| Interacting proteins: P19388 and P19387 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19388 and P19387 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19388 and P19387 |
Pubmed |
SVM Score :0.0 |
| The cDNAs of five subunits ( hRPB 220 , hRPB 140 , hRPB 33 , hRPB 25 , and hRPB14 . 5 ) have been isolated . ^^^ |
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| Interacting proteins: P19388 and P19387 |
Pubmed |
SVM Score :0.0 |
| Far Western blot analysis of S . pombe RNA polymerase 2 using 32P labelled recombinant Rpb 5 fused to glutathione S transferase ( GST ) as a probe , indicated that Rpb 5 binds strongly to membrane immobilized Rpb 1 , Rpb 2 and Rpb 3 and weakly to Rpb 5 and a 15 kDa subunit ( Rpb 8 or Rpb 11 ) . ^^^ In agreement with this result , the 32P labelled Rpb 3 probe showed a strong binding signal against Rpb 5 in addition to Rpb 1 and Rpb 2 . ^^^ CONCLUSION : Rpb 3 and Rpb 5 , the putative subunits of RNA polymerase 2 , associate each other to form binary complexes . ^^^ |
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| Interacting proteins: P19388 and P19387 |
Pubmed |
SVM Score :0.0 |
| Previously , we cloned and sequenced both cDNA and the genes encoding the four large subunits , Rpb 1 , Rpb 2 , Rpb 3 and Rpb 5 . ^^^ |
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| Interacting proteins: P19388 and P19387 |
Pubmed |
SVM Score :0.0 |
| Previously we indicated that Rpb 3 also plays an essential role in subunit assembly because it interacts with at least four subunits , two large subunits ( Rpb 1 and Rpb 2 ) and two medium sized subunits ( Rpb 3 and Rpb 5 ) ( 1 ) , and it constitutes a core subassembly consisting of Rpb 2 , Rpb 3 , and Rpb 11 ( 2 ) . ^^^ By making a set of Rpb 3 deletion derivatives , we carried out mapping of the Rpb 5 and Rpb 11 contact sites on Rpb 3 . ^^^ By far Western blot and GST pull down assays , we found that the amino acid sequence between residues 105 263 of Rpb 3 is involved in binding Rpb 5 , and the sequence between residues 105 297 is required for binding Rpb 11 . ^^^ Although the Rpb 5 and Rpb 11 contact sites on Rpb 3 overlap each other , both subunits are able to associate with Rpb 3 simultaneously . ^^^ The binding of Rpb 5 stabilizes the Rpb 3 Rpb11 heterodimer . . ^^^ |
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| Interacting proteins: P19388 and P19387 |
Pubmed |
SVM Score :0.0 |
| Mapping of Rpb 3 and Rpb 5 contact sites on two large subunits , Rpb 1 and Rpb 2 , of the RNA polymerase 2 from fission yeast . [ Rpb 1 and Rpb 2 ] Mapping of the contact sites on two large subunits of the fission yeast Schizosaccharomyces pombe RNA polymerase 2 with two small subunits , Rpb 3 and Rpb 5 , was carried out using the two hybrid screening system in the budding yeast Saccharomyces cerevisiae . ^^^ |
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| Interacting proteins: P19388 and P19387 |
Pubmed |
SVM Score :0.0 |
| After successive affinity chromatography on glutathione and Ni ( 2+ ) columns , complexes consisting of the seven subunits , Rpb1H , Rpb 2 , GST Rpb 3 , Rpb 5 , Rpb 7 , Rpb 8 and Rpb 11 , were identified . ^^^ |
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| Interacting proteins: P19388 and P19387 |
Pubmed |
SVM Score :0.0 |
| The transcription of one group of genes including rpb 3 , rpb 4 , rpb 5 , rpb 6 , rpb 7 and rpb 10 is mainly initiated at a single site , while that of the other group of genes for rpb 1 , rpb 2 , rpb 8 , rpb 9 , rpb 11 and rpb 12 is initiated at multiple sites . ^^^ |
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| Interacting proteins: P19388 and P19387 |
Pubmed |
SVM Score :0.0 |
| Only recently has it been suggested that besides the largest subunit of RNA polymerase 2 , smaller subunits like Rpb 3 and Rpb 5 may have regulatory roles in expression of specific sets of genes . ^^^ |
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| Interacting proteins: P19388 and P19387 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : The RNA polymerase 2 of the fission yeast Schizosaccharomyces pombe consists of 12 Rpb subunits , of which four ( Rpb 1 , Rpb 2 , Rpb 3 and Rpb 11 ) form the assembly and catalytic core and five ( Rpb 5 , Rpb 6 , Rpb 8 , Rpb 10 and Rpb 12 ) are shared among RNA polymerases 1 , 2 and 3 . ^^^ |
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| Interacting proteins: P19388 and P19387 |
Pubmed |
SVM Score :0.0 |
| Nine subunits homologous to the other eukaryotic RNA Pol 2 , namely RPB 1 , RPB 2 , RPB 3 , RPB 4 , RPB 5 , RPB 6 , RPB 7 , RPB 8 and RPB 11 , were identified in the purified complex . ^^^ |
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