| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.68936483 |
| In the present study , 1 discovered that dithreonine residues ( Thr 15 and Thr 16 ) at the intravesicular domain of mouse Syt 1 are post translationally modified by a complex form of O linked sugar ( i . e . the addition of sialic acids ) in PC 12 cells and that the O glycosylation of Syt 1 in COS 7 cells depends on the coexpression of vesicle associated membrane protein 2 ( VAMP 2 ) / synaptobrevin . 1 also showed that a transmembrane domain of Syt 1 directly interacts with isolated VAMP 2 , but not VAMP 2 , in the heterotrimeric SNARE ( SNAP receptor ) complex ( vesicle SNARE , VAMP 2 , and two target SNAREs , syntaxin IA and SNAP 25 ) . 0.68936483^^^ |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| In situ hybridization using isoform specific oligonucleotide probes to different exocytotic protein messenger RNAs ( mRNAs ) showed strong hybridization signal for synaptotagmin 1 , cysteine string protein ( CSP ) , VAMP 2 ( vesicle associated membrane protein ) , cellubrevin , munc 18 ( mammalian homologue of unc 18 ) , SNAP 25a ( synaptosomal associated protein of 25 kDa ) , syntaxin 1A , syntaxin 4 , syntaxin 5 , and alpha SNAP ( soluble NSF attachment protein ) in the anterior and intermediate , but not in the posterior lobe of the pituitary . ^^^ |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| Synaptotagmin 1 , vamp 2 , and synapsin 1 proteins are synthesized at nearly constant rates as the neurons develop . ^^^ Despite the nearly constant rates of synthesis of synaptotagmin 1 , vamp 2 , and synapsin 1 , we show that the number of SVs in these developing neurons increases , and that SV proteins are more efficiently targeted to SVs at later times in culture . ^^^ |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| By double immunostaining with specific antibodies against synaptophysin , synaptotagmin 1 , VAMP 1 and VAMP 2 , we identified different relative levels of these integral membrane proteins of synaptic vesicles in comparison to boutons of the same rat cortical neuron . ^^^ |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| The effect of SypI on VAMP 2 sorting was dose dependent , being reversed by increasing VAMP 2 expression levels , and highly specific , because the sorting of the SV proteins VAMP 1 and SytI was not affected by SypI . ^^^ |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| In neurons the core SNARE ( soluble N ethylmaleimide sensitive factor attachment protein receptor ) complex , composed of syntaxin ( Stx ) , SNAP 25 , and VAMP 2 , mediates vesicle fusion , whereas calcium regulation is thought to be accomplished by the synaptotagmin ( Syt ) family , some of which exhibit calcium dependent binding to syntaxin and SNAP 25 . ^^^ |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| Using cell fractionation and immunocytochemical methods , it was revealed that VAMP 1 , VAMP 2 and synaptotagmin 1 ( the putative Ca ( 2+ ) sensor ) localized to subpopulations of ANP containing secretory granules in atrial myocytes . ^^^ |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| In this issue of Neuron , Fernandez Alfonso et al . present data indicating that at least two synaptic vesicle proteins , synaptotagmin 1 and VAMP 2 , are present in a large pool on the synaptic and axonal plasma membrane and can interchange with recently exocytosed proteins . ^^^ |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| Here we present several lines of evidence showing that the Syt 4 protein in PC 12 cells is normally localized in the Golgi or immature vesicles at the cell periphery and is sorted to fusion competent mature dense core vesicles in response to short nerve growth factor ( NGF ) stimulation . ( 1 ) In undifferentiated PC 12 cells , Syt 4 protein is mainly localized in the Golgi and small amounts are also present at the cell periphery , but according to the results of an immunocytochemical analysis , they do not colocalize with conventional secretory vesicle markers ( Syt 1 , Syt 9 , Rab3A , Rab27A , vesicle associated membrane protein 2 , and synaptophysin ) at all . ^^^ |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| Synaptotagmin 1 , synaptobrevin 2 , and syntaxin 1 are coexpressed in rat and gerbil pinealocytes . ^^^ Antibodies against synaptotagmin 1 , synaptobrevin 2 , and syntaxin 1 label pinealocytes ( identified with an antiserum directed against synaptophysin 1 ) in pineal glands of both species , the coexpression of the latter proteins being demonstrable at the single cell level . ^^^ Immunogold staining has been performed in the gerbil pineal and reveals that synaptobrevin 2 and synaptotagmin 1 can be localized to the synaptic like microvesicles that are concentrated in pinealocyte process terminals . ^^^ |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| We proved its coexpression with other synaptic membrane proteins ( synaptophysin , synaptotagmin 1 , synaptobrevin 2 , and syntaxin 1 ) at the single cell level . ^^^ |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| Using indirect immunofluorescence combined with confocal laser scanning microscopy , it was observed that antisera against integral synaptic vesicle membrane proteins ( SV 2 , synaptotagmin 1 , synaptobrevin 2 and synaptophysin ) induced strong immunoreactivities in these cells , but anti synaptobrevin 1 caused only a faint fluorescence . ^^^ |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| Using immunofluorescence microscopy , we found that cultured pinealocytes readily expressed all synaptic membrane proteins investigated , i . e . , synaptophysin , synaptotagmin 1 , synaptobrevin 2 , syntaxin 1 and SNAP 25 . ^^^ |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| Chronic haloperidol treatment caused significant decreases in the levels of synaptotagmin 1 and 4 , synaptobrevin 2 , syntaxin 1A and Rab 3A mRNAs in the nucleus accumbens but not in the prefrontal cortex medial field , striatum , substantia nigra and ventral tegmental area . ^^^ |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| Other known SNARE and SNARE associated proteins such as vesicle associated membrane protein ( VAMP ) / synaptobrevin 2 , SNAP 25 , synaptophysin , or synaptotagmin 1 could not be immunochemically detected in platelet membrane preparations . ^^^ |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| No differences were observed in the amount of synaptosomal associated protein of 25 kDa , synaptobrevin 2 , syntaxin 1 , synaptotagmin 1 and Rab3a in total brain homogenates from treated rats after E 2 administration . ^^^ |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| Here we demonstrate the astrocytic expression and organellar association of a large variety of synaptic proteins ( synaptobrevin 2 , synaptotagmin 1 , synaptophysin , rab3a , synapsin 1 , SNAP 25 , and syntaxin 1 ) and also of the ubiquitous cellubrevin . ^^^ Rab3a and synaptobrevin 2 were retained at a high level and synaptotagmin 1 , synapsin 1 , and syntaxin 1 at a lower level until 20 DIV . ^^^ |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| Labeling of synaptic vesicles prepared from adult brain with [ 3H ] palmitate revealed synaptobrevin 2 besides synaptotagmin 1 as major palmitoylated proteins . [ 3H ] Palmitoylation of synaptobrevin 2 was resistant to chloroform / methanol extraction , but sensitive to reducing agents indicating a covalent fatty acid bond to cysteine residues . ^^^ |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| METHODS : Levels of mRNAs encoding four synaptic vesicle proteins ( synaptotagmin 1 [ p 65 ] , rab3a , synaptobrevin 1 , and synaptobrevin 2 ) and two synaptic plasma membrane proteins ( syntaxin 1A and SNAP 25 ) were measured postmortem in the left superior temporal gyrus from elderly ( 58 95 years ) schizophrenic patients ( n = 14 ) and age matched control subjects ( n = 9 ) . ^^^ |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| Using indirect immunofluorescence microscopy , we analyzed the spatial and temporal distribution of 11 important membrane and membrane associated synaptic proteins ( syntaxin 1 / 3 , SNAP 25 , synaptobrevin 2 , synaptogyrin , synaptotagmin 1 , SV2A , SV2B , Rab3A , clathrin light chains , CSP and neuroligin 1 ) during synaptogenesis . ^^^ |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| Imaged simultaneously in clonal insulin secreting cells by dual color total internal reflection fluorescence microscopy , monomeric red fluorescent protein ( mRFP ) tagged neuropeptide Y and green fluorescent protein ( GFP ) tagged synaptotagmin 1 or synaptobrevin 2 rapidly diffused from sites of exocytosis , whereas the vesicle membrane protein phogrin and tissue plasminogen activator ( tPA ) were retained , consistent with fusion pore closure . ^^^ |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| Interestingly , the properties of basal neurotransmission are normal in the Mecp 2 null mice , consistent with our observations that the levels of expression of synaptic and cytoskeletal proteins , including glutamate receptor subunits GluR 1 and GluR 2 , PSD 95 , synaptophysin 1 , synaptobrevin 2 , synaptotagmin 1 , MAP 2 , betaIII tubulin and NF 200 , are not significantly altered . ^^^ |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| When green fluorescent protein labeled versions of the vesicle proteins synaptobrevin 2 and synaptotagmin 1 are overexpressed in rat hippocampal neurons , up to 30 % are found on axonal membranes under resting conditions . ^^^ |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63027 and P21579 |
Pubmed |
SVM Score :0.0 |
| NA |
|