| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| The current review will fill the gap in our understanding of their role in cellular signalling networks , and by improving this understanding , an integrated picture regarding the normal function of tuberin and hamartin is beginning to emerge . . ^^^ |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| Overexpression of the Tuberin Hamartin heterodimer inhibits Rheb mediated S6K1 activation , suggesting that Tuberin functions as a Rheb GTPase activating protein ( GAP ) . ^^^ Tuberous sclerosis complex gene products , Tuberin and Hamartin , control mTOR signaling by acting as a GTPase activating protein complex toward Rheb . ^^^ Moreover , in vitro studies reveal that Tuberin , when associated with Hamartin , acts as a Rheb GTPase activating protein . ^^^ |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| Together , these genetic , biochemical and cell biological studies have demonstrated that the tuberin hamartin complex inhibits target of rapamycin ( TOR ) signaling by acting as a GTPase activating protein for the Ras related small G protein Rheb . . ^^^ |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| Signaling through mTOR is also controlled by upstream members of the pathway such as the Ras homolog enriched in brain ( Rheb ) , a GTPase that activates mTOR , and tuberin ( also known as TSC 2 ) , a GTPase activating protein , which , with its binding partner hamartin ( also known as TSC 1 ) , acts to repress mTOR . ^^^ |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| The complex of AS 250 with KIAA 1219 is notably similar to the important regulatory complex of the protein tuberin with hamartin ( the tuberous sclerosis complex ) , in the size of its subunits , the location of the GAP domain , and its phosphorylation by Akt . ^^^ |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| We show that hamartin colocalizes with hypophosphorylated tuberin at the membrane , where tuberin exerts its GTPase activating protein ( GAP ) activity to repress Rheb signaling . ^^^ |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| Loss of TSC 2 GAP activity or disruption of the TSC1 / TSC2 complex dysregulates S6K1 activation , which leads to abnormal cell proliferation associated with LAM disease . . ^^^ The full length TSC 2 was necessary for inhibition of S 6 hyperphosphorylation and DNA synthesis in LAM cells , as demonstrated by co microinjection of the C terminus , which contains the GTPase activating protein homology domain , and the N terminus , which binds TSC 1 . ^^^ |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| The TSC 2 gene product , named tuberin , is a 1784 amino acid protein that shows a small stretch of homology to the GTPase activating protein rap1GAP . ^^^ |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| Intriguingly , the TSC 2 product , tuberin , has an area of sequence homology with the GTPase activating protein rap1GAP , suggesting a possible mechanism for its role in regulating cellular growth . . ^^^ |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| The 5 . 5 kb TSC 2 transcript is widely expressed , and its protein product , tuberin , has a region of homology to the GTPase activating protein GAP3 . . ^^^ |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| We did not detect an increase in mutations either in the GTPase activating protein ( GAP ) related domains of TSC 2 or in the activating domains that have been identified in rat tuberin . ^^^ |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| TSC 2 encodes tuberin , a putative GTPase activating protein for rap 1 and rab 5 . ^^^ |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| Individuals with tuberous sclerosis complex ( TSC ) develop astrocytoma like tumors resulting from mutations in the TSC 2 protein , tuberin , which is hypothesized to function as a Rap 1 GTPase activating protein ( GAP ) . ^^^ |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| The TSC 2 gene product , tuberin , encodes a GTPase activating protein ( GAP ) domain , which regulates the activity of Rap 1 in vitro . ^^^ |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| Tsc 2 possesses a domain that shares homology with the GTPase activating protein ( GAP ) domain of Rap 1 GAP , suggesting that a GTPase might be the physiological target of Tsc 2 . ^^^ Here we show that the small GTPase Rheb ( Ras homologue enriched in brain ) is a direct target of Tsc 2 GAP activity both in vivo and in vitro . ^^^ Point mutations in the GAP domain of Tsc 2 disrupted its ability to regulate Rheb without affecting the ability of Tsc 2 to form a complex with Tsc 1 . ^^^ |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| Finally , coexpression of a human TSC 2 cDNA harboring a disease associated point mutation in the GAP domain , failed to stimulate Rheb GTPase activity or block Rheb activation of S6K1 . . ^^^ |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| Rheb GTPase is a direct target of TSC 2 GAP activity and regulates mTOR signaling . ^^^ Here we report that TSC 2 is a GTPase activating protein ( GAP ) toward Rheb , a Ras family GTPase . ^^^ |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| TSC 2 : filling the GAP in the mTOR signaling pathway . ^^^ It has recently been shown that TSC 2 has GTPase activating protein ( GAP ) activity towards the Ras family small GTPase Rheb ( Ras homolog enriched in brain ) , and TSC1 / 2 antagonizes the mTOR signaling pathway via stimulation of GTP hydrolysis of Rheb . ^^^ |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| Recent studies of Drosophila and mammalian cells demonstrate that the TSC 1 TSC2 complex functions as GTPase activating protein against Rheb a Ras like small GTPase , which in turn regulates TOR signaling in nutrient stimulated cell growth . ^^^ |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| Biochemical and functional characterizations of small GTPase Rheb and TSC 2 GAP activity . ^^^ Recent studies have demonstrated that TSC 2 displays GAP ( GTPase activating protein ) activity specifically towards the small G protein Rheb and inhibits its ability to stimulate the mTOR signaling pathway . ^^^ Rheb and TSC 2 comprise a unique pair of GTPase and GAP , because Rheb has high basal GTP levels and TSC 2 does not have the catalytic arginine finger found in Ras GAP . ^^^ We found that Arg 15 , a residue equivalent to Gly 12 in Ras , is important for Rheb to function as a substrate for TSC 2 GAP . ^^^ In addition , we identified asparagine residues essential for TSC 2 GAP activity . ^^^ |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| Recent biochemical analysis revealed that tuberous sclerosis complex ( TSC ) , a GTPase activating protein ( GAP ) , deactivates Rheb and that phosphatidylinositol 3 ' kinase ( PI3k ) Akt / PKB kinase pathway activates Rheb through inhibition of the GAP mediated deactivation . ^^^ |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| The most exciting advances in the tuberous sclerosis complex ( TSC ) field occurred in 1993 and 1997 with the cloning of the TSC 2 and TSC 1 genes , respectively , and in 2003 with the identification of Rheb as the target of tuberin ' s ( TSC 2 ) GTPase activating protein ( GAP ) domain . ^^^ |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| TSC 1 is known to stabilize TSC 2 by forming a complex with TSC 2 , which is a GTPase activating protein for the Rheb small GTPase . ^^^ |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| Tsc 1 and Tsc 2 proteins form a complex and serve as a GTPase activating protein ( GAP ) for Rheb , a GTPase regulating a downstream kinase , mTOR . ^^^ |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| We have used finger printing methods and hybridization to produce a 1 . 7 Mb overlapping clone map covering the TSC 1 candidate region , with a single gap of 20 kb . ^^^ |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| Loss of function of the TSC 1 TSC2 complex , which acts as a Rheb GAP , yields constitutive , unrestrained signaling from the cell growth machinery comprised of Rheb , mTOR , and S6K . ^^^ |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| Both protein products of TSC 1 and TSC 2 form an intracellular complex exerting GTPase activating ( GAP ) activity towards a small G protein , Ras homologue enriched in brain ( Rheb ) . ^^^ |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92574 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
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