| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :1.0091086 |
| Rabphilin 3A is known to bind with Rab3A , Rab3B , and Rab3C . 1.0091086^^^ |
|
| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.54164308 |
| Lipid modified GTP Rab3A ( Mr approximately 50 , 000 ) formed a heterotetramer ( Mr approximately 210 , 000 ) with two Rabphilin 3A molecules , whereas lipid unmodified GTP Rab3A formed a heterodimer ( Mr approximately 105 , 000 ) with one Rabphilin 3A molecule . 0.54164308^^^ |
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| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.57820689 |
| Finally , we studied the expression in insulin secreting cells of rabphilin 3A , a putative effector protein that associates with the GTP bound form of Rab3A . 0.57820689^^^ |
|
| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| Rabphilin 3A is a putative target protein for Rab3A small GTP binding protein implicated in neurotransmitter release . ^^^ |
|
| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| The binding of G proteins to Rab / GDP dissociation inhibitor , Rab3A / GTPase activating protein or rabphilin 3A was not affected by the prenylcysteine analogs . ^^^ |
|
| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| Rabphilin 3A is a putative target protein for Rab3A small GTP binding protein which is implicated in neurotransmitter release . ^^^ |
|
| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| Rabphilin 3A is associated with synaptic vesicles through a vesicle protein in a manner independent of Rab3A . ^^^ Rabphilin 3A is a putative target protein for Rab3A small GTP binding protein , which is implicated in regulated secretion , particularly in neurotransmitter release . ^^^ These vesicles deprived of rabphilin 3A still contained Rab3A and synaptophysin . ^^^ Addition of exogenous Rab3A or removal of endogenous Rab3A by the action of Rab GDI did not affect the binding of exogenous rabphilin 3A to the vesicles . ^^^ These results suggest that rabphilin 3A is associated with synaptic vesicles at least through a vesicle protein in a manner independent of Rab3A . . ^^^ |
|
| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| Rabphilin 3A is a putative target protein for Rab3A small GTP binding protein which is implicated in neurotransmitter release . ^^^ |
|
| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| Recently , Rabphilin 3A , a putative target protein for Rab3A , was identified and its cDNA has been cloned from bovine brain . ^^^ The amino acid identity between mouse and bovine Rabphilin 3A is highest in their carboxyl terminal halves ( 97 . 8 % identity ) and amino termini ( 93 . 0 % identity ) , which contain the region of the two internal repeats homologous to the regulatory domain ( C 2 domain ) of protein kinase C and the putative Rab3A binding region , respectively . ^^^ |
|
| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| Rabphilin 3A is a synaptic vesicle protein that interacts with rab3A in a GTP dependent manner and binds Ca2+ in a phospholipid dependent manner . ^^^ This identifies the middle region of rabphilin 3A situated between the N terminal rab3A binding sequences and the C terminal C 2 domains involved in Ca2+ / phospholipid binding as a regulatory domain . ^^^ |
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| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| GDP / GTP exchange reaction stimulating activity of Rabphilin 3A for Rab3A small GTP binding protein . ^^^ Rabphilin 3A is a putative target protein for Rab3A , a small GTP binding protein particularly implicated in neurotransmitter release . ^^^ Rabphilin 3A interacts more preferentially with GTP Rab3A than with GDP Rab3A . ^^^ Moreover , Rabphilin 3A shows a weak activity to stimulate the GTPase activity of Rab3A and a strong activity to inhibit the Rab3A GTPase activating protein ( GAP ) stimulated GTPase activity of Rab3A . ^^^ Here , we show that Rabphilin 3A has another activity to stimulate the GDP / GTP exchange reaction of Rab3A . ^^^ |
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| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of Rabphilin 3A , a putative target protein for Rab3A , by cyclic AMP dependent protein kinase . ^^^ Rab3A small GTP binding protein and its associated proteins , such as Rabphilin 3A , a putative target protein for Rab3A , MSS 4 , a stimulatory GDP / GTP exchange protein for Rab3A , and Rab GDI , a translocator for the Rab family members including Rab3A , are implicated in neurotransmitter release . ^^^ Neither Rab3A , MSS 4 , nor Rab GDI was phosphorylated , but Rabphilin 3A was phosphorylated Rab GDI was phosphorylated , but Rabphilin 3A was phosphorylated at its N terminal region . ^^^ |
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| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| Synaptic targeting of rabphilin 3A , a synaptic vesicle Ca2+ / phospholipid binding protein , depends on rab3A / 3C . rab3A , a low molecular weight GTP binding protein of synaptic vesicles with a putative function in synaptic vesicle docking , interacts in a GTP dependent manner with rabphilin 3A , a peripheral membrane protein that binds Ca2+ and phospholipids . ^^^ In rab3A deficient mice , rabphilin 3A is decreased in synapses belonging to neurons that primarily express rab3A and accumulates in the perikarya of these neurons . ^^^ These results suggest that analogous to the membrane recruitment of raf by ras , rab3A and rab3C may function in recruiting rabphilin 3A to the synaptic vesicle membrane in a GTP dependent manner . . ^^^ |
|
| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| Rabphilin 3A is a putative target protein for Rab3A small GTP binding protein which is implicated in neurotransmitter release . ^^^ |
|
| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| Rab3A GTPase activating protein inhibiting activity of Rabphilin 3A , a putative Rab3A target protein . ^^^ Rabphilin 3A is a putative target protein for Rab3A , a member of the small G protein superfamily that is implicated in regulated secretion , particularly in neurotransmitter release . ^^^ Rabphilin 3A contains at least two functionally different domains : the N terminal Rab3A binding domain and the C terminal C 2 domain , which interacts with both Ca2+ and phospholipid . ^^^ Because Rabphilin 3A interacts preferentially with GTP Rab3A rather than with GDP Rab3A , we have examined here whether Rabphilin 3A affects the GTPase activity of Rab3A . ^^^ Rabphilin 3A and its N terminal fragment , but not its C terminal fragment , very weakly stimulated the basal GTPase activity of Rab3A . ^^^ |
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| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| Two functionally different domains of rabphilin 3A , Rab3A p25 / smg p25A binding and phospholipid and Ca ( 2+ ) binding domains . ^^^ Rabphilin 3A is a putative target molecule for rab3A p25 / smg p25A , which is a member of a ras p 21 related small GTP binding protein and implicated in neurotransmitter release from the synapse . ^^^ The GTP gamma S bound form of rab3A p 25 bound to both rabphilin 3A and the N terminal fragment but did not bind to the C terminal fragment . ^^^ These results indicate that rabphilin 3A has at least two functionally different domains , the N terminal rab3A p 25 binding and C terminal phospholipid and Ca ( 2+ ) binding domains . . ^^^ |
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| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| Localization of rabphilin 3A , a putative target protein for Rab3A , at the sites of Ca ( 2+ ) dependent exocytosis in PC 12 cells . ^^^ We have recently identified a putative target protein for Rab3A , isolated its cDNA , and designated it as Rabphilin 3A . ^^^ To examine whether Rabphilin 3A as well as Rab3A is localized at the sites of Ca ( 2+ ) dependent exocytosis , we investigated here localization of Rabphilin 3A and Rab3A in comparison with the sites of exocytosis in the differentiated PC 12 cells . ^^^ Rabphilin 3A as well as Rab3A was highly concentrated at the tips of the neurites where Ca ( 2+ ) dependent exocytosis took place . ^^^ Inversely , neither Rabphilin 3A nor Rab3A was concentrated at the tips of the neurites where Ca ( 2+ ) dependent exocytosis did not take place . ^^^ |
|
| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| Rabphilin 3A , a putative target protein for smg p25A / rab3A p 25 small GTP binding protein related to synaptotagmin . ^^^ Moreover , both rabphilin 3A purified from bovine brain and recombinant rabphilin 3A made a complex with the GTP gamma S bound form of rab3A p 25 but not with the GDP bound form of rab3A p 25 . ^^^ These results indicate that rabphilin 3A is a novel protein that has C 2 domains and selectively interacts with the GTP bound form of rab3A p25 . . ^^^ |
|
| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| Rabphilin 3A , a putative target molecule of Rab3A small GTP binding protein implicated in Ca2+ dependent exocytosis , consists of two functionally different domains : the N terminal Rab3A binding domain and the C terminal two C 2 like domains ( C2A and C2B domains ) interacting with Ca2+ and phospholipid . ^^^ |
|
| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| The membrane association of rabphilin 3A was modestly enhanced in Rab3B expressing PC 12 cells relative to Rab3A overexpressing cells . ^^^ Our results indicate that Rab3A and Rab3B have similar membrane targeting properties and are capable of interacting with the same putative downstream effector ; i . e . rabphilin 3A . ^^^ |
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| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| Rabphilin 3A is one of the most attractive candidates for a Rab3a effector molecule in synaptic vesicle exocytosis . ^^^ The pattern for accumulation of rabphilin 3A was similar to that of Rab3a accumulation , including the poor recycling . ^^^ Double labelling showed a high degree of colocalization between Rab3a and rabphilin 3A in spinal roots and sciatic nerves . ^^^ Furthermore , as it colocalized in most , but not all , structures with Rab3a , results support the hypothesis that rabphilin 3A is interacting with Rab3a . . ^^^ |
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| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| Role of the Rab3A binding domain in targeting of rabphilin 3A to vesicle membranes of PC 12 cells . ^^^ A putative downstream target for Rab3A , rabphilin 3A , is located exclusively on secretory vesicle membranes . ^^^ |
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| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| Rabphilin 3A is a downstream target molecule of Rab3A small GTP binding protein and implicated in Ca2+ dependent neurotransmitter release . ^^^ Here we have isolated a rabphilin 3A interacting molecule from a human brain cDNA library by the yeast two hybrid method and identified it to be alpha actinin , known to cross link actin filaments into a bundle . alpha Actinin interacts with the N terminal region of rabphilin 3A , with which GTP Rab3A interacts , and this interaction stimulates the activity of alpha actinin to cross link actin filaments into a bundle . ^^^ The interaction of rabphilin 3A with alpha actinin is inhibited by guanosine 5 ' ( 3 O thio ) triphosphate Rab3A . ^^^ These results suggest that the Rab3A rabphilin 3A system regulates the alpha actinin regulated reorganization of actin filaments . ^^^ Therefore , rabphilin 3A may serve as a linker for Rab3A and cytoskeleton . . ^^^ |
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| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| Rabphilin 3A is a putative target protein for Rab3A , a member of the small GTP binding protein superfamily that has been suggested to play a role in regulated exocytosis in presynapses . ^^^ |
|
| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| Moreover , these results together with the fact that Rabphilin 3A associated with synaptic vesicles inhibits the activity of Rab 3 GTPase activating protein suggest that the GTP bound form of modified Rab3A is associated with synaptic vesicles through both Rabphilin 3A and the vesicle phospholipid . . ^^^ |
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| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| We have cloned a cDNA encoding a novel protein of 302 amino acids ( designated Noc 2 , no C 2 domain ) that has 40 . 7 % amino acid identity with and 77 . 9 % similarity to the N terminal region of rabphilin 3A , a target molecule of Rab3A . ^^^ |
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| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| Injection of fragments of Rabphilin 3A indicate that at least two distinct regions of the protein inhibit neurotransmitter release : the NH 2 terminal region that binds Rab3A and is phosphorylated by protein kinases and the two C 2 domains that interact with calcium , phospholipid , and beta adducin . ^^^ |
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| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| Structural basis of Rab effector specificity : crystal structure of the small G protein Rab3A complexed with the effector domain of rabphilin 3A . ^^^ The crystal structure of activated Rab3A / GTP / Mg2+ bound to the effector domain of rabphilin 3A was solved to 2 . 6 A resolution . ^^^ Rabphilin 3A contacts Rab3A in two distinct areas . ^^^ The second interface consists of a deep pocket in Rab3A that interacts with a SGAWFF structural element of rabphilin 3A . ^^^ |
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| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| A region with high homology between the Spir family proteins is located adjacent ( N terminal ) to the modified FYVE domain and is designated as `` Spir box . ' ' The Spir box has sequence similarity to a region of rabphilin 3A , which mediates interaction with the small GTPase Rab3A . ^^^ |
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| Interacting proteins: Q9Y2J0 and P20336 |
Pubmed |
SVM Score :0.0 |
| Glomerular podocytes possess the synaptic vesicle molecule Rab3A and its specific effector rabphilin 3a . ^^^ In neurons , the small guanosine triphosphatase Rab3A and its effector rabphilin 3A form a complex required for the correct docking of synaptic vesicles to their target membrane . ^^^ Because rabphilin 3A binds in neurons to cytoskeletal proteins also important for podocyte homeostasis , and the complex rabphilin 3A Rab3A has been demonstrated in neurons and neuroendocrine cells , the aim of our work was to investigate their possible expression and regulation in podocytes . ^^^ Normal kidneys from mouse , rat , and human were studied by immunohistochemistry , Western blotting , and reverse transcriptase polymerase chain reaction to evaluate the expression of Rab3A and rabphilin 3A . ^^^ Our results demonstrated that rabphilin 3A and Rab3A are present in normal mouse , rat , and human kidneys , with an exclusively glomerular expression and a comma like pattern of positivity along the glomerular capillary wall , suggestive for podocyte staining . ^^^ |
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