| Interacting proteins: Q9BQG1 and P21579 |
Pubmed |
SVM Score :0.0 |
| The high affinity calcium sensor synaptotagmin 3 ( Syt 3 ) was localized to the synaptic layers of both goldfish and rodent retinas ; however , while Syt 3 was associated with PKC labeled bipolar cell terminals in the goldfish retina , it did not co localize with PKC in the mouse retina . ^^^ |
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| Interacting proteins: Q9BQG1 and P21579 |
Pubmed |
SVM Score :0.0 |
| The same C 2 domains also bind syntaxin as a function of Ca2+ but the Ca ( 2+ ) concentration dependence of Syt 1 , 2 and 5 ( > 200 microM ) differs from that of Syt 3 and 7 ( < 10 microM ) . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^ |
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| Interacting proteins: Q9BQG1 and P21579 |
Pubmed |
SVM Score :0.0 |
| In the most highly conserved C 2 domain , the mammalian synaptotagmins , SYT 1 and SYT 2 , share 88 % sequence identity , whereas SYT 3 has only approximately 45 % identity to either . ^^^ Furthermore , in PC 12 cells , Syt 3 is coexpressed with Syt 1 and is more abundant than the latter . ^^^ |
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| Interacting proteins: Q9BQG1 and P21579 |
Pubmed |
SVM Score :0.0 |
| The synaptotagmin 3 gene is expressed in most neurons , but transcripts are much less abundant than the products of the synaptotagmin 1 and 2 genes . ^^^ |
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| Interacting proteins: Q9BQG1 and P21579 |
Pubmed |
SVM Score :0.0 |
| Rat synaptotagmin 3 is a protein of 588 amino acids having 40 . 5 , 38 . 3 , and 64 . 0 % identity with rat synaptotagmin 1 , rat synaptotagmin 2 , and o p 65 C , a third synaptotagmin isoform of marine ray Discopyge ommata , respectively . ^^^ |
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| Interacting proteins: Q9BQG1 and P21579 |
Pubmed |
SVM Score :0.0 |
| Synaptotagmin 1 and 2 have similar patterns and rise to maximum ( adult ) levels around P 14 , whereas synaptotagmin 3 and 4 reach their maximum levels considerably earlier , at P 1 . ^^^ While the developmental expression patterns of synaptotagmin 1 and 2 parallels the temporal development of synaptogenesis in the nervous system , the early maximal expression of synaptotagmin 3 and 4 suggests that these isoforms may have other functions during early postnatal development . . ^^^ |
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| Interacting proteins: Q9BQG1 and P21579 |
Pubmed |
SVM Score :0.0 |
| In these investigations we found that the same treatment induced accumulation over 2 fold of other proteins in the secretory pathway , including synaptosomal associated protein of 25 kDa ( SNAP 25 ) , synaptotagmin 3 , synaptobrevin , synaptophysin , and cyclophilin B , and did not affect accumulation of others , including synaptotagmin 1 , calnexin , and glucose regulated protein 94 . ^^^ |
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| Interacting proteins: Q9BQG1 and P21579 |
Pubmed |
SVM Score :0.0 |
| In this study , we show that synaptotagmin 3 ( Syt 3 ) , a member of the Syt family of proteins , is required for the formation and delivery of cargo to the perinuclear endocytic recycling compartment ( ERC ) . ^^^ |
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| Interacting proteins: Q9BQG1 and P21579 |
Pubmed |
SVM Score :0.0 |
| Chem . 273 , 12267 12273 ) forms beta mercaptoethanol sensitive homodimers and identify three evolutionarily conserved cysteine residues at the N terminus ( N terminal cysteine motif , at amino acids 10 , 21 , and 33 of mouse Syt 3 ) that are not conserved in other isoforms . ^^^ Site directed mutagenesis of these cysteine residues and co immunoprecipitation experiments clearly indicate that the first cysteine residue is essential for the stable homodimer formation of Syt 3 , 5 , or 6 , and heterodimer formation between Syts 3 , 5 , 6 , and 10 . ^^^ We also show that native Syt 3 from mouse brain forms a beta mercaptoethanol sensitive homodimer . ^^^ Our results suggest that the cysteine based heterodimerization between Syt 3 and Syt 5 , 6 , or 10 , which have different biochemical properties , may modulate the proposed function of Syt 3 as a putative high affinity Ca ( 2+ ) sensor for neurotransmitter release . . ^^^ |
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| Interacting proteins: Q9BQG1 and P21579 |
Pubmed |
SVM Score :0.0 |
| The syt 2 gene structures are quite similar to the mouse syt 8 and human syt 9 genes , but not Caenorhabditis elegans syt 1 , human syt 7 , or the mouse syt 3 genes , indicating that the exon intron patterns of the functional domain of synaptotagmins ( especially the C2A domain ) have not been as well conserved during evolution as among isoforms . ^^^ |
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| Interacting proteins: Q9BQG1 and P21579 |
Pubmed |
SVM Score :0.0 |
| Syt 3 and 7 proteins were identified in rat islets and betaTC 3 and RINm5F beta cells by immunoblotting . ^^^ Confocal microscopy showed that Syt 3 and 7 co localized with insulin containing secretory granules . ^^^ Two fold overexpression of Syt 3 in RINm5F beta cell ( Syt 3 cell ) was achieved by stable transfection , which conferred greater Ca ( 2+ ) sensitivity for exocytosis , and resulted in increased insulin secretion . ^^^ Glyceraldehyde + carbachol induced insulin secretion in Syt 3 cells was 2 . 5 fold higher than control empty vector cells , whereas potassium induced secretion was 6 fold higher . ^^^ In permeabilized Syt 3 cells , Ca ( 2+ ) induced and mastoparan induced insulin secretion was also increased . ^^^ |
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| Interacting proteins: Q9BQG1 and P21579 |
Pubmed |
SVM Score :0.0 |
| Rat basophilic leukemia cells ( RBL 2H3 ) , a tumor analogue of mucosal mast cells , express the Syt homologues Syt 2 , Syt 3 and Syt 5 Expression of Syt 1 , the neuronal Ca2+ sensor , in the RBL cells , resulted in its targeting to secretory granules and in prominent potentiation and acceleration of Ca2+ dependent exocytosis . ^^^ |
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| Interacting proteins: Q9BQG1 and P21579 |
Pubmed |
SVM Score :0.0 |
| Immunocytochemical analysis revealed that of the candidate Ca ( 2+ ) sensors in LDCV exocytosis , syt 3 was not expressed in primary beta cells , whereas syt 4 was only found adjacent to the TGN . ^^^ In streptolysin O permeabilized primary beta cells the introduction of recombinant peptides ( 100 nM ) corresponding to the C ( 2 ) domains of syt 5 , 7 and 8 , but not of syt 3 , 4 or 6 , inhibited Ca ( 2+ ) evoked insulin exocytosis by 30 % without altering GTP gamma S induced release . ^^^ Our observations demonstrate that syt 3 and 4 are not involved in the exocytosis of LDCVs from primary beta cells whereas 5 , 7 and 8 may mediate Ca ( 2+ ) regulation of exocytosis . . ^^^ |
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| Interacting proteins: Q9BQG1 and P21579 |
Pubmed |
SVM Score :0.0 |
| The activation of C2B by C2A was also displayed by the C 2 domains of syt 3 but not the C 2 domains of syt 4 . ^^^ |
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| Interacting proteins: Q9BQG1 and P21579 |
Pubmed |
SVM Score :0.0 |
| Rat basophilic leukemia cells ( RBL 2H3 ) , a tumor analogue of mucosal mast cells ( MMC ) , express at least four distinct Syt homologues , including Syt 2 , Syt 3 , Syt 5 and Syt 9 . ^^^ |
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| Interacting proteins: Q9BQG1 and P21579 |
Pubmed |
SVM Score :0.0 |
| Silencing of Syt 9 by RNA interference adenovirus in islet cells had no effect on the expression of Syt 5 , Syt 7 and Syt 3 isoforms . ^^^ |
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| Interacting proteins: Q9BQG1 and P21579 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9BQG1 and P21579 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9BQG1 and P21579 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9BQG1 and P21579 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9BQG1 and P21579 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9BQG1 and P21579 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9BQG1 and P21579 |
Pubmed |
SVM Score :0.0 |
| NA |
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