Pubmed abstracts for Protein-Protein Interaction search result :


Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.53945347
Incubation of rap1A protein with ras GAP does not accelerate the rate of GTP hydrolysis by p21rap1A . 0.53945347^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
We have purified an 88 kd GTPase activating protein ( GAP ) , specific for the rap1 / Krev 1 gene product , from bovine brain . ^^^ Molecular cloning of a GTPase activating protein specific for the Krev 1 protein p21rap1 . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Both ras and Krev 1 proteins have identical effector domains ( ras residues 32 to 40 ) , which are required for biological activity and for the interaction of Ras p 21 with Ras GTPase activating protein ( GAP ) . ^^^ Replacement of these residues in Ras p 21 with the corresponding Krev 1 p 21 amino acids resulted in ras proteins which were impaired biologically or reduced in their affinity for in vitro GAP binding . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
The protein product of Krev 1 , Rap1A p 21 , is identical to Ras p 21 proteins in the region where interaction with guanosine triphosphatase ( GTPase ) activating protein ( GAP ) is believed to occur . ^^^ The ability of Rap1A p 21 to bind tightly to GAP may account for Krev 1 suppression of transformation by ras . ^^^ Inhibition of GTPase activating protein stimulation of Ras p 21 GTPase by the Krev 1 gene product . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
In microcystin transformed cells , PI3K , MAPKAPK 2 , Akt , cyclin D 1 and cyclin D 3 in the Akt pathway ; IQGAP 2 , RabGTPase , Rap1GAP , RasGAP , R Ras , Krev 1 and TC 21 of the Ras GTP / GDP protein family ; and A Raf , B Raf and PAK in the Ras / MAPK pathway were all markedly upregulated . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
The purification of a Rap 1 GTPase activating protein from bovine brain cytosol . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
However , it copurified with the cytosolic form of rap 1 GAP and was more efficient at stimulating the GTPase activity of the rap 1 protein ; this 55 kD polypeptide , that is recognized by an antibody raised against rap 1 GAPm , likely represents a degraded and soluble form of the full size 89 kD molecule . ^^^ In bovine brain membranes , a weak GAP activity toward the rap2A protein was also detected ; however , it was also attributable to the membrane associated rap 1 GAPm . ^^^ Thus , it appears that a single rap GAP protein , complete or degraded , is able to stimulate the GTPase activity of both rap 1 and rap 2 proteins . . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
This rap2b GTPase activating protein ( GAP ) activity is not immunoreactive with antibodies specific for rap 1 GAP or ras GAP , yet displays limited GTPase stimulatory activity toward rap 1 . ^^^ This result differs from the previously described rap 1 GAP which is highly specific for rap 1 . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
In this paper , we describe the enzymatic properties of several mutated Rap1A and chimeric Ras / Rap1A ( or 1B ) proteins which lead to the following conclusions : ( 1 ) the GAP 3 dependent activation of both Rap1A and 1B GTPases requires Gly 12 , but neither Thr 61 nor Gln 63 ; ( 2 ) residues 64 to 70 of the Rap 1 GTPases are sufficient to determine their specificities for GAP 3 ; and ( 3 ) residues 61 to 65 of the Ras GTPases are sufficient for determining their specificities for GAP 1 . ^^^ Thus , the domains of the Ras or Rap 1 proteins that determine whether their signals are attenuated by GAP 1 or GAP 3 are distinct from the N terminal domain ( residues 21 to 54 ) that determines whether their signals are oncogenic or antioncogenic . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Rsr 1 and Rap 1 GTPases are activated by the same GTPase activating protein and require threonine 65 for their activation . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Furthermore , the ability of a cytosolic rap 1 GTPase activating protein to stimulate neutrophil rap 1 GTP hydrolysis was not modified by phosphorylation . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Neither recombinant Rap1A nor neutrophil Rap 1 protein GTPase activity could be stimulated by recombinant Ras GAP at a concentration 25 fold higher than that required to hydrolyze 50 % of H Ras bound GTP under similar conditions . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Its N terminal domain is highly homologous to a human Rap 1 GTPase activating protein ( GAP ) , and a fusion protein of this domain ( SpanN ) indeed exhibited GAP activity for Rap1 / Rsr1 but not for Ras or Rho in vitro . ^^^ Unlike the human Rap 1 GAP , however , SpanN also exhibited GAP activity for Ran , so far the only known Ras related GTPase in the nucleus . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
The GAP 1 ( also called Ras GAP ) is highly specific for Ras and does not stimulate the intrinsic GTPase activity of Rap 1 or Rho . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Baculovirally expressed p 130 ( SPA 1 ) exhibited GTPase activating protein ( GAP ) activity for Rap 1 and Rap 2 , but not for Ras , Rho , Cdc 42 , Rac , and Ran , with comparable specific activity to the rap1GAP gene product ( p85 / 95 ( rap1GAP ) ) . ^^^ These results suggested that products of SPA 1 and rap1GAP genes , albeit comparable GAP activity for Rap 1 and Rap 2 , functioned in the distinct contexts depending on cell types and / or states . . ^^^ Human SPA 1 gene product selectively expressed in lymphoid tissues is a specific GTPase activating protein for Rap 1 and Rap 2 . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
We have isolated cDNAs encoding a GAP for the Drosophila Rap 1 GTPase . ^^^ Biological characterization of Drosophila Rapgap 1 , a GTPase activating protein for Rap 1 . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
The TSC 2 gene on chromosome 16 encodes a 1784 amino acid tumor suppressor protein , tuberin , that functions as a GTPase activating protein for Rap 1 , a member of the superfamily of Ras related proteins . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
TSC 2 encodes tuberin , a putative GTPase activating protein for rap 1 and rab 5 . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
In this study we have shown that SPA 1 interferes with Rap 1 activation by membrane targeted C3G , C3G F , in 293T cells through the GTPase activating protein ( GAP ) activity . ^^^ Rap 1 GTPase activating protein SPA 1 negatively regulates cell adhesion . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Modulation of rap activity by direct interaction of Galpha ( o ) with Rap 1 GTPase activating protein . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Rap1GAP ( for Rap 1 GTPase Activating Protein ) is an 89 kD protein that highly stimulates the intrinsic GTPase activity of the small GTP binding protein Rap 1 . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Purification of baculovirus produced Rap 1 GTPase activating protein . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
A recombinant protein consisting of the GAP related domain of p 98 fused to maltose binding protein stimulated GTPase activity of R Ras , and showed a weak effect on that of Ha Ras but not that of Rap 1 or Rho . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
TSC 2 is predicted to encode a 1784 amino acid tumor suppressor protein that may function , in part , as a GTPase activating protein for Rap 1 . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Tuberin , the human TSC 2 gene product , has a small region of homology with rap1GAP and stimulates rap 1 GTPase activity in vitro , suggesting that one of its cellular roles is to function as a GTPase activating protein ( GAP ) . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Sipa 1 , previously called Spa 1 , is transcriptionally induced in the murine lymphoid cells following mitogenic stimulation and encodes a protein with a domain related to Rap 1 GTPase activating protein ( Rap1GAP ) at the N terminus and to PEST sequences followed by a leucine zipper motif at the C terminus . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Inhibition of Rap 1 activation by a dominant negative Rap 1 or SPA 1 , a Rap 1 GTPase activating protein , abrogates LFA 1 ICAM 1 mediated adhesive interactions with antigen pulsed APC and the subsequent T cell receptor triggering and interleukin 2 production . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
RESULTS : We found that the PSD 95 family proteins interact via their GK domains with SPA 1 like protein ( SPAL ) , a GTPase activating protein ( GAP ) that is specific for Rap 1 . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
However , overexpression of a RAP 1 GTPase activating protein ( RAP1GAP ) , which efficiently clamped cellular RAP 1 in the inactive GDP bound form , did not affect A ( 2A ) agonist mediated MAP kinase stimulation . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Overexpression of the Rap 1 specific GTPase activating protein , SPA 1 , or inactivated form of Rap 1 ( N17Rap1 ) blocked phorbol ester stimulated adhesion of Jurkat cells to fibronectin ( alpha ( 4 ) beta ( 1 ) ) and ICAM 1 ( alpha ( L ) beta ( 2 ) ) . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Moreover , blocking the activation of Rap 1 and Rap 2 via the expression of a Rap specific GTPase activating protein significantly reduced the ability of B cells to migrate toward SDF 1 . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Inhibition of Rap 1 either by Rap GTPase activating protein ( RapGAP ) or the Rap 1 binding domain of RalGDS abolished both Mn ( 2+ ) and KIM 185 ( anti LFA 1 ) induced LFA 1 mediated cell adhesion to intercellular adhesion molecule 1 . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Structure function analyses indicate that the first 74 amino terminal residues of Rap1GAP , a region distinct from the catalytic core domain responsible for the GAP activity toward Rap 1 , is required for this interaction . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Our results suggest that CD 28 mediated Rap 1 GTPase activating protein activation can help explain the augmentation of ERKs during CD 28 costimulation . . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Consistently , overexpression of putative Rap dominant negative mutant RapN 17 or the physiologic inhibitor of Rap 1 , the Rap GTPase activating protein RapGAP , augmented the Elk 1 response in CD45RO ( + ) T cells . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Specific phosphatidylinositol 3 kinase ( PI3K ) inhibitors suppressed both LFA 1 activation and Rap1GTP generation , and abrogation of Rap1GTP by retroviral over expression of a specific Rap 1 GTPase activating protein , SPA 1 , totally inhibited the LFA 1 / ICAM 1 mediated cell adhesion . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
In contrast , neither full length RGS 14 nor the isolated RBD domain is a GAP for Rap 1 . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Enforced expression of either wild type Rap 1 or the GTPase ( GAP ) resistant mutant Rap 1 V12 failed to override stromal inhibition , suggesting that the inhibitory mechanism does not involve GAP up regulation but rather may target upstream guanine nucleotide exchange factor ( GEF ) complexes . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Individuals with tuberous sclerosis complex ( TSC ) develop astrocytoma like tumors resulting from mutations in the TSC 2 protein , tuberin , which is hypothesized to function as a Rap 1 GTPase activating protein ( GAP ) . ^^^ Since we have previously reported that high grade astrocytomas frequently exhibit loss of tuberin expression or increased Rap 1 levels , we sought to determine whether there is a correlation between decreased tuberin Rap 1 GAP function or Rap 1 overexpression and tumor Rap 1 activity . ^^^ However , there was no correlation between tuberin expression or Rap 1 overexpression and the levels of Rap 1 activity in the tumors studied , suggesting that the increased Rap 1 activation is not the direct result of reduced tuberin Rap 1 GAP function or elevated Rap 1 protein expression . . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Using two different approaches , we now demonstrate that human E6TP1 exhibits GAP activity for Rap 1 and Rap 2 , confirming recent findings that a closely related rat homologue exhibits Rap specific GAP activity . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Indeed , introduction of a constitutively active Rap 1 as well as Epac induces integrin mediated cell adhesion , whereas inhibition of Rap 1 signalling by the introduction of Rap1GAP ( GTPase activating protein ) inhibits inside out activation of integrins . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
In the present study , we showed that SPA 1 , a Rap 1 GTPase activating protein ( GAP ) , was bound to a cytoskeleton anchoring protein AF 6 . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
The Tsc 2 tumor suppressor gene encodes the protein tuberin , a multi functional protein with sequence homology to the GTPase activating protein ( GAP ) for Rap 1 . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
The TSC 2 gene product , tuberin , encodes a GTPase activating protein ( GAP ) domain , which regulates the activity of Rap 1 in vitro . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Inhibition of Rap 1 by Spa 1 , a Rap 1 specific GTPase activating protein , abrogated chemokine stimulated lymphocyte rapid adhesion to endothelial cells under flow via intercellular adhesion molecule 1 . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
SPA 1 ( signal induced proliferation associated gene 1 ) is a principal Rap 1 GTPase activating protein in hematopoietic progenitors . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
In the paper by Ishida et al . in this issue of Cancer Cell , the authors report the results of targeted inactivation of a Rap 1 specific GTPase activating protein ( GAP ) gene , called SPA 1 , in mice . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
To do this , we blocked the activation of endogenous Rap 1 and Rap 2 by expressing the Rap specific GTPase activating protein RapGAPII . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
SPA 1 is a principal Rap 1 GTPase activating protein in the hematopoietic progenitors and peripheral T cells , and SPA 1 deficient mice develop a spectrum of myeloproliferative stem cell disorders of late onset . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
In lymphohematopoietic tissues , SPA 1 is a principal Rap 1 GTPase activating protein . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Recently , AQP 2 binding proteins that directly regulate this trafficking have been uncovered : SPA 1 , a GTPase activating protein ( GAP ) for Rap 1 , and cytoskeletal protein actin . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Rap1GAP2 is a new GTPase activating protein of Rap 1 expressed in human platelets . ^^^ Rap1GAP2 is the first GTPase activating protein of Rap 1 found in platelets and is likely to have an important regulatory role in platelet aggregation . . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Recently , we have identified for the first time proteins which directly bind to AQP 2 : SPA 1 , a GTPase activating protein for Rap 1 , and cytoskeletal protein actin . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
To delineate T cell function in the absence of Rap 1 activity , we generated transgenic mice expressing Rap1GAP1 , a Rap 1 specific GTPase activating protein . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Studies in GH4C1 cells , with an expression vector for Rap 1 GTPase activating protein ( Rap 1 GAP1 ) , demonstrated reduced VIP induced MAPK activation , indicating that VIP dependent activation of the extracellular signal regulated kinase ( ERK ) pathway requires PKA Rap 1 signaling . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Here we identified a potent Rho GTPase activating protein ( GAP ) , RA RhoGAP , as a direct downstream target of Rap 1 in the neurite outgrowth . ^^^ RA RhoGAP has the RA and GAP domains and showed GAP activity specific for Rho , which was enhanced by the binding of the GTP bound active form of Rap 1 to the RA domain . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Recently , AQP 2 binding proteins which could regulate this trafficking have been discovered ; SPA 1 ( signal induced proliferation associated gene 1 ) , a GAP ( GTPase activating protein ) for Rap 1 , and the cytoskeletal protein actin . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
This domain shows some homology to the GAP domains for Rheb at the carboxy terminus of the protein tuberin and for Rap 1 in the protein Rap 1 GAP . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Human Rap1GAP , the GTPase activating protein ( GAP ) for the small GTPase Rap 1 , was recombinantly expressed in Escherichia coli , purified and crystallized using the hanging drop vapour diffusion method . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
The tumor suppressor protein Tsc 2 has a proposed GTPase activating protein ( GAP ) function for Rap 1 , providing a common mechanistic target for Tsc 2 and TPA . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
A biochemical search for AQP 2 interacting proteins led to the identification of PDZ domain containing protein , signal induced proliferation associated gene 1 ( SPA 1 ) which is a GTPase activating protein ( GAP ) for Rap 1 . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Rap 1 neither possesses a Gln nor does its cognate Rap GAP employ an Arg . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
The rap 1A protein has also been shown to block the interaction of ras with its GTPase activating protein in vitro , leading to speculation regarding its role in vivo . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Rap1A antagonizes the ability of Ras and Ras Gap to inhibit muscarinic K+ channels . ^^^ The binding of Rap1A to Ras GTPase activating protein ( GAP ) through this domain is a potential mechanism for explaining the observation that Rap1A can antagonize the ability of oncogenic Ras to transform cells . ^^^ We have made use of this system in the present paper to show that Rap1A is able to effectively block this inhibitory action of Ras GAP . ^^^ We observed that both Rap1A GDP and Rap1A guanosine 5 ' 3 O ( thio ) triphosphate ( GTP gamma S ) were able to block the inhibitory effect of Ras GAP upon channel activation . ^^^ A Rap1A Thr 35 Ala mutation which bound GTP gamma S did not prevent K+ channel inhibition by Ras GAP , suggesting that the antagonism by wild type Rap1A involves an interaction with GAP in the effector domain . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Seven other proteins , however , have greater than 50 % amino acid identity to ras and one of them , rap1A , has been shown to interact with the ras GTPase activating protein , ras GAP , and to inhibit ras function when overexpressed . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Here , we demonstrate that the abrogation of Rap 1 activation by SPA 1 , a Rap 1 specific GAP , inhibits activation of B Raf , MEK , Erk , and Akt in a murine hematopoietic cell line , Ton . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
We previously reported that the mice deficient for SPA 1 , a Rap 1 GTPase activating protein , developed hematopoietic stem cell disorders . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Of these , protein levels of RAP1GA1 ( RAP 1 GTPase activating protein 1 ) showed a trend of increase in BA 46 from bipolar brains , in keeping with mRNA transcript levels . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
Heterogeneous amino acids in Ras and Rap1A specifying sensitivity to GAP proteins . ^^^ Guanosine triphosphatase ( GTPase ) activity of Ras is increased by interaction with Ras GAP ( GTPase activating protein ) or with the GAP related domain of the type 1 neurofibromatosis protein ( NF 1 GRD ) , but Ras is not affected by interaction with cytoplasmic and membrane forms of Rap GAP ; Rap1A , whose effector function can suppress transformation by Ras , is sensitive to both forms of Rap GAP and resistant to Ras GAP and NF 1 GRD . ^^^ Residues 61 to 65 of Ras conferred Ras GAP sensitivity , but a larger number of Rap1A residues were required for sensitivity to cytoplasmic Rap GAP . ^^^ Thus , distinct amino acids of Ras and Rap1A mediate sensitivity to each of the proteins with GAP activity , and transforming potential of Ras and sensitivity of Ras to Ras GAP are at least partially independent properties . . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
We also analyzed the stimulated GTPase reaction of p21ras by the GTPase activating protein ( GAP ) and the GTPase reaction of Rap1A , a Ras related GTP binding protein , with similar approaches . ^^^ The corresponding results indicate that the GAP stimulated GTPase as well as the Rap1A catalyzed reaction seem to follow the same substrate assisted reaction mechanism . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
The TSC 2 product , tuberin , has a region of homology with the GTPase activating protein rap1GAP and stimulates the GTPase activity of rap1a and rab5a in vitro . ^^^
Interacting proteins: P62834 and P20936 Pubmed SVM Score :0.0
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