| Interacting proteins: P19367 and P21796 |
Pubmed |
SVM Score :0.0 |
| Presented is a model for membrane associated hexokinase 1 , in which linear chains of hexokinase 1 dimers are stabilized by interactions with mitochondrial porin . . ^^^ |
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| Interacting proteins: P19367 and P21796 |
Pubmed |
SVM Score :0.0 |
| In brain tumors the main source of energy is from glycolysis , which is initiated by hexokinase 1 ( HK 1 ) , an enzyme bound to the mitochondrial porin . ^^^ |
|
| Interacting proteins: P19367 and P21796 |
Pubmed |
SVM Score :0.0 |
| This study examined the role of the N , N dicyclohexylcarbodiimide binding domain of mitochondrial porin in binding to hexokinase 1 . ^^^ Selective proteolytic cleavage of porin protein was performed and peptides were assayed for their , effect on hexokinase 1 binding to isolated mitochondria . ^^^ Specificity of DCCD reactive domain binding to hexokinase 1 was demonstrated by competition of the peptides for porin binding sites on hexokinase as well as by blockage hexokinase binding by N , N dicyclohexylcarbodiimide . ^^^ Preincubation of mitochondria with purified porin strongly increases hexokinase 1 binding to rat liver mitochondria . ^^^ |
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| Interacting proteins: P19367 and P21796 |
Pubmed |
SVM Score :0.0 |
| Specificity of HKCAT binding to mitochondria was demonstrated by competition of HK 1 for HKCAT binding sites on rat liver mitochondria as well as by blockage of HKCAT binding by N , N ' dicyclohexylcarbodiimide , which covalently binds to porin and blocks HK 1 binding . ^^^ |
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| Interacting proteins: P19367 and P21796 |
Pubmed |
SVM Score :0.0 |
| This apparent plasma membrane association appears unique , since somatic HK 1 is normally cytoplasmic or bound to the outer mitochondrial membrane via contact sites with a voltage dependent anion channel ( porin ) through a porin binding domain . ^^^ In male germ cells , three cDNA clones have been described that encode unique HK 1 isoforms ( HK 1 sa , HK 1 sb , HK 1 sc ) that do not contain porin binding domains ( Mori et al . , 1993 : Biol Reprod 49 : 191 203 ) . ^^^ |
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| Interacting proteins: P19367 and P21796 |
Pubmed |
SVM Score :0.0 |
| They encode a spermatogenic cell specific sequence region ( SSR ) but not the porin binding domain ( PBD ) necessary for HK 1 binding to porin on the outer mitochondrial membrane . ^^^ |
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| Interacting proteins: P19367 and P21796 |
Pubmed |
SVM Score :0.0 |
| Our previous study reported on our cloning of mouse Hk 1 s ( mHk 1 s ) cDNA , which were expressed only in testis cells , and noted that this cDNA has a spermatogenic cell specific region ( SSR ) that replaces the porin binding domain ( PBD ) in the Hk1of somatic cells . ^^^ |
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| Interacting proteins: P19367 and P21796 |
Pubmed |
SVM Score :0.0 |
| Glutamate 72 in VDAC 1 , shown previously to bind dicyclohexylcarbodiimide ( DCCD ) , which inhibits hexokinase isoform 1 ( HK 1 ) binding to mitochondria , was mutated to glutamine . ^^^ Binding of HK 1 to mitochondria expressing E72Q mVDAC 1 , as compared to native VDAC 1 , was decreased by approximately 70 % and rendered insensitive to DCCD . ^^^ HK 1 and ruthenium red ( RuR ) reduced the VDAC 1 conductance but not that of E72Q mVDAC 1 . ^^^ |
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| Interacting proteins: P19367 and P21796 |
Pubmed |
SVM Score :0.0 |
| These mRNAs contain a testis specific sequence not present in somatic cell HKI , but lack the sequence for the porin binding domain necessary for HKI to bind to porin on the outer mitochondrial membrane . ^^^ |
|
| Interacting proteins: P19367 and P21796 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19367 and P21796 |
Pubmed |
SVM Score :0.0 |
| NA |
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