| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| Synaptotagmin 4 ( Syt 4 ) is an inducible member of a multi gene family of synaptic vesicle proteins that participate in Ca2+ dependent and Ca2+ independent interactions during membrane trafficking . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| Synaptotagmin 4 ( Syt 4 ) is an immediate early gene induced by depolarization in rat PC 12 cells and in rat hippocampus . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| Variations of synaptotagmin 1 , synaptotagmin 4 , and synaptophysin mRNA levels in rat hippocampus during the estrous cycle . ^^^ To investigate the molecular mechanisms involved in synaptic remodeling during the estrous cycle , we analyzed the expression of three presynaptic markers , synaptotagmin 1 , synaptotagmin 4 , and synaptophysin , in the female adult rat brain by in situ hybridization . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| In 6 day old rat cerebellum and cultured hippocampal neurons the subcellular distribution of synaptotagmin 4 was clearly different from that of synaptotagmin 1 . ^^^ Synaptotagmin 4 displayed a punctate non polarized distribution on neuronal extensions , whereas synaptotagmin 1 staining was essentially synaptic . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| Rat synaptotagmin 4 ( SYT 4 ) is a depolarization inducible synaptic vesicle protein . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| The SNARE proteins , VAMP 4 and synaptotagmin 4 ( Syt 4 ) , enter ISGs initially but are sorted away during maturation . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| Overexpression of synaptotagmin 1 prolonged the time from fusion pore opening to dilation , whereas synaptotagmin 4 shortened this time . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| We isolated the rat synaptotagmin 4 ( Syt 4 ) cDNA in a screen for sequences that are specifically induced in neuronal cells . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| Similarly , we found that synaptotagmin 4 could substitute for synaptotagmin 1 . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| Synaptotagmin 4 ( Syt 4 ) expression is regulated by neuronal development and by depolarization in the brain and in neuronal cell cultures . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| Synaptotagmin 4 ( Syt 4 ) is a fourth member of the Syt family and has been shown to regulate some forms of memory and learning by analysis of Syt 4 null mutant mice ( Ferguson , G . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| Synaptotagmin 4 ( Syt 4 ) is a secretory vesicle protein that is broadly expressed in brain and may function as a presynaptic regulator of synaptic release . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| Synaptotagmin 4 ( Syt 4 ) is an activity inducible , secretory vesicle protein that is thought to function as an inhibitor of neurotransmitter release ( Littleton et al . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| Synaptotagmin 4 ( Syt 4 ) was originally described as an immediate early gene product induced by forskolin or membrane depolarization in PC 12 cells ; however , nothing is known about the subcellular localization and transport of the newly translated Syt 4 protein in PC 12 cells . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| An isoform of the synaptotagmin family , synaptotagmin 4 ( Syt 4 ) , serves as a postsynaptic Ca2+ sensor to release retrograde signals that stimulate enhanced presynaptic function through activation of the cyclic adenosine monophosphate ( cAMP ) cAMP dependent protein kinase pathway . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| Synaptotagmin 4 ( Syt 4 ) is a brain specific isoform of the synaptotagmin family , the levels of which are strongly elevated after seizure activity . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| We present evidence that the ISG localized synaptotagmin 4 ( Syt 4 ) is involved in ISG maturation . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| However , little is known about the function of SytIV or the functional relationship between SytIV and SytI , because SytIV has yet to be localized . ^^^ These SytIV signals were not colocalized well with SytI signals . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| Two synaptotagmin genes , Syt 1 and Syt 4 , are differentially regulated in adult brain and during postnatal development following kainic acid induced seizures . ^^^ In the present study , we examined the time course of the seizure induced changes in the expression of Syt 4 and Syt 1 , both in adult animals and during the postnatal period . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| Syt 4 expression is induced in the rat hippocampus after systemic kainic acid treatment . ^^^ To examine the functional role of this protein in vivo , we derived Syt 4 null [ Syt 4 ( / ) ] mutant mice . ^^^ Studies with the rotorod revealed that the Syt 4 mutants have impaired motor coordination , a result consistent with constitutive Syt 4 expression in the cerebellum . ^^^ Because Syt 4 is thought to modulate synaptic function , we also have examined Syt 4 mutant mice in learning and memory tests . ^^^ Our studies show that the Syt 4 mutation disrupts contextual fear conditioning , a learning task sensitive to hippocampal and amygdala lesions . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| Group C synaptotagmins are characterized by weak Ca ( 2+ ) dependent ( Syts 9 ) or no homo oligomerization activity ( Syt 4 ) . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| Here we investigate whether dopaminergic regulation of syt 1 and syt 4 expression could play a role in the hypersensitive striatum of rats with unilateral lesions of dopaminergic nigrostriatal neurons with 6 hydroxydopamine . ^^^ We show that chronic dopaminergic denervation resulted in a small down regulation of striatal syt 1 mRNA , whereas acute treatment with SKF 82958 , a dopamine D 1 receptor agonist , induced a massive syt 4 mRNA upregulation in the striatum on the lesioned side . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| Immunocytochemical analysis revealed that of the candidate Ca ( 2+ ) sensors in LDCV exocytosis , syt 3 was not expressed in primary beta cells , whereas syt 4 was only found adjacent to the TGN . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| Syt 4 is mainly localized at the Golgi , while Syt 1 , a possible Ca ( 2+ ) sensor for secretory vesicles , is localized at dense core vesicles and synaptic like microvesicles in PC 12 cells . ^^^ In this study , we sought to identify the region responsible for the Golgi localization of Syt 4 by immunocytochemical and biochemical analyses as a means of defining the distinct subcellular localization of the synaptotagmin family . ^^^ We found that the unique C terminus of the spacer domain ( amino acid residues 73 144 ) between the transmembrane domain and the C2A domain is essential for the Golgi localization of Syt 4 . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| We recently showed that deletion of the short C terminus ( 17 amino acids ) of Syt 4 prevented the Golgi localization of Syt 4 proteins in PC 12 cells and induced granular structures of various sizes in the cell body by an unknown mechanism ( Fukuda , M . , Ibata , K . , and Mikoshiba , K . ( 2001 ) J . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| The activation of C2B by C2A was also displayed by the C 2 domains of syt 3 but not the C 2 domains of syt 4 . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| Endogenous Syt 4 , induced by forskolin treatment , had a similar effect . ^^^ Full fusion was inhibited by mutation of a Ca2+ ligand in the C2A domain of Syt 1 ; kiss and run was inhibited by mutation of a homologous Ca2+ ligand in the C2B domain of Syt 4 . ^^^ The Ca2+ sensitivity for full fusion was 5 fold higher with Syt 1 than Syt 4 , but for kiss and run the Ca2+ sensitivities differed by a factor of only two . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| In the present study , it has been shown by immunoaffinity purification and immunocytochemistry that Syts 1 and 9 , but not Syt 4 , are present on the same secretory vesicles in PC 12 cells . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.60743471 |
| Synaptotagmin 4 forms hetero oligomers with synaptotagmin 1 , resulting in synaptotagmin clusters that can not effectively penetrate lipid bilayers and are less efficient at coupling Ca2+ to secretion in vivo : upregulation of synaptotagmin 4 , but not synaptotagmin 1 , decreases evoked neurotransmission . 0.60743471^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.54902636 |
| Conversely , the C2B domain of Syt 4 contains calcium binding properties , which permit homo oligomerization as well as hetero oligomerization with Syt 1 . 0.54902636^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| Whereas the functions of the other Syts are unclear , Syt 4 is of particular interest because it is rapidly up regulated after chronic depolarization or seizures , and because null mutations exhibit deficits in fine motor coordination and hippocampus dependent memory . ^^^ Screening Syts 1 13 , which are enriched in brain , we find that Syt 4 is located in processes of astroglia in situ . ^^^ Reduction of Syt 4 in astrocytes by RNA interference decreases Ca ( 2+ ) dependent glutamate release , a gliotransmission pathway that regulates synaptic transmission . ^^^ Mutants of the C2B domain , the only putative Ca ( 2+ ) binding domain in Syt 4 , act in a dominant negative fashion over Ca ( 2+ ) regulated glial glutamate release , but not gliotransmission induced by changes in osmolarity . ^^^ Because we find that Syt 4 is expressed predominantly by astrocytes and is not in the presynaptic terminals of the hippocampus , and because Syt 4 knockout mice exhibit hippocampal based memory deficits , our data raise the intriguing possibility that Syt 4 mediated gliotransmission contributes to hippocampal based memory . . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| Syt 7 GFP forms a complex with endogenous Syts 1 and 9 , but not with Syt 4 , and it colocalize well with Syts 1 and 9 in the cellular processes ( where dense core vesicles are accumulated ) in the PC 12 cell line . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| Of the five evolutionarily conserved isoforms in Drosophila , only two , syt 1 and syt 4 , localize to most , if not all , synapses . ^^^ This suggests an intriguing possibility that syt 4 may mediate a postsynaptic vesicle trafficking pathway , providing a molecular basis for an evolutionarily conserved bidirectional vesicular trafficking communication system at synapses . . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| On the 6 hydroxydopamine ( 6 OHDA ) lesioned side we observed a nearly total loss of tyrosine hydroxylase ( TH ) , synaptotagmin 1 , Syt 4 , Syt 7 and Syt 11 mRNA levels in the substantia nigra compacta ( SNc ) . ^^^ By contrast , these two drugs induced an increase of Syt 4 and Syt 7 mRNA levels . ^^^ A time course study revealed the highest levels of Syt 4 and 7 mRNAs to occur at two hours and 12 hours after the treatment with SKF 82958 , respectively . ^^^ D 1 antagonist ( + / ) 7 chloro 8 hydroxy 3 methyl 1 phenyl 2 , 3 , 4 , 5 tetrahydro 1H 3 benzazepine hydrochloride ( SCH 23390 ) but not D 2 antagonist haloperidol prevented the L DOPA driven increase of Syt 4 and 7 mRNAs . ^^^ The L DOPA treatment does not reverse the changes in Syt 2 and Syt 10 gene expression , but recruits additional , D 1 receptor mediated changes in Syt 4 and Syt 7 gene expression . ^^^ |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9H2B2 and P21579 |
Pubmed |
SVM Score :0.0 |
| NA |
|