| Interacting proteins: P20936 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P20936 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P20936 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P20936 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P20936 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P20936 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P20936 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P20936 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P20936 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P20936 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P20936 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P20936 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P20936 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P20936 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P20936 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P20936 and P06239 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of GAP on tyrosine has recently been reported by several groups and may be an important step in linking signaling pathways involving p21ras and protein tyrosine kinases . p56lck , a src like protein tyrosine kinase , seems to play a crucial role in T cell development and T cell activation . ^^^ To test whether GAP is a substrate of p56lck , in vitro kinase reactions were performed with purified , recombinant GAP and p56lck . ^^^ Furthermore , coimmunoprecipitation studies provided evidence that the tyrosine phosphorylated form of GAP is bound to p56lck . ^^^ These results suggest that in T cells the function of GAP might be regulated through its phosphorylation on tyrosine and binding to the protein tyrosine kinase p56lck . . ^^^ |
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| Interacting proteins: P20936 and P06239 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylation of GAP and GAP associated proteins in lymphoid and fibroblast cells expressing lck . ^^^ In NIH3T3 fibroblasts expressing specific oncogenic and transformation defective variants of p56lck , we found that the tyrosine phosphorylation of GAP complexes required both enzymatic activation and myristylation of p56lck , and correlated with lck transforming activity . ^^^ The interaction between p 62 and p 190 from lck transformed fibroblasts and GAP could be reconstituted in vitro using bacterial TrpE fusion proteins containing GAP Src homology 2 ( SH 2 ) domains . ^^^ These results suggest that the tyrosine phosphorylation of GAP , and its interactions with SH 2 binding proteins , may be involved in fibroblast transformation by activated lck , and may participate in signal transduction and cellular transformation in lymphoid cells . . ^^^ GAP and two associated proteins , p 62 and p 190 , were shown to be phosphorylated on tyrosine in the LSTRA thymoma cell line , in which the p56lck tyrosine kinase is overexpressed as a result of retroviral promoter insertion . ^^^ |
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| Interacting proteins: P20936 and P06239 |
Pubmed |
SVM Score :0.0 |
| In addition , autophosphorylation of ZAP 70 when bound to zeta or eta resulted in the generation of multiple distinct ZAP 70 phosphorylated tyrosine residues which had the capacity to bind the SH 2 domains of fyn , lck , GAP , and abl . ^^^ |
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| Interacting proteins: P20936 and P06239 |
Pubmed |
SVM Score :0.0 |
| Recombinant GST fusion proteins containing the Hck , Src , Fyn , or Lck SH 3 domains associated with full length GAP , delta CAT , and delta SH , all of which share an N terminal proline rich region resembling an SH 3 binding motif ( PPLPPPPPQLP ) . ^^^ |
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| Interacting proteins: P20936 and P06239 |
Pubmed |
SVM Score :0.0 |
| Mapping of the p56lck mediated phosphorylation of GAP and analysis of its influence on p21ras GTPase activity in vitro . ^^^ As we showed earlier the GTPase activating protein ( GAP ) , a regulator of p21ras , is a substrate of p56lck . ^^^ Here , tryptic peptides of p56lck phosphorylated GAP were generated and analyzed by two dimensional thin layer chromatography and mass spectroscopy . ^^^ Results revealed that p56lck phosphorylates GAP specifically on Tyr 460 in vitro and in vivo . ^^^ Our results demonstrate that p56lck mediated tyrosine phosphorylation of GAP is not sufficient to change directly its effect on the intrinsic GTPase activity of p21ras . . ^^^ |
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| Interacting proteins: P20936 and P06239 |
Pubmed |
SVM Score :0.0 |
| Differential actions of p60c Src and Lck kinases on the Ras regulators p 120 GAP and GDP / GTP exchange factor CDC25Mm . ^^^ The phosphorylation of p 120 GAP by p60c Src inhibited its ability to stimulate the Ha Ras GTPase activity , whereas phosphorylation by Lck did not display any effect . ^^^ Our results suggest that phosphorylation by p60c Src and Lck is a selective process that can modulate the activity of p 120 GAP and CDC25Mm towards Ras proteins . . ^^^ |
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| Interacting proteins: P20936 and P06239 |
Pubmed |
SVM Score :0.0 |
| In contrast , Grb 2 , Crk , Abl , p 85 phosphatidylinositol 3 kinase , and GTPase activating protein SH 2 domains showed lower or no binding to Fyn or Lck SH 3 domains . ^^^ |
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| Interacting proteins: P20936 and P06239 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylated p120cbl binds to glutathione S transferase fusion proteins generated from SH 2 domains of the Fyn , Lck , and Blk protein tyrosine kinases , GTPase activating protein and phospholipase C gamma . ^^^ |
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| Interacting proteins: P20936 and P06239 |
Pubmed |
SVM Score :0.0 |
| We have shown that activation of an epidermal growth factor receptor / Lck chimera leads to the specific phosphorylation of Ras GTPase activating protein ( RasGAP ) and two RasGAP associated proteins , p 56 ( dok ) and p 62 ( dok ) . ^^^ |
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| Interacting proteins: P20936 and P06239 |
Pubmed |
SVM Score :0.0 |
| It is dependent on CD 4 expression and , in part , on the association of CD 4 with p56lck , as shown by the strongly decreased association of Sam 68 with p120GAP in the CD 4 mutants , HUT 78 CD4 , and by the reduced association of Sam 68 with both p120GAP and p56lck in the HUT 78 T cell line expressing a CD 4 mutant unable to interact with p56lck , HUT 78 C420 / 22 . ^^^ We propose that recruitment of Sam 68 , via CD4 / p56lck , to the inner face of the plasma membrane may permit , via its docking properties , the correct association of key signaling molecules including PLC gamma 1 and p120GAP . ^^^ |
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| Interacting proteins: P20936 and P06239 |
Pubmed |
SVM Score :0.0 |
| Furthermore , pp 120 was identified as rasGAP , by Western blot and immunoprecipitation experiments . rasGAP and some of its co precipitating molecules become phosphorylated in response to IL 2 , presumably by p56lck , which would thus provide a link between IL 2R and downstream events critical for NK cell proliferation and function . . ^^^ |
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| Interacting proteins: P20936 and P06239 |
Pubmed |
SVM Score :0.0 |
| The SH 2 domains of fyn , csk , phosphatidylinositol 3 ' kinase , rasGAP , vav and lck bind to pp 85 90 with varying affinities . ^^^ |
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| Interacting proteins: P20936 and P06239 |
Pubmed |
SVM Score :0.0 |
| These results implicate Dok 1 , Nck , Crk L , and Src kinases especially Lck , Pyk 2 , Zap 70 , Vav , and Ras GAP in intracellular signaling by SDF 1alpha / CXCL12 , and they suggest that Dok 1 plays an important role in SDF 1alpha / CXCL12 induced chemotaxis in T cells . . ^^^ |
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