| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| The SH 2 domains of ZAP 70 , SHP 1 and PLC gamma 1 and the SH 3 domains of lck , vav and HS 1 did not bind to pp 85 90 . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| Ligation of the T cell antigen receptor ( TCR ) induces association of hSos 1 , ZAP 70 , phospholipase C gamma 1 , and other phosphoproteins with Grb 2 and the zeta chain of the TCR . ^^^ In addition to hSos 1 , Grb 2 participates in the formation of a tyrosine phosphoprotein complex that includes 145 , 95 , 70 , 54 , and 36 38 kDa proteins . p 145 was identified as PLC gamma 1 and p 70 as the protein tyrosine kinase , ZAP 70 . ^^^ In anti CD 3 treated cells , Grb 2 redistributed from the cytosol to a particulate cell compartment along with p36 / p38 , ZAP 70 , and PLC gamma 1 . ^^^ Taken together , these results indicate that anti CD 3 induces Grb 2 hSos1 PLC gamma 1 p36 / p38 ZAP 70 complexes , which localize in the vicinity of TCR zeta . . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| Crosslinking Fc gamma RIIA with anti Fc gamma RII monoclonal antibody also induced tyrosine phosphorylation of multiple proteins including Fc gamma RIIA , ZAP 70 , p72SYK , and phospholipase C gamma 1 subunit and an increase in intracellular Ca2+ concentration . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| The proteins that became highly tyrosine phosphorylated included phospholipase C ( PLC gamma 1 ) , p95vav , ZAP 70 , the MAP kinase , CD 3 zeta and unidentified proteins of 120 , 100 and 80 kDa . ^^^ These data suggest that PLC gamma 1 , PLC gamma 1 associated molecules , p95vav , the 80 kDa protein , ZAP 70 and the CD 3 zeta chain may be substrates of p59fyn or of other tyrosine kinases regulated by p59fyn and be important in T cell signaling . . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| Expression of the zeta chain is required for functional synergy of the T cell receptor with CD 4 in the activation of phospholipase C gamma 1 , which probably reflects an interaction between p56lck and zeta associated kinase ZAP 70 . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| TCR mediated Ras activation , tyrosine phosphorylation and translocation of cellular proteins including ZAP 70 , PLC gamma 1 , and PKC . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| As SLP 76 interacts with both Grb 2 and phospholipase C gamma 1 , these data indicate that phosphorylation of SLP 76 by ZAP 70 provides an important functional link between the T cell receptor and activation of ras and calcium pathways . . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| Interestingly , the SH 2 domains of PLCgamma 1 associate with ZAP 70 upon CD 3 stimulation while they bind , in CD 2 activated cells , to a heavily tyrosine phosphorylated 62 kDa protein . ^^^ Altogether , these findings suggest that CD 2 could bypass the PTK ZAP 70 for PLCgamma 1 activation and involves a preferential cascade comprising p56lck and a p 62 protein , possibly acting as an anchor molecule . . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| Results demonstrate that in BW 5147 wild type TCR , tail less zeta TCR , CD3epsilon , and TCRzeta transduce signals leading to tyrosine phosphorylation of similar sets of cellular substrates , including the receptor subunits , Fyn , ZAP 70 , and phospholipase Cgamma 1 ( PLCgamma 1 ) . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| These data indicated that in Jurkat T cells , CD 38 receptor triggering results in Lck regulated activation of both Raf 1 / MAP kinase and CD 3 zeta / ZAP 70 / phospholipase C gamma 1 signaling pathways . . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| We demonstrate that NOD T cell hyporesponsiveness is associated with the ( a ) enhanced TCR beta associated Fyn kinase activity and the differential activation of the Fyn TCR zeta Cbl pathway , which may account for the impaired recruitment of ZAP 70 to membrane bound TCR zeta ; ( b ) relative inability of the murine son of sevenless ( mSOS ) Ras GDP releasing factor activity to translocate from the cytoplasm to the plasma membrane ; and ( c ) exclusion of mSOS and PLC gamma 1 from the TCR zeta associated Grb2 / pp36 38 / ZAP70 signaling complex . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| To test this , binding studies were conducted with tandem domains from the five signaling enzymes : phosphatidylinositol 3 kinase p 85 , ZAP 70 , Syk , SHP 2 , and phospholipase C gamma 1 . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| There are significant reductions in anti epsilon induced tyrosine phosphorylation of zeta , epsilon , ZAP 70 , and phospholipase C gamma 1 , as well as in intracellular Ca2+ mobilization . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| Expression of p 56 ( lck ) and Zap 70 , but not phospholipase C gamma 1 , was reduced in these patients ' T cells relative to those obtained from normal individuals . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| In addition to Zap 70 , the 3BP2 SH 2 domain associated in vitro with LAT , Grb 2 , PLCgamma 1 , and Cbl from activated T cell lysates . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of Tyr 319 in ZAP 70 is required for T cell antigen receptor dependent phospholipase C gamma 1 and Ras activation . ^^^ Phosphorylation of Tyr 319 promoted the association of ZAP 70 with the SH 2 domains of two key signaling molecules , Lck and PLC gamma 1 . ^^^ These studies suggest that Tyr 319 phosphorylation is required for the assembly of a ZAP 70 containing signaling complex that leads to the activation of the PLC gamma 1 and Ras dependent signaling cascades in antigen stimulated T cells . . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| Coordinate activation of these pathways is dependent on Lck and ZAP 70 mediated tyrosine phosphorylation of a 36 kDa linker for activation of T cells and subsequent recruitment of phospholipase C gamma 1 , Grb 2 SOS , and SLP 76 vav . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| Importantly , CD 3 induced responses , such as tyrosine phosphorylation of cellular substrates ( LAT , SLP 76 , and PLC gamma 1 ) , as well as calcium mobilization , which are defective in T cells expressing neither ZAP 70 nor Syk , are observed in Syk ( hi ) / ZAP 70 ( ) T cells . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| Using Jurkat cell lines deficient in ZAP70 / Syk ( P 116 ) or LAT ( ANJ 3 ) expression , CD 2 dependent PLCgamma 1 and SLP 76 tyrosine phosphorylation required expression both of ZAP 70 or Syk and of LAT . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| This review highlights the use of mutant T cell lines for functional characterizations of two of these signaling proteins the ZAP 70 tyrosine kinase and phospholipase C gamma1 . . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylation of raft targeted PLCgamma 1 did not require Zap 70 or the interaction with the adapters Lat and Slp 76 , molecules that are necessary for TCR signaling . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| In this study , we investigated the participation of Lck and ZAP 70 protein tyrosine kinases ( PTKs ) , as well as PLC gamma 1 and Shc proteins in PBMC activation by Schistosoma mansoni antigens . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| Moreover , the signal supplied by CD 28 does not lead to increased Zap 70 and Lat phosphorylation , but amplifies PLCgamma 1 activation and Ca ( 2+ ) response . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylation of PLC gamma 1 is largely dependent on 3 kinds of protein tyrosine kinases ( PTKs ) , ie , Lck , ZAP 70 , and Itk . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| We analyzed jacalin induced intracellular signaling events in CD 4 ( + ) T cells and have shown that cell activation resulted in tyrosine phosphorylation of intracellular substrates p 56 ( lck ) , p 59 ( fyn ) , ZAP 70 , p 95 ( vav ) , phospholipase C gamma 1 , and ras activation , as assessed by conversion of ras guanosine 5 ' diphosphate to ras guanosine 5 ' triphosphate . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| The complete elimination of PLC gamma 1 phosphorylation required deleting the SH 3 domain binding region of 70Z / 3 Cbl , consistent with 70Z / 3 Cbl binding the PLC gamma 1 SH 3 domain . 70Z / 3 Cbl induced PLC gamma 1 phosphorylation required Zap 70 , as for the TCR , and the tyrosine kinase binding domain of 70Z / 3 Cbl , which binds Zap 70 , but did not require PLC gamma 1 binding to Lat , a crucial interaction in TCR induced PLC gamma 1 phosphorylation . ^^^ Furthermore , 70Z / 3 Cbl induced activation of NFAT , a PLC gamma 1 / Ca ( 2+ ) dependent transcriptional event , required Zap 70 , but was independent of Slp 76 , an adapter required for TCR induced NFAT activation . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| Taken together , these results suggest that rosmarinic acid has the potential to specifically inhibit Lck SH 2 domain binding to its cognate ligand , including ZAP 70 , Cbl , HS 1 , and PLCgamma 1 , and Lck dependent Ca 2+ signaling pathway of its downstream effector and finally to modulate IL 2 gene expression after T cell activation . . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| We identified known signaling molecules such as Lck , ZAP 70 , Syk , PLC gamma 1 and SHP 1 ( PTP1C ) as truncation mutants with dominant negative or constitutively active functions . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| The T cell receptor zeta chain is required for efficient receptor expression and contributes to T cell receptor mediated activation of ZAP 70 and PLC gamma 1 as well as other signaling functions . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| Crosslinking of purified wild type nave CD 4 cells with anti CD 3 activated Lck and initiated the signaling cascade downstream of Lck , including phosphorylation of ZAP 70 , LAT , and PLC gamma 1 ; calcium flux ; and dephosphorylation and nuclear translocation of the nuclear factor of activated T cells ( NFAT ) p . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| Further downstream , in the absence of EphB 6 , ZAP 70 activation , LAT phosphorylation , the association of PLCgamma 1 with SLP 76 , and p44 / 42 MAPK activation were diminished . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| Absence of WASp results in decreased accumulation of calcineurin , WASp interacting protein , and molecules upstream of calcium mobilization , i . e . , activated ZAP 70 and phospholipase C gamma 1 , in the disorganized NK cell immune synapse . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| In additions , we provide evidence that ZAP 70 is the primary in vivo kinase for LAT tyrosine 191 and that Itk plays a role in the phosphorylation of tyrosine 783 on phospholipase C gamma 1 . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| In OPAL stimulated T cells , PLC gamma 1 was phosphorylated and it was suppressed by DN ZAP 70 expression , suggesting that the ZAP 70 TCR zeta association mediates the activation of PLC gamma 1 leading to T cell responses even in the absence of kinase activation of ZAP 70 . . ^^^ |
|
| Interacting proteins: P43403 and P19174 |
Pubmed |
SVM Score :0.0 |
| Signaling events downstream of LAT ( PLCgamma 1 phosphorylation and p 85 [ PI 3 K ] association ) were impaired , whereas upstream events ( CD3zeta and ZAP 70 phosphorylation ) remained intact . ^^^ |
|