| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| The expression of the myelin PLP , BP , and MAG genes was determined by Northern blot analysis . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| These include mainly myelin components ( PLP , MAG , and MOG ) , but also , the astroglial S 100 beta protein . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| These include MBP , PLP , MOG , MAG , CNP and S 100 . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Oligodendrocytes contain abnormal vacuoles and stain intensely for PLP and other proteins such as MAG . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Eight of these 17 genes encode structural components of myelin ( CNP , MAG , MAL , MOG , MOBP , PMP 22 , PLLP , PLP 1 ) . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| One oligodendrocyte like clone ( DM 5 ) that we have succeeded in maintaining at 33 degrees C can be induced to express galactocerebroside and 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase at the elevated temperature but never expresses the more differentiated markers of the myelination state , such as the myelin proteolipid protein or the myelin associated glycoprotein . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Numbers of IL 2 secreting cells were higher in MS patients compared to patients with AM + TH after stimulation with myelin proteolipid protein ( PLP ) , myelin associated glycoprotein ( MAG ) and myelin oligodendrocyte glycoprotein ( MOG ) , but not after stimulation with myelin basic protein ( MBP ) . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Likewise , concentrations of myelin specific proteins ( MBP , myelin proteolipid protein , MAG , and 2 ' , 3 ' cyclic nucleotide , 3 ' phosphodiesterase ) were not significantly different in Igf 1 ( / ) and WT mice . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| The priming infectious agent must show molecular mimicry with self CNS antigens such as glial fibrillary acidic protein ( GFAP ) , myelin associated glycoprotein ( MAG ) or myelin proteolipid protein ( PLP ) . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Myelin proteolipid protein , basic protein , the small isoform of myelin associated glycoprotein , and p42MAPK are associated in the Triton 10 100 extract of central nervous system myelin . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| METHODS : The expression of cyclic nucleotide phosphodiesterase , glial fibrillary acidic protein , myelin associated glycoprotein , myelin basic protein , and myelin proteolipid protein were assessed in the superior frontal gyrus and the primary motor cortex of control , uncomplicated alcoholic , and cirrhotic alcoholic cases . ^^^ RESULTS : Overall , the expression of cyclic nucleotide phosphodiesterase , glial fibrillary acidic protein , myelin associated glycoprotein , and myelin basic protein were significantly lower in the cirrhotic alcoholic cases compared with controls , with a similar tendency for myelin proteolipid protein . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Differential upregulation of PLP and MAG genes in C 6 glioma cells by N2A neuroblastoma conditioned medium . ^^^ The effect of factors released from N2A neuroblastoma cells on the expression of myelin protein genes in glioma C 6 cells , i . e . , proteolipid protein ( PLP ) and myelin associated glycoprotein ( MAG ) , was studied . ^^^ The exposure of C 6 cells to N2ACM transiently stimulated the expression of both the MAG specific and the PLP specific messages up to eight and four fold over the control values , respectively . ^^^ The maximal upregulation of the PLP gene occurred two days after N2ACM administration and preceded that of the MAG gene by two days . ^^^ The optimal conditioning time for the release of the PLP gene stimulating activity was one day , while the maximal MAG gene stimulating activity was found in the medium conditioned for 3 days . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Although the total RNA content per cell was not affected by the AA treatment , AA upregulated the expression of myelin specific genes , i . e . proteolipid protein ( PLP ) and myelin associated glycoprotein ( MAG ) genes as assessed by northern blot analysis . ^^^ Three days after AA administration the message level reached a maximum of 10 and 2 fold over control for the PLP and MAG genes , respectively . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Analysis of B cell responses in blood showed low numbers of cells secreting IgG , IgA , or IgM antibodies against MBP , PLP , myelin associated glycoprotein , and myelin oligodendrocyte glycoprotein both in MS affected and unaffected family members . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| The expression of myelin specific protein genes , i . e . myelin proteolipid ( PLP ) , basic ( BP ) , and myelin associated glycoproteins ( MAG ) was studied in normal and severely undernourished 20 day old rats . ^^^ In contrast , the steady state levels of myelin specific mRNAs were significantly decreased by approximately 40 , 20 and 40 % for PLP , BP and MAG , respectively . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| The effect of retinoic acid ( RA ) on the expression of myelin specific genes , i . e . , proteolipid protein ( PLP ) and myelin associated glycoprotein ( MAG ) in rat glioma C 6 cells , was analyzed by Northern blot hybridization . ^^^ Furthermore , the reciprocal response of PLP versus MAG genes to RA demonstrates that these two genes utilize different regulatory mechanisms . . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| A model culture system of C 6 rat glioma cells was used to test the involvement of cAMP in the regulation of the myelin PLP and MAG genes . ^^^ The treatment of cells with isoproterenol ( 10 ( 5 ) to 10 ( 8 ) M ) upregulated the expression of the PLP and MAG genes in a concentration dependent manner . ^^^ However , the dissimilarity in the timing and the extent of upregulation of the PLP and MAG genes by cAMP stimulating agents indicate the existence of different intracellular mechanisms for the activation of these two genes . ^^^ Cycloheximide blocked the stimulatory effect of isoproterenol on both the PLP and MAG genes , indicating that the effect of cAMP on the myelin genes is mediated by protein product ( s ) of other cAMP response gene ( s ) . . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Early myelination in zitter rat was investigated by light and electron microscopic observations , by immunostaining for myelin basic protein ( MBP ) , proteolipid protein ( PLP ) and myelin associated glycoprotein ( MAG ) , and by morphometric analysis from the 1st to the 28th day of age . ^^^ However , the fundamental structure of the myelin lamellae appeared to be normal and the immunoreactivities for MBP , PLP and MAG were slightly delayed and weakend in comparison with age matched controls . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Relative to myelin purified from the same starting material , the 0 . 8M subfraction was enriched in MAG , CNPase , carbonic anhydrase and Na+ , K+ ATPase but was extremely low in PLP and MBP . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| T 3 increases the concentrations of mRNA transcribed from the following four myelin protein genes : myelin basic protein ( Mbp ) , myelin associated glycoprotein ( Mag ) , proteolipid protein ( Plp ) , and 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( Cnp ) . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| We have used this technique to detect and locate mRNA for myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNPase ) and myelin associated glycoprotein ( MAG ) in oligodendrocytes of 7 day old and adult rat brains . ^^^ PLP and MAG mRNA were restricted to the perinuclear cytoplasm , whereas MBP and CNPase mRNA was additionally present in peripheral oligodendrocyte processes . . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| We determined the temporal profile of expression of the genes encoding the P 0 glycoprotein , the myelin associated glycoprotein ( MAG ) , the myelin basic protein ( MBP ) , the proteolipid protein ( PLP ) , and 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNP ) in the sciatic nerve of rats . ^^^ P 0 and MBP RNA were present at very high levels , whereas the amount of MAG , PLP and CNP RNA were much less . . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Cellular RNA was extracted from transected and age matched control optic nerves 5 , 10 , 20 , and 40 days after surgery , and levels of the following mRNAs were determined by slot blot procedures : PLP , MBP , myelin associated glycoprotein ( MAG ) , and 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNP ) . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| A nonradioactive in situ hybridization procedure was employed to study the distribution of mRNAs for myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , myelin associated glycoprotein ( MAG ) and 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNP ) in oligodendrocytes in primary cultures of mouse brain . ^^^ The mRNAs for PLP and MAG remained confined to the oligodendrocyte somas at all times in culture at least up to 28 days . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| The level of PLP messages was reduced approximately 40 % in the quiescent Schwann cells in the distal segment of the sciatic nerve at 21 days after permanent transection , yet P 0 mRNA levels were very low , and MAG mRNAs were undetectable in this tissue . ^^^ These results indicate that myelin specific P 0 and MAG genes are tightly controlled at the level of transcription through Schwann cell axonal interactions , whereas PLP transcription in the peripheral nerve remains nearly dissociated from axonal influences . . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Umbilical cord blood of newborns and peripheral blood of healthy adults were investigated by an immunospot assay for cells secreting IgG , IgA and IgM antibodies against myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , myelin associated glycoprotein ( MAG ) and myelin oligodendrocyte glycoprotein ( MOG ) which represent putative antigens for an autoimmune attack in multiple sclerosis ( MS ) and against acetylcholine receptor ( AChR ) which is considered an important autoantigen in myasthenia gravis . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Oligodendrocytes , the myelin forming cells of the central nervous system , were cultured from newborn rat brain and optic nerve to allow us to analyze whether two transmembranous myelin proteins , myelin associated glycoprotein ( MAG ) and proteolipid protein ( PLP ) , were expressed together with myelin basic protein ( MBP ) in defined medium with low serum and in the absence of neurons . ^^^ We found that a proportion of oligodendrocytes derived from brain and optic nerve invariably express MBP , MAG , and PLP about a week after the emergence of GC , which occurs around birth . ^^^ In brain derived oligodendrocytes , MBP and MAG first emerge between the fifth and the seventh day after birth , followed by PLP 1 to 2 d later . ^^^ Each protein shows a specific cytoplasmic localization : diffuse for MBP , mostly perinuclear for MAG , and particulate for PLP . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| In a periplaque region and NAWM from the most rapidly progressing case of multiple sclerosis examined , the MAG content was between 30 and 35 % of the control level , whereas BP and PLP levels and CNP activity were between 50 and 85 % of control values . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Myelin associated glycoprotein ( MAG ) , 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNPase ) activity , myelin basic protein ( BP ) , and proteolipid protein ( PLP ) were quantitated in the brains of 20 day old Jimpy and control mice . ^^^ Myelin associated glycoprotein ( MAG ) , 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNPase ) activity , myelin basic protein ( BP ) , and proteolipid protein ( PLP ) were quantitated in the brains of 20 day old Jimpy and control mice . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| The antigens investigated were myelin associated glycoprotein ( MAG ) and proteolipid protein ( PLP ) . ^^^ Lymphocyte stimulation indices in active MS patients that were greater than 2 standard deviations above controls were as follows : 9 / 30 for MAG , 0 / 17 for PLP , and 8 / 81 for MBP . ^^^ No control subjects responded to MAG or PLP , and only 1 / 29 control subjects responded to MBP . ^^^ The only statistically significant differences among the groups for all myelin antigens tested were the proportion of individuals with active MS vs . controls that responded to MAG ( P less than 0 . 05 ) , and OND vs . controls and active MS that responded to PLP ( P less than 0 . 025 ) . ^^^ These studies have demonstrated reactivity to MAG but not PLP in some patients with active MS , and reactivity to PLP in some patients with other neurologic diseases . . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Myelin basic protein ( BP ) , proteolipid protein ( PLP ) , myelin associated glycoprotein ( MAG ) , and 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNPase ) activity were quantitated in the brains and spinal cords of normal and myelin deficient ( md ) rats at 8 , 12 , 18 , and 25 days of age . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| We examined developmental changes of myelin associated glycoprotein ( MAG ) , basic protein ( BP ) , and proteolipid protein ( PLP ) in central nervous system myelin isolated from experimental hyperphenylalaninemic rats ( PKU rats ) and controls . ^^^ MAG in developing myelin was at a maximum in 18 day old rats and began to decrease in 21 day old rats , while PLP and BP in developing myelin increased at these developmental stages . ^^^ In myelin from 12 to 18 day old PKU rats , the ratio of each protein such as MAG , PLP , or BP to that of control was about 0 . 5 at 12 days , and increased to almost 1 . 0 at 18 days . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Myelin associated glycoprotein ( MAG ) , myelin basic protein ( MBP ) , and proteolipid protein ( PLP ) were quantitated by immunoassays in nine plaque , inner periplaque , outer periplaque , and normal appearing white matter regions from brains of five multiple sclerosis patients and compared with the levels found in white matter samples of control subjects matched for age , postmortem time , and brain region . ^^^ In outer periplaque regions , MBP and PLP were close to control levels , but MAG was significantly reduced to a mean of 57 % of control . ^^^ Myelin associated glycoprotein ( MAG ) , myelin basic protein ( MBP ) , and proteolipid protein ( PLP ) were quantitated by immunoassays in nine plaque , inner periplaque , outer periplaque , and normal appearing white matter regions from brains of five multiple sclerosis patients and compared with the levels found in white matter samples of control subjects matched for age , postmortem time , and brain region . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Vibratome sections of brain were incubated with antisera to myelin basic protein ( MBP ) , myelin associated glycoprotein ( MAG ) , 2 ' : 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNP ) ( EC 3 . 1 . 4 . 37 ) , PLP , a synthetic PLP peptide , glial fibrillary acidic protein ( GFAP ) , and transferrin . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| We analyzed the location and abundance of transcripts for the 4 CNS myelin protein genes , myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , myelin associated glycoprotein ( MAG ) , and 2 ' , 3 ' cyclic nucleotide phosphohydrolase ( CNP ) , in the mouse cervical spinal cord from the time of rapid myelination until adulthood ( 8 45 d ) . ^^^ PLP , MAG , and CNP transcripts were clustered in the oligodendrocyte cell body , while MBP mRNAs were scattered throughout the cell body and processes . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Labeling the cells with various precursors followed by isolation of plasmalemma revealed that newly synthesized PLP , DM 20 , CNPase , and MAG were incorporated into the plasma membrane of `` floating ' ' oligodendrocytes ( i . e . , nonattached to substratum ) . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| The expression of myelin protein genes in Schwann cells has been studied in situ hybridization . 35S UTP labelled , antisense and sense RNA probes to the major protein Po , myelin basic protein ( MBP ) , myelin associated glycoprotein ( MAG ) and proteolipid protein ( PLP ) were employed with paraffin embedded sections , teased fibres and dissociated Schwann cells from sciatic nerves of rats . ^^^ The expression of MAG and PLP mRNA was markedly lower than Po and MBP . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| The proteolipid protein ( PLP ) , myelin associated glycoprotein ( MAG ) , myelin oligodendrocyte glycoprotein ( MOG ) and P 0 extracted from myelin were found exclusively in the detergent phase which is characteristic of the intrinsic membrane proteins . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| RNA was extracted from the brains of 18 and 27 day old mice and analyzed by northern blot using cDNA probes for proteolipid protein ( PLP ) , basic protein ( BP ) , and myelin associated glycoprotein ( MAG ) . ^^^ Two PLP transcripts of 3 . 2 and 2 . 4 kb ( kilobase ) were found , whereas PB and MAG probes hybridized to single regions of 2 . 2 and 2 . 5 kb , respectively . ^^^ Over this 9 day period the level of PLP and BP message in the control brain decreased by approximately 10 % , whereas the level of MAG message decreased by approximately 50 % . ^^^ The results indicate a reduced expression of the PLP and BP genes and a developmental delay in the mutant , whereas the genetic expression of MAG is enhanced and appears to be progressively dysregulated . . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Although T cell responses to the quantitatively major myelin proteins , myelin basic protein ( MBP ) and proteolipid protein ( PLP ) , are likely to be of importance in the course of multiple sclerosis ( MS ) , cell mediated autoimmune responses to other myelin antigens , in particular quantitatively minor myelin antigens , such as myelin associated glycoprotein ( MAG ) and the central nervous system specific myelin oligodendrocyte glycoprotein ( MOG ) , could also play a prevalent role in disease initiation or progression . ^^^ Highly purified myelin antigens were used in this study to assess cell mediated immune response to MOG in MS patients , in the context of the reactivity to other myelin antigens , MBP , PLP , and MAG . ^^^ The incidence of responses to MBP , PLP , and MAG did not differ greatly between MS patients and control individuals . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| The effect of dexamethasone on the expression of proteolipid protein ( PLP ) and myelin associated glycoprotein ( MAG ) genes was investigated in rat C 6 glioma cells . ^^^ The treatment of cells with dexamethasone transiently upregulated the expression of both genes with peak mRNA levels of approximately 10 fold over control levels occurring at day 3 for the PLP gene and at day 5 for the MAG gene . ^^^ Combined exposure of the cells to dexamethasone and retinoic acid featured an additive effect on PLP gene expression , whereas MAG gene expression was depressed below detectability level . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| The expression of the SCIP and myelin genes ( PLP , BP and MAG ) was quantitated by Northern blot analysis during early postnatal rat brain development . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Abnormal T cell responses to myelin components including myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , and myelin associated glycoprotein ( MAG ) have been implicated in the pathogenesis of multiple sclerosis . ^^^ Untreated patients with optic neuritis and multiple sclerosis had similarly raised levels of T cells recognising MBP , PLP , and MAG in peripheral blood . ^^^ The role of raised MBP , PLP , and MAG reactive Th 1 like cells found in optic neuritis and multiple sclerosis remains unexplained . . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| The elution was monitored of the two major protein components , i . e . myelin basic protein ( MBP ) and proteolipid protein ( PLP ) , as well as of minor components , viz . myelin associated glycoprotein ( MAG ) and myelin / oligodendrocyte glycoprotein ( MOG ) . ^^^ Whereas MBP and MOG elute as single sharp protein peaks upon chromatography , PLP and MAG are resolved into several different components . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Peptide sequences from human myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , myelin associated glycoprotein ( MAG ) , and myelin oligodendrocyte glycoprotein have been identified that bind to and form stable complexes with HLA A 2 . ^^^ MBP 110 118 , PLP 80 88 , MAG 287 295 , MAG 509 517 , and MAG 556 564 were all able to induce peptide specific HLA A 2 restricted CD8+ cytotoxic T lymphocyte ( CTL ) responses in vitro in HLA A2+ individuals . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Protein level immunofluorescence analysis by confocal microscopy reveals that in PLP minus mice there is a complete absence of PLP , a scattered appearance of MBP , and MAG expressed more widely in regions lacking MBP staining , which may be a compensatory mechanism . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| PKC inhibition resulting from long term exposure to 4 beta phorbol 12 , 13 dibutyrate ( 4 beta PDB ) reduced steady state levels of MBP , PLP , MAG , CNP , and PKC alpha mRNAs , as detected by slot blots or in situ hybridization , and downregulated the oligodendrocyte specific markers O 4 , galactosyl cerebroside , and the major constituent proteins MBP and PLP , as detected by immunocytochemistry . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| In well nourished forebrain , proteolipid protein ( PLP ) , myelin associated glycoprotein ( MAG ) , and basic protein ( BP ) messages began to increase polynomially after day 8 post partum , leading to a rapid accumulation of message during the following several days . ^^^ In undernourished forebrain , PLP , MAG , and BP messages did not show any increase until day 10 , and then increased at a diminished rate as compared to well nourished forebrain . ^^^ This study shows a pattern of temporal specificity when the myelin PLP , MAG , and BP genes are synchronously upregulated in the normal forebrain to a high rate of transcription between day 7 to 9 , which is several days before the onset of rapid myelination of the brain . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| MAG , myelin basic protein ( MBP ) , 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNP ) , proteolipid protein ( PLP ) and glial fibrillary acidic protein ( GFAP ) were quantified by western blot analysis in whole homogenates of spinal cords and hemispheres from affected goats at 1 , 3 and 6 days of age and from normal controls . ^^^ In mutant spinal cords , the deficits of MAG , CNP and PLP measured in whole homogenates corresponded reasonably well with the decreased myelin yields , but the levels of MBP were consistently much closer to control levels than those of the other myelin proteins . ^^^ In homogenates of mutant hemispheres , MAG , MBP , PLP and CNP were undetectable or at trace levels in comparison to controls . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , myelin associated glycoprotein ( MAG ) , and 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNP ) were measured by densitometric scanning of western blots . ^^^ A common finding in all regions examined was that MBP and PLP were reduced more than MAG and CNP . ^^^ This is probably explained by a greater deficit of compact myelin , in which MBP and PLP are localized , than of associated oligodendroglial membranes , in which MAG and CNP are concentrated . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| MS is characterized by elevated numbers of T cells recognizing MBP , PLP , MAG and MOG as well as the synthetic MBP amino acid sequences 1 20 , 63 88 , 89 101 , 96 118 , 110 128 and 148 165 , without immunodominance for any of these components . ^^^ PLP , MOG and MAG reactive T cells are strongly enriched in the patients ' CSF , as previously also shown for MBP reactive T cells . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Because proteins can serve as excellent markers for the myelin formation process , we examined the developmental expression of several important myelin proteins ( PLP / DM 20 , MBP , CNP , MAG , and MOG ) in both pt mutant and control rabbit brain homogenates . ^^^ Expression of the investigated proteins occurs in rabbits as follows : CNP and MAG are already present at the early postnatal stage ; PLP / DM 20 and MBP appear about the 10th postnatal day ; MOG , expressed last , has been detected on the 28th postnatal day . ^^^ Whereas the MBP , CNP , MAG , and MOG content is only slightly reduced in mature pt mutant brain homogenates ( 80 90 % of control values ) , the amount of PLP corresponds to approximately 30 40 % of that present in controls . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| In our previous work , we presented developmental studies on the expression of several myelin specific proteins ( PLP / DM 20 , MBP , CNP , MAG , and MOG ) in the brain homogeates of both pt mutant and age matched control Chinchilla rabbits aged 1 120 postnatal days . ^^^ Whereas the content of CNP , MBP , and MAG in isolated myelin membrane fractions seemed close to normal , a drastic PLP deficiency was observed . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Within 6 days in vitro they form extensive membranous sheets and acquire myelin associated glycoprotein ( MAG ) , proteolipid protein ( PLP ) , and myelin basic protein ( MBP ) . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| However , the temporospatial relationship of expression of two other major myelin proteins , proteolipid protein ( PLP ) and myelin associated glycoprotein ( MAG ) to that of MBP during fetal development has not been assessed in human tissues . ^^^ PLP and MAG were detected rostrally between 12 to 14 GW . ^^^ By the late second trimester , expression of MBP , PLP and MAG was noted in all locations in the spinal white matter except for the corticospinal tract . ^^^ The results suggest that PLP and MAG are expressed later than MBP but follow similar spatial gradients . . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Of the known Schwann cell proteins , MBP , P 2 , MAG , PLP , PO , PMP 22 and Connexin 32 , gene defects of the latter three have recently been shown to be responsible for some forms of hereditary sensory and motor neuropathies . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Our previous results demonstrated that low levels of myelin associated glycoprotein ( MAG ) , 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNP ) and proteolipid protein ( PLP ) found in CNS samples correlated with decreased yields of myelin . ^^^ The levels of mRNA for MAG , MBP and PLP in affected goat spinal cords compared with those of controls were equally decreased to approximately 50 % for the three myelin proteins . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Whole mounts of optic nerve , chiasm , and optic tract were sectioned horizontally and incubated with antibodies to myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , myelin associated glycoprotein ( MAG ) , `` Rip , ' ' and the neurite inhibitory protein ( IN 1 ) , followed by visualization with diaminobenzidine and a peroxidase conjugated secondary antibody . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| As analyzed by indirect immunofluorescence , the A2B5 surface marker is absent , they express galactocerebroside and myelin specific proteins , such as myelin basic protein ( MBP ) , myelin associated glycoprotein ( MAG ) , proteolipidprotein ( PLP ) , and Wolfgram protein ( WP ) , but do not exhibit astrocytic properties , such as the expression of vimentin or the glial fibrillary acidic protein ( GFAP ) . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Using in situ hybridization of cultured rat OLG we found that mRNAs are localized to at least three different locations : 1 ) to the perinuclear region [ myelin associated glycoprotein ( MAG ) mRNA ] ; 2 ) mainly to the processes ( the mRNA for the 14 kDa isoform of MBP ) ; and 3 ) to both the perinuclear region and the primary processes [ 2 ' , 3 ' cyclic nucleotide phosphodiesterase ( CNPase ) and proteolipid protein ( PLP ) mRNAs ] . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| To assess the role of thyroid hormone on myelin gene expression , we have studied the effect of hypothyroidism on the mRNA steady state levels for the major myelin protein genes : myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , myelin associated glycoprotein ( MAG ) and 2 ' : 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNP ) in different rat brain regions , during the first postnatal month . ^^^ |
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| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| The cellular / regional expression of myelin specific proteins : PLP , MBP , CNP ase , MAG and MOG was investigated in the brains of 14 and 42 days old control and pt mutant rabbits . ^^^ |
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| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Plasma membrane processes of oligodendrocytes deficient in PLP and MBP , but not in myelin associated glycoprotein ( MAG ) , spirally wrap large diameter axons , tightly adhering at their extracytosolic surfaces and forming a pseudo compacted myelin . ^^^ |
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| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| The perturbation in trafficking observed for altered Plp ( jp ) PLP and DM 20 in oligodendrocytes does not extend to other myelin membrane proteins , such as MAG and MOG , nor to wild type PLP co expressed in the same cell , all of which are correctly inserted into myelin . ^^^ |
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| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemical staining methods and antibodies against bovine proteolipid protein ( PLP ) , synthetic tridecapeptide of bovine PLP , human myelin basic protein ( MBP ) , human myelin associated glycoprotein ( MAG ) ; and 2 . ^^^ |
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| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Concomitantly , as investigated by RT PCR analysis , the transcriptional activity of c fos and c jun was stimulated , without altering mRNA expression of the myelin specific genes MBP , MAG , and PLP . ^^^ |
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| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| We performed linkage analysis of MS to markers in chromosomal regions that include the genes encoding myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , myelin associated glycoprotein ( MAG ) , oligodendrocyte myelin glycoprotein ( OMGP ) , and myelin oligodendrocyte glycoprotein ( MOG ) in a well characterized population of 65 multiplex MS families consisting of 399 total individuals , 169 affected with MS and 102 affected sibpairs . ^^^ Physical mapping data permitted placement of MAG and PLP genes on the Genethon genetic map ; all other genes were mapped on the Genethon genetic map by linkage analysis . ^^^ Results indicate that MAG , MBP , OMGP , and PLP genes do not have a significant genetic effect on susceptibility to MS in this population . ^^^ |
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| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Others have indicated that myelin composition may also vary with respect to myelin basic protein ( MBP ) and proteolipid protein ( PLP ) , and the small ( S ) and large ( L ) isoforms of myelin associated glycoprotein ( MAG ) . ^^^ In this study , we have determined the expression of myelin proteins in oligodendrocyte unit phenotypes 1 4 , which myelinate fibres ranging in diameter from 0 . 3 12 microns diameter in the AMV , by using double immunolabelling for Rip , which labels entire units , and MBP , PLP , myelin oligodendrocyte glycoprotein ( MOG ) , L MAG and S MAG . ^^^ |
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| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| The expression of major myelin protein genes , i . e . , proteolipid protein ( PLP ) , basic protein ( BP ) and myelin associated glycoprotein ( MAG ) were monitored by Northern blot analysis . ^^^ |
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| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Antisense oligodeoxynucleotides targeted to MAG mRNA profoundly alter BP and PLP mRNA expression in differentiating oligodendrocytes : a caution . ^^^ After four days , steady state levels of MAG , proteolipid protein ( PLP ) and basic protein ( BP ) mRNAs were determined by Northern blot analysis . ^^^ |
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| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Although all tested proteins could be floated to a low density fraction , proteolipid protein ( PLP ) , myelin basic protein ( MBP ) and myelin associated glycoprotein ( MAG ) were solubilized by the detergent extraction , and connexin 32 ( Cx 32 ) and oligodendrocyte specific protein ( OSP ) met only some of the criteria for DIGCEMs . ^^^ |
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| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Consistent with this hypothesis , Schwann cells in adult P 0 / mice display a novel molecular phenotype : PMP 22 expression is down regulated , MAG and PLP expression are up regulated , and MBP expression is unchanged . ^^^ In addition to these changes in gene expression in the P 0 knockout , PLP / DM 20 accumulates in the endoplasmic reticulum of P 0 / Schwann cells , whereas MAG accumulates in redundant loops of uncompacted myelin , not at nodes of Ranvier or Schmidt Lantermann incisures . ^^^ |
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| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Early onset of axonal degeneration in double ( plp / mag / ) and hypomyelinosis in triple ( plp / mbp / mag / ) mutant mice . ^^^ Double ( plp / mag / ) and triple ( plp / mbp / mag / ) null allelic mouse lines deficient in proteolipid protein ( PLP ) , myelin associated glycoprotein ( MAG ) , and myelin basic protein ( MBP ) were generated and characterized genetically , biochemically , and morphologically including their behavioral capacities . ^^^ The plp / mag / mutant develops a rapidly progressing axon degeneration in CNS with severe cognitive and motor coordinative deficits but has a normal longevity . ^^^ CNS axons of the plp / mbp / mag / mouse are hypomyelinated and ensheathed by `` pseudomyelin ' ' with disturbed protein and complex lipid composition . ^^^ The shiverer trait in the plp / mbp / mag / similar to the plp / mbp / mutant is significantly ameliorated , and its lifespan is considerably prolonged . ^^^ |
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| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemical analysis of taiep brains and spinal cords revealed a gradual decrease in levels of several myelin proteins including myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , myelin associated glycoprotein ( MAG ) , and 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase . ^^^ Accompanying early declines in MAG and PLP , accumulations of immunoreactive products were detected within oligodendrocytes , consistent with a defect in protein trafficking . ^^^ |
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| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| This was correlated with markedly reduced expression of the myelin proteins , proteolipid protein ( PLP ) , myelin basic protein ( MBP ) , 2 ' , 3 cyclic nucleotide 3 ' phosphodiesterase , and both large ( L ) and small ( S ) isoforms of myelin associated glycoprotein ( MAG ) . ^^^ Moreover , PLP and L MAG , which are dependent on the microtubule system for intracellular transport , accumulated in the perinuclear cytoplasm of the taiep oligodendrocyte . ^^^ |
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| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| MBP , MOG , PLP , MAG ) , the presence of reactive T cells in blood and , further enriched , in the cerebrospinal fluid ( CSF ) , large numbers of B cells in the CSF secreting antibodies of multiple but unknown specificities , an increase of mononuclear cells ( MNC ) expressing and secreting both pro and anti inflammatory cytokines , including Th 1 cytokines interferon gamma ( IFN gamma ) and interleukin ( IL ) 6 , the Th 2 related IL 4 and IL 10 , and the Th 3 driven TGF beta , elevated numbers of MNC in both blood and CSF expressing a spectrum of metalloproteinases and their inhibitors , as well as many other aberrations . ^^^ Furthermore , a complete epitope spread affecting MBP , MOG , PLP , MAG and other myelin components is already present in ON . ^^^ |
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| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Here we show that in rats that overexpress PLP , impairment of PLP transport from the cell body to the processes interferes with the translocation of other membrane proteins such as myelin associated glycoprotein ( MAG ) and myelin oligodendrocyte glycoprotein ( MOG ) , but not with peripherally translated MBP . ^^^ |
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| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| The lack of PLP is accompanied by deficits in the other myelin proteins of the central nervous system , including myelin basic protein , myelin associated glycoprotein , and cyclic nucleotide phosphodiesterase . ^^^ |
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| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| An immunohistochemical study , which can detect the specific expression of PLP , myelin basic protein , myelin associated glycoprotein , and platelet derived growth factor receptor alpha subunit in brain tissues , showed that the myelination was almost the same as that of age matched controls . ^^^ |
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| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Myelin oligodendrocyte glycoprotein ( MOG ) , proteolipid protein ( PLP ) , MAPK , and some phosphotyrosine containing proteins were found to co cluster with GalC and MBP , but myelin associated glycoprotein ( MAG ) and phosphatidylinositol 4 , 5 bisphosphate ( PIP ( 2 ) ) did not . ^^^ These results suggest that MOG and PLP , but probably not MAG , are possible candidates for transmembrane transmission of the signal received by GalC / CBS . ^^^ |
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| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| These antibodies , which recognize myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , myelin associated glycoprotein ( MAG ) , myelin oligodendrocyte glycoprotein ( MOG ) , oligodendrocyte specific protein ( OSP ) , 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNP ) , and transaldolase ( TAL ) , have been identified mostly in cerebrospinal fluid and in serum . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemical studies revealed significantly reduced levels of PLP / DM20 and other major myelin components such as myelin basic protein ( MBP ) , myelin associated glycoprotein ( MAG ) , and 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNP ) in shp myelin . ^^^ |
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| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| In the brain of 14 day old transgenic mice , the DM 20 mRNA level was decreased , whereas the PLP , MBP , CNP , and MAG mRNA levels were increased . ^^^ |
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| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| These results were confirmed by : ( 1 ) myelin basic protein ( MBP ) immunohistochemistry ; ( 2 ) western blotting of myelin associated glycoprotein ( MAG ) and MBP ; ( 3 ) qRT PCR analyses of myelin associated oligodendrocytic basic protein ( MOBP ) , MAG , MBP and proteolipid protein ( PLP ) in hippocampus , cortex and spinal cord ; ( 4 ) quantitation of ethanolamine and choline plasmalogens , all core myelin components ; ( 5 ) evaluation of myelin content in brain sections employing toluidine blue staining ; and ( 6 ) ultrastructural evaluation of myelin sheath thickness via electron microscopy . ^^^ |
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| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| In contrast , the levels of other myelin proteins , such as PLP and myelin associated glycoprotein ( MAG ) are decreased in the Id 4 null mice . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Among the most consistent changes in genes with robust expression were significant decreases in the expression of myelination related genes MAG , PLLP ( TM4SF11 ) , PLP 1 , ERBB 3 in subjects with schizophrenia . ^^^ |
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| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| At the molecular level , we find that Olig 1 regulates transcription of the major myelin specific genes , Mbp , Plp 1 , and Mag , and suppresses expression of a major astrocyte specific gene , Gfap . ^^^ |
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| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| Here we show that human QKI mRNA levels can account for a high proportion ( 47 % ) of normal interindividual mRNA expression variation ( and covariation ) of six oligodendrocyte related genes ( PLP 1 , MAG , MBP , TF , SOX 10 , and CDKN1B ) in 55 human brain autopsy samples from individuals without psychiatric diagnoses . ^^^ In addition , the tightly coexpressed myelin related genes ( PLP 1 , MAG , and TF ) have decreased mRNA levels in 55 schizophrenic patients , as compared with 55 control individuals , and most of this difference ( 68 96 % ) can be explained by variation in the relative mRNA levels of QKI 7kb , the same QKI splice variant previously shown to be down regulated in patients with schizophrenia . ^^^ |
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| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| One case of each of the following human leukodystrophies was examined immunocytochemically with antisera against myelin basic protein ( MBP ) , myelin associated glycoprotein ( MAG ) and gliofibrillary acidic protein ( GFA ) : Metachromatic leukodystrophy ( MLD ) , connatal adrenoleukodystrophy ( ALD ) , sudanophilic leukodystrophy of the adult ( SLD ) and connatal Pelizaeus Merzbacher disease ( PMD ) . ^^^ |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P60201 and P20916 |
Pubmed |
SVM Score :0.0 |
| NA |
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