| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.50011977 |
| Further downstream , in the absence of EphB 6 , ZAP 70 activation , LAT phosphorylation , the association of PLCgamma 1 with SLP 76 , and p44 / 42 MAPK activation were diminished . 0.50011977^^^ |
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| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| As SLP 76 interacts with both Grb 2 and phospholipase C gamma 1 , these data indicate that phosphorylation of SLP 76 by ZAP 70 provides an important functional link between the T cell receptor and activation of ras and calcium pathways . . ^^^ |
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| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| Uncoupling of nonreceptor tyrosine kinases from PLC gamma 1 in an SLP 76 deficient T cell . ^^^ SLP 76 was not required for TCR induced tyrosine phosphorylation of most proteins , but was required for optimal tyrosine phosphorylation and activation of phospholipase C gamma 1 ( PLC gamma 1 ) , as well as Ras pathway activation . ^^^ |
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| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| Using this cell line , we demonstrate that LAT is required for TCR mediated Ca2+ mobilization and optimal tyrosine phosphorylation of phospholipase C gamma 1 , Vav and SLP 76 . ^^^ |
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| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylation of phospholipase C gamma 1 ( but not of Syk ) and calcium mobilization in response to IgE cross linking were reduced in SLP 76 deficient BMMCs . ^^^ |
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| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| The study of molecules such as LAT , SLP 76 , FYB , SKAP 55 and VAV have revealed multiple mechanisms with which to control the activation of downstream signaling pathways through RAS , PLC gamma 1 and ERK / MAPK . ^^^ |
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| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| In this study , we identify a pathway in which Rlk phosphorylates SLP 76 leading to the phosphorylation of PLCgamma 1 , activation of ERKs , and the synergistic up regulation of TcR driven IL 2 NFAT / AP 1 transcription . ^^^ |
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| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| Importantly , CD 3 induced responses , such as tyrosine phosphorylation of cellular substrates ( LAT , SLP 76 , and PLC gamma 1 ) , as well as calcium mobilization , which are defective in T cells expressing neither ZAP 70 nor Syk , are observed in Syk ( hi ) / ZAP 70 ( ) T cells . ^^^ |
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| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| Whereas tyrosine phosphorylation of Fc ( epsilon ) RI , Syk , and Vav was intact in LAT deficient BMMCs following Fc ( epsilon ) RI engagement , tyrosine phosphorylation of SLP 76 , PLC gamma 1 , and PLC gamma 2 and calcium mobilization were dramatically reduced . ^^^ |
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| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| Following ligation of CD 2 on the surface of the Jurkat T cell line and human purified T cells , LAT was tyrosine phosphorylated and shown to associate in vivo with a number of other tyrosine phosphorylated proteins including PLCgamma 1 , Grb 2 , and SLP 76 . ^^^ Using Jurkat cell lines deficient in ZAP70 / Syk ( P 116 ) or LAT ( ANJ 3 ) expression , CD 2 dependent PLCgamma 1 and SLP 76 tyrosine phosphorylation required expression both of ZAP 70 or Syk and of LAT . ^^^ |
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| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylation of raft targeted PLCgamma 1 did not require Zap 70 or the interaction with the adapters Lat and Slp 76 , molecules that are necessary for TCR signaling . ^^^ |
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| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of LAT creates binding sites for the Src homology 2 ( SH 2 ) domains of other proteins , including PLC gamma 1 , Grb 2 , Gads , Grap , 3BP2 , and Shb , and indirectly binds SOS , c Cbl , Vav , SLP 76 , and Itk . ^^^ |
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| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| Because Vav 1 inducibly associates with further proteins including phospholipase C ( PLC ) gamma 1 and Src homology 2 domain containing leukocyte phosphoprotein of 76 kDa ( SLP 76 ) , we investigated their role for NF kappaB activation in Jurkat leukemia T cell lines deficient for expression of these two proteins . ^^^ Cells lacking SLP 76 or PLCgamma 1 failed to activate NF kappaB in response to T cell costimulation . ^^^ In contrast , replenishment of SLP 76 or PLCgamma 1 expression restored CD3 / CD28 induced IkappaB kinase ( IKK ) activity as well as NF kappaB DNA binding and transactivation . ^^^ PKCtheta activated NF kappaB in SLP 76 and PLCgamma 1 deficient cells , showing that PKCtheta is acting further downstream . ^^^ In contrast , Vav 1 induced NF kappaB activation was normal in SLP 76 ( ) cells , but absent in PLCgamma 1 ( ) cells . ^^^ |
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| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| Furthermore , mice possessing mutations in signaling molecules upstream of PLC gamma 1 , such as Src homology 2 ( SH 2 ) domain containing phosphoprotein of 76 kDa ( SLP 76 ) , linker for activation of T cells ( LAT ) and Vav 1 , or in members of the nuclear factor for activated T cells ( NFAT ) family of transcription factors , which are downstream of PLC gamma 1 , have been found to have similar phenotypes to Tec family deficient mice , emphasizing the importance of this pathway in regulating T cell activation , differentiation and homeostasis . . ^^^ |
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| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| T cell receptor induced activation of phospholipase C gamma 1 depends on a sequence independent function of the P 1 region of SLP 76 . ^^^ T cell receptor ( TCR ) induced activation of phospholipase C gamma 1 ( PLC gamma 1 ) depends on three features of SLP 76 : the N terminal tyrosine phosphorylation sites , the Gads binding site , and an intervening sequence , denoted the P 1 region , which binds to the SH 3 domain of PLC gamma 1 ( SH 3 ( PLC ) ) via a low affinity interaction . ^^^ Despite extensive research , the mechanism whereby SLP 76 regulates PLC gamma 1 remains uncertain . ^^^ To better understand the contribution of the P 1 region to PLC gamma 1 activation , we mapped the PLC gamma 1 binding site within the region , and created a SLP 76 mutant that fails to bind SH 3 ( PLC ) , but is fully functional , mediating TCR induced phosphorylation of PLC gamma 1 at tyrosine 783 , calcium flux , and nuclear factor of activated T cells activation . ^^^ Our results demonstrate that SLP 76 need not interact with SH 3 ( PLC ) to activate PLC gamma 1 , and further suggest that the P 1 region of SLP 76 serves a structural role that is sequence independent and is not directly related to protein protein interactions . . ^^^ |
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| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| In addition , incubation of activated CD8+ T cells with p 815 cells expressing 4 1BBL led to redistribution of the lipid raft domains and Lck , protein kinase C theta ; , SLP 76 , and phospholipase C gamma 1 ( PLC gamma 1 ) on the T cell membranes to the areas of contact with the p 815 cells and recruitment of 4 1BB , TNFR associated factor 2 , and phospho tyrosine proteins to the raft domains . 4 1BB ligation also caused translocation of TNFR associated factor 2 , protein kinase C theta ; , PLC gamma 1 , and SLP 76 to detergent insoluble compartments in the CD8+ T cells , and cross linking of 4 1BB increased intracellular Ca2+ levels apparently by activating PLC gamma 1 . ^^^ |
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| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| Z / 3 Cbl induces PLC gamma 1 activation in T lymphocytes via an alternate Lat and Slp 76 independent signaling mechanism . ^^^ Furthermore , 70Z / 3 Cbl induced activation of NFAT , a PLC gamma 1 / Ca ( 2+ ) dependent transcriptional event , required Zap 70 , but was independent of Slp 76 , an adapter required for TCR induced NFAT activation . ^^^ These results suggest that 70Z / 3 Cbl and PLC gamma 1 form a TCR , Lat and Slp 76 independent complex that leads to PLC gamma 1 phosphorylation and activation . . ^^^ |
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| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| Thr 155 phosphorylation reduces the ability of LAT to recruit PLCgamma 1 and SLP 76 , leading to attenuation of subsequent downstream events such as [ Ca2+ ] 1 mobilization and activation of the ERK pathway . ^^^ |
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| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| Of particular interest , the PLC gamma 1 SH3 domain was found to associate with SLP 76 through at least three distinct sites , two of which bore a novel KKPP motif and the other contained the classic PXXP sequence . ^^^ Intriguingly mutation of critical residues for the three sites not only affected binding of SLP 76 to the PLC gamma 1 SH3 domain but also to the Grb 2 C terminal SH 3 domain , indicating that the binding sites in SLP 76 for the two SH 3 domains are overlapped . ^^^ |
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| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| Following T cell receptor ligation , PLCgamma 1 interacts through its SH 2 and SH 3 domains with the adaptors LAT and SLP 76 , respectively , to form a multiprotein signaling complex that leads to activation of PLCgamma 1 by Syk tyrosine kinases . ^^^ To identify the binding site for PLCgamma 1 in SLP 76 , we used isothermal titration calorimetry to measure affinities for the interaction of PLCgamma 1 SH3 with a set of overlapping peptides spanning the central proline rich region of SLP 76 . ^^^ PLCgamma 1 SH3 bound with high specificity to the SLP 76 motif 186PPVPPQRP193 , which represents the minimal binding site . ^^^ In addition , Pro 193 of SLP 76 assists in positioning Arg 192 into the compass pocket of PLCgamma 1 SH3 , which coordinates the compass residue through an unusual aspartate . ^^^ The PLCgamma 1 SH3 / SLP 76 structure provides insights into ligand binding by SH 3 domains related to PLCgamma 1 SH3 , as well as into recognition by PLCgamma 1 of signaling partners other than SLP 76 . . ^^^ |
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| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| Either the SH 3 or C terminal SH 2 domain of PLCgamma 1 , with the participation of Vav 1 , c Cbl and Slp 76 , are required to stabilize PLCgamma 1 recruitment . ^^^ |
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| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13094 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
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