| Interacting proteins: P24928 and P19387 |
Pubmed |
SVM Score :0.0 |
| Mutations in the three largest subunits of yeast RNA polymerase 2 ( RPB 1 , RPB 2 , and RPB 3 ) were investigated for their effects on RNA polymerase 2 structure and assembly . ^^^ The time course of assembly of RPB 1 , RPB 2 , and RPB 3 was investigated in wild type cells and subsequently in mutant cells . ^^^ The RPB 2 plus RPB 3 subcomplexes accumulated in all RPB 1 assembly mutants at the nonpermissive temperature but not in an RPB 2 or RPB 3 assembly mutant . ^^^ These data indicate that RPB 2 and RPB 3 form a complex that subsequently interacts with RPB 1 during the assembly of RNA polymerase II . . ^^^ |
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| Interacting proteins: P24928 and P19387 |
Pubmed |
SVM Score :0.0 |
| Although multiple functions have been assigned to the three largest subunits , RPB 1 , RPB 2 , and RPB 3 , the functions of the remaining smaller subunits are unknown . ^^^ |
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| Interacting proteins: P24928 and P19387 |
Pubmed |
SVM Score :0.0 |
| Far Western blot analysis of S . pombe RNA polymerase 2 using 32P labelled recombinant Rpb 5 fused to glutathione S transferase ( GST ) as a probe , indicated that Rpb 5 binds strongly to membrane immobilized Rpb 1 , Rpb 2 and Rpb 3 and weakly to Rpb 5 and a 15 kDa subunit ( Rpb 8 or Rpb 11 ) . ^^^ In agreement with this result , the 32P labelled Rpb 3 probe showed a strong binding signal against Rpb 5 in addition to Rpb 1 and Rpb 2 . ^^^ |
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| Interacting proteins: P24928 and P19387 |
Pubmed |
SVM Score :0.0 |
| Previously , we cloned and sequenced both cDNA and the genes encoding the four large subunits , Rpb 1 , Rpb 2 , Rpb 3 and Rpb 5 . ^^^ |
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| Interacting proteins: P24928 and P19387 |
Pubmed |
SVM Score :0.0 |
| We introduced a histidine cluster tag sequence into the chromosomal rpb 1 and rpb 3 genes , which encode subunit 1 ( Rpb 1 ) and subunit 3 ( Rpb 3 ) , respectively , and purified the RNA polymerase by Ni2+ affinity chromatography . ^^^ After stepwise dissociation of the Rpb 1 and Rpb 3 tagged RNA polymerases fixed on Ni2+ resin by increasing concentrations of urea or guanidium hydrochloride , Rpb 2 Rpb3 Rpb 11 or Rpb 2 Rpb3 Rpb 11 Rpb10 complexes were obtained . ^^^ |
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| Interacting proteins: P24928 and P19387 |
Pubmed |
SVM Score :0.0 |
| Previously we indicated that Rpb 3 also plays an essential role in subunit assembly because it interacts with at least four subunits , two large subunits ( Rpb 1 and Rpb 2 ) and two medium sized subunits ( Rpb 3 and Rpb 5 ) ( 1 ) , and it constitutes a core subassembly consisting of Rpb 2 , Rpb 3 , and Rpb 11 ( 2 ) . ^^^ |
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| Interacting proteins: P24928 and P19387 |
Pubmed |
SVM Score :0.0 |
| Mapping of Rpb 3 and Rpb 5 contact sites on two large subunits , Rpb 1 and Rpb 2 , of the RNA polymerase 2 from fission yeast . [ Rpb 1 and Rpb 2 ] Mapping of the contact sites on two large subunits of the fission yeast Schizosaccharomyces pombe RNA polymerase 2 with two small subunits , Rpb 3 and Rpb 5 , was carried out using the two hybrid screening system in the budding yeast Saccharomyces cerevisiae . ^^^ |
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| Interacting proteins: P24928 and P19387 |
Pubmed |
SVM Score :0.0 |
| For the isolation of subunit complexes formed in the virus infected cells , a glutathione S transferase ( GST ) sequence was fused to the rpb 3 cDNA to produce GST Rpb 3 fusion protein and a decahistidine tag sequence was inserted into the rpb 1 cDNA to produce Rpb1H protein . ^^^ Coexpression of various combinations of a few subunits revealed that Rpb 11 enhances Rpb 3 Rpb8 interaction and consequently Rpb 8 enhances Rpb 1 Rpb3 interaction to some extent . ^^^ |
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| Interacting proteins: P24928 and P19387 |
Pubmed |
SVM Score :0.0 |
| Results indicate that the levels are low for the three large subunits , Rpb 1 , Rpb 2 and Rpb 3 , which are the homologues of beta ' , beta and alpha subunits , respectively , of prokaryotic RNA polymerase . ^^^ In agreement with the biochemical analysis , the GFP Rpb 1 and GFP Rpb 3 fusions were present in the nuclei but the GFP Rpb 4 was detected in the cytoplasm as well as the nuclei . . ^^^ |
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| Interacting proteins: P24928 and P19387 |
Pubmed |
SVM Score :0.0 |
| The transcription of one group of genes including rpb 3 , rpb 4 , rpb 5 , rpb 6 , rpb 7 and rpb 10 is mainly initiated at a single site , while that of the other group of genes for rpb 1 , rpb 2 , rpb 8 , rpb 9 , rpb 11 and rpb 12 is initiated at multiple sites . ^^^ Competitive PCR analysis indicated that mRNAs for Rpb 1 , Rpb 3 , Rpb 7 and Rpb 9 were among the group which had a low abundance , while the levels of Rpb 6 and Rpb 10 mRNAs were about fivefold , and that of Rpb 2 mRNA was about 40 fold higher than the Rpb 3 mRNA level . ^^^ The protein to mRNA ratio or the translation efficiency is low for the rpb 1 , rpb 2 , rpb 3 and rpb 11 genes , encoding the homologues of subunits beta ' , beta , alpha and alpha , respectively , of the prokaryotic RNA polymerase core enzyme . . ^^^ |
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| Interacting proteins: P24928 and P19387 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : The RNA polymerase 2 of the fission yeast Schizosaccharomyces pombe consists of 12 Rpb subunits , of which four ( Rpb 1 , Rpb 2 , Rpb 3 and Rpb 11 ) form the assembly and catalytic core and five ( Rpb 5 , Rpb 6 , Rpb 8 , Rpb 10 and Rpb 12 ) are shared among RNA polymerases 1 , 2 and 3 . ^^^ |
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| Interacting proteins: P24928 and P19387 |
Pubmed |
SVM Score :0.0 |
| Nine subunits homologous to the other eukaryotic RNA Pol 2 , namely RPB 1 , RPB 2 , RPB 3 , RPB 4 , RPB 5 , RPB 6 , RPB 7 , RPB 8 and RPB 11 , were identified in the purified complex . ^^^ |
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| Interacting proteins: P24928 and P19387 |
Pubmed |
SVM Score :0.0 |
| The cDNAs of five subunits ( hRPB 220 , hRPB 140 , hRPB 33 , hRPB 25 , and hRPB14 . 5 ) have been isolated . ^^^ |
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| Interacting proteins: P24928 and P19387 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P24928 and P19387 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24928 and P19387 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24928 and P19387 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24928 and P19387 |
Pubmed |
SVM Score :0.0 |
| NA |
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