| Interacting proteins: P52434 and P19388 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52434 and P19388 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52434 and P19388 |
Pubmed |
SVM Score :0.0 |
| The Saccharomyces cerevisiae genes that encode these subunits ( RPB 5 , RPB 6 , and RPB 8 ) were isolated and sequenced , and their transcriptional start sites were deduced . ^^^ RPB 5 encodes a 25 kD protein , RPB 6 , an 18 kD protein , and RPB 8 , a 16 kD protein . ^^^ |
|
| Interacting proteins: P52434 and P19388 |
Pubmed |
SVM Score :0.0 |
| We have found that in murine fibroblasts exposure to alpha amanitin triggered degradation of the RPB 1 subunit , while other RNAPII subunits , RPB 5 and RPB 8 , remained almost unaffected . ^^^ |
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| Interacting proteins: P52434 and P19388 |
Pubmed |
SVM Score :0.0 |
| Far Western blot analysis of S . pombe RNA polymerase 2 using 32P labelled recombinant Rpb 5 fused to glutathione S transferase ( GST ) as a probe , indicated that Rpb 5 binds strongly to membrane immobilized Rpb 1 , Rpb 2 and Rpb 3 and weakly to Rpb 5 and a 15 kDa subunit ( Rpb 8 or Rpb 11 ) . ^^^ |
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| Interacting proteins: P52434 and P19388 |
Pubmed |
SVM Score :0.0 |
| After successive affinity chromatography on glutathione and Ni ( 2+ ) columns , complexes consisting of the seven subunits , Rpb1H , Rpb 2 , GST Rpb 3 , Rpb 5 , Rpb 7 , Rpb 8 and Rpb 11 , were identified . ^^^ |
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| Interacting proteins: P52434 and P19388 |
Pubmed |
SVM Score :0.0 |
| The RNA polymerase 2 ( Pol 2 ) of the fission yeast Schizosaccharomyces pombe is composed of 12 different polypeptides , Rpb 1 to Rpb 12 , of which five , Rpb 5 , Rpb 6 , Rpb 8 , Rpb 10 and Rpb 12 , are shared among three forms of the RNA polymerase . ^^^ |
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| Interacting proteins: P52434 and P19388 |
Pubmed |
SVM Score :0.0 |
| The transcription of one group of genes including rpb 3 , rpb 4 , rpb 5 , rpb 6 , rpb 7 and rpb 10 is mainly initiated at a single site , while that of the other group of genes for rpb 1 , rpb 2 , rpb 8 , rpb 9 , rpb 11 and rpb 12 is initiated at multiple sites . ^^^ |
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| Interacting proteins: P52434 and P19388 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : The RNA polymerase 2 of the fission yeast Schizosaccharomyces pombe consists of 12 Rpb subunits , of which four ( Rpb 1 , Rpb 2 , Rpb 3 and Rpb 11 ) form the assembly and catalytic core and five ( Rpb 5 , Rpb 6 , Rpb 8 , Rpb 10 and Rpb 12 ) are shared among RNA polymerases 1 , 2 and 3 . ^^^ |
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| Interacting proteins: P52434 and P19388 |
Pubmed |
SVM Score :0.0 |
| Four subunits of Schizosaccharomyces pombe RNA polymerases 1 3 shared by all three enzymes ( Rpb 5 , Rpb 8 , Rpb 10 and Rpc 10 [ Rpb 12 ] ) have been overexpressed in Escherichia coli expression vectors pQE or pET as hexahistidine fusions . ^^^ |
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| Interacting proteins: P52434 and P19388 |
Pubmed |
SVM Score :0.0 |
| Nine subunits homologous to the other eukaryotic RNA Pol 2 , namely RPB 1 , RPB 2 , RPB 3 , RPB 4 , RPB 5 , RPB 6 , RPB 7 , RPB 8 and RPB 11 , were identified in the purified complex . ^^^ |
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| Interacting proteins: P52434 and P19388 |
Pubmed |
SVM Score :0.0 |
| To assess functional relatedness of individual components of the eukaryotic transcription apparatus , three human subunits ( hsRPB 5 , hsRPB 8 , and hsRPB 10 ) were tested for their ability to support yeast cell growth in the absence of their essential yeast homologs . ^^^ |
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