| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.55662085 |
| To distinguish the GTPase activating effect of p 120 GAP from other effects dependent on the interaction with activated Ha Ras , the nonhydrolyzable GTP analogue guanosine 5 ' O ( thiotriphosphate ) ( GTPgammaS ) was used . 0.55662085^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.50280846 |
| Molecular cloning and nucleic acid binding properties of the GAP associated tyrosine phosphoprotein p 62 . p 62 is a tyrosine phosphoprotein that associates with p21ras GTPase activating protein ( GAP ) . 0.50280846^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.8336461 |
| The ability of p21ras to interact with GAP may be compromised by competitive binding to the product of the Ki rev 1 gene , p21rap1 . 0.8336461^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.74588751 |
| The use of ribose modified guanine nucleotides and tryptophan mutants of p21ras , neither of which have significant effect on the kinetic mechanism of the p21ras GTPase and the GAP activated p21ras GTPase , will now allow a detailed kinetic study of how GAP and other regulatory proteins interact with p21ras . 0.74588751^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.79184599 |
| The GTPase activating protein ( ras GAP ) has been shown to interact with p21ras at a domain defined by amino acids 32 40 . 0.79184599^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.59600582 |
| We report here that GAP appears to interact with p21ras at a site previously identified as the ' effector ' site , strongly implicating GAP as the biological target for regulation by p21 . . 0.59600582^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.5317927 |
| We have investigated whether complex formation between the p21ras GTPase activating protein ( GAP ) and the phosphotyrosine containing proteins p 62 and p 190 is dependent on functional p21ras , to test the hypothesis that binding of p21rasGTP to GAP enables GAP to associate with these phosphoproteins . 0.5317927^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.87192619 |
| Spectra obtained in the presence of GAP suggest that in the ground state GAP does not interact directly with the nucleotide bound to p21ras and does not induce larger conformational changes in the neighborhood of the nucleotide . 0.87192619^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.79449369 |
| This protein ( GAP ) is shown here to be ubiquitous in higher eukaryotes and to interact with H ras as well as with N ras proteins . 0.79449369^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.50964112 |
| These results indicate that although GAP , NF 1 , Raf 1 , and ralGDS all interact with H Ras in a GTP dependent manner and they are able to compete against each other for binding to H Ras , these factors share overlapping but not identical binding domains on H Ras . 0.50964112^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.71797423 |
| The three dimensional structure of the complex between human H Ras bound to guanosine diphosphate and the guanosine triphosphatase ( GTPase ) activating domain of the human GTPase activating protein p120GAP ( GAP 334 ) in the presence of aluminum fluoride was solved at a resolution of 2 . 5 angstroms . 0.71797423^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| The bovine gene for the p21ras protein activator ( RASA ) includes in its 5 ' untranslated region a ( TG ) n repeat . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Erythropoietin induces p21ras activation and p120GAP tyrosine phosphorylation in human erythroleukemia cells . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| These cells contain p21ras and p120GAP that are both functionally wild type , but barely any functional NF 1 protein . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| GAP and NF 1 can negatively regulate p21ras activity by stimulating hydrolysis of GTP bound to p21ras . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| For p21ras , two GAP proteins are known , rasGAP and the neurofibromatosis ( NF 1 ) gene product . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| This event is accompanied by the tyrosine phosphorylation of the p21ras associated GTPase activating protein p 120 ras . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| This tyrosine phosphorylation correlated with an increased association with the GTPase activating protein of p21ras ( GAP ) . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Stimulation of c fes autophosphorylation in vivo may induce interaction with GAP , resulting in altered p21ras function . . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| The excess of p21ras GTP was not due to the lack of the GTPase activating protein . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| The NF 1 tumor suppressor gene encodes neurofibromin , a GTPase activating protein ( GAP ) for p21ras ( Ras ) . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Using caged GTP the intrinsic and GAP catalyzed GTPase activity of H ras p 21 is studied . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| H ras induced inhibition of gap junction intercellular communication in rat liver epithelial cells . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| This specificity is similar to that of GTPase activating protein ( GAP ) for N ras p 21 described by M . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Platelet derived growth factor stimulation of GTPase activating protein tyrosine phosphorylation in control and c H ras expressing NIH 3T3 cells correlates with p21ras activation . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| We have sought activating substitutions in c H ras in the region encoding the effector domain , on the rationale that such mutations would dissociate effector function from negative regulation by GAP . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Correlation of increased levels of Ha ras T 24 protein with extent of loss of gap junction function in rat liver epithelial cells . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| The effects of fatty acids and phospholipids on the interaction of the full length GTPase activating protein ( GAP ) as well as its isolated C terminal domain and the Ha ras proto oncogene product p 21 were studied by various methods , viz . ^^^ The inhibition of the GTPase activating protein Ha ras interaction by acidic lipids is due to physical association of the C terminal domain of the GTPase activating protein with micellar structures . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| We found that GAP suppresses transformation of NIH 3T3 cells by normal Ha ras ( c ras ) but does not inhibit transformation by activated Ha ras ( 5 ras ) . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Infection of rat liver epithelial cells with 5 Ha ras : correlation between oncogene expression , gap junctional communication , and tumorigenicity . ^^^ The role of 5 Ha ras oncogene in tumorigenesis in an in vitro / in vivo model system was studied by investigating the expression of the Ha ras gene , gap junctional intercellular communication , and tumorigenicity as endpoints . ^^^ Gap junctional intercellular communication in 5 Ha ras infected WB cells ( WBHa ras ) , assessed by fluorescence redistribution after photobleaching ( FRAP ) , microinjection / dye transfer , and scrape loading / dye transfer techniques , was markedly decreased compared with the level in control WB cells . ^^^ The results of these studies have established a strong positive correlation between expression of the Ha ras oncogene , reduced gap junctional intercellular communication , decreased contact sensitivity , and tumorigenicity of the 5 Ha ras infected rat liver epithelial cells . . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| The C terminal 343 amino acids of GAP ( residues 702 1044 ) were observed to bind Ha ras p 21 GTP and stimulate Ha ras p 21 GTPase activity with the same efficiency ( kcat / KM congruent to 1 10 10 ( 6 ) M 1 s 1 at 24 degrees C ) as GAP purified from bovine brain or full length GAP expressed in E . coli . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Potential role of the human Ha ras oncogene in the inhibition of gap junctional intercellular communication . ^^^ We tested the effects of the expression of the human c Ha ras 1 oncogene , derived from the EJ / T4 bladder carcinoma cell line , on the ability of the Chinese hamster V 79 cells to conduct gap junctional communication . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| The HL 60 transfectants , which expressed a dominant inhibitory Ha ras Asn 17 , showed a low level of tyrosine phosphorylated GAP associated proteins and did not undergo full differentiation in response to TPA . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| The monoclonal antibody , which was found to recognize the peptide containing amino acids 275 to 351 within the amino terminal domain of GAP , did not modify the stimulation of the Ha Ras GTPase by GAP . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Besides nucleotide binding specificity , the D119N mutation has little or no effect on the interaction of Ha Ras with SDC25C , SOS 1 , GAP , or Raf . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Introduction of an oncogenic Ha ras into the GAP transfectants results in reversion to a transformed morphology and an increase in the levels of DNA methylation and DNA MeTase activity . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| The amino acid sequence shows a high degree of sequence similarity to the entire sequence of Gap1m , one of the GTPase activating proteins ( GAP ) for Ha Ras . ^^^ A recombinant protein consisting of the GAP related domain of p 98 fused to maltose binding protein stimulated GTPase activity of R Ras , and showed a weak effect on that of Ha Ras but not that of Rap 1 or Rho . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| This mutant also showed the GAP activity against Ha Ras to be similar to that of the wild type Gap 1 ( m ) . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| It was found that cells forced to express wild type Ha ras , viral Ha ras , or 5 src exhibit increased GAP activity as compared to control cells . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| The EC 50 values of Gap 1 ( m ) for Ha Ras and R Ras were 0 . 48 + / 0 . 02 and 1 . 13 + / 0 . 12 nM , respectively , whereas the EC 50 values of p120GAP for Ha Ras and R Ras were 23 . 1 + / 1 . 9 and 3 . 86 + / 0 . 38 nM , respectively . ^^^ The affinities of Gap 1 ( m ) and p120GAP to the substrates determined by competitive inhibition by using Ha Ras . ^^^ The Km values of Gap 1 ( m ) for Ha Ras and R Ras were 1 . 53 + / 0 . 27 and 3 . 38 + / 0 . 53 microM , respectively , which were lower than that of p120GAP for Ha Ras ( 145 + / 11 microM ) by almost 2 orders of magnitude . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Reduction in Ha Ras RNA was dependent on the oligoribonucleotide gap size with the minimum gap size being four nucleotides . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| In order to fill this gap the tools of molecular epidemiology are being used to study the SCC mutational spectra of p 53 and Ha ras , two of the most commonly mutated genes in human cancers . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Histochemical staining of the gap junction protein , connexin 43 , showed that psyllium restored gap junction plaques on the plasma membrane of the WB Ha ras cells . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Beta sitosterol from psyllium seed husk ( Plantago ovata Forsk ) restores gap junctional intercellular communication in Ha ras transfected rat liver cells . ^^^ We purified compounds from the husks of psyllium seeds ( Plantago ovata Forsk ; desert Indian wheat ) , beginning with an ethanol extraction then followed by HP 20 and silica gel chromatography , which restored gap junctional intercellular communication ( GJIC ) in 5 Ha ras transfected rat liver epithelial WB F 344 cell line ( WB Ha ras ) . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Taken together , our findings provide evidence for a possible role of tyrosine phosphorylation of GAP and GAP associated phosphoproteins in regulating transduction of CSF 1 induced mitogenic signals through p21ras activation . . ^^^ Activation of the colony stimulating factor 1 receptor leads to the rapid tyrosine phosphorylation of GTPase activating protein and activation of cellular p21ras . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| The p21ras GTPase activating protein ( GAP ) is thought to function as both a negative regulator and a downstream target of p21ras . ^^^ We used GAP deletion mutants that lack the domain involved in interaction with p21ras and encode essentially only the SH 2 SH3 domains . ^^^ When these GAP deletion mutants were expressed , we observed a marked induction of fos promoter activity similar to induction by activated p21ras . ^^^ Activation of the fos promoter by these GAP SH 2 SH3 regions was inhibited by cotransfection of a dominant inhibitory mutant of p21ras , Ras ( Asn 17 ) . ^^^ Thus , the induction of gene expression by GAP SH 2 SH3 domains is dependent on p21ras activity . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Requirement for Ras p 21 GTPase activating protein interaction . p21ras plays an important role in the control of cell proliferation . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Role of rap1B and p21ras GTPase activating protein in the regulation of phospholipase C gamma 1 in human platelets . ^^^ Using specific antibodies , we found that phospholipase C gamma 1 and the p21ras GTPase activating protein , rasGAP , are present in human platelets . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| However , whereas exposure of the same cells to platelet derived growth factor resulted in significant tyrosine phosphorylation of the p21ras GTPase activating protein ( GAP ) , insulin treated cells did not show any detectable levels of de novo GAP tyrosine phosphorylation . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| We investigated the involvement of the p21ras GTPase activating protein ( GAP ) in insulin induced signal transduction . ^^^ Interaction between the p21ras GTPase activating protein and the insulin receptor . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| The suggestion that p21ras GTPase activating protein ( GAP ) acts not only as a regulator of p21ras activity but also as a direct downstream target in the signaling pathway of p21ras led us to investigate the possible association of PtdIns 3 kinase with GAP . ^^^ The stimulation of Ha ras transformed epithelial cells with IGF 1 caused an increased association of PtdIns 3 kinase activity with GAP , as seen by immunoprecipitation with anti p21ras and anti GAP antibodies . ^^^ The 85 kDa regulatory subunit of PtdIns 3 kinase was present in immunoprecipitates obtained with antibodies against GAP and p21ras of IGF 1 stimulated cells . ^^^ These data suggest that GAP acts as a downstream target for p21ras via its association with PtdIns 3 kinase . . ^^^ Agonist induced association of the p21ras GTPase activating protein with phosphatidylinositol 3 kinase . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| In the GAP ras system , measurement of phosphate release is allowing the mechanism of the GAP p21ras interaction to be probed . . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Attempts to demonstrate tyrosine phosphorylation of the p21ras GTPase activating protein ( GAP ) were negative , suggesting that phosphorylation of GAP may not be the major mechanism for regulation of p21ras activity by tyrosine kinases . . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| The mechanism of GTPase activating protein ( GAP ) activation of p21ras GTP hydrolysis has been investigated by measuring the kinetics of release of Pi during the hydrolysis . ^^^ This provides a non radioactive method of determining p21ras concentration and GAP activity . ^^^ It was used to determine the interaction of GAP with wild type p21ras and two mutants ( Leu 61 / Ser 186 and Asp 12 ) , all in the GTP ( or guanosine 5 ' [ beta gamma imido ] triphosphate ) form . ^^^ Interaction of GTPase activating protein with p21ras , measured using a continuous assay for inorganic phosphate release . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Association of a tyrosine kinase activity with GAP complexes in 5 src transformed fibroblasts . p21ras GAP is phosphorylated on tyrosine residues and associates with 62 kDa and 190 kDa tyrosine phosphorylated proteins in 5 src transformed fibroblasts . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Ras GTPase activating protein ( GAP ) is a cytoplasmic factor that regulates the GTPase activity of p21ras . ^^^ Phosphorylation of GAP on tyrosine has recently been reported by several groups and may be an important step in linking signaling pathways involving p21ras and protein tyrosine kinases . p56lck , a src like protein tyrosine kinase , seems to play a crucial role in T cell development and T cell activation . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Introduction of the catalytic region of GAP into this line resulted in morphological reversion and lower in vivo GTP binding by endogenous p21ras . ^^^ The tumor lines expressed normal levels of p120GAP and p21ras . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| The neurofibromatosis 1 ( NF 1 ) gene product , neurofibromin , contains a GTPase activating protein ( GAP ) related domain , or NF 1 GRD , that is able to down regulate p21ras by stimulating its intrinsic GTPase . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| We report here that p21ras GTPase activating protein ( rasGAP ) or rasGAP associated proteins p 190 and p 62 are phosphorylated on tyrosine in Ph 1 ( + ) cell lines . ^^^ These results suggest that rasGAP or associated proteins are potential substrates for p210BCR / ABL kinase and thus directly link p210BCR / ABL with a signal transduction pathway known to be activated by hematopoietic growth factors ( p21ras ) . . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| This raises the possibility that p 190 , acting via GAP , can transduce signals from p21ras to the nucleus , perhaps affecting expression of specific cellular genes . . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Several proteins that are involved in the control of the activity of p21ras have now been characterised . p120GAP stimulates the GTPase activity of p21ras and hence acts as a negative regulator of ras proteins . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| The intrinsic GTPase activity of the cellular protein p21ras is strongly increased by two cytosolic proteins , the GTPase activating protein ( GAP ) produced by the neurofibromatosis type 1 gene ( NF 1 GAP ) and a GAP of 120 kDa molecular mass ( p 120 GAP ) . ^^^ The GAP mediated stimulation of p21ras GTPase activity was measured in cytosol obtained from carcinogen induced liver tumors and normal liver tissues of mice of two strains , namely C3H / He and C57BL / 6J . ^^^ Since both GAP proteins are important cellular regulators of the ras signaling pathway and probably also effectors of p21ras , the observed differences in GAP activity may be of relevance for the tumorigenic process in mouse liver . . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| There is strong , albeit indirect , evidence for a mitogenic signal transduction pathway comprising growth factors , growth factor receptors , the GTPase activating protein ( p 120 GAP ) , and p21ras . ^^^ Phosphorylated p 120 GAP is as active in stimulating the p21ras . ^^^ GTPase as unphosphorylated GAP . p 120 GAP , however , when bound to the EGF receptor is by a factor of 2 less active in stimulating the p21ras . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Analysis of biochemical pathways implicated in signal transduction by growth factor receptors indicated that both phospholipase C type gamma ( PLC gamma ) and the p21ras GTPase activating protein ( GAP ) are substrates for the erbB 2 kinase in NIH 3T3 fibroblasts . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| The regulatory effect of protein kinase C on p21ras GTPase activity appears to be mediated via regulation of GAP , the GTPase activating protein of p21ras . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| The presence of two adjacent SH 2 domains in the p21ras GTPase activating protein ( GAP ) indicates that GAP might interact directly with tyrosine kinases . ^^^ Our results suggest a mechanism by which tyrosine kinases might modify p21ras function , and implicate GAP and its associated proteins as targets of both oncoproteins and normal growth factor receptors with tyrosine kinase activity . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| GTPase activating protein ( GAP ) is a cytosolic protein that stimulates the rate of hydrolysis of GTP ( GTP to GDP ) bound to normal p21ras , but does not catalyze the hydrolysis of GTP bound to oncogenic , activated forms of the ras protein . ^^^ From these data , we suggest that tyrosine phosphorylation and stable association of p 64 with GAP is an important step in mediating cellular signaling through the p21ras GAP pathway . . ^^^ GTPase activating protein ( GAP ) is a cytosolic protein that stimulates the rate of hydrolysis of GTP ( GTP to GDP ) bound to normal p21ras , but does not catalyze the hydrolysis of GTP bound to oncogenic , activated forms of the ras protein . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Purification of a plasma membrane associated GTPase activating protein specific for rap1 / Krev 1 from HL 60 cells . rap1 / Krev 1 is a p21ras related GTP binding protein that has been implicated in the reversion of the ras transformed cell phenotype . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| GTPase activating protein ( GAP ) stimulates the ability of p21ras to hydrolyze GTP to GDP . ^^^ GTPase activating protein ( GAP ) stimulates the ability of p21ras to hydrolyze GTP to GDP . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Ras GAP ( GTPase activating protein ) is a regulatory protein that stimulates the intrinsic guanosine triphosphatase ( GTPase ) activity of the proto oncogene product p21ras . ^^^ A domain of the neurofibromatosis gene product ( NF 1 ) that has sequence similarity to the catalytic domain of Ras GAP and to yeast IRA gene products also has a specific stimulatory activity toward p21ras GTPase . ^^^ Arachidonic acid and phosphatidic acid inactivate GAP , but no agents have been identified that stimulate GAP and thereby switch p21ras off . ^^^ Ras GAP ( GTPase activating protein ) is a regulatory protein that stimulates the intrinsic guanosine triphosphatase ( GTPase ) activity of the proto oncogene product p21ras . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| NF 1 GAP binds to the p21ras proteins up to 300 times more efficiently than p 120 GAP . ^^^ This inhibition does not compete with p21ras , and lipid inactivated NF 1 GAP can still bind p21ras . ^^^ These results indicate that more than one GAP regulates p21ras in the same cell . . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Amino acid sequence homology between the GTPase Activating Protein ( GAP ) and the GTP binding regulatory protein , Gs alpha , suggests that a specific region of GAP primary structure ( residues 891 898 ) may be involved in its stimulation of p21ras GTP hydrolytic activity ( McCormick , F . [ 1989 ] Nature 340 , 678 679 ) . ^^^ A peptide , designated p 891 , corresponding to GAP residues 891 906 ( M891RTRVVSGFVFLRLIC906 ) was synthesized and tested for its ability to inhibit GAP stimulated p21ras GTPase activity . ^^^ Thus , the inhibitory effect of p 891 on GAP stimulation of p21ras suggests that amino acids within the region 891 906 of GAP may be essential for interaction with p21ras . ^^^ A synthetic peptide corresponding to a sequence in the GTPase activating protein inhibits p21ras stimulation and promotes guanine nucleotide exchange . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| The p21ras GTPase activating protein ( GAP ) down regulates p21ras by stimulating its intrinsic GTPase activity . ^^^ These results indicate that protein tyrosine kinases induce GAP to form multiple heteromeric complexes , which are strong candidates for regulators or targets of p21ras . . ^^^ Protein tyrosine kinases regulate the phosphorylation , protein interactions , subcellular distribution , and activity of p21ras GTPase activating protein . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| A GTPase activating protein ( GAP ) mediates the inactivation of p21ras by facilitating the conversion of the active p21ras GTP to the inactive p21ras GDP . ^^^ This predicts that overexpression of GAP would inactivate p21ras and block transformation of cells by src . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Two proteins , GAP and the tumor suppressor NF 1 , interact with p21ras in this region but it is an unresolved puzzle whether either of these is the an unresolved puzzle whether either of these is the effector . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| We therefore analyzed the interaction between the c rasH ( S39C ) protein and the potential target molecules GTPase activating protein ( GAP ) and the GAP related domain of NF 1 , on the basis of stimulation of the mutant p21ras GTPase activity by these molecules in vitro . ^^^ We conclude that for this mutant , there is a dissociation between the stimulation of p21ras GTPase activity by GAP and the GAP related domain NF 1 and their potential target function . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Phospholipase C gamma 1 ( PLC gamma 1 ) and p21ras guanosine triphosphatase ( GTPase ) activating protein ( GAP ) bind to and are phosphorylated by activated growth factor receptors . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Cytoplasmic proteins that regulate signal transduction or induce cellular transformation , including cytoplasmic protein tyrosine kinases , p21ras GTPase activating protein ( GAP ) , phospholipase C gamma , and the 5 crk oncoprotein , possess one or two copies of a conserved noncatalytic domain , Src homology region 2 ( SH 2 ) . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Although GAP is located predominantly in the cytosol , most tyrosine phosphorylated GAP is associated with the cell membrane , the site of p21ras biological activity . ^^^ These results provide a direct biochemical link between activated PDGF receptor tyrosine kinases and the p21ras GAP mitogenic signalling system . . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| It has been shown that this GTPase activating protein , or GAP , interacts with the effector domain of ras , leading us and others to propose that GAP may be the target for regulation by p21ras . ^^^ It appears that p23R ras interacts with the same 125 kDa GAP protein as p21ras whereas p21rho interacts with a distinct 29 kDa protein , rho GAP . . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Upon sedimentation of detergent extracts from crosslinked cells on sucrose gradients , a p 21 p60 complex could be demonstrated with a Mr of 200 , 000 300 , 000 , p 60 does not appear to be related to pp60src nor to the cytosolic GTPase activating protein that interacts with p21ras to enhance its GTPase activity . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| We propose that this domain constitutes an intrinsic activator of GTP hydrolysis , and that it performs a function analogous to that performed for EF Tu by the programmed ribosome and for p21ras by the recently discovered GTPase activating protein . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| This latent inhibitory activity of 5 fps SH 2 has functional implications for phospholipase C gamma and p21ras GTPase activating protein , both of which have two distinct SH 2 domains suggestive of complex regulation . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Aberrant expression of p21RAS but not p120GAP is a common feature of myelodysplasia . ^^^ We have examined the expression of p21RAS and its negative regulator / downstream effector , p120GAP , in combination with lineage specific cluster of differentiation markers in normal and preleukaemic myeloid bone marrow cells using flow cytometry . ^^^ The results of the study suggest that over expression of the RAS gene product , p21RAS , may represent an alternative or additional mechanism of activation of the RAS signalling pathway and that this may play a role in leukaemogenesis , however , there is no evidence from this study that loss of p120GAP expression is a feature of myelodysplasia . . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Kinetics of inorganic phosphate release during the interaction of p21ras with the GTPase activating proteins , p 120 GAP and neurofibromin . ^^^ The rate of GTP hydrolysis on p21ras is accelerated by approximately 10 ( 5 ) times by the catalytic domains of GTPase activating proteins ( GAPs ) , p 120 GAP ( GAP 344 ) or neurofibromin ( NF 1 334 ) . ^^^ Measurements were made in real time with a stopped flow apparatus , in which the p21ras complex with the 2 ' , 3 ' methanthraniloyl analogue of GTP ( mantGTP ) was mixed with the GAP in the presence of this Pi probe . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| In addition , this cell fraction contained the tyrosine kinases pp60c src and pp62c yes and the p21ras GTPase activating protein ( GAP ) . ^^^ Association of GP IIb IIIa , pp60c src , pp62c yes , and the p21ras GTPase activating protein with the membrane skeleton . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| A protein that is highly related to GTPase activating protein associated p 62 complexes with phospholipase C gamma . p 62 is a highly tyrosyl phosphorylated protein that was first identified in immunoprecipitates of the GTPase activating protein ( GAP ) of p21ras from cells transformed by oncogenic nonreceptor tyrosine kinases or stimulated through tyrosine kinase receptors ( C . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Several tyrosine phosphorylated proteins have been identified that associate with p120GAP , the GTPase activating protein of p21ras . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| PDGF action triggers both mechanisms of Grb 2 disassembly , which probably operate in concert with GAP to attenuate p21ras signaling . . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Although neurofibromin is a GTPase activating protein ( GAP ) for p21ras proteins , its loss in the BXH 2 leukemic cell lines was not correlated with an increased steady state level of p21ras bound to GTP . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| GTPase activating protein ( GAP ) , a protein capable of regulating the activity of p21ras protein , is phosphorylated on tyrosine residues following the activation of tyrosine kinase ( s ) associated with several growth factor receptors . ^^^ GTPase activating protein ( GAP ) , a protein capable of regulating the activity of p21ras protein , is phosphorylated on tyrosine residues following the activation of tyrosine kinase ( s ) associated with several growth factor receptors . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Increased levels of p21ras GTP and enhanced DNA synthesis accompany elevated tyrosyl phosphorylation of GAP associated proteins , p 190 and p 62 , in c src overexpressors . ^^^ Here we identify two of these proteins as the GAP ( GTPase activating protein of p21ras ) associated proteins , p 190 and p 62 . ^^^ Taken together , these results suggest that one mechanism by which pp60c src may contribute to early events in the EGF induced mitogenic pathway in 10T1 / 2 fibroblasts is by increasing the level of GAP associated p 190 and p 62 tyrosyl phosphorylation , which in turn results in higher levels of p21ras GTP . . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Here , we show that a component of pps 120 , p 125 , was specifically immunoprecipitated with antibodies against the p21ras GTPase activating protein ( GAP ) . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Finally , we also show that a 62 kD protein coimmunoprecipitating with the p21ras GTPase activating protein ( GAP ) is heavily tyrosine phosphorylated only after CD 2 stimulation . ^^^ They also suggest a function of the p 62 GAP associated protein as a link between PLC gamma 1 and p21ras activation pathways after CD 2 activation . . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Two of the interacting tyrosine phosphorylated proteins were identified as the p 85 subunit of phosphatidylinositol 3 kinase and the GTPase activating protein of p21ras . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Guanosine triphosphatase activating protein ( GAP ) is an important modulator of p21ras ( Ras ) dependent signal transduction in mammalian cells and in insulin induced maturation of Xenopus oocytes . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| By using the yeast two hybrid system , we identified p 62 , a tyrosine phosphorylated protein that associates with p21ras GTPase activating protein , as a src family kinase SH 3 domain binding protein . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| In the present studies , insulin was found to stimulate in a rat hepatoma cell line ( called FAO cells ) the tyrosine phosphorylation of the 60 kilodalton p21ras GTPase activating protein ( GAP ) associated protein called p 60 . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Stimulation with platelet derived growth factor ( PDGF ) results in the association of several SH 2 domain containing proteins with the activated PDGF receptor , including GAP , a GTPase activating protein of p21ras , and phosphatidylinositol 3 kinase ( PI 3K ) . ^^^ To investigate the role of GAP PI 3K receptor interaction in p21ras signaling , we have used cell lines expressing mutant PDGF receptors that either are impaired in GAP binding or fail to bind both GAP and PI 3K . ^^^ These results indicate that binding of GAP and / or PI 3K to the PDGF receptor is not necessary for PDGF induced p21ras activation and p21ras mediated signaling to ERK 2 . ^^^ We also show that , in contrast to the activation of ERK 2 , PDGF induced GAP and PI 3K interaction with the PDGF receptor are not inhibited by p21ras ( asn 17 ) expression , indicating that these interactions do not require p21ras activation . . ^^^ Platelet derived growth factor induced p21ras mediated signaling is independent of platelet derived growth factor receptor interaction with GTPase activating protein or phosphatidylinositol 3 kinase . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Grb 2 , Sos , Ras Gap , p21ras , Raf , MEK and MAPK , genetic studies have suggested that two novel proteins , the protein tyrosine phosphatase ( PTPase ) Csw and the transmembrane protein Rho , are involved in RPTK signalling . . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Mapping of the p56lck mediated phosphorylation of GAP and analysis of its influence on p21ras GTPase activity in vitro . ^^^ As we showed earlier the GTPase activating protein ( GAP ) , a regulator of p21ras , is a substrate of p56lck . ^^^ The effect of tyrosine phosphorylation of GAP on its GTPase activating activity versus p21ras was then tested using a p21ras dependent GTPase assay system . ^^^ Our results demonstrate that p56lck mediated tyrosine phosphorylation of GAP is not sufficient to change directly its effect on the intrinsic GTPase activity of p21ras . . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| The p210bcr / abl tyrosine kinase appears to be responsible for initiating and maintaining the leukemic phenotype in chronic myelogenous leukemia ( CML ) patients . p21ras p120GAP interactions play a central role in transducing mitogenic signals . ^^^ Therefore , we investigated whether p21ras and p120GAP are regulated by p210bcr / abl , and whether this activation is functionally significant for CML cell proliferation . ^^^ We report that transient expression of p210bcr / abl in fibroblast like cells induces simultaneous activation of p21ras and inhibition of GTPase promoting activity of p120GAP , and confirm these data showing that downregulation of p210bcr / abl expression in CML cells with bcr / abl antisense oligodeoxynucleotides induces both inhibition of p21ras activation and stimulation of GTPase promoting activity of p120GAP . ^^^ Thus , the p210bcr / abl dependent regulation of p120GAP activity is responsible , in part , for the maintenance of p21ras in the active GTP bound form , a crucial requirement for CML cell proliferation . . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| There is evidence to suggest that the p 120 GAP ( GAP ) , originally described as an inhibitor of p21ras , may also serve as a downstream effector of ras regulated signal transduction . ^^^ Stimulation of MO 7 cells with hematopoietic growth factors increased the expression of GAP as well as the levels of active GTP bound p21ras . ^^^ These data indicate that p 120 GAP is involved in human normal and leukemic hemopoiesis and strongly suggest that GAP is not only a p21ras inhibitor ( signal terminator ) , but also a positive signal transducer . . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Among the 110 130 kDa group of phosphotyrosyl proteins is the 125 kDa `` focal adhesion kinase ' ' ( p125FAK ) but not the 120 kDa p21ras GTPase activating protein . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| In contrast , tyrosine phosphorylation of previously described substrates of the PDGF receptor tyrosine kinase , namely the p21ras GTPase activating protein , p 120 , phosphatidyl inositol 3 ' kinase , and phospholipase C gamma exhibited sigmoidal dose response curves with PDGF and were all efficiently phosphorylated on tyrosine at 30 ng / ml PDGF . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| We studied the tyrosine phosphorylated proteins which might be involved in the signaling pathway p185BCR ABL using a Ph positive acute lymphoblastic leukemia cell line . p185BCR ABL but not p145c abl was constitutively phosphorylated on tyrosine in this cell line . p21ras GTPase activating protein ( GAP ) was physically associated with p185BCR ABL , but not with p145c abl , and GAP associated proteins p62 / p190 were found to be tyrosine phosphorylated . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| The PDGF receptor alpha subunit activates p21ras and triggers DNA synthesis without interacting with rasGAP . ^^^ Despite its apparent inability to interact with rasGAP , the alpha subunit was fully able to trigger PDGF dependent p21ras activation and DNA synthesis . ^^^ We conclude that the PDGFR alpha subunit does not mediate tyrosine phosphorylation or associate with ras GAP , and that these events are not required for PDGF AA mediated activation of p21ras or DNA synthesis . . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| The intrinsic GTPase activity of cellular protein p21ras is strongly increased by cytosolic GTPase activating protein ( GAP ) . ^^^ These results suggest that production of DG in response to hormones or growth factors stimulation could indirectly modulate the interaction between p21ras and GAP . . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| These oncogenes , which are frequently expressed in human malignancies , code for proteins ( p21ras ) that are locked in the activated GTP bound state because their GTPase is refractory to the ras specific GTPase activating protein ( GAP ) . ^^^ The protein encoded by NF 1 contains a GAP homology region , binds p21ras GTP , and stimulates the hydrolysis of p21ras bound GTP . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| The intrinsic GTPase activity of cellular protein p21ras is strongly increased by cytosolic GTPase activating protein ( GAP ) . ^^^ These results suggest that production of DG in response to hormones or growth factors stimulation could indirectly modulate the interaction between p21ras and GAP . . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Targets of B lymphocyte antigen receptor signal transduction include the p21ras GTPase activating protein ( GAP ) and two GAP associated proteins . ^^^ We now show that the p21ras GTPase activating protein ( GAP ) is also a substrate for mIg activated tyrosine kinases . p21ras is a key regulator of cell growth and GAP may act as both a regulator of p21ras activity and as a downstream effector of p21ras . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Although p21ras is localized to the plasma membrane , proteins it interacts with , such as the GTPase activating proteins ( GAPs ) ras GAP and neurofibromin ( NF 1 ) , are not , suggesting that one function of p21ras GTP may be to target such proteins to the plasma membrane . ^^^ To investigate the effects of targeting ras GAP to the plasma membrane , ras C terminal motifs sufficient for plasma membrane localization of p21ras were cloned onto the C terminus of ras GAP . ^^^ However , if ras GAP is involved in the effector functions of p21ras , it can only be part of the effector complex for cell transformation . . ^^^ Plasma membrane targeted ras GTPase activating protein is a potent suppressor of p21ras function . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Structural requirements for the interaction of p21ras with GAP , exchange factors , and its biological effector target . ^^^ These two regions change conformation on GTP binding by p21ras and , accordingly , both GAP binding and Ras biological activity are GTP dependent processes . ^^^ In addition , certain mutations in the switch 1 and 2 regions alter the affinity of GAP for p21ras , again implicating this region in the binding interaction . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Essential role of Arg 903 of GAP in activation of GTP hydrolysis on p21ras . ^^^ In order to address this question , we have developed a simple physical method to study formation of GAP . p21ras complexes . ^^^ This utilizes the increase of fluorescence anisotropy upon binding of GAP to p21ras complexed with 2 ' ( 3 ' ) O ( N methylanthraniloyl ) ( mant ) derivatives of guanine nucleotides . ^^^ Dissociation constants obtained for the catalytic domains of either p 120 GAP ( GAP 344 ) or neurofibromin ( NF 1 GRD ) with normal and Leu 61 p21ras proteins are comparable with those obtained by kinetic methods . ^^^ The fluorescence anisotropy method allowed us to show that mutation of Arg 903 , within the FLR motif of GAP , can result in protein defective in catalysis but not in binding to p21ras . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| The Ras GTPase activating protein ( RasGAP ) is an important modulator of p21ras dependent signal transduction in Xenopus oocytes and in mammalian cells . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Furthermore , removal of the inhibitory influence of PI 3 kinase on GAP resulted in dose dependent decreases in the ability of insulin to stimulate p21ras . ^^^ Inhibition of PI 3 kinase activity or immunodepletion of either one of its subunits results in activation of GAP and decreases in GTP loading of p21ras . . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| We also analyzed the stimulated GTPase reaction of p21ras by the GTPase activating protein ( GAP ) and the GTPase reaction of Rap1A , a Ras related GTP binding protein , with similar approaches . ^^^ Linear free energy relationships in the intrinsic and GTPase activating protein stimulated guanosine 5 ' triphosphate hydrolysis of p21ras . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| On the other hand , it was found that the corresponding Brnsted slope for the GTPase reaction of p21ras in the presence of GTPase Activating Protein ( GAP ) is about beta = 4 . 9 . ^^^ Finally , we analyze the observed LFER for the GTPase reaction of p21ras in the presence of GAP and discuss its relevance for the mechanism of GAP activation . . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Analysis of specific effector molecules revealed that tyrosine phosphorylation of Shc , but not rasGAP or Vav , correlated with the unique ability of BCR ligation to trigger p21ras activation in CD45+ cells . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| The activity of GAP in increasing the intrinsic GTPase activity of p21RAS was found to be much less in NDEA treated rats as compared to that in control rats . ^^^ It is speculated that phosphorylated GAP helps keep the p21RAS in the more active GTP bound state . . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| It is shown here for p21ras , a well studied example of GTP hydrolysing proteins , that the GTP hydrolysis rate is significantly faster if Mg2+ is replaced by Mn2+ , both in the presence or absence of its GTPase activating protein Ras GAP . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| These changes alter the conformation of the protein resulting in insensitivity of the protein to the GTPase activating protein which normally hydrolyses the active p21RAS GTP bound form to the inactive GDP bound form . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Sam 68 association with p120GAP in CD4+ T cells is dependent on CD 4 molecule expression . p 120 GTPase activating protein ( p120GAP ) is a major negative regulator of p21ras activity in several cell types including T cells . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| In addition , sulindac sulfide can impair the nucleotide exchange on p21ras by CDC 25 as well as the acceleration of the p21ras GTPase reaction by p120GAP . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| We determined the expression of p21ras , and its regulatory elements p 120 ras GAP and hSOS , in PBMC of 10 patients with inactive SLE ( mean SLEDAI score 1 . 8+ / 0 . 53 ) and 10 age and sex matched healthy controls . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Expression of p21ras and its regulatory elements , the GTPase activating protein p120ras GAP and the guanine nucleotide releasing factor ( GNRF ) hSOS , was comparable in the three groups . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| In three of four tumors examined with activated wild type p21ras , we observed increased c erbB 2 receptor expression and decreased Ras GAP expression . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Structural changes induced in p21Ras upon GAP 334 complexation as probed by ESEEM spectroscopy and molecular dynamics simulation . ^^^ BACKGROUND : The means by which the protein GAP accelerates GTP hydrolysis , and thereby downregulates growth signaling by p21Ras , is of considerable interest , particularly inasmuch as p 21 mutants are implicated in a number of human cancers . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Gap junction intercellular communication ( GJIC ) , H ras oncogene expression and Ras oncogene product ( P21ras protein ) expression were studied in four human solid tumor cell lines , W 1 38 , CACO 2 , A 549 and PaCa ( with the different Ras gene mutation rate ) , and the effects of four compounds , Salvia miltiorrhiza derivative ( SMD ) , d limonene , turmeric derivative 1 ( TD 1 ) and turmeric derivative 2 ( TD 2 ) , on them . ^^^ |
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| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Levels of p21Ras stimulatory element hSOS 1 but not p21Ras and its inhibitory element p120GAP were significantly decreased in SLE patients . ^^^ Early p21Ras signalling was down regulated in SLE patients with active disease as indicated by the decreased membrane / cytoplasmic ( M / C ) ratios of the p21Ras regulatory elements hSOS 1 and p120GAP and by the non responsiveness of these ratios to cellular stimulation . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| The expression of p21Ras and its regulatory proteins ; hSOS 1 and p120GAP were studied . ^^^ Expression of p21Ras , and its regulatory proteins hSOS 1 and p120GAP were similar in PCOD patients and controls . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Untreated SLE afflicted mice demonstrated increased expression of p21Ras and the phosphorylated active form of its down stream element JNK kinase in conjunction with reduced hSOS and unchanged p120GAP , as compared to healthy controls . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Neurofibromatosis Type 1 ( NF 1 ) is a common neurological disorder caused by mutations in the gene encoding Neurofibromin , a p21Ras GTPase Activating Protein ( GAP ) . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Weakly transforming mutations that either reduce intrinsic and GTPase activating protein ( GAP ) stimulated GTPase activities ( 61P ) or enhance guanine nucleotide exchange rates ( 116H , 119E ) were combined into the same H ras proteins . ^^^ Although both 61P and 61L mutations result in reduced intrinsic GTPase activity and loss of GAP stimulation in vitro , only H ras ( 61L ) exhibits strong transforming activity . ^^^ |
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| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| From porcine liver we identified and partially purified a GTPase activating protein ( yptGAP ) that is similarly active with mouse ypt1p / rab1p and yeast Ypt1p but is inactive with H ras protein as a substrate . ^^^ |
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| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| The G60A mutation also exerts little effect on the interaction of H ras with SDC25C ( a guanine nucleotide exchange factor ) and GAP . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| The gene , GYP 6 ( GAP of Ypt 6 protein ) , encodes a protein of 458 amino acids which is highly specific for the Ypt 6 protein and shows little or no cross reactivity with other Ypt / Rab family members or with H Ras p21 . . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| It was possible to construct Ras2p ' s resistant to Ira2p but still sensitive to human p 120 GAP and , conversely , a H ras p 21 sensitive to Ira2p . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| R Ras is regulated by the same GAP molecules as H Ras and the other Ras protooncogene products , and may therefore be activated in a manner co ordinate with these growth promoting proteins . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| By using methods based on inhibition of yeast adenylyl cyclase or RasGAP activity and by in vitro binding assays , we have shown that the segment of B Raf corresponding to amino acid 1 326 binds directly to H Ras with a dissociation constant ( Kd ) comparable to that of Raf 1 and that the binding is not significantly affected by the post translational modifications . ^^^ |
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| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| When tested for RasGAP activity , recombinant DGAP 1 protein did not promote the GTPase activity of human H Ras or of Dictyostelium RasG in vitro . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Like its mammalian homolog , Drosophila RasGAP stimulated the intrinsic GTPase activity of normal mammalian H Ras but not that of the oncogenic Val 12 mutant . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| All papillomas analyzed ( six of six ) had mutations in codon 61 of H ras , demonstrating strong cooperation between the Nf 1 GTPase activating protein for Ras , neurofibromin , and Ras GTP . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Inhibition of cyclooxygenase 2 expression and restoration of gap junction intercellular communication in H ras transformed rat liver epithelial cells by caffeic acid phenethyl ester . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Finally , we demonstrated that annexin A 6 promotes plasma membrane targeting of p120GAP in A 431 cells in response to a variety of stimuli , resulting in colocalization with H Ras . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Role of protein kinase C in the deficient gap junctional intercellular communication of K ras transformed murine lung epithelial cells . ^^^ We investigated the roles of Ras and PKC in the deficient gap junctional intercellular communication ( GJIC ) of K ras transformed E 9 mouse lung carcinoma cells . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| With regards to the codon 15 , 16 , 18 and 31 mutant K Ras proteins ( p21BM2 ) , the GTPase activity in the presence of GAP is much lower than that of the normal K Ras protein , whereas the intrinsic GTPase activity is nearly the same as that of the normal K Ras protein . ^^^ The purified K Ras protein from E . coli were then measured for their intrinsic GTPase activity and the GTPase activity in the presence of GTPase activating protein for Ras . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| We found that the extrinsic GTPase activity of the codon 15 mutant K ras proteins ( p 21 ( K ras15M ) ) in the presence of GAP is much lower than that of the wild type K ras protein ( p 21 BN ) , whereas the intrinsic GTPase activity is nearly the same as that of the wild type K ras protein . ^^^ The purified K ras protein from E . coli was then measured for its intrinsic GTPase activity and the extrinsic GTPase activity in the presence of GTPase activating protein for ras . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Collectively , our results show that K RAS is the primary target for neurofibromin GTPase activating protein activity in vitro and in vivo and that K RAS activation in astrocytes recapitulates the biochemical , biological , and tumorigenic properties of neurofibromin loss . . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| The effectiveness of Rap1A to inhibit Ras GAP is dependent upon the amount of Ras GAP present in the assay and can also be overcome by the addition of GTP bound N Ras ( GC 43 ) , suggesting a competitive mechanism is operative . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| A fragment of NF 1 cDNA encoding the GAP related domain ( NF 1 GRD ) was expressed , immunoaffinity purified , and assayed for effects on N ras p 21 GTPase activity . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| Human ras GTPase activating protein ( GAP ) is a cytoplasmic factor that stimulates the GTPase activity of normal N ras p 21 while having no stimulatory effect on the GTPase activity of oncogenic variants of N ras p 21 . ^^^ |
|
| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| We investigated the mutational status of the N ras gene and the FLR exon of codons 1371 1423 of the open reading frame of the full length NF 1 cDNA , which has a strong homology with the mammalian ras GTPase activating protein ( GAP ) , especially for a stretch of three consecutive amino acids ( F , L , R ) , by single strand conformation polymorphism analysis and direct sequencing in samples from patients with AML . ^^^ |
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| Interacting proteins: P01112 and P20936 |
Pubmed |
SVM Score :0.0 |
| The ras gene family ( H , K and N ras ) encodes the Ras protein , a GTPase activating protein that regulates several signal transduction pathways including cellular proliferation and differentiation . ^^^ |
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