Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.8021733
Previous studies have suggested that GAP is phosphorylated by oncogenic viral Src ( 5 Src ) and that GAP is associated with a complex containing normal cellular Src ( c Src ) in vertebrate fibroblasts . 0.8021733^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.69401447
To examine molecular interactions between GAP and the two Src kinases , immunoprecipitates of Src or GAP prepared from cell lystates were resolved by gel electrophoresis and analyzed by an immunoblot procedure with antibodies to GAP or Src used as probes . 0.69401447^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.88728094
To further investigate the molecular basis for interactions between GAP and the Src kinases , we examined GAP association with and phosphorylation by a series of c Src and 5 Src mutants . 0.88728094^^^ Our previous studies showed that GAP complexes stably with normal cellular Src ( c Src ) , although its association with 5 Src is less stable . 0.53088559^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.93575957
Indeed , Src interacted with phosphorylated RasGAP fusion proteins ; immunodepletion of Src markedly decreased the recovery of the RasGAP associated kinase activity . 0.93575957^^^ When RasGAP is recruited to the betaPDGFR , it is phosphorylated and associates with Src . 0.63402179^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
In EMK cells , Sik binds GAP A . p65 , and this interaction is mediated by the Sik Src homology 2 domain . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Gap junction closure is prevented by tyrphostins , by dominant negative c Src , and in Src deficient cells . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Gap junctional conductance in cardiomyopathic hamsters : the role of c Src . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Intersectin Src homology 3 also inhibits CdGAP activity in GAP assays in vitro . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Mechanism of 5 Src and mitogen activated protein kinase induced reduction of gap junction communication . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Gap junction closure was prevented by tyrphostin 25 and by the selective c Src inhibitor , PP 2 . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Normal cells control the growth of neighboring transformed cells independent of gap junctional communication and SRC activity . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
ASAP 1 , a phospholipid dependent arf GTPase activating protein that associates with and is phosphorylated by Src . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Disruption of gap junctional communication ( measured by intercellular dye transfer ) in cultured fibroblasts by pp60v src is correlated with phosphorylation of the gap junction protein , connexin 43 ( cx 43 ) , on tyrosine . ^^^ Additionally , activation of ts pp60v src tyrosine kinase activity upon shift of cells to the permissive temperature was correlated with a rapid increase in the phosphorylated tyrosine content of cx 43 proteins and loss of gap junctional communication . ^^^ These combined results suggested that cx 43 is a substrate of pp60v src whose phosphorylation on tyrosine may be involved in the disruption of gap junctional communication observed in Rous sarcoma virus ( RSV ) transformed cells . . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Signalling proteins such as phospholipase C gamma ( PLC gamma ) or GTPase activating protein ( GAP ) of ras contain conserved regions of approximately 100 amino acids termed src homology 2 ( SH 2 ) domains . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
The interactions of the phosphotyrosine ( Tyr ( P ) ) containing proteins in basal and insulin stimulated 3T3 L 1 adipocytes with src homology 2 ( SH 2 ) domains from phosphatidylinositol 3 kinase ( PI3K ) , ras GTPase activating protein ( GAP ) , and phospholipase C gamma have been examined . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Ras GTPase activating protein ( GAP ) contains two Src homology 2 ( SH 2 ) domains which are implicated in binding to tyrosine phosphorylated sites in specific activated growth factor receptors and to a cytoplasmic tyrosine phosphorylated protein , p 62 . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Phosphopeptide mapping analysis revealed that 5 Src and normal cellular Src ( c Src ) phosphorylate tyrosine residues in bovine GAP at one major site and one minor site in vitro . ^^^ Activated Src tyrosine kinase phosphorylates Tyr 457 of bovine GTPase activating protein ( GAP ) in vitro and the corresponding residue of rat GAP in vivo . ^^^ GTPase activating protein ( GAP ) is a key regulator of the cellular Ras protein , which is implicated in oncogenic signal transduction pathways downstream of the viral Src ( 5 Src ) kinase . ^^^ Previous studies demonstrated that 5 Src induces tyrosine phosphorylation of GAP , suggesting that GAP may provide a biochemical link between 5 Src and Ras signaling pathways . ^^^ To determine the precise residues in GAP phosphorylated by Src kinases , we used a baculovirus / insect cell expression system for investigating in vitro phosphorylation of GAP . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
The Src homology 2 ( SH 2 ) domain is a noncatalytic region which is conserved among a number of signaling and transforming proteins , including cytoplasmic protein tyrosine kinases and Ras GTPase activating protein ( GAP ) . ^^^ In addition to binding soluble p 62 and p 130 , the SH 2 domains of 5 Src , GAP , and 5 Crk directly recognized these phosphotyrosine containing proteins which had been previously denatured and immobilized on a filter . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Src substrates p 36 , p 85 , p 120 , p 125 , the GTPase activating protein ( GAP ) , and several GAP associated proteins were phosphorylated on tyrosine in cells expressing the A , B , or C box deletion mutant . p 110 was highly phosphorylated in cells expressing the C box mutant , was weakly phosphorylated in cells expressing the B box mutant , and was not phosphorylated in cells expressing the A box mutant . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Through the use of isolated atrial cell membranes and genetically engineered GAP deletion mutants , the src homology regions ( SH 2 SH3 ) at the amino terminus of GAP have been identified as the domains responsible for this effect . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Transformation of cells with 5 src or activated transforming variants of c src or stimulation of cells with epidermal growth factor resulted in the stable association of GAP with two tyrosine phosphorylated cellular proteins of 64 kDa ( p 64 ) and 190 kDa ( p 190 ) . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
This domain is shared by a number of signal transduction proteins including nonreceptor tyrosine kinases such as Abl , Fps , Src , and Src family members , the transforming protein Crk , phospholipase C gamma 1 , PI 3 ( phosphatidylinositol ) kinase , and guanosine triphosphatase activating protein ( GAP ) . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
The interaction between p 62 and p 190 from lck transformed fibroblasts and GAP could be reconstituted in vitro using bacterial TrpE fusion proteins containing GAP Src homology 2 ( SH 2 ) domains . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
To determine whether connexin 43 , a major gap junction protein expressed in fibroblasts , is a target for the 5 src protein tyrosine kinase activity , we examined the phosphorylation state of connexin 43 in cells expressing variants of src . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
This predicts that overexpression of GAP would inactivate p21ras and block transformation of cells by src . ^^^ We report that overexpression of GAP in NIH 3T3 cells blocks transformation by pp60v src but not by 5 ras . ^^^ Susceptibility to transformation by 5 src is restored when GAP expression is lowered to levels comparable to that in control cells . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Here , we show that focus formation induced by c src was inhibited by approximately 80 % when cotransfected with a plasmid encoding full length GAP . ^^^ Cotransfection of the GAP C terminus coding sequences ( which encode the GTPase accelerating domain ) with c src or c src527F inhibited transformation more efficiently than did the full length GAP , while the GAP N terminus coding sequences had no effect on src transformation . ^^^ When cells transformed by c ras , c src , c src527F , or 5 src were transfected with GAP or the GAP C terminus sequence in the presence of a selectable marker , 40 to 85 % of the resistant colonies were found to be morphologically revertant . ^^^ Suppression of src transformation by overexpression of full length GTPase activating protein ( GAP ) or of the GAP C terminus . ^^^ The GAP C terminus induced reversion of each src transformed cell line more efficiently than the full length GAP , but this was not the case for reversion of c ras transformation . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Recently , PDGF and 5 Src have been shown to stimulate tyrosine phosphorylation of GAP , linking these pathways at the biochemical level . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
The action of 5 src on gap junctional permeability is modulated by pH . ^^^ Among the known consequences of pp60v src activity is the reduction in permeability of gap junctions , an effect that is counteracted by the calcium antagonist TMB 8 ( 8 N , N [ diethylamino ] octyl 3 , 4 , 5 trimethoxybenzoate ) . ^^^ These results suggest that TMB 8 does not inhibit 5 src action on junctional permeability by interfering with tyrosine phosphorylation of a protein crucial for closure of gap junction channels , but that the inhibition by low pH may be via this mechanism . . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Binding of SH 2 domains of phospholipase C gamma 1 , GAP , and Src to activated growth factor receptors . ^^^ Both PLC gamma 1 and GAP contain two adjacent copies of the noncatalytic Src homology 2 ( SH 2 ) domain . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
The hypothesis is supported by the fact that many cancer cells have some dysfunction in gap junctional intercellular communication , many tumor promoting chemicals and several oncogenes ( i . e . , ras , src , mos , neu , but not myc ) reduce gap junctional intercellular communication , and several growth factors ( i . e . , EGF , TGF beta , bovine pituitary extract ) inhibit gap junction function . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Cytoplasmic proteins that regulate signal transduction or induce cellular transformation , including cytoplasmic protein tyrosine kinases , p21ras GTPase activating protein ( GAP ) , phospholipase C gamma , and the 5 crk oncoprotein , possess one or two copies of a conserved noncatalytic domain , Src homology region 2 ( SH 2 ) . ^^^ In src transformed cells GAP forms heteromeric complexes , notably with a highly tyrosine phosphorylated 62 kDa protein ( p 62 ) . ^^^ Fusion proteins containing GAP or 5 Crk SH 2 domains complex with similar phosphotyrosine proteins from src transformed or EGF stimulated cells but with different efficiencies . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
This down regulation of gap junctional communication correlates with the activity of the gene product , the protein tyrosine kinase pp60c src . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
This down regulation of gap junctional communication correlates with the activity of the gene product , the protein tyrosine kinase pp60c src . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
The first 17 residues of the NH 2 terminal sequence of alpha ( MRSRSNSGVRLDSYARL . . . ) exhibit six identities and one conservative interchange with the transforming protein from the Rous sarcoma virus ( Schmidt Rupin strain ) provided a single gap is inserted in the src gene product . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Previous studies indicated that Tyr 457 of bovine GAP ( Tyr 460 of human GAP ) is the major site of phosphorylation by viral Src ( 5 Src ) kinase and epidermal growth factor receptor . ^^^ The finding that Tyr 457 in GAP is located immediately adjacent to Src homology 2 ( SH 2 ) and 3 ( SH 3 ) domains led us to investigate the possibility that this specific phosphorylation regulates protein protein interactions involving GAP . ^^^ Both wild type GAP and mutant GAPF 457 were tagged with the KT 3 epitope at the carboxyl terminus and were expressed in 5 Src transformed rat fibroblasts . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
In addition , this cell fraction contained the tyrosine kinases pp60c src and pp62c yes and the p21ras GTPase activating protein ( GAP ) . ^^^ These results are consistent with the possibilities that 1 ) GP IIb IIIa , pp60c src , pp62c yes , and GAP associate with a membrane skeleton fraction that contains spectrin , vinculin , and talin , 2 ) the association of GP IIb IIIa with adhesive ligand in a platelet aggregate causes components of the membrane skeleton to undergo altered association with cytoplasmic actin filaments , and 3 ) many of the proteins phosphorylated on tyrosine residues in activated platelets are components of the cytoskeleton . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Included were co precipitating proteins identified as the p 120 ras GTPase activating protein ( GAP ) and the p 62 protein that binds both to GAP and to a number of other tyrosine phosphorylated proteins having peptide regions homologous to the second domain of src . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Here we report that a 68K protein ( p 68 ) becomes tyrosine phosphorylated and physically associates with Src during mitosis in mouse fibroblasts . p 68 independently binds the Src SH 2 and SH 3 domains in vitro and both domains are required for p 68 phosphorylation and binding in vivo . p 68 is closely related to the p 62 protein that is associated with the Ras GTPase activating protein ( GAP ) and selectively binds , directly or indirectly , polyribonucleotides . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
That the association of p 62 with PLC gamma was direct and not mediated through binding of GAP p 62 to PLC gamma or to the EGF receptor ( and coprecipitation of the receptor with PLC gamma ) was demonstrated by ( 1 ) the inability to detect GAP in PLC gamma immunocomplexes or PLC gamma in GAP immunocomplexes , ( 2 ) the association of p 62 with PLC gamma in 5 src transformed cells in the absence of EGF stimulation , and ( 3 ) in vitro solution binding and direct blotting of p 62 with a glutathione S transferase fusion protein containing the Src homology 2 ( SH 2 ) domains of PLC gamma . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
When the two proteins were phosphorylated in vitro with purified c Src , they were both found to bind directly to the amino terminal SH 2 domain of GAP , although the RNA binding protein was found to have a weaker affinity . ^^^ These results indicate that two distinct 60 kDa proteins are substrates for the Src tyrosine kinase , one which binds RNA and the other which constitutes the major GAP associated 60 kDa phosphoprotein . . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Several laboratories have demonstrated a decrease in gap junctional communication in cells transformed by the src oncogene of the Rous sarcoma virus . ^^^ Since the presence of phosphotyrosine on Cx 43 is correlated with the down regulation of gap junctional communication , these results suggest that pp60v src regulates gap junctional gating activity via tyrosine phosphorylation of Cx43 . . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Our results suggest that GAP A . p62 may function as a docking protein and may mediate translocation of proteins , including GAP and CSK , to membrane or cytoskeletal regions upon c Src activation . . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
A domain ( amino acids 275 to 351 ) encompassing the Src homology region 3 ( SH 3 ) of GAP was found to be essential for GAP signaling . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Increased levels of p21ras GTP and enhanced DNA synthesis accompany elevated tyrosyl phosphorylation of GAP associated proteins , p 190 and p 62 , in c src overexpressors . ^^^ Further studies revealed that tyrosyl phosphorylation of p 190 and p 62 is not required for GAP complex formation , as equal amounts of p 190 and p 62 proteins could be detected in GAP complexes from wt and variant c src overexpressors both before and after EGF stimulation . ^^^ Taken together , these results suggest that one mechanism by which pp60c src may contribute to early events in the EGF induced mitogenic pathway in 10T1 / 2 fibroblasts is by increasing the level of GAP associated p 190 and p 62 tyrosyl phosphorylation , which in turn results in higher levels of p21ras GTP . . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Complex formation is likely to involve the src homology 2 ( SH 2 ) domains of GAP and autophosphorylated tyrosine residues of p93c fes , as deletion of the fes SH 2 domain did not abolish complex formation . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
The Ras GTPase activating protein ( GAP ) is an SH 3 domain binding protein and substrate for the Src related tyrosine kinase , Hck . ^^^ In this report , we provide evidence that GAP is an SH 3 domain binding protein and substrate for the Src related tyrosine kinase Hck , which has been implicated in the regulation of myeloid cell growth , differentiation , and function . ^^^ The WT and K269E forms of Hck also associated with GAP mutants lacking either the C terminal catalytic domain ( delta CAT ) or the Src homology region ( delta SH ) , indicating that these GAP domains are dispensable for complex formation . ^^^ Recombinant GST fusion proteins containing the Hck , Src , Fyn , or Lck SH 3 domains associated with full length GAP , delta CAT , and delta SH , all of which share an N terminal proline rich region resembling an SH 3 binding motif ( PPLPPPPPQLP ) . ^^^ These data implicate the Hck SH 3 domain in GAP interaction , and suggest a general function for the SH 3 domains of Src family kinases in recognition of GAP via its proline rich N terminal domain . . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
TXK shares 60 % amino acid homology with EMT and 57 % with BTK over the SH 3 , SH 2 ( Src homology ) and catalytic domains but unlike BTK , EMT and tec , it lacks Gap 1 homology and steroid hormone receptor homology in the N terminal region . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Coexpression of the N terminal region of GAP or of its isolated SH 3 ( Src homology 3 ) domain , but not its SH 2 domain , was also sufficient to suppress muscarinic receptor dependent focus formation . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Signaling molecules that appear to associate with the membrane skeleton in unstimulated platelets include pp60c src , pp62c yes , and GAP . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
METHODS : Immunodetection was used to compare the expression and tyrosine phosphorylation state of PLC gamma 1 , EGFR , phosphatidylinositol 3 kinase ( PI 3 kinase ) , ras guanosine triphosphatase activating protein ( GAP ) , and src homologous collagen like protein ( SHC ) in the postnatal rat intestine . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Upon stimulation , the tyrosine kinase PDGF receptor beta ( PDGFR beta ) autophosphorylates and forms a complex that includes SII 2 ( Src homology 2 ) domain containing proteins such as the phosphatidylinositol specific phospholipase C gamma , Ras GTPase activating protein ( GAP ) , and phosphatidylinositol 3 OH kinase . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
This is the product of an alternately spliced transcript of the human n chimerin gene encoding an N terminal SH 2 ( src homology 2 ) domain in addition to the phorbol ester receptor and GAP domains . alpha 1 and alpha 2 chimerin mRNAs were expressed differently . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Primary targets ( within 10 15 minutes ) were identified as PI 3 kinase , GAP , PLC gamma , src , and MAP kinase , substrates which are also activated upon growth factor activation of mitogenic receptor systems . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
We have used a transient expression system and mutant platelet derived growth factor ( PDGF ) receptors to study the binding specificities of the Src homology 2 ( SH 2 ) regions of the Ras GTPase activator protein ( GAP ) and the p 85 alpha subunit of phosphatidylinositol 3 kinase ( PI 3 kinase ) . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Since membrane association is critical for Ras function , we introduced the Ras membrane targeting signal ( a 19 residue peptide ending in CAAX , where C = cysteine , A = aliphatic amino acid , and 10 = any amino acid ) onto the GAP N terminal Src homology 2 and 3 and the C terminal catalytic domains ( designated nGAP / CAAX and cGAP / CAAX , respectively ) to determine the role of membrane association in GAP function . cGAP / CAAX and full length GAP / CAAX , but not GAP or nGAP / CAAX , exhibited potent growth inhibitory activity . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Immunoprecipitation experiments with specific antibodies against potential candidate proteins in the molecular mass range around 130kDa revealed positive results for the focal adhesion kinase FAK and the p 130 Src substrate while negative results were obtained for the GTPase activating protein GAP , the phospholipase C gamma 1 , the Janus kinase JAK 1 and vinculin . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
In contrast , Shc , phosphatidylinositol 3 kinase ( Pl3K ) , Grb 2 , and Ras GTPase activating protein ( GAP ) bound with different affinities to the bis or monophosphorylated peptides , while the Src family protein tyrosine kinase ( PTK ) Fyn did not bind specifically to any of the tested peptides . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Purified amino terminal Src homology 2 ( SH 2 ) domains of GAP , PLCgamma 1 and the p85alpha subunit of PI 3 kinase , as well as the carboxy terminal SH 2 domain of the latter protein and the unique SH 2 domain of Grb 2 , were injected into full grown , stage 6 Xenopus laevis oocytes . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
To examine the dichotomous role of Ras GAP predicted from these P34R results , we transfected both cell types with full length GAP , the C terminal catalytic domain ( cGAP ) , or N terminal Src homology domains ( nGAP ) . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Reduction of gap junctional communication in 5 src transformed cells is accompanied by tyrosine phosphorylation of the gap junction protein , connexin 43 ( Cx 43 ) . ^^^ These results provide further evidence for the direct involvement of 5 Src in tyrosine phosphorylation of Cx 43 and inhibition of gap junctional communication in 5 src transformed cells . . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
A comparison of the published crystal structures of the Src SH 2 domain and the N terminal SH 2 domain of SHP 2 complexed with high affinity peptides suggested that the alphaA 2 Gly of SHP 2 creates a gap which is filled by the side chain of the pY 2 residue of the bound peptide . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Employing antibodies specific to two p 85 isoforms , p85alpha and p85beta , we demonstrate that HTC IR cells express both p 85 isoforms , and these isoforms induce the formation of similar signaling complexes in response to insulin . p 60 70 , present in both alpha p85alpha and alpha p85beta immunoprecipitates , is a GAP associated protein , but is distinct from the p 68 src associated protein in mitosis ( Sam 68 ) by several criteria . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Regulation of p 190 Rho GAP by 5 Src is linked to cytoskeletal disruption during transformation . ^^^ Here , we have examined in detail the effects of a temperature dependent 5 Src protein on the regulation of p 190 RhoGAP , a GTPase activating protein ( GAP ) that has been implicated in disruption of the organised actin cytoskeleton , and addressed the dependence of 5 Src induced stress fibre loss on inhibition of Rho activity . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
In vitro binding experiments revealed that phosphorylation at Tyr 615 in EphA 8 mediates the preferential binding to Fyn SH 2 domain rather than Src and Ras GTPase activating protein ( Ras GAP ) SH 2 domains . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Functional role of c Src in gap junctions of the cardiomyopathic heart . ^^^ The functional consequences of tyrosine phosphorylation of connexin 43 in gap junction were assessed using transfected cells expressing constitutively active c Src . ^^^ It was found that constitutively active c Src diminished propagation of Ca ( 2+ ) waves in HEK 293 cells and reduced gap junctional conductance between pairs of cardiac myocytes . ^^^ We , therefore , conclude that during the progression of cardiac dysfunction in the cardiomyopathic heart , gap junctional communication is reduced via c Src mediated tyrosine phosphorylation of connexin43 . . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Autophosphorylation of the platelet derived growth factor ( PDGF ) receptor triggers intracellular signaling cascades as a result of recruitment of Src homology 2 domain containing enzymes , including phosphatidylinositol 3 kinase ( PI3K ) , the GTPase activating protein of Ras ( GAP ) , the protein tyrosine phosphatase SHP 2 , and phospholipase C gamma 1 ( PLC gamma 1 ) , to specific phosphotyrosine residues . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
A proline rich fragment of the NR2D subunit ( 2D ( 866 1064 ) ) bound to the Abl SH 3 domain but not to the SH 3 domains from Src , Fyn , Grb 2 , GAP , or phospholipase C gamma ( PLCgamma ) . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Functional studies demonstrated that CR 2 activation induced , within a brief span of 2 min , tyrosine phosphorylation of nucleolin and its interaction with Src homology 2 domains of the p 85 subunit of PI 3 kinase and of 3BP2 and Grb 2 , but not with Src homology 2 domains of Fyn and Gap . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
In response to insulin , the following proteins were rapidly phosphorylated on tyrosine residues : 1 ) insulin receptor substrates 1 ( IRS 1 ) , 2 , and 4 ; 2 ) phospholipase C isoenzyme gamma ; 3 ) the Ras GTPase activating protein ( GAP ) associated with Rho GAP and p 62 ( Src ) ; 4 ) the insulin receptor beta subunit ; 5 ) the p 85 subunits of phosphatidylinositol 3 kinase ( PI 3 kinase ) ; 6 ) the Src homology 2 alpha collagen protein ; 7 ) protein kinase B ; 8 ) mitogen activated protein ( MAP ) kinase 1 and 2 ; and 9 ) growth receptor bound protein 2 . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Phosphorylation of p 190 on Tyr 1105 by c Src is necessary but not sufficient for EGF induced actin disassembly in C3H10T1 / 2 fibroblasts . p 190 RhoGAP is a tyrosine phosphorylated protein that contains an N terminal GTP binding domain , a middle domain ( MD ) that mediates interaction with p 120 RasGAP and a C terminal GTPase activating protein ( GAP ) domain that is specific for the & Rgr ; family of small GTPases . ^^^ Our results support a model in which the p 190 MD negatively regulates the activity of the GAP domain and that c Src phosphorylation of Y 1105 is necessary , but insufficient on its own , for actin stress fiber disassembly . . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Differential actions of p60c Src and Lck kinases on the Ras regulators p 120 GAP and GDP / GTP exchange factor CDC25Mm . ^^^ It is known that the human Ras GTPase activating protein ( GAP ) p 120 GAP can be phosphorylated by different members of the Src kinase family and recently phosphorylation of the GDP / GTP exchange factor ( GEF ) CDC25Mm / GRF1 by proteins of the Src kinase family has been revealed in vivo [ Kiyono , M . , Kaziro , Y . & Satoh , T . ( 2000 ) J . ^^^ The phosphorylation of p 120 GAP by p60c Src inhibited its ability to stimulate the Ha Ras GTPase activity , whereas phosphorylation by Lck did not display any effect . ^^^ Our results suggest that phosphorylation by p60c Src and Lck is a selective process that can modulate the activity of p 120 GAP and CDC25Mm towards Ras proteins . . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
A previously unidentified Rho GTPase activating protein ( GAP ) domain containing protein was found in a yeast two hybrid screen for cDNAs encoding proteins binding to the Src homology 3 domain of Cdc 42 interacting protein 4 ( CIP 4 ) . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Src phosphorylation of connexin 43 on Tyr 247 and Tyr 265 disrupts gap junctional communication . ^^^ The mechanism by which 5 Src disrupts connexin ( Cx ) 43 intercellular gap junctional communication ( GJC ) is not clear . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
ASAP 1 ( ADP ribosylation factor [ ARF ] GTPase activating protein [ GAP ] containing SH 3 , ANK repeats , and PH domain ) is a phospholipid dependent ARF GAP that binds to and is phosphorylated by pp 60 ( Src ) . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
In our studies to elucidate the mechanism of Cx 43 channel gating by growth factors and to explore additional functions of gap junctions , we have identified three interacting partners of the C terminal tail of Cx 43 ( Cx43CT ) . ( 1 ) the c Src tyrosine kinase , which phosphorylates Cx43CT and is involved in G protein mediated inhibition of Cx 43 gap junctional communication . ( 2 ) the ZO 1 ' scaffold ' protein , which might recruit signaling proteins into Cx 43 based gap junctions . ( 3 ) microtubules ( consisting of alpha / beta tubulin dimers ) , which extend with their distal ends to Cx 43 based gap junctions , suggesting that Cx 43 gap junctions may play a novel role in regulating microtubule stability in contacted cells . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Bisphosphonate induced , hemichannel mediated , anti apoptosis through the Src / ERK pathway : a gap junction independent action of connexin 43 . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Defective activation of c Src in cystic fibrosis airway epithelial cells results in loss of tumor necrosis factor alpha induced gap junction regulation . ^^^ We have now identified the tyrosine kinase c Src as a possible pathway between the mediators of inflammation and the gap junction protein connexin 43 ( Cx 43 ) . ^^^ Finally , gap junction channel closure was prevented by expression of a Cx 43 mutant lacking tyrosine phosphorylation sites for c Src . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Characterization of PSD 95 and SAP 102 as adaptor proteins for Pyk 2 fills a critical gap in the understanding of the spatial organization of the Pyk 2 Src signaling pathway at the postsynaptic site and reveals a physiological function of the intramolecular SH 3 GK domain interaction in SAP102 . . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Conditions of ischemic insult ( e . g . ischemia or chemical hypoxia ) frequently cause the activation of protein kinases , particularly of Src and MAP kinases , and such activations are known to markedly disrupt gap junctional communication . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Our previous studies have implicated the c Src protein tyrosine kinase in mediating closure of Cx 43 based gap junctions . ^^^ Pervanadate , an inhibitor of protein tyrosine phosphatases , mimics activated Src in inhibiting Cx 43 gap junctional communication , apparently by promoting tyrosine phosphorylation of the Cx 43 C terminal tail . ^^^ However , the identity of the protein tyrosine phosphatase ( s ) that may normally prevent Src induced gap junction closure is unknown . ^^^ Our results support a model in which RPTPmu , or a closely related protein tyrosine phosphatase , interacts with the regulatory C terminal tail of Cx 43 to prevent Src mediated closure of Cx 43 gap junctional channels . . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
We previously reported that tumor necrosis factor ( TNF ) alpha decreases gap junction connectivity in cell lines derived from the airway epithelium of non cystic fibrosis ( non CF ) subjects , a mechanism that was defective in cells derived from CF patients , and identified the tyrosine kinase c Src as a possible bridge between TNF alpha and Cx 43 . ^^^ Src signaling links mediators of inflammation to Cx 43 gap junction channels in primary and transformed CFTR expressing airway cells . ^^^ These data indicate that CFTR dysfunction is associated with altered Src signaling , resulting in the persistence of gap junction connectivity in primary and transformed CF airway cells . . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Western blot analysis showed that OA induced Cx 43 Ser368 phosphorylation , and that this effect could be blocked by cotreatment with the general PKC inhibitor , calphostin C , the PKC inhibitor , eV 1 2 , or the Src kinase inhibitor , PP 1 , but not by the PKCalpha inhibitor , G6976 . eV 1 2 also prevented the OA induced disassembly of gap junctions . ^^^ Taken together , these data suggest that OA induced Cx 43 Ser368 phosphorylation is mediated by activation of PKC and Src kinase and might be responsible for OA induced gap junctional disassembly . . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Structural changes in the carboxyl terminus of the gap junction protein connexin 43 indicates signaling between binding domains for c Src and zonula occludens 1 . ^^^ In particular , c Src can disrupt the connexin 43 ( Cx 43 ) zonula occludens 1 ( ZO 1 ) interaction , leading to down regulation of gap junction intercellular communication . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Moreover , we found that endogenous c Src in normal astrocytes exists primarily in the Triton 10 100 soluble membrane fraction , distinct from the Triton insoluble fraction , which contains gap junctions . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
We demonstrate that the Src kinase can augment gap junctional communication between cells derived from homozygous null Cx 43 knockout mice . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
To investigate the role of gap junctions in cell transformsation by 5 src , we utilized a clonal cell line derived from Cx 43 knockout mice ( KoA ) that was immortalized , but not transformed . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Emphasis is on new insights into the interactions of c Src and ZO 1 with Cx 43 , interacting proteins discovered within the last 2 years ( drebrin , CIP 85 , CCN 3 ) , and feedback between gap junctions , adherens junctions ( N cadherin and catenins ) and the cytoskeleton ( microtubules and actin ) . . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Nck encodes one SH 2 and three SH 3 domains , the Src homology motifs found in nonreceptor tyrosine kinases , Ras GTPase activating protein , phosphatidylinositol 3 kinase , and phospholipase C gamma . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Presence of SH 2 domains of phospholipase C gamma 1 enhances substrate phosphorylation by increasing the affinity toward the epidermal growth factor receptor . src homology region 2 and 3 ( SH 2 and SH 3 ) domains are conserved noncatalytic regions originally described in cytoplasmic tyrosine kinases and subsequently identified in phospholipase C gamma 1 ( PLC gamma 1 ) , GTPase activating protein of ras , and other signaling proteins . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
The binding of cytoplasmic signaling proteins such as phospholipase C gamma 1 and Ras GTPase activating protein to autophosphorylated growth factor receptors is directed by their noncatalytic Src homology region 2 ( SH 2 ) domains . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Src homology 2 domains bind to tyrosine phosphorylated growth factor receptors and are found in proteins that serve as substrates for tyrosine kinases , such as phospholipase C gamma 1 and ras GTPase activating protein . ^^^ In addition to a Src homology 2 domain , GRB 7 also has a region of similarity to the noncatalytic domain of ras GTPase activating protein and is highly expressed in liver and kidney . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
It has been demonstrated that the src homology region 2 ( SH 2 domain ) of PLC gamma and of other signalling molecules such as GTPase activating protein and phosphatidylinositol 3 kinase associated p 85 direct their binding toward tyrosine autophosphorylated regions of the epidermal growth factor or platelet derived growth factor receptor . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
WT EGF receptor from EGF treated cells bound well to bacterially expressed src homology ( SH ) regions of PLC gamma 1 and to a lesser extent to bacterially expressed GTPase activating protein SH regions . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Insulin signal transduction involves the multisite docking protein insulin receptor substrate 1 ( IRS 1 ) and a number of Src homology 2 ( SH 2 ) domain factors , including p85 / p110 phosphatidylinositol 3 kinase , p 110 GTPase activating protein , and the phosphotyrosine specific phosphatase PTP1D . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
In vitro characterization of major ligands for Src homology 2 domains derived from protein tyrosine kinases , from the adaptor protein SHC and from GTPase activating protein in Ramos B cells . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Previously , we showed that SH 2 domains expressed as recombinant glutathione S transferase fusion proteins ( GST SH 2 ) from GTPase activating protein , Shc , zeta chain associated protein tyrosine kinase Zap 70 , and Src like tyrosine kinases precipitated distinct sets of phospho proteins from activated B cells . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Mitotic , tyrosine phosphorylated Sam 68 bound selectively to recombinant SH 2 domains with significantly different affinities ( c Src approximately Ras GTPase activating protein > p 85 alpha ( amino terminal ) > Grb 2 > > p 85 alpha ( COOH terminal ) ) . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
In contrast , SH 2 containing substrates , phospholipase C gamma , the GTPase activating protein of Ras , the p 85 subunit of phosphatidylinositol 3 kinase , and the Src and collagen homology protein , are not phosphorylated by the F 5 and Dc 123F mutants . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
We show that phospholipase C gamma 1 , Ras GTPase activating protein , the p 85 subunit of phosphatidylinositol 3 ' kinase , Shc , Grb 2 , Vav , Fyn , and Src are components of the Flk2 / Flt3 signal transduction pathway . ^^^ In addition , we demonstrate that phospholipase C gamma 1 , the p 85 subunit of phosphatidylinositol 3 ' kinase , Shc , Grb 2 , and Src family tyrosine kinases , but not Ras GTPase activating protein , Vav , or Nck , physically associate with the Flk2 / Flt3 cytoplasmic domain . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
By using the yeast two hybrid system , we identified p 62 , a tyrosine phosphorylated protein that associates with p21ras GTPase activating protein , as a src family kinase SH 3 domain binding protein . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Among these are dystrophin , the product of the Duchenne muscular dystrophy locus , a protein ( YAP 65 ) which associates in vitro with the Src homology domain 3 ( SH 3 ) of the Yes proto oncogene product , and a human putative GTPase activating protein . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Receptor association , as measured by coimmunoprecipitation , has been determined for phospholipase C gamma 1 , the ras GTPase activating protein , the p 85 subunit of phosphatidylinositol 3 kinase , and the src homology and collagen protein . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Mapping of sites on the Src family protein tyrosine kinases p55blk , p59fyn , and p56lyn which interact with the effector molecules phospholipase C gamma 2 , microtubule associated protein kinase , GTPase activating protein , and phosphatidylinositol 3 kinase . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
N terminal sequences contained in the Src homology 2 and 3 domains of p 120 GTPase activating protein are required for full catalytic activity toward Ras . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
This agonist stimulated PLD activity was completely blocked by the alpha 2 adrenergic receptor antagonist rauwolscine and by Clostridium botulinum toxin as well as by antibodies directed against either pp 60 ( src ) , RhoA , or Ras GTPase activating protein . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Incubation of supB+ cells with ( 13S ) HPODE and EGF increased EGFR autophosphorylation and tyrosine phosphorylation on several signaling proteins with Src homology 2 domains including GTPase activating protein . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
A potential p 120 GTPase activating protein ( RasGAP ) effector , G3BP ( RasGAP Src homology 3 [ SH 3 ] binding protein ) , was previously identified based on its ability to bind the SH 3 domain of RasGAP . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Analyses of tyrosine residue mutated PDGF receptors show that Src homology 2 domain containing proteins including Src family kinases , phosphatidylinositol 3 kinase , the GTPase activating protein of Ras , the Src homology 2 domain containing phosphatase SHP 2 , phospholipase C gamma , and Crk do not play a major role in mediating the PDGF induced activation of p 38 . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Multiple sites on Dok R were tyrosine phosphorylated following EGF stimulation ; phosphorylated Tyr 276 and Tyr 304 are proposed to dock the tandem Src homology 2 ( SH 2 ) domains of the p 21 ( Ras ) GTPase activating protein rasGAP and Tyr 351 mediates an association with the SH 2 domain of the adapter protein Nck . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
An Arf GTPase activating protein of the centaurin beta family , ASAP 1 ( also known as centaurin beta 4 ) , binds Arf and two other known regulators of the actin cytoskeleton , the tyrosine kinase Src and phosphatidylinositol 4 , 5 bisphosphate . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
The highest binding was observed with SH 3 domains of c Src , phospholipase C gamma , p 85 subunit of phosphatidylinositol 3 kinase , full length and NH ( 2 ) terminal Grb 2 , whereas binding was moderate with the SH 3 domains of amphiphysins I / II , Crk , alpha spectrin , and NADPH oxidase factor p 47 ( phox ) and negligible with the SH 3 domains of p 21 ( ras ) GTPase activating protein and COOH terminal Grb 2 . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Here we investigated the regulatory role of the Fes Src homology 2 ( SH 2 ) domain by producing a series of chimeric constructs in which the Fes SH 2 domain was replaced with those of the transforming oncogenes 5 Fps and 5 Src or by the NH 2 terminal SH 2 domain of the Ras GTPase activating protein . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
ANG 2 induced contraction of the rat LES and IAS SMC was inhibited by genistein , PD 98059 [ a specific inhibitor of MAPK kinases ( MEK 1 / 2 ) ] , herbimycin A ( a pp 60 ( c src ) inhibitor ) , and antibodies to pp 60 ( c src ) and p ( 120 ) ras GTPase activating protein ( p ( 120 ) rasGAP ) . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
ASAP 1 ( Arf GAP containing SH 3 , ankyrin repeats , and PH domain ) is a phospholipid dependent Arf GTPase activating protein ( Arf GAP ) that binds to protein tyrosine kinases Src and focal adhesion kinase . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
The CXCL12 / CXCR4 FAK pathway is membrane cholesterol dependent and impaired by metabolic inhibitors of G ( 1 ) , Src family , and the GTPase activating protein , regulator of G protein signaling 1 ( RGS 1 ) . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Insulin induced tyrosine phosphorylation of a M ( r ) 70 , 000 protein revealed by association with the Src homology 2 ( SH 2 ) and SH 3 domains of p120GAP and Grb 2 . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
It has been further established that Sam 68 is a substrate of src kinases in mitosis and that it is not associated with p120GAP in transformed fibroblasts . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
The recombinant receptor binds and phosphorylates in vitro four known cytoplasmic transducers containing src homology region 2 ( SH 2 ) domains : the 85 kDa subunit of phosphatidylinositol 3 kinase ( Pl 3 kinase ) , rasGAP , phospholipase C gamma ( PLC gamma ) , and p59Fyn , a tyrosine kinase of the src family . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Peptides based upon the 7 amino acid SH 3 binding domain core motif displayed strong binding to both the Src and to the Fyn SH 3 domains , but failed to bind to the SH 3 domain of p 21 Ras GTPase activating protein ( Ras GAP ) and other proteins . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Electroporation of anti pp60c src antibody into cultured , adherent smooth muscle cells blocked the angiotensin 2 stimulation of Ras GAP and Rho GAP tyrosine phosphorylation . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
In an attempt to identify this protein , immunoblotting was carried out with antibodies to the signal transducing proteins rasGAP , JAK 2 iNOS , p125FAK , and the Src substrate , pp 120 , but all proved negative . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
We conclude that the initial burst of Src activity is required for efficient tyrosine phosphorylation of receptor associated proteins such as PLCgamma , RasGAP , Shc , and SHP 2 and for maximal activation of Erk . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Here we identify three of these proteins as Ras GAP , the p 85 of P13 ' K and SHP 2 , while demonstrating that c Src and She were not associated with the FGFR 1 . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Thus , we examined the interactions of various SH 2 containing molecules like PLC gamma 1 , rasGAP , p 85 subunit of PI 3 kinase , Src , Fyn , Crk , Nck , Grb 2 and Shc with HEK 2 using in vitro binding assays , immunoprecipitations and yeast Two Hybrid assays . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Phosphotyrosine ( p Tyr ) dependent and independent mechanisms of p 190 RhoGAP p 120 RasGAP interaction : Tyr 1105 of p 190 , a substrate for c Src , is the sole p Tyr mediator of complex formation . p 190 RhoGAP is a 190 kDa protein that stably associates with p 120 RasGAP and regulates actin dynamics through members of the Rho family of small GTPases . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
In 32D myeloid cells and Rat 1 fibroblasts transformed by p 210 ( bcr abl ) , p 62 ( dok ) is tyrosine phosphorylated and co immunoprecipitates with Bcr Abl , RasGAP , and CrkL , a Src homology 2 ( SH 2 ) and SH 3 domain containing adaptor protein . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
RasGAP was tyrosine phosphorylated in embryos and its Src homology 2 ( SH 2 ) domains could bind in vitro to a small number of tyrosine phosphorylated proteins expressed at various developmental stages . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
SLM 1 bound the SH 2 and SH 3 domains of p 59 ( fyn ) , Grb 2 , phospholipase Cgamma 1 ( PLCgamma 1 ) , and / or p 120 ( rasGAP ) , suggesting it may function as a multifunctional adapter protein for Src during mitosis . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
The first complex , including RasGAP , p 190 RhoGAP and p 62 , was previously characterized in murine fibroblasts overexpressing c Src or transformed by 5 Src and has been correlated with cytoskeleton remodelling . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Analyses of the members of the HRG stimulated complex revealed that RAFTK is associated with p 190 RhoGAP ( p 190 ) , RasGAP and ErbB 2 , and plays an essential role in mediating the tyrosine phosphorylation of p 190 by Src . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
In addition , Src activity was blocked by the SH 2 SH3 SH 2 domains of RasGAP . ^^^ This study shows that the transduction cascade induced by the FGFR 1 FGF1 interaction in Xenopus oocytes involves RasGAP as a co activator of Src to stimulate the Ras / mitogen activated protein kinase cascade and Mos synthesis . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Further studies suggest that 5 Src phosphorylation sites on Dok 1 are critical for its binding to RasGAP and Csk , negative regulators of Src signaling . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Identification of Aurora kinases as RasGAP Src homology 3 domain binding proteins . ^^^ We looked for the existence of RasGAP Src homology 3 ( SH 3 ) domain partners as this domain is involved in the regulation of cell proliferation and has an anti apoptotic effect . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
In support of this notion , we identify several SH 2 domain containing proteins , namely c Src , NCK , and Ras GAP , that interact with caveolin 2 in a phosphorylation dependent manner . ^^^ Furthermore , our co immunoprecipitation experiments show that caveolin 2 and Ras GAP are constitutively associated in c Src expressing NIH 3T3 cells , but not in untransfected NIH 3T3 cells . . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Insulin receptor phosphorylated IRS5 / DOK4 associates with RasGAP , Crk , Src , and Fyn , but not phosphatidylinositol 3 kinase p 85 , Grb 2 , SHP 2 , Nck , or phospholipase Cgamma Src homology 2 domains , and activates MAPK in cells . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Identification of Aurora kinases as RasGAP Src homology 3 domain binding protein , also implicates these kinases as potential effectors in the Ras pathway relevant to oncogenesis . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Finally , specific signaling deficient mutant forms of the betaPDGF receptor were used to show that activation of Src , PI 3 kinase , RasGAP , PLCgamma or SHP 2 ( some of which are implicated in certain other aspects of PC 12 cell differentiation ) by RTKs is not required for growth factor induced Ca2+ channel upregulation . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
The interaction is dependent on the SH 2 ( Src homology 2 ) domains of RasGAP , which have been shown to interact with a phosphotyrosine residue within the consensus sequence ( phospho ) YXXPXD . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
These results implicate Dok 1 , Nck , Crk L , and Src kinases especially Lck , Pyk 2 , Zap 70 , Vav , and Ras GAP in intracellular signaling by SDF 1alpha / CXCL12 , and they suggest that Dok 1 plays an important role in SDF 1alpha / CXCL12 induced chemotaxis in T cells . . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Three SH 2 domain containing proteins , c Src , Nck , and Ras GAP , were found to associate with Cav 2 in a phosphorylation dependent manner . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Deletion of the binding site for PI3K , but not for Src , RasGAP , SHP 2 , or PLCgamma , completely abolished the anti apoptotic effect . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Using a panel of cytoplasmic domain mutants of the chimeric M CSF / PDGFR beta , we confirmed that the kinase domain of PDGFR beta is absolutely required for LRP tyrosine phosphorylation but that PDGFR beta mediated activation of phosphatidylinositol 3 kinase , RasGAP , SHP 2 , phospholipase C gamma , and Src are not necessary for LRP tyrosine phosphorylation . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
Upon stimulation , the K 1 ITAM was efficiently tyrosine phosphorylated and subsequently interacted with cellular Src homology 2 ( SH 2 ) containing signaling proteins Lyn , Syk , p 85 , PLCgamma 2 , RasGAP , Vav , SH 2 domain containing protein tyrosine phosphatase 1 / 2 , and Grab 2 through its phosphorylated tyrosine residues . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
E 2 did not alter beta PDGFR expression , nor did it impair the ligand induced tyrosine phosphorylation of the beta PDGFR and consecutive binding of the receptor associated signaling molecules Src homology region 2 containing phosphatase 2 , PLC gamma , phosphatidylinositol 3 kinase , and RasGAP . ^^^
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20936 and P12931	Pubmed	SVM Score :0.0
NA