| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.56507424 |
| We found that the association of synaptotagmin 1 with SNAP 25 in brain homogenates of snapin mutant mice is impaired . 0.56507424^^^ |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| They compete with alpha SNAP for binding to the core complex but not with other interacting molecules , including synaptotagmin 1 , suggesting that the complexins regulate the sequential interactions of alpha SNAP and synaptotagmins with the SNAP receptor during exocytosis . . ^^^ |
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| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| Our immunoblot studies showed that ( 1 ) N CAM ( gp 180 ) immunoreactivity decrease with development ; ( 2 ) the expression of synaptotagmin 1 and SNAP 25 increases as development progresses , and ( 3 ) synaptotagmin 1 and SNAP 25 show a shift in subcellular localization ( from synaptic vesicle to synaptic membrane ) during development , thereby indicating that these proteins are first identified in a vesicular fraction . ^^^ |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| Using immunofluorescence microscopy , we found that cultured pinealocytes readily expressed all synaptic membrane proteins investigated , i . e . , synaptophysin , synaptotagmin 1 , synaptobrevin 2 , syntaxin 1 and SNAP 25 . ^^^ |
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| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| Membrane staining for SNAP 25 and weak cytoplasmic labelling for both synaptotagmin 1 and secretogranin 2 were detected in the intermediate lobe . ^^^ |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| A Csp binding site on the P / Q type calcium channel is thus located within the 200 residue synaptic protein interaction site that can also bind syntaxin 1 , SNAP 25 , and synaptotagmin 1 . ^^^ |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| The biochemical consequences of SNARE protein phosphorylation included a reduced interaction between SNAP 25 and phosphorylated syntaxin 4 and an enhanced interaction between phosphorylated syntaxin 1A and the synaptic vesicle protein synaptotagmin 1 , a potential Ca2+ sensor in triggering synaptic vesicle exocytosis . ^^^ |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| Co immunoprecipitation experiments were consistent with the hypothesis that both N and P / Q type calcium channels , but not L type channels , are associated with the 7S complex containing syntaxin 1 , SNAP 25 , VAMP and synaptotagmin 1 or 2 . ^^^ |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| Other known SNARE and SNARE associated proteins such as vesicle associated membrane protein ( VAMP ) / synaptobrevin 2 , SNAP 25 , synaptophysin , or synaptotagmin 1 could not be immunochemically detected in platelet membrane preparations . ^^^ |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| Here we demonstrate the astrocytic expression and organellar association of a large variety of synaptic proteins ( synaptobrevin 2 , synaptotagmin 1 , synaptophysin , rab3a , synapsin 1 , SNAP 25 , and syntaxin 1 ) and also of the ubiquitous cellubrevin . ^^^ |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemistry in combination with confocal laser microscopy showed the presence of synaptosomal associated protein of 25 kDa ( SNAP 25 ) , syntaxin , cysteine string protein ( CSP ) , synuclein , synapsin 1 , synapsin I / II , synaptotagmin 1 , synaptotagmin I / II , synaptophysin , and synaptobrevin 2 like immunoreactivity ( LI ) in VAChT containing C terminals . ^^^ VAChT containing varicosities in the Renshaw cell area , with a probable origin from motoneuron axon collaterals , exhibited CSP , synapsin I / II , and synaptobrevin 1 LI , but not SNAP 25 , syntaxin , synuclein , synapsin 1 , synaptotagmin 1 , synaptotagmin I / II , synaptophysin and synaptobrevin 2 LI . ^^^ |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| The tissues were fixed , cryoprotected , frozen , cut in 15 micrometer sections , and processed immunohistochemically using antibodies against synaptophysin , synaptotagmin 1 , or SNAP 25 . ^^^ |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| For synaptotagmin 1 , several putative targets have been identified , including the SNARE proteins syntaxin and SNAP 25 . ^^^ |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| So far palmitoylation of SNAP 25 and synaptotagmin 1 have been described in vivo . ^^^ |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| METHODS : Levels of mRNAs encoding four synaptic vesicle proteins ( synaptotagmin 1 [ p 65 ] , rab3a , synaptobrevin 1 , and synaptobrevin 2 ) and two synaptic plasma membrane proteins ( syntaxin 1A and SNAP 25 ) were measured postmortem in the left superior temporal gyrus from elderly ( 58 95 years ) schizophrenic patients ( n = 14 ) and age matched control subjects ( n = 9 ) . ^^^ RESULTS : There were significant negative correlations between age and levels of synaptotagmin 1 ( p 65 ) , rab3a , synaptobrevin 1 , SNAP 25 , and syntaxin 1A mRNAs in schizophrenic patients ( . 692 < r < . 517 , . 003 < p < . 030 ) but not in control subjects . ^^^ |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| In these investigations we found that the same treatment induced accumulation over 2 fold of other proteins in the secretory pathway , including synaptosomal associated protein of 25 kDa ( SNAP 25 ) , synaptotagmin 3 , synaptobrevin , synaptophysin , and cyclophilin B , and did not affect accumulation of others , including synaptotagmin 1 , calnexin , and glucose regulated protein 94 . ^^^ |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| After decentralization of the ganglia several of the synaptic vesicle proteins ( synaptotagmin 1 , synaptophysin , SNAP 25 , CLC and GAP 43 ) were increased in the iris nerve terminal network , but with different time patterns , while TH immunoreactivity had clearly decreased . ^^^ |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| In addition , the C ( 2 ) domains of RIM associate with SNAP 25 and synaptotagmin 1 . ^^^ The interactions of the C ( 2 ) domains of RIM with SNAP 25 and synaptotagmin 1 are modulated by Ca ( 2+ ) . ^^^ Thus , in the presence of Ca ( 2+ ) ( EC ( 50 ) = approximately 75 microm ) the interaction with synaptotagmin 1 is increased , whereas SNAP 25 binding is reduced . ^^^ |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| The phosphorylation inhibited the interaction of L ( 2 3 ) with SNAP 25 and synaptotagmin 1 , which were plasma membrane soluble N ethylmaleimide sensitive factor attachment protein ( SNAP ) receptor ( SNARE ) proteins and were required for efficient neurotransmitter release . ^^^ |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| Tubulin and synaptotagmin 1 were co precipitated from the synaptosome extract with monoclonal antibodies to tubulin and SNAP 25 ( synaptosome associated protein of 25 kDa ) , indicating the presence of tubulin / synaptotagmin 1 complex and tubulin binding to synaptotagmin 1 in SNARE ( soluble N ethylmaleimide sensitive factor attachment protein receptor ) complexes . ^^^ |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| SNAP 25 and synaptotagmin 1 function in Ca2+ dependent reversible docking of granules to the plasma membrane . ^^^ In the docking assay , addition of Ca2+ induces the formation of a SNAP 25 Synaptotagmin 1 complex . ^^^ These results show that Ca2+ modulates dynamic docking of granules to the plasma membrane and that this process is due to a Ca2+ dependent interaction between SNAP 25 and Synaptotagmin 1 . . ^^^ |
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| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| Synaptotagmin 1 has been implicated as the Ca2+ sensor because it binds Ca2+ , and this enhances its binding to syntaxin , SNAP 25 and phospholipids in vitro . ^^^ Secretion elicited from permeabilised cells by microM Ca2+ was accompanied , with almost identical Ca2+ dependencies , by changes in synaptotagmin 1 , SNAP 25 , syntaxin and synaptobrevin that rendered them less susceptible to trypsin . ^^^ Removal of nine C terminal residues from SNAP 25 by botulinum toxin A reduced both exocytosis and the SNAREs ' acquisition of trypsin resistance but did not alter the Ca2+ sensitivity , except for synaptotagmin 1 . ^^^ These data support a model whereby Ca2+ is sensed , probably by synaptotagmin 1 , and the signal passed to syntaxin and SNAP 25 before they interact with synaptobrevin . . ^^^ |
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| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| In this work , neuroblastoma cells expressing synaptotagmin 1 , a protein shown to be bound by BoNT / A , were used to determine whether specific gangliosides were necessary for BoNT / A activity as measured by synaptosomal associated protein of 25 kDa ( SNAP 25 ) cleavage . ^^^ Whereas both cell lines expressed synaptotagmin 1 , SNAP 25 cleavage was not observed in the absence of complex gangliosides . ^^^ |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| Our study reveals that , in the absence of calcium , native synaptotagmin 1 binds the t SNARE heterodimer , formed from syntaxin and SNAP 25 . ^^^ |
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| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| Using indirect immunofluorescence microscopy , we analyzed the spatial and temporal distribution of 11 important membrane and membrane associated synaptic proteins ( syntaxin 1 / 3 , SNAP 25 , synaptobrevin 2 , synaptogyrin , synaptotagmin 1 , SV2A , SV2B , Rab3A , clathrin light chains , CSP and neuroligin 1 ) during synaptogenesis . ^^^ |
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| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| The roles of SNARE proteins , i . e . neuronal Synaptobrevin ( n Syb ) , SNAP 25 and Syntaxin 1A ( Syx 1A ) , and Synaptotagmin 1 ( Syt 1 ) in synaptic transmission have been studied in situ using mutant embryos or larvae that lack these molecules or have alterations in them . ^^^ |
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| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| We have also cloned SNAP 25 in Aplysia and show that it binds synaptotagmin 1 C2B beta with a higher affinity than synaptotagmin 1 C2B alpha . ^^^ |
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| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| We also found that palmitate was stably associated with both synaptotagmin 1 and SNAP 25 and that rapid neuronal depolarization did not affect palmitate turnover on these proteins . ^^^ |
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| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| The SV2B synaptotagmin 1 complex also contained the synaptic t SNARE proteins , syntaxin 1 and SNAP 25 , suggesting that SV2B may participate in exocytosis by modulating the interaction of synaptotagmin 1 with t SNARE proteins . ^^^ |
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| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| The presynaptic membrane protein SNAP 25 colocalized with DA terminals , but several other presynaptic proteins tested , including synaptotagmin 1 , synapsin , bassoon , syntaxin , and synaptogyrin , appeared not to be associated with DA neurons . ^^^ |
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| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| HIP 14 shows remarkable substrate specificity for neuronal proteins , including SNAP 25 , PSD 95 , GAD 65 , synaptotagmin 1 , and htt . ^^^ |
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| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| Moreover , we find that the E170A / Q177A SNAP 25 mutation does not alter interactions between the SNAREs and the Ca2+ sensor synaptotagmin 1 , but severely impairs SNARE complex assembly . ^^^ |
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| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| Thus , the C terminus of SNAP 25 , which links synaptotagmin 1 to the SNARE complex , may represent a target of Gbetagamma for presynaptic inhibition . . ^^^ |
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| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| An investigation of the onset of the appearance of mRNA and protein in dissociated cultures of neurons from mouse hippocampus or from chick retina has shown an early abundance of proteins involved in exocytosis , such as syntaxin 1 , SNAP 25 , and synaptotagmin 1 , whereas dynamin 1 , a protein necessary for clathrin mediated endocytosis , can be detected only after neurons have established contacts with neighboring cells . ^^^ |
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| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| The RIM C2A domain does not contain the full complement of aspartate residues that commonly mediate Ca2+ binding at the top loops of C 2 domains , and has been reported to interact with SNAP 25 and synaptotagmin 1 , two proteins from the Ca2+ dependent membrane fusion machinery . ^^^ Moreover , little binding of the RIM 2 C2A domain to SNAP 25 and to the C 2 domains of synaptotagmin 1 was detected by NMR experiments , suggesting that as yet unidentified interactions of the RIM C2A domain mediate its function . ^^^ |
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| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| In the present study , 1 discovered that dithreonine residues ( Thr 15 and Thr 16 ) at the intravesicular domain of mouse Syt 1 are post translationally modified by a complex form of O linked sugar ( i . e . the addition of sialic acids ) in PC 12 cells and that the O glycosylation of Syt 1 in COS 7 cells depends on the coexpression of vesicle associated membrane protein 2 ( VAMP 2 ) / synaptobrevin . 1 also showed that a transmembrane domain of Syt 1 directly interacts with isolated VAMP 2 , but not VAMP 2 , in the heterotrimeric SNARE ( SNAP receptor ) complex ( vesicle SNARE , VAMP 2 , and two target SNAREs , syntaxin IA and SNAP 25 ) . ^^^ |
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| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| An account of these domains and their functions is given here for the four best described fusion systems : influenza HA , sendai virus F 1 , HIV gp120 / 41 and the neuronal SNARE core composed of synaptobrevin ( syn ) , syntaxin ( stx ) and the N and C termini of SNAP 25 ( sn 25 ) , together with the Ca ( 2+ ) binding protein synaptotagmin ( syt ) . ^^^ |
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| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| The C 2 domains of syt also interact with syntaxin and SNAP 25 , two components of a conserved membrane fusion complex . ^^^ |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| All tested isoforms , except Syt 12 and Syt 13 , bound the target SNARE heterodimer comprising syntaxin 1 and SNAP 25 ( 25 kDa synaptosome associated protein ) . ^^^ |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| In neurons the core SNARE ( soluble N ethylmaleimide sensitive factor attachment protein receptor ) complex , composed of syntaxin ( Stx ) , SNAP 25 , and VAMP 2 , mediates vesicle fusion , whereas calcium regulation is thought to be accomplished by the synaptotagmin ( Syt ) family , some of which exhibit calcium dependent binding to syntaxin and SNAP 25 . ^^^ |
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| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| Ca ( 2+ ) promotes the interaction of syt with anionic phospholipids and the target membrane SNAREs ( t SNAREs ) SNAP 25 and syntaxin . ^^^ Here , we have used a defined reconstituted fusion assay to determine directly whether syt t SNARE interactions couple Ca ( 2+ ) to membrane fusion by comparing the effects of Ca ( 2+ ) syt on neuronal ( SNAP 25 , syntaxin and synaptobrevin ) and yeast ( Sso1p , Sec9c and Snc2p ) SNAREs . ^^^ Ca ( 2+ ) syt drove assembly of SNAP 25 onto membrane embedded syntaxin , providing direct evidence that Ca ( 2+ ) syt alters t SNARE structure . . ^^^ |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21579 and P60880 |
Pubmed |
SVM Score :0.0 |
| NA |
|