| Interacting proteins: P19388 and P24928 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P19388 and P24928 |
Pubmed |
SVM Score :0.0 |
| In cross linking reactions dependent on transcription , RPB 1 , RPB 2 , and RPB 5 were cross linked with N ( 3 ) RdUTP . ^^^ |
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| Interacting proteins: P19388 and P24928 |
Pubmed |
SVM Score :0.0 |
| The cDNAs of five subunits ( hRPB 220 , hRPB 140 , hRPB 33 , hRPB 25 , and hRPB14 . 5 ) have been isolated . ^^^ |
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| Interacting proteins: P19388 and P24928 |
Pubmed |
SVM Score :0.0 |
| We have found that in murine fibroblasts exposure to alpha amanitin triggered degradation of the RPB 1 subunit , while other RNAPII subunits , RPB 5 and RPB 8 , remained almost unaffected . ^^^ |
|
| Interacting proteins: P19388 and P24928 |
Pubmed |
SVM Score :0.0 |
| Far Western blot analysis of S . pombe RNA polymerase 2 using 32P labelled recombinant Rpb 5 fused to glutathione S transferase ( GST ) as a probe , indicated that Rpb 5 binds strongly to membrane immobilized Rpb 1 , Rpb 2 and Rpb 3 and weakly to Rpb 5 and a 15 kDa subunit ( Rpb 8 or Rpb 11 ) . ^^^ In agreement with this result , the 32P labelled Rpb 3 probe showed a strong binding signal against Rpb 5 in addition to Rpb 1 and Rpb 2 . ^^^ |
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| Interacting proteins: P19388 and P24928 |
Pubmed |
SVM Score :0.0 |
| Previously , we cloned and sequenced both cDNA and the genes encoding the four large subunits , Rpb 1 , Rpb 2 , Rpb 3 and Rpb 5 . ^^^ |
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| Interacting proteins: P19388 and P24928 |
Pubmed |
SVM Score :0.0 |
| Previously we indicated that Rpb 3 also plays an essential role in subunit assembly because it interacts with at least four subunits , two large subunits ( Rpb 1 and Rpb 2 ) and two medium sized subunits ( Rpb 3 and Rpb 5 ) ( 1 ) , and it constitutes a core subassembly consisting of Rpb 2 , Rpb 3 , and Rpb 11 ( 2 ) . ^^^ |
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| Interacting proteins: P19388 and P24928 |
Pubmed |
SVM Score :0.0 |
| Mapping of Rpb 3 and Rpb 5 contact sites on two large subunits , Rpb 1 and Rpb 2 , of the RNA polymerase 2 from fission yeast . [ Rpb 1 and Rpb 2 ] Mapping of the contact sites on two large subunits of the fission yeast Schizosaccharomyces pombe RNA polymerase 2 with two small subunits , Rpb 3 and Rpb 5 , was carried out using the two hybrid screening system in the budding yeast Saccharomyces cerevisiae . ^^^ Rpb 5 was found to interact with any fragment of Rpb 1 that contained the region H , which is conserved among the subunit 1 homologues of all RNA polymerases , including the beta ' subunit of prokaryotic RNA polymerases . ^^^ |
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| Interacting proteins: P19388 and P24928 |
Pubmed |
SVM Score :0.0 |
| Notable features of the model include a pair of jaws , formed by subunits Rpb 1 , Rpb 5 , and Rpb 9 , that appear to grip DNA downstream of the active center . ^^^ |
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| Interacting proteins: P19388 and P24928 |
Pubmed |
SVM Score :0.0 |
| The RNA polymerase 2 ( Pol 2 ) of the fission yeast Schizosaccharomyces pombe is composed of 12 different polypeptides , Rpb 1 to Rpb 12 , of which five , Rpb 5 , Rpb 6 , Rpb 8 , Rpb 10 and Rpb 12 , are shared among three forms of the RNA polymerase . ^^^ |
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| Interacting proteins: P19388 and P24928 |
Pubmed |
SVM Score :0.0 |
| The transcription of one group of genes including rpb 3 , rpb 4 , rpb 5 , rpb 6 , rpb 7 and rpb 10 is mainly initiated at a single site , while that of the other group of genes for rpb 1 , rpb 2 , rpb 8 , rpb 9 , rpb 11 and rpb 12 is initiated at multiple sites . ^^^ |
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| Interacting proteins: P19388 and P24928 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : The RNA polymerase 2 of the fission yeast Schizosaccharomyces pombe consists of 12 Rpb subunits , of which four ( Rpb 1 , Rpb 2 , Rpb 3 and Rpb 11 ) form the assembly and catalytic core and five ( Rpb 5 , Rpb 6 , Rpb 8 , Rpb 10 and Rpb 12 ) are shared among RNA polymerases 1 , 2 and 3 . ^^^ |
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| Interacting proteins: P19388 and P24928 |
Pubmed |
SVM Score :0.0 |
| Nine subunits homologous to the other eukaryotic RNA Pol 2 , namely RPB 1 , RPB 2 , RPB 3 , RPB 4 , RPB 5 , RPB 6 , RPB 7 , RPB 8 and RPB 11 , were identified in the purified complex . ^^^ |
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