( DC ) precursors when cultured in vitro in the presence of PA 6 stromal cells plus granulocyte / macrophage colony stimulating factor ( GM CSF ) + stem cell factor ( SCF ) + Flt 3 ligand ( Flt3L ) for 12 to 14 days , and develop into mature DCs when stimulated with GM CSF plus mouse tumor necrosis factor alpha ( mTNFalpha ) for an additional 3 to 5 days . ^^^ Once FL derived HPCs were cultured without GM CSF , NK1 . 1 ( + ) cells developed in the presence of PA 6 cells + SCF + Flt3L . ^^^ These NK1 . 1 ( + ) cells could develop into DC precursors at an earlier stage of differentiation by reculturing with PA 6 cells + SCF + Flt3L + GM CSF , but they would be irreversibly committed to NK cell precursors without GM CSF after 3 days , suggesting that GM CSF plays a critical role in controlling the transition of DC and NK cell precursors from 13 dpc FL derived Lin ( ) c kit ( + ) HPCs . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We have investigated the serum level of stem cell factor ( SCF ) and granulocyte macrophage colony stimulating factor ( GM CSF ) in relation to a control group and commonly accepted tumour markers , such as carcinoembryonic antigen ( CEA ) and cytokeratin fragment 19 ( CYFRA 21 1 ) . ^^^ Additionally , we have defined the diagnostic sensitivity , specificity , positive predictive value , negative predictive value and receiver operating characteristics ( ROC ) curve of SCF and GM CSF in non small cell lung cancer ( NSCLC ) . ^^^ SCF and GM CSF were determined using enzyme linked immunosorbent assay ( ELISA ) , CYFRA 21 1 was measured by radioimmunoassay and CEA by microparticle enzyme immunoassay . ^^^ There were significant increases in circulating SCF and GM CSF in the lung cancer patients compared to the control group . ^^^ The diagnostic specificity of GM CSF was lower ( 65 % ) than SCF specificity ( 70 % ) , but the GM CSF predictive values were higher in relation to the predictive values of SCF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Infection of MYA 1 cells , a CD4+ T lymphoblastoid cell line , with FeLV A activated expression of macrophage inflammatory protein 1alpha ( MIP 1alpha ) , increased transcript levels of GM CSF ( 8 fold ) , macrophage CSF ( M CSF ) ( 16 fold ) and stem cell factor ( SCF ) ( 250 fold ) , and decreased ( 4 fold ) expression of IL 10 and tumor necrosis factor alpha ( TNF alpha ) . ^^^ Productive infection with four different FIV molecular clones caused progressive MYA 1 cell death ; however , the mRNA expression profiles were unchanged except for 2 to 4 fold increases in M CSF and 16 to 500 fold increases in SCF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Culture of bone marrow precursor cells with cytokines , including granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 4 ( IL 4 ) and the tyrosine kinase receptor binding proteins Flt 3 ligand ( Flt 3L ) and stem cell factor ( SCF ) , has previously been shown , in both mouse and human , to result in the generation of large numbers of dendritic cells . ^^^ Culture of bovine bone marrow cells with GM CSF , IL 4 and either Flt 3L or SCF enhanced the generation of low buoyant density dendritic cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
A large number of cells with the morphological , phenotypical , and functional characteristics of DCs was generated from both c kit ( low ) and c kit ( low ) populations when cultured with a combination of cytokines ( GM CSF , tumor necrosis factor a [ TNF a ] , interleukin 7 [ IL 7 ] , IL 3 , stem cell factor [ SCF ] , and flt 3 ligand ) ; the cytokine combination studies revealed that SCF and IL 3 in addition to GM CSF and TNF a are essential for DCs to be generated from these primitive populations . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Twelve cases of human AML were tested for ability to be differentiated toward a professional APC phenotype in short term culture with addition of GM CSF and the following recombinant proteins : TNFalpha , IL 4 , CD 40 ligand , Flt 3 ligand and SCF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We assessed PEG rHuMGDF combined with granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage CSF ( GM CSF ) , interleukin 3 ( IL 3 ) , IL 6 , stem cell factor ( SCF ) or erythropoietin in 40 MDS , 33 AML and 16 normal bone marrow samples . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Three lines of Tg mice were generated : one carrying porcine IL 3 and GM CSF genes only ( termed IL / GM ) and the remaining two lines carrying in addition , the soluble SCF gene ( termed IL / GM / sS ) or membrane bound SCF gene ( termed IL / GM / mS ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
DCs were derived from CD 34 ( + ) progenitors using SCF plus GM CSF plus TNFalpha . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The addition of Stem Cell Factor ( SCF ) or IL 6 to the standard combination of flt 3L+ / GM CSF produces a large increase in the proliferation of GM and DC progenitors ( 28 times and 11 times respectively ) in the first step of the culture . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Leukemic cells were cultured in the presence or absence of various cytokines including GM CSF , SCF , TNF alpha , IL 4 , and gamma interferon . ^^^ Proliferation ( 5 / 14 ) or increased survival ( 7 / 14 ) of AML cells was observed in the presence of GM CSF , SCF , and TNF alpha . ^^^ GM CSF , SCF , and TNF alpha induced morphologic changes typical for DC and increased the expression of costimulatory and adhesion molecules . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Significant growth responses of leukemic blasts to TPO were seen in 10 / 10 CD7+ and 7 / 20 CD 7 AML cases using 3H thymidine incorporation , while synergistic stimulatory effects of TPO with stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , granulocyte colony stimulating factor , and granulocyte macrophage colony stimulating factor were observed in both groups . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
When cultured with the single cytokines as stem cell factor ( SCF ) , TPO , or interleukin ( IL ) 3 , these cells were maintained or had increased by day 5 , whereas only a few cells survived in cultures with granulocyte colony stimulating factor , IL 11 , or IL 6 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Incubation ( 24 h ) with interleukin 3 ( IL 3 ) , recombinant human stem cell factor ( SCF ) and granulocyte colony stimulating factor ( G CSF ) significantly increased the proportion of cells in the S phase for both CFC and LTC IC without inducing any loss in numbers . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Single human cord blood CD34+CD38 cells were individually cultured by micromanipulation with interleukin ( IL ) 3 , stem cell factor ( SCF ) , fIt 3 ligand ( FL ) , IL 6 and granulocyte colony stimulating factor ( G CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We preinjected the mice with murine stem cell factor ( SCF ) encoding adenovirus ( AdSCF ) and murine granulocyte macrophage colony stimulating factor ( GM CSF ) encoding adenovirus ( AdGM CSF ) ; after 7 days , the mice were inoculated with CT 26 colon adenocarcinoma . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
SCF , IL 1beta , IL 1ra and GM CSF in the bone marrow and serum of normal individuals and of AML and CML patients . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Their production of M CSF cultured in serum free medium is enhanced in a time dependent manner in response to tumour necrosis factor ( TNF ) alpha and interleukin ( IL ) 4 but not to IL 1 , IL 3 , IL 6 , IL 7 , IL 10 , SCF , granulocyte macrophage colony stimulating factor ( GM CSF ) , G CSF , bFGF and transforming growth factor ( TGF ) beta . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Stem Cell Factor ( SCF ) can stimulate the growth and development of primitive multipotential and unipotential hematopoietic stem cells , either alone or in combination with other cytokines such as Granulocyte Macrophage Colony Stimulating factor ( GM CSF ) and Granulocyte CSF ( G CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The adherent stroma cell layer was modified by interleukin 3 ( IL 3 ) + granulocyte macrophage colony stimulating factor ( GM CSF ) + stem cell factor ( SCF ) with an increased cellularity and higher percentage of differentiated myeloid cells and a reduced percentage of stroma cells . ^^^ We have studied the effects of stimulative cytokines , such as GM CSF , SCF , and IL 3 , on proliferation of committed colony forming unit cells ( CFU C ) , LTC IC , and stroma cell formation in LTBMC of unseparated bone marrow cells . ^^^ IL 3 , GM CSF , and SCF significantly stimulated the cumulative proliferation of nucleated cells and committed CFU C . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Recently , cytokines and interleukins such as SCF , GM CSF , G CSF , TGF beta , IL 6 , IL 7 , IL 8 , IL 11 have been reported to be elaborated by endothelial cells . ^^^ Our finding of the existence of mRNAs of SCF , GM CSF , IL 6 , TGF beta , IL 1 , and IL 11 in these cells was in agreement with the data reported previously . ^^^ Among these cytokines and interleukins , SCF , GM CSF , IL 6 , IL 1 , and IL 11 are haematopoietic stimulators which may be responsible for the stimulative effects on the growth of haematopoietic progenitors . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Comparisons of structure function relationships of Flt3L with the homologous hematopoietic cytokines macrophage colony stimulating factor ( MCSF ) and stem cell factor ( SCF ) suggest that they have a common receptor binding mode that is distinct from the paradigm derived from the complex of growth hormone with its receptor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Primitive hematopoietic progenitors were stimulated in liquid cultures and clonogenic assays by early acting growth factors such as stem cell factor ( SCF ) and interleukin 11 ( IL 11 ) and the intermediate late acting stimulating factors IL 3 , granulocyte macrophage colony stimulating factor , and erythropoietin . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
By day 21 , the endothelial based system receiving the FLT3L , SCF , IL 6 , and GM CSF cytokine combination contained five fold greater numbers of CD34+ than the stroma cell free culture cell system . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We examined the effects of granulocyte colony stimulating factor ( G CSF ) , stem cell factor ( SCF ) , and thrombopoietin ( TPO ) , alone or in combination , on the generation of neutrophils by bone marrow ( BM ) cells from three patients with severe congenital neutropenia ( SCN ) through the use of a serum deprived liquid culture system . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In this paper the effects of flt 3 ligand ( FL ) , stem cell factor ( SCF ) , interleukin ( IL ) 3 , IL 6 , granulocyte colony stimulating factor ( G CSF ) and granulocyte monocyte colony stimulating factor ( GM CSF ) on long term ex vivo expansion and differentiation of cord blood hematopoietic cells were investigated . ^^^ In the culture containing FL + SCF + G CSF + GM CSF , the total cell expansion ratio reached the maximum ( 385 . 30 + / 163 . 51 fold ) at 28 days , whereas in the culture with FL + SCF + IL 3 + IL 6 , CFU GMs expansion ratio reached a plateau ( 409 . 52 + / 189 . 50 fold ) at 28 days . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Peripheral blood mononuclear cells ( PBMCs ) from 15 newly diagnosed acute myeloid leukemia ( AML ) patients were cultured in fetal calf serum free media supplemented with either granulocyte / macrophage colony stimulating factor ( GM CSF ) , interleukin ( IL ) 4 and tumor necrosis factor alpha ( TNFalpha ) , or GM CSF , stem cell factor ( SCF ) , TNFalpha and transforming growth factor beta ( TGFbeta ) in order to generate leukemia derived dendritic cells ( DCs ) . ^^^ GM CSF , IL 4 plus TNFalpha was superior in 11 cases , and better results were obtained with GM CSF , SCF , TNFalpha plus TGFbeta in 3 cases . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
A significant reduction of CD 34 positive cells after incubation with a cytokine mix [ interleukin ( IL ) 1beta , IL 3 , IL 6 , stem cell factor ( SCF ) , erythropoietin ( EP ) with granulocyte macrophage / colony stimulating factor ( GM CSF , Cytok 1 ) could be achieved in all 16 cases with a CD 34 positive blast phenotype studied at diagnosis ( P < 0 . 001 ) , in 3 of 10 cases at relapse , and in 8 of 18 cases in complete remission . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CD34+ bone marrow cells of healthy donors were used to generate DCs with the recombinant human cytokines granulocyte macrophage colony stimulating factor ( GM CSF ) , tumour necrosis factor alpha ( TNF alpha ) and stem cell factor ( SCF ) , both with and without interleukin 4 ( IL 4 ) . ^^^ Flow cytometric analysis demonstrated that HLA DM+ cells comprised 2 . 5 + / 0 . 9 % and 1 . 8 + / 0 . 4 % of the CD34+ cell derived progeny in the presence of GM CSF , TNF alpha and SCF after 7 d and 14 d of culture respectively . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The addition of AcSDKP in short term bone marrow cultures resulted in a maximum depression of the total number of progenitor cells as well as the number of progenitor cells entering cell cycle following culture with 10 ( 12 ) to 10 ( 14 ) M AcSDKP and 10 ( 14 ) M AcSDKP when exogenous cytokines ( GM CSF , IL 3 , or SCF ) were added . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Enhanced antitumor effect of suicide gene therapy by SCF , GM CSF gene transfer in vivo and its immunological mechanism ] . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The yield of pre CFUs with the combination of granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) was significantly higher than with G CSF alone , cyclophosphamide ( Cyclo ) and granulocyte monocyte colony stimulating factor ( GM CSF ) , interleukin ( IL ) 3 and GM CSF , or Cyclo alone . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
MC were produced by using GM CSF and SCF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In cultures with SCF , Flt 3 ligand ( FL ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and tumor necrosis factor ( TNF ) alpha ( standard condition ) , CD 7 ( + ) CD45RA ( + ) progenitors expanded less ( 37 vs 155 fold ) but yielded 2 fold higher CD1a ( + ) DC percentages than CD 7 ( ) CD45RA ( + ) or CD 7 ( + ) CD45RA ( ) progenitors . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Nonenriched CB cells were cultured for 21 days in the presence of conditioned medium from the HS 5 stromal cell line and FLT 3 L or alternatively in the presence of FLT 3 L , stem cell factor ( SCF ) , megakaryocite growth and development factor ( MGDF ) , and granulocyte colony stimulating factor ( G CSF ) ( FSMG ) , either on fibronectin fragment CH 296 coated dishes or on uncoated dishes . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In this article we describe the differentiation and expansion of lentivirus vector marked DCs from umbilical cord blood , bone marrow , and cytokine mobilized peripheral blood CD 34 ( + ) cells in the presence of GM CSF , TNF alpha , SCF , Flt 3 , and IL 4 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
PMVEC coculture supplemented with GM CSF + IL 3 + IL 6 + SCF + Flt 3 ligand induced > 93 % of the CD 34 ( + ) CD 38 ( ) population to enter the G ( 1 ) or G ( 2 ) / S / M phase while increasing this population from 1 . 4 % on day 0 to 6 . 5 % of the total population by day 5 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Megakaryocytopoiesis is a complex network regulated by different megakaryocyte ( MK ) stimulating factors ( i . e . , thrombopoietin [ TPO ] , stem cell factor [ SCF ] , interleukin 3 [ IL 3 ] , IL 6 , IL 11 and GM CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
When stimulated in agar cultures by GM CSF , G CSF , M CSF , IL 3 , SCF , IL 6 , leukemia inhibitory factor ( LIF ) , IL 5 or IFNgamma , GB 2 cells generated blast colonies or colonies of maturing granulocytes and macrophages . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Following high dose chemotherapy administration , we transplanted adult patients with CB expanded in static culture for 10 days , in DM containing stem cell factor ( SCF ) , granulocyte colony stimulating factor ( G CSF ) , and megakaryocyte growth and development factor ( MGDF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In physical or biological characteristics , it exhibited a unique character different from IL 3 , IL 6 , GM CSF , SCF or FLT 3 ligand those are known to have hematopoietic stem cell proliferation stimulating activity . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In serum free liquid cultures supplemented with stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) and granulocyte colony stimulating factor , the expression of surface CXCR 4 progressively increased in cells differentiating along the granulocytic lineage . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Hemopoietic cells were grown in long term cultures in the presence of various combinations of early acting cytokines such as FLT 3 ligand ( FLT 3 L ) and stem cell factor ( SCF ) and the differentiating growth factors GM CSF and TNF alpha . ^^^ Conversely , FLT 3 L and SCF do not directly affect DC differentiation , but instead sustain the long term expansion of CFU DC , which can be induced to produce mature DC by GM CSF and TNF alpha . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
MSCs constitutively expressed mRNA for interleukin ( IL ) 6 , IL 11 , leukemia inhibitory factor ( LIF ) , macrophage colony stimulating factor ( M CSF ) , and stem cell factor ( SCF ) . ^^^ MSCs treated with IL 1alpha upregulated mRNA levels of IL 6 , IL 11 , and LIF , and began to express detectable levels of granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) . mRNA levels of M CSF and SCF did not change . ^^^ MSCs cultured in osteogenic medium differentiated along the osteogenic lineage and downregulated mRNA levels of IL 6 , IL 11 and LIF whereas , M CSF and SCF expression were unchanged and G CSF and GM CSF remained undetectable . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
To study this , we incubated peripheral blood lymphocytes from CLL patients with the following cytokines : EPO , SCF , TNFalpha , TGFbeta , GMCSF , TPO , IL 1 , IL 2 , IL 3 , IL 4 , GCSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Therefore BM cells were ( a ) either frozen in liquid nitrogen or ( b ) precultured for 14 days with cytokines : IL 1beta , IL 3 , IL 6 , erythropoietin ( EPO ) , stem cell factor ( SCF ) with ( ' Cytok1 ' ) or without granulocyte macrophage colony stimulating factor ( GM CSF ) ( ' Cytok2 ' ) or with no added cytokines ( ' ISC / FCS ' ) as a control . ( 3 ) Southern blot analysis was used to detect clonal BM cells . ^^^ However the cytotoxic effect of LAK cells was more pronounced if cytokines ( especially GM CSF and SCF ) were comprised . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Soluble dlk IgG Fc chimeric protein completely inhibited the colony formation of lineage marker negative ( Lin ) bone marrow cells by GM CSF , G CSF , or macrophage CSF ( M CSF ) in the presence of stem cell factor ( SCF ) . ^^^ Furthermore , dlk failed to inhibit the colony formation of Hes 1 null fetal liver cells by M CSF in the presence of SCF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
RESULTS : Different combinations of HGFs , including SCF , IL 3 , IL 6 , GM CSF , Epo and Tpo increased BFU E , CFU GM , CFU MK and CD41a+ cells by 14 . 97 + / 2 . 89 fold , 14 . 46 + / 3 . 19 fold , 34 . 67 + / 4 . 62 fold and 17 . 29 + / 2 . 34 fold , respectively . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In a second set of experiments , CB derived progenitor cells were incubated with interleukin ( IL ) 3 , stem cell factor ( SCF ) and granulocyte colony stimulating factor ( G CSF ) and their cell cycle status assessed both by the cytosine arabinoside ( Ara C ) suicide approach and by flow cytometric DNA analysis . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The effect of granulocyte colony stimulating factor ( G CSF ) induced mobilization of peripheral blood progenitor cells ( PBPC ) on the endogenous serum levels of cytokines stem cell factor ( SCF ) and flt 3 ligand ( flt 3 L ) was studied in 18 healthy subjects undergoing allogeneic PBPC donation . ^^^ Endogenous serum flt 3 L and SCF were determined prior to G CSF administration , on the day of leukapheresis , and followed up until day +100 after cessation of G CSF administration . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We have already shown that cytokine cocktails ( IL 1beta , IL 3 , IL 6 , SCF , GM CSF ) and / or lymphokine activated killer ( LAK ) cells can reduce the amounts of clonal , CD 34 positive mononuclear bone marrow cells ( BM MNC ) in acute myeloid leukemia ( AML ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In the present study , we examined the effect of IL 3 on human B lymphopoiesis using the co culture system on a murine stromal cell line , MS 5 , with stem cell factor ( SCF ) and granulocyte colony stimulating factor ( G CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
However , in vitro expansion and differentiation in the presence of GM CSF , TNF alpha , Flt 3L , SCF and IL 4 resulted in a reduction in the percentage of DC expressing the transgene . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Blast cells were expanded in serum free medium supplemented with Flt3L , G CSF , GM CSF , IL 3 , IL 6 and SCF for 7 days and subsequently stimulated with Flt3L , GM CSF and TGF beta for a further 14 days , with the addition of TNF alpha for the final 48 h of culture . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
DCs could be induced either from short or long term cultured CD34+ cells after switching the cytokines from Flk 2 / Flt 3 ligand , stem cell factor ( SCF ) , thrombopoietin ( TPO ) to granulocyte macrophage colony stimulating factor ( GM CSF ) and interleukin 4 ( IL 4 ) ( immature type ) plus tumor necrosis factor ( TNF ) alpha with stimulation by CD40L transfectant ( mature type ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The cells were cultured with four cytokines : interleukin 3 ( IL 3 ) , thrombopoietin ( TPO ) , stem cell factor ( SCF ) , and Flt 3 ) ; five cytokines , IL 3 , TPO , SCF , Flt 3 plus granulocyte macrophage colony stimulating factor ( GM CSF ) , or erythropoietin ( Epo ) ; or all six cytokines in combination . ^^^ After 16 days , significant expansion of MK precursors ( CD 41 ( + ) ) and stem cells ( CD 34 ( + ) and AC 133 ( + ) cells ) were seen in cells cultured in IL 3 , TPO , SCF , and Flt 3 with or without GM CSF compared to the combinations that contained Epo ( p < 0 . 05 ) . ^^^ Similar studies were performed using liquid culture medium , and after 14 days the number of MNCs , CD 34 ( + ) , AC 133 ( + ) , CD 41 ( + ) , and CD 61 ( + ) cells were higher in the UCB cells cultured in IL 3 , TPO , SCF , and Flt 3 compared to those cultured with those four cytokines plus GM CSF . ^^^ These results demonstrate that UCB stem cells can be effectively expanded ex vivo and enriched with platelet precursors using TPO , SCF , Flt 3 , and IL 3 , whereas the addition of Epo and GM CSF is unnecessary . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CD 34 ( + ) cells from the bone marrow of 22 RA patients differentiated into cells with fibroblast like morphology , which expressed prolyl 4 hydroxylase , in the presence of stem cell factor ( SCF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and tumor necrosis factor alpha ( TNF alpha ) , much more effectively than CD 34 ( + ) cells from bone marrow of 15 control subjects ( 10 patients with osteoarthritis and 5 healthy individuals ) . ^^^ The generation of fibroblast like cells was not at all observed in cultures with SCF , GM CSF , and interleukin 4 ( IL 4 ) with or without TNF alpha . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
By screening a panel of secreted factors , granulocyte macrophage colony stimulating factor ( GM CSF ) , interferon ( IFN ) gamma , and stem cell factor ( SCF ) were found induced by HTLV 2 in TF 1 cells . ^^^ Neutralization experiments indicated that GM CSF and IFN gamma , but not SCF , were responsible for HTLV 2 induced STAT activation , whereas anti GM CSF antibodies greatly inhibited TF 1 cell proliferation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Langerhans cells can originate in vitro from immature precursors stimulated with granulocyte macrophage colony stimulating factor ( GM CSF ) , tumour necrosis factor ( TNF ) alpha and stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
METHODS : Murine bone marrow derived Lin c kit+ HPCs were isolated by a cell sorter and cultured in the presence of granulocyte macrophage colony stimulating factor ( GM CSF ) , stem cell factor ( SCF ) , tumor necrosis factor alpha ( TNF alpha ) and TGF beta 1 . ^^^ RESULTS : TGF beta 1 could significantly inhibit the generation of DC precursors at day 6 and mature DCs at day 12 from murine Lin c kit+ HPCs stimulated with GM CSF + SCF + TNF alpha or GM CSF + SCF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The rhTpo induced proliferation of AML cells was enhanced when combined with IL 3 , GM CSF or SCF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
To this end , we compared expression of RNA encoding the receptors for interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , IL 6 , leukemia inhibitory factor ( LIF ) and stem cell factor ( SCF ) in populations of primitive hematopoietic progenitors ( lineage marker negative , Lin ( ) , and Lin ( ) c Kit ( + ) ) by RT PCR and in situ RT PCR . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Meis1a suppresses differentiation by G CSF and promotes proliferation by SCF : potential mechanisms of cooperativity with Hoxa 9 in myeloid leukemia . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In the first phase of the study ( Protocol Refinement ) , two SOPs were assessed , by using two cell culture media ( Test A , containing GM CSF ; and Test B , containing G CSF , GM CSF , IL 3 , IL 6 and SCF ) , and the two tests were applied to cells from both human ( bone marrow and umbilical cord blood ) and mouse ( bone marrow ) CFU GM . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
RESULTS : Cultures of cord blood CD34+ cells with the combination of GM CSF + TNF alpha + SCF + FL yielded ( 27 . 18 + / 1 . 56 ) % CD1a+ cells , much higher than that ( 0 . 65 + / 0 . 38 ) % with GM CSF alone . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
When cultured in a semi liquid methylcellulose medium , there were more eosinophil colony forming units grown from asthmatic non adherent mononuclear cell depleted of T cells in the presence of the growth factors GM CSF , SCF and IL 3 , but not of IL 5 . 3 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The combination of thermal injury plus PAO 1 infection enhanced the expression of several pro inflammatory and haematopoietic cytokines [ stem cell factor ( SCF ) , leukocyte inhibitory factor ( LIF ) , IL 6 and TNF alpha ] ; induced the expression of granulocyte macrophage colony stimulating factor ( GM CSF ) and G CSF by 5 h and the expression of additional cytokines , including TGF beta , TNF beta , lymphotoxin beta ( LT beta ) , interferon gamma ( IFN gamma ) , and IFN beta by 40 h post burn / infection . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Stem Cell Factor ( SCF ) + IL 6 and FLT 3 ligand , which have no effect on colony formation by themselves , were used to enhance the effects of IL 3 and GM CSF , respectively . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Optimal growth and DC characteristics were obtained with GM CSF , FL , and SCF in 3 5 day cultures , suggesting a practical strategy for the immunotherapy of leukemia . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Colony formation from normal bone marrow was not increased if stromal layers were incubated with 100 ng / mL granulocyte colony stimulating factor ( G CSF ) or stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The two step method consists of 10 days of first step culture for the expansion and proliferation of CD34+ hematopoietic progenitor cells in the presence of SCF , IL 3 , IL 6 , G CSF , and 7 11 days of second step culture for the induction of DC in the presence of GM CSF , IL 4 and TNF alpha . ^^^ With this two step culture , the absolute number of CD1a+ cells that co expressed HLA DR , CD 80 , CD 40 and CD 58 was enhanced approximately 3 times in CB cell culture and 1 . 9 times in BM cell culture , compared with the commonly used one step culture method for the generation of DC from CD34+ cells using SCF , GM CSF and TNF alpha . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
METHODS : Hematopoietic cells from human umbilical cord blood were cultured with G CSF , GM CSF , rIL 3 , rIL 6 , SCF and Epo in a long term culture system , the cell cycle and cellular markers were analysed dynamically . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
DCs were induced from cord blood CD ( 34 ) ( + ) cells with a combination of cytokines ( GM CSF , TNF alpha , SCF and FL ) and ( 3 ) H TdR was adopted to determine the proliferation of T cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Synergistic effects with the haematopoietic cytokines stem cell factor ( SCF ) and granulocyte macrophage colony stimulating factor ( GM CSF ) were observed . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Equivalent yields of MP were obtained using TPO and one of IL 1 , IL 3 , granulocyte macrophage colony stimulating factor or SCF , suggesting that large cytokine combinations are not necessary for this procedure . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Tissue sections were tested using immunohistochemistry for the expression of keratinocyte derived cytokines with stimulating activity , such as granulocyte monocyte colony stimulating factor ( GM CSF ) , basic fibroblastic growth factor ( bFGF ) , and stem cell factor ( SCF ) or with inhibiting activity , such as interleukin 6 ( IL 6 ) and tumour necrosis factor alpha ( TNF alpha ) on melanocytes . ^^^ In vitiligo skin a significantly lower expression of GM CSF , bFGF and SCF was found , and a significantly higher expression of IL 6 and TNF alpha was detected , compared with perilesional , non lesional and healthy skin . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
When estimating the general profile of the cytokines of these thymocytes by PCR with revertase , it was demonstrated the cells in question did not express cytokines with colony stimulating activities ( SCF , IL 3 , or GM CSF ) or cytokines affecting the migration of bone marrow stem elements ( IL 2 , 4 , or 7 ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Lin ( ) Sca 1 ( + ) HPC cultured 9 days in 100 ng / mL stem cell factor ( SCF ) , 20 ng / mL interleukin 3 ( IL 3 ) , 50 ng / mL monocyte colony stimulating factor ( M CSF ) , 5 ng / mL granulocyte monocyte colony stimulating factor ( GM CSF ) , and 25 ng / mL FLT 3 ligand ( FLT 3 L ) proliferate 387 fold and differentiate into DC precursors . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Best results were achieved when 24 h of prestimulation were followed by a 24 48 h transduction period in the presence of the CH 296 FN fragment and IL 3 , IL 11 , SCF , erythropoietin ( EPO ) , and GM CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Cytokine stimulated stem cell factor ( SCF ) and granulocyte colony stimulating factor ( G CSF ) purified sca ( + ) / lin ( ) cells of C57BL / 6 ( H 2b , 1E ( + ) ) background were injected into MHC mismatched BALB / c ( H 2d , 1E ( ) ) fetal mouse recipients at day 13 of gestation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Recipient mice were treated for 1 or 7 days with growth factors ( stem cell factor [ SCF ] plus interleukin 11 [ IL 11 ] , SCF plus Flt 3 ligand [ FL ] , or granulocyte colony stimulating factor [ G CSF ] ) prior to low dose TBI ( 4 Gy ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Using bone marrow cells as the source of precursors we generated `` myeloid type ' ' DCs from the adherent population using granulocyte macrophage colony stimulating factor ( GM CSF ) , IL 4 and Flt 3L or `` lymphoid type ' ' DCs from the non adherent population using cytokines which included IL 7 , IL 3 , SCF and TNFalpha . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Here we report that Nup 98 HoxA9 , indeed mimicks Hoxa 9 plus Meis 1 coexpression it immortalizes myeloid progenitors , prevents differentiation in response to GM CSF , IL 3 , G CSF , and permits proliferation in SCF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Using in vitro progenitor assays , serum free in vitro cultures , and the nonobese diabetic / severe combined immune deficient ( NOD / SCID ) ecotropic murine virus knockout xenotransplantation model to detect human SCID repopulating cells ( SRCs ) with multilineage reconstituting function , we have characterized and compared purified subpopulations harvested from the peripheral blood ( PB ) of patients receiving granulocyte colony stimulating factor ( G CSF ) alone or in combination with stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
METHOD : The CD ( 34 ) ( + ) CD ( 59 ) ( + ) and CD ( 34 ) ( + ) CD ( 59 ) ( ) cells from PNH patients and CD ( 34 ) ( + ) CD ( 59 ) ( + ) cells from normal volunteers were sorted as each single cell into a well of 96 well culture plates containing culture medium supplemented with SCF , IL 3 , Epo , GM CSF , G CSF , IL 6 , Tpo and Flt 3 ligand . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The synergy between stem cell factor ( SCF ) and granulocyte colony stimulating factor ( G CSF ) : molecular basis and clinical relevance . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The cells were cultured for 12 days in the presence of GM CSF , SCF , FL , IL 4 and TNF alpha to get mature DC enriched population . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Importantly , the maturational effects of TRAIL were observed also in primary normal CD 34 ( + ) cells , seeded in serum free liquid cultures for 4 to 8 days in the presence of SCF + GM CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The observed activity of SCF in marrow cultures was relatively weak , but its action may be stronger in vivo , and SCF needs to be added to GM CSF , IL 5 , and IL 3 in the list of cytokines able to stimulate eosinophil formation . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In serum free suspension cultures of CD34+ cells , elastase completely abrogated the proliferation induced by G CSF but not that of GM CSF or stem cell factor ( SCF ) . ^^^ When exposed to enzymatically active elastase , G CSF , but not GM CSF or SCF , was rapidly cleaved and rendered inactive . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Macrophage colony stimulating factor , granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) and interleukin 7 ( IL 7 ) are potent cytokines belonging to the same structual class that may affect function , growth and apoptosis both in the heart and other organs . ^^^ The aims of the present study were to characterize a post infarction model in the mouse and to examine mRNA expression of M CSF , GM CSF , SCF , IL 3 and IL 7 during the development of heart failure . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Using a nuclease protection assay we investigated mRNA expression of stem cell factor ( SCF ) , a crucial survival factor for mast cells , and the macrophage growth factors macrophage colony stimulating factor ( M CSF ) and granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Moreover , OGP 10 14 preserves stem cells self renewal potential in long term culture ( LTC ) initiating cells and acts directly on CD34+ enriched cells or by increasing activity of stem cell factor ( SCF ) and granulocyte megakaryocyte colony stimulating factor . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
STUDY DESIGN AND METHODS : The effects of three different mobilization regimens stem cell factor ( SCF ) plus granulocyte colony stimulating factor ( G CSF ) plus cyclophosphamide ( CCP ) , G CSF alone , and G CSF plus CCP on the composition of LPs from patients with nonhematologic PBPC malignancies compared to LPs from G CSF mobilized healthy donors and normal marrow ( BM ) samples were analyzed . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Cloning of canine GM CSF and SCF genes . ^^^ In order to determine the complementary DNA ( cDNA ) of canine GM CSF and canine SCF , cDNA clones were generated from lipopolysaccharide ( LPS ) stimulated peripheral blood mononuclear cells ( PBMCs ) and bone marrow cells by reverse transcription PCR amplification . ^^^ The canine GM CSF cDNA obtained in this study contains an open reading frame encoding 144 amino acid residues and has 53 75 % homology with those of human , cat , sheep , pig , cow and mouse , Canine SCF cDNA consist of an open reading frame encoding 274 amino acid residues and shares 81 92 % homology with those of human , cat , pig , cow and mouse . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
TTA inhibited AML blast proliferation in the presence of single cytokines ( GM CSF and SCF : P > 0 . 05 , 35 patients with detectable proliferation ) and a combination of cytokines ( P < 0 . 005 , n = 21 ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Bone marrow was aspirated from the ilium and / or tuber ischiae after administration of recombinant human stem cell factor ( SCF ) and granulocyte colony stimulating factor ( G CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Synergism between stem cell factor ( SCF ) and granulocyte macrophage colony stimulating factor ( GM CSF ) has been shown to be essential for hematopoietic cell proliferation . ^^^ Since HML 2 cells proliferate exponentially in the presence of SCF and GM CSF together , we analyzed the molecular mechanism of the interaction between these two factors in the cells . ^^^ An immediate early gene product , c myc , was additively upregulated in HML 2 cells by addition of a combination of SCF and GM CSF . c myc antisense oligonucleotides effectively suppressed cell proliferation and downregulated the induction of D 3 , E , A , and B cyclins in HML 2 cells stimulated with the two factor combination . ^^^ Taken together , it appears that the induction of c myc is an indispensable event in the proliferation of HML 2 cells and that the cytokines SCF and GM CSF interact reciprocally for expression of all cyclins required for cell cycle progression . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In most nonhuman primate studies , hematopoietic stem cells ( HSCs ) collected from the peripheral blood or bone marrow after administration of granulocyte colony stimulating factor ( G CSF ) + stem cell factor ( SCF ) have been used as targets , but this cytokine combination is not generally available for clinical use , and the optimum target cell population has not been systematically studied . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CD34+ cells from cord blood ( CB ) , bone marrow ( BM ) , or peripheral blood ( PB ) were expanded in a medium containing stem cell factor ( SCF ) , flt 3 ligand ( Flt3L ) and thrombopoietin ( TPO ) with or without HESS 5 , a murine BM stromal cell line , for 2 weeks ( the first expansion step ) , then differentiated to DC in a medium containing granulocyte macrophage colony stimulating factor ( GM CSF ) , flt 3 ligand ( Flt3L ) , stem cell factor ( SCF ) , tumor necrosis factor alpha ( TNF alpha ) , IL 4 , and lipopolysaccharide ( LPS ) for 9 days ( the second differentiation step ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Based on granulocyte colony stimulating factor ( G CSF ) and erythropoietin ( EPO ) alone serve as a baseline with which to compare the effects of the combinations with stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) or granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^ The effects of erythropoietin and recombinant cytokines ( G CSF , SCF , IL 3 and GM CSF ) on colony formation and self renewal by erythroid burst forming units ( BFU E ) and granulocyte macrophage progenitors ( CFU GM ) from mobilized peripheral blood progenitor cells ( PBPCs ) of the normal donors and patients were investigated . ^^^ There was a significantly increased frequency of CFU GM in the presence of SCF , IL 3 , and GM CSF used in conjunction with G CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The component above 10 kD combined with IL 3 , IL 6 , EPO , SCF or GM CSF further enhanced HPP CFC colony formation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
EPO acts synergistically with several growth factors ( SCF , GM CSF , 1L 3 , and IGF 1 ) to cause maturation and proliferation of erythroid progenitor cells ( primarily colony forming unit E ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The ability of acute myeloid leukaemia ( AML ) cells to acquire dendritic cell ( DC ) like characteristics in vitro with a rapid culture method based either on the phorbol ester PMA or calcium ionophores has been studied in comparison to conventional AML DC cultures with the cytokines granulocyte macrophage colony stimulating factor ( GM CSF ) , tumour necrosis factor alpha ( TNF alpha ) , interleukin 3 ( IL 3 ) , SCF , FLT 3 L and IL 4 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
These findings were positively correlated with the up regulation of cytokine mRNA expression such as granulocyte colony stimulating factor ( GM CSF ) , erythropoietin ( EPO ) , thrombopoietin ( TPO ) , stem cell factor ( SCF ) , and c Kit . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
METHODS : Purified 1 10 10 ( 4 ) circulating CD34+ cells isolated from rHuEPO hyporesponsive HD patients ( EPO H ; n = 10 ) , rHuEPO responsive non HD patients with CRF ( EPO R ; n = 8 ) , nonanemic HD patients without rHuEPO therapy ( EPO W / O ; n = 10 ) , and healthy volunteer controls ( CON ; n = 10 ) were subjected to a methylcellulose culture system supplemented with rHuEPO , recombinant human interleukin 3 ( IL 3 ) , recombinant human stem cell factor ( SCF ) , and recombinant human granulocyte macrophage colony stimulating factor ( GM CSF ) for 14 days . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CD 34 progenitor cells were first amplified in vitro with stem cell factor ( SCF ) , Flt 3 ligand ( FL ) , thrombopoietin and IL 6 / soluble IL 6 receptor fusion protein , and cells were then induced to differentiate into DC with IL 4 and GM CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
RT PCR analysis showed that HYMEQ 5 produced FL , SCF , interleukin 6 , and macrophage colony stimulating factor ( M CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CD 34 ( + ) cells purified from ten CBs by immunomagnetic selection were cultured in serum free essential media in the presence of a combination of the several cytokines including EPO , thrombopoietin , flt 3 ligand ( FL ) , stem cell factor ( SCF ) , granulocyte colony stimulating factor , interleukin ( IL ) 3 and / or IL 11 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
When vibrissal follicles of adult mice were cultured in the presence of stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , IL 7 , granulocyte macrophage colony stimulating factor , and Flt 3 ligand , CD45+ / lineage / c kit+ / FcepsilonRI+ cells became detectable on the outgrowing fibroblasts in 10 days and expanded progressively thereafter . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
To study the biological role of human cultured bone marrow mesenchymal stem cell ( BM MSC ) in hematopoiesis by investigation of its expression of multiple hematopoietic growth factors , RT PCR was used to analyze the expression of SCF , Flt 3 ligand , TPO , LIF , G CSF , GM CSF , IL 3 , IL 6 and IL 11 at mRNA level for human BM MSC from healthy donors and patients with leukemia and lymphoma . ^^^ The results clearly showed that the cultured BM MSC expressed mRNA of SCF , Flt 3 ligand , TPO , LIF , IL 6 and IL 11 at passages 3 up to 15 , but did not express G CSF , GM CSF and IL 3 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
DESIGN AND METHODS : Cord blood mononuclear cells were co cultured in a liquid culture system containing the hematopoietic cytokines interleukin ( IL ) 1 , IL 3 , IL 6 , stem cell factor ( SCF ) , granulocyte monocyte colony stimulating factor ( GM CSF ) and G CSF for a total of 20 days with or without depletion of NK cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Several hematopoietic growth factors including interleukin 3 ( IL 3 ) , IL 6 , granulocyte macrophage colony stimulating factors ( GM CSF ) , granulocyte colony stimulating factor ( G CSF ) , and stem cell factor ( SCF ) have been reported to be positive regulators of granulopoiesis and act at different stages of myeloid cell development . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
METHODS : Tissue sections from patients with different types of urticaria and healthy controls were studied for the immunohistochemical expression of known mast cell growth factors ( stem cell factor , SCF ; nerve growth factor , NGF ) , of the inhibitory granulocyte macrophage colony stimulating factor ( GM CSF ) , and of the corresponding receptors , using the alkaline phosphatase antialkaline phosphatase technique . ^^^ RESULTS : Compared to skin of normal controls , staining for SCF , but not for NGF and GM CSF , was significantly decreased in epidermis , endothelium and perivascular cells in lesional and nonlesional skin of all urticarias . ^^^ In contrast , expression of the NGF receptor tyrosine kinase and of the SCF and GM CSF receptors was unchanged . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In 10 day suspension cultures of normal or CML CD34+ cells supplemented with G CSF , stem cell factor ( SCF ) , and granulocyte macrophage colony stimulating factor ( GM CSF ) , CML cells had diminished sensitivity to the growth inhibitory effect of elastase . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Recombinant porcine ( rp ) SCF and granulocyte macrophage colony stimulating factor ( GM CSF ) were expressed in the mammalian HEK 293 cell based expression system . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We examined the effects of thrombopoietin ( TPO ) in combination with stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , and granulocyte colony stimulating factor ( G CSF ) on the proliferation and differentiation of human neutrophils . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
SCF enhances IgE dependent release of mediators from mast cells , including histamine , leukotrienes , cytokines ( TNF alpha , IL 5 , GM CSF ) and chemokines ( RANTES / CCL5 , MCP 1 / CCL2 , TARC / CCL17 e MDC / CCL22 ) ; it is required for IL 4 production in mast cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In cultures with only lineage unspecific cytokines ( IL 3 , SCF , GM CSF ) , BAALC transcripts persisted up to day 20 , while CD 34 transcripts disappeared earlier . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Therefore , in the present study , we have investigated in colorectal cancer patients the serum levels of selected HGFs , such as stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and M CSF in relation to controls and to the classical tumor markers , such as carcinoembryonic antigen ( CEA ) and carbohydrate antigen 19 9 ( CA 19 9 ) in colorectal cancer . ^^^ There were significant differences in the levels of circulating SCF , IL 3 , M CSF , GM CSF , and CEA and CA 19 9 in the colorectal cancer patients compared to the control group . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Enhanced antileukemic activity of allogeneic peripheral blood progenitor cell transplants following donor treatment with the combination of granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) in a murine transplantation model . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The multiple number of CFU GM increased from 2 . 3 + / 0 . 8 to 7 . 8 + / 1 . 9 times when Ang 2 was added into SCF + G CSF + GM CSF + IL 3 combination . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
GM CSF dependent human cell line MO7e was stimulated with GM CSF with SCF , and investigated activation of p70S6K by using phospho specific antibody . ^^^ A possible contribution of p70S6K to SCF synergy was examined by measuring p 21 induction as a model system . p70S6K was slightly activated by GM CSF alone and markedly activated by SCF alone . ^^^ These data suggest that synergistically activated p70S6K by GM CSF plus SCF involves , at least in part , protein translational control including regulation of p 21 protein . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
RESULTS : Proliferation by CFU GM and BFU E was enhanced synergistically when common beta chain receptor cytokines ( IL 3 or GM CSF ) were combined with G CSF or EPO , but not with gp 130 receptor cytokines ( LIF or IL 6 ) or tyrosine kinase receptor cytokines ( SCF , HGF , Flt 3 ligand , or PDGF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
HGF including SCF , FL , G CSF , GM CSF , M CSF and IL 6 were expressed in hPDAC . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Moreover , cell lines expressed interleukin ( IL ) 1 beta , IL 6 , granulocyte macrophage colony stimulating factor ( GM CSF ) , G CSF and stem cell factor ( SCF ) , and secreted variable amount of IL 1 beta , IL 6 and GM CSF . ^^^ However , the inductive capacity was significantly higher in the case of cryopreserved cells , where the rise in clonal growth reached that induced by the addition to the culture media of IL 3 , GM CSF and SCF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Therefore , we examined the expression of receptors for stem cell factor ( SCF ) , interleukin 6 ( IL 6 ) , IL 3 , granulocyte colony stimulating factor ( G CSF ) and IL 7 on the CD 34 ( + ) cells of cord blood , mobilized peripheral blood and bone marrow . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : A human osteoclastogenesis model , using as precursors colony forming unit granulocyte macrophage ( CFU GM ) colonies generated from umbilical cord mononuclear cells cultured in methylcellulose with GM CSF , interleukin ( IL ) 3 and stem cell factor ( SCF ) , has been developed . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The FLCM was assayed for the presence of four principal colony stimulating cytokines , namely stem cell factor ( SCF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) , and erythropoietin ( Epo ) . ^^^ While GM CSF , IL 3 , and Epo were present in insignificant amounts or were altogether absent , 50 635 pg / ml of SCF was found in 8 of the 13 FLCM samples tested . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
This study demonstrates that PU . 1 ( / ) fetal erythroid progenitors are synergistically expanded by TPO plus SCF , but not combinations of EPO plus SCF , IL 3 or GM CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Results of preclinical and clinical growth factor therapy studies with G CSF , GM CSF , pegylated G CSF , SCF , and IL 3 are summarized . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
This study investigated whether mobilization of polymorphonuclear cells ( PMN ) by granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) enhances immigration of opioid containing PMN and peripheral opioid analgesia in rats with Freund complete adjuvant induced hind paw inflammation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Dendritic cells were generated from cord blood CD34+ cells by culture with GM CSF , SCF , and TNF alpha , or from peripheral blood adherent cells or CD14+ cells by culture with GM CSF and IL 4 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
BUC ID suppressed the production of solubleVCAM 1 by RA bone marrow CD34+ cells stimulated with SCF , GM CSF and TNF alpha more effectively than BUC . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Although inhibition of c Kit kinase activity completely blocked SCF induced proliferation , there was still greater than additive growth induced by SCF in combination with GM CSF . ^^^ In contrast , an inhibitory antibody against the extracellular domain of c Kit ( K 27 ) completely inhibited growth in response to SCF alone or in combination with GM CSF . ^^^ These results support a kinase independent component of the synergistic growth induced by SCF and GM CSF that may relate to interaction of these receptors . ^^^ Although synergistic increases in phosphorylation of c Kit and the beta chain of the GM CSF receptor were not observed , SCF and GM CSF in combination prolonged the duration of Erk1 / 2 phosphorylation in a phosphatidylinositol 3 kinase dependent manner . ^^^ Consistent with these findings , phosphatidylinositol 3 kinase is synergistically activated by SCF and GM CSF together . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Several cytokines , especially stem cell factor ( SCF ) and FLT 3 ligand ( FL ) , have been identified as essential to produce large numbers of myeloid precursors and even to increase DC yield obtained by the action of granulocyte macrophage colony stimulating factor ( GM CSF ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We report the results of experiments with CD 34 ( + ) cells from cord blood ( CB , n = 13 ) , bone marrow ( BM , n = 4 ) , and mobilized peripheral blood stem cells ( PBSC , n = 5 ) using two combinations of cytokines : ( A ) granulocyte colony stimulating factor ( G CSF ) , interleukin 3 ( IL 3 ) , interleukin 6 ( IL 6 ) , stem cell factor ( SCF ) , erythropoietin ( EPO ) , insulin like growth factor 1 ( IGF 1 ) , basic fibroblast growth factor ( FGF b ) and ( B ) combination A plus FLT 3 ligand ( FL ) and megakaryocyte growth and development factor ( PEG rhMGDF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
These include endothelin ( ET ) 1 , granulocyte macrophage colony stimulating factor , membrane type stem cell factor ( SCF ) and growth related oncogene alpha for interactions between keratinocytes and melanocytes , and hepatocyte growth factor and soluble type SCF for interactions between fibroblasts and melanocytes . ^^^ These are ET 1 / ET B receptor as well as membrane type SCF / c KIT for ultraviolet B melanosis , granulocyte macrophage colony stimulating factor for ultraviolet A melanosis , ET 1 / ET B receptor as well as membrane type SCF for lentigo senilis , growth related oncogene alpha for Riehl ' s melanosis , sphingosylphosphorylcholine for hyperpigmentation in atopic dermatitis , ET 1 for seborrhoeic keratosis , soluble type SCF as well as hepatocyte growth factor for dermatofibroma and caf au lait macules , and c KIT for vitiligo vulgaris . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
This study has compared the expression of endothelin ( ET ) 1 , the ET 1 receptor ( ET ( B ) R ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , stem cell factor ( SCF ) , the SCF receptor ( KIT protein ) , tyrosinase , and S 100 alpha between lesional and non lesional vitiligo epidermis . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Noncontact HUBEC cultures and the addition of thrombopoietin , stem cell factor ( SCF ) , and macrophage colony stimulating factor 1 receptor ( Fms ) like tyrosine kinase 3 ( Flt 3 ) ligand supported further increases in CD 34 ( + ) CD 38 cells ( 6 . 4 fold and 13 . 1 fold ) , which correlated with significant increases in SRC activity . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Different concentrations of CD 34 ( + ) cells were grown in gas permeable Teflon bags with different serum free and serum containing media supplemented with GM CSF , IL 4 , TNF alpha , SCF , Flt 3L and INF alpha . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In this study , the effect of hematopoietic cytokines , i . e . , granulocyte colony stimulating factor ( G CSF ) , stem cell factor ( SCF ) , and granulocyte macrophage colony stimulating factor ( GM CSF ) , on renal function was studied in cisplatin induced acute renal failure in mice . ^^^ There was no significant change observed among these parameters following treatment with SCF or with GM CSF . ^^^ In conclusion , G CSF , but not SCF or GM CSF , acts to accelerate regeneration and prevent apoptosis of renal tubular epithelial cells and leads to reduced renal injury in cisplatin induced acute renal failure in mice . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The nature of UCBC was freshly determined and then UCBC were cultured for 1 , 2 , 3 and 4 weeks with granulocyte monocyte colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) , recombinant human stem cell factor ( SCF ) and EPO . ^^^ UCBC cultured for 1 , 2 , 3 and 4 weeks with GM CSF , IL 3 , EPO and SCF were shown to differentiate into CD1a+ CD11c+ CD83+ CDw123+ DC . ^^^ It is concluded that the mononuclear cells of UCB are able to differentiate into CD1a+ , CD83+ , CD11c+ and CDw123+ DC when UCBC are cultured with proper cytokines of GM CSF , SCF , EPO and IL 3 for 2 4 weeks . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In addition , this fraction differentially regulated liver and lung cytokine mRNA levels , resulting in significant increases in message for hematopoietic cytokines [ granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) ] . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , granulocyte colony stimulating factor ( G CSF ) and macrophage colony stimulating factor ( M CSF ) are members of a group of glycoproteins called hematopoietic cytokines ( HCs ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
By means of flow cytometry , CD34+ / CD38 hematopoietic stem cells ( HSC ) were collected from umbilical cord blood ( UCB ) of 10 healthy women at the time of delivery and cultivated in stem cell culture media supplemented with cell growth stimulating factors ( IL 3 , IL 6 , GM CSF , EPO , IGF 1 , and SCF ) for long periods . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We show here that exogenous Emi 1 is unstable in CSF arrested Xenopus eggs and is destroyed by the SCF ( betaTrCP ) ubiquitin ligase , suggesting that endogenous Emi 1 , an apparent 44 kDa protein , requires a stabilizing factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
RESULTS : USSC constitutively produced SCF , LIF , TGF 1beta , M CSF , GM CSF , VEGF , IL 1beta , IL 6 , IL 8 , IL 11 , IL 12 , IL 15 , SDF 1alpha , and HGF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
A novel human stem cell factor ( SCF ) / macrophage colony stimulating factor ( M CSF ) fusion protein gene was constructed , in which the coding regions of human SCF cDNA ( 1 165aa ) and the truncated M CSF cDNA ( 1 149aa ) were connected by a linker sequence encoding a short peptide GGGGSGGGGSGG . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The purpose of this study was to investigate the radioprotective effect of HGFs ( GM CSF , IL 3 and SCF ) in irradiated human peripheral blood mononuclear cells ( PBMCs ) in vitro , and the survival effect of lethally irradiated C3H mice in vivo . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Late MSCs also exhibited attenuated synthesis of the hematopoietic cytokines granulocyte colony stimulating factor ( G CSF ) , leukemia inhibitory factor ( LIF ) , and stem cell factor ( SCF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In PSBS SCM , the levels of IL 6 , IL 17 , G CSF and GM CSF were markedly increased , but not those of IL 3 and SCF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Cytokine and growth factor genes , including IL 6 , IL 8 , IL 11 , IL 12 , IL 14 , IL 15 , LIF , G CSF , GM CSF , M SCF , FL and SCF , were found to be expressed in the MSCs . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The effects of cytokines such as TPO , GM CSF and SCF and of a low dose of H 202 on the transport activity and its kinetic parameters are compared . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Qualitatively , BB gelatin induced secretion of KC , MIP 2 , MCP 1 , RANTES , and MIP 1a as well as IL 6 but not 6Ckine , CTACK , Eotaxin , G CSF , GM CSF , IL 2 , 3 , 4 , 5 , 9 , 10 , 12 , 13 , 17 , Leptin , IFN gamma , SCF , sTNFri , TARC , TNF alpha , TIMP 1 , Tpo , and VEGF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Stem cell factor ( SCF ) and macrophage colony stimulating factor ( M CSF ) can act in synergistic way to promote the growth of mononuclear phagocytes . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
OBJECTIVES : We investigated whether granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) could promote myocardial regeneration after coronary artery occlusion and improve left ventricular ( LV ) function . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Therefore , in the present study , we investigated in pancreatic cancer patients the serum levels of selected HCs , such as stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , granulocyte colony stimulating factor ( G CSF ) and macrophage colony stimulating factor ( M CSF ) in relation to a control group and to a group of patients with chronic pancreatitis . ^^^ There were significant differences in the levels of circulating SCF , IL 3 , GM CSF , M CSF , CEA and CA 19 9 in the pancreatic cancer patients compared to the control group . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Granulocyte colony stimulating factor ( G CSF ) , alone or in combination with stem cell factor ( SCF ) , can improve hemodynamic cardiac function after myocardial infarction . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The APC / C inhibitor Emi 2 or XErp 1 has recently been characterized as a pivotal CSF component , required to maintain metaphase 2 arrest and rapidly destroyed in response to Ca ( 2+ ) signaling through phosphorylation by Plx 1 and ubiquitination by the SCF ( betaTrCP ) complex . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In addition , mitogenic cytokines SCF , IL 3 , and GM CSF induced colony formation of K 562 cells that could be inhibited by PD 98059 or enhanced by SB 203580 , respectively , indicating that these mitogenic cytokines induce K 562 colony formation by activating ERK and inactivating p 38 MAPK . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
After surgically excising established LLC tumors , mice received at the excision site gelatin sponge implants containing VEGF to chemoattract CD 34 ( + ) cells , and / or GM CSF plus SCF to induce CD 34 ( + ) cell differentiation into dendritic cells . ^^^ Implants containing VEGF , GM CSF / SCF or VEGF / GM CSF / SCF had a higher proportion of CD 34 ( + ) cells compared to control implants . ^^^ However , the number of dendritic cells was higher in implants containing GM CSF / SCF or VEGF / GM CSF / SCF than those containing either VEGF or diluent . ^^^ Regional lymph node from mice containing GM CSF / SCF or VEGF / GM CSF / SCF implants showed increased dendritic cell levels . ^^^ However , when lysates from LLC ( GFP ) were added to the implants , the highest proportion of dendritic cells associated with GFP was in lymph nodes of mice containing GM CSF / SCF implants . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
BACKGROUND : Hematopoietic cytokines , granulocyte colony stimulating factor ( G CSF ) , and stem cell factor ( SCF ) were reported to show a neuroprotective effect or to support neurogenesis . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
OBJECTIVE : The aims of this study were , first , to determine if granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) improved left ventricular function in the setting of a reperfusion model of myocardial infarction ( MI ) and , second , to evaluate the effects of G CSF / SCF on cellular repair and , in particular , the fate of bone marrow cells homing to the site of tissue injury . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
RESULTS : The optimal compositions of serum substitutes and the cytokine cocktail for HSC expansion in the MNC culture system were BIT ( 4 g / L BSA , 0 . 71 microg / mL insulin , and 27 . 81 microg / mL transferrin ) , and CC 9 ( 5 . 53 ng / mL TPO , 2 . 03 ng / mL IL 3 , 16 ng / mL SCF , 4 . 43 ng / mL FL , 2 . 36 ng / mL IL 6 , 1 . 91 ng / mL G CSF , 1 . 56 ng / mL GM CSF , 2 . 64 ng / mL SCGF , and 0 . 69 ng / mL IL 11 ) in the Iscove ' s modified Dulbecco ' s medium . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
These conditions include : the combinations and concentrations of hematopoietic growth factors ( stem cell factor [ SCF ] , granulocyte macrophage colony stimulating factor [ GM CSF ] , interleukin [ IL ] 3 , thrombopoietin [ Tpo ] , IL 6 and Fms like tyrosine kinase 3 ligand [ Flt 3L ] ) , the duration of culture , and the effect of serum supplementation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Tryptase also induced the expression of mRNA for GM CSF and SCF , two cytokines known to promote growth of AML cells , in cultured bone marrow fibroblasts . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Lyn is renowned for its role in B cell antigen receptor and FcepsilonRI signaling ; however , it is becoming increasingly apparent that Lyn also functions in signal transduction from growth factor receptors including the receptors for GM CSF , IL 3 , IL 5 , SCF , erythropoietin , CSF 1 , G CSF , thrombopoietin and Flt 3 ligand . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CSFs included stem cell factor ( SCF ) , erythropoietin ( EPO ) , granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , macrophage colony stimulating factor ( M CSF , CSF 1 ) , and thrombopoietin ( TPO ) , or analogues of these . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Expansion of umbilical cord blood mononuclear cells ( UCB MNCs ) was carried out in a rotating wall vessel ( RWV ) bioreactor and tissue culture flasks ( T flasks ) in serum containing medium supplemented with relatively low doses of purified recombinant human cytokines ( 5 . 33 ng / ml IL 3 , 16 ng / ml SCF , 3 . 33 ng / ml G CSF , 2 . 13 ng / ml GM CSF , 7 . 47 ng / ml FL and 7 . 47 ng / ml TPO ) for 8 days . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Most large animal studies have utilized peripheral blood or marrow derived cells collected after administration of granulocyte colony stimulating factor ( G SCF ) and stem cell factor ( SCF ) ; however , SCF is unavailable for clinical use in the United States and the European Union . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Wild type ( WT ) mice underwent a 30 minute coronary occlusion followed by reperfusion and received vehicle , granulocyte colony stimulating factor ( G CSF ) +Flt 3 ligand ( FL ) , G CSF+stem cell factor ( SCF ) , or G CSF alone starting 4 hours after reperfusion . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
METHODS : CD34+ cells were seeded at 1 10 10 ( 5 ) / mL and expanded for 14 days in RPMI + 10 % autologous plasma supplemented with GM CSF , IL 4 , Flt 3L and SCF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
This cell line , designated OCI / AML1a , does not respond to GM CSF , interleukin 3 ( IL 3 ) , IL 1 or stem cell factor as well as G CSF . ^^^ This cell line TMD 2 does not respond to G CSF , GM CSF , IL 1 , or stem cell factor and anti IL 3 antibody blocks the stimulatory effect of IL 3 on these cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The receptors for at least two hematopoietic growth factors , namely the stem cell factor and colony stimulating factor 1 , belong to class 3 receptor tyrosine kinases . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
OBJECTIVE : Cytokine treatment with granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and stem cell factor ( SCF ) is a mainstay of current and future clinical and research protocols for peripheral blood stem cell mobilization , therapeutic care after hematopoietic stem cell transplantation ( HSCT ) , and ex vivo hematopoietic stem and progenitor cell ( HSC / HPC ) expansion . ^^^ We set out to establish whether short term in vitro G CSF , GM CSF , or SCF treatment upregulates CD 26 and thereby has a detrimental effect on the chemotactic potential of HSC / HPC that could be reversed by CD 26 inhibitor treatment . ^^^ MATERIALS AND METHODS : CD34+ or CD34+CD38 cells , a population enriched in HSC , were isolated from human umbilical cord blood and subjected to G CSF , GM CSF , or SCF treatment . ^^^ RESULTS : Treatment with G CSF and GM CSF but not SCF upregulates CD 26 expression and activity resulting in a CD 26 inhibitor reversible downregulation of CXCL 12 induced chemotactic response . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Granulocyte colony stimulating factor ( G CSF ) and stem cell factor ( SCF ) are potential new therapies to ameliorate post myocardial infarction ( post MI ) remodeling , as they enhance endogenous cardiac repair mechanisms and decrease cardiomyocyte apoptosis . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In cultures of TRL 01 , addition of GM CSF , SCF , and G CSF and adhesion to fibronectin coated plates promoted transient proliferation and survival , although they did not support long term culture . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Response to the cytokines SCF , IL 3 , and GM CSF was determined by this assay for the leukemic mouse strain BXH 2 and ten other inbred mouse strains . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We have investigated the influence of different hematopoietic growth factors , including granulocyte colony stimulating factor ( G CSF ) , stem cell factor ( SCF ) , Flt 3 ligand ( Flt 3L ) and thrombopoietin ( TPO ) , on the course of relapsing experimental autoimmune encephalomyelitis , a mouse model of multiple sclerosis . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
While thrombopoietin ( TPO ) , granulocyte colony stimulating factor ( G CSF ) or stem cell factor ( SCF ) were not able to increase cAMP levels , the antiapoptotic activity exerted by these growth factors was blocked by inhibition of the adenylate cyclase and synergized by BIMPS . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In agar culture of post 5 fluorouracil mouse bone marrow cells ( FUBM ) , recombinant rat stem cell factor ( rrSCF ) synergizes with granulocyte colony stimulating factor ( G CSF ) , interleukin 3 ( IL 3 ) or interleukin 6 ( IL 6 ) to stimulate primitive progenitor cells ( HPP CFCs ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Effect of stem cell factor with and without granulocyte colony stimulating factor on neonatal hematopoiesis : in vivo induction of newborn myelopoiesis and reduction of mortality during experimental group B streptococcal sepsis . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Analysis of the NK cell supernatants using factor dependent myeloid progenitor cell lines showed that the NK cells were producing G CSF and growth promoting activity that could not be attributed to IL 1 , IL 3 , IL 4 , IL 5 , IL 6 , GM CSF , G CSF , macrophage CSF ( M CSF ) , or stem cell factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Recombinant human stem cell factor synergises with GM CSF , G CSF , IL 3 and epo to stimulate human progenitor cells of the myeloid and erythroid lineages . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Genetic and structural homology of stem cell factor and macrophage colony stimulating factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In 6 of 11 cases that expressed c kit product , AML cells were found to proliferate in response to recombinant human stem cell factor ( rhSCF ) , the ligand for c kit , and the synergistic stimulation of AML cells was observed by rhSCF and granulocyte macrophage colony stimulating factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Members of the receptor tyrosine kinase family bind dimeric forms of macrophage colony stimulating factor , stem cell factor and platelet derived growth factor leading to kinase activation and phosphorylation of many substrates involved in production of second messengers . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Clonal analysis of bone marrow and spleen cells suggested that CEE may contain the avian equivalents of stem cell factor , interleukin 3 , granulocyte macrophage CSF , granulocyte CSF , and macrophage CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Culturing of CD34+ cells for 14 days with interleukin ( IL ) 1 , IL 3 , IL 6 , stem cell factor , granulocyte macrophage colony stimulating factor ( GM CSF ) , and G CSF resulted in on average a 1 , 750 fold amplification of cell number , of which 83 % + / 7 % were MPO+ . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In tritiated thymidine incorporation assays , stem cell factor plus granulocyte macrophage colony stimulating factor stimulated thymidine incorporation in normal CD34+ cells was reduced to 72 % of control values in the presence of MIP 1 alpha , whereas incorporation by CML CD34+ cells exposed to the same factors was not altered . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We have generated immunodeficient scid / scid ( SCID ) transgenic mice expressing the genes for human interleukin 3 , granulocyte / macrophage colony stimulating factor , and stem cell factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Recombinant granulocyte macrophage colony stimulating factor ( rGM CSF ) , recombinant granulocyte CSF ( rG CSF ) , recombinant macrophage CSF ( rM CSF ) , rIL 6 , rIL 11 , and recombinant stem cell factor ( rSCF ) did not alone expand nonerythroid progenitor cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Ligand induced dimerization is a key step in the activation of receptor tyrosine kinases , including the epidermal growth factor receptor , stem cell factor receptor ( c kit ) , and colony stimulating factor 1 receptor ( c fms ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Raf 1 is a necessary component of the mitogenic response of the human megakaryoblastic leukemia cell line MO 7 to human stem cell factor , granulocyte macrophage colony stimulating factor , interleukin 3 , and interleukin 9 . ^^^ We have examined the role of Raf 1 in the mitogenic response of the factor deprived human megakaryoblastic leukemia cell line MO 7 to recombinant human granulocyte macrophage colony stimulating factor , interleukin 3 , interleukin 9 , and stem cell factor by using c raf antisense oligodeoxyribonucleotides . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Mobilization of long term hematopoietic reconstituting cells in mice by the combination of stem cell factor plus granulocyte colony stimulating factor . ^^^ In this study , we have compared the ability of recombinant human granulocyte colony stimulating factor ( rhG CSF ) alone and the combination of low doses of recombinant rat pegylated stem cell factor ( rrSCF PEG ) plus rhG CSF to mobilize peripheral blood progenitor cells ( PBPCs ) with long term engrafting potential . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In vivo synergy between recombinant human stem cell factor and recombinant human granulocyte colony stimulating factor in baboons enhanced circulation of progenitor cells . ^^^ Recombinant human stem cell factor ( rhSCF ) and recombinant human granulocyte colony stimulating factor ( rhG CSF ) are synergistic in vitro in stimulating the proliferation of hematopoietic progenitor cells and their precursors . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Proliferation of ELF 153 cells was highly stimulated by interleukin 3 ( IL 3 ) and granulocyte macrophage colony stimulating factor and to a lesser extent by stem cell factor and IL 6 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Single factors such as IL 3 , IL 6 , GM CSF , and stem cell factor did not optimally stimulate growth of cord blood CFU GM . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Using this system , CD34+ cells isolated from bone marrow were cultured in the presence of GM CSF , G CSF , erythropoietin , stem cell factor , IL 1 , IL 3 , and IL 6 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Proliferative capacity was measured by generation of nascent myeloid progenitor cells ( granulocyte macrophage colony stimulating factor ; CFU GM ) and nucleated cells in a stroma free liquid culture stimulated by a combination of six hematopoietic growth factors ( interleukin 1 ( IL 1 ) , IL 3 , IL 6 , GM CSF , G CSF , and stem cell factor ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In this preliminary experience we found that , in vitro , rhEPO potentiates the effect of rhG CSF on HP growth and differentiation toward the granulocyte macrophage lineage . rhEPO plus rhG CSF produced in vitro a proliferative stimulus of HP which represents 26 % of the maximum stimulation obtained using IL 1 , IL 3 , IL 6 , G CSF , GM CSF and stem cell factor in combination . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Proliferation assay using a variety of cytokines demonstrated that the HSM 911 cells proliferate in response to recombinant granulocyte macrophage colony stimulating factor ( rGM CSF ) , recombinant Interleukin 3 ( rIL 3 ) and recombinant stem cell factor ( rSCF ) , but do not in response to recombinant granulocyte colony stimulating factor ( rG CSF ) , natural macrophage colony stimulating factor ( M CSF ) , rIL 1 , rIL 2 , rIL 4 , rIL 5 , rIL 6 or recombinant erythropoietin ( rEpo ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
ELISA and bioassays showed that stem cell factor and interleukin 6 were produced by the HUCB stromal cell cultures , but interleukin 3 or granulocyte / macrophage colony stimulating factor was not detected . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Mutual inhibition of murine erythropoiesis and granulopoiesis during combined erythropoietin , granulocyte colony stimulating factor , and stem cell factor administration : in vivo interactions and dose response surfaces . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Rapid engraftment by peripheral blood progenitor cells mobilized by recombinant human stem cell factor and recombinant human granulocyte colony stimulating factor in nonhuman primates . ^^^ We have previously shown that administration of low dose recombinant human stem cell factor ( rhSCF ) plus recombinant human granulocyte colony stimulating factor ( rhG CSF ) to baboons mobilizes greater numbers of progenitor cells in the blood than does administration of rhG CSF alone . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Maximal numbers of granulocyte monocyte progenitors in mononuclear cell cultures , could be achieved with a mixture of stem cell factor , interleukin 3 and granulocyte monocyte colony stimulating factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Flow cytometric analysis of five myeloma cell lines ( RPMI 8226 , ARH 77 , KMM 1 , U 266 , and Hs ) and myeloma cells freshly isolated from eight patients showed that interleukin 1 receptor ( IL 1R ) type 1 and type 2 , IL 2R alpha and beta chains , IL 4R , IL 6R , IL 7R , IL 8R , granulocyte macrophage colony stimulating factor receptor ( GM CSFR ) , c kit ( stem cell factor receptor [ SCFR ] ) , membrane bound stem cell factor ( MBSCF ) , and tumor necrosis factor ( TNF ) receptors type 1 and type 2 were not always detected on the myeloma cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Cells were cultured in a suspension system for 3 days in the presence of stem cell factor and interleukin 3 ( IL 3 ) , after which granulocyte colony stimulating factor ( G CSF ) was added . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Polymerase chain reaction analysis of Saka cell RNA detected expression of messenger RNAs for interleukin 6 ( IL 6 ) , IL 1 beta , granulocyte macrophage colony stimulating factor , macrophage colony stimulating factor , stem cell factor , and the 1 , 25 dihydroxyvitamin D 3 receptor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
For instance , leukemia , inhibitor factor and stem cell factor act on pluripotent cells and immature progenitors , granulocyte macrophage colony stimulating factor ( GM CSF ) acts at early stages of the development of myelomonocytic lineage , whereas granulocyte colony stimulating factor ( G CSF ) and macrophage colony stimulating factor ( M CSF ) act on more mature cells of the same lineage and are only required later during the differentiation of this cell lineage . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The use of powerful combinations of newer growth factors , such as interleukin 3 with granulocyte macrophage colony stimulating factor ( GM CSF ) , or granulocyte colony stimulating factor ( G CSF ) with stem cell factor , may obviate the need for chemotherapy . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Peripheral blood progenitor cells mobilized by recombinant human granulocyte colony stimulating factor plus recombinant rat stem cell factor contain long term engrafting cells capable of cellular proliferation for more than two years as shown by serial transplantation in mice . ^^^ Transplantation of recombinant human granulocyte colony stimulating factor ( rhG CSF ) plus recombinant rat stem cell factor ( rrSCF ) mobilized PBPC resulted in 98 % donor engraftment of primary recipients at 12 to 14 months post transplantation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Stem cell factor and interleukin 6 , alone or in combination with granulocyte colony stimulating factor , do not affect the growth of solid tumor cell lines . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Hematopoietic progenitor assays for unseparated ( cell concentration 3 10 10 ( 4 ) , 1 10 10 ( 5 ) , 3 10 10 ( 5 ) ) and CD 34 enriched cells ( cell concentration 1 10 10 ( 3 ) , 3 10 10 ( 3 ) , 1 10 10 ( 4 ) ) were performed in the presence of 5 U human interleukin 3 ( hu IL 3 ) , 30 U hu IL 6 , 10 U human granulocyte colony stimulating factor , 6 U erythropoietin , and 15 ng human stem cell factor . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Culture of isolated CD 34 cells in liquid culture with interleukin 3 , stem cell factor , G CSF , and granulocyte macrophage GM CSF showed over a 100 fold expansion in cell numbers after 25 days , with the peak expansion of colony forming cells occurring between days 11 and 16 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We conclude from these findings that PHSCs are programmed to interact with stem cell factor , IL 3 , and IL 6 but not with granulocyte or macrophage colony stimulating factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
This defect is characterized by suppression of a subpopulation of IL 3 responsive cells and ablation of stem cell factor synergy with GM CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In contrast to the vigorous proliferation in vivo , CML N 1 cells from any passage failed to proliferate in vitro under standard liquid culture conditions with or without growth factors , such as granulocyte macrophage colony stimulating factor , granulocyte colony stimulating factor , monocyte colony stimulating factor , interleukin 3 , interleukin 6 and stem cell factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Canine MGDF is characterized as a human megakaryocyte colony stimulating factor that acts synergistically with human recombinant stem cell factor but not interleukin 3 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Furthermore , rhTPO induced proliferation of AML cells was augmented with the addition of interleukin 3 ( IL 3 ) , IL 6 , stem cell factor , or granulocyte macrophage colony stimulating factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
OBJECTIVE : To determine fetal serum and amniotic fluid ( AF ) levels of interleukin ( IL ) 3 , IL 6 , granulocyte macrophage colony stimulating factor , stem cell factor , and erythropoietin , and to explore the relationship between cytokines and hemoglobin concentration , white blood cell count ( WBC ) , and platelet count in fetuses affected by Rh immunization . ^^^ The levels of IL 3 , IL 6 , granulocyte macrophage colony stimulating factor , stem cell factor , and erythropoietin were estimated using commercially available immunoassays . ^^^ Fetal serum contained higher concentrations of IL 3 , granulocyte macrophage colony stimulating factor , stem cell factor , and erythropoietin compared with AF . ^^^ Other studied cytokines ( such as stem cell factor , granulocyte macrophage colony stimulating factor , and IL 6 ) did not correlate with the degree of fetal anemia . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
TM 8 cells expressed certain levels of macrophage colony stimulating factor and stem cell factor messenger RNAs ( mRNAs ) , but low levels of c fms mRNA . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Recombinant cytokines including GM CSF , G CSF , IL 1 beta , IL 6 , stem cell factor , LIF , erythropoietin , and IL 3 had no effect on P gp expression whereas TNF alpha induced dose and time dependent P gp and mdr 1 gene overexpression . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The SCID hu mice were treated with a cytokine mixture ( recombinant human stem cell factor , interleukin 3 , granulocyte / macrophage colony stimulating factor , and granulocyte colony stimulating factor ) for 4 months and were then maintained for further 8 months under cytokine free conditions . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Granulocyte macrophage colony forming cells ( GM CFC ) are bipotential progenitor cells that can proliferate and develop into macrophages in response to macrophage CSF or into neutrophils in response to stem cell factor or granulocyte CSF . ^^^ Calphostin C also blocked IL 4 mediated development of macrophages in stem cell factor and granulocyte CSF treated cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
FSDC stimulated poorly allogeneic or syngeneic T cells in the primary mixed leukocyte reaction , and markedly increased this function after treatment with GM CSF , GM CSF and interleukin ( IL ) 4 or interferon gamma ( IFN gamma ) ; in contrast , stem cell factor , IL 1 alpha and tumor necrosis factor alpha had no effect . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The response of B cell precursors to the cytokine interleukin 7 is variably affected while that of several other cytokines ( stem cell factor , interleukin 3 , GM CSF , G CSF , and erythropoietin ) is normal in c abl mutants . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Recombinant stem cell factor , recombinant granulocyte macrophage colony stimulating factor , and catalase were also analyzed by TGGE , indicating that the technique can be used to analyze denaturation of monomeric and multimeric proteins . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Stem cell factor enhances in vivo effects of granulocyte colony stimulating factor for stimulating mobilization of peripheral blood progenitor cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Furthermore , soluble c kit ligand / rh stem cell factor ( rhSCF ) , in vitro in combination with rhIL 3 and rhGM CSF or PIXY 321 , increased the size but not the number of colonies derived from recovery phase BFU Meg and CFU Meg . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Severe congenital neutropenia : abnormal growth and differentiation of myeloid progenitors to granulocyte colony stimulating factor ( G CSF ) but normal response to G CSF plus stem cell factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The comparative study of the cytokine gene expression in MS 5 and in the nonsupportive cell lines included Northern blot and reverse transcriptase polymerase chain reaction analysis of messenger RNA for interleukin 1 , 3 , 6 , granulo macrophage colony stimulating factor ( GM CSF ) , granulocyte CSF , macrophage CSF , stem cell factor , transforming growth factor beta , tumor necrosis factor alpha , macrophage inflammatory protein 1 alpha , and leukemia inhibitory factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Consistent gene expression in the BM of all 7 individuals was detected for macrophage colony stimulating factor ( CSF ) , stem cell factor , interleukin 6 ( IL 6 ) , IL 7 , erythroid potentiating factor , erythroid differentiating factor , and insulinlike growth factor 1 , all cytokines with reported direct stimulatory effects on in vitro hematopoiesis . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In addition , CML and normal stromal supernatants contained similar quantities of both growth stimulatory ( granulocyte colony stimulating factor ( CSF ) , interleukin 6 , stem cell factor , granulocyte macrophage CSF , and interleukin 1 beta ) and growth inhibitory cytokines ( transforming growth factor beta , macrophage inflammatory protein 1 alpha , and tumor necrosis factor alpha ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We therefore searched for immediate early cytokine responsive genes and isolated a novel gene , CIS ( cytokine inducible SH 2 containing protein ) which is induced in hematopoietic cells by a subset of cytokines including interleukin 2 ( IL 2 ) , IL 3 , granulocyte macrophage colony stimulating factor ( GM CSF ) and erythropoietin ( EPO ) , but not by stem cell factor , granulocyte colony stimulating factor and IL 6 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Purified CD 34 positive hematopoietic progenitors were uPAR negative , but expressed uPAR during differentiation in short term liquid culture stimulated in vitro by recombinant interleukin ( IL ) 1 , IL 3 , IL 6 , granulocyte macrophage colony stimulating factor ( CSF ) , granulocyte CSF , and stem cell factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
A new serum free culture ( SFC ) system for human AML CFU was established and the colony promoting activity of four recombinant human hematopoietic growth factors ( rhHGFs ) including granulocyte macrophage colony stimulating factor ( rhGM CSF ) , interleukin 3 ( rhIL 3 ) , erythropoietin ( rhEPO ) and newly developed stem cell factor ( rhSCF ) were investigated in this SFC system . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In both colony stimulating factor 1 and stem cell factor stimulated cells , there is a marginal increase in tyrosine phosphorylation of HCP . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Culture supernatants from blasts cultured with or without TPA showed the production of large amounts of IL 8 , IL 6 , TNF alpha , MIP 1 alpha , IL 10 and interferon gamma and modest amounts of IL 1 alpha , GM CSF and stem cell factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
To identify the possible factors responsible for the activities detected in the keratinocyte conditioned media , we performed northern blot analysis using the cytokine probes granulocyte colony stimulating factor , macrophage colony stimulating factor , granulocyte macrophage colony stimulating factor , stem cell factor , interleukin 1 alpha , interleukin 3 , and tumor necrosis factor alpha . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Allergen inhalation increased airway responsiveness ( P < 0 . 001 ) and the number of granulocyte macrophage colony forming units ( CFU ) when cultured with dog serum and either recombinant canine stem cell factor ( rcSCF ) ( P < 0 . 001 ) or granulocyte colony stimulating factor ( rcG CSF ) ( P = 0 . 035 ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
After vector exposure , optimal gene transfer was noted when CML mononuclear cell cultures contained stem cell factor , IL 3 , GM CSF and erythropoietin . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
With tumor necrosis factor ( TNF ) + granulocyte macrophage colony stimulating factor ( GM CSF ) + stem cell factor treatment numerous colony like clusters developed . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Supplementation of serum free medium with recombinant cytokines , including stem cell factor , granulocyte macrophage colony stimulating factor ( GM CSF ) , and tumor necrosis factor ( TNF ) alpha or with human placenta conditioned medium ( HPCM ) matched the degree of inhibition of apoptosis induced by BMF in only 50 % of cases . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Early and late normal progenitor / stem cells were studied in the presence of placenta conditioned medium ( placenta conditioned medium granulocyte macrophage colony forming units ) ; in the presence of granulocyte colony stimulating factor , granulocyte macrophage colony stimulating factor , interleukin 3 , erythropoietin , stem cell factor ( 5R CFU GM ) ; stimulated granulocyte macrophage colony forming and in a long term culture initiating cell assay . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Reverse transcriptase polymerase chain reaction showed that IL 3 , IL 4 , IL 6 , stem cell factor , granulocyte colony stimulating factor , and granulocyte / macrophage colony stimulating factor mRNA expression were higher in 15 deoxyspergualin treated chimeras than in control chimeras , indicating that these cytokines are responsible for an enhancement of hematopoiesis . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Although neither stem cell factor , nor GM CSF alone can significantly induce the proliferation of stem cells , the combination induces the proliferation of these cells . ^^^ Committed progenitor cells such as granulocyte macrophage colony forming cells , however , are stimulated to proliferate by GM CSF alone , while stem cell factor in combination with GM CSF results in only a slight additive effect . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Nor is Stat 4 activated in myeloid cells by a number of cytokines , including erythropoietin , IL 3 , granulocyte colony stimulating factor , stem cell factor , colon stimulating factor 1 , hepatocyte growth factor , IL 2 , IL 4 , and IL 6 . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
IL 3 used in conjunction with synergistically acting cytokines such as granulocyte macrophage colony stimulating factor ( GM CSF ) or granulocyte CSF ( G CSF ) have already yielded interesting results , while new factors like stem cell factor are in the process of clinical evaluations and appear promising . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
By various analyses , this protein was shown to be molecularly different from the heretofore identified cytokines that may affect megakaryocytopoiesis , i . e . , interleukin 1 ( IL 1 ) , IL 2 , IL 3 , IL 6 , IL 7 , IL 11 , granulocyte colony stimulating factor ( CSF ) , macrophage CSF , GM CSF , leukemia inhibitory factor , stem cell factor and tumor necrosis factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
This process was inhibited by granulocyte and granulocyte / macrophage colony stimulating factor , but not by interleukin 3 ( IL 3 ) , IL 6 , or stem cell factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The cell cycle kinetics of AML blast cells are susceptible to stimulation by a variety of cytokines , including granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin ( IL ) 3 , granulocyte colony stimulating factor and stem cell factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We show here that bone marrow myeloid progenitor cells from p 53 deficient mice are more resistant to induction of apoptosis when there was only a low concentration of the viability factors granulocyte macrophage colony stimulating factor ; interleukins 1 alpha , 3 , and 6 ; or stem cell factor ; or when apoptosis was induced in these cells by irradiation or heat shock . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Studies in which stem cell factor was used in combination with other growth factors showed that stem cell factor allowed the survival of stem cells , while a second growth factor ( granulocyte macrophage colony stimulating factor ) stimulated the stem cells to develop normally . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Interleukin 3 , granulocyte macrophage colony stimulating factor and stem cell factor play regulatory functions in the early steps of erythropoiesis . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Polymerase chain reaction analyses showed that most of the cell lines produce FLK 2 / FLT 3 ligand , stem cell factor , macrophage colony stimulating factor , leukemia inhibitory factor , and interleukin 6 ( IL 6 ) , whereas there is a generally low or not measurable production of granulocyte colony stimulating factor , granulocyte macrophage colony stimulating factor , IL 1 , IL 3 , transforming growth factor beta 1 , erythropoietin , or thrombopoietin . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We examined here how DCs differentiated from human cord blood CD34+ progenitor cells cultured with granulocyte macrophage colony stimulating factor , tumor necrosis factor alpha , and stem cell factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Furthermore the stimulatory effect of the P 1 fraction on DNA synthesis in human melanocytes was neutralized by antibodies to GM CSF , but not to basic fibroblast growth factor or stem cell factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Some promising mobilizing regimens include the use of stem cell factor and growth factors such as interleukin 3 , granulocyte colony stimulating factor , and granulocyte macrophage colony stimulating factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
To investigate humoral inhibitory activities we examined the response of 15 DBA bone marrows in vitro to the inhibitory chemokine macrophage inflammatory protein 1 alpha ( MIP 1 alpha ) in the presence of the stimulatory cytokines erythropoietin , granulocyte macrophage colony stimulating factor , IL 3 , and stem cell factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We investigated the effects of granulocyte macrophage colony stimulating factor , interleukin 3 , stem cell factor , interleukin 6 , and granulocyte colony stimulating factor ( G CSF ) alone , and in combination , on the clonogenic potential of normal and aplastic anemia ( AA ) bone marrow mononuclear cells ( BMMC and CD34+ cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We have studied graft versus host disease ( GVHD ) after transplantation of allogeneic peripheral blood stem cells ( PBSC ) mobilized by either recombinant canine granulocyte colony stimulating factor ( rcG CSF ) alone or combined with stem cell factor ( rcSCF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Significantly , apoptosis of the cells occurred even in the presence of the survival factors granulocyte CSF and stem cell factor but was prevented by engagement of beta ( c ) with interleukin 3 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In particular , the following hematopoietic growth factors are discussed : erythropoietin , stem cell factor , interleukin 3 , interleukin 5 , granulocyte macrophage colony stimulating factor , granulocyte colony stimulating factor , and macrophage colony stimulating factor . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In contrast to spontaneous blast proliferation , in the presence of exogenous hematopoietic growth factors ( granulocyte colony stimulating factor , granulocyte macrophage colony stimulating factor , interleukin 3 , tumor necrosis factor alpha , stem cell factor ) , IL 1 inhibition caused either increased or decreased AML blast proliferation depending on the individual patient . ^^^ When AML blasts were cultured with stem cell factor plus granulocyte macrophage colony stimulating factor , IL 1 inhibition significantly increased AML blast proliferation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Moreover , mice receiving either granulocyte colony stimulating factor ( G CSF ) or G CSF+ stem cell factor before low dose TBI ( 160 cGy ) exhibited a marked increase in engraftment compared with mice receiving a vehicle control before low dose TBI ( 18 . 9 % and 20 . 6 % 5 5 . 6 % at a 1 month , respectively ; 29 % and 35 % 5 15 . 1 % at 4 months , respectively ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Effect of cytokine treatment ( granulocyte colony stimulating factor and stem cell factor ) on hematopoiesis and the circulating pool of hematopoietic stem cells in mice . ^^^ The mobilization of hematopoietic stem cells ( HSCs ) into the peripheral blood of mice was induced by recombinant human granulocyte colony stimulating factor ( rhG CSF ) ( 250 microgram / kg / d ) alone or combined with recombinant rat stem cell factor ( rrSCF ) ( 34 microgram / kg / d ) , injected subcutaneously ( s . c . ) once a day for 10 and 17 days . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Quantitative reverse transcriptase polymerase chain reaction analysis revealed that the mRNAs of macrophage colony stimulating factor and stem cell factor , but not that of Interleukin 3 , were detectable in all the hematopoietic supportive and non supportive stromal cell lines . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We have previously shown that normal CD34+ HPCs express high levels of fibronectin ( Fn ) receptors very late antigen 4 ( VLA 4 ) and VLA 5 in a low affinity state , which do not allow HPCs to strongly adhere on immobilized Fn , and that cytokines such as interleukin 3 , granulocyte monocyte colony stimulating factor , and stem cell factor transiently activate these receptors , providing HPCs with an adhesive phenotype on Fn . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
IL1RA release from AML blasts was also increased by stem cell factor , tumour necrosis factor alpha ( TNF alpha ) , granulocyte macrophage colony stimulating factor and macrophage colony stimulating factor , whereas interleukin 3 , interleukin 6 , leukaemia inhibitory factor and granulocyte colony stimulating factor did not significantly alter IL1RA release . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Human megakaryoblastic leukemia cell lines have common biological features : high expression of the megakaryocytic specific antigen ( CD 41 ) ; high expression of early myeloid antigens ( CD 34 , CD 33 and CD 13 ) ; constitutive expression of IL 6 and platelet derived growth factor ; a complex karyotype picture ; expression of c kit ( the stem cell factor receptor ) ; growth dependency or stimulation by IL 3 and / or GM CSF ; and in vivo tumorigenicity in mice associated with marked fibrosis . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Human erythroid progenitor cells were isolated from peripheral blood of healthy donors and amplified in a suspension culture system using recombinant growth factors ( stem cell factor , interleukin 3 , granulocyte macrophage colony stimulating factor and erythropoietin ) as well as conditioned medium from a human bone marrow stroma cell line to support cell proliferation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The level of alkyltransferase in CD34+ cells or in mononuclear BM cells did not increase after incubation with granulocyte macrophage colony stimulating factor , interleukin 3 , stem cell factor , the combination , or 5637 conditioned medium . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Growth of RA A progenitors in cytokines promoting myelopoiesis ( GMCSF , TNF , stem cell factor ) produced increased monocyte and dendritic cell progeny , in support of the flow cytometry data . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In five cases CD34+ selected cells grown in a stroma free long term bone marrow culture system with the addition of stem cell factor , interleukin 3 , interleukin 6 and GM CSF every 48 h , showed a 15 ( + / 15 ) and 31 ( + / 21 ) mean colony forming unit granulocyte / macrophage fold increase on cultures at days 7 and 14 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Furthermore , MKE acted synergistically with granulocyte colony stimulating factor ( CSF ) , macrophage CSF , IL 6 and IL 11 on colony formation , but did not act with GM CSF , stem cell factor and Epo . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Pluripotential hematopoietic stem cells are most influenced by IL 3 , GM CSF , and stem cell factor while committed progenitor cells are regulated by variable concentrations of GM CSF , G CSF , M CSF , IL 5 , Epo , and Tpo . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CD34+ cells isolated from bone marrow were cultured in iscove ' s modified Dulbecco ' s medium supplemented with 10 % fetal calf serum , 1 % bovine serum albumin , 50 ng / mL stem cell factor , 50 ng / mL interleukin 3 ( IL 3 ) , 50 ng / mL IL 6 , 100 ng / mL granulocyte colony stimulating factor , and 3 U / mL erythropoietin for 7 days . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
IL 1 beta , granulocyte macrophage colony stimulating factor , macrophage colony stimulating factor and stem cell factor increased IL 6 secretion , whereas IL 4 caused decreased IL 6 secretion together with increased release of IL 1 receptor antagonist . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The effects of interleukins 3 and 6 , stem cell factor , and granulocyte macrophage colony stimulating factor on human fetal hematopoietic , bone marrow , and cord blood cells were studied on the basis of the colony forming capacity . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
By assessing the formation of colonies , CD34+ enrichment was evaluated with and without cytokine stimulation ( interleukins 3 and 6 , stem cell factor , granulocyte macrophage colony stimulating factor ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CD34+ purified cells , were cultured in suspension with 6 combined hematopoietic growth factors ( IL1beta , IL 3 , IL 6 at 100 U / ml and G CSF , GM CSF and stem cell factor at 10 ng / ml of each ) for up to four weeks . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Here , we show that the sequential use of early acting hematopoietic growth factors , stem cell factor , interleukin ( IL ) 3 , and IL 6 , followed on day 8 by differentiation in the two factor combination IL 4 plus granulocytemacrophage colony stimulating factor ( GM CSF ) ( CC4GM ) is more efficient and allows the cells to be arrested in the LC stage for more than 1 week while continuous maturation occurs in CC 7 7 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Nonadherent mononuclear bone marrow cells were incubated for 8 days in the presence of the serum and recombinant canine hemopoietic cytokines ( stem cell factor , granulocyte colony stimulating factor , GM colony stimulating factor ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CD1a+ dendritic cells ( DC ) differentiate from a major population of nonadherent CD 13 ( hi ) lin cells that appear when human cord blood CD34+ hematopoietic progenitor cells are cultured with stem cell factor , granulocyte / macrophage ( MA ) colony stimulating factor , and tumor necrosis factor alpha ( TNF alpha ) for 5 days . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The supernatants of primary cell and BMEC 1 contain stem cell factor , interleukin 6 ( IL 6 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , IL 1 alpha , IL 11 , and G CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
After retroviral transduction , human CD34+ cells were differentiated into DCs in vitro using granulocyte macrophage colony stimulating factor , tumor necrosis factor alpha , and stem cell factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Platelet coated cells decreased dramatically after several days of incubation in a serum free medium supplemented with stem cell factor , IL 3 , IL 6 , and / or GM CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We found that a combination of epidermal growth factor ( EGF ) , the OP 9 stromal cell line , which lacks macrophage colony stimulating factor , recombinant stem cell factor , and the chemically defined medium mSFO 2 provides a microenvironment where c Kit+ Thy 1+ / lo Mac 1+ / lo B 220 TER 119 common beta + IL 2R gamma + gp130+ cells are selectively propagated from normal , unfractionated bone marrow cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Retroviral transduction of human progenitor cells : use of granulocyte colony stimulating factor plus stem cell factor to mobilize progenitor cells in vivo and stimulation by Flt3 / Flk 2 ligand in vitro . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The myeloid cytokines granulocyte colony stimulating factor , granulocyte monocyte colony stimulating factor , interleukin 3 and stem cell factor enhanced the adhesion of AML blast cells to BMFs in some cases . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Furthermore , the TPO induced proliferation of AML cells was augmented by the addition of the other hematopoietic growth factors such as interleukin 3 ( IL 3 ) , IL 6 , stem cell factor , or granulocyte macrophage colony stimulating factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Moreover , we found that CD 44 mediated adhesion of CD34+ cells to HA could be enhanced by phorbol 12 myristate 13 acetate ( PMA ) , the function activating anti CD 44 monoclonal antibody H 90 , and cytokines such as granulocyte monocyte colony stimulating factor , interleukin 3 ( IL 3 ) , and stem cell factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Several other factors have a direct ( interleukin 3 , 6 , 11 , 13 , GMCSF , erythropoietin ) or indirect ( interleukin 1 , stem cell factor ) positive effect . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The enriched population of CD34+ cells ( more than 95 % ) was cultured in the presence of stem cell factor , interleukin 3 ( IL 3 ) , IL 6 , and either granulocyte colony stimulating factor or macrophage colony stimulating factor to achieve granulocyte or monocyte / macrophage differentiation , respectively . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CD34+ cells were first expanded with the growth factors granulocyte macrophage CSF and stem cell factor to generate a population of intermediate progenitor cells with a relatively immature phenotype . ^^^ To induce specific differentiation to dendritic cells , the cultures were switched to serum free medium with the growth factors granulocyte macrophage CSF , stem cell factor , TNF alpha , and IL 4 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We showed that even a complex cocktail of HGFs ( stem cell factor , interleukin 3 , IL 6 , IL 11 , granulocyte colony stimulating factor , and erythropoietin ) is unable to induce significant myeloid differentiation in day 12 embryoid bodies . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CD34+ cells were cultured in liquid medium in the presence of interleukin 3 , granulocyte macrophage colony stimulating factor . stem cell factor , granulocyte colony stimulating factor and a powerful proliferation with prevalent differentiation along the granulocytic / monocytic lineage was obtained . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Recombinant human granulocyte macrophage colony stimulating factor ( CSF ) , but not recombinant human granulocyte CSF , down regulates the recombinant human stem cell factor dependent differentiation of human fetal liver derived mast cells . ^^^ The effects of recombinant human granulocyte CSF ( rhG CSF ) and recombinant human granulocyte macrophage CSF ( rhGM CSF ) on the recombinant human stem cell factor ( rhSCF ) dependent development of human mast cells from fetal liver progenitors were examined . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
By contrast , M CSF ( 1000 U / ml ) , GM CSF ( 100 ng / ml ) , IL 1 , IL 3 , IL 6 and stem cell factor ( 10 ng / ml ) did not stimulate PAF production . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Delayed addition of a cytokine cocktail ( cytokines ; interleukin ( IL ) 3 , IL 6 , stem cell factor , erythropoietin , granulocyte macrophage colony stimulating factor , and TPO ) to cultures with TPO alone on day 7 induced various colonies including granulocyte macrophage ( GM ) colonies , erythroid bursts ( E ) , granulocyte erythrocyte macrophage megakaryocyte ( GEMM ) colonies . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The addition of FL to serum free cultures of CD34+ cells supplemented with TGF beta 1 , granulocyte macrophage colony stimulating factor , tumor necrosis factor alpha , and stem cell factor strongly increases both percentages ( mean , 36 % + / 5 % 5 64 % + / 4 % ; P = . 001 ) and total numbers ( 4 . 4 + / 0 . 8 fold ) of CD1a+ dendritic cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In an increasing number of hematopoietic cytokine receptor systems ( T cell receptor , B cell receptor , and macrophage colony stimulating factor , stem cell factor , interleukin 3 , and erythropoietin [ EPO ] receptors ) , inhibitory roles for the protein tyrosine phosphatase hematopoietic cell phosphatase ( HCP ; SHPTP 1 , PTP1C , and SHP 1 ) have been defined in proliferative signaling . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Cytokines that were upregulated included interleukin 1alpha ( IL 1alpha ) and 1beta , IL 1 receptor antagonist , IL 6 , IL 11 , granulocyte colony stimulating factor , granulocyte macrophage colony stimulating factor , macrophage colony stimulating factor , stem cell factor , lymphotoxin beta , transforming growth factor beta 1 , and tumor necrosis factor alpha . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CFU O was enriched in a nonadherent , low density , lineage marker negative ( Lin ) , Thy1 . 2 negative ( Thy1 . 2 ) , Sca 1 negative ( Sca 1 ) , and c kit positive ( c kit+ ) population , as were the progenitors that were responsive to macrophage colony stimulating factor ( CSF ; CFU M ) , granulocyte macrophage CSF ( CFU GM ) , and stem cell factor ( CFU SCF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
There were no differences as to protein production of the extracellular matrix , production of granulocytic macrophagal colony stimulating factor , stem cell factor , transforming growth factor beta , interleukin 1 beta and interleukin 6 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Eotaxin activity synergized with stem cell factor but not with interleukin 3 or granulocyte macrophage colony stimulating factor and was inhibited by pertussis toxin . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The system sustained extensive erythropoiesis at low erythropoietin concentrations ( 0 . 2 U / mL ) , plus stem cell factor , interleukin 3 , granulocyte macrophage colony stimulating factor , and insulin like growth factor 1 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
These studies demonstrate for the first time that ( 1 ) mouse oval cells respond to a wide variety of GDFs including various members of the interleukin , chemokine , stem cell factor , EGF , FGF , PDGF , TGF beta , VEGF , insulin , CSF , TNF , HGF , and IFN growth and differentiation factor families in addition to multiple comitogens and ( 2 ) during tumorigenic conversion mouse oval cells undergo alterations which result in both alterations in GDF responsiveness and the autocrine / paracrine production of multiple GDFs . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Hematopoietic stem cells , CD 34 positive cells , were isolated from the peripheral blood of three patients with malignant lymphoma , and were cultivated in suspension for 14 days in the presence of cytokines , including granulocyte colony stimulating factor , granulocyte macrophage colony stimulating factor , interleukin 3 and stem cell factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Interestingly , AS E 2 cells are strictly dependent on EPO for their growth and survival ; other cytokines including GM CSF , stem cell factor , or IL 3 can not support the growth of this cell line . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Low dose stem cell factor , 25 micrograms / kg / d , that does not elicit a detectable biological response , enhances the effects of granulocyte colony stimulating factor in vivo , increasing the neutrophilia and circulation of progenitor and marrow repopulating cells above that which is achieved with either factor alone . ^^^ Phase 1 2 trials of low dose stem cell factor in combination with granulocyte colony stimulating factor show that the combination increases the circulation of CD34+ cells and colony forming progenitor cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The growth response of colony forming cells to varying concentrations of defined growth factors ( interleukin 3 , interleukin 6 , granulocyte macrophage colony stimulating factor , stem cell factor , and erythropoietin ) was determined , as well as the frequency of burst forming units erythroid , colony forming units granulocyte macrophage , and total colony forming cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Moreover , SNAP 23 was induced in response to a wide variety of cytokines , including IL 2 , IL 3 , IL 5 , IL 10 , stem cell factor , G CSF , GM CSF , leukemia inhibitory factor , and erythropoietin . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
PU . 1 / myeloid progenitors can proliferate in vitro in response to the multilineage cytokines interleukin 3 ( IL 3 ) , IL 6 and stem cell factor but are unresponsive to the myeloid specific cytokines granulocyte macrophage colony stimulating factor ( GM CSF ) , G CSF and M CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In order to analyze these effects we studied the levels of interleukin 3 ( IL 3 ) , interleukin 6 , granulocyte macrophage colony stimulating factor , erythropoietin , stem cell factor , soluble vascular adhesion molecule 1 , soluble intercellular adhesion molecule 1 , soluble E selectin and soluble P selectin in 7 SS patients before and during 5 months of HU treatment . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Hematopoietic cytokines ( stem cell factor , interleukin 3 , granulocyte macrophage colony stimulating factor [ GM CSF ] , and granulocyte colony stimulating factor [ G CSF ] contribute to upregulation of CD 95 on bone marrow cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Target cells in this assay were CML cells that were cultured in the presence of stem cell factor , interleukin 3 , granulocyte macrophage colony stimulating factor , granulocyte colony stimulating factor , and erythropoietin . [ 3H ] thymidine incorporation at day 7 represented the proliferation of the progeny of the CD 34 ( + ) CML progenitor cells , and not of the more mature CD 34 ( ) CML cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
GM CFC will form neutrophils in the presence of the cytokines stem cell factor and granulocyte colony stimulating factor , whereas macrophage colony stimulating factor leads to macrophage formation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Culture of these isolated CD 34 ( + ) CD 33 ( lo ) thymic progenitors with a mixture of cytokines , including interleukin 7 ( IL 7 ) , IL 1alpha , IL 6 , granulocyte macrophage colony stimulating factor , and stem cell factor , results in predominant generation of DCs . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
A combination of stem cell factor and granulocyte colony stimulating factor enhances the growth of human progenitor B cells supported by murine stromal cell line MS 5 . ^^^ Intriguingly , the addition of recombinant human stem cell factor ( rhSCF ) and granulocyte colony stimulating factor ( rhG CSF ) , but not rhIL 7 , strikingly enhanced the growth of progenitor B cells from CB CD34+ population cultured on MS 5 stromal cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In the present study , abnormal hematologic profiles were induced with erythropoietin ( EPO ) , recombinant murine stem cell factor ( rm SCF ) , granulocyte macrophage stimulating factor ( GM CSF ) , and cyclophosphamide ( CP ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
By reverse transcription polymerase chain reaction , the expression by MMHs of cytokines involved in the survival and self renewal of early progenitor cells ( stem cell factor and FLT 3 ligand ) as well as those acting at different stages of progenitor differentiation ( interleukin [ IL ] 1beta , IL 3 , leukemia inhibitory factor , IL 6 , granulocyte macrophage colony stimulating factor , granulocyte colony stimulating factor , macrophage colony stimulating factor , and thrombopoietin ) was shown . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Additional neutralizing antibody studies suggest that MDA is not one of the cytokines known to induce some degree of megakaryopoiesis in vitro or in vivo including interleukin 3 ( IL 3 ) , IL 6 , IL 11 , granulocyte macrophage colony stimulating factor , erythropoietin , or stem cell factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Cord blood CD34+ cells were cultured with granulocyte macrophage colony stimulating factor ( GM CSF ) and tumor necrosis factor alpha ( TNF alpha ) , to which stem cell factor and Flt 3 ligand were added for 5 days . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Among a panel of growth regulatory factors tested ( granulocyte macrophage colony stimulating factor [ GM CSF ] , IL 3 , granulocyte colony stimulating factor , stem cell factor , and insulin like growth factor 1 ) GM CSF was the only molecule for which both an inhibition of spontaneous BFU E formation by its respective antibody as well as a significant restimulation of erythroid colonies in IL 10 treated cultures by exogenous addition was found . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Because stem cell factor , interleukin 6 ( IL 6 ) , and oncostatin M , but not IL 3 , IL 11 , leukemia inhibitory factor , granulocyte colony stimulating factor , granulocyte macrophage colony stimulating factor , thrombopoietin , and Flk 2 ligand were detected in reverse transcription polymerase chain reaction analysis of AGM S 3 cells , the cells seem to express species cross reactive molecule ( s ) other than the cytokines examined and which act on primitive hematopoietic progenitor / stem cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Replating potential of colony forming units of granulocytes / macrophages ( CFU GM ) expanded ex vivo by stem cell factor , interleukin ( IL ) 3 , IL 6 , granulocyte colony stimulating factor , erythropoietin with or without thrombopoietin . ^^^ CD34+ cells isolated from cord blood were cultured in Iscove ' s modified Dulbecco ' s medium supplemented with 10 % fetal bovine serum , 1 . 0 % bovine serum albumin , 50 ng / ml stem cell factor , 50 ng / ml interleukin 3 ( IL 3 ) , 50 ng / ml IL 6 , 100 ng / ml granulocyte colony stimulating factor , and 3 U / ml erythropoietin for 0 , 5 , 7 , 10 , 14 , and 21 days . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We here report that the in vitro proliferative potential of r metHuSCF ( stem cell factor ) plus filgrastim ( granulocyte colony stimulating factor ; r metHuG CSF ) mobilized peripheral blood ( PB ) CD 34 ( + ) cells obtained from previously heavily treated non Hodgkin ' s lymphoma patients inversely correlates with extent of prior therapy . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Prolonged release and c kit expression of haemopoietic precursor cells mobilized by stem cell factor and granulocyte colony stimulating factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
After 6 7 days of culturing the CD34+ cells of HNSCC patients with granulocyte macrophage colony stimulating factor , stem cell factor , and tumor necrosis factor at , there appeared CD14+CD1a+ and a lesser proportion of CD 14 ( ) CD1a+ cells resembling the precursor cells of the bipotential and committed dendritic cell differentiation pathways that have been described for cord blood CD34+ cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
DCs were generated ex vivo in the presence of tumor necrosis factor alpha , granulocyte macrophage colony stimulating factor , stem cell factor , and flk 2 / flt 3 ligand taken from CD34+ hematopoietic progenitors that were mobilized into the peripheral blood of cancer patients treated with high dose cyclophosphamide and filgrastim . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The results show that using a growth factor combination including granulocyte macrophage colony stimulating factor plus tumor necrosis factor alpha plus interleukin 4 plus stem cell factor plus Flt 3 ligand , more than 70 % of DCs derived from CB or MPB CD34+ cells can be transduced . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
To examine whether progenitor homing was altered upon ex vivo expansion , highly enriched Sca 1 ( + ) c kit+Lin cells were cultured for 9 days in serum free medium containing interleukin ( IL ) 6 , IL 11 , granulocyte colony stimulating factor , stem cell factor , flk 2 / flt3 ligand , and thrombopoietin . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In vitro growth of myeloid leukemic cell lines and of blasts from 14 primary AMLs demonstrated that recombinant human leptin alone induced low level proliferation , significantly ( P < . 05 ) increased proliferation induced by recombinant human granulocyte colony stimulating factor , interleukin 3 , and stem cell factor in a subset of AML and increased colony formation ( P < . 005 ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The addition of growth factors such as G CSF , GM CSF , IL 3 , IL 6 , or Stem Cell Factor did not affect the growth . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Co expression of CD 7 on CD34+ cells was induced to decrease significantly after short term in vitro culture with the differentiation inducing agent phorbol ester ( PMA ) and with a combination of cytokines ( stem cell factor , interleukin 3 and granulocyte colony stimulating factor ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The c mpl ligand , thrombopoietin ( TPO ) , is a physiologic regulator of platelet and megakaryocytic production , acting synergistically on thrombopoiesis with the growth factors interleukin 11 ( IL 11 ) , stem cell factor , interleukin 3 ( IL 3 ) , interleukin 6 ( IL 6 ) , and granulocyte macrophage colony stimulating factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Although adenoviruses can infect nondividing cells , we observed that a combination of growth factors ( GM CSF , IL 3 , stem cell factor ) was required for efficient transduction in order to maintain AML blast cell viability . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
When fresh or thawed cells are cultured with granulocyte macrophage colony stimulating factor and stem cell factor with or without tumour necrosis factor alpha ( TNF alpha ) , they generate numerous cells with the characteristic ultrastructural , phenotypic and functional properties of dendritic cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In a second step , we established three different cytokine combinations by adding the megakaryocyte growth and development factor + / erythropoietin and granulocyte colony stimulating factor to a fetal calf serum containing medium with Flt 3 , stem cell factor , and interleukin 3 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
However , the cellular growth rate was significantly accelerated in the presence of various growth factors such as granulocyte colony stimulating factor , stem cell factor , granulocyte macrophage colony stimulating factor , interleukin 3 , and thrombopoietin . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In 2 animals , cells of the different fractions were transduced in the presence of human FLT 3 ligand ( FLT3L ) , canine stem cell factor ( cSCF ) , and human megakaryocyte growth and development factor ( MGDF ) , and in 2 other dogs , transduction was performed in the presence of FLT3L , cSCF , and canine granulocyte colony stimulating factor ( cG CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
SVZ neural precursor cells could not be generated with platelet derived growth factor ( PDGF ) , granulocyte macrophage colony stimulating factor , stem cell factor , heparin binding epidermal growth factor ( HB EGF ) , granulocyte colony stimulating factor , or ciliary neurotrophic factor ( CNTF ) , but could be with EGF , fibroblast growth factor 2 ( FGF 2 ) , and FGF 2 plus heparin . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CIS 1 is induced by interleukin 2 ( IL 2 ) , IL 3 , GM CSF , and erythropoietin ( EPO ) , but not by IL 6 , granulocyte colony stimulating factor ( G CSF ) , or stem cell factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The colonies grown from progenitor cells were cultured in methylcellulose either in the presence or absence of growth factors , including GM CSF , stem cell factor , and IL 3 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Single CD34+ cells from the CD 123 subsets were cultured in a cytokine cocktail of stem cell factor , interleukin 3 ( IL 3 ) , IL 6 , GM CSF , erythropoietin , insulin like growth factor 1 , and basic fibroblast growth factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We have generated DC from human umbilical cord blood CD34+ progenitors cultured with granulocyte macrophage colony stimulating factor , tumor necrosis factor alpha ( TNF alpha ) , and stem cell factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Colonies grown from progenitor cells cultured in methylcellulose were determined for 14 days in the presence or absence of growth factors , including granulocyte monocyte colony stimulating factor , stem cell factor , and interleukin 3 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
A murine bone marrow stromal cell line , HESS 5 , supports the survival and proliferation of Lin ( ) CD 34 ( ) cells in the presence of fetal calf serum and human cytokines thrombopoietin , Flk 2 / Flt 3 ligand , stem cell factor , granulocyte colony stimulating factor , interleukin 3 , and interleukin 6 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In vitro proliferation and differentiation of cord blood derived CD 34 ( + ) cells ( induced by stem cell factor and granulocyte macrophage colony stimulating factor ) were consistently inhibited by the addition of anti p75 / AIRM1 mAb . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
METHODS : To obtain large numbers of pig hematopoietic cells , leukapheresis was used to collect blood cell products in miniature swine ( n = 5 ) after progenitor cell mobilization by use of a course of hematopoietic growth factors ( cytokines ) , consisting of porcine interleukin 3 , porcine stem cell factor , and human granulocyte colony stimulating factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We found that the cytokines stem cell factor ( maximal response with 1 mM ) , granulocyte colony stimulating factor ( 0 , 1 mM ) and erythropoietin ( 1 mM ) relaxed , while tumor necrosis factor alpha ( 0 . 1 mM ) , interleukin ( IL ) 6 ( 10 mM ) and IL 10 ( 0 . 1 mM ) induced contraction of arterial but not of venous segments . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We found no neutralization of granulocyte colony stimulating factor , macrophage colony stimulating factor , stem cell factor , IL 1beta , IL 2 , IL 3 , IL 4 , IL 6 , IL 9 , IL 10 , IL 12 , tumour necrosis factor alpha , oncostatin M ( OSM ) and IFN gamma . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Murine bone marrow cells were incubated at 1 % oxygen in liquid medium supplemented with stem cell factor , granulocyte colony stimulating factor , interleukin 6 and interleukin 3 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Interleukin 1 , interleukin 6 , granulocyte monocyte colony stimulating factor , stem cell factor , scatter factor / hepatocyte growth factor and transforming growth factor beta , added separately to MCF 7 / AZ cells , could not mimic the effects of CM COLO 16 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : The effects of pH AND pO 2 on granulocyte proliferation , differentiation , and granulocyte colony stimulating factor receptor ( G CSFR ) expression were evaluated using mobilized peripheral blood CD 34 ( + ) cells directed down the granulocytic pathway with stem cell factor , interleukin 3 , interleukin 6 , and G CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The highest yield of CD1a ( + ) / CD14 ( ) cells could be obtained with stem cell factor , transforming growth factor beta , tumor necrosis factor alpha , GM CSF , and FLT 3 ligand . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Furthermore , this intracellular marker of priming is selective for IL 5 related cytokines , in that it is observed following exposure to IL 5 and granulocyte macrophage colony stimulating factor but not to interferon gamma , stem cell factor , tumor necrosis factor alpha , or IL 4 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We have shown that the sequential use of early acting hematopoietic growth factors , stem cell factor , IL 3 and IL 6 , followed by differentiation with IL 4 and granulocyte macrophage colony stimulating factor allows the in vitro generation of large numbers of immature DCs from CD 34 ( + ) peripheral blood progenitor cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The addition of granulocyte macrophage colony stimulating factor to hyper IL 6 and stem cell factor was essential for the differentiation of expanded progenitor cells into antigen presenting cells capable of inducing a primary T cell response to soluble protein , which is a typical feature of dendritic cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : CD 34 ( + ) PBPC were cultured in serum free medium comparing different ( n = 32 ) combinations of stem cell factor ( S ) , interleukin 1 ( 1 ) , interleukin 3 ( IL 3 ) ( 3 ) , interleukin 6 ( 6 ) , erythropoietin ( E ) , granulocyte colony stimulating factor ( G ) , granulate macrophage colony stimulating factor ( GM ) , daniplestim ( D , a novel IL 3 receptor agonist ) , and Flt 3 ligand ( FL ) under various culture conditions . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Such cytokines included granulocyte macrophage colony stimulating factor , granulocyte colony stimulating factor , interleukin 3 , and stem cell factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
BACKGROUND AND OBJECTIVE : Hepatocyte growth factor ( HGF ) is known to augment the effects of stem cell factor , interleukin 3 , granulocyte macrophage colony stimulating factor ( GM CSF ) , erythropoetin , and granulocyte colony stimulating factor , all of which are involved in hematopoiesis . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Purified CD34+ haematopoietic progenitor cells were cultivated with stem cell factor , interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) and granulocyte CSF ( G CSF ) for 7 d , and thereafter non adherent cells were divided into two groups . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Lin ( ) CD 34 ( ) population isolated from human cord blood was cultured in the presence of murine bone marrow stroma cell line , HESS 5 , and human cytokines , thrombopoietin , Flk2 / Flt3 ligand , stem cell factor , granulocyte colony stimulating factor , interleukin 3 ( IL 3 ) , and IL 6 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Cytokines that appear to have a functional role in mammalian implantation include leukaemia inhibitory factor , interleukin 1 , hepatocyte growth factor , stem cell factor , macrophage colony stimulating factor and insulin like growth factors . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We demonstrate here that LC development from human CD34+ hemopoietic progenitor cells in response to TGF beta 1 costimulation ( basic cytokine combination GM CSF plus TNF alpha , stem cell factor , and Flt 3 ligand ) is associated with pronounced cell cluster formation of developing LC precursor cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
To determine whether cytokine induced proliferation also limits the rate of short term engraftment and potential clinical utility of ex vivo expanded hematopoietic cells , murine Sca 1 ( + ) c kit ( + ) Lin ( ) cells were cultured in interleukin 6 ( IL 6 ) , IL 11 , granulocyte colony stimulating factor ( G CSF ) , stem cell factor , flk 2 ligand , and thrombopoietin for 7 days . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Moreover , addition of any of the hematopoietic cytokines , erythropoietin , stem cell factor , granulocytic colony stimulating factor , or granulomonocytic colony stimulating factor induced a highly significant increase in tube formation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Addition of other cytokines , including interleukin 3 ( IL 3 ) , IL 6 , granulocyte macrophage colony stimulating factor , and stem cell factor , to LIF in the cultures caused a 150 fold expansion of cells retaining the CD 34 ( + ) thy 1 ( + ) phenotype . ^^^ Furthermore , we have ruled out the contribution of 6 known early acting cytokines , including interleukin 3 , interleukin 6 , granulocyte macrophage colony stimulating factor , stem cell factor , flt 3 ligand , and thrombopoietin , to this SCEPF activity . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Compared with hMC maintained in stem cell factor alone , hMC primed with IL 5 expressed 2 to 4 fold higher steady state levels of TNF alpha , IL 5 , IL 13 , macrophage inflammatory protein 1alpha , and granulocyte macrophage colony stimulating factor transcripts 2 h after FcvarepsilonRI crosslinking and secreted 2 to 5 fold greater quantities of the corresponding cytokines , except IL 13 , at 6 h . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The CD 34 ( + ) cells were then transduced in virus containing medium ( gibbon ape leukemia virus pseudotype vector LAPSN [ PG 13 ] ) in wells coated with the recombinant human fibronectin fragment CH 296 and in the presence of multiple hematopoietic growth factors ( interleukin 6 , stem cell factor , granulocyte colony stimulating factor , granulocyte macrophage colony stimulating factor , and megakaryocyte growth and development factor ) and protamine sulfate . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Unselected PBPC were cultured in gas permeable bags containing 1 L serum free media , granulocyte colony stimulating factor , stem cell factor , and pegylated megakaryocyte growth and development factor for 9 days . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The concentrations of stem cell factor , interleukin 3 , interleukin 6 , erythropoietin , and granulocyte colony stimulating factor ( G CSF ) were measured using a commercially available immunoassay . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In cultures of human bone marrow cells stimulated with macrophage colony stimulating factor and stem cell factor , interleukin 6 inhibited colony formation by 51 74 % . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
To determine the frequency and responsiveness to cytokines of megakaryocyte progenitors ( CFU Meg ) in TAR syndrome , the authors studied marrow samples from 3 patients and 6 normal controls , using optimally standardized megakaryocyte growth media incorporating interleukin 3 , interleukin 6 , stem cell factor , and granulocyte monocyte colony stimulating factor , with and without pegylated recombinant human megakaryocyte growth and development factor ( PEG rHuMGDF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
METHODS : Immunodeficient transgenic mice were developed by crossing transgenic founders carrying porcine interleukin 3 , granulocyte macrophage colony stimulating factor , and stem cell factor genes with severe combined immunodeficient mice or non obese diabetic / severe combined immunodeficient mice . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Reassessment of interactions between hematopoietic receptors using common beta chain and interleukin 3 specific receptor beta chain null cells : no evidence of functional interactions with receptors for erythropoietin , granulocyte colony stimulating factor , or stem cell factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
MKE did not affect the total number of colonies of erythroid burst forming units ( BFU E ) , granulocyte macrophage colony forming units ( CFU GM ) or granulocyte erythroid macrophage megakaryocyte colony forming units ( CFU Mix / CFU GEMM ) in a methylcellulose culture with exogenous recombinant human granulocyte colony stimulating factor , granulocytemacrophage colony stimulating factor , interleukin 3 , stem cell factor and erythropoietin . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CD34 ( + ) cord blood ( CB ) cells were cultured in serum free medium containing stem cell factor , Flt 3 ligand , megakaryocyte growth and development factor , and granulocyte colony stimulating factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
G CSF , IL 6 and thrombopoietin ( TPO ) each enhanced light scatter compared to control PRP , although IL 3 , GM CSF , erythropoietin and stem cell factor did not . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In vivo priming of target cells , i . e . mobilization of HSC into the circulation with granulocyte colony stimulating factor and stem cell factor , as well as optimization of the in vitro transduction conditions , now allow a stable in vivo gene transfer efficiency of up to 10 15 % in both lymphoid and myeloid circulating cells in non human primates , levels that would be adequate for many clinical applications . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We discuss recent data indicating that engagement of p75 / AIRM1 or CD 33 sharply inhibits the in vitro proliferation / differentiation of CD34+ myeloid precursors induced by stem cell factor and granulocyte macrophage colony stimulating factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Exposure of HUVECs to ethanol ( 0 . 01 % 1 % ) dose dependently inhibited ( by 12 % 27 % ) the release of stem cell factor , granulocyte macrophage and granulocyte colony stimulating factors ( CSFs ) , or interleukin ( IL ) 8 , but not of macrophage CSF triggered by lipopolysaccharide ( LPS ) or IL 1 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Results of this study indicate that expression of vascular cell adhesion molecule 1 ( VCAM 1 ) is strongly reduced in vivo in the BM during HPC mobilization by granulocyte colony stimulating factor ( G CSF ) and stem cell factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
To assess the hematopoietic defect involved in PNH , CD34+ CD59+ ( normal phenotype hematopoietic stem / progenitor ) and CD34+ CD 59 ( PNH phenotype ) cells from PNH patients ( n = 16 ) and CD34+ CD59+ cells from healthy volunteers ( n = 10 ) were sorted as single cells into 96 well flat bottom culture plates containing culture medium supplemented with stem cell factor , interleukin ( IL ) 3 , erythropoietin , granulocyte macrophage colony stimulating factor ( GM CSF ) , G CSF , IL 6 , thrombopoietin , and Flt 3 ligand . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The present study was undertaken to : ( 1 ) compare the effect of some hematopoietic growth factors , like interleukine 3 , thrombopoietin , granulocyte megakaryocyte colony stimulating factor , stem cell factor , and reactive oxygen species such as H ( 2 ) O ( 2 ) on glucose uptake in a human leukemic megakaryocytic cell line , M 07 ; ( 2 ) investigate the changes in kinetic parameters of the transport activity induced by these stimuli ; and ( 3 ) evaluate the effect of genistein , a tyrosine kinase inhibitor , on the glucose uptake activation by the cited agents . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We treated human cord blood CD34+ cells with either GM CSF alone or in combination with stem cell factor and followed methylation at this locus using bisulfite genomic sequencing . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Granulocyte macrophage CSF , stem cell factor , interleukin 3 , and vascular endothelial growth factor could not similarly replace the need for M CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Stem cell factor could cooperate with G CSF , GM CSF , and erythropoietin significantly increasing the numbers of CFU GM , BFU E and CFU E of CAA , SAA and normal control ' s enriched CD 34 positive MNC . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In conclusion we have discovered a new function of the hemopoietic stem cell factor GM CSF , which is also a powerful arteriogenic peptide that acts via prolongation of the life cycle of monocytes / macrophages . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Apart from granulocyte macrophage CSF ( GM CSF ) , there have as yet been no reports of lithium salts inducing early activating factors for the megakaryocytic lineage , such as IL 3 , IL 11 , stem cell factor and flt 3 ligand , or maturation factors , such as thrombopoietin ( TPO ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : In severely hypoxic ( 0 . 9 % O ( 2 ) ) murine bone marrow cell cultures containing stem cell factor , interleukin 6 , and granulocyte colony stimulating factor , pre CFC maintenance was characterized by the culture repopulating ability assay , an in vitro analogue of the marrow repopulating ability assay . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Cord blood CD 34 ( + ) cells were cultured for 3 , 7 or 10 d with stem cell factor , Flt 3 , thrombopoietin , interleukin 3 ( IL 3 ) , IL 6 and granulocyte colony stimulating factor , all at 10 ng / ml in serum replete conditions . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Samples of the patient ' s bone marrow showed trilineage hypoplasia with absence of myeloid , erythroid , and megakaryocyte progenitor cells but with elevated endogenous levels of erythropoietin , granulocyte colony stimulating factor , and stem cell factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Purified CD34+ cells from CB and PB were cultured on murine stroma cell line MS 5 with stem cell factor and granulocyte colony stimulating factor for 6 weeks . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
PATIENTS AND METHODS : Unselected peripheral blood progenitor cells ( PBPCs ) from 43 breast cancer patients were seeded into gas permeable bags containing serum free media , granulocyte colony stimulating factor , stem cell factor , and pegylated megakaryocyte growth and development factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In this assay , unfractionated mononuclear cells from apheresis donors are incubated in nonpyrogenic plastic bags for 7 days in the presence of culture medium either containing fetal calf serum or human plasma , granulocyte colony stimulating factor , and stem cell factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Bone marrow CD34+ progenitor cells stimulated with stem cell factor and GM CSF have the capacity to activate IgD B cells through direct cellular interaction . ^^^ Bone marrow CD34+ cells from RA patients that had been stimulated with stem cell factor and GM CSF for 3 4 weeks ( > 90 % CD14+ HLA DR+ cells , < 0 . 5 % CD19+ B cells , and < 0 . 5 % CD3+ T cells ; MLC ) induced the production of IgG much more effectively than that of IgM by highly purified B cells from healthy donors in the presence of IL 2 and IL 10 . ^^^ These results indicate that upon stimulation with stem cell factor and GM CSF , CD34+ progenitor cells differentiate into MLC that activate preferentially IgD B cells through direct cellular interactions to produce IgG . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
MATERIAL AND METHODS : CD 34 ( + ) cells were selected from CB products and cultured in a two step procedure in the presence of stem cell factor , megakaryocyte growth and differentiation factor , and granulocyte colony stimulating factor for 14 days . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Although cytokines do not appear to be centrally involved in the pathogenesis of acute leukemias , leukemic cells express receptors for many of the cytokines regulating normal hematopoiesis , particularly G CSF , GM CSF , IL 3 , and stem cell factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Retroviral mediated expression of DokR in bone marrow cells dramatically inhibited their capacity to form colonies in vitro in response to the cytokines macrophage colony stimulating factor and stem cell factor , whereas responses to interleukin 3 and granulocyte macrophage colony stimulating factor were only weakly affected . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
TGD also enhanced secretion of granulocyte monocyte colony stimulating factor and stem cell factor but did not affect the production of erythropoietin , interleukin 3 , growth hormone , or of full length TPO . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Combinations of thrombopoietin and cytokines that act on megakaryocyte development ( stem cell factor , IL 3 , IL 6 , IL 11 , flt 3 ligand ( now known as FLT3LG ) , erythropoietin , GM CSF and G CSF were evaluated for their ability to enhance clonal growth in vitro of 10 irradiated CD 34 ( + ) megakaryocytic progenitor cells ( CFU megakaryocytes ) purified from normal human peripheral blood . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
A fraction of each patient ' s CB allograft was CD 34 selected and cultured ex vivo for 10 days prior to transplantation in defined media with stem cell factor , granulocyte colony stimulating factor , and megakaryocyte growth and differentiation factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Furthermore , when compared with WT , hematopoietic precursor cells from PLSCR 1 ( / ) mice showed defective colony formation and impaired differentiation to mature granulocytes as stimulated by stem cell factor and granulocyte colony stimulating factor ( G CSF ) . ^^^ Stem cell factor and G CSF were also found to induce marked increases in PLSCR 1 levels in WT cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Patients ( n = 69 ) with multiple myeloma undergoing peripheral blood stem cell collection ( PBSC ) were treated with cyclophosphamide and a combination of recombinant methionyl human granulocyte colony stimulating factor ( r metHuG CSF , filgrastim ) and recombinant methionyl human stem cell factor ( r metHuSCF , ancestim ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We investigated the role of three parameters : A ) the culture medium ( serum free versus serum dependent ) ; B ) the initial cell population ( Ficoll separated mononucleated cells versus CD 34 ( + ) selected cells ) , and C ) the low concentration of recombinant cytokines , flt 3 ligand , and thrombopoietin in association with a basic cocktail of stem cell factor , interleukin ( IL ) 6 , IL 3 , GM CSF , and erythropoietin . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We replaced granulocyte macrophage colony stimulating factor with macrophage colony stimulating factor from a serum free culture containing granulocyte macrophage colony stimulating factor , stem cell factor , Flt 3 ligand , tumor necrosis factor alpha , and transforming growth factor beta 1 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
For the work presented here , DC were differentiated from human peripheral blood mononuclear cells ( PBMC DC ) in culture medium containing GM CSF and interleukin ( IL ) 4 to ensure an immature phenotype or were derived from the KG 1 cell line ( KG 1 DC ) using a defined cytokine cocktail consisting of GM CSF , IL 4 , Flt 3 / Flk 2 ligand , thrombopoietin , stem cell factor , and tumor necrosis factor alpha ( TNFalpha ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Although Shiga toxin 2 alone induced colonies mainly composed of macrophages , it induced colonies mainly consisting of granulocytes when combined with physiological doses of interleukin 1beta , granulocyte colony stimulating factor , or stem cell factor with interleukin 3 . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Administration of granulocyte colony stimulating factor and stem cell factor resulted in mobilization of transduced bone marrow cells detectable as mature granulocyte progeny which expressed the GFP gene , suggesting that transduced progenitor cells in bone marrow could be mobilized into the peripheral blood and differentiated into granulocytes . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CD34+ peripheral blood progenitor cells were differentiated to immature DC in the presence of GM CSF , IL 6 / IL 6R fusion protein and stem cell factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
BAEP 2 SV40 cell line , but not BAEP 2 W , expressed interleukin ( IL ) 1 , IL 6 , granulocyte macrophage colony stimulating factor ( GM CSF ) , granulocyte colony stimulating factor , stem cell factor and vascular cell adhesion molecule 1 mRNAs , and secreted IL 6 and GM CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The usage of cytokines was classified into two groups : group A ( stem cell factor , granulocyte / macrophage colony stimulating factor , tumor necrosis factor alpha and interleukin 4 ) and group B ( granulocyte / macrophage colony stimulating factor , tumor necrosis factor alpha and interleukin 4 ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Alteration of spontaneous proliferation by at least one mediator was detected in five of 38 patients ; whereas , altered cytokine ( Flt 3 ligand , granulocyte macrophage colony stimulating factor , stem cell factor ) dependant proliferation was observed for 10 patients . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Successful stem cell mobilization with stem cell factor and granulocyte colony stimulating factor in patients with solid tumors failing conventional mobilization with chemotherapy and G CSF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CD 14 ( + ) monocytes and CD 34 ( + ) stem cells were isolated from human umbilical cord blood and were induced to differentiate into dendritic cells using 100 ng / mL granulocyte macrophage colony stimulating factor ( GM CSF ) , 25 ng / mL IL 4 , 2 . 5 ng / mL tumor necrosis factor alpha ( TNF alpha ) , 100 ng / mL GM CSF , 25 ng / mL stem cell factor , and 2 . 5 ng / mL TNF alpha , respectively . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Compared with prestimulated ( stem cell factor , granulocyte colony stimulating factor , granulocyte macrophage colony stimulating factor , interleukin 3 , interleukin 6 , erythropoietin , and monoclonal antibody to transforming growth factor beta ) , transfection of thawed nonstimulated PBSCs was equally efficient , with a median transfection rate of 3 . 7 % , transfection efficiency of 0 . 8 % , and survival of 19 . 5 % ( n = 5 ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Successful mobilization of peripheral blood stem cells after addition of ancestim ( stem cell factor ) in patients who had failed a prior mobilization with filgrastim ( granulocyte colony stimulating factor ) alone or with chemotherapy plus filgrastim . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Growth factors contributing to it , namely interleukin ( IL ) 6 , IL 11 , stem cell factor , leukemia inhibitory factor , granulocyte colony stimulating factor , thrombopoietin ( TPO ) , and the regulation of megakaryocytopoiesis during the inflammatory cascade are reviewed . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Progenitor cells from the blood of normal and asthmatic subjects were cultured for 14 days in methylcellulose with GM CSF , stem cell factor , IL 3 and IL 5 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
PERV was released from cultured porcine bone marrow cells without stimulation , and combinations of the porcine hematopoietic cytokines , interleukin 3 , granulocyte macrophage colony stimulating factor and stem cell factor had no additional effect on the infectivity or in vitro tropism of released PERV virions . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CD34+ cell derived hematopoietic precursors amplified with FLT 3 ligand , thrombopoietin and stem cell factor became , after a 6 day induction with GM CSF , IL 4 and TGF beta 1 , HLA DR+ , CD1a+ , CD 83 , CD 86 , CD 80 cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
RECENT FINDINGS : Several studies have recently shown that fibroblasts can modulate the functions of mast cells and eosinophils through the membrane form of stem cell factor and granulocyte macrophage colony stimulating factor , respectively . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Human umbilical cord blood CD14+ monocytes and CD34+ stem cells were induced to differentiate into dendritic cells using 100 ng / mL granulocyte macrophage colony stimulating factor ( GM CSF ) , 25 ng / mL interleukin ( 2 ) 4 , 2 . 5 ng / mL tumor necrosis factor alpha ( TNF alpha ) and 100 ng / mL GM CSF , 25 ng / mL stem cell factor , and 2 . 5 ng / mL TNF alpha , respectively . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
CD34+ stem cells were isolated from umbilical cord blood and cultured using granulocyte macrophage colony stimulating factor , stem cell factor , and varying concentrations of TNFalpha . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Most gene transfer studies have used peripheral blood or marrow repopulating cells collected after administration of granulocyte colony stimulating factor and stem cell factor ( G CSF / SCF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
To investigate the relationship between c kit expression and cell cycle regulation by endogenous transforming growth factor beta ( TGF beta ) in human bone marrow hematopoietic progenitor cells , CD34+ CD 38 c kit ( low / ) and CD34+ CD 38 c kit ( high ) populations were cultured in stem cell factor , thrombopoietin , interleukin 3 ( IL 3 ) , IL 6 , granulocyte colony stimulating factor , granulocyte / macrophage colony stimulating factor and anti TGF beta , and analyzed for cell cycle status . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Fresh leukaemic cells from five patients with acute myeloid leukaemia ( AML ) were isolated and retrovirally transduced with OX40L during the culture with a combination of cytokines from stem cell factor , fms like tyrosine kinase ( Flt ) 3 ligand , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 4 ( IL 4 ) and TNF alpha . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
RESULTS : We found that growth factors such as epidermal growth factor , transforming growth factor alpha , amphiregulin , heparin binding epidermal growth factor like growth factor , beta cellulin , insulin like growth factor 1 , insulin like growth factor 2 and stem cell factor stimulated directional ( chemotactic ) and / or random ( chemokinetic ) motility in all mesothelioma cell lines tested , whereas none of acidic fibroblast growth factor , basic fibroblast growth factor , granulocyte macrophage colony stimulating factor or interleukin 6 induced migration in the same mesothelioma cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Finally , in vitro proliferation , but not differentiation , of CD34+ cells cultured in the presence of fms like tyrosine kinase 3 ligand ( FLT3L ) , stem cell factor , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 , and G CSF is profoundly inhibited by treatment with CsA in a dose dependent manner . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Mice with targeted deletion of PLSCR 1 display perinatal granulocytopenia due to defective response of hematopoietic precursors to granulocyte colony stimulating factor and stem cell factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
LTA altered either cytokine ( granulocyte macrophage colony stimulating factor + stem cell factor + IL 3 ) dependent proliferation or the release of IL1beta / IL8 for 23 patients ; the effects were divergent but increased proliferation / cytokine levels were most commonly observed . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We cultured cord blood CD34+ cells in RPMI 1640 with 10 % human AB plasma , stem cell factor ( days 1 6 ) , granulocyte macrophage colony stimulating factor ( days 1 18 ) , interleukin 4 ( days 6 18 ) and varying schedules of TNF alpha ( 0 144 h after thawing ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The concentrations of interleukin 6 , stem cell factor , granulocyte monocyte colony stimulating factor , granulocyte colony stimulating factor , erythropoietin , and growth hormone ( GH ) were evaluated before and in the eight hours after exercise . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Similar to endothelial cells , myxoma cells did not produce stem cell factor , granulocyte colony stimulating factor , hepatocyte growth factor , or ET 3 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The cells were cultured in STEM PRO 34 liquid medium , supplemented with rHuEPO , IL 3 , stem cell factor , and granulocyte macrophage colony stimulating factor for 7 d and then transferred to a semisolid methylcellulose culture medium for performing burst forming unit erythroid ( BFU E ) colony assay . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
METHODS : CD34+ cells purified from the bone marrow of 13 patients with active RA and 9 control subjects ( 7 osteoarthritis [ OA ] patients and 2 healthy individuals ) were cultured in the presence of stem cell factor ( 10 ng / ml ) and granulocyte macrophage colony stimulating factor ( 1 ng / ml ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In addition , these infected cells were differentiated into immature and mature dendritic cells ( DCs ) using cytokine induction with recombinant human granulocyte macrophage colony stimulating factor ( rhGm CSF ) , recombinant human tumor necrosis factor ( rhTNF alpha ) and recombinant human stem cell factor ( rhSCF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
BM mononuclear cells from 3 dogs with melanoma and from 1 healthy dog were cultured for 12 days in media supplemented with recombinant human granulocyte macrophage colony stimulating factor , stem cell factor , tumor necrosis factor , and Flt 3 ligand . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
This study investigated whether the enhanced availability of hematopoietic stem cells , induced by stem cell factor and granulocyte colony stimulating factor , to the injured kidney provides an adequate strategy for stem cell based therapy to counteract renal ischemia / reperfusion injury . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Successful mobilization of peripheral blood stem cells using recombinant human stem cell factor in heavily pretreated patients who have failed a previous attempt with a granulocyte colony stimulating factor based regimen . ^^^ To assess the efficacy of recombinant human stem cell factor ( rHuSCF ) , 48 patients who had failed to mobilize > 2 . 0 10 10 ( 6 ) CD34+ cells / kg with granulocyte colony stimulating factor ( G CSF ) ( 10 microg / kg twice daily ) with , or without , concomitant chemotherapy ( G CSF based regimen ) , were remobilized with the addition of rHuSCF ( 20 microg / kg / day ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In order to permit cellular differentiation towards the myeloid lineage , PBPCs were cultured for 14 21 days in a liquid , serum free medium supplemented with interleukin 1 ( IL 1 ) , IL 3 , IL 6 , granulocyte macrophage colony stimulating factor , granulocyte colony stimulating factor and stem cell factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Subcutaneous administration of granulocyte colony stimulating factor and stem cell factor ameliorates the outcome of acute myocardial infarction . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
ES CM contained interleukin ( IL ) 1alpha , IL 10 , IL 11 , macrophage colony stimulating factor ( CSF ) , oncostatin M , stem cell factor , vascular endothelial growth factor , as well as a number of chemokines and other proteins , some of which are known to enhance survival / anti apoptosis of progenitors . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Ex vivo culture was performed in medium supplemented with granulocyte colony stimulating factor , stem cell factor and either thrombopoietin or megakaryocyte growth and differentiation factor . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Immature DCs are generated from human cord blood CD34+ hematopoietic stem / progenitor cells that were cultured in the presence of stem cell factor , Fms like tyrosine kinase 3 ligand , granulocyte macrophage colony stimulating factor , and transform growth factor beta 1 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Since primitive CML cells differentiate more rapidly in vitro than their normal counterparts and are also preferentially killed by mafosfamide and imatinib , we examined the purging effectiveness on CD 34 ( + ) CML cells using a strategy that combines a brief exposure to imatinib ( 0 . 5 1 . 0 microM for 72 h ) and then mafosfamide ( 30 90 microg / ml for 30 min ) followed by 2 weeks in culture with cytokines ( 100 ng / ml each of stem cell factor , granulocyte colony stimulating factor and thrombopoietin ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Silencing of NFkappaB 1 by small interfering RNA abrogated the capacity of RA bone marrow CD34+ cells to differentiate into fibroblast like cells and to produce MMP 1 and vascular endothelial growth factor upon stimulation with stem cell factor , granulocyte macrophage colony stimulating factor and TNF alpha without influencing their viability and capacity to produce beta 2 microglobulin . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
On the basis of changes in protein expression , we identified 6 cytokines that accurately discriminate between individuals on the basis of adverse event status : granulocyte colony stimulating factor , stem cell factor , monokine induced by interferon gamma ( CXCL 9 ) , intercellular adhesion molecule 1 , eotaxin , and tissue inhibitor of metalloproteinases 2 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
JMML arises from clonal expansion of a hematopoietic stem cell , and JMML cells and murine Nf 1 / hematopoietic cells are hypersensitive to granulocyte macrophage colony stimulating factor and KitL , the ligand for c kit . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Gonarthrosis in 11 human T cell leukemia virus 1 ( HTLV 1 ) carriers were studied for sera , synovial fluids ( SF ) and cerebrospinal fluids ( CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Entry into the cell cycle of dormant hematopoietic progenitors appears to be regulated by multiple synergistic factors , including interleukin 6 ( IL 6 ) , granulocyte colony stimulating factor ( G CSF ) , IL 11 , and the ligand for c kit , which is also known as steel factor ( SF ) . ^^^ In contrast , SF is unique among the synergistic factors in that it interacts either with growth factors such as IL 3 or GM CSF or with synergistic factors such as IL 6 , IL 11 , or G CSF . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Granulocyte macrophage colony stimulating factor ( GM CSF ) , Interleukin 3 ( IL 3 ) , and Steel Factor ( SF ) induce proliferation of hematopoietic cells through binding to specific , high affinity , cell surface receptors . ^^^ Although the proteins phosphorylated on tyrosine by GM CSF and IL 3 are similar or identical in MO 7 cells , many of the proteins that are phosphorylated on tyrosine after SF are different . ^^^ GM CSF , IL 3 , and SF were each found to induce MAP kinase activity when assayed in vitro using myelin basic protein ( MBP ) as a substrate . ^^^ GM CSF , IL 3 , and SF were each found to induce MAP kinase activity when assayed in vitro using myelin basic protein ( MBP ) as a substrate . ^^^ Granulocyte macrophage colony stimulating factor ( GM CSF ) , Interleukin 3 ( IL 3 ) , and Steel Factor ( SF ) induce proliferation of hematopoietic cells through binding to specific , high affinity , cell surface receptors . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In order to study its mechanism of action and to explore the molecular basis for its synergistic activity we have examined the proteins that become tyrosine phosphorylated in response to SF , IL 3 , and GM CSF . ^^^ We report herein that SF , but not IL 3 or GM CSF , dramatically stimulates the tyrosine phosphorylation of the product of the recently discovered proto oncogene , vav , in two SF responsive human cell lines , M07E and TF 1 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In the present study , we have addressed the mechanism of synergistic growth activation , at the cellular level , by SF and GM CSF using the blast cells of acute myeloblastic leukemia ( AML blasts ) . ^^^ Analysis of cultures performed in the presence of SF and GM CSF at different cell concentrations , ranging from 1 , 000 to 20 , 000 cells , suggested a single limiting element , i . e . , the blast clonogenic cell , while 2 or more limiting elements were found in cultures stimulated with GM CSF alone , suggesting interacting cell populations . ^^^ In all samples tested , SF appears to increase the responsiveness of AML blasts to GM CSF , as demonstrated by a 3 fold decrease of GM CSF half efficient concentration on addition of SF to the cultures . ^^^ Exposure of AML blasts to SF did not affect GM CSF receptor expression , suggesting that this increase in GM CSF responsiveness is likely to occur at the postreceptor level . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Combinations of two factors that included SF plus IL 6 , IL 11 , or granulocyte colony stimulating factor were all effective in the primary culture in the maintenance of the B lymphoid potential . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
However , in SF conditions the sequential analysis of these rGFs , alone or in combination , clearly showed a stimulating activity of interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) and erythropoietin ( EPO ) on granulopoiesis and erythropoiesis . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Synovial fluids ( SF ) from patients with osteoarthritis ( OA ) and rheumatoid arthritis ( RA ) and various other arthritides were examined for the presence of colony stimulating factors ( CSF ) . ^^^ CSF was found in 7 of 13 ( 54 % ) SF from OA patients and in 8 of 12 ( 67 % ) SF from RA patients . ^^^ While CSF was found in both inflammatory and noninflammatory effusions , significantly greater numbers of colonies were stimulated by RA SF than by OA SF and in general greater numbers of colonies correlated with higher SF leukocyte counts . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We describe a high performance liquid chromatographic method , using electrochemical detection , for the determination of pirprofen in cerebrospinal fluid ( CSF ) , plasma , and synovial fluid ( SF ) . ^^^ The detection limit was 0 . 1 ng / mL for CSF , and 1 ng / mL for plasma and SF , with a recovery greater than 96 % and intraday coefficient of variation less than 5 % in all cases . ^^^ The main advantages of this method include high specificity and sensitivity which allow the analysis of CSF and the use of small volumes of plasma and SF . ^^^ The application of the method for the analysis of plasma and SF samples and the kinetic profile in CSF are shown . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
A liquid culture technique was used to study 23 synovial fluids ( SF ) ( 21 from inflammatory joint diseases and 2 noninflammatory SF ) and supernatants of two cultured rheumatoid arthritis ( RA ) synovial tissues for colony stimulating factor ( CSF ) . ^^^ CSF activity was observed in inflammatory SF , with tritiated thymidine uptake of 3 , 501 + / 1 , 140 cpm in the presence of RA samples ( n = 15 ) compared to 1 , 985 + / 628 for non RA inflammatory SF ( n = 7 ) ( P less than 0 . 05 ) and 583 + / 525 for medium ( n = 6 ) ( P less than 0 . 01 ) . ^^^ Two RA SF were fractionated by Sephadex G 100 column chromatography ; low levels of CSF activity were detected in fractions corresponding to Mr of 70 100 kD , but the major CSF activity was found in the 20 24 kD fractions . ^^^ A polyclonal rabbit anti GM CSF antibody eliminated the stimulating activity from both rGM CSF and RA SF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
A new growth factor ( synergistic factor , SF ) has recently been described , which , in combination with a macrophage CSF source , is able to stimulate the proliferation of primitive high proliferative potential macrophage progenitor cells ( HPP CFC ) in mouse bone marrow in culture . ^^^ It has been found that the addition of supraoptimal amounts of the crude CSF sources , extracts of pregnant mouse uterus and embryo , media conditioned by L cells or the mouse mammary tumor cell line ( EMT 6 ) , stimulated the proliferation of HPP CFC in the absence of any added SF . ^^^ This conclusion is supported by the results obtained from Sephacryl S 200 chromatography of these three CSF sources , which indicate that in all three cases fractions with apparent molecular weight ( MW ) greater than 68 , 000 contained the major portion of the CSF 1 activity , whereas fractions with MW less than 68 , 000 contained the SF like activity , together with minor amounts of CSF 1 activity . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The human factor dependent cell line MO 7 has been used as a model to study the interaction of SF with other growth factors such as GM CSF , because both factors support the proliferation of this cell line and are synergistic in combination . ^^^ In an attempt to further explore the biological and biochemical mechanisms of the synergy between SF and GM CSF , we examined the effects of these growth factors on the regulation of nuclear proto oncogenes , cell cycle control genes , and G 1 > S transition of MO 7 cells . ^^^ Individually , GM CSF was a much more potent growth factor for MO 7 cells than SF , particularly under serum free conditions . ^^^ Only GM CSF , but not SF , was able to stimulate G 1 > S transition of MO 7 cells after factor deprivation for 24 h . ^^^ Northern blot analyses showed also differential effects of GM CSF and SF on the expression of some nuclear proto oncogenes and G 1 cyclins . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
OBJECTIVE To assess the influence of inflammatory synovial fluid ( SF ) on apoptosis of joint and blood neutrophils with particular reference to levels of colony stimulating factors ( CSF ) contained therein . ^^^ Separated neutrophils were cultured in vitro and exposed to : varying concentrations of SF in which CSF levels were measured , recombinant G CSF and GM CSF , and hyaluronic acid control solutions . ^^^ Numbers of apoptotic neutrophils and CSF levels were also measured in fresh SF samples . ^^^ CONCLUSION These data suggest that SF contains a factor or factors capable of directly or indirectly promoting neutrophil apoptosis and normally powerful enough to overcome the apoptosis inhibiting effects of cytokines such as GM CSF at concentrations usually found in inflammatory synovial fluids . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We have probed the pathways activated by SF and GM CSF in suppression of active cell death ( apoptosis ) using two classes of inhibitors : Tyrphostins that are specific inhibitors of protein tyrosine kinase , and amiloride derivatives ( 5 ( N , N ethyl n isopropyl ) amiloride and 5 ( N , N hexamethylene ) amiloride ) that have been designed as specific inhibitors of the Na+ / H+ antiporter . ^^^ Both SF dependent and GM CSF dependent pathways are sensitive to inhibition by Tyrphostins with , nonetheless , a quantitative difference . ^^^ In contrast to the discrepancy in Tyrphostin dose response curves , titration curves for 5 ( N ethyl n isopropyl ) amiloride and 5 ( N , N hexamethylene ) amiloride are comparable in SF or GM CSF stimulated cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Substance P ( SP ) , neurokinin A ( NKA ) , calcitonin gene related peptide ( CGRP ) and neuropeptide Y ( NPY ) like immunoreactivity ( LI ) was studied in rats ' cerebrospinal fluid ( CSF ) , plasma and synovial fluid ( SF ) from both knee joints at 2 and 24 h following an intraperitoneal administration of 0 . 05 ml human recombinant interleukin 1 alpha ( i . p . hrIL 1 alpha ) or saline . ^^^ There was a correlation in CGRP LI content between plasma and CSF following i . p . hrIL 1 alpha but not between plasma and SF or CSF and SF . ^^^ It can be concluded that ( 1 ) i . p . hrIL 1 alpha activates somatosensory afferents thereby increasing SP and CGRP LI content in CSF and plasma and NKA LI in CSF ; 2 ) i . p . hrIL 1 alpha induces a bilateral increase of CGRP LI in SF which is not mediated through systemic circulation and is possibly a part of the general host defensive reaction . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
These results suggest that SF CSF estimations using commercially available assays could provide useful diagnostic clues for clinicians , but careful interpretation is warranted particularly in patients on long term NSAID treatment . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Interleukin 6 ( IL 6 ) , granulocyte colony stimulating factor ( G CSF ) , interleukin 11 ( IL 11 ) , interleukin 4 ( IL 4 ) , interleukin 1 alpha ( IL 1 alpha ) , and tumor necrosis factor alpha ( TNF alpha ) also supported survival of a few progenitors , but much less effectively than either IL 3 or SF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
However , combination of SF with lineage specific factors , such as erythropoietin ( Epo ) or / and granulocyte colony stimulating factor ( G CSF ) was not sufficient for the proliferation of multipotential progenitors ( CFU GEMM ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We tested the hypothesis that the Fanconi anemia mutation results in insufficient production of hematopoietic growth factors by stromal cells by quantifying constitutive and induced production of interleukin 6 ( IL 6 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , granulocyte colony stimulating factor ( G CSF ) , macrophage colony stimulating factor ( M CSF ) , and steel factor ( SF ) by untransformed fibroblasts from eight patients with FA from five different families . ^^^ While no abnormalities were noted in SF or M CSF production , we noted substantial variability in IL 6 , GM CSF , and G CSF responses of cells obtained from different FA patients . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Although the combination of SF , IL 7 , and Ep was sufficient to support formation of lymphomyeloid colonies , addition of IL 11 , granulocyte colony stimulating factor or IL 12 to the combination of SF , IL 7 , and Ep increased the number of lymphomyeloid colonies . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We compared the ability of SF and GR cells to sustain in vitro proliferation of the FDC P 1 myeloid cell line , dependent upon IL 3 or GM CSF . 3 . ^^^ The same activity could be restored to NaCl treated GR cells , but not to SF , by incubation with recombinant murine GM CSF . 4 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
METHODS We measured IL 6 , IL 1 beta , tumour necrosis factor alpha ( TNF alpha ) , granulocyte macrophage colony stimulating factor , IL 8 , and polymorphonuclear leucocyte ( PMNL ) chemotaxis and degranulation in SF from patients with RA ( n = 30 ) in the early phase of the disease . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Cerebrospinal fluid ( CSF ) and synovial fluid ( SF ) were obtained from patients during spinal puncture and athroscopy , respectively . ^^^ Only CSF and SF of patients with proven non pathological conditions were used in this study . ^^^ In non pathological conditions sIL 6R concentrations in CSF are 1 . 6 + / 0 . 4 ng / ml , and in SF 11 . 6 + / 3 . 3 ng / ml , while IL 6 concentrations are below detectable ranges in these fluids . ^^^ CONCLUSIONS : sIL 6R levels are detectable in serum , urine , CSF and SF during non pathological conditions . sIL 6R levels in serum outrange levels in CSF , urine and SF and large interindividual differences in baseline concentrations for sIL 6R exist . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
GF dependent samples had significant proliferative responses to steel factor ( SF ) , GM CSF and IL 3 ; mean Ki67+ cell number increased by 4 . 1 , 3 . 3 , and 5 . 2 fold , respectively . ^^^ SF was additive or synergistic with GM CSF or IL 3 among GF dependent cases ; however , combinations were no more effective than single cytokines among the GF independent group . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Low doses of IL 2 ( 0 . 1 1000 U / ml ) were studied without or in combination with recombinant IL 3 , GM CSF and erythropoietin , in SF long term marrow culture ( LTMC ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The form of the RT / SF function : ( a ) replicated the inverse of the contrast sensitivity function ( CSF ) at near threshold contrast levels ; ( b ) behaved differently at higher contrasts , exhibiting two branches at contrast close to 1 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In two LTCs initiated in the blastic stage , we demonstrated the selective effect of three culture media ( serum dependent , serum free ( SF ) supplemented or not with IL 3 and GM CSF ) on the proliferative potential of normal hematopoietic ( CFU GM / BFU E ) and leukemic progenitors ( CFU ALL ) . ^^^ The addition of IL 3 and GM CSF to the SF medium enhanced the growth of CFU GM / BFU E and shortened the purging period . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Analysis of a panel of samples from different patients with acute myeloblastic leukemia ( AML ) and myeloid leukemic cell lines indicated that IL 11 alone was ineffective in supporting myeloid leukemic cell growth but frequently enhanced growth supported by IL 3 , granulocyte macrophage colony stimulating factor ( GM CSF ) , or SF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In addition , both lipopolysaccharide and granulocyte macrophage colony stimulating factor induced modest increases in IL 1ra mRNA by cultured SF PMN . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Addition of Steel factor ( SF ) , interleukin 6 ( IL 6 ) , or granulocyte colony stimulating factor ( G CSF ) to cultures containing interleukin 3 enhanced blast cell colony formation . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We examined the effects of ML , as a single factor or in combinations with early acting factors such as steel factor ( SF ) , interleukin ( IL ) 3 , IL 1 , IL 6 , and granulocyte colony stimulating factor ( G CSF ) , on colony formation from primitive progenitors of mice . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Steel factor ( SF ) , interleukin 3 ( IL 3 ) , IL 4 , IL 6 , IL 11 , and granulocyte colony stimulating factor ( G CSF ) as single agents supported formation of significant numbers of colonies , but flt3 / flk 2 ligand ( FL ) and IL 12 did not . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
B lymphoid potential was not supported , however , in SF + IL 3 or in SF + IL 3 plus any single growth factor ( IL 1 to 12 , granulocyte macrophage colony stimulating factor [ GM CSF ] , G CSF , erythropoietin [ Epo ] , leukemia inhibitory factor [ LIF ] , tumor necrosis factor alpha [ TNF alpha ] , transforming growth factor beta [ TGF beta ] , gamma interferon [ IFN gamma ] , or insulin like growth factor 1 [ IGF 1 ] ) , but was supported in SF + IL 3 + 5 % PWM SCM . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
However , in vitro stimulation of these progenitor cells using IL 3 , GM CSF , erythropoietin and steel factor ( SF ) revealed that , after 48 h in suspension culture , up to 30 % of the CD34+ cells were in S / G2m phase . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
PGE 2 enhanced the SF and IL 6 dependent development of mast cells from CBMC probably by blocking granulocyte macrophage CSF ( GM CSF ) secretion from accessory cells , because 1 ) PGE 2 , or anti GM CSF enhanced the mast cell development induced by SF and IL 6 from CBMC , but not from CD34+ cells ; 2 ) GM CSF inhibited the enhancing effect of IL 6 on the mast cell development from CD34+ cells ; and 3 ) PGE 2 inhibited GM CSF secretion from CBMC . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
When combined with SF , TPO showed stronger stimulation of production of progenitors in suspension culture than other early acting factors , such as IL 6 , IL 11 , and granulocyte colony stimulating factor ( G CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Monocytes from SF and blood of patients with RA produced highly elevated levels of interleukin 1 alpha ( IL 1 alpha ) , IL 6 , tumor necrosis factor alpha ( TNF alpha ) , and granulocyte macrophage colony stimulating factor ( GMCSF ) , the leading mediators of inflammation . ^^^ The cytokine produced by monocytes ( IL alpha , IL 6 , TNF alpha , and GMCSF ) and by CD4+ T cells Th 2 cell responses ( IL 4 and IL 10 ) may exert immunopathologic and immunoregulatory effects in SF and thus mediate some of the clinical manifestations of RA . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
On the other hand , murine M 2 10B4 fibroblasts engineered to produce high levels of both human granulocyte colony stimulating factor ( G CSF ) and interleukin 3 ( IL 3 ; 190 and 4 ng / mL , respectively ) , either alone or mixed 1 : 1 with SI / SI fibroblasts engineered to produce high levels of soluble Steel factor ( SF ) , with or without production of the transmembrane form of SF ( 60 and 4 ng / mL , respectively ) , stimulated the production of up to 20 fold more CFC in LTC of cells from normal human marrow , G CSF mobilized blood or cord blood when compared with parallel cocultures containing HMF . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Using this approach distinct expression patterns have been shown for the receptors for interleukin 3 ( IL 3 ) , IL 6 , granulocyte / macrophage colony stimulating factor ( GM CSF ) and Steel Factor ( SF ) on subsets of CD34+ and CD 34 cells in bone marrow . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
IGFBP 1 in serum , synovial fluid ( SF ) , cerebrospinal fluid ( CSF ) , and amniotic fluid ( AF ) were measured before and after treatment with alkaline phosphatase ( ALP ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
However , optimal yields of colonies containing cells of both Mk and non Mk lineages required the addition of other growth factors , of which a combination of IL 3 , IL 6 , GM CSF and Steel factor ( SF ) + / TPO was the best of those tested . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Previous studies have shown that primitive human hematopoietic cells detectable as long term culture initiating cells ( LTC ICs ) and colony forming cells ( CFCs ) can be amplified when CD 34 ( + ) CD 38 ( ) marrow cells are cultured for 10 days in serum free medium containing flt 3 ligand ( FL ) , Steel factor ( SF ) , interleukin ( IL ) 3 , IL 6 , and granulocyte colony stimulating factor . ^^^ Thus , maximal expansion of LTC ICs ( 60 fold ) was obtained in the presence of 30 times more FL , SF , IL 3 , IL 6 , and granulocyte colony stimulating factor than could concomitantly stimulate the near maximal ( 280 fold ) amplification of CFCs . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Results show that ( 1 ) color CSF ' s are a low pass function of SF , whereas TE curves are a bandpass function of mask SF , and ( 2 ) a minimum of six SF tuned color mechanisms ( one low pass and five bandpass functions of SF , with peak SF ' s of 0 . 13 , 0 . 5 , 2 , 4 , and 8 cycles per degree and bandwidths of 3 . 9 , 4 . 4 , 2 . 9 , 2 . 1 , 1 . 1 , and 1 . 2 octaves ) , similar to oblique masking color mechanisms , are extracted by the multiple mechanisms model . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
To address the possibility that SCL may function upstream of c kit , we sought to modulate endogenous SCL function in the CD 34 ( + ) hemopoietic cell line TF 1 , which requires SF , granulocyte / macrophage colony stimulating factor , or interleukin 3 for survival . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The further observation that SF EC cultures elaborated comparable amounts of granulocyte macrophage ( GM ) CSF as FCS cultured EC , but were relatively impaired in their IL 1alpha and TNF alpha production , supports the role of GM CSF in LC maturation and , less so , in LC survival . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Urine , cerebrospinal fluid ( CSF ) , and SF specimens from 57 patients with Lyme borreliosis and from 58 controls were examined . ^^^ In clinical samples the diagnostic sensitivity of PCR was 85 % with SF samples , 79 % with urine samples , and 91 % with paired SF urine samples from patients with Lyme arthritis and was 79 % with CSF samples , 45 % with urine samples , and 87 % with paired CSF urine specimens from neuroborreliosis patients . ^^^ At least two different primer sets should be used , and whenever possible , urine and CSF or SF should be analyzed in parallel to achieve maximum sensitivity of the test . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Since SF DC have differentiated in vivo in the presence of proinflammatory cytokines , PB DC were cocultured in the presence of IL 10 and either GM CSF , IL 1beta , TNF alpha , IL 6 , or TGF beta . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Constitutive Cdc25A protein levels were enhanced with granulocyte macrophage colony stimulating factor ( GM CSF ) plus steel factor ( SF ) . ^^^ Cdc25A is thought to exert its activity in the nucleus , and nuclear protein levels of Cdc25A were also enhanced with GM CSF and SF . ^^^ GM CSF plus SF promote synergistic growth of Mo7e cells . ^^^ Pretreatment with macrophage inflammatory protein ( MIP 1alpha ) inhibited GM CSF plus SF induced growth and upregulation of Cdc25A protein levels . ^^^ Stimulation with GM CSF and SF also rapidly increased Cdc25A phosphatase activity , an effect suppressed by MIP 1alpha . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
OBJECTIVE : We have investigated the effects of interleukin ( IL ) 10 , IL 4 + granulocyte / macrophage colony stimulating factor ( GM CSF ) and tumour necrosis factor alpha ( TNF alpha ) on the phenotype and antigen presenting capacity of synovial fluid ( SF ) macrophages from patients with rheumatoid arthritis . ^^^ METHODS : The effects of IL 4 , IL 10 , GM CSF and TNF alpha on the expression of surface antigens on SF macrophages were studied using flow cytometry . ^^^ IL 4 + GM CSF , a combination of cytokines known to induce differentiation of dendritic cells , increased the expression of CD 40 , CD 80 and CD 86 , and decreased the expression of CD 14 on SF macrophages . ^^^ Accordingly , IL 4 + GM CSF increased the capacity of SF macrophages to activate T cells in MLR . ^^^ IL 10 inhibited the effects of IL 4 + GM CSF on SF macrophages . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Similarly , high BMP 9 concentrations dramatically inhibited primary colony formation induced by SF+IL 11 / 12 , whereas in the presence of TGF beta 1 neutralizing Ab only 3 ng or more BMP 9 per ml stimulated both the time of colony appearance , the colony size and colony numbers in the presence of IL 3 , M CSF , GM CSF , SF , SF+Flt3 L , SF+IL 3 , SF+IL 11 / 12 or IL 3+SF+IL 11 / 12 . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Normal bone marrow cells or Thy 1 . 1lowSca 1+Lin ( neg ) HSPC were incubated in methylcellulose cultures and stimulated with various combinations of steel factor ( SF ) , interleukin ( IL ) 3 , IL 6 , granulocyte colony stimulating factor ( G CSF ) , and erythropoietin ( EPO ) in the presence of increasing concentrations of Cy A . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
OBJECTIVES : The purpose of this study was to analyse the presence of the granulocyte macrophage colony stimulating factor ( GM CSF ) in human cerebrospinal fluid ( SF ) of patients affected by multiple sclerosis ( MS ) in comparison with non inflammatory neurological diseases . ^^^ RESULTS : We found that GM CSF is constitutively present in human SF ; this presence was confirmed by its stimulating activity of colony forming unit granulocyte macrophage ( CFU GM ) production . ^^^ CONCLUSION : Our data suggest that GM CSF is a constitutive component of human SF , relatively uninfluenced by the different morbid conditions of the nervous system . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We studied O 2 ( o ) production and p47phox phosphorylation in synovial fluid ( SF ) from patients with rheumatoid arthritis ( RA ) and spondylarthropathy ( SpA ) according to TNFalpha , IL 8 and GM CSF levels . ^^^ High levels of TNFalpha and IL 8 ( but not GM CSF ) are detected in patient ' s SF ( compared to circulating blood levels ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
METHODS : Cardiac samples from 15 autopsied subjects ( HD , n=6 ; control , n=9 ) were sequentially extracted with 0 . 9 % NaCl and collagenase to obtain two fractions [ soluble fraction ( SF ) and collagenase soluble fraction ( CSF ) ] . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Culture of AML cells in serum free medium with 100 ng / ml Steel factor ( SF ) , 20 ng / ml interleukin 3 ( IL 3 ) and 20 ng / ml granulocyte colony stimulating factor ( G CSF ) for 16 24 h maintained the number of AML CFC and LTC IC at near input values ( mean % input + / s . d . for CFC and LTC IC were 78 + / 33 and 126 + / 53 , respectively ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Despite the incapacity of SF to sustain FDC P 1 cells , the biologically active GM CSF could be recovered from all the studied stromas by treatment with high salt buffers that release non covalently bound molecules from stroma cells . ^^^ Glycosaminoglycans purified from stromas had distinct effect on the GM CSF mediated proliferation of FDC P 1 cells : those purified from S 17 and GR cells were stimulatory , whereas those obtained from SF cells were slightly stimulatory at low concentration , but inhibitory at the higher ones . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
We found that several HGFs , including granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) , steel factor ( SF ) , and thrombopoietin ( TPO ) induce a rapid increase in reactive oxygen species ( ROS ) in quiescent cells . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Results show the following : ( 1 ) The color CSF is a low pass function of SF with average half height SF of 0 . 7 cpd and cutoff SF of 14 cpd with the use of a color detection criterion . ( 2 ) TvSF curves are broadly bandpass and fall into five groups , peaking at approximately 0 . 13 , 0 . 5 , 2 , 4 , and 8 cpd . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Significantly higher levels of proliferation and expansion of progenitor cells were observed in the presence of SF , IL 6 , GM CSF , and G CSF with preferential stimulation of myelopoiesis . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Concentrations of the cytokines known to induce CD 16 ( including transforming growth factor beta 1 [ TGFbeta 1 ] , macrophage colony stimulating factor [ M CSF ] , and interleukin 10 [ IL 10 ] ) and concentrations of the soluble form of CD 14 ( sCD 14 ) in plasma and synovial fluid ( SF ) samples were measured by enzyme linked immunosorbent assay . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Sera , synovial ( SF ) and cerebro spinal fluids ( CSF ) were obtained from 90 patients with clinical symptoms of Lyme borreliosis . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
All 4 AML samples showed evidence of autocrine production of 2 or more of SF , FL , IL 3 , and granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
In the current study , AML cell engraftment in standard NOD / SCID mice was compared to that obtained with NOD / SCID mice transgenic for the human growth factor genes Steel factor ( SF ) , interleukin 3 ( IL 3 ) and granulocyte macrophage colony stimulating factor ( GM CSF ) ( N / S S / GM / 3 ) as well as beta 2 microglobulin null NOD / SCID ( N / S beta 2m ( / ) ) mice . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Here , we show that nonobese diabetic severe combined immunodeficiency mice genetically engineered to produce ng / ml serum levels of human interleukin 3 ( IL 3 ) , granulocyte / macrophage stimulating factor ( GM CSF ) and Steel factor ( SF ) display a complex phenotype when transplanted with primitive human bone marrow ( BM ) or fetal liver cells . ^^^ Our findings suggest that long term exposure of primitive human hematopoietic cells to elevated levels of human IL 3 , GM CSF and SF in vivo may deleteriously affect the stem cell compartment , while expanding terminal myelopoiesis . . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
Monocytes from healthy donors were differentiated in vitro into DC with granulocyte macrophage colony stimulating factor ( GM CSF ) and interleukin ( IL ) 4 , in the absence or presence of soluble factors ( SF ) from SCC or AD cell lines . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
The messenger RNA expressions of LARC ( liver and activation regulated chemokine ) , GMCSF ( granulocyte macrophage colony stimulating factor ) , epiregulin , ICAM 1 ( intercellular adhesion molecule 1 ) , and TGFA ( transforming growth factor alpha ) were more strongly up regulated by IL 1alpha and / or IL 1beta in MECF than in SF , suggesting that these fibroblasts derived from different tissues retained their typical gene expression profiles . ^^^
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P21583 and P04141	Pubmed	SVM Score :0.0
NA