| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :1.0977769 |
| A 73 residue C terminal region of Rabaptin 5 is necessary and sufficient both for the interaction with Rab 5 and for Rab 5 dependent recruitment of the protein on early endosomes . 1.0977769^^^ |
|
| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :0.64361037 |
| In contrast , rab 5 was as expected , shown to bind rabaptin 5 and rabex 5 as well as the endosomal effector protein EEA 1 but not GM 130 . 0.64361037^^^ |
|
| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :0.54351818 |
| Rabaptin 5 interacts with Rab 5 and is an essential component of the fusion machinery for targeting endocytic vesicles to early endosomes . 0.54351818^^^ |
|
| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :0.0 |
| Nucleotide triphosphate hydrolysis by Rab 5 is not conditional on membrane fusion and is reduced by its effector Rabaptin 5 . ^^^ |
|
| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :0.0 |
| Rabaptin 5 also binds the active form of GTPase Rab 5 . ^^^ |
|
| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :0.0 |
| Rab 15 does not interact directly with the Rab 5 effectors rabex 5 and rabaptin 5 in a yeast two hybrid binding assay . ^^^ |
|
| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :0.0 |
| Rabaptin 5 is , in turn , an effector of another small GTPase , Rab 5 , which regulates early endosome fusion . ^^^ |
|
| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :0.0 |
| We identified a rab4a binding domain in the N terminal region of rabaptin 4 , and two binding sites for rab 5 , including a novel N terminal rab5a binding site . ^^^ |
|
| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :0.0 |
| Rabaptin 5 is a direct effector of the small GTPase Rab 5 in endocytic membrane fusion . ^^^ We have identified a novel 100 kDa coiled coil protein , rabaptin 5 , that specifically interacts with the GTP form of the small GTPase Rab 5 , a potent regulator of endocytic transport . ^^^ Expression of a GTPase deficient Rab 5 mutant enhances the binding of rabaptin 5 to enlarged endosomes . ^^^ Rab 5 recruits rabaptin 5 to purified early endosomes in a GTP dependent manner , demonstrating functional similarities with other members of the Ras superfamily . ^^^ Immunodepletion of rabaptin 5 from cytosol strongly inhibits Rab 5 dependent early endosome fusion . ^^^ |
|
| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :0.0 |
| A novel Rab 5 GDP / GTP exchange factor complexed to Rabaptin 5 links nucleotide exchange to effector recruitment and function . ^^^ Rab GDP dissociation inhibitor delivers Rab 5 to the membrane , where a nucleotide exchange activity allows recruitment of an effector protein , Rabaptin 5 . ^^^ |
|
| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :0.0 |
| This protein shares 42 % sequence identity with Rabaptin 5 , a previously identified effector of Rab 5 , and we therefore named it Rabaptin 5beta . ^^^ Like Rabaptin 5 , Rabaptin 5beta displays heptad repeats characteristic of coiled coil proteins and is recruited on the endosomal membrane by Rab 5 in a GTP dependent manner . ^^^ Rabaptin 5beta does not heterodimerize with Rabaptin 5 , and forms a distinct complex with Rabex 5 , the GDP / GTP exchange factor for Rab 5 . ^^^ |
|
| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :0.0 |
| We show here that rabaptin 5 , an effector of the small GTPase Rab 5 that mediates membrane fusion in endocytosis , is one such protein . ^^^ |
|
| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :0.0 |
| Caspase dependent cleavage of rabaptin 5 generates two physically separated coiled coil forming domains , the C terminal of which retains the ability to bind the Rab 5 exchange factor rabex 5 . . ^^^ |
|
| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :0.0 |
| Several Rab 5 effectors are required in homotypic endosome fusion , including EEA 1 , which mediates endosome membrane docking , as well as Rabaptin 5 10 Rabex 5 complex and phosphatidylinositol 3 kinase hVPS 34 . ^^^ However , whereas Rab 5 and Rabaptin 5 are symmetrically distributed between CCVs and early endosomes , EEA 1 is recruited selectively onto the membrane of early endosomes . ^^^ |
|
| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :0.0 |
| Expression of the endocytosis regulatory proteins Rab 5 and Rabaptin 5 in glial cytoplasmic inclusions from brains with multiple system atrophy . ^^^ The present study examined whether or not aberrant expression of the endocytosis regulatory proteins Rab 5 and Rabaptin 5 occcur in oligodendrocytes of brains with MSA . ^^^ Immunohistochemistry was done using monoclonal anti Rab 5 and anti Rabaptin 5 antibodies based on ABC method . ^^^ RESULTS : We have observed Rab 5 and Rabaptin 5 immunoreactivity in the neuronal somata and axons of the controls , suggesting that Rab 5 and Rabaptin 5 are involved in the regulation of the endocytosis in neurons of the human central nervous system . ^^^ In the brain tissues from patients with MSA , we have found Rab 5 and Rabaptin 5 immunoreactivity in GCIs . ^^^ |
|
| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :0.0 |
| To evaluate endocytic activation , we used markers of internalization ( rab 5 , rabaptin 5 ) and recycling ( rab 4 ) , and found that enlargement of rab 5 positive early endosomes in the AD brain was associated with elevated levels of rab 4 immunoreactive protein and translocation of rabaptin 5 to endosomes , implying that both endocytic uptake and recycling are activated . ^^^ |
|
| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :0.0 |
| Functional synergy between Rab 5 effector Rabaptin 5 and exchange factor Rabex 5 when physically associated in a complex . ^^^ We have previously shown that Rab 5 , which regulates various steps of transport along the early endocytic pathway , is activated by a complex consisting of Rabex 5 , a Rab 5 nucleotide exchange factor , and the effector Rabaptin 5 . ^^^ First , we show that Rabaptin 5 increases the exchange activity of Rabex 5 on Rab 5 . ^^^ Second , Rab 5 dependent recruitment of Rabaptin 5 to early endosomes is completely dependent on its physical association with Rabex 5 . ^^^ |
|
| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :0.0 |
| Rabaptin 5 is a well studied protein shown to be an essential and rate limiting component of early endosomal fusion through interaction with the Ras family GTPases Rab 5 and Rab 4 . ^^^ |
|
| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :0.0 |
| In yeast two hybrid screening using gamma 1 adaptin , a subunit of the AP 1 adaptor complex of clathrin coated vesicles derived from the trans Golgi network ( TGN ) , as bait , we found that it could interact with Rabaptin 5 , an effector of Rab 5 and Rab 4 that regulates membrane docking with endosomes . ^^^ |
|
| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :0.0 |
| Here , we show that a genetic model of DS ( trisomy 21 ) , the segmental trisomy 16 mouse Ts65Dn , develops enlarged neuronal early endosomes , increased immunoreactivity for markers of endosome fusion ( rab 5 , early endosomal antigen 1 , and rabaptin 5 ) , and endosome recycling ( rab 4 ) similar to those in AD and DS individuals . ^^^ |
|
| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :0.0 |
| To visualize active , GTP loaded Rab 5 in living cells , we developed molecular sensors consisting of the Rab 5 binding fragments of Rabaptin 5 or EEA . 1 fused to yellow fluorescent protein ( YFP ) . ^^^ The resulting chimeric CFP Rab 5 CAAX was located mainly in the plasma membrane and was capable of binding GTP as judged by FRET microscopy with the Rabaptin 5 based sensor . ^^^ |
|
| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :0.0 |
| The Dot / Icm mediated activation of caspase 3 by L . pneumophila specifically cleaves , in a dose and time dependent manner , the Rab 5 effector Rabaptin 5 , which maintains Rab 5 GTP on the endosomal membrane . ^^^ |
|
| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :0.0 |
| We present here the crystal structure of the Rab 5 GTPase domain in complex with a GTP analog and the C terminal domain of effector Rabaptin 5 . ^^^ The proteins form a dyad symmetric Rab 5 Rabaptin5 ( 2 ) Rab 5 ternary complex with a parallel coiled coil Rabaptin 5 homodimer in the middle . ^^^ Two Rab 5 molecules bind independently to the Rabaptin 5 dimer using their switch and interswitch regions . ^^^ We also present the crystal structures of two distinct forms of GDP Rab 5 complexes , both of which are incompatible with Rabaptin 5 binding . ^^^ |
|
| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :0.0 |
| The Rab 5 effector Rabaptin 5 and its isoform Rabaptin 5delta differ in their ability to interact with the small GTPase Rab 4 . ^^^ Rabaptin 5 is an effector for the small GTPase Rab 5 , a regulator of the early steps in endocytosis . ^^^ We found that unlike Rabaptin 5 , that interacts with both GTPases in GTP bound conformations , Rabaptin 5delta interacts only with GTP bound Rab 5 , and does not interact with Rab 4 , presumably due to a disrupted Rab 4 binding site . ^^^ Our data suggests that while Rabaptin 5 was proposed to act as a molecular linker between Rab 5 and Rab 4 , to coordinate endocytic and recycling traffic , Rabaptin 5delta is involved only in the Rab 5 driven events . . ^^^ |
|
| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :0.0 |
| The structures are discussed with respect to the recently determined structure of human Rab5a and its complex with the Rab 5 binding domain of the bivalent effector Rabaptin 5 . ^^^ |
|
| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :0.0 |
| Rabex 5 forms a tight complex with Rabaptin 5 , a multivalent adaptor protein that also binds to Rab 4 , Rab 5 , and to domains present in gamma adaptins and the Golgi localized , gamma ear containing , ARF binding proteins ( GGAs ) . ^^^ Rabaptin 5 augments the Rabex 5 exchange activity , thus generating GTP bound , membrane associated Rab 5 that , in turn , binds Rabaptin 5 and stabilizes the Rabex 5 . ^^^ |
|
| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :0.0 |
| Rab 5 binding fragments of Rabaptin 5 or early endosomal antigen 1 ( EEA . 1 ) were fused to yellow fluorescent protein ( YFP ) and used in the fluorescent resonance energy transfer ( FRET ) assay together with Rab 5 tagged cyan fluorescent protein ( CFP ) . ^^^ |
|
| Interacting proteins: Q15276 and P20339 |
Pubmed |
SVM Score :0.0 |
| Apoptotic inhibition of endosomal transport occurs due to cleavage of Rabaptin 5 , an effector of small GTPase Rab 5 , which results in inhibition of early endosome fusion . ^^^ We also mapped a novel , N terminal , putative Rab 5 binding site in Rabaptin 5 like proteins , which becomes physically separated from the previously known C terminal Rab 5 binding site after apoptotic cleavage of these proteins . ^^^ Presence of the second Rab 5 binding site provides a new insight into Rabaptin 5 function in early endosome fusion and a mechanistic model for functional inactivation of Rabaptin 5 in apoptosis . . ^^^ |
|