| Interacting proteins: P08631 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P08631 and P20936 |
Pubmed |
SVM Score :0.0 |
| The Ras GTPase activating protein ( GAP ) is an SH 3 domain binding protein and substrate for the Src related tyrosine kinase , Hck . ^^^ In this report , we provide evidence that GAP is an SH 3 domain binding protein and substrate for the Src related tyrosine kinase Hck , which has been implicated in the regulation of myeloid cell growth , differentiation , and function . ^^^ Wild type ( WT ) or kinase inactive ( K269E ) mutant Hck proteins were co expressed with bovine GAP using the baculovirus / Sf 9 cell system . ^^^ GAP was readily phosphorylated on tyrosine by WT but not K269E Hck . ^^^ GAP was present in WT Hck immunoprecipitates from the co infected cells , indicative of Hck . ^^^ |
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| Interacting proteins: P08631 and P20936 |
Pubmed |
SVM Score :0.0 |
| We have assessed the structural impact of binding to a ligand through addition of a peptide corresponding to a proline rich region of a Hck target , the GTPase activating protein of the Ras pathway . ^^^ |
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| Interacting proteins: P08631 and P20936 |
Pubmed |
SVM Score :0.0 |
| We have fused the PPII helix region of human GAP to the C terminus of Hck SH 3 and expressed the recombinant fusion protein in Escherichia coli . ^^^ |
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