| Interacting proteins: Q86UR5 and P20336 |
Pubmed |
SVM Score :0.50074248 |
| In this Chapter , 1 describe several assay methods that have been used to determine the physiological significance of the alternative splicing event in the RBD 1 of Rim 2 , including assays for the in vitro interaction between Rim 2 RBD and Rab3A and for the localization of Rim 2 RBD on DCVs in PC 12 cells . . 0.50074248^^^ |
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| Interacting proteins: Q86UR5 and P20336 |
Pubmed |
SVM Score :0.0 |
| Rab3a binding and secretion enhancing domains in Rim 1 are separate and unique . ^^^ Rim 1 was identified in brain by its ability to bind Rab3a GTP and has been postulated to be a Rab3a effector protein . ^^^ We have investigated the structural basis for the ability of Rim 1 to bind Rab3a GTP and to stimulate exocytosis in chromaffin cells . ^^^ The abilities of Rim 1 to enhance secretion and to bind Rab3a GTP reside on distinct and relatively small domains that act independently . ^^^ The zinc finger domain in Rim 1 is unnecessary for Rab3a GTP binding but , alone , enhances secretion . ^^^ |
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| Interacting proteins: Q86UR5 and P20336 |
Pubmed |
SVM Score :0.0 |
| This Rab 3 binding site of Rim 1 is reminiscent of the N terminal alpha helix that is part of the Rab 3 binding region of rabphilin 3 , and indeed we observed low affinity , specific binding of Rab3A ( K ( d ) on the order of magnitude of 10 100 microm ) to this region of rabphilin 3 alone ( amino acids 40 88 ) , whereas additional sequences up to amino acid 178 are needed for high affinity Rab3A binding to rabphilin 3 ( K ( d ) = 10 20 nm ) . ^^^ In contrast , an N terminal alpha helix motif in aczonin , with sequence similarity to the Rab 3 binding site of Rim 1 , did not bind Rab3A , C , or D or several other Rab proteins . ^^^ |
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| Interacting proteins: Q86UR5 and P20336 |
Pubmed |
SVM Score :0.0 |
| Rim 1 has been identified in brain by its ability to bind Rab3a GTP and has been postulated to be a Rab3a effector protein . ^^^ Like Rabphilin 3 , another putative Rab3a effector protein , Rim 1 modulates secretion and contains a zinc finger and two C 2 domains . ^^^ We have investigated the structural basis for the ability of Rim 1 to bind Rab3a GTP and to stimulate exocytosis in chromaffin cells . . ^^^ |
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| Interacting proteins: Q86UR5 and P20336 |
Pubmed |
SVM Score :0.0 |
| In this study , 1 tested the interaction of rabphilin , Rim 1 , Rim 2 , and Noc 2 with 42 different Rab proteins by cotransfection assay and found differences in rabphilin , Rim 1 , Rim 2 , and Noc 2 binding to several Rab proteins that belong to the Rab functional group 3 ( Rab3A / B / C / D , Rab 26 , Rab27A / B , and Rab 37 ) and / or 8 ( Rab8A and Rab 10 ) . ^^^ Rim 1 interacts with Rab3A / B / C / D , Rab 10 , Rab 26 , and Rab 37 ; Rim 2 interacts with Rab3A / B / C / D and Rab8A ; and rabphilin and Noc 2 interact with Rab3A / B / C / D , Rab8A , and Rab27A / B . ^^^ |
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| Interacting proteins: Q86UR5 and P20336 |
Pubmed |
SVM Score :0.0 |
| Rim 1 , a brain specific Rab3a binding protein , localizes to the presynaptic cytomatrix and plays an important role in synaptic transmission and synaptic plasticity . ^^^ |
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| Interacting proteins: Q86UR5 and P20336 |
Pubmed |
SVM Score :0.0 |
| Rim 1 and Rim 2 were originally described as specific Rab3A effector proteins involved in the regulation of secretory vesicle exocytosis . ^^^ Similarly , Rim1Delta56 105 exhibited higher affinity binding to Rab3A than the long form of Rim 1 . ^^^ |
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| Interacting proteins: Q86UR5 and P20336 |
Pubmed |
SVM Score :0.0 |
| Rab3A probably acts via two effector proteins , rabphilin and RIM , of which rabphilin is an in vitro substrate for PKA . ^^^ |
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| Interacting proteins: Q86UR5 and P20336 |
Pubmed |
SVM Score :0.0 |
| Co expression of Rab3A reversed the effect of RIM on exocytosis . ^^^ |
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| Interacting proteins: Q86UR5 and P20336 |
Pubmed |
SVM Score :0.0 |
| The effector by which Rab3A exerts its inhibitory effect is unclear as the Rab3A effectors Rabphilin and RIM have been excluded from for this role . ^^^ |
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| Interacting proteins: Q86UR5 and P20336 |
Pubmed |
SVM Score :0.0 |
| Two putative rab3A effectors have been described in neurons , rabphilin which is a soluble protein that moves onto and off synaptic vesicles in concert with rab3A , and RIM which is an active zone protein that only binds to rab3A on docked vesicles . ^^^ |
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| Interacting proteins: Q86UR5 and P20336 |
Pubmed |
SVM Score :0.0 |
| Thus , neurosecretion involves two key processes : the docking of vesicles at the transmitter release site , a process that involves the scaffold protein RIM ( Rab3A interacting molecule ) and its binding partner Munc 13 , and the subsequent gating of vesicle fusion by activation of the Ca2+ channels . ^^^ |
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| Interacting proteins: Q86UR5 and P20336 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q86UR5 and P20336 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q86UR5 and P20336 |
Pubmed |
SVM Score :0.0 |
| NA |
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