Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.84528235
The association of PLC gamma 1 with GRB 2 and Sos suggests that PLC gamma 1 may be directly involved in the Ras signaling pathway and that GRB 2 may be involved in the translocation of PLC gamma 1 from cytosol to the plasma membrane as a necessary step for its effect on inositol lipid hydrolysis . . 0.84528235^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.68128451
Furthermore , we demonstrate interaction of PLCgamma 1 with Grb 2 and SOS 2 . 0.68128451^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.91847135
In this study , we demonstrate for the first time that Grb 2 , an adaptor protein , specifically interacts with tyrosine phosphorylated PLC gamma 1 at Tyr 783 . 0.91847135^^^ The association of Grb 2 with PLC gamma 1 was induced by the treatment with epidermal growth factor ( EGF ) . 0.91094547^^^ Grb 2 negatively regulates epidermal growth factor induced phospholipase C gamma 1 activity through the direct interaction with tyrosine phosphorylated phospholipase C gamma 1 . 0.87475722^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
Treatment of cells with diacylglycerol ( DAG ) or DAG and microinjected inositol 1 , 4 , 5 triphosphate ( IP 3 ) , the products of activated PLC gamma 1 , did not stimulate cellular DNA synthesis by themselves but did suppress the inhibitory effects of the PLC gamma 1 SH2 SH 2 SH3 polypeptide but not the cell cycle block imposed by inhibition of the adapter protein Grb 2 or p 21 Ras . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
GRB 2 and phospholipase C gamma 1 associate with a 36 to 38 kilodalton phosphotyrosine protein after T cell receptor stimulation . ^^^ Furthermore , following partial immunodepletion of GRB 2 and of the associated pp 36 38 , there was a significant reduction in the amount of the 36 kDa phosphoprotein associated with PLC gamma 1 , suggesting that a trimeric PLC gamma 1 / pp36 38 / GRB2 complex could form . ^^^ In support of this notion , we have also been able to detect low levels of PLC gamma 1 in GRB 2 immunoprecipitates . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
The tyrosine phosphorylated form of pp36 / 38 is membrane associated and directly interacts with phospholipase C gamma 1 and Grb 2 , providing one mechanism to recruit downstream effectors to the cell membrane . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
Ligation of the T cell antigen receptor ( TCR ) induces association of hSos 1 , ZAP 70 , phospholipase C gamma 1 , and other phosphoproteins with Grb 2 and the zeta chain of the TCR . ^^^ In addition to hSos 1 , Grb 2 participates in the formation of a tyrosine phosphoprotein complex that includes 145 , 95 , 70 , 54 , and 36 38 kDa proteins . p 145 was identified as PLC gamma 1 and p 70 as the protein tyrosine kinase , ZAP 70 . ^^^ The SH 2 domains of Grb 2 and PLC gamma 1 were required for the formation of this protein complex . ^^^ In anti CD 3 treated cells , Grb 2 redistributed from the cytosol to a particulate cell compartment along with p36 / p38 , ZAP 70 , and PLC gamma 1 . ^^^ Taken together , these results indicate that anti CD 3 induces Grb 2 hSos1 PLC gamma 1 p36 / p38 ZAP 70 complexes , which localize in the vicinity of TCR zeta . . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
We show that phospholipase C gamma 1 , Ras GTPase activating protein , the p 85 subunit of phosphatidylinositol 3 ' kinase , Shc , Grb 2 , Vav , Fyn , and Src are components of the Flk2 / Flt3 signal transduction pathway . ^^^ In addition , we demonstrate that phospholipase C gamma 1 , the p 85 subunit of phosphatidylinositol 3 ' kinase , Shc , Grb 2 , and Src family tyrosine kinases , but not Ras GTPase activating protein , Vav , or Nck , physically associate with the Flk2 / Flt3 cytoplasmic domain . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
Association of p 62 , a multifunctional SH 2 and SH 3 domain binding protein , with src family tyrosine kinases , Grb 2 , and phospholipase C gamma 1 . src family tyrosine kinases contain two noncatalytic domains termed src homology 3 ( SH 3 ) and SH 2 domains . ^^^ Here we show that the SH 3 and / or SH 2 domains of the signalling proteins Grb 2 and phospholipase C gamma 1 can interact with p 62 both in vitro and in vivo . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
In this study we examine the ability of tyrosine phosphorylated proteins from Jurkat T cells stimulated by CD 2 or T cell receptor CD 3 to interact with the src homology 2 or src homology 3 domains from eight different proteins involved in signal transduction in lymphocytes : Vav , Shc , Nck , phosphatidylinositol 3 kinase , phospholipase C gamma 1 , Ras GTPase activating protein , c Crk and Grb 2 . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
Cloning and characterization of Lnk , a signal transduction protein that links T cell receptor activation signal to phospholipase C gamma 1 , Grb 2 , and phosphatidylinositol 3 kinase . ^^^ In activated T lymphocytes , phospholipase C gamma 1 , phosphatidylinositol 3 kinase , and Grb 2 coimmunoprecipitated with Lnk . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
Tyrosine phosphorylation of Grb 2 associated proteins correlates with phospholipase C gamma 1 activation in T cells . ^^^ These data demonstrate that tyrosine phosphorylation of a protein ( s ) which binds the SH 2 domain of Grb 2 correlates with phospholipase C gamma 1 activation and suggest that such a phosphoprotein ( s ) plays a critical role in coupling the TCR with the phosphatidylinositol second messenger pathway . . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
Signal transduction of fibroblast growth factor ( FGF ) receptors is known to involve tyrosine phosphorylation of several substrates , including Grb 2 , phospholipase C gamma , and phosphatidylinositol 3 kinase , whereas the role of G proteins in FGF receptor signaling is controversial . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
As SLP 76 interacts with both Grb 2 and phospholipase C gamma 1 , these data indicate that phosphorylation of SLP 76 by ZAP 70 provides an important functional link between the T cell receptor and activation of ras and calcium pathways . . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
Tyrosine phosphorylated beta and gamma ITAM peptides immobilized on beads precipitated Syk , Lyn , Shc , Grb 2 , and phospholipase C gamma 1 from lysates of rat basophilic leukemia RBL 2H3 cells . ^^^ Phospholipase C gamma 1 , Shc , and Grb 2 were precipitated only by the diphosphorylated beta ITAM peptide . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
Purified amino terminal Src homology 2 ( SH 2 ) domains of GAP , PLCgamma 1 and the p85alpha subunit of PI 3 kinase , as well as the carboxy terminal SH 2 domain of the latter protein and the unique SH 2 domain of Grb 2 , were injected into full grown , stage 6 Xenopus laevis oocytes . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
Following binding of platelet derived growth factor ( PDGF ) , the PDGF alpha receptor ( alphaPDGFR ) becomes tyrosine phosphorylated and associates with a number of signal transduction molecules , including phospholipase Cgamma 1 ( PLCgamma 1 ) , phosphatidylinositol 3 kinase ( PI3K ) , the phosphotyrosine phosphatase SHP 2 , Grb 2 , and Src . ^^^ Characterization of the F 720 mutant indicated that binding of two proteins , SHP 2 and Grb 2 , was severely impaired , whereas PLCgamma 1 and PI3K associated to wild type levels . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
The 68 kDa protein was coimmunoprecipitated with various signaling proteins such as phospholipase C gamma 1 , the phosphatidylinositol 3 kinase p 85 subunit , Grb 2 , SHP 1 , Cbl , and Jak 3 , implying that the protein might function as an adapter . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
Previous studies suggested that Lnk , a 38 kDa protein consisting of a single SH 2 domain and a region containing potential tyrosine phosphorylation sites , might serve to join Grb 2 , phospholipase C gamma 1 , and phosphatidylinositol 3 kinase to the TCR . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
Tyrosine phosphorylation of EGF receptor ( EGFR ) , Shc , and phospholipase C gamma 1 ( PLC gamma ) , and growth factor receptor binding protein 2 ( Grb 2 ) coprecipitation with EGFR and Shc were analyzed by Western blotting . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
We describe here two tyrosine phosphorylated proteins with Mr values of 70 , 000 and 68 , 000 that interact with Grb 2 , phospholipase C ( PLCgamma 1 and PLCgamma 2 ) , and Vav after B cell receptor cross linking . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
Here we report the cloning of the cDNA for a highly tyrosine phosphorylated 36 38 kDa protein , previously characterized by its association with Grb 2 , phospholipase C gamma 1 , and the p 85 subunit of phosphoinositide 3 kinase . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
In a previous report , we demonstrated that PLC gamma 1 , Grb 2 , SOS and Nck were associated with activated FGFR1s in a signaling complex in Xenopus blastulae . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
In addition , the PLC gamma 1 SH 2 ( N ) domain mutant failed to associate with Grb 2 and a 36 to 38 kDa phosphoprotein ( p 36 38 ) , which has previously been recognized to interact with PLC gamma 1 , Grb 2 , and other molecules involved in TCR signal transduction . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
During mitosis , Sam 68 undergoes tyrosine phosphorylation , which negatively regulates its nucleic acid binding properties and mediates the interaction of Sam 68 with critical SH 2 containing signaling proteins such as Grb 2 , PLC gamma 1 and Ras GAP . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
Thus , we examined the interactions of various SH 2 containing molecules like PLC gamma 1 , rasGAP , p 85 subunit of PI 3 kinase , Src , Fyn , Crk , Nck , Grb 2 and Shc with HEK 2 using in vitro binding assays , immunoprecipitations and yeast Two Hybrid assays . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
Cbl is an adapter like protein known to interact with several SH 3 domains , including the PLCgamma 1 SH3 domain and the Grb 2 amino terminal SH 3 domain . ^^^ Consistent with previous reports , our results demonstrated a weak binding of Cbl to GST fusion proteins that strictly encompass the structural core of the PLCgamma 1 SH3 domain but a high avidity binding to the Grb 2 amino terminal SH 3 domain . ^^^ Inclusion of amino acids immediately flanking the PLCgamma 1 SH3 core domain , however , substantially increased binding of Cbl to a level comparable to that of the Grb 2 amino terminal SH 3 domain . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
A GST fusion protein containing the SH 3 domain of the Grb 2 adaptor protein does not inhibit tr kit induced egg activation , showing that the effect of the SH 3 domain of PLCgamma 1 is specific . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
Phosphorylated LAT binds Grb 2 , PLC gamma 1 , and other signaling molecules . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
In addition to Zap 70 , the 3BP2 SH 2 domain associated in vitro with LAT , Grb 2 , PLCgamma 1 , and Cbl from activated T cell lysates . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
SLM 1 bound the SH 2 and SH 3 domains of p 59 ( fyn ) , Grb 2 , phospholipase Cgamma 1 ( PLCgamma 1 ) , and / or p 120 ( rasGAP ) , suggesting it may function as a multifunctional adapter protein for Src during mitosis . ^^^ Moreover , SLM 2 did not associate with the SH 3 domains of p 59 ( fyn ) , Grb 2 , PLCgamma 1 , or p 120 ( rasGAP ) , suggesting that SLM 2 may not function as an adapter protein for these proteins . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
Finally , the structure of Grb 2 SH2 domain was compared with those of the complexes of Src and phospholipase C gamma 1 with their cognate peptides , showing that the specific conformation of the peptide was required for binding to the SH 2 domains . . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
We found that CD 44 cross linking by mAb in CD 4 ( + ) peripheral blood T cells promotes formation of a trimeric complex of Grb 2 , phospholipase ( PLC ) gamma 1 and a 36 38 kDa phosphoprotein , and the activation of PLC gamma 1 . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
LAT ( linker for activation of T cells ) is an adaptor molecule , which following its phosphorylation associates with Grb 2 , Gads , PLC gamma 1 , and other signalling molecules . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
Co immunoprecipitation experiments revealed a constitutive association of p 36 LAT with both FcgammaRI and FcgammaRIIa immunoprecipitates , and an activation induced association of LAT with PLCgamma 1 , Grb 2 , and the p 85 subunit of phosphatidylinositol 3 kinase . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
Association of Grb 2 , Gads , and phospholipase C gamma 1 with phosphorylated LAT tyrosine residues . ^^^ Upon T cell activation , LAT becomes highly phosphorylated on tyrosine residues , and Grb 2 , Gads , and phospholipase C ( PLC ) gamma 1 bind LAT via Src homology 2 domains . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
The Grb 2 adapter protein was detected in complex with hSos / PLCgamma 1 , suggesting a regulatory role for Grb 2 . ^^^ SH 3 domains from both Grb 2 and PLCgamma 1 , but not RasGAP , bound directly to hSos homologues . ^^^ The SH 2 domain from Grb 2 formed an association with the hSos / PLCgamma 1 complex , which was enhanced following TCR / CD 3 ligation . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
Following ligation of CD 2 on the surface of the Jurkat T cell line and human purified T cells , LAT was tyrosine phosphorylated and shown to associate in vivo with a number of other tyrosine phosphorylated proteins including PLCgamma 1 , Grb 2 , and SLP 76 . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
Following TCR stimulation , LAT becomes tyrosine phosphorylated , creating docking sites for other signaling proteins such as phospholipase C gamma ( 1 ) ( PLC gamma ( 1 ) ) , Grb 2 , and Gads . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
In the Jurkat T cell line , the distal four tyrosines of LAT bind PLCgamma 1 , Grb 2 , and Gads . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
In addition , using this system , we found the interaction between the adaptor protein , Lad , and the SH 2 domain of Grb 2 or PLC gamma 1 . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
Phosphorylation of LAT creates binding sites for the Src homology 2 ( SH 2 ) domains of other proteins , including PLC gamma 1 , Grb 2 , Gads , Grap , 3BP2 , and Shb , and indirectly binds SOS , c Cbl , Vav , SLP 76 , and Itk . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
Tyrosine phosphorylation stimulates PI3K and PLCgamma 1 enzymatic activity , and on ShcA creates binding sites for Grb 2 with its associated Sos 1 and Gab 1 . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
Second , disruption of the interaction of the insulin receptor with PLC gamma 1 by microinjection of SH 2 domains derived from PLC gamma 1 or Grb 2 but not Shc similarly blocks insulin induced DNA synthesis . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
In addition , the reconstituted WASP interacted in vitro with proteins containing SH 3 domain such as Grb 2 , PLC gamma 1 , and Fyn , each of which are connected to signaling pathways linked to the actin cytoskeleton . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
Upon T cell antigen receptor ( TCR / CD3 ) engagement , LAT is phosphorylated by protein tyrosine kinases ( PTK ) and binds to the adaptors Gads and Grb 2 , as well as to phospholipase Cgamma 1 ( PLCgamma 1 ) , thereby facilitating the recruitment of key signal transduction components to drive T cell activation . ^^^ The LAT tyrosine residues Y ( 132 ) , Y ( 171 ) , Y ( 191 ) , and Y ( 226 ) have been shown previously to be critical for binding to Gads , Grb 2 , and PLCgamma 1 . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
Grb 2 , Gads , and PLC gamma 1 associated with LAT preferentially via different sets of tyrosine residues ; however , they failed to interact with LAT mutants containing only one tyrosine . ^^^ LAT mutants that were capable of binding Grb 2 and PLC gamma 1 could reconstitute T cell activation in LAT deficient cells and thymocyte development in LAT deficient mice . . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
HGF stimulated Gab 1 association with c Met , Grb 2 , SHP 2 , PI3K , Shc , Crk isoforms and CrkL , but not with PLCgamma 1 . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
However , even at concentrations of 0 . 1 to 1000 microM , no significant inhibitions were observed against other SH 2 domains binding such as the growth factor receptor binding protein 2 ( Grb 2 ) SH 2 domain to phosphopeptide of Shc and phospholipase Cgamma 1 ( PLCgamma 1 ) SH 2 domain to translational elongation factor 1alpha ( EF1alpha ) C terminal . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
After phosphorylation of the cytosolic domain of LAT , multiple signaling molecules such as phospholipase C gamma 1 , Grb 2 , and Gads associate with phosphorylated LAT via their SH 2 domains . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
By using this system , we showed that endosomal activation of PDGFR recruits various signaling proteins including Grb 2 , SHC , phospholipase C gamma 1 , and the p85alpha subunit of phosphatidylinositol 3 kinase into endosomes and forms signaling complexes with PDGFR . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
Previously reported proteins in the ALK signal pathway were identified including PI 3 K , Jak 2 , Jak 3 , Stat 3 , Grb 2 , IRS , and PLCgamma 1 . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
Additionally we identified proteins that are novel candidates for mediating the cellular responses to CD 437 ( e . g . , FAF 1 , Bid , caspase 8 , cdk 1 , KAP , NDR , RBBP , 53BP2 , Grb 2 , PLCgamma 1 , p70s6k , PP2Cdelta , PKBalpha / AKT , PDK 1 , and several DNA repair enzymes ) . . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
Expression of a partially activated A Raf mutant resulted in decreased tyrosine phosphorylation of the PDGFR , specifically on Y 857 ( autophosphorylation site ) and Y 1021 ( phospholipase Cgamma 1 ( PLCgamma 1 ) binding site ) , but not the binding sites for other signalling proteins ( Nck , phosphatidylinositol 3 ' kinase ( PI3K ) , RasGAP , Grb 2 , SHP ) . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
Intriguingly mutation of critical residues for the three sites not only affected binding of SLP 76 to the PLC gamma 1 SH3 domain but also to the Grb 2 C terminal SH 3 domain , indicating that the binding sites in SLP 76 for the two SH 3 domains are overlapped . ^^^
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P19174	Pubmed	SVM Score :0.0
NA